KR20190012324A - Seaweed extract for treating skin inflammatory diseases and composition containing the same - Google Patents

Abstract

The present invention relates to a kelp extract for treating skin inflammatory diseases containing alginic acid and fucoidan extracted from kelp and a composition containing the same, which contains at least one selected from sodium hyaluronate, butylene glycol, glycerin, squalene and jojoba oil.

Description

SEAWEED EXTRACT FOR SKIN INFLAMMATORY DISEASE AND COMPOSITION COMPRISING THE SAME

The present invention relates to seaweed extracts for treating skin inflammatory diseases and compositions containing the same, and more particularly, to alginic acid, fucoidan and compositions containing the same, which are extracted from sea tangle and contain sodium hyaluronate, butyleneglycol, glycerin, squalane, Jojoba oil, and the like, and relates to a composition containing the sea tangle extract for treating skin inflammatory disease.

Skin is the most extraterrestal organs of the human body. It protects the body's moisture and blocks invading factors from the outside, thus acting as an essential barrier to the human body. Factors that cause skin irritation include physicochemical stimuli, allergens, ultraviolet rays, oxidative stress, pathogenic infections and secondary inflammation-related cytokines and chemokines.

The common pathway of skin inflammation is the immune response to protect the body from external stimuli, and the control of inflammation through inhibition of inflammation has become a major target of therapy. In the lesion of skin inflammation, the initiation and maintenance of the inflammatory response involves cytokines or chemokines that are involved in the infiltration of various inflammatory cells, such as T cells or eosinophils, expressing skin lymphocyte-associated antigens, , Keratinocytes, fibroblasts, and the like. Chemokines are a family of cytokines and now more than 50 are known. They play a role in attracting appropriate immune cells to the site during the inflammatory reaction of the tissue. When the skin is stimulated by allergens or stimulants, the activated Langerhans cells migrate to the dermis, and T cells are activated by transferring the antigen to naive T cells that are not stimulated with the antigen. Memory T cells that have been exposed to an antigen once circulate along the blood vessels and migrate to the lesion area for quicker activation, leading to an effective immune response. T-cell mediated inflammatory diseases such as allergic contact dermatitis, psoriasis, and atopic dermatitis are representative skin diseases described by this mechanism.

Activated helper T cells, on the other hand, stimulate B cells and induce overproduction of immunoglobulin E. Immunoglobulin E causes inflammatory reactions such as irritation and erythema itch, and stimulates mast cells to activate another inflammatory pathway, COX (cyclooxygenase) signaling. COX-1 is intrinsically constant in tissues, whereas COX-2 is induced by inflammatory responses and is rapidly expressed in a short period of time by various stimuli. Increased expression of COX-2, a typical inflammatory factor, is known to affect the production of nitric oxide.

Currently, steroid preparations, topical immunosuppressants, topical antibiotics, and antihistamines are currently used as therapeutic agents for inflammatory skin diseases. However, when the drug preparation is used for a long period of time, Side effects such as palpitation may occur. In particular, when steroids are applied to a wide range of sites, medication can be systemically absorbed through the skin, which may interfere with growth of the child and systemic side effects such as osteoporosis can occur in adults.

Therefore, it is necessary to develop a composition derived from a natural substance, which has few side effects and is safe for the human body and is excellent in an inflammatory skin disease-improving effect.

It is an object of the present invention to provide a sea tangle extract for treating skin inflammatory diseases, which contains alginic acid and fucoidan as an active ingredient and which is beneficial to skin health and has an excellent effect of improving inflammatory skin diseases, and a composition containing the same.

The sea tangle extract and the composition containing the same for treating skin inflammatory disease according to the present invention are characterized in that alginic acid, fucoidan and a composition containing the same are extracted from sea tangle and at least one selected from sodium hyaluronate, butylene glycol, glycerin, squalane and jojoba oil .

Further, the present invention may contain alginic acid and fucoidan in an amount of 1 to 5% by weight.

In addition, the present invention may include 1.5 to 2.5% by weight of sodium hyaluronate, 2.5 to 3.5% by weight of butylene glycol, 2.5 to 3.5% by weight of glycerin, 3.5 to 4.5% by weight squalane and 3.5 to 4.5% by weight of jojoba oil have.

The present invention can also be used as a formulation selected from a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a cleansing, a foundation and a spray.

