KR20160021677A - Cosmetic Compositions for Scalp Containing Forsythia viridissima - Google Patents

Abstract

The present invention relates to a cosmetic composition for a scalp, containing a Forsythia viridissima extract. The cosmetic composition of the present invention contains lyophilisate of an ethanol extract of Forsythia viridissima, especially an ethyl acetate fraction of Forsythia viridissima ethanol extract. According to the present invention, the cosmetic composition for the scalp, containing the ethanol extract of Forsythia viridissima or the ethyl acetate fraction thereof safely and effectively suppresses the proliferation of dandruff bacillus owing to excellent anti-dandruff properties and antioxidative and anti-inflmmatory activities of Forsythia viridissima. In addition, the cosmetic composition of the present invention can inhibit, owing to the antioxidative activities, the production of free fatty acids produced by decomposition of sebum, thereby exhibiting effects of relieving an itchy scalp and preventing the dandruff production caused by hyperkeratinization of keratinocytes. The symptoms of inflammation and red spots are also relived by the anti-inflammatory activities as well.

Description

[0001] Cosmetic Compositions for Scalp Containing Forsythia viridissima [0002]

More particularly, the present invention relates to a cosmetic composition for scalp which contains an extract of Yeast Extract having an excellent antioxidative effect, a dandruff controlling ability and an anti-inflammatory effect, especially a specific solvent fraction extract thereof .

 In recent years, as the level of living has improved through industrialization, the interest in health and skin beauty has increased, and industrial importance for the development of new materials has been emphasized along with the development of the flourishing industry, and new materials and new type research and development are becoming active.

Particularly, as a research material for a flour using natural materials, the raw material of a natural ingredient replacing a chemical substance is getting popular, and development using various natural materials is actively being carried out.

On the other hand, dandruff in the scalp is mainly caused by blanching of the rice bran in the scalp and facial area, resulting from the removal of the stratum corneum and is considered as a mild symptom of psoriasis or dermatitis (Ackerman & Kligmn, 1969). However, when these symptoms become severe, they are called dandruff, clinically, tofu, and medically dry boredom.

If the scalp is accompanied by severe pain, itching, or inflammation or erythema, it is a symptom that is caused by direct pathogenesis of dandruff. Therefore, in order to relieve or alleviate these symptoms, the growth of dandruff must be suppressed. It is important to note that the hair growing in the hair follicle tends to be tapered and alopecia may be induced (Lee, Sung-Hyun, 2007; Roberts, 1969).

The cause of worsening of dandruff is known as cold and dry climate, excessive sebum secretion, change of sebum composition, activation and proliferation of yeast such as Malassezia furfur . Malassezia furfur , a type of yeast (fungus), begins to reside in the same bore area as scalp after puberty, and accounts for about 46% of the survivors of the boredom of normal people, and is affected by environmental factors such as climate, sweat, It is known that dandruff occurs when it is over 74%, and seborrheic dermatitis occurs when it exceeds 83% (Sukwoo, 2004).

Drugs and detergents used in the treatment of dandruff include, but are not limited to, zincpyrithione, piroctoenolamine, ketoconzole, sulfurous, tar, selenium sulfide, and the like. The chemical is contained as an antimicrobial agent. Because all of these antifungal agents exhibit antifungal effects due to bacteriostatic action, long-term administration is required, and side effects such as hepatic and renal toxicity induction, headache and skin hypersensitivity appear, and there is a problem that they tend to recur after treatment is completed 2010).

The Forsythia viridissima associated with the present invention is the fruit of the forsythia (Forsythia viridissima L.) belonging to the Oleaceae family.

The forsythia is a deciduous shrub with a height of 2 ~ 3m, branches are thin and long, spread or fall down, hollow inside, flowers bloom before leaves, 1 ~ 4 flowers are mixed on leaves, fruit is narrow ovate, It has a beak, and when fully cooked, it divides into two pieces. It is mainly grown in the wastelands of Sanya and is harvested when it is picked and used in the sun. The active ingredient of the allium is actiginin as a lignan, arctin as a lignan glycoside, and caffeic acid glycoside It is widely used as a remedy for swelling, gonorrhea, diarrhea, diarrhea, hemorrhoids, tuberculosis, leprosy, asthma, and detoxification in both oriental medicine and private medicine.

