KR20090017282A - Cosmetic composition containing a medicinal liquor extracts of traditional oriental herb complex with the antioxidant effect - Google Patents

Abstract

A cosmetic composition containing the extract of Oriental herb medicinal liquor is provided to protect the skin from the reactive oxygen and free radicals which are known as principal reason of skin aging, and strengthen the physiological activity of the skin lowered by age, stress, ultraviolet, environment pollution and dryness. The antioxidant cosmetic composition for improving skin wrinkles contains the extract of Oriental herb medicinal liquor including Angelica gigas Nakai, Paeoniae lactiflora var. trichocarpa BUNGE., Moutau Cortex, Hoelen Rubra, Longanae Arillus, Carthami Flos, Cyperi Rhizoma, Gardeniae Fructus, Menthae Herba, Bupleuri Radix, Chrysanthemum morifolium, Zizyphi Fructus and honey.

Description

Cosmetic composition containing a herbal medicine extract with an antioxidant effect {Cosmetic composition containing a medicinal liquor extracts of traditional oriental herb complex with the antioxidant effect}

The present invention is a cosmetic composition containing an herbal composite material having an antioxidant activity, that is, extracts of Angelica, Peony, Peony, Red Bok-Ryong, Yong-An, Safflower, Hyangbuja, Gardenia, Peppermint Leaf, Shiho, Chrysanthemum, Jujube and Honey It relates to a composition.

The primary function of the skin is to protect the human body from external stimuli such as temperature, humidity and ultraviolet light. However, when external stimuli such as strong ultraviolet rays, stress, and nutritional deficiency are received from the outside, the skin may cause aging of the skin such as wrinkle formation, loss of elasticity, and keratinization. Skin aging is largely divided into natural aging (or endogenous aging) and external aging (Doris EB, Cosmet . Toiletries , 1996, 111 , 31), and because natural aging (endogenous aging) is influenced by genetic factors, On the other hand, external aging is relatively easy to artificially adjust because of environmental factors. Representative external aging factors include ultraviolet rays, and the most prominent external aging phenomenon is wrinkle formation (Daniell HW, Ann . Intern . Med . , 1971, 75 (6) , 873; Grove GL et al . , J. Am . Acad .. Dermatol, 1989, 21 ( 3), 631;.. etc. Griffiths CE, Arch Dermatol, 1992, 128, 347).

One of the photoaging mechanisms caused by ultraviolet light is via free radical pathways (Harman D, J. Gerontol . , 1956, 11 (3) , 298). Free radicals are known to cause breakdown of connective tissue formation such as cutaneous collagen, inhibition of cell membrane function, promotion of DNA mutation, modification of protein function, intercellular energy transfer, modification of molecules related to metabolism (Lavker RM and Kligman AM, J. Invest . Dermatol . , 1988, 90 , 325). The fact that free radicals are involved in aging means that antioxidants or various chemical scavengers that inactivate the free radicals can be used to delay the aging process.

On the other hand, even in normal cells, some free radicals and other free radicals and peroxides are generated during metabolism, but superoxide dismutase (SOD) and catalase are used as defense mechanisms in vivo. Along with antioxidant enzymes, such as peroxidase, antioxidants such as vitamin C, vitamin E, glutathione, ubiquinone, and uric acid exist to protect themselves. However, oxidative stress is caused when abnormality is caused in such a bioprotective device or when the production of free radicals exceeds the capacity of the biological defense system by various physical and chemical factors.

Therefore, there is an urgent need for the development of substances that have strong active oxygen inhibitory and antioxidative effects and are safe for human skin (Park SN, J. Soc . Cos . Sci . Kor. , 1997, 23 (1) , 75). .

As a result, many studies have been carried out to develop natural antioxidants of plant origin, which have high antioxidant effects, are guaranteed to be safe for human skin, and are economical (Larson RA, Phytochemistry , 1988, 27 , 969). . Recently, as research on natural products has been actively conducted, secondary metabolites contained in natural products have become a major concern as physiologically active substances, and in particular, studies on antioxidants have been actively conducted (中 谷 延 二, 日本 食品). Industrial Engineering , 1990, 37 , 569). Bioactive substances are high value-added substances that show remarkable activity even in very small amounts, and many kinds are usefully used, and new substances are being actively researched (條 原 和 毅, 食品 と 開發 , 1992, 27 , 29; Kang SS et al., Natural product science . Seoul Univ. Publishers, Seoul, 1988, 71).

Natural antioxidants known to date include tocopherols, flavonoids, gossypol, sesamol, oryzanol and vitamin C (Huson B et al., Food) Chem . , 1987, 19 , 537; Frankel EN, Food Chem . , 1996, 57 , 51; Giese J, Food Technol . , 1996, 5 , 73; Pszcczola DE, Food Tech . , 2001, 55 , 51). Among them, tocopherol and L-ascorbic acid are preferred as natural antioxidants. Among them, tocopherol has high safety but low ability to inhibit oxidation reaction (Halliwell B et al., FASEB J. , 1988, 2 , 2867) The disadvantage is that the price is expensive.

