KR100583792B1 - Cosmetic composition for whitening, anti-inflammation and anti-dendruff containing yellow soil and medicine herbs extract - Google Patents

Abstract

The present invention relates to a cosmetic composition having dandruff and skin whitening effect while inhibiting dermal inflammation induction by inhibiting the growth of bacteria involved in inflammation, and the cosmetic composition according to the present invention comprises yellow soil, and Gaza, fruit fruit, Daesan, It is characterized in that it comprises 18 kinds of herbal extracts consisting of large blue leaf, abdominal herb, mountain rump, yeonkyo, yongdamcho, motherwort, red tide, jimo, jingon, cheongdae, gardenia, paechocho, pogongyong, hoicho, and hobak.

Ocher, skin whitening, anti-inflammatory, anti-dandruff, herbal extract

Description

Skin whitening, anti-inflammatory and anti-dandruff cosmetic composition containing ocher water and herbal extracts {COSMETIC COMPOSITION FOR WHITENING, ANTI-INFLAMMATION AND ANTI-DENDRUFF CONTAINING YELLOW SOIL AND MEDICINE HERBS EXTRACT}

1 is a view showing the evaluation of the growth inhibitory effect on the inflammation-inducing strain of the cosmetic composition according to the present invention.

2 is a view showing the evaluation of the melanin production inhibitory efficacy of the cosmetic composition according to the present invention.

The present invention relates to a cosmetic composition, and more particularly to a cosmetic composition having an anti-inflammatory effect, a whitening effect and an anti-dandruff effect.

The skin contains Gram-positive strains Staphylococcus aureus , Staphylococcus epidermidis , Streptococcus pyogenes , Mycrococcus luteus and Corynebacterium germs. ( Corynebacterium xerosis ), gram-negative strains Escherichia coli and Pseudomonas aeruginosa , yeast Candida albicans and Malassezia furfur , and the fungus Trichophyton g . Trichophyton Mentagrophytes is present, and among them, Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus pyogenes are known to cause purulent infections, cellulitis or general inflammation on the skin. , do Cecile Jia sputter (Malassezia furfur) is Deumeul it is known to cause.

In general, to treat skin diseases such as inflammation, chemically apply vitamin A derivatives, triclosan, azelaic acid, or benzolperoxide, or tetracycline Although antimicrobial treatments such as) are used, these chemical drugs can cause a lot of irritation to the skin depending on the symptoms of the lesion. Therefore, they should be used after consultation with a specialist depending on the condition of the skin.

In addition, such an antimicrobial substance is known to cause a number of problems, such as causing the resistant bacteria, side effects such as photosensitivity. In addition, it has been reported that trichloro acetyl acid and glycolic acid, which are used in skin dermabrasion to dissolve skin keratin, cause skin sensitization or dissolve keratin to make skin appear rough.

Therefore, there is a need in the art for a new ingredient that can be used as a cosmetic raw material having excellent skin inflammation inhibitory, anti-dandruff and skin whitening effects without causing skin troubles and skin irritation.

Accordingly, the present inventors also conducted intensive research to develop a cosmetic raw material excellent in anti-inflammatory, anti-dandruff and skin whitening effects without causing skin troubles and skin irritation, yellow water, and Gaza, fruit fruit, Daesan A composition comprising 18 kinds of herbal extracts, consisting of large blue leaves, abdominal herb, wild boiled roots, ducts, gentians, motherwort, crops, balsams, pendulums, blue larvae, gardenia, paddy grass, poongongyong, hoicho It was found that the present invention can be usefully used as a cosmetic raw material, and completed the present invention.

Accordingly, it is an object of the present invention to provide a cosmetic composition excellent in anti-inflammatory, anti-dandruff and skin whitening effects without causing skin troubles and skin irritation.

In order to achieve the above object, the cosmetic composition according to the present invention, yellow soil, and let's go, fruit fruit, Daesan, Daecheongpyeon, abdominal muscle, triceps, poncho, gentian, motherwort, red tide, jinmo, Jingyo, Cheongdae, gardenia It is characterized by containing 18 kinds of herbal extracts, consisting of paddy vinegar, poongyong, hoi cho, hoobak.

