KR102158780B1 - Composition for improving skin comprising natural material extract - Google Patents

Abstract

The present invention relates to a cosmetic composition, a food composition, or a quasi-drug composition comprising an extract of Molsikja, Somok, Rhubarb, Osuyu, Opilcho, Hyangchunja or Kawaii as an active ingredient. Specifically, for antioxidants containing the extract as an active ingredient; For moisturizing the skin; For skin whitening; For improving skin troubles; For wrinkle improvement; For enhancing skin elasticity; Or it relates to a composition for skin regeneration.
The composition of the present invention is excellent in anti-glycosylation, melanin reduction, anti-inflammatory, collagen synthesis promotion, elastase activity inhibition, cell proliferation effect, antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity improvement, It can be usefully used for skin regeneration. Therefore, the composition of the present invention can be used as a cosmetic composition, food composition, and quasi-drug composition that is safe for the skin and has excellent skin condition improvement effect.

Description

Composition for improving skin comprising natural material extract

The present invention relates to a cosmetic composition, a food composition, or a quasi-drug composition comprising an extract of Molsikja, Somok, Rhubarb, Osuyu, Opilcho, Hyangchunja or Kawaii as an active ingredient.

Specifically, for antioxidants, which contain as an active ingredient an extract of Molsikja, Somok, rhubarb, Osuyu, Ohpilcho, Hyangchunja or Kawaii; For moisturizing the skin; For skin whitening; For improving skin troubles; For wrinkle improvement; For enhancing skin elasticity; Or it relates to a composition for skin regeneration.

Skin is increasingly damaged by increasing age or by ultraviolet rays, external contaminants and stress, and the function of protecting the skin from these factors is weakened, so that the ability to protect cells and proliferate decreases. In this case, our body starts the skin 　 regeneration 　 process for the damaged skin. Skin regeneration is the reaction of tissues to damage and is a process of tissue recovery, which lasts from 2 days to 3 weeks after the skin is damaged. During this period, fibroblasts accumulate collagen to fill the flaws of damaged skin, induce proliferation of fibroblasts, and reorganization of new cells and interstitial components occurs in the wound.

Recently, factors that threaten skin health such as an increase in the amount of ultraviolet rays due to environmental pollution, westernized lifestyle, and stress are increasing. They cause skin contamination, dryness, trouble, abnormal skin immune system, etc., and eventually cause dermatitis, roughness, and rapid aging.

In particular, there is a problem of promoting skin aging by increasing the concentration of reactive oxygen species in the body, and by attacking lipids, proteins, sugars, and DNA, which are cellular components, and causing peroxidation reactions. Accordingly, there is a need for development of a cosmetic composition having an antioxidant effect that suppresses the generation of reactive oxygen species and an anti-aging (skin aging prevention) effect through it.

Human skin color is determined by various factors such as the activity of melanocytes, distribution of blood vessels, thickness of the skin, and the presence or absence of pigments inside and outside the human body.In particular, enzymes such as tyrosinase are found in melanocytes. The black pigment called melanin is important. Melanin exists in the skin and plays an important role in protecting the body from ultraviolet rays, but when over-produced, it is known to promote pigmentation and skin aging and play a major role in inducing skin cancer.

In order to treat or alleviate excessive melanin pigmentation, substances with ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, and tyrosinase inhibitory activity have been previously used to treat or alleviate excessive melanin pigmentation. Were used in cosmetics or pharmaceuticals. However, their use is limited due to insufficient whitening effect, safety problems for the skin, and stability problems when blended in cosmetics.

On the other hand, cosmetics are used for skin protection, and various ingredients such as surfactants, preservatives, fragrances, sunscreens, and pigments are known to cause various problems such as inflammation, rashes, and swelling on the skin (Maibach. HI, Contact Dermatitis). , 6. 369-404, 1980). In addition, it is known that skin inflammation is also caused by ultraviolet rays from the sun, and sebum, sweat, fatty acids and proteins in cosmetic ingredients released from the body are decomposed into highly toxic substances by the skin flora. It may cause inflammation.

Inflammatory reaction is a physiological reaction to protect a living body from invasion of foreign substances such as bacteria and mechanical damage. It causes phenomena such as redness, tingling, burning, swelling, and tissue change, resulting in adjacent tissue cells and non-cellular components. It can also cause harmful damage to the field. Therefore, if the cause of inflammation does not disappear, as a result, chronic inflammation occurs, leading to more serious tissue damage. Accordingly, there is an urgent need to develop a cosmetic composition capable of initially suppressing skin troubles through an anti-inflammatory effect.

Collagen is a major matrix protein produced by fibroblasts of the skin. It maintains the mechanical firmness of the skin and the binding power of tissues, supports cell adhesion, and induces cell differentiation. Collagen decreases due to age increase and photoaging caused by UV irradiation, and collagen reduction is promoted by collagenase enzyme activity that degrades collagen. It is known that this is closely related to the formation of wrinkles in the skin.

In addition, elastin occupies a relatively small portion of the fibrous components in the dermis, and is known to have elasticity in the skin, lungs and blood vessels, and due to the atrophy of elastin, elastic fibrous pseudoxanthoma elasticum (PXE) When this occurs, it is known that skin elasticity decreases.

