KR20200038115A - Composition for improving skin comprising natural material extract - Google Patents

Abstract

The present invention relates to a cosmetic composition, a food composition, or a quasi-drug composition, comprising an extract of Cynips gallae-tinctoria, Caesalpinia sappan, Rheum officinale, Evodia rutaecarpa, Potentilla kleiniana, Toona sinensis, or Piper methysticum, as an effective component. More specifically, the present invention relates to a composition comprising the extract as an effective component, for antioxidant effects; skin moisturization; skin lightening; alleviation of skin troubles; reduction of skin wrinkles; enhancement of skin elasticity; or skin regeneration. The composition of the present invention exhibits excellent effects in terms of anti-glycation, melanin reduction, anti-inflammatory effects, collagen synthesis acceleration, elastase activity inhibition, and cell proliferation. Therefore, the composition of the present invention can be beneficially used for antioxidant effects, skin moisturization, skin lightening, alleviation of skin troubles, reduction of skin wrinkles, enhancement of skin elasticity, and skin regeneration. Accordingly, the composition of the present invention may be used as a cosmetic composition, a food composition, and a quasi-drug composition that are remarkably effective in improving skin conditions, and are still safe to the skin.

Description

Composition for improving skin comprising natural material extract

The present invention relates to a cosmetic composition, a food composition or a quasi-drug composition comprising as an active ingredient a glutinous, bovine, rhubarb, osuyu, ohpilcho, hyangchunja or kawa extract.

Specifically, for anti-oxidation, including molybdenum, bovine, rhubarb, osuyu, ohpilcho, hyangchunja or Kawa extract as an active ingredient; For skin moisturizing; For skin whitening; For improving skin problems; Wrinkle improvement; For skin elasticity enhancement; Or it relates to a composition for skin regeneration.

Skin is increasingly damaged by age or by ultraviolet, external contaminants and stress, and the ability to protect the skin from these factors is weakened, resulting in poor cell protection and proliferation. In this case, the body begins the process of skin rejuvenation against damaged skin. Skin regeneration is the tissue's response to damage and is the process of tissue recovery, which lasts from 2 days to 3 weeks after the skin is damaged. At this time, fibroblasts accumulate collagen to fill the blemishes of damaged skin, induce proliferation of fibroblasts, and reconstruction of new cells and interstitial components in the wound site.

Recently, factors that threaten skin health such as an increase in the amount of ultraviolet rays due to environmental pollution, westernized lifestyle, and stress are increasing. These cause skin contamination, dryness, trouble, skin immunity system abnormalities, and eventually dermatitis, roughness, and rapid aging.

In particular, the concentration of free radicals in the body is increased, and these free radicals attack the cellular components of lipids, proteins, sugars, and DNA, thereby causing a peroxidation reaction, thereby promoting skin aging. Accordingly, there is a need to develop a cosmetic composition having an antioxidant effect that inhibits the generation of free radicals and an anti-aging (anti-aging) effect.

Human skin color is determined by a number of factors, including the activity of melanocytes, the distribution of blood vessels, the thickness of the skin, and the presence or absence of pigments inside and outside the human body.In particular, enzymes such as tyrosinase in melanocytes The black pigment called melanin produced by action is important. Melanin is present in the skin and plays an important function of protecting the body from ultraviolet rays, but when over-produced, it is known to promote pigmentation and skin aging and play a major role in inducing skin cancer.

Substances with ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, and tyrosinase inhibitory activity to treat or alleviate excessive melanin pigmentation These were used in combination with cosmetics and medicines. However, their use is limited due to insufficient whitening effects, safety problems for the skin, and stability problems when blended in cosmetics.

On the other hand, cosmetics are used to protect the skin, and various ingredients such as surfactants, preservatives, fragrances, sunscreens, and pigments are known to cause various problems such as inflammation, rash, and edema on the skin (Maibach. HI, Contact Dermatitis) , 6. 369-404, 1980). In addition, it is known that skin inflammation is caused by ultraviolet rays from the sun, and skin, as the sebum, sweat, fatty acids, and proteins in the cosmetic components discharged from the body are decomposed into highly toxic substances by the skin bacterial flora present on the skin. Inflammation may also be caused.

Inflammatory reaction is a physiological reaction to protect the living body from the invasion and mechanical damage of foreign substances such as bacteria, and causes phenomena such as redness, tingling, burning, swelling, and tissue changes. It can also cause harmful damage to the fields. Therefore, if the inflammation-inducing factor is not eliminated, chronic inflammation occurs as a result, resulting in even more serious tissue damage. Accordingly, there is an urgent need to develop a cosmetic composition capable of initially suppressing skin troubles through anti-inflammatory effects.

Collagen is a major matrix protein produced in fibroblasts of the skin, and maintains the mechanical firmness of the skin and the cohesion of tissues, supports cell adhesion, and induces cell differentiation. Collagen decreases with age and photoaging by ultraviolet irradiation, and collagen reduction is accelerated by collagenase enzyme activity that breaks down collagen. This is known to be closely related to the formation of wrinkles on the skin.

In addition, elastin occupies a relatively small portion of the fiber components in the dermis, and is known to have elasticity on skin, lungs, and blood vessels, and elastic fibrous gastric yellow tumor (PXE: pseudoxanthoma elasticum) due to atrophy of elastin. It is known that skin elasticity decreases when this occurs.