Hereinafter, the present invention will be described in more detail.

There is a large amount of calcium carbonate (CaCO ₃ ) in the loess, whereby the loess has a viscous force that is not easily broken, and it turns into clay when water is added. In addition, ocher contains silica (SiO ₂ ), alumina (Al ₂ O ₃ ), iron, magnesium, sodium, and the like.

Such ocher is known to be effective in preventing aging of the human body by releasing beneficial infrared rays to the human body, activating the physiological function of the cells and generating heat energy, activating metabolism and blood circulation. In addition, it is widely known that beneficial effects on skin beauty, such as inhibiting the growth of bacteria such as mold with excellent antimicrobial properties and removing wastes with high adsorption.

In addition, there are countless microorganisms in ocher and many of the various enzymes they produce are beneficial to the human body. For example, catalase is a natural antioxidant that removes lipid peroxide, a toxin component that occurs during metabolic processes in the human body and causes aging. It is effective in preventing aging.

The present inventors also tried to find herbal medicines that can be used together with such ocher to further improve skin inflammation inhibitory, skin whitening, and antidandruff effects. As a result, among the 69 natural aromatic herbal extracts tested, Gaza, fruit fruit, and Daesan , 18 kinds of herbal medicines of large blue leaf, abdominal herb, mountain root, yeonkyo, yongdamcho, motherwort, antagonist, jimo, jinkyo, cheongdae, gardenia, pajangcho, pogongyong, hoicho, hobak Propionibacterium acnes , gram-positive strains Staphylococcus aureus , Staphylococcus epidermidis , Streptococcus pyogenes , gram-negative bacteria Pseudomonas master ah wool group was found to have excellent antimicrobial activity with respect to labor (Pseudomonas aeruginosa), in particular to the whitening effect it is excellent in a second Hoi It was.

According to the present invention, when the yellow soil water and 18 natural herbal extracts are mixed in a weight ratio of 1: 0.5, the most excellent skin whitening, anti-inflammatory and anti-dandruff effects are shown.

Here, the ocher water means a mixture of water: ocher in a weight ratio (v / w) of 1: 0.01 to 0.5, and the water used to prepare such ocher water includes general water, tap water, bottled water, distilled water. , Saline, deep water, and the like.

The herbal extracts contained in the composition according to the present invention may be prepared by mixing all 18 kinds of herbal medicines and then performing extraction by any method known in the art, or extracting each herbal medicine to obtain each extract obtained. It can be prepared by mixing each in an appropriate ratio. For example, it can manufacture as described in manufacture example 2. On the other hand, when the extraction is performed after mixing all 18 herbal medicines, the ratio of mixing each herbal medicine is not particularly limited, for example, 0.01 to 20% by weight of each herbal medicine relative to the total weight of the total mixture Although it may be mixed in the range of%, it will be apparent to those skilled in the art that anything other than the above range may be used.

According to the present invention, the content of the herbal extract in the cosmetic composition is 0.01 to 20% by weight, preferably 0.1 to 10% by weight relative to the total weight of the total cosmetic composition. If the content of the herbal extract is 0.01% by weight or less, it is difficult to obtain a whitening or anti-inflammatory and anti-dandruff effect, and if it is 20% by weight or more, an increase in the anti-inflammatory effect is observed, but it is uneconomical in terms of the stability of the product.

The mixing ratio of the yellow soil water: the herbal medicine extract is in a weight ratio of from 1: 0.01 to 1: 20, preferably from 1: 0.1 to 1: 10, and the total content of the yellow soil water and the herbal medicine extract is based on the total weight of the total cosmetic composition. 0.01 to 20% by weight, preferably 0.1 to 10% by weight.