Angelica (當歸) is Manchuria, grows in soils with Japan and South Korea humid mountain valley, central, northern, and has been cultivated as a medicinal plant, Angelica gigas (scientific name: Angelica gigas Nakai), Angelica acutiloba (S. et Z.) Kitagawa) and Chinese Angelica Angelica sinensis (OLIV.) DIELS) are known. Angelica is herbaceous in nature, warm and nonpoisonous, tastes sweet and spicy. Blood, colloquia, landscaping, sedation, body weakness, joint pain, headache, dizziness, abdominal pain, menstrual irregularities, constipation, bruises are used. Water extract of Angelica tang is known to lower blood pressure, diuresis, cerebral sedation, training excitement, and sedation. The ethanol extract of Angelica gigas activating the movement of the uterus, the essential oil solution of the root has pharmacological effects such as analgesic and anti-inflammatory action on arthritis. In addition, the Angelica helps protein synthesis and new uterine tissues, promotes the absorption of glucose, and promotes metabolism, and regenerates bone marrow to help hematopoiesis. Angelica gigas contains 2-3% and 1.38% by weight of coumarin in the root and outpost, respectively. The main components of coumarin are decurcin, decursinol, umbelifenone, and nodakenin. In addition, xanthotoxin, isoprimpinelin, ostol, and umbellininine are known. The root contains 0.3-0.6% by weight essential oils. Berries contain coumarin and essential oils such as decursinol. Preferred sites for use according to the invention are roots.

Peony (芍藥, Paeoniae lactiflora var. Trichocarpa BUNGE.) Uses a full ranunculales, Ranunculaceae, a perennial neck Enforcement (paeonia), medicinal roots for food. The mountains are cultivated everywhere in China, Japan, and Korea. The harvest time is August to October, the taste is bitter, the weakness is plain and nontoxic. It is a perennial herb belonging to the peony family, the roots are short and large, with a node, extending horizontally. Stems are upright, branched, about 50cm high, with several primitive leaves on base. Leaves are regenerated, petioles are long, 3 times 2, small leaves are obovate-oval, sharp at the ends, do not go through, white is white, hairs are on the upper part of veins. Flowers come out one by one at the end of stem and are rather large. Flowers bloom white to May-June. The root is used for medicinal purposes. The shape is fusiform and 10-20 cm long. Ingredients known to date include peonyflorin, peonol, peonine, benzoic acid and essential oils. The known active ingredient of the peony has been identified as phoniflorin. Stomach and intestinal smooth muscle and uterine smooth muscle have the effect of contraction suppression, coronary dilatation and vascular disease against atherosclerosis, blood pressure lowering and blood flow. Preferred sites for use according to the invention are roots.

Neck Peel,Moutau Cortex) Is the Peony family perennial peony (Paeonia suffruticosa The root bark of ANDR. This is an essential drug of gynecological drugs, sedative action, fever lowering action, pain relief action, anti-inflammatory action and bloody action and blood pressure lowering action has been reported. Bark skin is known to show anti-inflammatory effect and blood circulation improvement when used in combination with causality. On the other hand, the efficacy of the endothelial skin associated with cosmetics has a boil treatment effect by anti-inflammatory action, tyrosinase activity inhibitory effect, it is known to prevent skin aging by promoting blood circulation and anti-inflammatory action and astringent action. Preferred sites for use according to the invention are roots.

Poria (茯 笭, Poria cocos WOLF) is a fungal nucleus that parasitic around the roots after 5 to 6 years of resection of pine trees, with pale white as Baekbokryeong and red brown as Hoelen. Rubra ), which contains rosacea, is called fuxin, and its main components are carbohydrates, water, crude fiber, minerals and trace proteins. Recently, many studies have been conducted on anticancer effects. It is reported that triterpene phase in Bokryeong has anti-soil, anti-inflammatory effect, etc. The bacterium contains beta-pachyman, which occupies 93% of dry weight, and triter Pachymic acid, tumulosic acid, tribeta-hydroxylanosta-7,9 (3-β-hydroxylanosta-7,9), 24-triene-2- of triterpenoid compounds Acid (24-triene-2-acid), in addition to chitin, sterols, lecithin, adenine, histidine, choline, lipase, proteinase, etc. It contains. Bokryeong acts on the digestive system such as diuretic, antimicrobial and gastric acid, and decreases blood sugar levels (Information, Shin Min-kyo, Doha medicinal metabolism, Yeonglimsa, pp40-43, 1998).

Longane Arillus ) is a dried bark of Euphoria longan (LOUR.) STEUD., Which contains glucose, tartaric acid, vitamins B1, B2, P, and C. Calm, healthy, nourishing, insomnia is treated.

Safflower (紅花, Carthami Flos ) Safflower ( Carthamus) tinctorius L.) is a flower. The taste is sour and the nature is warm. Safflower contains safflower yellow, carthamin and 15α, 20β-dihydroxy- Δ ⁴ -pregnen-3-one. Safflower oil is a glyceride such as palmitic acid, stearic acid, arachidic acid, oleic acid, linoleic acid, and the like. The leaves contain luteolin-7-glucoside (Joo Jung-seop and Shin Mingyo; Ilbo Herbal Medicine, Daelim, Yeonglim, pp. 1034-1035, 1998). Safflower seed has been used for medicinal purposes such as soothing and bleeding in Korea, China and Japan (Han, Dae-Seok; Herbal Medicine, 1995), and is a useful resource plant grown for cooking oil production, especially in the US and India (Andeok, Yuk-Soo; Modern Herbology, 1975). In particular, safflower oil contains a large amount of unsaturated fatty acid and tocopherol, including linolenic acid, which is nutritionally important and widely used as an antioxidant, antithrombotic, and antihypertensive new material (In-Kyu Park, Bong-je Kim, Dong Bo-gam, 1989).