Conventionally, various kinds of cosmetics including a linkage have been proposed. However, it has been proposed to use the anti-dandruff inhibiting ability and antioxidant and anti-inflammatory activity of a linkage to safely and effectively inhibit the growth of dandruff, The present invention provides a cosmetic composition for scalp which is capable of alleviating inflammation and erythema symptoms by anti-inflammatory activity, exhibiting dandruff occurrence and scarring itching-removing effect by exfoliation of keratinocyte by inhibiting formation of free fatty acid, There is no suggestion as long as it does.

Open Patent Publication No. 10-2000-0045573 (published on July 25, 2000) Patent Registration No. 10-0560304 (registered on March 6, 2006) Patent Registration No. 10-0583792 (registered on May 19, 2006) Published Japanese Patent Application No. 10-2009-0117448 (November 12, 2009) Open Patent Publication No. 10-2010-0087785 (published on Aug. 6, 2010) Patent Registration No. 10-1026873 (registered on March 28, 2011)

As a result of various and extensive studies to select natural extracts having excellent dandruff inhibitory ability, antioxidant and anti-inflammatory effect among various kinds of domestic plants and to select an effective fraction extract by a specific solvent, Ethanol extract and specific solvent fractions thereof, thereby completing the present invention.

Accordingly, an object of the present invention is to provide a cosmetic composition for scalp which has excellent anti-dandruff, antioxidant and anti-inflammatory activity.

According to a preferred embodiment of the present invention for achieving the object of the present invention, a cosmetic composition for scalp containing a lyophilized product of ethanol extract of Forsythia viridissima is provided.

The lyophilized product of the ethanol extract of the above-mentioned tandem may be a lyophilized product of the ethyl acetate extract fraction of the above ethanol extract.

The lyophilized product may be obtained by concentrating and freeze-drying the filter extract of the ethanol extract (1: 5 to 15 w / w) of the alumina.

The ethanol-extracted freeze-dried product of the above-described tandem bridge may be contained in an amount of 0.01 to 50.0% by weight based on the total weight of the composition.

The formulation of the cosmetic composition may also be a scalp serum, hair essence, shampoo, or scalp tonic.

The cosmetic composition for scalp containing the ethanol extract of the alcohols according to the present invention or the ethylacetate fraction thereof can safely and effectively inhibit the growth of the dandruff by utilizing the excellent antidandruff inhibitory ability and antioxidant and anti-inflammatory activity of the alum In addition, the antioxidant activity inhibits the production of free fatty acid by decomposition of the sebum component to thereby exacerbate dandruff and scarring by the exfoliation of keratinocytes, and relieve inflammation and erythema symptoms by anti-inflammatory activity.

FIG. 1 is a graph showing the electron donating ability of each fraction of the ethanol extract of Kangyeol.
FIG. 2 is a graph showing the cytotoxicity of fractionated ethanol extracts of each fraction. FIG.
FIG. 3 is a graph showing the inhibitory effect of nitric oxide (NO) production on the fraction of ethanol extract of tandem suspension.

Hereinafter, the present invention will be described in detail.

The cosmetic composition for scalp according to the present invention contains a lyophilized product of ethanol extract of Forsythia viridissima and exhibits excellent anti-dandruff, antioxidant and anti-inflammatory effects, and is natural product, so there is no fear of side effects.

The lyophilized product may be one obtained by filtering an extract using filter paper as an ethanol extract (1: 5 to 15 w / v) of a tandem suspension, concentrating the filtrate, and lyophilized.

The lyophilized product of the ethanol extract of the above-mentioned tandem may be an ethyl acetate extract fraction of the ethanol extract, and the ethyl acetate fraction may be concentrated and lyophilized.

The ethanol extract or the ethyl acetate fraction thereof may be used as it is or may be used in the form of an extract which is concentrated after filtration, or may be used as a form of a lyophilized product after concentration and freeze-drying. Preferably, the extract obtained by concentration after filtration can be used as a lyophilized product in the form of a lyophilized product.

The ethanol-extracted freeze-dried product of the above-described tandem bridge may be contained in an amount of 0.01 to 50.0% by weight based on the total weight of the composition.

