KR101776202B1 - Natural preservative compositionpreservative composition and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a natural complex preservative composition and a manufacturing method thereof, and more specifically, to a natural complex preservative composition which contains a fermented product of a natural product and polyhydric alcohol, wherein the natural product is at least two selected from the group consisting of gallnut, Agrimonia eupatoria, dried clove buds, and goldthread. The natural complex preservative composition of the present invention is not toxic to human body, and has excellent antiseptic and antimicrobial effects, and thus can replace a synthetic preservative used in a cosmetic composition.

Description

[0001] The present invention relates to a natural preservative composition and a manufacturing method thereof,

The present invention relates to a natural combination preservative composition and a preparation method thereof, and more particularly, to a natural combination preservative composition comprising a fermentation product such as an obaginous, aphanaceae, clove, and chrysanthemum, a polyhydric alcohol, and a preparation method thereof.

Cosmetics contain a variety of nutrients and moisture, and microbes can grow well. In particular, glycerin, a carbon source of microorganisms, and sorbitol, amino acid derivatives and proteins, which are nitrogen sources, are mixed and are likely to be contaminated with bacteria and fungi.

 When cosmetics are contaminated by microorganisms, quality deterioration such as discoloration, detachment, viscosity and pH change appears. This is a phenomenon caused by the chemical action and biodegradation of respiration, metabolism, enzymatic action, etc., as the microorganism grows on the surface or contents. In the case of fungi, colonies are formed on the surface, the pH is changed by metabolism, and discoloration of the product occurs due to the pigment secreted by the contaminated microorganisms. During the metabolism of microorganisms, the viscosity may change due to the consumption of sugar, and the emulsion layer may be separated due to solidification of particles (Ryu, et al., 1992).

Preservatives are inevitably used for long-term protection of cosmetics that cause quality changes during use, and cosmetics are products that are repeatedly used on the skin for a long time, unlike medicines, so there is a greater need for preservatives. Therefore, ensuring the stability of products against microorganisms and proper preservative systems are an important part of cosmetic manufacturing technology.

 Currently, most cosmetics depend on synthetic preservatives, and its function is to impart safety and stability to the product by inhibiting the growth of microorganisms in the product (Cho, et al., 2008). Examples of such synthetic preservatives include parabens, quaternium-15, imidazolidinyl urea, chlorophenesin, phenoxyethanol, and MCI / MI series. While these synthetic preservatives have excellent antimicrobial activity and competitive price, they can cause skin irritation or human toxicity when used at high concentration for a long period of time. In addition, when synthetic preservatives accumulate in the body, they cause endocrine disruption, induce allergies, and cause skin irritation such as contact dermatitis and allergies.

Therefore, as the necessity of development of an alternative agent to overcome side effects of synthetic preservatives has been increased, researches have been conducted to develop natural preservatives containing natural antimicrobial substances as main components.

However, the natural antimicrobial materials conventionally developed have many preservative and antimicrobial effects than conventional synthetic preservatives.

On the other hand, the rhubarb is Rhiz javanica Linne or other insect house caused by stabbing Melaphis chinensis Bell (sopper aphid and Eriosomatidae) on the leaves of other plants (lacquer and Anacardiaceae). This medicine is odorless, tasteless and astringent. It is produced in various parts of the country. It contains 70 ~ 80% of gallotannin (tannin) and contains fat, resin and so on. It exhibits branching action, hemostatic action, antimicrobial action, and inhibition of branching by the convergent action of the main ingredient tannin. It is also used for diarrhea, colitis, dysentery, gastrointestinal bleeding, hemorrhage, nosebleeds, cold sweat, and shortness of breath in one room and also for traumatic bleeding, inflammation of the mucous membrane, ulcer and eczema (Chi, et al., 1998).

A. pilosa L. is a perennial herbaceous perennial belonging to the Rosaceae. It is also called as Yonga, Yongaeko, Hwanghaekcheon, Gyeongseoncho, , And has been used in folk remedies such as enteritis and pain relief (Y. Lee, et al., 2002). (H. Kwon, et al., 2005), a study on the anti-inflammatory activity using fractions (H. Correia, et al., 2006), antimicrobial activities of various bacteria A study of the effects (J. Bea, et al., 2005) has been made.