The kelp extract composition of the present invention has an advantage of being excellent in the therapeutic effect of skin inflammatory diseases, and being safe for cosmetic, pharmaceutical and food compositions because of no cytotoxicity and skin side effects.

1 is a graph showing collagen and elastin degrading enzyme inhibitory effects of vegetable mucus extracted from fresh-cut and anise of sea tangle extract of the present invention,
FIG. 2 is a graph showing data comparing the size of edema when treating kelp mucus by concentration,
FIG. 3 is a data graph comparing amounts of prostaglandins when treating kelp mucus by concentration,
4 is a graph showing the rate of inhibition of mast cells according to the morphology of alginic acid.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention, and it is to be understood by those skilled in the art that the present invention is not limited thereto It will be self-evident.

The present invention includes alginic acid and fucoidan compositions extracted from kelp.

The kelp grows 1.5 ~ 3m in length, and 2 ~ 4 year old leaves are spore generations. Apparent stem, leaf, and root are distinguished. In addition, kelp is rich in vitamins, making skin clear and firm, good anti-aging, anti-inflammatory and anti-allergic effects are also known.

Kelp contains alginic acid (Alginic Acid) and fucoidan (Fucoidan), both of which are active ingredients of various actions of kelp.

The alginic acid is a type of dietary fiber which activates intracellular metabolism to inhibit skin aging and make skin and hair healthy. Helps the formation of elastic cells such as collagen and elastin in the skin. It is rich in zinc called skin minerals, helping to eliminate skin troubles such as acne and rash.

Fucoidan is a very excellent natural moisturizing agent which seaweed secretes to protect itself from waves and sunlight. Recent studies have shown that fucoidan helps cell activity and prevents skin aging caused by UV rays. .

On the other hand, the kelp extract of the present invention is obtained by finely pulverizing and pulverizing the dried product, and adding water containing purified water of about 1 to 50 times (w / v), preferably about 1 to 5 times (w / v) To C4 lower alcohol or a mixed solvent thereof. Preferably, water, ethanol or a mixed solvent thereof is extracted by an extraction method such as stirring extraction, hot water extraction, cold extraction, reflux cooling extraction or ultrasonic extraction for 20 to 40 minutes , And the extract of the present invention can be obtained through drying and drying steps such as filtration, concentration under reduced pressure and freeze-drying.

[Examples and Comparative Examples]

A composition for treating skin inflammatory diseases comprising alginic acid and fucoidan components of the examples and comparative examples was prepared with the composition of the ingredients shown in the following Table 1. [

Throughout this specification, "%" used to denote the concentration of a particular substance is intended to include solids / solids (wt / wt), solid / liquid (wt / The liquid / liquid is (volume / volume).

ingredient Example Comparative Example Alginic acid and fucoidan One 0 Betaine One One Sodium hyaluronate 2 2 Butylene glycol 3 3 glycerin 3 3 Cetearyl alcohol 0.5 0.5 Stearic acid One One Polyglycerin-3 methylglucose distearate 1.6 1.6 Squalane 4 4 Jojoba oil 4 4 Glyceryl stearate 0.8 0.8 Dimethicone 0.5 0.5 Polyacrylate-13 & Polyisobutene & Polysorbate 20 0.7 0.7 incense a very small amount a very small amount Preservative a very small amount a very small amount Purified water Balance Balance

[ Experimental Example 1  : Evaluation test of collagen and elastin]

In order to confirm skin inflammation inhibition and improvement effect of kelp extract, collagen synthesis promotion effect and elastin production promoting effect were evaluated. The experimental method is as follows.

The cells were cultured in DMEM supplemented with 10% FBS and 1% antibiotic antimycotic in a 5% CO2 incubator at 37 ° C. HS68 cells were plated on a 24-well plate at 1 × 10 5 cells / well and cultured in a 5% CO2 incubator at 37 ° C for 24 h. The prepared fresh herb extract, anise extract and sea tangle extract were replaced with serum-free medium diluted in each sample, and cultured for 24 hours. The amount of collagen precursor C-terminal was measured by using Procollagen type I C-peptide EIA kit (Takara, Japan).

1 is a graph showing collagen and elastin degrading enzyme inhibitory effects of vegetable mucus extracted from fresh seaweed and anise of sea tangle extract of the present invention.

Referring to FIG. 1, chrysanthemum has antioxidative effects on chalcone and coumarin contained in yellow juice from leaves and stems, thereby preventing thrombosis, arteriosclerosis and cancer. Chalcone has excellent antimicrobial activity and coumarin has been shown to be effective against skin cancer and lung cancer.