The formulation of the cosmetic composition may also be a scalp serum, hair essence, shampoo, or scalp tonic.

Hereinafter, the present invention will be described more specifically with reference to preferred embodiments, experimental examples, and formulation examples. However, it is to be understood that the present invention is not limited thereto.

Example 1: Preparation of Ethanol Extract of Ankle

The extracts were repeatedly extracted twice with ethanol 10 times by weight in 100 g of the dry sample of the allium purchased from Jeonnam Agricultural Products Agricultural Cooperative (Hwasun, Korea), and the extract was filtered using a filter paper (Whatman No. 2) The mixture was concentrated under reduced pressure using a rotary evaporator and lyophilized to obtain 15.4 g of an ethanol extract.

Example 2 Preparation of Fraction of Ethanol Extract of Ankyo

The ethanol extracts prepared in Example 1 were prepared by suspending 0.3 liter of distilled water in the ethanol extract, adding the same quantity of hexane as the distilled water, shaking, leaving the solvent layer concentrated under reduced pressure, and lyophilized to obtain hexane fraction .

The remaining water-soluble layers were sequentially fractionated by the addition of ethyl acetate and butanol, and then concentrated to give ethyl acetate and butanol fractions, respectively. The remaining water-soluble layer was concentrated to a water fraction. The yields of the obtained fractions are shown in Table 1 below.

All fractions were stored at -20 ° C.

The extraction yield of each solvent fraction obtained from the bridged dry weight of 100 g Year Dry weight (g) yield(%) Hexane 1.4 9.1 EtOAc 3.5 22.7 BuOH 4.6 29.9 water 6.3 40.9

               Dry weight (g) of the extracted fraction

Yield (%) = --------------------------------- x 100

Dry weight (g)

Experimental Example 1: Measurement of electron donating ability according to fractions

The electron donating ability of the fraction obtained in Example 2 was measured by the DPPH method and the fractional radical scavenging effect was measured by the Blois method (Blois, 1958). 100 μM of DPPH solution and 100 μl of each concentration fraction were mixed and left for 30 minutes in a dark state. The residual radical concentration was measured at 517 nm using a microplate reader (Molecular Devices, USA). Vitamin C and BHT, which are well known antioxidants.

The oxidation reaction in the body is mostly caused by active oxygen, and the autoxidation reaction of free radicals proceeds. The autoxidation reaction of free radicals is a chain reaction, and the reaction is terminated by receiving hydrogen peroxide as a chain reaction. Therefore, the ability of antioxidants to give hydrogen in autoxidation reactions. That is, the reducing power must be large.

DPPH is most widely used to measure the reducing power of such antioxidants, and the electron donating ability of fractions by solvent in the crosslinked copolymer is measured.

In the 500 ㎍ / ㎖ of EtOAc fraction, 93.14% was the highest, BuOH and water were 90.02% and 81.30%, respectively. Hexane showed the lowest effect at 17.90%.

The EtOAc fraction contained a large amount of phenolic compounds and was presumed to have a high electron donating ability due to the action of these compounds.

Experimental Example 2: Determination of Polyphenol and Flavonoid Content by Fractionation

Total polyphenol content was determined by Folin-Denis method, and 10 g of methanol was added to 0.1 g of the fraction, which was then extracted at 70 ° C for 30 minutes and then diluted to 1 mg / ml (Gutfinger, 1981). Add 650 μl of distilled water to 50 μl of the sample solution, add 50 μl of Folin-Denis reagent and react at room temperature for 3 minutes, add 100 μl of 10% Na ₂ CO ₃ saturated solution, adjust the final volume to 1 ml 150 μl of distilled water was added thereto and mixed well. After reacting in a 37 ° C water bath for 1 hour, the absorbance was measured at 725 nm. The standard curve was tannic acid (Sigma).

Total flavonoid content was determined by the method of Davis et al . (Swaim et al, 1959) by adding 10 ml of methanol to 0.1 g of the fraction and extracting it at 70 ° C for 30 minutes and then diluting to 1 mg / ml. 1 ml of diethylene glycol was added to 100 μl of the test solution, 100 μl of 1N NaOH was added, and the mixture was reacted in a 37 ° C water bath for 1 hour. The absorbance was measured at 420 nm. The standard curve was naringin (Sigma).