Currently, retinol, adenosine, and chlorella extracts are known as cosmetics for improving wrinkles or enhancing skin elasticity. Retinol is a substance that promotes collagen synthesis and inhibits elastase enzymes.However, it is unstable and is limited in usage due to safety issues such as irritation and rash when applied to the skin.Chlorella extract, etc., has insignificant moisturizing effect, so it is expected to be substantially effective. Is known to be difficult.

In addition, a non-enzymatic reaction occurs in which a simple sugar such as glucose or fructose forms a covalent bond with a protein or fat of a protein, which is called glycation. Glycosylated collagen prevents collagen from forming an appropriate structure in the extracellular matrix of the dermal layer, thereby losing the elasticity of the skin and promoting wrinkle formation. In addition, advanced glycation endproducts (AGEs) produced by saccharification are substances that have a brownish color, and as skin aging progresses, they accumulate on the dermis layer and the reflected light from the skin decreases. Accordingly, the amount of the final glycated product increases as skin aging progresses, and is considered one of the substances that gradually make the face look yellow and dull (Hiroshi, O. et al, Skin Research and Technology, 15, p. 496, 2009). .

As substances that inhibit saccharification, aminoguanidine, pyridoxamine, and aspirin are known, but these substances have limited amounts of use due to safety issues on the skin, or their effects are insignificant. There is a problem that cannot be expected.

Under the above background, under the above background, the present inventors study on substances that have excellent antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement, skin regeneration effect among natural resources. The present invention was completed by confirming that the seven extracts of natural materials have anti-glycosylation, melanin reduction, collagen synthesis promotion, elastase activity inhibition, and cell proliferation effects, respectively.

It is an object of the present invention to provide a cosmetic composition comprising an extract of Molsikja, Somok, Rhubarb, Osuyu, Opilcho, Hyangchunja or Kawaii as an active ingredient.

Specifically, for antioxidants containing the extract as an active ingredient; For moisturizing the skin; For skin whitening; For improving skin troubles; For wrinkle improvement; For enhancing skin elasticity; Or to provide a composition for skin regeneration.

Another object of the present invention is to provide a food composition comprising an extract of Molsikja, Somok, rhubarb, Osuyu, Opilcho, Hyangchunja or Kawaii as an active ingredient.

Another object of the present invention is to provide a quasi-drug composition comprising an extract of Molsikja, Somok, rhubarb, Osuyu, Opilcho, Hyangchunja or Kawaii as an active ingredient.

One aspect of the present invention provides a cosmetic composition comprising an extract of Molsikja, Somok, Rhubarb, Osuyu, Opilcho, Hyangchunja or Kawaii as an active ingredient.

In the present invention, " Cynips gallae - tinctoria , 沒食子)" is an insect hump created by spawning vulture hornets on young branches of a beech family. The outer surface is brown or olive green, with small bumps, and the quality is hard. Therefore, it is mainly used for dyes, leather tanning, hair dyes, and ink materials.

In the present invention, " Caesalpinia sappan , 蘇木)" is a legume evergreen tree distributed in tropical Asia such as India, Malaysia, and southern China, and is also called firewood, redwood, and redwood. The red-yellow wood part is used as a red dye, and the root is yellow dye. Specifically, it may mean a medicinal material using the heartwood (heartwood), which has been mainly used in the prescription of antibacterial agents, anti-inflammatory agents, and pain relievers in oriental medicine.

In the present invention, " Rheum officinale , 大黃" is a perennial plant belonging to the Mardiaceae family, distributed all over the world and grows in wetlands in mountain valleys. It has thick yellow roots, the stem is rough, and the inside is hollow, and the height of the straight main stem reaches 1m. Specifically, it may mean a medicinal material made using the rhizome of medicinal rhubarb ( Rheum officinale Baillon), which has been mainly used in the treatment of constipation, nosebleeds, diuresis, and edema in oriental medicine.

In the present invention, "Sewage ( Evodia rutaecarpa , 吳茱萸 )" is an unmatured fruit of the Sanchoaceae plant, Osuyu , and has been mainly used in the treatment of vomiting, headache, stomatitis, toothache, etc. by warming the stomach and stopping pain in oriental medicine. .

In the present invention, "Opilcho ( Potentilla kleiniana )" is a perennial plant that lies on the ground and extends in all directions, and is also called as Garakjinamul , Saham , Weft , Geoga , Oseongcho , etc. Fine hairs lie on the whole body, and several stems split from the base are about 50cm. Because of its efficacy in antipyretic, antitussive, detoxification, and small bells, it has been used as an antipyretic, cough, and sore throat in oriental medicine.

In the present invention, "Hyangchunja ( Toona sinensis , 香椿子)" means the fruit of the oak tree, and ripened dark brown with an oval capsule of 2.5cm in length from September to October. In oriental medicine, it has been used for the treatment of colds and rheumatoid arthritis.

In the present invention, " Piper methysticum , kava" is a shrub of the pepper genus found in Polynesia, Melanesia and Micronesia, etc., Awa (Hawaii), Ava (Samoa), Yagona (Fiji), Sakau (Pompei) , Hippopotamus, or kabakaba. Specifically, it may refer to a root portion, and carbaractone extracted from the root portion is known to have sedation, sleep induction, and anesthetic effects.