Cyperi Rhizoma is a rhizome of Cyperus perennial herbaceous Cyperus rotundus L., essential oil, alkaloid, flavonoid, sugar and pectin. pectin) and the like. Flow extract of the root stem of Hyangbu is known to improve pain, pain relief, uterine tension, cardiac action, urination and blood circulation. In oriental medicine, Hyangbuja is a pain medication and a blood shading drug, which is widely used for dysmenorrhea and postpartum gynecological diseases. It is also used in antipyretic medications and digestive medicines for colds, head aches and embryos. Related to cosmetics, Hyangbuja shows strong antibacterial effects on skin fungi, smoothes blood circulation, brightens the color, and is known to prevent skin aging due to antioxidant and anti-inflammatory effects.

Gardeniae Fructus) is Gardenia belongs to the Rubiaceae (Rubiaceae) tree (Gardenia jasminoides for. Grandiflora MAKINO), an oval upside down egg, ripened in yellow in September. In addition, there are 6 ridges about 2cm long, calyx remains on top and does not split when mature. Inside is yellow pulp and seeds. In oriental medicine, it is used to treat insomnia and jaundice, and is known to have anti-inflammatory, hemostatic and diuretic effects. In addition, it is used as a colorant for food, and in the old days, it was stored in gardenia soaked in gardenia to prevent the deterioration of military taste.

Mint ( 薄荷 , Menthae Herba ) mint ( Mentha arvensis L. var. piperascens MALINV.) is an outpost or leaf. It contains 70-90% by weight of menthol and 1% by weight of essential oils. Other essential oil components include isomentone, neomenthol, pinene, camphor, L-limonene and the like, and high boiling point components include gamma-hexenolphenyl acetate and ethyl amyl ketone. The essential oils of peppermint vary depending on the variety. Recently, various kinds of peppermint are known as linalool. Menthol, the main ingredient of peppermint oil, is medicinal as a coating agent, analgesic agent, stimulant agent, and nutrient agent, and is used as a fragrance or refreshing agent in toothpaste, candy, jam, cosmetics, tobacco, and the like. Preferred sites for use according to the invention are leaves.

Shiho, Bupleuri Radix ) are roots of the same greenery as Siho. The taste is bitter and the temper is light. Sea roots contain essential oils, bupleurumol, oleic acid, linoleic acid, palmitic acid, stearic acid, lignoceric acid and saponin (saponin) and the like. Saponins include saikosaponin A, C, D, saikogenin F, E, G, and longispinogenin. Shiho has antipyretic, calming, analgesic, anti-inflammatory, and anti-pathogenic effects (Ji, Jung-Seop and Shin, Min-Kyo; Doha Herbal Medicine (Medicinal Herbs), Yeonglimsa, pp412-413, 1998).

Chrysanthemum ( Chrysanthemi Flos ) refers to the flowers of Chrysanthemum morifolium RAMAT., Which is distributed in Korea, Taiwan, China, and Japan, and mainly grows in the mountains. It has short hairs all over the grass, and the stem is 60 s and 90 cm in height and is black and thin. Leaves are dark green, alternate, petioles, egg-shaped, usually divided into feathers and pointed. Cracks are long oval and have serrated teeth. In October, the flowers are dried and drunk. Flowers contain 0.13% by weight of essential oils, including Borneol, Bornyl Acetate Diecampa, Tetracozan, Hexacozan, etc., and acecetin compound, glucoside, adenine, choline, and carbohydrate.

Jujube ( Zizyphi Fructus ) jujube tree ( Zizyphus) jujuba MILL. Var. inermis (BGE.) REHD.), also known as crude (목) or wood wheat (木 蜜). The surface is reddish brown, elliptical, 1.5 ~ 2.5 cm long, and ripe when red. The jujube fruit has 20-49% dry matter, and 14-42% is composed of sugars such as fructose, sucrose, glucose and rhamnose. In addition, organic acids such as malic acid, grape acid, succinic acid and glycolic acid are contained 0.54 to 3.4% and are rich in minerals such as potassium, calcium and magnesium. Fruits are not only reproduced, but also processed in life and used as jujube, jujube tea, jujube vinegar and jujube. In oriental medicine, it is used as a diuretic, tonic, and emollient. In addition, in oriental medicine, the muscles are aching disease, when the muscles are painful, cough, when the whole body hurts are mainly used and is known to be effective in exfoliating skin with anti-allergic effect.

Honey is a translucent, glossy, sticky liquid, white or pale yellow or orange yellow. Depending on the storage method, white granular crystals may form. It has an unusual aroma and tastes sweet. Generally, fructose and glucose are 70% by weight and contain sugar, maltose and organic acids.

Accordingly, the present inventors have made a result of the diligent effort, the herbal complex combination herbal extracts containing Angelica, Peony, Peony, Red Bokryingyeong, Longan meat, safflower, Hyangbuja, Gardenia, Peppermint Leaf, Shiho, Chrysanthemum, Jujube and Honey have active oxygen scavenging activity. By confirming, the present invention was completed.

Therefore, an object of the present invention, the herbal extracts containing Angelica, Peony, Peony, Red Bok, Ryongan, Safflower, Hyangbuja, Gardenia, Peppermint Leaf, Shiho, Chrysanthemum, Jujube and Honey having active oxygen scavenging activity as an active ingredient It is to provide a cosmetic composition comprising a.

In order to achieve the above object, the present invention provides a cosmetic composition containing an herbal complex pharmaceutical extract having an antioxidant activity as an active ingredient.

