KR101533624B1 - A Cosmetic Composition Containing Extracts of Potentilla kleiniana Wight et Arnott Having antibacterial Activity - Google Patents

Abstract

The present invention relates to potentilla kleiniana Wight et Arnott) as an active ingredient. According to the present invention, there is provided a cosmetic composition excellent in antimicrobial effect, particularly anti-acne effect, when the active ingredient is Potentilla kleiniana Wight et Arnott which exhibits antibacterial activity against Propionibacterium acne .

Description

[0001] The present invention relates to a cosmetic composition comprising an extract of Rhodophyta sp. Having antibacterial activity,

The present invention relates to a potentilla kleiniana Wight et Arnott) extract and a cosmetic composition containing the same. More particularly, the present invention relates to an extract of Rhodophyta sp. Extract having antimicrobial activity against acne bacteria, particularly Propionibacterium acnes , and an anti-acne cosmetic composition containing the same.

Acne is a chronic inflammatory disease of the epidermis of the hair follicle, accompanied by various skin changes such as cotton (hardened sebaceous follicles in the follicle), papules (bulging skin lesions less than 1 cm in size), pus, blisters, nodules, , Which may result in concave scars or enlarged scars. Acne occurs mainly in the sebaceous glands attached to hair follicles, which are mainly hair follicles. Acne occurs more often during puberty in the 10-20 age group, during which the excess of male hormones causes the secretion of sebaceous glands to become vigorous and the epithelium of the hair follicle causes this keratosis (abnormal keratinization with incomplete and immature keratinization) It is known that acne is mainly caused by the formation of comedone (sebaceous follicle in the hair follicle), which is the basic lesion of acne. However, the exact cause of acne is not known, but one cause rather than a combination of several causes work.

Among these, acne rash caused by bacteria is well known to be caused by bacteria that reside in the hair follicle, especially Propionibacterium acnes . Propionibacterium acnes ) is a major cause of the trouble of eating and growing sebum. In a clean skin environment, the bacterium normally exists on the skin and does not cause any problems. However, when the sebaceous glands accumulate in the hair follicles, It grows. When this bacterium consumes the same fat (triglyceride) as sebaceous gland, it excretes a substance similar to butter, that is, a fatty acid. As a result, the balance of oil and pH on the surface of the skin is broken and the healthy skin which has maintained a weak acidity is alkalized, The function is deteriorated and a vicious cycle of the skin is caused.

In general, in order to prevent and alleviate the acne induced by Propionibacterium acnes , treatment with a drug or an oral drug is used. It is a medicine made by transforming topical antibiotics (clindamycin, erythromycin) and vitamin A, which have a direct sterilization effect against acne bacteria and prevent the production of free fatty acids. (Tretinoin, adrenaline), a powerful antimicrobial agent that reduces the number of bacterial populations, and has some anti-inflammatory and cotton-soluble acne ointment (benzoyl peroxide) . On the other hand, there are two major drugs to eat, antibiotics that act to sterilize acne bacillus and reduce inflammation, and retinoids, which are known as Roqueutan, which are made by modifying vitamin A. Retinoids are known to regulate sebum secretion by reducing sebaceous secretion. However, this medication is not only used to control sebum, but also to treat abnormal keratinization (abnormal keratinization with incomplete and immature keratinization), inflammation reaction, bacterial growth It helps to block all pathways of acne development. However, it has been pointed out that such treatment improves the immune system against the antimicrobial agents of the gastrointestinal bacterium in addition to the side effects such as gastrointestinal disorder, liver and kidney damage, and even giving pregnant women a birth defects.

Meanwhile, Potentilla kleiniana Wight et Arnott) is a perennial plant of the rosemary rosacea of the dicotyledonous plant and is also known as the waxy gabi. It is known that the herbs in the herb are regulated by blue fever, detoxification, fever, fever, boils, eczema, seawater, sore throat, social (snakebite), insect bites, malaria, Branches branch off from the armpit and grow to 20 ~ 60cm in height with the tip pointing upward. Root leaves are five foliage oval-shaped leaves gathered in fingers, with or without hairy hairs on the surface, with sawtooth on the edge. There are three stem leaves, and the petiole is shorter in the upper one.