In the present invention, the "extract" refers to an extract obtained by extraction treatment of Molysikja, Somok, Rhubarb, Osuyu, Opilcho, Hyangchunja or Kawa, a dilution or concentrate of the extract, a dried product obtained by drying the extract, and of the extract. It includes the extract of all formulations that can be formed using the extract itself and the extract, such as a crude product, a purified product, or a mixture thereof. The extract may be extracted from natural, hybrid, or variety plants of Molysikja, Somok, Rhubarb, Osuyu, Opilcho, Hyangchunja, or Kawa, and may be extracted from plant tissue cultures.

In the present invention, a method for extracting the extract of Molsikja, Somok, Rhubarb, Osuyu, Opilcho, Hyangchunja or Kawaii is not particularly limited, and can be extracted according to a method commonly used in the art. It includes a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, etc., and these may be performed alone or in combination of two or more methods, but are not limited thereto.

The type of extraction solvent used for extraction in the present invention is not particularly limited, and any solvent known in the art may be used. Water (or distilled water) as an extraction solvent; Lower alcohols having 1 to 4 carbon atoms such as methanol, ethanol, propyl alcohol, and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof may be used, but is not limited thereto. In addition, a solvent extract may be prepared by extracting one or more times using the solvent, and a dry extract obtained by freeze-drying or spray-drying after distilling the solvent extract under reduced pressure may be prepared.

In one embodiment of the present invention, after preparing the extracts of Molsikja, Somok, Rhubarb, Osuyu, Opilcho, Hyangchunja, and Kawaii using methanol, its skin improvement effect was confirmed.

In the present invention, the extract of oleracea, somok, rhubarb, oleum, opilcho, hyangchunja, or kawaii extract may be included in the form of a fraction thereof, a processed product thereof, animals and plants including the same, and an extract thereof.

In the present invention, "fraction" means a result obtained by performing fractionation in order to separate a specific component or a specific component group from a mixture containing several different constituents.

The fractionation method for obtaining the fraction in the present invention is not particularly limited, and may be performed according to a method commonly used in the art. As a non-limiting example of the fractionation method, there is a method of obtaining a fraction from the extract by treating an extract obtained by extracting Molysikja, Somok, Rhubarb, Osuyu, Opilcho, Hyangchunja or Kawa, but is not limited thereto. Does not.

The kind of solvent used for the fractionation is not particularly limited, and any solvent known in the art may be used. Polar solvents such as water and alcohol as the fractionation solvent; Non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane may be used, and these may be used alone or in combination of two or more, but are not limited thereto.

Specifically, the cosmetic composition of the present invention is for antioxidant; For moisturizing the skin; For skin whitening; For improving skin troubles; For wrinkle improvement; For enhancing skin elasticity; Or it may be for skin regeneration.

In the present invention, "antioxidation" means inhibiting cellular oxidation caused by highly reactive free radicals or reactive oxygen species according to oxidative stress caused by intracellular metabolism or the influence of ultraviolet rays. And, it includes removing free radicals or reactive oxygen species, thereby reducing cell damage.

In the present invention, "skin moisturizing" means to increase the moisture sensation on the skin and maintain a moist state. Increasing the skin moisturizing effect can have a beneficial effect on improving skin wrinkles and increasing elasticity.

In the present invention, "skin whitening" means to lighten skin tone by inhibiting the synthesis of melanin pigments, and to improve skin hyperpigmentation such as spots and freckles caused by ultraviolet rays, hormones, or genetics. This whitening effect can be achieved, for example, by reducing the total amount of melanin pigment, but the present invention is not limited to this mechanism of action.

In the present invention, "improving skin troubles" means to inhibit or inhibit the generation of skin troubles, or alleviate already generated troubles, and can be achieved by anti-inflammatory, but the present invention is a It is not limited.

The skin trouble refers to a skin disease that may be caused by inflammation caused by excessive nitric oxide production in macrophages. Any skin diseases related to inflammation are included regardless of their type, and the skin diseases related to inflammation include erythema, acid burns, blistering skin diseases, lichen shape triggered by atopic dermatitis, psoriasis, radiation, chemicals, burns, etc. Type disease, itching due to allergies, seborrheic eczema, rose acne, pemphigus vulgaris, polymorphic exudative erythema, nodular erythema, balanitis, vulvitis, inflammatory hair loss such as alopecia areata, skin T-cell lymphoma, etc. , Skin rash, acne, pimples, rosacea (red nose) may be, but is not limited thereto.

The anti-inflammatory is meant to suppress the inflammation caused in the skin, which can be achieved by suppressing excessive nitric oxide (NO) production. In particular, the composition of the present invention may have an effect of improving skin troubles such as acne through an anti-inflammatory effect.

In the present invention, "wrinkle improvement" means suppressing or inhibiting the generation of wrinkles on the skin, or alleviating wrinkles that have already been created.

In the present invention, "improving skin elasticity" means reducing the degree of sagging or sagging of the skin.

In the present invention, "skin regeneration" means that skin tissue is recovered against damage caused by external and internal causes of the skin. The damage caused by the external cause may include ultraviolet rays, external pollutants, wounds, and trauma, and the damage caused by the internal cause may include, but is not limited to, stress.

