KR101266180B1 - Vegetable antibacterial composition and cosmetic composition for improving skin using the same - Google Patents

Abstract

The present invention relates to a vegetable antimicrobial composition and a cosmetic composition for improving skin using the same, and more particularly, to a cosmetic composition having an effect on skin improvement such as acne and the like, which is extracted from plants and has excellent antibacterial activity without safety and side effects. It is about.
The vegetable antimicrobial composition of the present invention contains a plant extract extracted from plant material including Eochocho, three hundred vine, golden, loquat, turmeric, Seokpo, Hamcho, and Ginseng as an active ingredient.

Description

Vegetable antibacterial composition and cosmetic composition for improving skin using the same}

The present invention relates to a vegetable antimicrobial composition and a cosmetic composition for improving skin using the same, and more particularly, to a cosmetic composition having an effect on skin improvement such as acne and the like, which is extracted from plants and has excellent antibacterial activity without safety and side effects. It is about.

Many products such as food, medicine, daily necessities, and cosmetics widely used in our daily life contain antiseptics, which are antimicrobial substances licensed for human use to prevent changes in internal properties and to preserve for a certain period of time.

As the material used as the preservative, there are some materials produced in nature, but almost all chemically synthesized artificial materials are used.

Most of the active substances used as preservatives are not commercialized due to color, deterioration of stability, narrow antimicrobial spectrum, formulation problems, and the like.Hinokitiol, a white powder extract, magnool, a magnolia extract, Only a portion of the grapefruit extract, such as DF-100 is being commercialized.

And even parabens preservatives, the safest synthetic preservatives and commonly used in household goods, cosmetics and medicines, are potential for skin allergies (Andrea Counti et al., Contact Dermatitis, 1997, 37; 35-36) and environmental hormones. (Edwin et al., Toxicology and applied pharmacology, 1998, 153; 12-19) and resistant bacteria.

Antibiotics are widely used to prevent and treat disease outbreaks in humans and livestock caused by microorganisms, but not to maintain products.However, various resistance strains appear due to misuse and abuse, so that high-level antibiotics are used in hospitals to treat infections. It is forming a vicious cycle of, and the susceptibility of causative bacteria to livestock is low, and antibiotic residue problem is emerging as a socially important issue in public health. Currently, two or more antibiotics may be used in combination to combat Methicillin Resistant Staphylococcus Aureus (MRSA) (Voss, A et al., Int J. Antimicrob. Agents, 1995, 5: 101-106) There are emerging strains that use vancomycin, but also to vancomycin, such as Vancomycin-resistant enterococci (Billot-klein D, Antimicrob. Agents. Chemother, 1992, 36: 1487-1490). have.

Recently, it has been reported to increase sensitivity by using flavonoids in natural products to increase the sensitivity of antibiotics (IAIN X, LIU et al., J. Pharm.Pharmacol, 2000, 52: 361-366), antibacterial peptide (Giacometti). A et al., J. Antimicrob. Chemother 2000 Nov; 46 (5): 807-811), have been studied as an alternative. However, antimicrobial peptides are limited in their antimicrobial properties and have problems in economic mass production methods (Lee JH et al. Protein Expr.Purif, 1998 Feb; 12 (1), 53-60).

The present invention has been created to improve the above problems, by developing an antimicrobial substance derived from nature that can replace the existing synthetic chemical antimicrobial material can be applied to various fields such as cosmetics, medicine, pesticides, food, household goods The object is to provide a vegetable antimicrobial composition.

Another object of the present invention is to provide a cosmetic composition that inhibits harmful bacteria of the skin and has an improvement effect such as acne, atopic salts and can be used safely without irritation to the skin.

The vegetable antimicrobial composition of the present invention for achieving the above object is characterized in that it contains a plant extract extracted from the plant material, including Eoseongcho, three hundred seconds, golden, loquat, turmeric, Seokchangpo, Hamcho, Gosam as an active ingredient.

The plant extract is characterized in that extracted by adding at least one extraction solvent selected from water, lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols or mixtures thereof to the plant material.

The plant extract is characterized in that it contains an extract extracted from the plant material further comprising a palm leaf as an active ingredient.

The plant extract is characterized by extracting by centrifuging the fermentation broth fermented by inoculating fermentation strain after inoculating the plant material to the medium.