Currently, retinol, adenosine, chlorella extract, etc. are known as cosmetic agents for improving wrinkles or enhancing skin elasticity. Retinol is a substance that promotes collagen synthesis and inhibits the elastase enzyme, but is unstable and has limited use due to safety problems such as irritation and rash when applied to the skin. Is said to be difficult.

In addition, a non-enzymatic reaction in which a simple sugar such as glucose or fructose forms a covalent bond occurs in the protein or fat of the protein in the body, and this is called glycation. Glycosylated collagen prevents collagen from forming an appropriate structure in the extracellular matrix of the dermal layer, thereby losing skin elasticity and promoting wrinkle formation. In addition, advanced glycation end products (AGEs) produced by saccharification are brownish substances that accumulate on the top of the dermal layer as the skin ages and the reflected light reflected from the skin decreases. Accordingly, the final glycation end product increases as the skin aging progresses, and is gradually regarded as one of the substances that make the face yellow and gray (Hiroshi, O. et al, Skin Research and Technology, 15, p. 496, 2009). .

Aminoguanidine, Pyridoxamine, Aspirin, etc. are known as substances that inhibit glycation, but these substances have practical limitations due to limitations in use or insignificant effects due to safety problems with the skin. There is a problem that can not be expected.

Under the above background, under the above background, the present inventors have researched on substances having excellent antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement, and skin regeneration effect among natural resources. The present invention was completed by confirming that the extracts of 7 natural materials each have anti-glycosylation, melanin reduction, collagen synthesis promotion, elastase activity inhibition, and cell proliferation effects.

An object of the present invention is to provide a cosmetic composition comprising as an active ingredient, glutinous, bovine, rhubarb, Osuyu, Ohpilcho, Hyangchunja or Kawa extract.

Specifically, antioxidant for containing the extract as an active ingredient; For skin moisturizing; For skin whitening; For improving skin problems; Wrinkle improvement; For skin elasticity enhancement; Or to provide a composition for skin regeneration.

Another object of the present invention is to provide a food composition comprising as an active ingredient, glutinous, bovine, rhubarb, osuyu, ohpilcho, hyangchunja or Kawa extract.

Still another object of the present invention is to provide a quasi-drug composition comprising as an active ingredient a glutinous, bovine, rhubarb, osuyu, opilcho, hyangchunja or kawa extract.

One aspect of the present invention provides a cosmetic composition comprising as an active ingredient, glutinous, bovine, rhubarb, osuyu, ohpilcho, hyangchunja or Kawa extract.

In the present invention, " Cynops gallae - tinctoria (沒 食 子) "is a worm hump produced by the spawning of a beech beetle on a young branch of a beech family. The outer surface is brown or olive green with small bumps and hard quality. Therefore, it is mainly used for dyes, leather tanning, hair dyes, and ink materials.

In the present invention, " Caesalpinia sappan , 蘇木) "is a soybean and evergreen tree distributed in tropical Asia such as India, Malaysia, and southern China. It is also called firewood, red-eye, and crimson. The red-yellow wood part is used as a red dye, and the root is a yellow dye. Specifically, it may mean a medicine using a heartwood (心 材) of the tree, which has been mainly used for prescription of antibacterial, anti-inflammatory, and analgesic drugs in oriental medicine.

In the present invention, "Rhuum ( Rheum officinale , 大黃)" is a perennial plant belonging to the family Nodaceae, which is distributed all over the world and grows in wetlands of valleys. It has thick yellow roots, rough stems, hollow, straight stems up to 1 m high. Specifically, it may mean a medicine made by using rhizomes of Rheum officinale Baillon, which has been mainly used for treatment of constipation, nosebleeds, diuresis, and edema in oriental medicine.

In the present invention, "Sooyu ( Evodia rutaecarpa , 吳茱萸 ) "is the unripe fruit of the wild plant, the cypress, which has been used mainly in the treatment of vomiting, headache, stomatitis, toothache, etc. by warming the stomach, stopping pain, and controlling the circulation of the flag. .

In the present invention, "O Pilcho ( Potentilla kleiniana ) "is a perennial plant lying on the ground and extending in all directions. It is also called Garaknamul , Saham , Weft , Geoga , Ohseongcho , etc. The hairs are lying at an angle on the body, and several stems split at the base are about 50cm long. It has the effect of antipyretic, antitussive, detoxification, and edema, so it has been used as a medicine for antipyretics, coughing and sore throat in oriental medicine.

In the present invention, "Hyangchunja ( Toona sinensis , 香椿 子) "means the fruit of the oak tree, ripening to dark brown with an oval shaped 2.5cm long stem from September to October. It has been used for the treatment of colds, rheumatoid arthritis, etc.

In the present invention, "Kawa ( Piper methysticum , kava)" is a black pepper shrub found in Polynesia, Melanesia, and Micronesia, such as Awa (Hawaii), Ava (Samoa), Yagona (Fiji), Sakau (Ponpei). , Hamahama or Kaaba Kaba. Specifically, it may mean a root region, and it is known that the carabalactone extracted from the root region has a sedative, sleep-inducing, and anesthetic effect.

