KR100328975B1 - Whitening composition including the myrrh extract - Google Patents

Abstract

The present invention relates to a composition for excellent skin whitening effect, and more particularly to a whitening composition comprising a myrrh extract as an active ingredient.

The composition for whitening containing a myrrh extract according to the present invention is very safe when used in humans and has an excellent effect of reducing pigmentation. Meanwhile, a mixed extract of the myrrh extract according to the present invention is mixed with one or more extracts selected from the group consisting of pagoji extract, sesame oil extract, cheonnyeonja extract, motherwort seed extract, daily Angelica seed extract, motherwort extract, daily Angelica extract and licorice extract If contained, synergistic effect is shown in terms of whitening effect. In addition, myrrh and mixed extract according to the present invention has an effect of reducing pigmentation at a concentration that does not affect the stability of the product can be used as an effective concentration in whitening cosmetics.

Description

Whitening composition including myrrh extract

The present invention relates to a composition having excellent skin whitening effect, and more particularly to a whitening composition comprising a myrrh extract.

Many factors are involved in determining the skin color of humans, including the activity of melanocytes that make melanin, the distribution of blood vessels, the thickness of skin and the presence or absence of pigments in the body, such as carotenoids and bilirubin. It is important. In particular, the most important factor is a black pigment called melanin produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. Genetic factors, physiological factors related to hormone secretion, stress, and the like, and environmental factors such as ultraviolet ray irradiation have an influence on the formation of this melanin pigment.

In particular, ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, or the like to treat or alleviate excessive melanin pigmentation caused by abnormal skin pigmentation symptoms such as blemishes, freckles, pigmentation, and UV exposure. These derivatives and substances with tyrosinase inhibitory activity have been used in cosmetics and pharmaceuticals, but their use is limited due to insufficient whitening effects, safety problems for skin, formulation and stability problems when formulated in cosmetics, etc. It is becoming.

Meanwhile, in addition to these existing whitening efficacy substances, research is being conducted to find whitening active ingredients in natural products. Among them, Sangbaekpi (Japanese Patent Publication No. 55-44375, So64-26507, So64-83009, Pyeong1- 1) 25687, Hei 5-139950, Korean Laid-Open Patent Nos. 92-002109, 97-021273), Licorice (Japanese Laid-Open Patent Publication No. 60-214721, Small 63-23809, Small 64-63506, Flat 1-49706, Korean Publication Patent Nos. 92-002109, 97-025601) and many plant extracts have been found to act on tyrosinase to inhibit melanin production, but they also have cosmetics in terms of safety, stability, and discoloration. There are many problems in using the drug at an effective concentration or higher, and it does not produce a satisfactory effect.

On the other hand, conventionally, a substance that inhibits melanogenesis was searched by searching for a whitening substance as an inhibitory activity against tyrosinase derived from mushrooms. However, since tyrosinase derived from mushrooms is significantly different in structure from tyrosinase, which is responsible for the synthesis of human melanin, the search by mushroom-derived tyrosinase zero shows satisfactory effects in humans. Hard to find whitening material

Therefore, the inventors of the present invention have tried to find a plant extract that can exert a whitening effect even at low concentrations in view of the fact that a natural extract cannot be used at a high concentration in terms of stability of the product, and also, in order to search for a melanogenesis inhibitor that is effective for humans, In order to overcome the problems of the conventional method using a mushroom-derived tyrosinase, a method for measuring the inhibition of melanin biosynthesis at the cellular level, which is an improved method, called Mel-Ab assay, was introduced.

The present invention relates to a composition having excellent skin whitening effect, and more particularly to a composition for whitening, characterized in that the myrrh extract as an active ingredient.

The composition for whitening myrrh extract-containing whitening according to the present invention, at least one extract selected from the group consisting of pagoji extract, sesame oil extract, cheonnyeonja extract, motherwort seed extract, daily Angelica seed extract, motherwort extract, daily Angelica extract and licorice extract It is characterized in that the whitening effect is increased by first adding. In order to search for a melanogenesis inhibitor that is effective for humans, a mouse-derived melanosite (Mel-Ab), which is similar to human melanocytes, can be used. The whitening effect of various plant extracts was searched.

As a result, the myrrh extract not only acts as a very good melanin inhibitor at low concentrations, but also finds a clear whitening effect as a result of direct application to human skin, thus completing the present invention [see Experiments 1 and 2]. ].