And the cosmetic composition of the present invention for achieving the above object comprises an antimicrobial composition containing a plant extract extracted from plant materials, including Eoseongcho, three hundred seconds, golden, loquat, turmeric, Seokchangpo, Hamcho, Gosam, as an active ingredient, lotion , Lotion, cream, essence, pack, makeup base, foundation, body oil, body lotion, cleansing oil, cleansing foam, hair oil, hair shampoo and hair rinse, characterized in that formed in any one of the formulation selected from soap.

The present invention shows a wide range of antibacterial activity against a variety of bacteria, has an antioxidant effect, and is a safe substance for human use, which is useful for disinfection, antibacterial, and antiseptic purposes in various fields such as cosmetics, medicine, household goods, and pesticides. Is available.

In addition, due to the excellent antimicrobial effect is expected to be effective in improving the skin, such as acne, anti-inflammatory, atopy of the skin.

1 is a test report for the sterilization and antibacterial test,
Figure 2 is a photograph showing the results of the sterilization and antibacterial test.

Hereinafter, a vegetable antimicrobial composition according to a preferred embodiment of the present invention will be described in detail.

The vegetable antimicrobial composition of the present invention contains a plant extract extracted from plant material including Eochocho, three hundred vine, golden, loquat, turmeric, Seokpo, Hamcho, and Ginseng as an active ingredient.

The components and efficacy of the above plant materials will be described based on data known in the literature.

Houttuynia cordata (魚腥草) is of Houttuynia Cordata saururaceae (Houttuynia cordata Thunberg). Fish vinegar has pharmacological effects such as antibacterial, diuretic, hemostatic, analgesic, tissue regeneration, methyl-n-nonylketone, Laurylaldehyde, Capricacid, Cordarine , Active ingredients such as isoquercitrin, quercitrin, reynoutrin and hyperrin are used throughout the roots, stems, leaves and flower stems It is contained.

Three hundred seconds ( Saururus chinensis ) is a perennial herb belonging to over three hundred flowers, with 3 ~ 4 leaves white in July-August, and also called three-point white, full-white, white flower, white snow, white dugong, white bone, white cotton, etc. do. Recent studies on triticale include studies of the components of fatty acids and amino acids, studies of nitric oxide release mechanisms from peritoneal macrophages, studies of antimutagenic effects, and studies of anti-cancer and cytotoxic effects. have.

Golden ( Scutellaria baicalensis ) belongs to the labiatae, which uses roots and is known to be effective in antipyretic, bile secretion, gastric juice secretion, and atherosclerosis prevention. Major flavonoids contain 4-5% of baicalin, and other forms of wogonin, 7-methoxy baicalein, oroxylin-A, and school capflavone ) I, II, and sterols include beta-sitosterol, campesterol, and sugars such as sucrose and D-glucose, and other resins and essential oils. .

Loquat ( Eriobotrya) japonica ) is an evergreen tree belonging to the family Rosaceae, whose fruit, root, stem bark, and leaves are mainly used in Chinese medicine for medicinal use. Loquat means the fruit of loquat. Loquat also contains 90.26% of water, 67.30% of carbohydrates, 2.15% of fiber, 71.31% of reducing sugar, 3.24% of pentosan, and amino acids, polysaccharides, fatty sugars, cellulose, pectin, potassium, vitamin B1, vitamin C, tannin, sodium, calcium and In addition, it contains pigments such as cryptoxanthine and beta-carotene, and glucose, fructose, malic acid and the like. Loquat is effective for quenching thirst and lowering down the stomach, which is good for the stomach, lungs, and liver, as well as for the recovery of lung disease, hemorrhage, nasal blood, jogging, and vomiting.

Curcuma aromatica Salisb. Is a perennial herbaceous herbaceous plant that is a tuber of ginger, turmeric, turmeric, and buds. Turmeric contains d-camphor, sesquiterpenes (L-α curcumen and L-β-curcumen), sesquiterpene alcohols, curcumin, demethoxycurcumin, bismethoxycurcumin, turmeron, arturmeron, starch, fatty oil , Gums, yellow pigments, carbon and pelandenes. Curcumol, Kurdion, Tetramethylpyradine, Camphor, Borneol, Isobornene, Kurjeren, etc. It is known to contain 20 kinds of ingredients as main ingredients.