Clove is an evergreen small arboreous flower belonging to Myrtaceae. It is harvested and dried when the flower peaks become green to red. It has been widely used for various meat and vegetable dishes in European countries for many years. Is used for the purpose of enhancing flavor, suppressing bad flavor, insect repellent, controlling fungi, and the like. In particular, clove extracts have a wide range of physiological functions such as strong antioxidant, antimicrobial and antiviral activity, and thus they are widely regarded as natural functional materials (A. K. Singh, et al., 2009). To date, studies on cloves have reported on the antimicrobial effect of eugenol, the main flavor component of cloves, the effects on leukemia cell differentiation, and the inhibitory effect on allergic reactions, starting with thrombus-related studies (JI Lee, et al. (C. Wang, et al., 2010).

Coptidis Rhizoma is a rootstock that has almost eliminated the roots of Coptis japonica Makino (Japan), Coptis chinensis Franch (China) or other inbred plants (Ranunculaceae). Uneven columnar, 2 to 4 cm long, sometimes 10 cm long, 2 to 7 mm in diameter, slightly curved and sometimes cracked. The outer surface is yellowish brown with a rounded node, where the stem remains and the roots of many roots can be seen. It has a little odor, it is very tasty and it is yellowish in color with persistence. It is known that pharmacological effects are effective for vomiting due to diarrhea and stomach fever caused by accumulation of heat on the intestines and stomach due to the effects of dryness, soothing, anti-inflammation, and antibacterial activity.

 As disclosed in various papers, studies on a single substance have been actively carried out on dwarf, rhododendron, chrysanthemum, and chrysanthemum, but none of the prior art discloses a composition of a preservative mixed with Rhododendron, Rhododendron, Rhododendron and Rhodiola extract.

 Accordingly, the present invention has been accomplished by confirming that the effect of inhibiting microorganism growth is increased by fermenting a mixture of polyvalent alcohol with fermented cucurbits, Rhododendron japonica, clodshells, cloves and the like, which are known to have excellent efficacy.

KR 10-1513973 B1

Accordingly, an object of the present invention is to provide a natural combination preservative composition having excellent preservative effect and minimizing adverse effects on human body, and a method for producing the same.

To achieve the above object, the present invention provides a natural combination preservative composition comprising a fermentation product of a natural product and a polyhydric alcohol, wherein the natural product is at least one of cloves and rhizomes; gallnut; And Seon Hakcho; .

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The mixture is 100 parts by weight of the above-mentioned dwarf, 20-30 parts by weight of Seolka syrup, 20-30 parts by weight of clods and 80-120 parts by weight of Rhodophylla, and the mixture of Staphylococcus aureus KCCM 11335 ), Pseudomonas aeruginosa (KCCM 11802), Staphylococcus epidermidis (KCCM 35494), Escherichia coli (KCCM 40271) and Candida albicans (KCCM 11282).

The fermented product is characterized in that the fermented product is fermented into at least one of the genus Bacillus or the genus Saccharomyces.

The polyhydric alcohol is 1,3-butylene glycol.

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The natural composite preservative composition of the present invention is free of human toxicity and has an excellent antiseptic and antimicrobial effect, so that it can be substituted for a synthetic preservative used in a cosmetic composition.

Fig. 1 shows the results of evaluation of antibacterial activity against Staphylococcus aureus in Experimental Example 1 according to the present invention.
Fig. 2 shows the results of evaluation of antibacterial activity against P. aeruginosa in Experimental Example 1 according to the present invention.
3 shows the results of evaluation of antibacterial activity against Staphylococcus aureus in Experimental Example 1 according to the present invention.
Fig. 4 shows results of evaluation of antibacterial activity against Escherichia coli in Experimental Example 1 according to the present invention.
Fig. 5 shows results of evaluation of antibacterial activity against Candida albicans in Experimental Example 1 according to the present invention.

Hereinafter, the present invention will be described in detail.

In the present invention, a natural complex preservative means a mixture of two or more natural substances, which means that such a mixture works in combination to exhibit an excellent preservative effect. In the present invention, these natural products include at least two selected from the group consisting of a rhizomes, a rhododendron, a clod, and a rhododendron.

More specifically, the present invention is characterized in that it comprises a fermentation product of at least two natural products selected from the group consisting of dwarf, rhododendron, cinnabar and chrysanthemum, and a polyhydric alcohol. The reason why the natural products are used as two or more kinds in the present invention is to provide a superior preservative effect due to the synergistic action of the respective natural products, and when a single use of the rhizomes, But has no antimicrobial activity against certain bacteria. However, when two or more kinds thereof are mixed, antimicrobial activity against various bacteria is strongly shown.