Therefore, the present invention is to provide a composition comprising a myrrh extract as an active ingredient, in order to exhibit a skin lightening effect. In addition, the present invention, in order to show the skin whitening effect, at least one extract selected from the group consisting of Pagoji extract, sesame oil extract, cheonnyeonja extract, motherwort seed extract, daily Angelica seed extract, motherwort extract, daily Angelica extract and licorice extract in myrrh extract It is to further provide a composition characterized in that the addition to increase the whitening effect.

Hereinafter, the composition for whitening centering on the technical content of this invention which can achieve the said objective is demonstrated.

Myrrh, the herbal material used in the present invention, is a fragrant medicinal tree that is hardened by spontaneous wounds or spontaneously flows down the trunk of the myrrh tree ( Commiphora myrrha , C. molmol). It is also used as astringent, puncture, and dry matter.

Pagoji is a seed of Psoralea corylifolia , which is said to help renal and reproductive functions in Chinese medicine, to treat Baekjeok for external use, and to strengthen bones.

Fructus is an immature fruit of the medicinal herb , Evodia officinalis ( E. rutaecarpa ), which is used to treat vomiting, headaches, stomatitis, and toothache by warming the stomach, relieving pain, and regulating the circulation of the stomach. do.

The medicinal herb is a fruit of Melia azedarach or M. toosendan, and has been used as a medicine for long-term use as a medicine and pain medicine in traditional medicine.

Motherwort seed is a seed of the plant Lamiaceae ( Leonurus sibiricus ). On the other hand, motherwort is used to help blood circulation in herbal medicine, postnatal prenatal protection, diuresis, indigestion, etc. and for wound healing, but the seed was not used for medicinal purposes. In the case of seeds, the essential oil component is contained in a large amount.

One-danged seed is the seed of the Angelica acutiloba, a flowering plant. On the other hand, Dangdang is used as a blood, tonic, analgesic, sedative in herbal medicine, but its seed was not used for medicinal purposes. In the case of seeds, the essential oil component is contained in a large amount.

Such natural plants were not previously known to have a whitening effect, and furthermore, there was no example where the extract of the natural plant was applied for the whitening effect. In particular, although patents relating to whitening efficacy have been reported for motherwort and single-ear, no application has yet been applied for the whitening effect on the seed, seed. As a ratio, through the present invention, it has been found that the extracts of such natural plants have a whitening effect even at low concentrations.

As a natural plant used in the present invention, the herbal medicine does not limit the term herbal medicine itself, and the "extract" of each natural plant is obtained by partially or completely condensing the leaching liquid obtained by leaching or transferring from the herbal medicine, or again. Concentrates, or acupuncture, premises, regular, flow extracts (hereinafter referred to as "extract extract") prepared by drying the concentrates again, and the chemicals themselves exhibiting the main effect contained in the herbal medicine.

As a natural plant extraction method of the present invention, it is possible to use a known method. For example, the natural plant according to the present invention may be dried and chopped by any method such as natural drying or forced drying, followed by polar solvents such as water, ethanol, butanol and acetone; Nonpolar solvents such as ether, hexane, benzene, chloroform and ethyl acetate; A mixed solvent of the polar solvent and the nonpolar solvent; Using a solvent such as alkaline water, or vegetable oils such as soybean oil and sesame oil, the leaching treatment is carried out by any method such as cold sedimentation, percolation, and warm sedimentation to obtain a leaching substance containing the active ingredient. The leaching treatment is about 12 to 96 hours for cold acupuncture and percolation, and depending on the type and temperature of the solvent used for warm needles, but suitably about 0.5 to 24 hours at a temperature close to the reflux temperature of the solvent. Good to do. In particular, the extracts of the present invention is preferably used tincture, liquid extract or extract of the leaching in the hydrous alcohol.

On the other hand, the amount of myrrh or mixed extract according to the present invention is preferably 0.01 to 10.0% by weight when used in the cosmetic composition. It is because the effect cannot be expected in 0.01 weight% or less, and when it is 10.0 weight% or more, it is difficult to produce safety or formulation form.

In addition, the composition for whitening according to the present invention may also contain other whitening ingredients, etc., which may give a synergistic effect to the main effect, in addition to the above-mentioned extracts within the range of not impairing the main effect of the present invention. It's okay. Examples of such components include kojic acid, arbutin, ascorbic acid derivatives, and the like.