Specifically, the composition of the present invention may have anti-glycosylation, melanin reduction, anti-inflammatory, collagen synthesis promotion, elastase activity inhibition, and cell proliferation effects. In an embodiment of the present invention, the extracts of Molsikja, Somok, Rhubarb, Osuyu, Opilcho, Hyangchunja, or Kawaii extracts have antioxidant and anti-glycosylation effects, melanin production inhibitory effects, NO production inhibitory effects, type I collagen Synthesis promoting effect, elastase activity inhibitory effect, cell proliferation effect (Tables 1 to 7), useful for antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement and/or skin regeneration It was confirmed that it can be used.

The cosmetic composition of the present invention is a solution, external ointment, cream, foam, nutrient lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil, suspension, emulsion It can be prepared in a formulation selected from the group consisting of liquid, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch, and spray, but is not limited thereto. .

In addition, the cosmetic composition of the present invention may additionally include one or more cosmetically acceptable carriers formulated in general skin cosmetics, and as common ingredients, for example, oil, water, surfactant, moisturizer, lower alcohol, A thickener, a chelating agent, a colorant, a preservative, a fragrance, and the like may be appropriately blended, but are not limited thereto. The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures thereof may be used, but are not limited thereto.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or mixtures thereof may be used as a carrier component, and in particular, in the case of a spray, additional chloro Propellants such as fluorohydrocarbon, propane/butane or dimethyl ether may be included, but are not limited thereto.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil may be used, and in particular, cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used. However, it is not limited thereto.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used, but are not limited thereto.

When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable oil, glycerol, sugar, etc. may be used as a carrier component. However, it is not limited thereto.

Another aspect of the present invention provides a food composition comprising an extract of Molsikja, Somok, rhubarb, Osuyu, Opilcho, Hyangchunja or Kawaii as an active ingredient.

Specifically, the food composition of the present invention is for antioxidant; For moisturizing the skin; For skin whitening; For improving skin troubles; For wrinkle improvement; For enhancing skin elasticity; Or it may be for skin regeneration.

Molshikja, Somok, Rhubarb, Osuyu, Ohpilcho, Hyangchunja, Kawa, antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement, and skin regeneration are as described above.

The food composition may be used in the form of a health functional food, but is not limited thereto. In addition, the food composition of the present invention may include food additives that are acceptable in terms of food in addition to the extract of molsikja, somok, rhubarb, sorghum, pilcho, hyangchunja or kawaii.

In the present invention, the term "food supplementary additive" means a component that can be added auxiliary to food, and is added to the manufacture of health functional foods of each formulation, and can be appropriately selected and used by a person skilled in the art. Examples of food additives include various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavors and natural flavors, coloring agents and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners. , pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages, and the like, but are not limited thereto.

The food composition of the present invention may contain a health functional food. The health food refers to a food manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and pills using raw materials or ingredients having useful functions for the human body. Here, the term “functionality” means obtaining a useful effect for health purposes such as controlling nutrients or physiological effects on the structure and function of the human body. The health functional food of the present invention can be prepared by a method commonly used in the art, and at the time of manufacture, it can be prepared by adding raw materials and ingredients commonly added in the art. In addition, the formulation of the health functional food may be prepared without limitation as long as it is a formulation recognized as a health functional food. The food composition of the present invention can be prepared in various forms of formulation, and unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long time using food as a raw material, and is excellent in portability. Health functional foods can be consumed as supplements for antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement or skin regeneration effect.

There is no limitation on the form that the health functional food of the present invention can take, and may include all foods in the usual sense, and may be mixed with terms known in the art such as functional foods. In addition, the health functional food of the present invention can be prepared by mixing suitable other auxiliary ingredients and known additives that may be included in the food according to the choice of a person skilled in the art. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and There are vitamin complexes and the like, and can be prepared by adding the extract according to the present invention as a main component, such as juice, tea, jelly, and juice. It also includes foods used as feed for animals.

Another aspect of the present invention provides a quasi-drug composition comprising an extract of Molsikja, Somok, Rhubarb, Osuyu, Opilcho, Hyangchunja or Kawaii as an active ingredient.

Specifically, the quasi-drug composition of the present invention is for antioxidant; For moisturizing the skin; For skin whitening; For improving skin troubles; For wrinkle improvement; For enhancing skin elasticity; Or it may be for skin regeneration.

Molshikja, Somok, Rhubarb, Osuyu, Ohpilcho, Hyangchunja, Kawa, antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement, and skin regeneration are as described above.

The quasi-drug composition of the present invention may further include a pharmaceutically acceptable carrier, excipient, or diluent, if necessary, in addition to Molsikja, Somok, rhubarb, Osuyu, Opilcho, Hyangchunja or Kawa extract. The pharmaceutically acceptable carrier, excipient, or diluent is not limited as long as it does not impair the effects of the present invention, for example, fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, preservatives, etc. Can include.

Representative examples of the pharmaceutically acceptable carrier, excipient or diluent of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, gum acacia, alginate, calcium phosphate, calcium Carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil , Injectable esters, witepsol, macrogol, Tween 61, cacao butter, laurage, etc., but are not limited thereto.

The quasi-drug composition of the present invention may exemplify a disinfectant cleaner, a shower foam, an ointment, a wet tissue, a coating agent, etc., but is not limited thereto, and the formulation method, dosage, usage method, component, etc. of quasi-drugs are known in the art. It can be appropriately selected from the techniques of.