Anise has a sweet and unique flavor, which has similar properties to female hormone estrogen, and is known to be effective in preventing and improving menopausal disorders, alleviating menstrual cramps, and relieving menstrual problems.

The results showed that the sample diluted with sea tangle extract had a high inhibition rate of collagenase and elastase degrading enzyme compared to the fresh mint extract, anise extract, and MMP-9 enzyme, which is deeply involved in the inflammatory reaction, It was confirmed that this was improved.

[ Experimental Example 2  : Evaluation test for anti-inflammatory effect]

The anti - inflammatory effect of kelp extract was evaluated to confirm skin inflammation inhibition and improvement.

To induce atopic dermatitis, 200 μl / mouse / day of 1% DNCB solution (Sigma-Aldrich, St. Louis, MO, USA) dissolved in a mixture of acetone and olive oil (3: 1) The whole dorsal skin of the mice was sensitized and sensitized by applying 0.5% DNCB solution for 3 weeks three times a week for boosting. The concentration of kelp extract in atopic dermatitis was increased to 10% and 20%, respectively.

In addition, prostaglandins are characterized by antacid retention, vasodilatation, smooth muscle stimulation and inflammatory mediators, which protect the cells from death, while inducing inflammation and pain. Thus, the serum was extracted every 7 days in the mice immunized with KLH, and the amount of furtha glandine was determined.

FIG. 2 is a graph of data comparing the size of edema when treated with kelp mucus by concentration, and FIG. 3 is a graph showing data comparing the amount of prostaglandin when treating kelp mucus by concentration.

Referring to FIGS. 2 and 3, it was found that the concentration of kelp mucus affects the anti-inflammatory effect.

[ Experimental Example 3  : Evaluation of antiallergic effect]

It was tested whether the alginic acid and fucoidan exhibited an antiallergic effect as the sea tangle extract ingredient of Example 1 of the present invention. And Comparative Example 1 in which alginic acid and fucoidan components were not added was used as comparative data.

The antiallergic effect can be confirmed by observing the mast cell count according to the presence or absence of the sea tangle extract component. In other words, if the allergen, which is the cause of allergy, becomes an antigen and enters our body, an antibody against the allergen (IgE) is generated and attached to the mast cell in the tissue, and then when the causative allergen comes in, IGE, which binds to mast cells, and histamine, serotonin, and other substances are released, resulting in the appearance of sneezing.

Therefore, the evaluation method for measuring mast cells is as follows.

The viability of HMC-1 cells was assayed by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide (MTT) assay. After 5 μl of HMC-1 cells (5 × 10 5 cells / ml) was treated with 1 μg / ml of the extract, 500 μl of the culture was treated with Phorbol 12-myrostate 13-acetate (PMA) ionophore A23187 for 8 hours. After stimulation, MTT solution (50 μl) was added and incubated at 37 ° C. overnight, and the crystals were dissolved in dimethyl sulfoxide (DMSO) and absorbance was measured at 540 nm. The cell viability was calculated assuming that the absorbance of the untreated cells treated with the cell extract and stimulant was 100%.

4 is a graph showing the rate of inhibition of mast cells according to the morphology of alginic acid.

Referring to FIG. 4, it was confirmed that anti-allergic effect was observed by observing inhibition of mast cells by the inclusion of alginic acid and fucoidan.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (4)

Wherein the composition comprises at least one member selected from the group consisting of sodium hyaluronate, butylene glycol, glycerin, squalane, and jojoba oil, and a composition containing the alginic acid, fucoidan and kelp extracted from sea tangle. Composition. The method according to claim 1,
Wherein the alginic acid and fucoidan are contained in an amount of 1 to 5% by weight, and a composition containing the same. The method according to claim 1,
Characterized in that it comprises 1.5 to 2.5% by weight of sodium hyaluronate, 2.5 to 3.5% by weight of butylene glycol, 2.5 to 3.5% by weight of glycerin, 3.5 to 4.5% by weight squalane and 3.5 to 4.5% by weight of jojoba oil Kelp extract for treating skin inflammatory diseases and compositions containing the same. 4. The method according to any one of claims 1 to 3,
A composition for treating skin inflammatory disease having a formulation selected from a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a cleansing, a foundation and a spray.

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