Seokchangpo (石); Acorus gramineus ) is the root of the plant and the plant, which has been traditionally used for the treatment of diseases such as anorexia due to gastroenteritis or fainting due to stroke, dementia, and mental disorder (Kang, Byung-su et al., Herbology) 2000)]. Studies on Seokchangpo were mainly conducted on the essential oil components [Fujita et al., Yakugaku Zasshi, 90 (11): 1367-1371 (1970); Fujita et al., Yakugaku Zasshi, 91 (1): 132-133 (1971)], and pharmacological action of central nervous system inhibition [Liao et al., J Ethnopharmacol, 61 (3): 185-193 (1998)]. , Bacterial antibiotic resistance inhibitory effect [Kim et al., Arch Pharm Res, 21 (6): 734-737 (1998)], neuronal cell death lowering effect [Cho et al., Life Sci, 68 (13): 1567 -1573 (2001)] and pharmacological studies on dementia treatment [US Patent Publication No. US6010702 (2000)] have already been published.

Hamchoe is an annual herb that grows on Gaepearl on the west coast of Korea. Its main ingredients are enzymes and minerals such as calcium, magnesium, potassium, iron, phosphorus, manganese, zinc, iodine, copper, nickel, chromium, cobalt, vanadium, sodium, chlorine, It contains a large amount of uronic acid and natural fiber, and contains general ingredients such as water, ash, crude protein, crude fat, carbohydrates, and vitamin B1, B2, vitamin C, vitamin E, niacin and alkaloids. In addition, amino acids (glutamic acid, aspartic acid, tyrosine, lysine, taurine, betaine, choline and proline are contained in a large amount.

Ginseng ( Sophora flavescens ) is a perennial plant belonging to the legumes. It is known to be toxic, bitter, and persistent, and it is known to be effective in diuresis, dysentery, fever, eczema, and antiarrhythmia in oriental medicine (Kee, CH, 1993. The pharmacology CRC Press, Inc., p. 63-66; Shin Mingyo, 1986. Clinical Herbal Sciences, Namsan Party, p.314-316). Several quinolizidine, alkaloid, flavonoid, saponin, etc. have been reported as components of the ginseng (Tang, W. and Eisenbrand, G., 1992. Chinese drugs of plant origin Springer-Verlag, Berlin, p.931-943; Ryu, SY, Lee, HS, Kim, YK and Kim, SH, 1997.Determination of isoprenyl and lavanduly positions of flavonoids from Sophora flavescens by NMR experment.Arch. Pharm. Res. 20: 491-495; Woo, ER, Kwak, JH, Kim, HJ and Park, HK, 1998.A new prenylated flavonol from the roots of Sophora flavescens.J. Nat. Prod. 61: 1552-1554).

The plant materials are mixed in a proportion to have excellent antimicrobial activity in the present invention. Plant materials may be mixed in the same weight ratio. The antimicrobial composition of the present invention may be prepared by mixing plant extracts extracted from each plant material in a predetermined ratio, or by mixing plant materials in a predetermined ratio and extracting them at once.

In addition to the above-described plant material may be further included to increase the antimicrobial activity. Firefly Leaf ( Agastache rugosa ) is a perennial plant with Lamiaceae, and is called by names such as millet, millet, zinnia or median grass. In the present invention, the mulberry leaves increase the antimicrobial activity of the antimicrobial composition and at the same time have an antioxidant activity to prevent oxidation.

Various extraction methods may be applied to obtain plant extracts from the aforementioned plant materials. One of the extraction methods can be obtained by adding an extraction solvent to the plant materials.

At least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixture thereof may be used as the extraction solvent. As the lower alcohol having 1 to 4 carbon atoms, methanol, ethanol and the like can be used. As the polyhydric alcohol, butylene glycol, propylene glycol, pentylene glycol and the like can be used. Mixtures of water and lower alcohols, mixtures of water and polyhydric alcohols, mixtures of lower alcohols and polyhydric alcohols, or mixtures of water and lower alcohols and polyhydric alcohols can be used as the mixture.

As an example of extraction, the mixture may be added to the plant material in an amount of 2 to 20 times by weight, and then mixed with hot water, cold needle or warm needle extract for 1 to 48 hours at 10 to 150 ° C. Further, a reflux cooling extraction method, an ultrasonic extraction method, or the like can be used. In addition to the above-described extraction method, an extract obtained through a conventional purification process is also included. For example, an active fraction obtained through various purification methods, such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies, etc., is also included in the extract. It goes without saying that the extract obtained by using the above-mentioned extraction solvent can be obtained in the form of powder through filtration, concentration under reduced pressure or freeze-drying, spray drying or the like.