The cosmetic composition according to the present invention is not particularly limited in the formulation, for example, creams, essences, soaps, shampoos, rinses, aisors, mascara, powders, creams, night creams, lotions, skin care, eye creams, It will be apparent to those skilled in the art that lipsticks and the like can be formulated into a variety of other formulations known in the art.

In addition, the composition according to the present invention can also be used as a cosmetic composition having an acne prevention and treatment effect.

In addition, those skilled in the art will be apparent to those skilled in the art for the formulation and the type and amount of ingredients to be added thereto.

Hereinafter, although an Example demonstrates this invention in detail, this invention is not limited by this.

EXAMPLE

<Manufacture example 1> Manufacture of yellow soil

First, ocher powder having a particle size of 5,000 to 7,000 mesh was prepared as follows.

The yellow soil collected from Naju Phoenix and Yeongam New North Korea was kept in natural state for more than a month to mature the ocher, and the matured ocher was crushed by an air flow type grinder with strong rotational force. When the pulverization is completed, the air discharged from the pulverizer and the pulverized ocher particles are separated according to the particle size difference of the ocher powder by the blower wind of the classifier, where only 5,000 to 7,000 mesh fines were selected.

In the preparation of the cosmetic composition according to the present invention, it is preferable to use the ocher of the fine powder selected as described above without further processing, so that the effect of many beneficial microorganisms present in the ocher is not impaired by using the ocher in its natural state. To do this. This powder was mixed with commercial deep sea water at a weight ratio (v / w) of 1: 0.01 to 0.5 to prepare ocher water and used in the present invention.

Preparation Example 2 Preparation of Herbal Medicine Extract

First, 1,3BG (Butylene Glycol) and ethanol were mixed in the same mass ratio and stirred to form a single phase, which was used as a cosolvent in an extraction process using supercritical carbon dioxide. 18 kinds of herbal medicines containing the same amount of each herbal medicine (Gaza, fruit fruit, Daesan, Daecheongyeop, abdominal herb, Sandugeun, Yeongyo, Yongdamcho, motherwort, red rice, Jimo, Jinkyo, Cheongdae, Gardenia, Pajangcho, Poongcho, Hoicho, thick foil, herbal medicines below) was dried until there is no change in weight, and then ground to a size of 0.3 cm or less using a grinder. The pulverized herbal medicine and cosolvent were mixed at a mass ratio of 1: 2, stirred for 30 minutes, and then the mixture of the herbal medicine and the cosolvent was put into a supercritical extraction tank having an internal volume of 1.5 L and sealed, and a carbon dioxide was obtained by using a high pressure gas pump. The pressure was increased by supplying. The temperature inside the extraction tank was maintained for 50 minutes while raising the temperature to 40 ° C at a rate of 0.5 ° C / min. Again, while depressurizing to atmospheric pressure while releasing the extract while supplying carbon dioxide, the carbon dioxide and the herbal extracts were separated, wherein carbon dioxide was released into the atmosphere. Meanwhile, the herbal extracts were collected in a collector and used in the present invention.

<Example 1>

The ratio of the herbal extract (preparation example 2): ocher water (preparation example 1) was mixed in a weight ratio of 0.01: 1.

<Example 2>

The ratio of the herbal extract (preparation example 2): ocher water (preparation example 1) was mixed in a weight ratio of 0.1: 1.

<Example 3>

The ratio of the herbal extract (preparation example 2): ocher water (preparation example 1) was mixed in a weight ratio of 0.5: 1.

<Example 4>

The ratio of the herbal extract (preparation example 2): ocher water (preparation example 1) was mixed in a weight ratio of 1.0: 1.

Example 5

The ratio of the herbal extract (preparation example 2): ocher water (preparation example 1) was mixed in a weight ratio of 10.0: 1.

Comparative Example 1

The herbal extract (Production Example 2) was used as it was, and the same amount of ordinary purified water was mixed instead of yellow soil water.