In the present invention, "extract" is an extract obtained by the extraction treatment of molybdenum, bovine, rhubarb, sorghum, opilcho, hyangchunja or kawa, dilution or concentrate of the extract, dried product obtained by drying the extract, the extract And extracts of all formulations that can be formed using the extract itself and the extract, such as a crude or purified product, or mixtures thereof. The extract may be extracted from natural, hybrid, or varietal plants of molluscs, cedar, rhubarb, oleander, opilcho, hyangchunja, or kawaii, and can also be extracted from plant tissue cultures.

In the present invention, the method of extracting the extract of molybdenum, bovine, rhubarb, osuyu, opilcho, hyangchunja or kawa is not particularly limited, and may be extracted according to a method commonly used in the art. Hot water extraction method, ultrasonic extraction method, filtration method, reflux extraction method, and the like, these may be performed alone or in combination of two or more methods, but are not limited thereto.

The type of the extraction solvent used for extraction in the present invention is not particularly limited, and any solvent known in the art may be used. The extraction solvent includes water (or distilled water); Lower alcohols having 1 to 4 carbon atoms such as methanol, ethanol, propyl alcohol, and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; And hydrocarbon-based solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture of these may be used, but is not limited thereto. In addition, a solvent extract may be prepared by extracting one or more times using the solvent, and a dry extract obtained by distilling the solvent extract under reduced pressure and then freeze-drying or spray drying may be prepared.

In an exemplary embodiment of the present invention, methanol was used to prepare a molar, bovine, rhubarb, osuyu, opilcho, hyangchunja, and kawaii extract, and then confirm its skin improvement efficacy.

In the present invention, the mole meal, bovine, rhubarb, osuyu, opilcho, hyangchunja or kawa extract may be included in the form of fractions thereof, processed products thereof, animals and plants containing them, and extracts thereof.

In the present invention, "fraction" means a result obtained by performing fractionation to separate a specific component or a specific component group from a mixture comprising various various constituents.

The fractionation method for obtaining a fraction in the present invention is not particularly limited, and may be performed according to a method commonly used in the art. Non-limiting examples of the fractionation method include, but are not limited to, a method of obtaining a fraction from the extract by treating a predetermined solvent to an extract obtained by extracting molybdenum, cedar, rhubarb, osuyu, opilcho, hyangchunja, or kawa Does not.

The type of solvent used in the fraction is not particularly limited, and any solvent known in the art may be used. The fractional solvent includes polar solvents such as water and alcohol; Non-polar solvents such as Hexane, Ethyl acetate, Chloroform, and Dichloromethane may be used, and these may be used alone or in combination of two or more, but are not limited thereto.

Specifically, the cosmetic composition of the present invention is for antioxidant; For skin moisturizing; For skin whitening; For improving skin problems; Wrinkle improvement; For skin elasticity enhancement; Or it may be for skin regeneration.

In the present invention, "antioxidation" means inhibiting cell oxidation by free radicals or reactive oxygen species, which are highly reactive according to oxidative stress due to the effects of metabolism or ultraviolet rays in cells. And removing free radicals or free radicals, thereby reducing cell damage.

In the present invention, "skin moisturizing" means to increase the feeling of moisture on the skin and maintain a moist state. Increasing the skin moisturizing effect can have a beneficial effect on improving the wrinkles and increasing the elasticity of the skin.

In the present invention, the term “skin whitening” refers to lightening the skin tone by inhibiting the synthesis of melanin pigments and improving skin hyperpigmentation, such as ultraviolet rays, hormones or oil-induced freckles and freckles. This whitening effect can be achieved, for example, by reducing the total amount of melanin pigment, but the present invention is not limited to this mechanism of action.

In the present invention, "improve skin trouble" refers to inhibiting or inhibiting the generation of troubles on the skin, or alleviating the problems that have already been created, and can be achieved by anti-inflammatory, but the present invention is based on this mechanism of action. It is not limited.

The skin trouble refers to a skin disease that may be caused by inflammation caused by excessive nitrogen monoxide production in macrophages. If it is a skin disease related to inflammation, it is included regardless of its type, and the skin disease related to inflammation includes atopic dermatitis, psoriasis, radiation, chemicals, burns, erythematous diseases, acid burns, blistering skin diseases, and thyroid patterns Types of diseases, including itching due to allergies, seborrheic eczema, rose acne, vulgar erythema, polymorphic exudative erythema, nodular erythema, inflammatory hair loss such as glansitis, vulvitis, alopecia areata, skin T-cell lymphoma, etc. , Skin rash, acne, rash, injection (red nose), but is not limited thereto.

The anti-inflammatory means inhibiting the inflammation developed in the skin, which can be achieved by inhibiting excessive nitric oxide (NO) production. In particular, the composition of the present invention may have an effect of improving skin trouble such as acne through the anti-inflammatory effect.

In the present invention, "wrinkle improvement" refers to suppressing or inhibiting the formation of wrinkles on the skin, or to relieve wrinkles already created.

In the present invention, "enhancing skin elasticity" It means to relieve the degree of sagging or sagging of the skin.

In the present invention, "skin regeneration" means that the skin tissue is restored against damage caused by external and internal causes of the skin. The damage caused by the external cause may include ultraviolet rays, external pollutants, wounds, trauma, etc., and the damage caused by the internal cause may include stress, but is not limited thereto.