The cosmetic composition, food composition, and quasi-drug composition of the present invention may additionally contain one or more active ingredients exhibiting the same or similar functions. For example, antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement, and/or skin regeneration components known in the art may be included. Due to the above additional ingredients, the antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement and/or skin regeneration effect of the composition of the present invention may be further enhanced. The content range of the additional ingredients may be appropriately adjusted by a person skilled in the art in consideration of skin safety, ease of formulation, stability of active ingredients, and the like according to combination use.

Further, the present invention comprises the step of applying an extract of the oleander, somok, rhubarb, oleum, pilcho, hyangchunja or kawaii on the skin of an individual, antioxidant; Moisturizing the skin; Skin whitening; Improvement of skin troubles; wrinkle improvement; Increase skin elasticity; Or it provides a method of skin regeneration. The individual may include, without limitation, mammals, including mice, livestock, and humans.

The composition of the present invention is excellent in anti-glycosylation, melanin reduction, anti-inflammatory, collagen synthesis promotion, elastase activity inhibition, cell proliferation effect, antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity improvement, It can be usefully used for skin regeneration. Therefore, the composition of the present invention can be used as a cosmetic composition, food composition, and quasi-drug composition that is safe for the skin and has excellent skin condition improvement effect.

Hereinafter, the present invention will be described in more detail through examples. These examples are for explaining the present invention more specifically, and the scope of the present invention is not limited to these examples.

Example 1: Preparation of the extract

After drying and mincing the molasses, 100 g of dry weight was put into a flask and extracted with 3000 g of an extraction solvent (70% MeOH) at 70° C. for 3 hours. The extract was filtered through a filter having a pore size of 0.2 µm, concentrated, and then dissolved in distilled water at 1:10 to prepare an extract of Molasko.

Example 2: Preparation of botanical extract

After drying and mincing the chop, 100 g of dry weight was put into a flask and extracted with 3000 g of an extraction solvent (70% methanol) at 70° C. for 3 hours. The extract was filtered through a filter having a pore size of 0.2 μm, concentrated, and then dissolved in distilled water at 1:10 to prepare a somok extract.

Example 3: rhubarb Preparation of extract

After drying and mincing the rhubarb, 100 g of dry weight was put into a flask and extracted with 3000 g of an extraction solvent (70% methanol) at 70° C. for 3 hours. The extract was filtered through a filter having a pore size of 0.2 μm, concentrated, and then dissolved in distilled water at 1:10 to prepare a rhubarb extract.

Example 4: Sewage Preparation of extract

After the sewage was well dried and minced, 100 g of dry weight was put into a flask and extracted with 3000 g of an extraction solvent (70% methanol) at 70° C. for 3 hours. The extract was filtered through a filter having a pore size of 0.2 μm, concentrated, and then dissolved in distilled water at 1:10 to prepare a sewage oil extract.

Example 5: Oh Pilcho Preparation of extract

After drying and mincing the Opilcho well, 100 g of dry weight was put into a flask and extracted with 3000 g of an extraction solvent (70% methanol) at 70° C. for 3 hours. The extract was filtered through a filter having a pore size of 0.2 μm, concentrated, and then dissolved in distilled water at 1:10 to prepare an extract of Opilcho.

Example 6: Hyangchunja Preparation of extract

Hyangchunja was well dried and minced, and then 100 g of dry weight was put into a flask and extracted with 3000 g of an extraction solvent (70% methanol) at 70° C. for 3 hours. The extract was filtered through a filter having a pore size of 0.2 μm, concentrated, and then dissolved in distilled water at 1:10 to prepare a Hyangchunja extract.

Example 7: Preparation of Kawa extract

After drying and slicing the Kawa well, 100 g of dry weight was put into a flask and extracted with 3000 g of an extraction solvent (70% methanol) at 70° C. for 3 hours. The extract was filtered through a filter having a pore size of 0.2 μm, concentrated, and then dissolved in distilled water at 1:10 to prepare a Kawa extract.

Experimental example 1: Antioxidant effect by scavenging free radicals

In order to check the antioxidant effect of the extracts prepared in Examples 1 to 7, the free radical scavenging ability was measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. (Blois, Nature 181, 1190, 1958). DPPH is a relatively stable free radical. When it exists in a radical state, it shows maximum absorption at 517 nm, and when the radicals are eliminated, it loses absorbance. DPPH was used by Sigma, and was dissolved in methanol at a concentration of 0.15 mM.

100 μl of each extract or vitamin C as a positive control was added to a 96-well plate, and 100 μl of DPPH solution was added thereto. After standing at room temperature for 30 minutes, absorbance at 517 nm was measured using a microplate reader (BioTek EL-340). The concentration (IC ₅₀ ) of the extract when the absorbance of the sample treated is half of the absorbance of the control group is summarized in Table 1 below. The experiment was performed three times and then the average value was calculated and shown.

sample IC ₅₀ (%w/v) Control 0.0025 Positive control group (vitamin C) 0.0005 Example 1 0.0011 Example 2 0.0014 Example 3 0.0018 Example 4 0.0009 Example 5 0.0009 Example 6 0.0012 Example 7 0.0018

As shown in Table 1, it was confirmed that each of Examples 1 to 7 had excellent antioxidant effects. Through this, it was found that all of the extracts of Molsikja, Somok, Rhubarb, Osuyu, Ohpilcho, Hyangchunja or Kawaii can be usefully used for antioxidant purposes.