<Formulation Example 1-3, Comparative Example 1-2>

Preparation of Cosmetics Containing Mixtures of Yellow Water and Herbal Extracts

The mixture of Example 2, the mixture of Example 3, the mixture of Example 4, according to the composition of Table 1, according to the composition of Table 1, using a composition commonly used in the industry according to the formulation Each was mixed and stirred with a homomixer, and then heated and cooled to prepare a cosmetic composition of Formulation Examples 1 to 3 uniformly mixed. Further, using the mixture of Comparative Example 1, a cosmetic of Comparative Example 1 was prepared in the same composition as in Production Examples 1 to 3.

Raw material name Formulation Example 1 Formulation Example 2 Formulation Example 3 Comparative Example 1 Comparative Example 2 Poly Solate-60 1.5 1.5 1.5 1.5 1.5 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 Stearic acid 1.0 1.0 1.0 1.0 1.0 Squalane 5.0 5.0 5.0 5.0 5.0 Cerro stearyl alcohol 2.0 2.0 2.0 2.0 2.0 Seryl Octanoate 5.0 5.0 5.0 5.0 5.0 Carbomer 0.15 0.15 0.15 0.15 0.15 Triethanolamine 0.2 0.2 0.2 0.2 0.2 glycerin 5.0 5.0 5.0 5.0 5.0 Mixture of Example 2 2.0 Mixture of Example 3 2.0 Mixture of Example 4 2.0 Extract of Comparative Example 1 2.0 antiseptic Quantity Quantity Quantity Quantity Quantity Purified water Total 100 Total 100 Total 100 Total 100 Total 100

Experimental Example 1 Inflammation-Reducing Effect

Richard P.G., Alan J.L. et al., Agents and Actions, Vol. As described in 17, 2, 1985, after the mice were inflamed with arachidonic acid, the degree of inhibition of inflammation was measured by applying ocher water to the mouse ears and measuring the ear thickness. Specifically, a mixture of Examples 1 to 5 containing the above yellow soil water was applied to the left ear of both ears of 10 mice, and Comparative Example 1 was applied to the right ear. After 30 minutes, arachidonic acid (1 μg / 20 ml) was applied, and after 1 hour, the thickness of both ears was repeatedly measured five times with a micrometer to calculate the degree of edema. The results are shown in Table 2 below.

Ear thickness (㎛) Before arachidonic acid treatment After arachidonic acid treatment Example 1 320 ± 9 448 ± 14 Example 2 325 ± 5 407 ± 9 Example 3 328 ± 11 387 ± 6 Example 4 347 ± 14 364 ± 8 Example 5 331 ± 7 354 ± 7 Comparative Example 1 315 ± 8 546 ± 17

As shown in Table 2, the composition according to the present invention comprising ocher water and herbal extracts inhibited the inflammatory response in mice.

<Experimental Example 2> Effect of preventing the growth of harmful bacteria on the skin of the composition according to the present invention

In this experimental example, the effect of preventing the harmful growth of the skin of the conventional cosmetic components and the composition according to the present invention was compared. Specifically, conventional cosmetic ingredients include salicylic acid, rovisome acnes, phycosaccharide antiacne, triclosan, and azelaic acid, which are known to have acne and skin inflammation inhibitory effects. And phytosphingoshine (Phytosphingoshine) and the effect of the cosmetic composition according to the present invention was compared.

As an experimental method, each of the bacteria shown in Table 3 was plated at 1 × 10 ⁷ ea / ml on a solid flat medium made of Tryptic soy agar (TSA) (Difco co.), Followed by 20 μl of each sample shown in was dropped and air dried. Each sample was diluted 100-fold and used at a concentration of 10 g / l. After natural drying, the cells were incubated at 37 ° C. for 24 hours to determine the presence or absence of transparent low-ring rings.

The results are shown in Table 3, Table 4 and FIG.