Specifically, the composition of the present invention may have anti-glycosylation, melanin reduction, anti-inflammatory, collagen synthesis promotion, elastase activity inhibition, cell proliferation effect. In one embodiment of the present invention, anti-oxidant and anti-glycosylation effects, melanin production inhibitory effect, NO production inhibitory effect, type 1 collagen, type 1 collagen Synthetic promoting effect, elastase activity inhibitory effect, cell proliferation effect (Table 1 to Table 7), antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement and / or skin regeneration use It was confirmed that it can be used.

The cosmetic composition of the present invention is a solution, ointment for ointment, cream, foam, nutrient cosmetic, soft cosmetic, pack, soft water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil, suspension, emulsion Liquid, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch and spray can be prepared in a formulation selected from the group consisting of, but not limited to .

In addition, the cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers formulated in general skin cosmetics, and for example, oils, water, surfactants, moisturizers, lower alcohols as common ingredients, Thickeners, chelating agents, pigments, preservatives, fragrances, etc. may be appropriately blended, but are not limited thereto. The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures of these may be used, but are not limited thereto.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component, especially in the case of a spray, additional chloro Propellants such as fluorohydrocarbon, propane / butane or dimethyl ether, but are not limited thereto.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-Butylglycol oil can be used, in particular cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan. However, it is not limited thereto.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, suspensions such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar or trakant, etc. may be used, but are not limited thereto.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars, etc. may be used as carrier components. However, it is not limited thereto.

Another aspect of the present invention provides a food composition comprising as an active ingredient, glutinous, bovine, rhubarb, osuyu, ohpilcho, hyangchunja or Kawa extract.

Specifically, the food composition of the present invention is for antioxidant; For skin moisturizing; For skin whitening; For improving skin problems; Wrinkle improvement; For skin elasticity enhancement; Or it may be for skin regeneration.

Informal, bovine, rhubarb, osuyu, ohpilcho, hyangchunja, kawa, antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement, skin regeneration are as described above.

The food composition may be used in the form of a health functional food, but is not limited thereto. In addition, the food composition of the present invention may include food additives, food additives, in addition to edible, bovine, rhubarb, miso, pilcho, hyangchunja or kawa extract.

In the present invention, "food supplement additive" means a component that can be supplementally added to food, and is added to prepare a health functional food of each formulation, and can be appropriately selected and used by a person skilled in the art. Examples of food additives include flavors such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners , pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonic acid used in carbonated beverages, and the like.

The food composition of the present invention may include a health functional food. The healthy food refers to food prepared and processed in the form of tablets, capsules, powders, granules, liquids and pills using ingredients or ingredients having useful functionality for the human body. Here, the term "functionality" means obtaining a useful effect for health use, such as adjusting nutrients or physiological effects on the structure and function of the human body. The health functional food of the present invention can be manufactured by a method conventionally used in the art, and may be prepared by adding raw materials and ingredients commonly added in the art. In addition, the formulation of the health functional food can also be prepared without limitation as long as the formulation is recognized as a health functional food. The food composition of the present invention can be manufactured in various types of formulations, and has the advantage of not having side effects that may occur when taking the drug for a long time by using food as a raw material, unlike general medicines, and is excellent in portability. Health functional foods can be consumed as supplements for antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement or skin regeneration effect.

There is no limitation in the form that the health functional food of the present invention can take, it may include all foods in a common sense, and it is interchangeable with terms known in the art, such as functional food. In addition, the health functional food of the present invention can be prepared by mixing appropriate other auxiliary ingredients and known additives that may be included in the food according to the choice of those skilled in the art. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, dairy products including gums, ice cream, various soups, beverages, teas, drinks, alcoholic beverages, and There are vitamin complexes and the like, and can be prepared by adding the extract according to the present invention as a main component, and adding it to juice, tea, jelly, and juice. It also includes foods used as feed for animals.

Another aspect of the present invention provides a quasi-drug composition comprising as an active ingredient, glutinous, bovine, rhubarb, osuyu, opilcho, hyangchunja or Kawa extract.

Specifically, the quasi-drug composition of the present invention is for antioxidant; For skin moisturizing; For skin whitening; For improving skin problems; Wrinkle improvement; For skin elasticity enhancement; Or it may be for skin regeneration.

Informal, bovine, rhubarb, osuyu, ohpilcho, hyangchunja, kawa, antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement, skin regeneration are as described above.

The quasi-drug composition of the present invention may further include pharmaceutically acceptable carriers, excipients, or diluents, if necessary, in addition to edible, bovine, rhubarb, miso, filcho, or kawa extract. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as it does not impair the effects of the present invention, for example, fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, lubricants, sweeteners, fragrances, preservatives, etc. It can contain.

Representative examples of pharmaceutically acceptable carriers, excipients or diluents of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, acacia rubber, alginate, calcium phosphate, calcium Carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil , Injectable ester, witepsol, macrogol, tween 61, cacao butter, laurid, and the like, but is not limited thereto.

The quasi-drug composition of the present invention may be exemplified by disinfecting cleansers, shower foams, ointments, wipes, coatings, etc., but is not limited thereto, and formulation methods, dosages, methods of use, and components of quasi-drugs are generally known in the art. It can be appropriately selected from the technology.