Phenolic compounds are one of the secondary metabolites widely distributed in the vegetable field and have various structures and molecular weights. Since they have a phenolic hydroxyl group, they have a property of binding with macromolecules such as proteins, Antioxidant, antimicrobial activity, and so on (Moon et al. 2004). The content of phenolic compounds in the fractions of Yeon - Kyung showed the highest value of 283.30 ± 0.029 ㎍ / ㎖ and 142.53 ± 0.02 ㎍ / ㎖ in EtOAc and BuOH fractions.

In addition, flavonoids, which are widely distributed in plants, are antibacterial, anticancer, antiviral, antiallergic, lipid-lowering, immune enhancing, capillary strengthening and anti-inflammatory activities. It is known to have an antioxidant effect that suppresses oxidation (Seo Eun et al. 2012). The content of flavonoid was highest in EtOAc and BuOH fractions as 94.60 ± 0.01 ㎍ / ㎖ and 43.90 ± 0.01 ㎍ / ㎖

The results are shown in Table 2 below.

Total Polyphenols and Total Flavonoid Content by Solvent Fraction of Salting Fraction Hexane EtOAc BuOH water Total polyphenol ([mu] g / ml) 15.47 ± 0.17 283.30 ± 0.29 142.53 + 0.02 101.90 ± 0.17 Total flavonoid ([mu] g / ml) 2.72 ± 0.16 94.60 ± 0.01 43.90 ± 0.01 37.43 + - 0.01

Experimental Example 3: Measurement of cytotoxicity of each fraction

(1) Cell culture and culture

Mouse RAW 264.7 macrophages were purchased from Korea Cell Line Bank (Seoul, Korea). Cell lines were cultured in DMEM (Dulbecco's Modified Eagle's medium: Dulbecco's modified Eagle's medium) at 10% Fetal Bovine Serum (FBS) and antibiotic antimycotic were added to the culture medium and cultured in a humidified incubator at 37 ° C and 5% CO ₂ .

Malassezia furfur (ATCC 14521), a causative organism of dandruff, was cultivated in YMB (Yeast Malt Broth, Difco, USA) medium containing 1% olive oil. The medium composition contained yeast extract, malt extract, peptone, and dextrose.

(2) Measurement of cell viability by fractionation

The viability of RAW 264.7 cell line was determined by Mosmann's method (Mosmann, 1983).

After reaching the logarithmic phase, the cells were adjusted to a concentration of 3 × 10 ⁴ cells / well in a 96-well plate. The cells were cultured for 24 hours and adhered and stabilized. After 24 hours of culture, the cells were diluted in the culture medium to a final concentration of 5 to 200 占 퐂 / ml, and the cells were treated for 24 hours. After completion of incubation, 10 μl of MTT solution (5 mg / ml in PBS) was added to each well, followed by reaction in a humidified incubator at 37 ° C and 5% CO ₂ for 4 hours to reduce MTT. The formazan crystals formed in each well were well dissolved in 150 μl of DMSO and absorbance was measured at 540 nm using a microplate reader (Molecular Devices, USA).

(3) Results

Cell viability was determined by MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide) assay for toxicity assays for RAW 264.7 cells. The MTT assay reacts with succinate-tetrazolium reductase, which is present in the respiratory chain of intracellular mitochondria and is active only in the surviving cell, to convert the yellow water-soluble substrate MTT to a bluish-purple water-insoluble MTT This is a test using the ability to reduce to formazan (Hongsik et al. 2010). As a result of treating the fractions of the almonds with concentration (5, 10, 20, 50, 100, 200 / / ml), toxicity was shown in a concentration dependent manner.

Experimental Example 4: Measurement of nitric oxide (NO) production inhibition by fraction

The amount of NO produced from RAW 264.7 cells was determined as the form of NO ₂ ^- present in the cell culture using a Griess reagent.

RAW 264.7 cells were adjusted to 1 × 10 ⁵ cells / well using DMEM medium, and then inoculated on a 24-well plate and cultured in a humidified incubator at 37 ° C. and 5% CO ₂ .