  No Target substance S. Aureus (ATCC 65389) S. Epidermis (ATCC 12228) S. Piogens (ATCC 21059) P. Aknes (ATCC 6919) Pseudomonas. Aeruzinosa One Phenyl ethyl alcohol +/- + - - +/- 2 Citronellol - +/- - +/- - 3 Geraniol +/- - +/- - +/- 4 α-ionone - +/- - +/- - 5 Furan - - +/- - - 6 γ-dodecaractone +/- - - - - 7 γ-heptalactone - +/- - +/- + 8 vanillin +/- - +/- - +/- 9 γ-undecalactone - +/- - +/- - 10 β-pinene - - +/- - +/- 11 5-methyl furfural +/- - - - - 12 γ-hexalactone - +/- - +/- - 13 α-pinene +/- - +/- - +/- 14 5- (hydroxymethyl) -2-furfural - +/- - +/- - 15 2-furancarboxylic acid - +/- +/- - +/- 16 Geraniol - - - - - 17 Nerol + +/- - - - 18 Cyclotene - - - + - 19 Linalule - - + - + 20 Dehydro coumarin - - - - - 21 Mixture of Example 2 -/ + + -/ + - - 22 Geranium oil + - - - - 23 Chamomile oil - + - - - 24 Lavender oil - -/ + - - + 25 Sandalwood oil - - - + - 26 Angelica seed oil + - + - - 27 Fine Needle Oil - -/ + - - - 28 Sandalwood oil -/ + - - - - 29 Ylang-ylang oil - + - - - 30 Juniper Berry Oil + - - + - 31 Rosemary oil - - -/ + - - 32 Mixture of Example 1 -/ + + + -/ + -/ + 33 Mixture of Example 3 ++++ ++++ ++++ +++ +++ 34 Salicylic acid + - - +/- - 35 Robby Moth - - - + - 36 Pico saccharit anthiakne +/- - - + - 37 Triclosan ++ + ++ +++ +/- 38 Azelaic acid - +/- -      + - 39 Phytosphingosine + + - + -  -: Can not produce low ring, +/-: Strain growth after low ring generation, +, ++: Weak low ring, +++, ++++: Strong low ring

M. Purpur P. Obale Plate # Diameter of the ring (cm) Lowlander Plate # Diameter of the ring (cm) Lowlander Formulation Example 2 #One 3.0 ++ #One 2.0 ++ #2 2.7 ++ #2 2.0 ++ Comparative Example 1 #One 3.0 +++ #One 3.0 +++ #2 3.0 +++ #2 2.4 ++ C blank #One 1.6 + #One 1.6 + #2 2.0 + #2 1.4 +

As can be seen from the results of Table 3 and Table 4, conventional raw materials affect some of the five strains, among which triclosan showed the strongest effect, but the latest news (Christopher Mack, University of Minnesota, USA). According to Dr. Nail: 2002-4-17, Health / Yonhap News, Triclosan is not suitable for use as a cosmetic ingredient because it is converted to toxic dioxins when exposed to sunlight.

On the other hand, as can be seen from the results of Figure 1, when treated with a cosmetic containing a mixture of ocher water and herbal extracts produced a strong low-ring ring in all five strains.

Therefore, it can be seen that the composition according to the present invention has an excellent effect compared to the conventional materials used in cosmetics in the anti-dandruff effect and anti-dandruff effect by dermatophytes. In addition, since the composition according to the present invention uses the herbal extract as a raw material, there is no skin irritation or side effects.

Experimental Example 3

Growth Inhibitory Effect on Dandruff-inducing and Skin Reproductive Bacteria

In order to measure the growth inhibitory effect on the dandruff-inducing bacteria and dermatophyte bacteria of the cosmetic preparations prepared in Formulation Examples 1-3 and Comparative Example 1, in Table 5a to 5e The five strains shown were incubated.

After measuring the initial number of inoculated bacteria, 0.5 g of each of the prepared cosmetics was added to each medium and placed on a culture dish, and five strains (2.1 × 10 ⁷ ea / ml) in which the bacterial counts were measured beforehand were inoculated. The male seed was incubated for 3 days in an anaerobic incubator, and the remaining four strains were incubated for 3 days in a general incubator to determine the number of strains. The results are shown in Tables 5a to 5e.