Herbal composite materials as defined herein are Angelica gigas Nakai), peony (Paeoniae lactiflora var. trichocarpa BUNGE. ), mokdanpi (Moutau Cortex ), Hoelen Rubra ), Longanae Arillus ), safflower ( Carthami Flos ), Cyperi Rhizoma ), Gardeniae Fructus ), mint ( Menthae Herba ), Bupleuri Radix ), Chrysanthemi Flos ), Jujube ( Zizyphi Fructus ) and honey.

Hereinafter, the present invention will be described in detail.

The present invention is a herbal composite material having antioxidant activity, that is, Angelica gigas Nakai, Peony (Paeoniae lactiflora var. Herbal complex herbal extracts including Carthami Flos, Cyperi Rhizoma, Gardeniae Fructus, Peppermint (Menthae Herba), Bupleuri Radix, Chrysanthemi Flos, Jujube (Zizyphi Fructus) and Honey It provides a cosmetic composition comprising as a component.

Herbal complex medicinal extract as defined herein refers to an extract of the active ingredient of the complex herbal medicine prepared by oriental medical prescription by dissolving and leaching with an alcohol solvent.

Extract of the present invention can be obtained by the following manufacturing process.

Except honey, the dried herbs are washed to remove foreign substances and the like present on the surface of the material, and then dried and ground. The ground herbal medicines, together with honey, are placed in the extraction container in an appropriate content ratio. The mixed herbal sample is a mixed solvent having a mixture ratio of C ₁ to C ₄ lower alcohols such as water, ethanol, methanol, and the like, polyhydric alcohols such as glycol, or about 1: 0.1 to 1:10. Aged at 10 ° C. to 100 ° C., preferably at about 20 ° C. to 60 ° C. for about 1 hour to 30 days, preferably 12 hours to 15 days, at this time, extraction method such as reflux cooling extraction, ultrasonic extraction, warming, and cooling After extraction through, through the filtration, concentration under reduced pressure and drying it can be obtained the herbal extract of the complex combination of the present invention in the filtrate state.

As a method of aging the mixture of the herbal composite material and the solvent, there is a method of using a reflux apparatus in a water bath of about 10 ℃ to 100 ℃, but not limited to this method if it can achieve the same effect It can be obtained by selecting a method known in the art.

In addition, the extraction is used by the ultrasonic extraction method, the ultrasonic wave is preferably treated for 1 to 5 times a day, 10 minutes to 15 hours, maintaining a low temperature below 60 ℃ during the aging period.

Ultrasound as defined herein refers to high frequency sounds that are inaudible to the human ear. Extraction method using ultrasonic waves as described above stabilizes the unstable structure of various useful ingredients, and can be applied to various ranges of ultrasonic waves according to the properties of herbal raw materials by adjusting the intensity of energy change, so that effective ingredients of herbal raw materials can be applied more effectively. It has the advantage of being able to extract.

In addition, the low temperature defined herein means 60 degrees C or less. Such a temperature is a temperature range in which changes in the composition during extraction can be minimized.

The content ratio of each component of the herbal composite material may vary depending on the desired effect such as skin improvement, skin protection. In one embodiment according to the present invention, based on 100% by weight of the combined weight of the combined herbal medicine, preferably 3 to 20% by weight, peony 3-20% by weight, 3-20% by weight of bark, 3-20% by weight of red meat, 3-10% by weight of dragon's meat, 3-20% by weight of safflower, 3-20% by weight of Hyangbuja, 2-20% by weight of Gardenia jasminoides, 2-20% by weight of mint leaves, 2-20% by weight of Shiho, 2 to 20% by weight of chrysanthemum, 3 to 20% by weight of jujube and 3 to 50% by weight of honey may be mixed.

As a solvent for the preparation of the extract of the present invention, at least one solvent selected from the group consisting of purified water, C ₁ to C ₄ lower alcohols, polyhydric alcohols or mixtures thereof is preferable, and the polyhydric alcohol is propylene glycol, butylene glycol. Or pentylene glycol, but is not limited thereto. As a more preferable solvent, the ethanol aqueous solution containing 10-90 weight% ethanol with respect to a solvent total weight is mentioned. At this time, when the aqueous solution of ethanol is less than 10% by weight of ethanol, it is difficult to obtain an extract containing the desired substance from the herbal material, and when the aqueous solution of more than 90% by weight of ethanol, an extract containing unwanted components is obtained. There is a problem.

Antioxidative Effect of the Herbal Combination Herbal Medicine Extract of the Present Invention by Measuring the DNA Protective Activity in the Presence of Active Oxygen Species (Hydrical Radicals and Superoxide Anion Radicals) Scavenging Activity and Active Oxygen Species It was confirmed. In addition, collagenase inhibitory activity was measured to confirm the wrinkle improvement effect.

In addition, the medicinal herbs constituting the herbal herbal medicine extract is a drug that has been used for a long time as a herbal medicine and edible extracts of the present invention extracted from them also have no problems such as toxicity and side effects, safety has been proved even when used for a long time Can be used.

The present invention provides a cosmetic composition containing the herbal composite medicine extract obtained by the above manufacturing process as an active ingredient. Preferably, the extract of the present invention is added to the cosmetic composition by adding an organic solvent or purified water containing 1 to 5 times the weight of ethanol, butylene glycol.

The cosmetic composition of the present invention is characterized in that it contains 0.1 to 10% by weight, preferably 5% by weight, based on the total weight of the cosmetic composition. At this time, when less than 0.1% by weight, the skin protection effect against ultraviolet rays is insufficient, and when it contains more than 10% by weight, strong odor and color according to the inherent flavor of the herbal medicine in the final product is strongly indicated for product application There is difficulty.