Natural plant extract according to the present invention is suitable to apply the ethanol dilution of the leaching solution or leaching solution as a whitening cosmetic formulation as it is, but conventional herbal preparations, acupuncture, premise, tin, extract, fluid extract, syrup, ointment, It can also be used as an internal or external preparation such as a cream preparation. Such formulations may contain skin absorption promoting substances to increase the whitening effect. In addition, the dosage of these preparations varies depending on the subject's age, symptoms, and method of administration, but in the case of external preparations, 1.0 to 3.0 ml per day is preferably applied 1 to 5 times a day and continued for 1 month or more.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated more concretely based on the Example of this invention. However, it should be noted that the present invention is not limited to these examples.

Preparation of Natural Plant Extracts

1. Preparation of Myrrh Extract 50 g of myrrh was cut into 100 ml of 95% hydrous alcohol based on dry weight. Cooling capacitor was installed to prevent evaporation of the active ingredient in a state of heating for 10 hours to 50 ~ 100 ℃ to extract the active ingredient, filtered and concentrated under reduced pressure completely to obtain 4.5g myrrh extract. The prepared myrrh extract has the form of a viscous red liquid paste and has a unique odor.

2. Preparation of Pagoji Extract

50 g of shredded paper is chopped and 100 ml of 95% hydrous alcohol is added to the dry weight. A cooling capacitor was installed to prevent evaporation of the active ingredient, and then heated to 50-100 ° C. for 10 hours to extract the active ingredient, filtered, concentrated under reduced pressure, and dried to obtain 3.7 g of pagoji extract. Pagoji extract prepared in the form of a viscous brown liquid paste, has a unique odor.

3. Preparation of Sewage Extract

Cut 50 g of sewage oil and add 100 ml of 95% hydrous alcohol to dry weight. A cooling capacitor was installed to prevent evaporation of the active ingredient, and then heated to 50-100 ° C. for 10 hours to extract the active ingredient, filtered, and concentrated under reduced pressure to obtain 6.3 g of effluent oil. The prepared sewage oil extract has the form of a viscous red liquid paste and has a unique odor.

4. Preparation of Stellar Extract

Cut 50 g of the puncturer and add 100 ml of 95% hydrous alcohol to dry weight. A cooling capacitor was installed to prevent evaporation of the active ingredient, and heated to 50 to 100 ° C. for 10 hours to extract the active ingredient, filter it, and concentrated under reduced pressure to dry it to obtain 2.1 g of Stylus extract. The prepared lotus leaf extract has the form of a viscous brown liquid paste and has a unique odor.

5. Preparation of Motherwort Seed Extract

10 g of motherwort seed is added to 100 ml of 95% hydrous alcohol with respect to dry weight. A cooling capacitor was installed to prevent evaporation of the active ingredient, and then heated to 50 to 100 ° C. for 10 hours to extract the active ingredient, filtered, concentrated under reduced pressure, and dried to obtain 0.25 g of motherwort seed extract. The motherwort seed extract prepared is in the form of a viscous dark brown liquid paste and has a unique odor.

6. Preparation of Iltangguise Seed Extract

100 g of 95% hydrous alcohol is added to the dry weight of 10 g of perilla seed. A cooling capacitor was installed to prevent evaporation of the active ingredient, and then heated to 50 to 100 ° C. for 10 hours to extract and filter the active ingredient, concentrated under reduced pressure, and dried to obtain 0.3 g of myrrh. The manufactured Dang-Dang-Gui Seed extract has the form of a viscous brown liquid paste and has a unique odor. Preparation of Motherwort, Daily Angelica, Licorice Extract The extracts are prepared according to what is known in the art.

<Experiment 1; Whitening Screening of Natural Plant Extracts>

Rat pigment cells derived from C57BL / 6 mice were placed in Dulbeccos modified Eagles media (DMEM) with 10% PBS (fetal bovine serum), 10 nM 12-O-tetradecanoylphorball-13-. Incubated at 37 ° C. and 5% CO ₂ in medium containing acetate (12-O-tetradecanoylphorbol-13-acetate) and 1 nM cholera toxin. After culturing the cells at a concentration of 10 ⁵ cells / well in 24 well plates and confirming cell adhesion, the extracted test substances were mixed in the cell culture medium as shown in Table 1 to a final concentration of 0.001% to 0.004%. Incubated for 3 days. The culture solution was removed, washed with PBS, and the cells were dissolved with 1N NaOH and absorbance was measured at 400 nm. Using the cells without the test substance as a control, the whitening effect was measured by measuring the degree of inhibition of melanin production of the test substance compared to the melanin content in the control.

In addition, as a comparative example, the whitening effect of the lettuce extract, licorice extract, motherwort extract, perilla extract and kojic acid extracted by the same method was measured using the C57BL / 6 mouse pigment cells in the same manner.