Growth Inhibitory Effect on Male Pupu 0 days 3 days Inhibitory ability (%) Formulation Example 1 2.30E + 07 3.89E + 06 83.08696 Formulation Example 2 2.30E + 07 1.12E + 02 99.99951 Formulation Example 3 2.30E + 07 3.27E + 06 85.78261 Comparative Example 1 2.30E + 07 1.12E + 07 51.30435

Growth Inhibitory Effect on Pseudomonas auruginosa 0 days 3 days Inhibitory ability (%) Formulation Example 1 2.30E + 07 3.99E + 06 82.65217 Formulation Example 2 2.30E + 07 1.32E + 02 99.99943 Formulation Example 3 2.30E + 07 3.02E + 06 86.86957 Comparative Example 1 2.30E + 07 1.42E + 07 38.26087

Growth Inhibitory Effect on Streptococcus pyogens 0 days 3 days Inhibitory ability (%) Formulation Example 1 2.30E + 07 3.46E + 06 84.95652 Formulation Example 2 2.30E + 07 9.80E + 01 99.99957 Formulation Example 3 2.30E + 07 2.85E + 06 87.6087 Comparative Example 1 2.30E + 07 1.25E + 07 45.65217

Growth Inhibitory Effect on Streptococcus epidermidis 0 days 3 days Inhibitory ability (%) Formulation Example 1 2.30E + 07 2.96E + 06 87.13043 Formulation Example 2 2.30E + 07 1.42E + 02 99.99938 Formulation Example 3 2.30E + 07 2.45E + 06 89.34783 Comparative Example 1 2.30E + 07 1.44E + 07 37.3913

Growth Inhibitory Effect on Staphylococcus aureus 0 days 3 days Inhibitory ability (%) Formulation Example 1 2.30E + 07 2.26E + 06 90.17391 Formulation Example 2 2.30E + 07 1.02E + 02 99.99956 Formulation Example 3 2.30E + 07 1.85E + 06 91.95652 Comparative Example 1 2.30E + 07 1.12E + 07 51.30435

As can be seen from the results of Tables 5a to 5e, it is possible to confirm the excellent growth inhibitory ability of the cosmetic containing the ocher water and herbal extracts in Formulation Examples 1-3, Formulation Example 2 for Formulation Example 1 and Formulation Example 3 It was confirmed that the overall inhibitory capacity is relatively low. In addition, when comparing the Formulation Example 2 and Comparative Example 1, the growth inhibitory ability showed a great difference depending on the presence or absence of yellow soil water.

Experimental Example 4

Human patch experiment

In order to confirm that the cosmetic composition containing the yellow ocher water and the herbal extract has no skin irritation and other side effects, a patch test was performed on the human body in the cream formulations of Comparative Example 1 and Formulation Example 2.

The test consists of 30 healthy males and females who use a Finn chamber on the back to put cosmetic products directly into aluminum discs or apply 40 μl of paper discs to the skin using a scanpore tape. It fixed and the patch was removed after 24 hours, and the result was determined after 4 hours.

According to the following criteria (Wooding et al, 1967; Rietschell, 1982; Fischer & Maibach, 1984; Aberer et al, 1993) of the International Contact Dermatitis Research Group (ICDRG), the results of erythema and edema Numerical values are indicated as follows:

0: no redness

1: erythema

2: redness and papules

3: redness, papules and vesicles

4: severe edema and vesicles

Based on the score determined according to the above criteria, the degree of stimulation was calculated according to the following equation, and the results are shown in Table 6 below.

Stimulus (%) = (Reactivity indicating positive response / Responsiveness of self-population) × 100

Comparative Example 1 Formulation Example 2 Irritation degree (%) 5.0 0.01

As can be seen from the results of Table 6, the cosmetic composition according to the present invention can be seen that there is no dermatitis or other irritation.

Experimental Example 5

Animal Clinical Trials of Cosmetics Containing Yellow Water and Herbal Extracts

Using Formulation Example 2 and Comparative Example 1, animal clinical effects experiments on dandruff and general inflammation were performed.