The cosmetic composition, food composition, and quasi-drug composition of the present invention may further contain one or more active ingredients exhibiting the same or similar functions. For example, it may include antioxidants, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement and / or skin regeneration ingredients known in the art. Due to the additional ingredients, the antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement and / or skin regeneration effect of the composition of the present invention may be further enhanced. The content range of additional ingredients may be appropriately adjusted by a person skilled in the art in consideration of skin safety according to the combined use, ease of formulation, and stability of active ingredients.

Furthermore, the present invention comprises the step of applying a mosquito, bovine, rhubarb, Osu, Opilcho, Hyangchunja or Kawa extract to the skin of an individual, antioxidant; Skin moisturizing; Skin whitening; Skin trouble improvement; wrinkle improvement; Promoting skin elasticity; Or provide a method for skin regeneration. The individual may include, without limitation, mammals including rats, livestock, and humans.

The composition of the present invention has excellent anti-glycosylation, melanin reduction, anti-inflammatory, collagen synthesis promotion, elastase activity inhibition, cell proliferation effect, antioxidant, skin moisturizing, skin whitening, skin trouble improvement, wrinkle improvement, skin elasticity enhancement, It can be usefully used for skin regeneration. Therefore, the composition of the present invention can be used as a cosmetic composition, a food composition, and a quasi-drug composition that is safe for skin and has an excellent skin condition improving effect.

Hereinafter, the present invention will be described in more detail through examples. These examples are intended to illustrate the present invention more specifically, but the scope of the present invention is not limited to these examples.

Example  1: Preparation of extract from the molester

After drying and slicing well, the dry meal was put in a flask 100 g of dry weight and extracted at 70 ° C. for 3 hours with 3000 g of extraction solvent (70% MeOH). The extract was filtered through a filter having a pore size of 0.2 μm, concentrated, and dissolved in distilled water at 1:10 to prepare a molar extract.

Example  2: Preparation of pine tree extract

After drying and crushing the small well, 100 g of dry weight was placed in a flask and extracted with 3000 g of an extraction solvent (70% methanol) at 70 ° C. for 3 hours. The extract was filtered through a filter having a pore size of 0.2 μm, concentrated, and dissolved in distilled water at 1:10 to prepare a pine extract.

Example  3: rhubarb  Preparation of extract

After drying and rinsing the rhubarb well, 100 g of dry weight was placed in a flask and extracted with 3000 g of an extraction solvent (70% methanol) at 70 ° C for 3 hours. The extract was filtered through a filter having a pore size of 0.2 μm, concentrated, and dissolved in distilled water at 1:10 to prepare a rhubarb extract.

Example  4: Oh Suyu  Preparation of extract

After drying and rinsing the sewage oil well, 100 g of dry weight was placed in a flask and extracted with 3000 g of an extraction solvent (70% methanol) at 70 ° C. for 3 hours. The extract was filtered through a filter having a pore size of 0.2 μm, concentrated, and dissolved in distilled water at 1:10 to prepare a sewage oil extract.

Example  5: Oh Pilcho  Preparation of extract

After drying and rinsing off the well, 100 g of dry weight was placed in a flask and extracted with 3000 g of an extraction solvent (70% methanol) at 70 ° C for 3 hours. The extract was filtered through a filter having a pore size of 0.2 µm, concentrated, and dissolved in distilled water at 1:10 to prepare an Orfilacho extract.

Example  6: Hyangchunja  Preparation of extract

After drying and slicing Hyangchunja well, 100 g of dry weight was placed in a flask and extracted with 3000 g of an extraction solvent (70% methanol) at 70 ° C for 3 hours. The extract was filtered through a filter having a pore size of 0.2 μm, concentrated, and dissolved in distilled water at 1:10 to prepare a Hyangchunja extract.

Example  7: Preparation of Kawa extract

After drying and crushing the Kawase well, 100 g of dry weight was placed in a flask and extracted with 3000 g of an extraction solvent (70% methanol) at 70 ° C for 3 hours. The extract was filtered through a filter having a pore size of 0.2 μm, concentrated, and dissolved in distilled water at 1:10 to prepare a Kawa extract.

Experimental example  1: Antioxidant effect by free radical scavenging

To confirm the antioxidant effect of the extracts prepared in Examples 1 to 7, 1,1-diphenyl-2-picrylhydrazyl (1,1-diphenyl-2-picrylhydrazyl; DPPH) method to measure the free radical scavenging ability (Blois, Nature 181, 1190, 1958). DPPH is a relatively stable free radical that exhibits maximum absorption at 517 nm when present in a radical state and loses absorbance when the radical is eliminated. DPPH was used by Sigma, and dissolved in methanol at a concentration of 0.15 mM.

Vitamin C, an extract or a positive control, was added to each 96-well plate, and 100 μl of DPPH solution was added thereto. After standing at room temperature for 30 minutes, the absorbance at 517 nm was measured using a microplate reader (BioTek EL-340). The concentration of the extract (IC ₅₀ ) when the absorbance of the sample treated is half the absorbance of the control group is summarized in Table 1 below. The experiment was performed three times, and the average value was calculated and presented.

sample IC ₅₀ (% w / v) Control 0.0025 Positive Control (Vitamin C) 0.0005 Example 1 0.0011 Example 2 0.0014 Example 3 0.0018 Example 4 0.0009 Example 5 0.0009 Example 6 0.0012 Example 7 0.0018

As shown in Table 1, it was confirmed that each of Examples 1 to 7 had excellent antioxidant effects. Through this, it was found that all of the edible, bovine, rhubarb, osuyu, opilcho, hyangchunja or kawa extracts can be usefully used for antioxidant purposes.