Experimental example 2: Anti-glycosylation effect

In order to confirm the anti-glycosylation effect of the extracts prepared in Examples 1 to 7, the glycation inhibitory activity was measured using L-arginine and glucose.

First, 1M L-arginine and 1M glucose were dissolved in 1M phosphate buffer solution (pH 7.4), and the samples were diluted to 50ppm using 1M phosphate buffer solution. 1M L-arginine and 1M phosphate buffer were mixed in a ratio of 1:4, and then 80 μl was dispensed into a 96-well plate. A sample diluted to 50 ppm and 0.01M aminoguanidin to be used as a positive control were added to each of 100 μl. After mixing well, glucose diluted with 1M phosphate buffer was added so that the final concentration of glucose was 0.1M, and then reacted at 70°C for 4 hours. The degree of saccharification was measured by measuring the absorbance at 420 nm in a 96-well plate using a spectrophotometer.

The Glycation experiment group of the following formula was an experimental group in which 1M L-arginine and 1M glucose were added to induce saccharification.In order to measure the absorbance of the sample itself, 1M L-arginine and only the sample were added without glucose to measure the absorbance at 420 nm.

The glycated inhibition rate (%) was calculated using the following equation, and the average value was calculated after performing the experiment three times and summarized in Table 2.

sample Inhibition rate (%) Control (DMSO, 50ppm) - Positive control (Aminoguanidine, 55ppm) 54.89 Example 1 (50 ppm) 63.14 Example 1 (25 ppm) 36.31 Example 2 (50 ppm) 48.72 Example 2 (25 ppm) 29.76 Example 3 (50 ppm) 51.92 Example 3 (25 ppm) 39.82 Example 4 (50 ppm) 59.78 Example 4 (25 ppm) 41.70 Example 5 (50 ppm) 47.03 Example 5 (25 ppm) 27.63 Example 6 (50 ppm) 58.47 Example 6 (25 ppm) 36.92 Example 7 (50 ppm) 60.86 Example 7 (25 ppm) 38.57

As can be seen from the results of Table 2, the extracts of Examples 1 to 7 all exhibited excellent anti-glycosylation effect. Through this, it can be expected that a synergistic effect will appear by acting as an auxiliary role in applications such as skin moisturizing, skin whitening, wrinkle improvement, and skin elasticity enhancement.

Experimental example 3: Whitening effect due to inhibition of melanin production

Whitening effect by measuring the total amount of melanin by adding the extract to the culture medium of B-16 mouse melanoma cells according to the method described in Lotan R. et al. (Cancer Res. 40:3345-3350, 1980) Was confirmed. Before the experiment, toxicity was evaluated for the melanoma cells of mice, and a non-toxic concentration was selected to perform whitening evaluation. DMSO was used as a negative control, and arbutin was used as a positive control.

Specifically, the sample was added to the medium so that the final concentration was 100 ppm, and arbutin was added to the medium so that it became 100 ppm, and then melanoma cells were cultured for 3 days. Thereafter, the cells were treated with trypsin, removed from the culture vessel, centrifuged, and melanin was extracted. To the detached cells, 1 ml of sodium hydroxide solution (1N concentration) was added and boiled for 10 minutes to dissolve melanin, and absorbance was measured at 400 nm using a spectrophotometer to measure the amount of melanin produced.

The amount of melanin was measured by a method expressed by absorbance per unit cell number (1×10 ⁶ cells). The total amount of melanin relative to the control group was calculated as the melanin production inhibition rate (%), and after performing the experiment three times, the average value was calculated and summarized in Table 3.

sample Melanin production (abs) Inhibition rate (%) Control (DMSO, 10ppm) 0.33 - Positive control (Arbutin, 100ppm) 0.228 30.91 Example 1 (10 ppm) 0.241 26.97 Example 1 (1 ppm) 0.273 17.27 Example 2 (10 ppm) 0.248 24.85 Example 2 (1 ppm) 0.294 10.91 Example 3 (10 ppm) 0.257 22.12 Example 3 (1 ppm) 0.294 10.91 Example 4 (10 ppm) 0.261 20.91 Example 4 (1 ppm) 0.284 13.94 Example 5 (10 ppm) 0.259 21.52 Example 5 (1 ppm) 0.291 11.82 Example 6 (10 ppm) 0.239 27.58 Example 6 (1 ppm) 0.283 14.24 Example 7 (10 ppm) 0.252 23.64 Example 7 (1 ppm) 0.281 14.85

As can be seen from Table 3, the extracts of Examples 1 to 7 all exhibited excellent melanin production inhibitory effects even at low concentrations, and it was confirmed that the higher the concentration, the better the effect.

Experimental example 4: Anti-inflammatory effect by inhibiting NO production

In order to confirm the anti-inflammatory effect and skin trouble improvement effect, the ability to inhibit nitric oxide (NO) production was measured by the GRIESS method using RAW264.7 cell line (ATCC number: CRL-2278).