The melanin production inhibitory effect of the plant extract obtained from it is shown in Table 1 below.

As shown in Table 1, the myrrh extract according to the present invention showed a very high melanin production inhibitory effect at a low concentration of 0.001%. In addition, these extracts were superior in effect at low concentrations compared to kojic acid, which is already known to have excellent whitening effect. In particular, the motherwort seed extract and Ildang eared seed extract were relatively superior in whitening effect as compared to motherwort extract and Ildang ear extract known to have a whitening effect. In addition to the myrrh extract according to the present invention, the whitening effect was increased when selectively added other natural plant extracts, such as pagoji extract and well-known extracts, such as licorice extract.

Example 1 Preparation of Nutritious Cosmetic Water Containing Myrrh Extract

Prescription examples of nutrient cosmetics containing myrrh extract are shown in Table 2.

Example 2 Preparation of Nutritious Cosmetic Water Containing Pagoji Extract

Prepared by the same prescription method as in Example 1, by replacing the myrrh extract with the prepared pagoji extract was prepared nutrient cosmetics.

Example 3 Preparation of Nutritious Cosmetic Water Containing Sewage Oil Extract

Prepared by the same prescription method as in Example 1, by replacing the pagoji extract with the prepared sewage extract was prepared nutrient cosmetic water.

Example 4 Preparation of Nutritious Cosmetic Water Containing Cheonnyeonja Extract

Prepared by the same prescription method as in Example 1, by replacing the pagoji extract with the prepared cheonnyeonja extract was prepared nutrient cosmetics.

Example 5 Preparation of Nutritious Cosmetic Water Containing Motherwort Seed Extract

Prepared by the same prescription method as in Example 1, by replacing the pagoji extract with the prepared motherwort seed extract was prepared nutrient cosmetics.

Example 6 Preparation of Nutritious Cosmetic Water Containing Extracts

Prepared by the same prescription method as in Example 1, by replacing the Pagoji extract with the prepared Dangdangwi seed extract was prepared nutrient cosmetics.

Example 7 Preparation of Nutrients Containing Myrrh Extract and Pagoji Extract

Prepared by the same prescription method as in Example 1, instead of the myrrh extract was prepared nutrient cosmetics containing a mixed extract of 0.5 each of the myrrh extract and the pagoji extract was mixed.

Example 8 Preparation of Nutritious Cosmetic Water Containing Myrrh Extract, Pagoji Extract and Sewage Extract

Prepared by the same prescription method as in Example 1, instead of the myrrh extract was prepared nutrient cosmetics containing a mixed extract of 0.5 each of the myrrh extract, the pagoji extract and the sewage oil extract.

Example 9 Preparation of Nutrients Extract Containing Myrrh Extract, Pagoji Extract, Lilium Extract, and Licorice Extract

Prepared by the same prescription method as in Example 1, instead of the myrrh extract was prepared nutrient cosmetics containing a mixed extract of 0.5 each of the myrrh extract, the pagoji extract, the cheongija extract and a known licorice extract.

Example 10 Preparation of Nutritious Cosmetic Water Containing Motherwort Seed Extract and Motherwort Extract

Prepared by the same prescription method as in Example 1, the myrrh extract was mixed with each of the prepared motherwort seed extract and known motherwort extract, respectively 0.5, and replaced to prepare nutrient cosmetics.

Example 11 Preparation of Nutritious Cosmetic Water Containing Extracts of Il-Dang-Gui

Prepared by the same prescription method as in Example 1, by mixing and replacing each of the myrrh extract with 0.5 mg each of the prepared perilla extract and known periwinkle extract to prepare nutrient cosmetics.

Comparative Example 1: Preparation of Nutritious Cosmetic Water Containing Kojisan

It was prepared by the same prescription method as in Example 1, but instead of kojic acid instead of the myrrh extract was prepared for nutrient cosmetics.

<Experiment 2; Whitening Effect Test on Human Body of Prescription Formulation Containing Natural Extracts ＞

Using 20 healthy men and women as test subjects, an opaque tape with 6 holes of 1.5 cm in diameter was attached to the lower arm of each arm and irradiated with UVB (1.5-2 times the minimum erythema) of each subject. After induction of blackening, nutritional longevity containing the test substances (Examples 1 to 11 and Comparative Example 1) was corrected twice a day for 8 weeks (morning and evening) and 5 days a week. Two months later, the contrast of the skin was measured using a color difference meter. The control group was prepared in the same manner as in Example 1, but was tested by applying nutrient cosmetics that the natural plant extract is not added to the components.