Conventional patent technologies relating to anti-acne cosmetic compositions include Korea Patent Application No. 10-2008-0001359 entitled " Paecilomyces japonica ) is cultured in a medium containing mulberry leaf powder, characterized in that it comprises a culture solution obtained by culturing Propionibacterium acnes (acne causing bacteria) Republic of Korea Patent Application, including acnes) an anti-acne cosmetic composition "and, fermented soybeans, Portulaca Oleracea and willow extract and the second oregano, cypress, cinnamon extract and gold extracted by the threshold extracted by ultrasound extraction indicating the growth inhibiting effect on the 10 2009-0045957 " Antimicrobial, anti-acne, skin soothing, moisturizing and herbal medicine mixed extract having excellent sebum control activity " However, the technology for the anti - acne activity of garlic herb has not been disclosed.

Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of the cited papers and patent documents are incorporated herein by reference in their entirety to better understand the state of the art to which the present invention pertains and the content of the present invention.

The present inventors have made extensive efforts to develop a cosmetic composition having excellent antimicrobial activity, particularly antimicrobial activity against acne bacteria, using natural plant extracts. As a result, it has been found that when a cosmetic composition is provided using an extract of Garlic extract as an active ingredient, Propionibacterium acne , KCTC3314), and thus completed the present invention.

Accordingly, an object of the present invention is to provide a potentilla kleiniana Wight et Arnott) as an active ingredient.

According to one aspect of the invention there is provided garakji herbs (Potentilla kleiniana Wight et Arnott) extract as an active ingredient.

According to a preferred embodiment of the present invention, the herbal extract is selected from the group consisting of flowers, leaves, stems, fruits, seeds, roots and outposts of leaves, stems, flowers and roots And more preferably at least one species of extract. The antimicrobial effect of the extract is not limited to a specific site, but may be more preferable in terms of extraction efficiency and antimicrobial effect when extracted from a plant including leaves, stems, and roots.

According to a preferred embodiment of the present invention, the extract is extracted with at least one selected from the group consisting of water, methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol and glycerin Which is an extract extracted as a solvent. When extracting with water, it is preferable to perform hot water extraction at 50-120 ° C for 1-10 hours, and when it is extracted using an organic solvent, it is preferably extracted by immersion in an organic solvent at 5-40 ° C for 1-5 days desirable. Although the weight of the extraction solvent is not particularly limited, the extract can not be extracted sufficiently if it is used in an excessively small amount relative to the weight of the extract, and when it is used in an excessive amount, the extraction solvent is removed, . If hot water extraction is required, it is necessary to include the sample sufficiently and to perform extraction in a warmed state in order to increase the extraction efficiency. At this time, if the temperature is too low, it is difficult to sufficiently take advantage of the heating and it is necessary to extract a lot of time, and if the temperature is too high, the decomposition of the useful substance may occur. In the case of organic solvent extraction, it can be extracted at room temperature, and the sample is immersed in a sufficient extraction solvent for about 3 days to ensure sufficient extraction. If the extraction is not sufficient, To increase the extraction amount.

According to a preferred embodiment of the present invention, the extract of Rhizoctonia sp. Is characterized by having an antimicrobial activity against an acne bacterium. Especially, it has an antimicrobial activity against Propionibacterium acne . In the present invention, it is very important that the extract of Rhodophyta spp. Exhibits antibacterial activity against acne bacteria. Among the various bacteria that proliferate on the skin, the extracts of Rhizoctrion spp. Did not show significant activity against Staphylococcus aureus, Escherichia coli, Candida and Streptococcus, but the antimicrobial activity against acromycin was very good (see Experimental Examples below) The inclusion of a herbal extract as an active ingredient has the meaning that it is possible to provide a cosmetic composition effective for inhibiting acne bacterium.

According to a preferred embodiment of the present invention, the extract of the extract has a minimum inhibitory concentration (MIC) of 100 to 1000 ppm against acne bacteria. The minimum inhibitory concentration means the minimum concentration at which the antimicrobial activity is inhibited, and the lower the MIC value, the more effective the antimicrobial effect is at low concentration. In the case of the extract of the present invention, the minimum inhibitory concentration is preferably 100 to 1000 ppm, that is, 0.01 to 0.1%, more preferably 300 to 700 ppm, and most preferably 400 to 500 ppm. That is, the extract of Rhizoctonia sp. Is characterized by having antimicrobial activity even at a low concentration, and more preferably, excellent antifungal activity.