Specifically, RAW264.7 cells, which are mouse macrophages, were subcultured several times, and 3×10 ⁵ cells per well were placed in a 24-well plate and cultured for 24 hours. Then, it was replaced with a cell medium containing the sample at a final concentration of 10 ppm. At this time, the NO production inhibitor L-NG-monomethylarginine (L-NMMA) was treated together as a positive control and incubated for 30 minutes, and 1 ㎍ of LPS (Lipopolysaccharide) was treated as a stimulation source. And incubated for 24 hours. 100 µl of the supernatant was taken and transferred to a 96-well plate, 100 µl of the GRIESS solution was added at a time and reacted at room temperature for 10 minutes, and the absorbance at 540 nm was measured to confirm the inhibitory effect of NO generation.

The NO generation inhibition rate (%) was calculated using the following formula, and the average value was calculated after performing the experiment three times and summarized in Table 4.

sample NO production inhibition rate (%) Control (DMSO, 10ppm) - Positive control (L-NMMA, 10ppm) 36.76 Example 1 (1 ppm) 11.24 Example 1 (10 ppm) 18.30 Example 1 (100 ppm) 32.51 Example 2 (1 ppm) 1.54 Example 2 (10 ppm) 12.85 Example 2 (100 ppm) 22.29 Example 3 (1 ppm) 19.38 Example 3 (10 ppm) 20.37 Example 3 (100 ppm) 34.09 Example 4 (1 ppm) 16.30 Example 4 (10 ppm) 17.29 Example 4 (100 ppm) 23.51 Example 5 (1 ppm) 9.73 Example 5 (10 ppm) 18.51 Example 5 (100 ppm) 33.91 Example 6 (1 ppm) 16.88 Example 6 (10 ppm) 19.21 Example 6 (100 ppm) 21.48 Example 7 (1 ppm) 11.84 Example 7 (10 ppm) 19.50 Example 7 (100 ppm) 21.30

As can be seen from the results of Table 4, it was confirmed that the extracts of Examples 1 to 7 exhibit excellent anti-inflammatory activity as natural substances. Therefore, it can be used for improving skin troubles, and it can be expected to exhibit a synergistic effect by acting as an auxiliary role in skin whitening, wrinkle improvement, skin elasticity enhancement, and skin moisturization.

Experimental example 5: Effect of promoting the synthesis of type 1 collagen in fibroblasts derived from human body

The extracts of Examples 1 to 7 were added to the culture medium of human-derived fibroblasts to confirm the effect of promoting type 1 collagen synthesis at the cellular level. The synthesized collagen was quantified using the PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay KIT). In order to measure the amount of collagen synthesis, a sample was added to the fibroblast culture medium (DMEM medium) so that the final concentration was 10 ppm, cultured for 48 hours, and then the culture solution was taken, and type 1 collagen was synthesized at each concentration using the PICP EIA kit. The degree was measured at 450 nm using a spectrophotometer.

In order to compare the effect, the collagen synthesis degree was measured in the same manner for the sample to which the culture medium (negative control) and vitamin C (positive control) were added to the final concentration of 52.85 μg/ml of fibroblasts not treated with the sample.

The rate of increase in collagen production (%) was calculated as the ratio of the amount of collagen production relative to the negative control group, and the average value was calculated after performing the experiment three times and summarized in Table 5.

sample Type 1 collagen production (ng/ml) Increase rate (%) Negative control 150.2 - Positive control group (vitamin C) 246.8 64.3 Example 1 (1 ppm) 172.8 15.1 Example 1 (10 ppm) 231.7 54.3 Example 2 (1 ppm) 191.2 27.3 Example 2 (10 ppm) 223.3 48.7 Example 3 (1 ppm) 181.2 20.6 Example 3 (10 ppm) 243.6 62.18 Example 4 (1 ppm) 179.1 19.2 Example 4 (10 ppm) 223.7 48.9 Example 5 (1 ppm) 201.1 33.9 Example 5 (10 ppm) 254,9 69.7 Example 6 (1 ppm) 192.7 28.3 Example 6 (10 ppm) 257.5 71.4 Example 7 (1 ppm) 178.4 18.8 Example 7 (10 ppm) 229.4 52.7

As can be seen from Table 5, it was confirmed that the extracts of Examples 1 to 7 all promote collagen synthesis. In addition, it was found that the higher the concentration, the better the effect.

Experimental example 6: Elastase Inhibitory effect

The inhibitory effect of the activity of elastase, an enzyme that degrades elastin, was confirmed as follows. Elastase Elastase derived from human leukocytes was used, and MeOSuc-Ala-Ala-Pro-Val-pNA, a synthetic substrate, was used as a substrate. As a buffer solution, a 100 mM Tris (pH 7.5) solution was used. Elastase was finally used as 0.2 mU using a buffer solution. In addition, the synthetic substrate of elastase was diluted with a buffer solution to a final concentration of 0.5 mM after making a 100 mM solution using DMSO. At this time, the positive control group was set to contain quercetin, known as an elastase inhibitor, at a concentration of 10 ppm. The elastase inhibition candidate was added so that the final concentration was 10 ppm. The reaction was carried out in a 96-well plate, and the reaction was performed at room temperature for 20 minutes.