Cosmetic compositions for preventing aging of the skin according to the present invention may have a conventional formulation, for example water-soluble liquids, lotions, creams, gels, packs, essences, oil in water (O / W), water in water (W / It may be formulated in powder of O) type or water in silicon (W / Si) type. The composition of each of these formulations may contain a variety of bases and additives necessary for the formulation of the formulation and are suitable, and the types and amounts of these components can be readily selected by those skilled in the art.

In addition, the cosmetic composition containing an extract having a skin protective action by the antioxidant effect according to the present invention can be used according to a conventional method of use, the number of times of use may vary depending on the user's skin condition or taste.

By using the cosmetic composition to which the herbal complex medicine extract of the present invention is added, it provides an antioxidant effect and wrinkle improvement effect to protect the skin from free radicals and free radicals known to be the main cause of skin aging in recent years. It can contribute to skin aging prevention, skin protection, and skin improvement by enhancing the physiological activity of skin that has been degraded due to environmental pollution and drying.

Hereinafter, although an Example, a comparative example, a formulation example, and a test example demonstrate this invention more concretely, these are for illustrating this invention, and the scope of the present invention is not limited by these.

Reference Example . Material preparation

Angelica ( Angelica) , a constituent of the complex herbal medicine used in the present invention gigas Nakai), peony (Paeoniae lactiflora var. trichocarpa BUNGE. ), mokdanpi (Moutau Cortex ), Hoelen Rubra ), Longanae Arillus ), safflower ( Carthami Flos ), Cyperi Rhizoma ), Gardeniae Fructus ), mint ( Menthae Herba ), Bupleuri Radix ), Chrysanthemi Flos ) and Jujube ( Zizyphi Fructus ) was purchased from Gyeongdong Market, Seoul, washed with water, and then used in the shade according to the contents recorded in the agreement. In addition, honey was purchased from Gyeongdong Market in Seoul and used after checking for foreign substances.

Example  1. Preparation of Herbal Complex Medicine Ultrasonic Extract

As shown in Table 1 below, on the basis of 100% by weight, the total weight of the dry weight of the composite herbal medicine prepared in the reference example, Angelica, Peony, Peony, Red Bokyong, Yongan, Safflower, Hyangbuja, Gardenia, Peppermint Leaf, Siho, Chrysanthemum , Jujube and honey were prepared in 8.3: 4.4: 3.9: 4.4: 8.3: 3.9: 5.6: 2.8: 2.8: 2.8: 2.8: 5.6: 44.4 wt%.

turn Kinds Dry weight before extraction (g) One Donkey 15 2 Peony 8 3 Neck skin 7 4 Red coat 8 5 Dragon meat 15 6 Hyangbuja 7 7 Safflower 10 8 Gardenia 5 9 Peppermint leaves 5 10 Shiho 5 11 Chrysanthemum 5 12 Jujube 10 13 honey 80

The herbal medicines except honey were first washed with water and then crushed finely. The pulverized product was immersed in a 15% aqueous ethanol solution at 30-35 ° C. for 10 days, and in order to maximize extraction of the active ingredient of the herbal composite material, ultrasonic treatment was applied twice a day for 30 minutes during the immersion period. At this time, the immersion was extracted by sonication while maintaining 60 ° C. or less. Ultrasonic power and extraction time per unit volume of extraction solvent were used as process and system variables. The ultrasonic generator used a high power ultrasonic generator operating at 20 kHz, and the high power ultrasonic generator used 400, 1200, 2400, 4000 W / An output of L was applied.

After extracting for 10 days, the mixture was filtered and concentrated under reduced pressure to obtain 2 g of the herbal combination medicine ultrasonic extract.

Comparative example  1: compound herbal medicine Hydrothermal  Preparation of Extract

The compound herbal prepared in the same manner as in Example 1 was used.

The herbal medicines except honey were first washed with water and then crushed finely. The pulverized product was immersed in a 15% aqueous ethanol solution at 30-35 ° C. for 10 days, extracted, filtered and concentrated under reduced pressure to obtain 2 g of the compound herbal hydrothermal extract.

Comparative example  2 to 14. Preparation of Ultrasonic Extract of Each Herb

The ultrasonic extract of each herbal medicine which comprises the herbal composite material of Example 1 was prepared. The extraction method used the same method as Example 1. 100g of each of Angelica, Peony, Peony, Red Bok-Ryung, Yong-An, Safflower, Hyangbuja, Gardenia, Peppermint Leaf, Shiho, Chrysanthemum and Jujube were prepared by crushing finely and 100g of honey. Each herbal medicine prepared as described above was immersed in 15% aqueous ethanol solution at 30-35 ° C. for 10 days, extracted, filtered and concentrated under reduced pressure to obtain 2 g of ultrasonic extract of each herbal medicine.

Formulation example  One. Example  Containing extract of 1 skin  Lotion

A skin lotion was prepared at the composition ratio as shown in Table 2 below. The alcohol was prepared by dissolving methyl parahydroxybenzoic acid and polyoxyethylene hydrogenated castor oil in ethanol in this order. The purified water portion was prepared by dissolving glycerin, 1,3-butylene glycol, hyaluronic acid, allantoin, citric acid and sodium citrate in the order of sterilized purified water. The skin portion was prepared by mixing the alcohol portion and purified water portion with the composite herbal ultrasonic extract prepared in Example 1.