In particular, the present invention relates to a process for producing a natural product, Seo, Hak - Cho; And clove, chrysanthemum, or both. In other words, it contains mandarin and rhododendron as essential, and selectively uses at least one of clods and rhododendrons. Staphylococcus aureus (KCCM11335), Psuedomonas aeruginosa (KCCM11802), Staphylococcus epidermidis (KCCM35494), Escherichia coli (KCCM40271) and Escherichia coli And Candida albicans (KCCM11282). When the antimicrobial activity against candida albicans is small, it can be used as a preservative for conventional cosmetic compositions Which has more potent antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli than 1, 2-hexanediol used, and even if it contains only three kinds of Aphaenopsis thaliana, Rhodophyceae and Rhododendron, Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus aureus, And Candida albicans, all of which have antimicrobial activity.

However, in order to have a strong antibacterial activity against all five microorganisms such as Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Candida albicans, it is most preferable to use a mixture of all four kinds of dicotyledonous sp.

At this time, the mixing ratio of the natural products is preferably 100 parts by weight of the dwarf, 20-30 parts by weight of sunflower seeds, 20-30 parts by weight of cloves and 80-120 parts by weight of chrysanthemum, considering antibacterial activity. In addition, even when three kinds of natural products are used, 100 parts by weight of daphnia magna; 20-30 parts by weight of sunflower seeds; And 20 to 30 parts by weight of clove or 80 to 120 parts by weight of chrysanthemum.

It is preferable that the natural product is fermented into one or more strains of genus Bacillus or Saccharomyces, because the use of such strains shows more excellent antibacterial activity. Specifically, Bacillus subtilis and Saccharomyces cerevisiae can be used.

In addition, as the polyhydric alcohol, 1,3-butylene glycol is preferably used because it is free from skin toxicity and is easy to extract the fermentation product.

In the present invention, the fermentation product of the natural product and the polyhydric alcohol, particularly 1,3-butylene glycol, are mixed at a weight ratio of 1: 0.8 to 2, particularly 1: 1 to 1.4, in terms of antimicrobial activity.

On the other hand, in order to obtain a better preservative effect, the present invention can further use coffee beans as a natural product.

That is, the unprocessed coffee beans are mixed with two or more kinds of natural products selected from the preceding two or more selected from the group consisting of dwarf, chrysanthemum, clove and chrysanthemum, and the mixture is fermented and mixed with a polyhydric alcohol. By weight.

Hereinafter, the production method thereof will be described. In describing the production method, the description of the composition has been omitted.

The method for preparing a natural combination preservative composition according to the present invention comprises the steps of: pulverizing at least two natural products selected from the group consisting of Oba, Zhaogejiao, Clove, and Huaxia; mixing the pulverized mixture with purified water; And then mixing the fermented fermented product with a polyhydric alcohol.

gallnut, Seonhak-cho , cloves , goldthread  ≪ RTI ID = 0.0 > 2 < / RTI >

First, a natural product containing at least two selected from the group consisting of a rhinoceros, a rhododendron, a clod, and a rhododendron is prepared. At this time, it is preferable to use all of the natural products in a dried state, but the present invention is not limited thereto.

Then, the prepared natural product is pulverized. At this time, since the pulverization is performed for smooth progress of fermentation, the particle size is not limited. And can be pulverized to an extent of about 100 to 200 mesh as an example.

In addition, the coffee beans can be optionally pulverized together with the above-mentioned natural products.

The pulverized mixture Purified Water  Mixing step.

Next, purified water is mixed with the pulverized product so that fermentation can be performed. The amount of the purified water to be added is not limited, but is a mixture for fermentation, so it is mixed in a conventionally known amount in the field to which this technology belongs.

And inoculating the mixed mixture with the bacteria to ferment.

Then, the pulverized product mixed with the purified water is inoculated with the microorganism and fermented. At this time, it is preferable to use at least one bacterium of the genus Bacillus or Saccharomyces as the bacterium. More specifically, it is preferable to use at least one bacterium selected from the group consisting of Bacillus subtilis and Saccharomyces cerevisiae One or both can be mixed and inoculated.