Plant material is pulverized by the preferred extraction method, added to the medium, and then fermented broth is inoculated with fermentation broth to extract the fermentation by centrifugation. Each plant material is dried to less than 7% by weight of moisture, and then finely ground using a grinder. Grind is added to the medium. The medium may be prepared by mixing potato starch and dextrose in distilled water. For example, Potato Dextrose Broth (Difco, USA) may be used in which 1 g of distilled water is mixed with 4 g of potato starch and 20 g of dextrose. The addition amount of the pulverized product is 5 to 15 parts by weight based on 100 parts by weight of the medium. As a fermentation strain , it is used Aspergillus oryzae KCTC6292. After spawning 2 to 4 parts by weight based on 100 parts by weight of the culture medium spawns, it is fermented at 30 to 50 ℃ for 5 days to 8 days. In the present invention, the amyloglucosidase (AMG), Pectinex, and Viscozyme enzymes produced from the H. pylori during fermentation are involved in the fermentation process. These enzymes convert polysaccharides into various bioactive substances with antimicrobial and antioxidant activity.

After the fermentation process is completed, the fermentation broth is sterilized and then put in a grinder to finely grind and homogenized. Homogenized fermentation is centrifuged to take only the supernatant to obtain a plant extract. As such, by extracting the fermentation without applying heat, it is possible to prevent the destruction of the components and to use useful bioactive substances according to the fermentation.

The plant extract extracted by the above-described methods may be used as an antimicrobial composition per se or a known component may be used as an antimicrobial composition. Known ingredients can be various formulations for formulating antimicrobial compositions. In this case, the content of the plant extract in the whole antimicrobial composition is 10 to 99% by weight.

The antimicrobial composition may be used as a component of the cosmetic composition. The cosmetic composition of the present invention is preferably contained in 0.01 to 20% by weight of the plant extract relative to the total weight. If the plant extract is less than 0.01% by weight, the antimicrobial activity is insignificant, and if it exceeds 20% by weight, it is difficult to have an elevated effect compared to the content.

Cosmetic composition of the present invention by adding a variety of well-known ingredients and the like using methods known in the art, lotion, lotion, cream, essence, pack, makeup base, foundation, body oil, body lotion, cleansing oil, cleansing foam It may be prepared in any one of the formulations selected from hair oil, hair shampoo and hair rinse.

Specifically, when the formulation of the cosmetic composition of the present invention is a paste, cream or gel, pack, as known components, animal fibers, plant fibers, waxes, paraffins, starches, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, Silica, talc or zinc oxide and the like can be used.

When the formulation of the cosmetic composition of the present invention is a powder, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used. When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or sorbitan non-dispersion ester and the like can be used.

Water, liquid diluents such as ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester when the formulation of the cosmetic composition of the present invention is a suspension, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tragacanth and the like can be used.

Aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide ether sulfate when the formulation of the cosmetic composition of the present invention is a surfactant-containing detergent , Alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils or ethoxylated glycerol fatty acid esters and the like can be used.

In addition to the cosmetic composition of the present invention can be used as a conventional known ingredients such as stabilizers, solubilizers, vitamins, pigments and fragrances, can be prepared in any formulation commonly prepared in the art, Can be appropriately selected accordingly.

As an example of the formulation, the lotion includes 1.0 wt% of carbomer, 2.0 wt% of dimethicone, 5.0 wt% of jojoba oil, 0.5 wt% of provitamin E, 1.0 wt% of olive emulsion wax, 5.0 wt% of arbutin paste, and 1.2 wt% of T-mixture. %, 1.0% by weight of foam mix, 1.0% by weight of batine, 1.5% by weight of cetearyl alcohol, 4.0% by weight of glycerin, 5.0% by weight of flex oil, 5.0% by weight of arbutin paste, 5.0% by weight of plant extract, blending essence oil ( Trace amount), preservative (trace amount), and purified water (residual amount).