Classification Raw material name Weight (% by weight) Alcohol part ethanol 7 Methylparahydroxybenzoic acid 0.1 Polyoxyethylene Hydrogenated Castor Oil 0.4 Purified water Purified water 89.51 glycerin 0.1 1,3-butylene glycol 0.3 Hyaluronic acid 0.5 Allantoin 0.05 Citric acid 0.01 Citric acid 0.03 Herbal part Complex herbal ultrasound extract of Example 1 2.0 Sum 100

Formulation example  2. Example  Containing extract of 1 milk  Lotion

To a composition ratio as shown in Table 2 below, the milk lotion was prepared using the same preparation method as in Formulation Example 1.

Classification Raw material name Weight (% by weight) Alcohol part Stearic acid 2.1 Cetanol 1.3 Liquid paraffin 7.0 Squalene 0.5 Isopropyl myristate 1.0 Glyceryl Monostearate 0.5 Arasel-60 0.85 Polyoxyethylene Hydrogenated Castor Oil 0.1 Isostearyl-isostearate 0.5 Propylparaben 0.1 Alanine 200F / 100CS 0.1 Purified water Purified water 73.15 Triethanolamine 0.7 Propylene glycol 7.0 glycerin 3.0 Methylparahydroxybenzoic acid 0.1 Herbal herbal medicine part Complex herbal ultrasound extract of Example 1 2.0 Sum 100

Test Example  One. Formulation example  1, check the skin irritation and protective effect

The skin lotion of Formulation Example 1 was applied to the forearm of 30 healthy males and females (15 males and 15 females) by a predetermined amount (10 m / 5 cm 2), and the rest was shielded with aluminum foil. After 30 minutes, long-wavelength ultraviolet rays were irradiated with an ultraviolet irradiator (ORIEL solar simulator 1000W, Newport corp., USA) at a dose of 10.2 J / cm 2 at a distance of 10 cm from the arm and the skin condition (erythema, edema, papules, etc.) after 24 hours. Was observed. As a result, skin side effects such as erythema did not occur on the skin, and thus the skin lotion did not cause phototoxicity, and it was confirmed that the skin protection effect.

Test Example  2. Formulation example  2, check the skin irritation and protective effect

30 minutes after applying a predetermined amount (10m / 5cm2) of milk lotion of Formulation Example 2 to the forearm of 30 healthy men and women (15 males, 15 females), and shielding the other portions with aluminum foil, Long-wavelength ultraviolet rays were irradiated at a dose of 10.2 J / cm 2 using an ultraviolet irradiator and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, the skin lotion did not cause side effects such as erythema, and thus it was confirmed that the milk lotion did not cause phototoxicity and also had a protective effect on the skin.

Test Example  3. Formulation example  Skin safety confirmation test of 1 and 2

In order to confirm the skin safety of Formulation Examples 1 and 2, a human patch test was performed.

Wipe the forearm of 30 healthy males and females (15 males and 15 females) with 70% ethanol, and place the pin chamber containing 20 μl of Formulation Examples 1 and 2 in the usual cosmetic base on the site. Attached for 24 hours. After 24 hours, the adhesive tape and the pin chamber were removed, and the sample remaining on the skin with a gauze was lightly removed, and then the skin condition was observed, and again after 24 hours. As shown in Table 4, there are no subjects who show a positive response in the human patch test to confirm skin irritation, and the skin lotion and milk lotion of Formulation Examples 1 and 2 containing the complex herbal extract of the present invention It has been confirmed that the skin is not irritating at all.

sample After 24 hours (n = 40) After 48 hours (n = 40) Remarks Skin lotion 0/30 0/30 Number of reaction quantums / total number of targets Milk lotion 0/30 0/30

Test Example  4. According to the concentration of the herbal herbal medicine extract of the present invention DPPH  Antioxidant Effect by Scavenging Activity

Reactions by free radicals are almost always radical reactions, including the process of automatic oxidation. Antioxidants are responsible for blocking the automatic oxidation in vivo. The greater the reducing power as the electron donating ability, the stronger the antioxidant. A reagent that measures the reducing power of antioxidants is 1,1-diphenyl-2 picrylhydrazyl radical (DPPH). DPPH exists as a radical of a stabilized structure of the nitrogen center in the compound. It exhibits maximum absorption at 517 nm and is not absorbed at 517 nm when reduced. The magnitude of the reducing power of the complex herbal extract of the present invention is expressed by radical scavenging activity (SCcavenging activity, SC50) and SC50 represents the concentration required to reduce the concentration of DPPH by 50%.

DPPH (D9132, SIGMA) was dissolved in methanol. At this time, since methanol may be evaporated when the stock is a stock solution, a stock solution is prepared and dissolved in a ratio of 6: 4 with distilled water. The dissolved DPPH solution was adjusted to have an absorbance of about 0.6 at 513 nm. 2 ml of DPPH solution was added to each 1 ml of diluted sample and mixed. After standing at room temperature for about 1 hour, absorbance was measured at 513 nm using an Absorption Spectrophotometer (Uvmax kinetic microplate reader, Molecular Device), and the results are shown in Tables 5, 6, and FIG.

As shown in Figure 1 and Table 5, the extract of Example 1 of the present invention was confirmed that there is an antioxidant effect by increasing the DPPH scavenging ability in a concentration-dependent manner. In addition, as shown in Table 6, when comparing Example 1 using the ultrasonic extraction method of the present invention and Comparative Example 1 using the hot water extraction method, DPPH scavenging ability of Example 1 using the ultrasonic extraction method is shown in Comparative Example 1 It was about 45% better than that, and it was found that the DPPH scavenging ability was superior to Comparative Examples 2 to 14, which are ultrasonic extracts of the herbal medicines constituting the herbal composite material of the present invention.