Here, the amount of inoculation may be about 10 ⁵ to 10 ⁸ cfu / g, and fermentation is carried out at 30 to 35 ° C. for 3 to 5 days after inoculation.

When the fermentation is completed, it is heat-treated, filtered with a filter paper, and then concentrated to complete the production of the fermented product. Here, the concentration method and the degree of concentration are not limited, and it is of course possible to dry in a powder state.

The fermented Fermented water  Mixing the polyhydric alcohol.

Finally, the fermented fermented product is mixed with a polyhydric alcohol, more preferably, 1,3-butylene glycol at a weight ratio of 1: 0.8 to 2, and extracted at 60 to 80 ° C for 20 to 30 hours to prepare a natural combination preservative .

Hereinafter, the present invention will be described in more detail with reference to specific examples.

≪ Example 1 >

Natural products were mixed in the proportions shown in Table 1 below in order to prepare natural preservative compositions containing at least two selected from the group consisting of Rhizobia, Rhodochrosophila, Cloves and Rhodiola. Bacillus subtilis and Saccharomyces cerevisiae were mixed at a concentration of 10 ⁵ cfu / g, respectively, in a ratio of 1: 1 by volume. Respectively. Next, it was incubated at 33 ° C for 4 days in an incubator.

After completion of the culture, the mixture was heat-treated at 60 ° C for 20 minutes, filtered through a filter paper, and then concentrated using a rotary vacuum evaporator to prepare a fermented product.

1,3-butylene glycol was mixed with the fermentation product at a ratio of 1: 1.2 by weight and extracted at 70 ° C for 24 hours to prepare a natural combination preservative composition.

In the above Examples 1-1 to 1-6, the above-mentioned habits, sunflower seeds, cloves and hwangryun were purchased and used in an in-herb dried state.

Mix ratio of Example 1. division A dwarf (g) Seonghak-cho (g) Clove (g) Chrysanthemum (g) Total amount (g) Example 1-1 160 40 40 160 400 Examples 1-2 200 - - 200 400 Example 1-3 - 60 60 280 400 Examples 1-4 160 - 80 160 400 Examples 1-5 160 80 - 160 400 Examples 1-6 280 60 60 - 400

≪ Comparative Example 1 &

And natural materials as shown in Table 2 were used.

The mixing ratio of Comparative Example 1. division A dwarf (g) Seonghak-cho (g) Clove (g) Chrysanthemum (g) Total amount (g) Comparative Example 1-1 400 - - - 400 Comparative Example 1-2 - 400 - - 400 Comparative Example 1-3 - - 400 - 400 Comparative Example 1-4 - - - 400 400

EXPERIMENTAL EXAMPLE 1 Measurement of Inhibition of Growth by Paper Disc Method [

The antibacterial effects of Examples 1-1 to 1-6 were measured using a paper disc method. The microorganisms used are Staphylococcus aureus (KCCM 11335), Psuedomonas aeruginosa (KCCM 11802), Staphylococcus epidermidis (KCCM 35494), Escherichia coli (KCCM 40271), Candida albicans (KCCM 11282) . The bacteria used in the test were cultured at 37 ° C for activation, and the fungi were cultivated at 25 ° C for activation, and diluted in a fresh liquid medium to a degree of one platinum. Then, each microorganism was plated on a proper medium, 20 μL of each sample was dispensed on a 6 mm paper disc, and incubated in an incubator to measure the antimicrobial activity against each strain by the diameter of the growth inhibitory circle formed around the paper disc.

At this time, 1, 2-hexanediol, distilled water (DW) and propylene glycol (PG) were used as a control group for each of the Examples and Comparative Examples.

The results are shown in Table 3 and Figs. 1-5.

Results of Experimental Example 1. division
Inhibition zone (mm) S.aureus P.aeruginosa S. epidermidis E. coli C.albicans Example 1-1 11 11 12 12 9 1,2-hexanediol 11 11 11 14 15 Examples 1-2 10 12 11 - 7 1,2-hexanediol 14 20 10 12 15 Example 1-3 8 7 8 - 11 1,2-hexanediol 12 18 10 12 18 Examples 1-4 13 13 13 10 - 1,2-hexanediol 13 13 10 11 16 Examples 1-5 11 12 13 9 8 1,2-hexanediol 12 15 13 10 14 Examples 1-6 12 16 15 12 - 1,2-hexanediol 12 12 9 14 15 Comparative Example 1-1 12 12 9 9 - 1,2-hexanediol 15 16 10 13 12 Comparative Example 1-2 8 - - 8 - 1,2-hexanediol 12 18 10 16 13 Comparative Example 1-3 - - 9 10 - 1,2-hexanediol 14 19 12 16 12 Comparative Example 1-4 10 - 9 10 7 1,2-hexanediol 12 13 11 17 12