According to a preferred embodiment of the present invention, the extract of Zygosporangia herbaceousum is contained in an amount of 0.01 to 30% by weight based on the total weight of the cosmetic composition. The content range of the extract in the cosmetic composition of the present invention is important. When the extract is less than 0.01% by weight based on the total weight of the antimicrobial active cosmetic composition, the antimicrobial activity can not be sufficiently obtained and the function as a cosmetic for alleviating acne is inferior. When it exceeds 30% by weight, There is a problem of safety due to the above.

According to a preferred embodiment of the present invention, the antibacterial active cosmetic composition may contain other ingredients than the above-mentioned extract. At least one auxiliary component selected from the group consisting of glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin, Further comprising at least one component selected from the group consisting of butylene glycol, citric acid, panthenol and allantoin, wherein the at least one ingredient is selected from the group consisting of glycerin, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, squalane and sodium citrate, Most preferably glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin.

According to a preferred embodiment of the present invention, since the cosmetic composition of the present invention is basically applied to the skin, it can be provided with reference to the cosmetic composition of the related art, for example, a solution, a suspension, an emulsion, a paste, But are not limited to, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

Garakji herbs of the present invention (Potentilla kleiniana Wight et Arnott) extract exhibits antimicrobial activity against Propionibacterium acne . Therefore, the cosmetic composition of the present invention comprising the extract as an active ingredient is excellent in antibacterial effect, especially anti-acne effect.

FIG. 1 is a photograph showing the results of growth-inhibition assay for Staphyrococcus aureus in the extract of the extract of Rhodiola japonica.
FIG. 2 is a photograph showing the results of a growth-arresting assay for the Escherichia coli strain of the extract of Rhizoctonia sp.
Fig. 3 is a photograph showing the results of growth-inhibition assay for Candida albicans in the extract of Rhizoctonia sp .
Fig. 4 is a photograph showing the results of the growth-inhibition assay for the Pityrosporum ovale strain of the extract of Rhizoctonia sp.
FIG. 5 is a photograph showing the results of growth-inhibition assay for Propionibacterium acne of Rhodiola sp.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not to be construed as limiting the scope of the present invention. It will be self-evident.

Example

Throughout this specification, the percentages used to denote the concentration of a particular substance, unless otherwise noted, are as follows: solid / solid is (weight / weight), solid / liquid is (weight / volume) The liquid is (vol / vol)%.

Example  1. Garlic Herb Extract preparation

1-1. 99.5% methanol extract

The extract solution was filtered with a mesh of 380 mesh and filtered through a 0.45 μm membrane filter. The filtrate was filtered through a membrane filter of 380 mesh (Mesh filter) Lt; / RTI > The filtrate was concentrated under reduced pressure at 40 ° C in a distillation apparatus equipped with a cooling condenser, evaporated under reduced pressure and then freeze-dried to finally obtain 0.6353 g (dry weight) of a 99.5% methanol extract of the extract.

1-2. 94.0% ethanol extract

The extract solution was filtered with a mesh of 380 mesh and filtered through a 0.45 μm membrane filter. The filtrate was filtered through a filter with a mesh of 380 mesh, and the extract was washed three times with distilled water and 94.0% ethanol at a weight ratio of 1:70, Lt; / RTI > The filtrate was concentrated under reduced pressure at 40 ° C in a distillation apparatus equipped with a cooling condenser, evaporated under reduced pressure and then freeze-dried to finally obtain 0.5383 g (dry weight) of a 94.0% ethanol extract of the extract.

1-3. 70.0% ethanol extract

The extract was filtered with a mesh of 380 mesh and filtered through a 0.45 μm membrane filter. The filtrate was filtered through a filter with a mesh of 380 mesh, and the extract was washed three times with distilled water and 70.0% ethanol in a weight ratio of 1:70, Lt; / RTI > The filtrate was concentrated under reduced pressure at 40 ° C in a distillation apparatus equipped with a cooling condenser, evaporated under reduced pressure and then freeze-dried to finally obtain 0.5672 g (dry weight) of 70.0% ethanol extract of the extract.

1-4. Heat number  extract

The garlic extract and distilled water were weighed in a weight ratio of 1:20 and boiled at 105 ° C for 2 hours in a hot water extractor equipped with a cooling condenser and cooled to room temperature. The initial extract was filtered with a mesh of 380 mesh, filtered again with a 11 μm paper filter, and finally filtered once with a 0.45 μm membrane filter. Then, the mixture was concentrated under reduced pressure at 40 ° C in a distillation apparatus equipped with a cooling condenser, evaporated under reduced pressure and then freeze-dried to finally obtain 0.4637 g (dry weight) of hot-water extract of Sorghum extract.