Experimental example  2: Antiglycosylation  effect

In order to confirm the anti-glycosylation effect of the extracts prepared in Examples 1 to 7, L-arginine and glucose were used to measure glycosylation inhibitory activity.

First, 1M L-arginine and 1M glucose were dissolved in 1M phosphate buffer solution (pH 7.4), and samples were diluted to 50 ppm using 1M phosphate buffer solution. 1M L-arginine and 1M phosphate buffer solution were mixed at a ratio of 1: 4, and then 80 μl was dispensed in a 96-well plate. To this, 100 μl of each sample diluted with 50 ppm and 0.01 M aminoguanidin to be used as a positive control were added. After mixing well, glucose diluted with 1 M phosphate buffer solution was added so that the final concentration of glucose was 0.1 M, followed by reaction at 70 ° C. for 4 hours. The 96-well plate was measured for absorbance by measuring the absorbance at 420 nm using a spectrophotometer.

Glycation experimental group of the following formula is an experimental group inducing saccharification by adding 1M L-arginine and 1M glucose, and in order to measure the absorbance of the sample itself, absorbance was measured at 420 nm by adding only 1M L-arginine and a sample without adding glucose.

The glycation inhibition rate (%) was calculated using the following formula, and after performing the experiment three times, the average value was calculated and summarized in Table 2.

sample Inhibition rate (%) Control group (DMSO, 50ppm) - Aminoguanidine (55ppm) 54.89 Example 1 (50 ppm) 63.14 Example 1 (25 ppm) 36.31 Example 2 (50 ppm) 48.72 Example 2 (25 ppm) 29.76 Example 3 (50 ppm) 51.92 Example 3 (25 ppm) 39.82 Example 4 (50 ppm) 59.78 Example 4 (25 ppm) 41.70 Example 5 (50 ppm) 47.03 Example 5 (25 ppm) 27.63 Example 6 (50 ppm) 58.47 Example 6 (25 ppm) 36.92 Example 7 (50 ppm) 60.86 Example 7 (25 ppm) 38.57

As can be seen from the results of Table 2, the extracts of Examples 1 to 7 all exhibited excellent anti-glycosylation effects. Through this, it can be expected that a synergistic effect will be exhibited by acting as an auxiliary role in applications such as skin moisturizing, skin whitening, wrinkle improvement, and skin elasticity enhancement.

Experimental example  3: Whitening effect by suppressing melanin production

According to the method described in Lotan R. et al. (Cancer Res. 40: 3345-3350, 1980), the whitening effect is obtained by measuring the total amount of melanin by adding the extract to a culture medium of mouse melanoma cells (B-16 mouse melanoma cells). Was confirmed. Prior to the experiment, the whitening evaluation was performed by selecting the non-toxic concentration by evaluating the toxicity of the melanoma cells of the mouse. DMSO was used as a negative control, and albutin was used as a positive control.

Specifically, the sample was added to the medium so that the final concentration was 100 ppm, and arbutin was added to the medium to be 100 ppm, and then melanoma cells were cultured for 3 days. Thereafter, cells were trypsin-treated, separated from the culture vessel, centrifuged, and melanin was extracted. 1 ml of sodium hydroxide solution (1N concentration) was added to the detached cells and boiled for 10 minutes to dissolve melanin, and the absorbance was measured at 400 nm using a spectrophotometer to measure the amount of melanin produced.

The amount of melanin was measured by a method represented by absorbance per unit cell number (1 × 10 ⁶ cells). The total amount of melanin relative to the control group was calculated as the rate of inhibition of melanin production (%), and the average value was calculated after performing the experiment three times and summarized in Table 3.

sample Melanin production (abs) Inhibition rate (%) Control group (DMSO, 10ppm) 0.33 - Positive control (arbutin, 100 ppm) 0.228 30.91 Example 1 (10 ppm) 0.241 26.97 Example 1 (1 ppm) 0.273 17.27 Example 2 (10 ppm) 0.248 24.85 Example 2 (1 ppm) 0.294 10.91 Example 3 (10 ppm) 0.257 22.12 Example 3 (1 ppm) 0.294 10.91 Example 4 (10 ppm) 0.261 20.91 Example 4 (1 ppm) 0.284 13.94 Example 5 (10 ppm) 0.259 21.52 Example 5 (1 ppm) 0.291 11.82 Example 6 (10 ppm) 0.239 27.58 Example 6 (1 ppm) 0.283 14.24 Example 7 (10 ppm) 0.252 23.64 Example 7 (1 ppm) 0.281 14.85

As can be seen from Table 3, the extracts of Examples 1 to 7 all exhibited excellent melanin production inhibitory effect even at low concentrations, and it was confirmed that the higher the concentration, the better the effect.

Experimental example  4: Anti-inflammatory effect according to NO production inhibition

In order to confirm the anti-inflammatory effect and the improvement of skin trouble, the ability to inhibit nitric oxide (NO) production was measured by the GRIESS method using the RAW264.7 cell line (ATCC number: CRL-2278).