Example 1 Concentration of Extract Sample (v / v%) DPPH Scavenging Activity (%) 0.2 33 0.4 72 0.8 93 1.7 93

SC 50 Ascorbic acid 2.1 μg / ml Example 1 2.7 μl / ml Comparative Example 1 3.29 μg / ml Comparative Example 2 3.99 μg / ml Comparative Example 3 3.80 μg / ml Comparative Example 4 3.78 μg / ml Comparative Example 5 3.85 μg / ml Comparative Example 6 4.05 μg / ml Comparative Example 7 4.42 μg / ml Comparative Example 8 3.55 μg / ml Comparative Example 9 3.26 μg / ml Comparative Example 10 4.01 μg / ml Comparative Example 11 3.87 μg / ml Comparative Example 12 3.54 μg / ml Comparative Example 13 4.53 μg / ml Comparative Example 14 4.61 μg / ml

Test Example  5. Hydroxyxyl according to the Concentration of Herbal Complex Medicine Extract of the Present Invention Radical  Antioxidant Effect by Scavenging Activity

Hydroxy radicals are the most potent free radicals that damage cells and cause free radical reactions of lipids in skin cell membranes, especially active oxygen species, which are major contributors to DNA and connective tissue matrix damage. Hydrogen radicals and hydrogen peroxide are produced by photosensitization reactions that begin with ultraviolet absorption, and they participate in the production of more dangerous hydroxy radicals in vivo. That is, superoxide can reduce Fe (III) and Cu (II), and reduced Fe (II) and Cu (I) reduce hydrogen peroxide and consequently generate hydroxy radicals. In vitro, hydroxy radical scavenging activity can be evaluated by measuring the ability to scaveng hydroxy radicals produced by the Fenton reaction.

The magnitude of the reducing power of the herbal complex herbal extract of the present invention can be expressed by the hydroxy radical scavenging activity (SCcavenging activity, SC50) and SC50 represents the concentration required to reduce the concentration of hydroxy radicals by 50%. The results obtained by measuring SC50 by concentration for the herbal extracts of the combination medicine of the present invention are shown in Figures 2, Tables 7 and 8.

Example 1 Concentration of Extract Sample (v / v%) Hydroxy radical scavenging activity (%) 0.5 22 5.0 65 10.0 76

SC 50 Medicine extract of Example 1 19.8 μl / ml Trolox 1.1 μg / ml

Test Example  6. According to the concentration of the herbal herbal medicine extract of the present invention Superoxide  Anion Scavenging Activity Effect

Superoxide anion among the active oxygen is produced by the photosensitization reaction that begins with ultraviolet absorption, and is converted to hydrogen peroxide by SOD (Superoxide dismutase) enzyme present in the living body. Superoxides and hydrogen peroxides are less reactive and therefore rarely directly involved in tissue cell damage, but participate in the production of more dangerous hydroxy radicals. That is, the superoxide can reduce iron and copper ions, and the reduced iron and copper ions reduce hydrogen peroxide, resulting in hydroxyl radicals. In vivo, superoxides can also release iron ions from ferritin. This phenomenon appears after ultraviolet irradiation and is considered to be the action of superoxide produced by ultraviolet light.

1.6 ml of Triton X-100 in 300 mM phosphate buffer (pH 7.8), 2.9 mg of EDTA, 1 mg of nitroblue-tetrazolium (NBT, Sigma), 5.4 mg of hypoxanthine Was added to form a substrate / reaction solution. 12 μl of xanthine oxidase was dissolved in 10 ml of 300 mM phosphate buffer (pH 7.8) to make an enzyme solution. 1.0 ml of the buffer was placed in the spectrophotometer cell, and 0.5 ml of the prepared substrate / reaction solution was added. Thereafter, 100 μl of each sample of Table 1 diluted according to the concentration was treated. 0.5 ml of the enzyme solution was added thereto, reacted at 37 ° C. for 30 minutes, and the absorbance at 540 nm was measured and shown in Tables 9 and 10.

As shown in Table 9 and Figure 3, the extract of Example 1 of the present invention was increased concentration-dependent scavenging activity for the superoxide anion. In addition, as shown in Table 10, the scavenging activity (SC50) of the superoxide radical was 4.23 µl / ml, which was superior to that of trolox, an example of a commercially available antioxidant.

Example 1 Concentration of Extract Sample (v / v%) Superoxide Anion Scavenging Activity (%) 0.17 35 0.33 44 1.67 96

SC50 Medicine extract of Example 1 4.23 μl / ml Trolox 27.2 μg / ml

Test Example  7. According to the concentration of the herbal herbal medicine extract of the present invention DNA  Protective effect

The hydroxyl radicals produced by the Fenton reaction using trace iron ions attack plasmid DNA with a supercoiled structure and turn it into nicked circular DNA. At this time, if there is a substance capable of removing the active oxygen, the plasmid DNA is no longer damaged.