Experimental Example  One. Growth station ( Paper Disc ) Measure

The antimicrobial activity of the extract of Rhodophyta herb extract against the common skin-growing bacteria was measured using the Rhodophyta extract of Example 1-1. Five species of staphylococcus aureus from Korea Microorganism Conservation Center aureus , KCTC1621), Escherichia coli coli, KCTC1039), Candida albicans (Candida albicans, KCTC7270), Petey Los Forum Ovalle (Pityrosporum ovale, KCCM11894) and propionic sludge tumefaciens acne (Propionibacterium acne , KCTC3314) were cultured and activated under each medium and each condition as shown in Table 1 below.

Skin-resident fungus Microbial strain Culture medium Culture conditions yellow
Staphylococcus Styrofoam caucus aureus
( Staphyrococcus aureus ) Tryptic strain 37 캜, 24 hr, Escherichia coli Escherichia coli
( Escherichia coli ) Trypticase strain 37 캜, 24 hr, Candida Candida albicans
( Candida albicans ) YM 25 캜, 24 hr, Dandruff Petite Ross Forum Ovalle
( Pityrosporum ovale ) Malt extract, Tween 40, Glycerol mono oleate, Ox-bile (Desicate) 37 캜, 72 hr, Acne bacteria Propionibacterium acnes
( Propionibacterium acne ) Reinforced clostridial 37 캜, 24 hr, anaerobic

Each of the above culture solutions was dispensed into Petri dishes in an amount of 100 μl each. Paper discs (ADVANTEC 8 mm paper disc) were then placed on the Petri dish. The extracts were then diluted with purified water to a concentration of 1%, 0.1%, and 0.01% Using the extract of Example 1-1) was loaded in each well. After the re - culture at each condition, the formed growth stones were measured. To confirm the obtained results, the above experiment was repeated three times. Triclosan (TRICLOSAN) was used as a positive control to compare and contrast the antimicrobial activity of the extracts of Rhizoctonia spp., And the results were as shown in Figs. 1 to 5 (see the drawing below).

As a result of the test for the growth of 5 strains, the extract of Gamjanggi herb showed no antimicrobial activity except for the causative agent of acne and showed high antimicrobial activity only against the causative agent of acne causative bacteria, Propionibacterium acnes (KCTC3314). As a result, the range of Clear zone of Petri dishes showing antimicrobial activity was measured, and the results of the growth-inhibition test for Propionibacterium acnes (KCTC3314) of the garlic herb were as shown in Table 2 below.

Positive Control
(TRICLOSAN) Garlic herb
10000ppm (1%) Garlic herb
1000 ppm (0.1%) Garlic herb
100 ppm (0.01%) Clear zone 3.30 ㎠ 4.52 cm 2 1.77 cm 2 0.79 cm 2

As a result of measuring the area of fresh seaweed, it was confirmed that the garlic extract showed better antimicrobial effect than TRICLOSAN which is a positive control at 10000ppm (1%) concentration.

Experimental Example  2. Minimum inhibitory concentration ( MIC ) Measure

In Experimental Example 1, the MIC (Minimal inhibitory concentration) test for acne causative bacteria was carried out by using the extract of Rhodophyta japonica extract of Example 1-1 in order to further demonstrate antibacterial activity against acne causing bacteria. For the experiment, 10 sterilized Reinforced Clostridial Broths were prepared and each 1% acne culturing solution was inoculated. The extract was added to each medium while varying the concentration of the extract obtained from Example 1-1. Then, all the medium was anaerobically incubated at 37 ° C under a CO ₂ condition for 72 hours, and then 100 μl each was added to Reinforced Clostridial Agar and anaerobically cultured to confirm the growth of the acne strain. In addition, the above experiments were repeated three times to confirm more objective results.

Experiment MIC concentration (%) Primary 0.040 Secondary 0.060 Third 0.040 Average 0.046

The MIC concentration of Acanthopanax senticosus extract obtained from the third measurement of MIC was as shown in Table 3 above. As a result, it was confirmed that the inhibitory effect on the acne strain activity of Rhizoctrion spp. Extract was shown at an average concentration of 0.046% (460ppm) minimum.