The absorbance was measured at 405 nm at 1 minute intervals using a spectrophotometer, and the slope of the absorbance against time was determined to determine the activity of the enzyme. The elastase inhibition rate (%) was calculated using the following formula, and the average value was calculated after performing the experiment three times and summarized in Table 6.

sample Enzyme activity Inhibition rate (%) Positive control (Quercetin, 10ppm) 3.5 66.35 Control (DMSO, 20ppm) 10.4 - Example 1 (10 ppm) 4.6 55.77 Example 1 (20 ppm) 2.7 74.04 Example 2 (10 ppm) 4.8 53.85 Example 2 (20 ppm) 4.1 60.58 Example 3 (10 ppm) 7.6 26.92 Example 3 (20 ppm) 4.5 56.73 Example 4 (10 ppm) 6.7 35.58 Example 4 (20 ppm) 5.2 50.00 Example 5 (10 ppm) 5.4 48.08 Example 5 (20 ppm) 4.2 59.62 Example 6 (10 ppm) 7.1 31.73 Example 6 (20 ppm) 4.6 55.77 Example 7 (10 ppm) 5.1 50.96 Example 7 (20 ppm) 3.6 65.38

As shown in the results of Table 6, it was confirmed that the extracts of Examples 1 to 7 all exhibit excellent inhibitory effects on elastase activity. In other words, it was found that it can be used for skin regeneration, wrinkle improvement, and skin elasticity enhancement.

Experimental example 7: Skin regeneration effect by cell proliferation

The cell proliferation effect of the extracts of Examples 1 to 7 was tested by the following method. Human fibroblast cells were inoculated and cultured in a 96-well plate (96 well plate, Corning), and then Examples 1 to 7 were applied at a concentration of 1% for 2 days, and the MTT test method was used. Cell proliferation was evaluated. More specifically, human fibroblasts were counted in a 24-well plate at 5×10 ³ per well using a hemocytometer and then dispensed. When cultured in DMEM containing 10% FBS for 48 hours and cultured for 40-50% of the surface area of the culture vessel, the sample was replaced with FBS-free DMEM and cultured for 24 hours. After incubation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, MTT ; Sigma M5655, USA] solution (2.5 mg/ml) was added 50 ul and incubated for an additional 3 hours. Thereafter, the entire cell culture was discarded, 200 ul of dimethyl sulfoxide (DMSO; Sigma D2650, USA) was treated and stirred for each well, and then 100 ul of 96 wells were taken and Enzyme-Linked Immunosorbent Assay. Absorbance was measured at 570 nm by (ELISA). Serum-free medium was used as a control group, and 1% serum was added to the control group. After performing the experiment three times, the average value was calculated and summarized in Table 7.

Type of sample Cell proliferation rate after 2 days (%) Control (serum-free medium) 100 Control group (serum 1%) 176 Example 1 (1%) 128 Example 2 (1%) 131 Example 3 (1%) 126 Example 4 (1%) 139 Example 5 (1%) 137 Example 6 (1%) 124 Example 7 (1%) 129

As shown in Table 7 above, it was found that all of Examples 1 to 7 had the effect of promoting cell proliferation and thus could be used for skin regeneration.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features thereof. In this regard, the embodiments described above are illustrative in all respects and should be understood as non-limiting. The scope of the present invention should be construed that all changes or modifications derived from the meaning and scope of the claims to be described later rather than the above detailed description and equivalent concepts are included in the scope of the present invention.

Claims (14)

A cosmetic composition comprising the extract of Mole, Somok, Rhubarb, Osuyu, Opilcho, Hyangchunja or Kawaii as an active ingredient.
The cosmetic composition of claim 1, wherein the composition is for antioxidants.
The cosmetic composition of claim 1, wherein the composition is for moisturizing the skin.
The cosmetic composition of claim 1, wherein the composition is for skin whitening.
The cosmetic composition of claim 1, wherein the composition is for improving skin problems.
The cosmetic composition of claim 1, wherein the composition is for wrinkle improvement.
The cosmetic composition of claim 1, wherein the composition is for enhancing skin elasticity.
The cosmetic composition of claim 1, wherein the composition is for skin regeneration.
The cosmetic composition according to any one of claims 1 to 8, wherein the composition has an anti-glycosylation, melanin reduction, collagen synthesis promotion, elastase activity inhibition or cell proliferation effect.
The method of any one of claims 1 to 8, wherein the composition is a solution, external ointment, cream, foam, nutrient lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid cleaning agent, bathing agent , Sunscreen cream, sun oil, suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch and spray selected from the group consisting of Having a formulation, a cosmetic composition.
A food composition comprising the extract of oleracea, somok, rhubarb, oleum, ohpilcho, hyangchunja or kawaii as an active ingredient.
The method of claim 11, wherein the composition is for antioxidant; For moisturizing the skin; For skin whitening; For improving skin troubles; For wrinkle improvement; For improving skin elasticity; Or for skin regeneration, food composition.
A quasi-drug composition comprising an extract of Molsikja, Somok, Rhubarb, Osuyu, Ohpilcho, Hyangchunja or Kawa as an active ingredient.
The method of claim 13, wherein the composition is for antioxidant; For moisturizing the skin; For skin whitening; For improving skin troubles; For wrinkle improvement; For enhancing skin elasticity; Or for skin regeneration, quasi-drug composition.