According to the above theory, the DNA protection ability according to the concentration of the extract of Example 1 of the present invention was tested using pQE30 plasmid (QIAGEN, Valencia, CA, USA). 1 μg of the pQE30 plasmid was added to the Eppendorf tube, 2 μl of the sample was added to the test sphere, and the final volume was 10 μl and kept at room temperature for 10 minutes (preincubation). 10 µl of Fenton's reagent was added thereto. The reaction was carried out at 37 ° C. for 30 minutes and the reaction was stopped at −70 ° C. After electrophoresis using 0.8% agarose gel, stained with EtBr, washed with water and observed a band on the UV (UV).

As shown in Figure 4, it can be seen that the extract of Example 1 of the present invention exhibits a DNA protection effect on the active oxygen in the test zone for each concentration of lanes 3 to 6, it was confirmed that the protection effect is increased in a concentration-dependent manner. .

Test Example  8. According to the concentration of the herbal herbal medicine extract of the present invention Collagenase  Activity inhibition experiment

One of the causes of skin wrinkles is a lack of collagen, a collagen. Collagen is a major protein constituting the dermis of the skin, and plays a role in maintaining skin structure and elasticity. Collagen is known to produce less wrinkles with age as its age decreases and its degree of degradation increases, leading to depression of the dermal dermal layer. In addition, ultraviolet ray, which is the cause of photoaging, expresses matrix metalloproteinase (MMP) such as collagenase, thereby increasing the decomposition of collagen and generating wrinkles. Therefore, by checking whether the herbal extract of the present invention inhibits the collagenase activity, we tried to see the effect of improving skin wrinkles.

In the test strip, 0.05 ml of 0.05 M tricine buffer (pH 7.5), 0.05 ml of a substrate solution in which FALGPA (0.2 mM, AMS bio, UK) and 0.05 ml of sample solution were added, and collagenase (3 mg) was added thereto. / ml, Calbiochem, Germany) 0.05ml was added and left at 20 ° C for 18 hours. Thereafter, 10 µl of 1N acetate was added to stop the reaction, and the inhibitory activity was measured at absorbance of 324 nm. The inhibitory activity is shown in FIG. 5 and Table 11, and the IC50 of the collagenase is shown in Table 12 below.

As shown in Table 11 and Figure 5, the extract of Example 1 according to the present invention increased the collagenase activity inhibitory activity in a concentration-dependent manner. In addition, as shown in Table 12, collagenase inhibitory ability (IC50) was also excellent.

Example 1 Concentration of Extract Sample (v / v%) Collagenase Inhibitory Activity (%) 0.5 41 1.0 57 2.5 68 5.0 80

IC 50 Medicine extract of Example 1 6.7 μl / ml

1 is a graph showing the DPPH scavenging activity according to the concentration of the herbal herbal extract extract of the present invention,

Figure 2 is a graph showing the hydroxy radical scavenging activity according to the concentration of the herbal herbal extract extract of the present invention,

Figure 3 is a graph showing the superoxide anion scavenging activity according to the concentration of the herbal herbal extract extract of the present invention,

Figure 4 is an electrophoretic picture showing the DNA protection activity according to the concentration of the herbal extracts of the present invention, NC DNA is the abbreviation of Nicked circular DNA, SC DNA is the abbreviation of Supercoiled DNA, Lane 1 Silver test tube containing only DNA, Lane 2 is Fenton's reagent treated test lane, Lane 3 is 2 μl of Example 1 extract, Lane 4 is 1 μl of Example 1 extract, Lane 5 Is a test sphere treated with 0.2 μl of Example 1 extract, Lane 6 is a test sphere treated with 0.1 μl of Example 1 extract,

Figure 5 is a graph showing the collagenase activity inhibitory ability according to the concentration of the herbal herbal extract extract of the present invention.

Claims (6)

As a cosmetic composition containing as an active ingredient herbal herbal extract extract having antioxidant activity, The herbal complex medicine extract is Angelica gigas Nakai), peony (Paeoniae lactiflora var. trichocarpa BUNGE. ), mokdanpi (Moutau Cortex ), Hoelen Rubra , Longane Arillus ), safflower ( Carthami Flos ), Cyperi Rhizoma , Gardeniae Fructus ), mint ( Menthae Herba ), Bupleuri Radix ), Chrysanthemi Flos ), Jujube ( Zizyphi Fructus ) and a cosmetic composition, characterized in that the herbal extract of herbal composition consisting of honey. The cosmetic composition of claim 1, wherein the extract is extracted by a low temperature ultrasonic extraction method. The composition of claim 1, wherein the composition of the herbal composite material is based on 100% by weight, the total weight of the dry weight of the herbal composite material, 3-20% by weight, peony 3-20% by weight, 3-20% by weight of bark skin , 3 ~ 20% by weight, red meat 3 ~ 10% by weight, safflower 3 ~ 20% by weight, hyangbuja 3 ~ 20% by weight, gardenia 2-20% by weight, peppermint leaf 2-20% by weight, sea tiger 2-20% by weight , Chrysanthemum 2 to 20% by weight, jujube 3 to 20% by weight, honey 3 to 50% by weight of the cosmetic composition, characterized in that mixed. The cosmetic composition of claim 1, wherein the extract is extracted using at least one solvent selected from the group consisting of purified water, C ₁ to C ₄ lower alcohols, and mixtures thereof. The cosmetic composition according to claim 1, wherein the extract is contained in an amount of 0.1 to 10 wt% based on the total weight of the cosmetic composition. The method of claim 1, wherein the cosmetic composition is a water-soluble liquid, lotion, cream, gel, pack, essence, oil-in-water (O / W), water-in-oil (W / O) type and water-in-silicon (W / Si) type powder formulation Cosmetic composition, characterized in that one selected from the group consisting of.

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