Experimental Example  3. Cytotoxicity test ( Neutral red assay )

In order to examine the cytotoxicity of the extract of Example 1-1, 200 占 퐇 of the human skin dermal fibroblast (CCD-986 sk) was dispensed into a 96-well plate at a concentration of 5 × 10 ⁴ cells / CO ₂ incubator for 24 hours. The medium was DMEM / high glucose (Dulbeco's Modified Eagle Medium, Hyclone) containing 10% bovine serum and 1% antibiotics. After culturing, the extract of Herba spp. Of Example 1-1 was treated for each concentration and cultured for 72 hours under the same culture conditions. After the culture was completed, the culture solution was removed, and the culture solution containing 5 mg / ml Neutral red reagent was added thereto, followed by incubation at 37 ° C for 2 hours. The culture medium was then removed, treated with 1% CaCl ₂ and formaldehyde, shaken at 400-500 rpm for 1 min, and then discarded. Then, 1% acetic acid and 50% ethanol were added to each well and shaken for 10 minutes. The results were measured by using a microplate reader at 570 nm / 655 nm. The results are shown in Table 4 Respectively.

sample Example __ Concentration (/ / ml) 100 75 50 25 10 5 One 0.1 0.01 Cell survival rate (%) 106.9 111.4 105.9 103.2 94.9 94.8 95.9 97.3 97.2

As a result of the cytotoxicity test, the extract of Rhodophyceae showed no cytotoxicity at a concentration of 100 ㎍ / ㎖ or less.

Prescription example  : Cosmetics  Of the composition Prescription example

Prescription example  1-1: Softening longevity

Examples of the formulation of the softening water in the cosmetic containing the extract of Example 1-1 are as follows.

ingredient Content (% by weight) Example (1-1) 0.1 One Polyoxyethylene hardened castor oil 0.5 2 Glycine 3.0 3 Dipotassium glycyrrhizate 0.1 4 1,3-butylene glycol 3.0 5 Sodium hyaruronate 0.1 6 ethanol 5.0 7 Antioxidant 0.1 8 Triethanolamine 0.1 9 EDTA 0.1 10 antiseptic Suitable amount 11 Purified water Balance

Sodium hyaruronate was dispersed in purified water with a propeller mixer (3000 rpm) to prepare a 1% solution. Raw materials 1 to 8 except for sodium hyaruronate were homogenized at 500 rpm in a water dissolving tank using a propeller mixer, completely dissolved by heating at 75 DEG C, and then cooled to room temperature. Then, the raw materials 9 to 11 were completely dissolved in a separate dissolution tank, and the mixture was put into the above-mentioned water dissolution tank and stirred and mixed. Example 1-1 was added thereto and thoroughly stirred and mixed to prepare a softening liquid containing Example 1-1.

Prescription example  1-2: Nourishing lotion

Examples of the prescription of the nutritional lotion among the cosmetics containing the extract of Example 1-1 are as follows.

ingredient Content (% by weight) Example (1-1) 0.1 One glycerin 7.0 2 Sorbitan stearate sucrose cocoate 2.0 3 Mineral oil 4.0 4 Trioctanoin 1.0 5 Stearic Acid 1.0 6 Glyceryl stearate 0.5 7 Sorbitan monostearate 1.0 8 Dimethicone 0.5 9 Antioxidant 0.3 10 Triethanolamine 0.1 11 Carbomer 0.2 12 EDTA 0.1 13 antiseptic Suitable amount 14 Purified water Balance

The carbomer was dispersed at 4000 rpm using a propeller mixer to prepare a 2% solution. Raw materials 1 to 6 were added to an aqueous dissolution tank and dispersed by stirring with a homomixer (2000 rpm) and then heated to 75 캜. The raw material 7-14 was added to the oily melting tank and dissolved by heating to 75 ° C. Then, the oil phase dissolved in the water dissolution tank was charged and emulsified (3000 rpm / 5 minutes) and then cooled to room temperature. Example 1-1 was added thereto and thoroughly stirred and mixed to prepare a nutrition lotion containing Example 1-1.