The cells were pretreated with 5, 10, 20, and 50 ㎍ / ㎖ concentrations of 1 μg / ml LPS and incubated for 18 hours. The cell culture supernatant was mixed with the same amount of Griess reagent and reacted on a 96-well plate for 10 minutes, and the absorbance was measured at 540 nm.

Concentrations of nitrite were compared using sodium nitrite (NaNo ₂ ) as a standard.

  LPS is present in the extracellular membrane of Gram-negative bacteria and is known to promote inflammation in RAW 264.7 cells by activating macrophage as an inflammatory substance (Yang, Sejin, and Taebu, 2011).

In order to confirm the anti - inflammatory activity of each aliquot, RAW 264.7 cells, a mouse - derived macrophage, were pretreated with a concentration of 5, 10, 20, 50 ㎍ / And the effect on inhibition of NO production was investigated. The results are shown in Fig.

The amount of NO produced after LPS (1 ㎍ / ㎖) was increased about 22 times as much as that of cell culture. The concentration of hexane fraction was determined to be 15.4 ± 0.33 μM / ㎖, 8.5 ± 0.24 μM / ㎖, 5.8 ± 0.06 μM / ㎖ and 2.7 ± 0.26 μM / ㎖, respectively, Acetate fractions were measured at 13.4 ± 0.17 μM / ㎖, 8.3 ± 0.79 μM / ㎖, 4.1 ± 0.30 μM / ㎖ and 1.3 ± 0.17 μM / ㎖ respectively and the butanol fractions were 21.3 ± 0.47 μM / ㎖ and 19.3 ± 0.23 μM / Ml, 16.7 ± 1.42 μM / ㎖ and 12.5 ± 0.62 μM / ㎖, respectively. The water fractions were 22.3 ± 1.30 μM / ㎖, 21.6 ± 1.20 μM / ㎖, 21.1 ± 0.86 μM / ㎖ and 20.8 ± 1.50 μM / ㎖, and NO production was inhibited in a concentration dependent manner.

Thus, the fractions of the ethanol extracts of the fractions showed significantly reduced NO production in the concentration range, and the ethyl acetate fraction showed the most inhibition of NO production.

Experimental Example 5: Measurement of antifungal activity by the fractions of almonds

The antimicrobial activity of the extract was measured using a paper disc method (Davidson 1989).

A single colony of pure isolates was used to inoculate 10 ml of the broth culture broth and incubated at the appropriate temperature for growth. The broth was used as a test strain for antimicrobial activity. The broth containing 15% agar The medium was sterilized and 15 ml of the medium was dispensed into petridish to coagulate the medium for the substrate. The optical density (OD) value of the test bacteria was set to 0.4 (10 ⁶ CFU / ml) at 650 nm , And 0.7% agar were added to the medium. A paper disk 8 mm in diameter was placed on the medium, and the fractions of the alveoli (125-1000 占 퐂 / ml) were absorbed, followed by incubation at 37 占 폚 and a clear zone around the disk was observed.

Dandruff is a skin disease caused by dandruff fungus. It causes dryness of the scalp as it becomes dry and itching accompanies it. Especially, it has a lot of oil in the hair and sebaceous secretion in the scalp is caused by the dust adsorbed on the hair well. Is not known.

Therefore, M. furfur , a sequential fraction of dandruff, The results of observing the formation of a clear zone by the paper disk method on the antifungal activity against the antifungal agent are shown in Table 3 below.

M. furfur The antifungal activity of the hexane fractions did not show clear zones at 125 ㎍ / ㎖ and clear zones of 10, 13 and 14 ㎜ from 250 ㎍ / ㎖, respectively. The EtOAc fraction showed a clear zone of 12 ㎜ even at 125 ㎍ / ㎖ and a high antifungal activity of 20 ㎜ at the concentration of 1000 ㎍ / ㎖. BuOH fractions and water fractions showed clear zones at a concentration of 500 μg / ml.

From the above results, it was found that the higher the concentration, the greater the antifungal activity. Especially, the highest activity was observed in ethyl acetate fraction.