As can be seen from Table 3 and FIGS. 1 to 5, the compositions of Examples 1-1 to 1-6 according to the present invention showed strong antibacterial effects as in 1,2-hexanediol. In particular, it was confirmed that the antibacterial effects of Examples 1-1 and 1-5 were excellent in all five microorganisms. In addition, Example 1-6 showed more excellent antibacterial effect than 1,2-hexanediol.

On the other hand, none of the five microorganisms of Comparative Examples 1-1 to 1-4 using only a single substance had antimicrobial effect, and the antimicrobial effect against each microorganism was also inferior to 1,2-hexanediol, It was judged that it was difficult to utilize it.

From these results, it was confirmed that the composition of the present invention can be used as a stable natural combination preservative composition instead of the conventionally used 1,2-hexanediol.

≪ Preparation Example 1: Preparation of cosmetic &

Formulation example  1-1. Flexible lotion

The composition of Example 1-1 was prepared according to a conventional method according to the composition shown in Table 4 below.

Composition of Formulation Example 1-1. Raw material name % (w / w) Purified water to 100 Dipropylene glycol 6.00 Denatured alcohol 3.00 glycerin 2.50 Betaine 0.50 PIGGY-60 Hydrogenated Castor Oil 0.35 Phenyl trimecicone 0.18 Acrylate / C10-30 alkyl acrylate crosspolymer 0.13 Bis-phage-18 methyl ethyl dimethylsilane 0.10 Tromethamine 0.09 Disodium iodide 0.13 Beauty 0.03 Example 1-1 2.00

Formulation example  1-2. Lotion

The composition of Example 1-1 was prepared in a conventional manner according to the composition shown in Table 5 below.

Composition of Formulation Example 1-2. Raw material name % (w / w) Purified water to 100 Propylene glycol 8.00 Hydrogenated polydecene 8.00 Caprylic / capric triglyceride 3.00 Olive oil 2.00 Butylene glycol 1.00 glycerin 1.00 Cetearyl alcohol 0.80 Dimethicone 0.80 Hydrogenated lecithin 0.80 Arachidyl alcohol 0.50 Sheer butter 0.50 Glyceryl stearate 0.50 FAGE -100 stearate 0.50 Stearic acid 0.50 Behenyl alcohol 0.60 Beauty 0.25 Arachidyl glucoside 0.20 Carbomer 0.17 Tocopheryl acetate 0010 Tromethamine 0010 Xanthan gum 0.06 Disodium iodide 0.03 Example 1-1 2.00

Formulation example  1-3. Nutrition essence

The composition of Example 1-1 was prepared according to the composition shown in Table 6 below by a conventional method.

Composition of Formulation Example 1-3. Raw material name % (w / w) Purified water to 100 Ammonium acryloyldimethyltauride / Vpicopolymer 0.180 glycerin 7.000 Dipropylene glycol 2.000 Butylene glycol 2.000 Disodium iodide 0.030 Hydrogenated lecithin 0.625 C12-16 alcohol 0.275 Palmitic acid 0.100 Cetearyl olivate 0.600 Sorbitan olivate 0.400 Panthenol 0.500 PP / PE-17/6 Copolymer 2.000 Carbomer 0.180 Acrylate / C10-30 alkyl acrylate crosspolymer 0.140 Octyldodecanol 2.000 Cetyl hexyl hexanoate 1.500 Dimethicone 0.500 Cyclopentasiloxane 1.400 Dimethicone 0.600 Tromethamine 0.240 Spices 0.200 Example 1-1 2.00

Formulation example  1-4. cream

The composition of Example 1-1 was prepared in a conventional manner according to the composition shown in Table 7 below.