Prescription example  1-3: Essence

Examples of prescription of essence in cosmetics containing the extract of Example 1-1 are as follows.

ingredient Content (% by weight) Example (1-1) 0.1 One glycerin 5.0 2 1,3-butylene glycol 2.0 3 Polyethylene glycol 2.0 4 Carbomer 1.0 5 Sodium hyaruronate 0.1 6 Glycine 3.0 7 Polyacrylamide 2.0 8 Hydroxyethyl cellulose 0.2 9 ethanol 3.0 10 Antioxidant 0.3 11 Triethanolamine 0.1 12 Polyoxyethylene hardened castor oil 1.0 13 EDTA 0.1 14 antiseptic Suitable amount 15 Purified water Balance

Sodium hyaruronate and hydroxyethyl cellulose were dispersed in purified water in a propeller mixer (2000 rpm) to prepare a 1% solution. The carbomer was dispersed in purified water in a propeller mixer (4000 rpm) to prepare a 2% solution. On the other hand, raw materials 1 to 12 were added to an aqueous dissolution tank, stirred and dispersed with a homomixer (2000 rpm), and then heated to 75 캜. The warmed water phase was further cooled to room temperature. The ingredients 13 to 15 were completely dissolved in a separate dissolution tank, and the mixture was added to the above-mentioned water dissolution tank and mixed by stirring. Example 1-1 was added thereto and thoroughly stirred and mixed to prepare an essence containing Example 1-1.

Prescription example  1-4: Nourishing cream

An example of the formulation of the nutritional cream among the cosmetics containing the extract of Example 1-1 is as follows.

ingredient Content (% by weight) Example (1-1) 0.1 One 1,3-butylene glycol 3.0 2 glycerin 3.0 3 Hydrogenated lecithin 1.0 4 Octyldodecanol 3.0 5 Trioctanoin 2.0 6 Stearic Acid 1.5 7 Cetostearyl alcohol 2.0 8 Polysorbate 60 1.5 9 Sorbitan sesquioleate 2.0 10 Dimethicone 3.0 11 Antioxidant 0.3 12 Xanthan gum 0.2 13 Triethanolamine 0.1 14 EDTA 0.1 15 antiseptic Suitable amount 16 Purified water Balance

Raw materials 1 to 8 were put into an aqua yard, stirred with a homomixer (2000 rpm), dispersed and then heated to 75 캜. In addition, raw materials 9 to 16 were added to an oily dissolving tank and dissolved by heating to 80 ° C. The oil phase dissolved in the water-dissolving tank was charged and emulsified (3000 rpm / 10 minutes) and then cooled to room temperature. Example 1-1 was added thereto and thoroughly stirred and mixed to prepare a nutritive cream containing Example 1-1.

Prescription example  1-5: Pack

An example of the formulation of the pack in cosmetics containing the extract of Example 1-1 is as follows.

ingredient Content (% by weight) Example (1-1) 0.1 One glycerin 7.0 2 1,3-butylene glycol 3.0 3 Squalene 3.0 4 Dimethicone 3.0 5 Sodium hyaruronate 0.1 6 Glycine 2.0 7 Polyacrylamide 5.0 8 Antioxidant 0.3 9 Triethanolamine 0.1 10 EDTA 0.1 11 antiseptic Suitable amount 12 Purified water Balance

Ingredients 1 to 8 were completely dissolved in a separate dissolving tank and stirred and mixed. Then, Example 1-1 was added thereto, and the mixture was thoroughly stirred and mixed to prepare a pack containing Example 1-1.

Claims (10)

An antimicrobial active cosmetic composition for acne comprising an extract of Potentilla kleiniana Wight et Arnott as an active ingredient.
The method according to claim 1,
Wherein the extract is at least one kind of extract selected from the group consisting of flowers, leaves, stems, fruits, seeds, roots and outpastes of Rhizoctonia sp.
The method according to claim 1,
Wherein the extract is at least one selected from the group consisting of water, methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol and glycerin Wherein the antimicrobial active cosmetic composition is an antimicrobial active composition.
The method according to claim 1,
Wherein the extract is extracted by hot water extraction at 50-120 ° C for 1-10 hours or by immersion in an organic solvent at 5-40 ° C for 1-5 days.
delete The method according to claim 1,
Wherein the acne bacterium is Propionibacterium acne .
The method according to claim 1,
Wherein the extract has a minimum inhibitory concentration (MIC) of 100 to 1000 ppm against the acne bacterium.
The method according to claim 1,
Wherein the extract is in an amount of 0.01 to 30% by weight based on the total weight of the cosmetic composition.
The method according to claim 1,
Wherein the cosmetic composition further comprises at least one auxiliary ingredient selected from the group consisting of glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin Wherein the antimicrobial active cosmetic composition is an antimicrobial active composition.
The method according to claim 1,
The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, Or more of the total weight of the composition.

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