Specifically, RAW264.7 cells, which are macrophages of mice, were passaged several times, 3 × 10 ⁵ cells per well were placed in a 24-well plate, and cultured for 24 hours. Subsequently, the sample was replaced with a cell medium containing a sample at a concentration of 10 ppm. At this time, L-NG-monomethylarginine (L-NMMA), an inhibitor of NO production, was treated together as a positive control group and cultured for 30 minutes, and 1 μg of LPS (Lipopolysaccharide) was treated as a stimulator. And cultured for 24 hours. After 100 μl of the supernatant was transferred to a 96-well plate, 100 μl of the GRIESS solution was added and reacted for 10 minutes at room temperature, and the effect of inhibiting NO production was confirmed by measuring absorbance at 540 nm.

The inhibition rate of NO production (%) was calculated using the following formula, and the average value was calculated after performing the experiment three times and summarized in Table 4.

sample NO production inhibition rate (%) Control group (DMSO, 10ppm) - Positive control (L-NMMA, 10ppm) 36.76 Example 1 (1 ppm) 11.24 Example 1 (10 ppm) 18.30 Example 1 (100 ppm) 32.51 Example 2 (1 ppm) 1.54 Example 2 (10 ppm) 12.85 Example 2 (100 ppm) 22.29 Example 3 (1 ppm) 19.38 Example 3 (10 ppm) 20.37 Example 3 (100 ppm) 34.09 Example 4 (1 ppm) 16.30 Example 4 (10 ppm) 17.29 Example 4 (100 ppm) 23.51 Example 5 (1 ppm) 9.73 Example 5 (10 ppm) 18.51 Example 5 (100 ppm) 33.91 Example 6 (1 ppm) 16.88 Example 6 (10 ppm) 19.21 Example 6 (100 ppm) 21.48 Example 7 (1 ppm) 11.84 Example 7 (10 ppm) 19.50 Example 7 (100 ppm) 21.30

As can be seen from the results in Table 4, it was confirmed that the extracts of Examples 1 to 7 exhibit excellent anti-inflammatory activity as a natural material. Therefore, it can be used for skin trouble improvement, skin whitening, wrinkle improvement, skin elasticity enhancement, and skin moisturizing, it can be expected to show a synergistic effect by acting as an auxiliary role.

Experimental example  5: Effect of promoting the synthesis of type 1 collagen in fibroblasts derived from the human body

The extracts of Examples 1 to 7 were added to the culture medium of human-derived fibroblasts to confirm the effect of promoting collagen type 1 synthesis at the cellular level. The measured collagen was quantified using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay KIT). To measure the amount of collagen synthesis, the sample was added to the culture medium of fibroblasts (DMEM medium) so that the final concentration was 10 ppm, and then incubated for 48 hours, the culture solution was taken, and the collagen type 1 was synthesized at each concentration using the PICP EIA kit. The degree was measured at 450 nm using a spectrophotometer.

In order to compare the effects, the degree of collagen synthesis was measured in the same manner for the samples in which the culture medium (negative control) and vitamin C (positive control) of untreated fibroblasts were added to a final concentration of 52.85 µg / ml.

The increase rate of collagen production (%) was calculated as the ratio of the amount of collagen production relative to the negative control group. The average value was calculated after performing the experiment three times and summarized in Table 5.

sample Type 1 collagen production (ng / ml) Growth rate (%) Eumseong Control 150.2 - Positive Control (Vitamin C) 246.8 64.3 Example 1 (1 ppm) 172.8 15.1 Example 1 (10 ppm) 231.7 54.3 Example 2 (1 ppm) 191.2 27.3 Example 2 (10 ppm) 223.3 48.7 Example 3 (1 ppm) 181.2 20.6 Example 3 (10 ppm) 243.6 62.18 Example 4 (1 ppm) 179.1 19.2 Example 4 (10 ppm) 223.7 48.9 Example 5 (1 ppm) 201.1 33.9 Example 5 (10 ppm) 254,9 69.7 Example 6 (1 ppm) 192.7 28.3 Example 6 (10 ppm) 257.5 71.4 Example 7 (1 ppm) 178.4 18.8 Example 7 (10 ppm) 229.4 52.7

As can be seen from Table 5, it was confirmed that the extracts of Examples 1 to 7 all promote collagen synthesis. In addition, it was found that the higher the concentration, the better the effect.

Experimental example  6: Elastase  Active inhibitory effect

The effect of inhibiting the activity of Elastase, an enzyme that breaks down Elastin, was confirmed as follows. Elastase Elastase derived from human leukocyte cells was used, and a synthetic substrate, MeOSuc-Ala-Ala-Pro-Val-pNA, was used. A buffer solution of 100 mM Tris (pH 7.5) was used. Elastase was finally used 0.2 mU using a buffer solution. In addition, the synthetic substrate of elastase was diluted with a buffer solution to make a final concentration of 0.5mM after preparing a 100mM solution using DMSO. In this case, the positive control group was set to contain 10 ppm of quercetin, known as an elastase inhibitor. Elastase inhibition candidates were added to a final concentration of 10 ppm. The reaction was carried out in a 96-well plate and reacted at room temperature for 20 minutes.