Hairless mouse (hereinafter referred to as "experimental animal") (6 weeks old, female) 10 compartments of the back of the two compartments and arachidonic acid (Arachidonic acid; manufactured by Sigma) initially observed inflammation after visual observation, Four strains 2 × 10 ⁵ ea were applied in cumulative amounts of 200 μl at intervals of 2-3 days to construct a dandruff and prone animal model.

The sample of Formulation Example 2 was applied to the left skin of the constitutive model, and the sample of Comparative Example 1 was applied to the right skin at 2 mg / cm 2 daily until dandruff and inflammation were induced. When dandruff and inflammation were induced, an abscess was collected from the affected area and cultured to identify the same strain.

In the affected area caused by the same strain as the strain used in the present invention, the cream-like cosmetic was applied to the left skin by applying the sample of Formulation Example 2 on the right side and the sample of Comparative Example 1 on the right every day at 2 mg / cm 2 for one month. While observing, the effect was measured.

As for the determination of the effect of this clinical trial, a standard was set as shown in Table 7 below, and 2-3 observers determined a score or grade for the criterion and performed relative comparison.

score Affected state effect One Healing normally Great 2 The affected area is intermediate or no longer in progress usually 3 The affected area is larger or more inflamed none

According to the criteria of Table 7, the results of visual determination of the affected part of the experimental animals after one month are shown in Table 8 as the number of animal models.

Formulation / Effect Excellent effect Effect usually no effect system Formulation Example 2 8 2 - 10 Comparative Example 1 - One 9 10

As shown in the results of Table 8, 80% of the 10 experimental animals showed an excellent effect in the formulation of Formulation Example 2, 90% of Comparative Example 1 was found to be ineffective.

Therefore, as a result of clinical experiments on the affected areas of dandruff and inflammation, it can be seen that the anti-dandruff and inflammation-releasing effect is excellent in cosmetics to which ocher water and herbal extracts are added.

Experimental Example 6

Tyrosinase Inhibition Test

Tyrosinase inhibitory effect of the cosmetics containing yellow water and herbal medicine used in Formulation Example 2 and arbutin, which is a whitening raw material conventionally used in cosmetics, was measured.

The enzyme tyrosinase was extracted from mushrooms, and SIGMA T7755's was used. First, prepare a tyrosine substrate as a 0.3 mg / ml solution in distilled water, add 1.0 ml of this solution to a test tube, and add 1.0 ml of potassium phosphate buffer (0.1M, pH 6.8) and Formulation Example 2. 0.9 ml of each dilution solution was added to the reaction solution and reacted for 10 minutes in a 37 ° C thermostat. Ethanol and water were used as a diluting solvent at 6: 4, and 0.9 ml of the comparative raw material was used instead of Formulation Example 2 as a control. 0.1 ml of 2,500 units / m of tyrosinase solution was added to the reaction solution, and the reaction solution was reacted for 10 minutes in a 37 ° C thermostat. Based on the following formula, each extract was evaluated for tyrosinase inhibitory effect.

Tyrosinase Inhibition Rate (%) = 100-(Extract Reaction Absorbance / Control Absorbance) × 100

Tyrosinase inhibition rate of Formulation Example 2 is 97.67% at the experimental concentration of 0.1% as shown in Table 9.

           Category Concentration (%) Tyrosinase inhibition rate (%) Formulation Example 2 Arbutin 10 57.21 64.21 One 78.56 67.24 0.1 97.67 24.31 0.01 92.14 30.54 0.001 87.21 24.18 0.0001 60.11 12.54

Experimental Example 7

Melanin production inhibition experiment

As a further study on the tyrosinase inhibitory activity of the cosmetic according to the present invention shown in Experimental Example 6, it was tested whether the composition according to the present invention has a melanin synthesis amount inhibitory effect. Specifically, human melanocytes isolated from human tissues were cultured first, and after two passages, cultured using RPMI 1640 medium at a concentration of 2 × 10 ⁴ / dish in a 60 mm culture dish. After confirming that the cells adhered to the dish, Formulation Example 2 was treated by concentration up to 10ng ~ 10㎛. After treatment of Formulation Example 2 by concentration, the cells were detached with trypsin-EDTA on the third day, and 2 × 10 ⁵ cells were uniformly distributed. Then, 100 μl of 1N NaOH solution was added and treated at 37 ° C. for 12 hours. Completely dissolved. The absorbance of 500 μl of samples in which cells were completely lysed was measured at 490 nm.