Composition of Formulation Example 1-4. Raw material name % (w / w) Purified water to 100 glycerin 6.00 Dipropylene glycol 5.00 Caprylic / capric triglyceride 4.00 Sheer butter 3.00 Cetyl alcohol 2.30 Cyclopentasiloxane 2.25 Glyceryl stearate 2.00 Octyldodecanol 2.00 Dipentaerythritol hexahydroxystearate / hexa stearate / hexa rognate 1.50 C14-22 alcohol 1.20 Hydrogenated polyisobutene 1.00 Pertyltrimethicone 1.00 Sucrose polystearate 1.00 Cetearyl olivate 0.90 Cetearyl alcohol 0.80 Cyclohexasiloxane 0.80 Silica 0.60 Sorbitan olivate 0.60 Betaine 0.50 Bis-phage-18 methyl ethyl dimethylsilane 0.50 FAGE -100 stearate 0.50 Dimethicone 0.45 C12-20 alkyl glucoside 0.30 Dimethicone 0.30 Spices 0.25 Ammonium acryloyldimethyltaurate / Vpicopolymer 0.22 Disodium iodide 0.03 Example 1-1 2.00

<Experimental Example 2> Experiment to confirm human skin irritation>

In order to carry out the skin safety test, the degree of human skin irritation of the above Preparation Examples 1-1 to 1-4 was confirmed. To confirm human skin irritation, 25 human volunteers were subjected to a patch test for each cosmetic composition. However, those who are taking psoriasis, eczema, other skin lesions, pregnancy, breastfeeding or contraceptive, and antihistamines are excluded from this experiment.

10 ml of skins, lotions, essences and creams of Formulation Examples 1-1 to 1-4 were applied to the patches and then attached to the skin inside the arms for 24 hours. At this time, the test site was wiped with 70% ethanol before adhering the pellet, and then dried and attached.

 The photographs were taken 24 hours after the removal of the patches, 30 minutes after the patches were removed, the patches were attached, the patches were removed, the skin changes were observed, and the average values were observed in the International Contact Dermatology Research Group , ICDRG), and the results are shown in Table 9 below.

Evaluation standard. Criteria evaluation Average score Spoken No stimulation 0 Slight reaction and erythema Small stimulus One Erythema + hardening Light stimulus 2 Erythema + hardening + small cellulite Moderate irritation 3 Erythema + hardening + blister River stimulation 4

Results of Experimental Example 2. division Average skin irritation Formulation Example 1-1 0 Preparation Example 1-2 0.1 Formulation Example 1-3 0.1 Preparation examples 1-4 0.2

As shown in Table 9, all of the cosmetic compositions containing 2 wt% of the natural combination preservative composition of the present invention showed low irritation on the skin. Accordingly, the natural combination preservative composition according to the present invention was judged to be a safe cosmetic composition.

&Lt; Example 2 >

The same procedure as in Example 1-1 was carried out except that 160 g of dwarf gum, 35 g of acorns, 35 g of cloves, 150 g of chrysanthemum and 20 g of coffee bean were mixed and pulverized.

EXPERIMENTAL EXAMPLE 3 Measurement of Inhibition of Growth by Paper Disc Method [

Antimicrobial activity was measured in the same manner as in Experimental Example 1. The results are shown in Table 10 below.

Experimental Example 3 Results. division
Inhibition zone (mm) S.aureus P.aeruginosa S. epidermidis E. coli C.albicans Example 2 13 12 14 14 11 1,2-hexanediol 11 11 12 14 14

As shown in Table 10, it was confirmed that Example 2 of the present invention shows excellent antibacterial activity against five kinds of bacteria.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments.

Claims (6)

A fermentation product of a natural product and a polyhydric alcohol,
The natural product,
At least one of cloves and rhododendrons; gallnut; And Seon Hakcho;
Wherein the natural preservative is a natural preservative.
delete The method according to claim 1,
The natural products are dwarf, rhododendron, clove,
The mixture ratio is 100 parts by weight of the above-mentioned dwarf, 20 to 30 parts by weight of sunflower seeds, 20 to 30 parts by weight of clove and 80 to 120 parts by weight of plum,
The antimicrobial activity against Staphylococcus aureus (KCCM 11335), Psuedomonas aeruginosa (KCCM 11802), Staphylococcus epidermidis (KCCM 35494), Escherichia coli (KCCM 40271) and Candida albicans (KCCM 11282) &Lt; / RTI &gt;
The method of claim 3,
Wherein the fermentation product is fermented into at least one of the genus Bacillus and the genus Saccharomyces.
The method according to any one of claims 1, 3, and 4,
Wherein the polyhydric alcohol is 1,3-butylene glycols.
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