The absorbance was measured at 405 nm at 1 minute intervals using a spectrophotometer, and the slope of the absorbance versus time was determined to determine the activity of the enzyme. The elastase inhibition rate (%) was calculated using the following formula, and the average value was calculated after performing the experiment three times and summarized in Table 6.

sample Enzyme activity Inhibition rate (%) Positive control (Quercetin, 10ppm) 3.5 66.35 Control group (DMSO, 20ppm) 10.4 - Example 1 (10 ppm) 4.6 55.77 Example 1 (20 ppm) 2.7 74.04 Example 2 (10 ppm) 4.8 53.85 Example 2 (20 ppm) 4.1 60.58 Example 3 (10 ppm) 7.6 26.92 Example 3 (20 ppm) 4.5 56.73 Example 4 (10 ppm) 6.7 35.58 Example 4 (20 ppm) 5.2 50.00 Example 5 (10 ppm) 5.4 48.08 Example 5 (20 ppm) 4.2 59.62 Example 6 (10 ppm) 7.1 31.73 Example 6 (20 ppm) 4.6 55.77 Example 7 (10 ppm) 5.1 50.96 Example 7 (20 ppm) 3.6 65.38

As shown in Table 6, it was confirmed that the extracts of Examples 1 to 7 were all excellent in inhibiting the elastase activity. That is, it was found that it can be used for skin regeneration, wrinkle improvement, and skin elasticity.

Experimental example  7: Skin regeneration effect by cell proliferation

The cell proliferation effect of the extracts of Examples 1 to 7 was tested by the following method. Human fibroblast cells (human fibroblast cells) were inoculated in a 96-well plate (96 well plate, Corning), cultured, and then applied to Examples 1 to 7 at a concentration of 1% for 2 days Cell proliferation was evaluated. More specifically, human fibroblasts were counted and distributed using a hemocytometer equal to 5 × 10 ³ cells per well in a 24-well plate. When cultured in DMEM containing 10% FBS for 48 hours, and incubated for 40-50% of the surface area of the culture vessel, the sample was replaced with FBS-free DMEM and further cultured for 24 hours. 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide after culture (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide, MTT ; Sigma M5655, USA] 50 ul of solution (2.5 mg / ml) was added and incubated for an additional 3 hours. After that, the cell culture solution was completely discarded, and 200 ul of dimethyl sulfoxide (DMSO; Sigma D2650, USA) was treated and stirred for 200 ul per well, and 100 ul of each was taken as 96 wells to take Enzyme-Linked Immunosorbent Assay. Absorbance at 570 nm was measured by (ELISA). Serum-free medium was used as a control, and 1% of serum was added as a control group. After performing the experiment three times, the average value was calculated and summarized in Table 7.

Type of sample Cell proliferation rate after 2 days (%) Control group (serum-free medium) 100 Comparative group (serum 1%) 176 Example 1 (1%) 128 Example 2 (1%) 131 Example 3 (1%) 126 Example 4 (1%) 139 Example 5 (1%) 137 Example 6 (1%) 124 Example 7 (1%) 129

As shown in Table 7, it was found that all of Examples 1 to 7 have an effect of promoting cell proliferation and can be used for skin regeneration.

From the above description, those skilled in the art to which the present invention pertains will appreciate that the present invention may be implemented in other specific forms without changing its technical spirit or essential features. In this regard, the embodiments described above should be understood as illustrative in all respects and not restrictive. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the following claims rather than the detailed description and equivalent concepts thereof.

Claims (14)

A cosmetic composition comprising as an active ingredient, edible, bovine, rhubarb, osuyu, opilcho, hyangchunja or Kawa extract.
The cosmetic composition according to claim 1, wherein the composition is for antioxidant.
The cosmetic composition according to claim 1, wherein the composition is for moisturizing the skin.
According to claim 1, The composition is for skin whitening, cosmetic composition.
The cosmetic composition according to claim 1, wherein the composition is for improving skin trouble.
According to claim 1, The composition is for improving wrinkles, cosmetic composition.
The cosmetic composition according to claim 1, wherein the composition is for enhancing skin elasticity.
The cosmetic composition according to claim 1, wherein the composition is for skin regeneration.
The cosmetic composition according to any one of claims 1 to 8, wherein the composition has anti-glycosylation, melanin reduction, collagen synthesis promotion, elastase activity inhibition, or cell proliferation effect.
The composition according to any one of claims 1 to 8, wherein the composition is a solution, external ointment, cream, foam, nutrient makeup, softening makeup, pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent , Sunscreen cream, sun oil, suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleaning, oil, powder foundation, emulsion foundation, wax foundation, patch and spray The cosmetic composition having a formulation.
Food composition comprising as an active ingredient, edible, bovine, rhubarb, osuyu, opilcho, hyangchunja or Kawa extract.
The composition of claim 11, wherein the composition is for antioxidant; For skin moisturizing; For skin whitening; For improving skin problems; Wrinkle improvement; For skin elasticity enhancement; Or for skin regeneration, food composition.
A quasi-drug composition comprising as an active ingredient, edible, bovine, rhubarb, osuyu, opilcho, hyangchunja or kawa extract.
14. The method of claim 13, wherein the composition is for antioxidant; For skin moisturizing; For skin whitening; For improving skin problems; Wrinkle improvement; For skin elasticity enhancement; Or for skin regeneration, food composition.