The effect was determined by measuring the degree of black and white of the skin using a color difference meter (Minolta CR2002). L ^* a ^* b ^* color system is used to display the color. In this experiment, L ^* value (brightness) is mainly used as an index. The L ^* value was calibrated with a predetermined white board, and the measurement was performed by measuring the pigmentation portion evenly by repeating the measurement five times or more at one place. The difference in skin color (ΔL ^* ) at the time of starting application of the nutrient cosmetics containing the test substance and the completion of the application of the nutrient cosmetics after 8 weeks was determined and the effect was determined using this value. As a result, the whitening effect at the human level of the natural plant extract is shown in Table 3.

ΔL ^* = L ^* after X days after application - L ^* values at the time of start of the coating

As shown in Table 3, when the nutrient cosmetics containing the myrrh extract and the like according to the present invention was used, it was confirmed that the reduction of skin pigmentation. In addition, in the case of the formulation prepared by mixing the myrrh extract with other natural extracts such as pagoji extract and known natural extracts such as licorice extract, the whitening effect was shown to be increased. During this experiment, the subject showed no irritation by the use of nutrient-containing water containing plant extracts.

<Experiment 3: safety test, human patch test>

In order to determine the degree of skin irritation with respect to the composition according to the present invention, a skin patch test on the whitening cosmetics according to Examples 1 to 11 was performed on 15 subjects. 20 µl of each of the nutrients of Examples 1 to 11 was added to a fin chamber and patched to the upper arm of the human body for 24 hours, the skin reaction was examined within 1 hour after the patch was removed, and the next day (after 48 hours) was examined again. . The skin response was judged by the inspection criteria of Table 4, and the average response rate was calculated according to the following formula.

Average response rate (%) = [ Σ (X score _{after 24 hours} of respondents) + Σ (X score _{after 48 hours of} respondents)] × 100 / (Total number of tests × Maximum score × all subjects)

For example, the average reaction rate of Example 1 was calculated as follows.

((14 x 0 points + 1 x 1 point + 0 x 2 points + 0 x 3 points + 0 x 4 points) + (15 x 0 points + 0 x 1 point + 0 x 2) Dot + 0 points x 3 points + 0 points x 4 points) x 100 / (2 x 4 points x 15 persons) = 0.83%

In addition, in Example 2 to Example 11 was also calculated the average reaction rate in the same manner as described above. As a result, the results of the human patch test of the whitening cosmetic composition are shown in Table 5 below.

Less than 1: non-irritating, less than 1 to 3: hard stimulation, less than 3 to 5: medium stimulation,

5 or higher: strong stimulus

As can be seen from Table 5, the whitening cosmetic composition according to the present invention can be said to be a safe substance to the human body because it belongs to the non-irritating range.

<Experiment 5: Stability Test>

In order to determine the degree of stability for the composition according to the present invention, a stability test for the whitening cosmetics according to Examples 1 to 11 was carried out.

Nutrients of Examples 1 to 11 were allowed to stand for 3 months in a room temperature, 4 ℃ refrigerator, 37 ℃ thermostat and 45 ℃ thermostat, after 3 months, the change of each formulation was observed. According to the degree of separation of the formulation, '0' (stable), '+/-' (slightly unstable), and '+' (unstable).

As shown in Table 6, the whitening cosmetic compositions according to the present invention were stable at room temperature (25 ° C), cold (4 ° C), 37 ° C and 45 ° C.

As can be seen from the examples and experiments according to the present invention, the composition for whitening containing a myrrh extract or a mixed extract according to the present invention is very safe when used in humans and is excellent in reducing pigmentation. On the other hand, synergistic effect in terms of the whitening effect is shown by containing a mixed extract prepared by mixing the myrrh extract and other natural extracts (such as pagoji extract, etc.) and known whitening substances (such as licorice extract). In addition, natural plant extracts according to the present invention can be used in effective whitening cosmetics because it has an effect of reducing pigmentation at a concentration that does not affect the stability of the product.

Claims (3)

A whitening composition comprising a myrrh extract as an active ingredient. According to claim 1, Pagoji extract, sesame oil extract, cheonnyeonja extract, motherwort seed extract, daily Angelica seed extract, motherwort extract, daily Angelica extract and licorice extract by adding one or more extracts selected from the group to increase the whitening effect Whitening composition characterized by. The composition for whitening according to claim 1 or 2, wherein the composition is a cosmetic composition, and the extract contains 0.01 to 10.0 wt%.