Antifungal activity of extract fraction Clear zone on plate (Clear zone) (mm) sample 125 [mu] g / ml 250 [mu] g / ml 500 [mu] g / ml 1000 [mu] g / ml Hexane fraction ND ¹⁾  10  13   14 The EtOAc fraction  12  14  17   20 BuOH fraction  ND  ND  10   13 Water fraction  ND  ND  10   12

Formulation Example 1: Hair Essence

Hair essences containing an ethanolic extract of laxatives (lyophilized product) in Example 1 were prepared according to the composition components and composition ratios shown in Table 4 below.

Composition of hair essence ingredient Content (% by weight) Purified water 70.37 Orange number 13.00 glycerin 3.00 Polyglyceryl-10 myristate 5.00 The ethanol extract (lyophilized product) of Example 1 3.00 Betaine 2.00 Lavandin oil 1.00 Glycosyl trehalose 1.00 Dehydrozantanone 0.50 Hydrogenated starch hydrolyzate 0.50 Sodium benzoate 0.30 Benzyl alcohol 0.30 Citric acid 0.02 Dehydroacetic acid 0.01 Sum 100

Formulation Example 2: scalp tonic

The scalp tonic containing the lyophilized product of the ethyl acetate fraction of the elongated ethanol extract obtained in Example 2 was prepared according to the composition components and composition ratios shown in Table 5 below.

Composition of scalp tonic ingredient Content (% by weight) Purified water 79.68 Orange number 10.00 glycerin 3.00 Polyglyceryl-10 myristate 2.00 Betaine 1.00 The ethyl acetate fraction of Example 2 (lyophilisate) 1.00 Glycosyl trehalose 1.50 Hydrogenated starch hydrolyzate 1.00 Sodium benzoate 0.30 Benzyl alcohol 0.30 Lavandin oil 0.20 Citric acid 0.01 Dehydroacetic acid 0.01 Sum 100

Formulation Example 3: Shampoo

A shampoo containing the lyophilized product of the ethyl acetate fraction of the elongated ethanol extract obtained in Example 2 was prepared according to the compositional components and composition ratios shown in Table 6 below.

Composition of shampoo ingredient Content (% by weight) Purified water 46.99 Cocamidopropyl betaine 30.00 Number of green tea 5.00 Lauryl glucoside 10.00 Sodium lauryl glucose carboxylate 3.00 The ethyl acetate fraction of Example 2 (lyophilisate) 2.00 Dehydrozantanone 2.00 Sodium benzoate 0.30 Citric acid 0.30 Laban oil 0.20 Lemon peel oil 0.20 Hydrolyzed wheat protein 0.01 Sum 100

Experimental Example 6: Formulation stability

The stability of the formulations of Formulation Examples 1 to 3, which are the cosmetic compositions containing the extract of Yeast extract according to the present invention, was measured by the following methods.

A sample kept in a thermostat kept at 45 ° C in an opaque glass container for 12 weeks, a sample kept in a opaque glass container for 12 weeks in a fully shaded refrigerator maintained at 4 ° C, a sample kept at 45 ° C The precipitation of seaweed extracts was measured on the samples stored in the circulating chamber for 4 weeks.

As a result of the evaluation, precipitation was not observed at all, and it was evaluated to have a very good time stability.

While the present invention has been described in detail, the present invention is not limited thereto, and various changes and modifications may be made by those skilled in the art.

Claims (5)

A cosmetic composition for scalp comprising a lyophilized product of an ethanol extract of Forsythia viridissima . [Claim 7] The cosmetic composition for scalp according to claim 1, wherein the lyophilized product of the ethanol extract of the linkage is the lyophilized product of the ethyl acetate extract fraction of the ethanol extract. The cosmetic composition for scalp according to claim 1 or 2, wherein said lyophilized product is a concentrated and lyophilized product for filter filtrate of ethanol extract (1: 5 to 15 w / w) of tandem. The cosmetic composition for scalp according to claim 1, wherein the ethanol-extracted lyophilisate of the above-described crosslinking agent is contained in an amount of 0.01 to 50.0% by weight based on the total weight of the composition. The cosmetic composition for scalp according to claim 1, wherein the formulation of the cosmetic composition for scalp is a scalp serum, hair essence, shampoo, or scalp tonic.

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