As a standard indicator, 10 mg of synthetic melanin (M8601) from Sigma was dissolved in 10 ml of 1N NaOH to prepare a stock solution (1 mg / ml), followed by 700 µg / ml, 300 µg / ml, 100 µg / ml, and 70 µg / ml. ㎖, 30 μg / ml, 10 μg / ml, 7 μg / ml, 3 μg / ml, 1 μg / ml, 0.1 μg / ml, 0 μg / ml and the absorbance at 490 nm was measured. 10 and FIG. 2.

Formulation Example 2 Melanin Reduction (%) One 22.017 0.1 24.1818 0.01 16.5804 0.001 15.8916 0.0001 10.3566

As shown in Table 10 and FIG. 2, the composition of Formulation Example 2 reduced the amount of melanin synthesis up to 20% at 0.1%.

 As described above, the cosmetic composition according to the present invention containing the ocher water and herbal extracts, inhibits the growth of the skin inflammation by inhibiting the growth of bacteria involved in the inflammation, while having excellent dandruff and skin whitening effect, skin irritation There are no side effects such as, and can be used as a cosmetic and skin whitening cosmetics for inhibiting dermatitis and dandruff induction, and can also have a natural antiseptic effect by increasing the long-term preservation of the cosmetic against microbial contamination.

Claims (3)

Ingredients of Ocher water prepared by mixing water: ocher in a weight ratio (v / w) of 1: 0.01 to 0.5; And 18 kinds of herbal extracts consisting of Gaza, Fruits, Daesan, Daecheongyeop, Fuyang, Shandogeun, Yeongyo, Yongdamcho, Motherwort, Jeonjak, Jimo, Jingyo, Cheongdae, Gardenia, Paechocho, Poongyong, Hoicho, Thick pepper; To include, wherein the herbal extract is contained in an amount of 0.01 to 20% by weight based on the total weight of the composition, the ratio of ocher water: herbal extracts is 1: 0.01 to 1:20 cosmetic composition for skin whitening. Ingredients of Ocher water prepared by mixing water: ocher in a weight ratio (v / w) of 1: 0.01 to 0.5; And 18 kinds of herbal extracts consisting of Gaza, Fruits, Daesan, Daecheongyeop, Fuyang, Shando-geun, Yeongyo, Yongdamcho, Motherwort, Jeokjak, Jimo, Jingyo, Cheongdae, Gardenia, Pajangcho, Poongyong, Hoicho To include, wherein the herbal extract is contained in an amount of 0.01 to 20% by weight based on the total weight of the composition, the ratio of ocher water: herbal extracts is 1: 0.01 to 1:20 cosmetic composition for inhibiting skin inflammation, characterized in that. Ingredients of Ocher water prepared by mixing water: ocher in a weight ratio (v / w) of 1: 0.01 to 0.5; And 18 kinds of herbal extracts consisting of Gaza, Fruits, Daesan, Daecheongyeop, Fuyang, Shandogeun, Yeongyo, Yongdamcho, Motherwort, Jeonjak, Jimo, Jingyo, Cheongdae, Gardenia, Paechocho, Poongyong, Hoicho, Thick pepper; It includes, the herbal extract is contained in an amount of 0.01 to 20% by weight based on the total weight of the composition, the ratio of ocher water: herbal extracts is anti-dandruff cosmetic composition, characterized in that 1: 0.01 to 1:20.

Images