In addition, as an example of the formulation, the lotion may be 1.5% by weight of ethanol, 1.0% by weight of natural liquid, 3.0% by weight of betaine, 3.0% by weight of diethan panthenol, 1.0% by weight of sebum-A, 2.0% by weight of arbutin paste, 2.0% by weight of adenosine, It may be composed of 0.03% by weight of Eco gum, 4.0% by weight of glycerin, 1.0% by weight of collagen, 5.0% by weight of plant extract, preservative (trace), purified water (remaining amount).

In addition, as an example of the formulation, the foundation may be 2.0% by weight of stearic acid, 0.5% by weight of cetearyl alcohol, 1.0% by weight of glyceryl stearate, 0.5% by weight of polysorbate 60, 0.7% by weight of sorbitan sesquioleate, hydro 2.0% by weight of genelade seed oil, 5.0% by weight of sunflower oil, 6.0% by weight of squalane, 4.0% by weight of cetyloctanoate, 2.0% by weight of cyclomethicone, 5.0% by weight of butylene glycol, 3.0% by weight of glycerin Silicate 0.6%, xanthan gum 0.05%, titanium dioxide 10.0%, talc 3.0%, hydrated ferric oxide 1.3%, ferric oxide 0.3%, ferrus-ferric oxide 0.15%, preservative (trace) , Purified water (remaining amount), may be composed of 5% by weight of the plant extract.

In addition, as an example of the formulation, the hair shampoo is 0.5 wt% polyquaternium, 1.0 wt% natural liquid, 3.0 wt% cocamidopropyl betaine, 3.0 wt% betaine, 7.0 wt% CDA, LES 25.0 wt%, cocobetaine 5.0 wt%, Collagen 1.0 wt%, Diel Panthenol 3.0 wt%, Glycerin 4.0 wt%, Sebum-A 1.0 wt%, Adenosine 2.0 wt%, Plant extract 5.0 wt%, Blending essence oil (trace), Preservative (trace), It may be composed of purified water (residual amount).

In addition, as an example of the formulation soap is 90% by weight soap base, coconut oil and palm oil mixed in a weight ratio of 8: 2, plant extract 3.0% by weight, licorice extract 0.6% by weight, glycerin 4.0% by weight, tocopherol 1.2% by weight, aroma The composition may be 1.2% by weight of oil.

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

(Example 1)

Water extract was added 10 times by weight to a plant material mixed with Eoseongcho, three hundred seconds, golden, loquat, turmeric, Seokchangpo, Hamcho and Gosam in the same weight ratio, and heated at 100 ° C for 6 hours to filter the solids to prepare plant extracts. .

(Example 2)

The plant material of Example 1 was finely chopped, and 60% ethanol was added five times by weight, and the plant extract was prepared by cooling at room temperature for 24 hours.

Example 3

The plant material of Eoseongcho, three hundred seconds, golden, loquat, turmeric, Seokchangpo, Hamcho, and Ginseng dried at 5% by weight of water was mixed in the same weight ratio and finely pulverized using a grinder. After addition of 10 parts by weight of pulverized product with respect to 100 parts by weight of the medium receiving pre-sale from Korea Research Institute of Bioscience and Biotechnology (KRIBB) seed cultured hwanggukgyun (A. oryzae KCTC6292) 3 parts by weight was inoculated and fermented.

The medium used was Potato Dextrose Broth (Difco, USA), which mixed 4 g of potato starch and 20 g of dextrose in 1 L of distilled water, and fermented for 5 days by stirring at 120 rpm at 30 ° C. while injecting air at 30 vvm as a culture condition. . After fermentation, the supernatant obtained by milling the fermentation product finely using a grinder and centrifuging at 4000 rpm for 25 minutes was prepared as a plant extract.

Experimental Example 1: Antimicrobial Activity Experiment

Staphylococcus aureus as a test organism to measure the antimicrobial activity effective for the antimicrobial composition of the present invention (Staphylococcus The bactericidal and antimicrobial effects of the plant extract of Example 1 were tested using aureus ATCC 6538) and acne bacteria ( Protonibacterium acnes ATCC 6919). The test was conducted by the Korea Institute of Chemical Convergence Testing.

1. Test Methods for Sterilization

After culturing Staphylococcus aureus in a liquid medium, the cultured bacteria were diluted and adjusted to an initial bacterial count of 10 ⁵ to 10 ⁶ CFU / mL and used for the test. Test bacteria were added to 20mL of the sample, mixed, and then left at room temperature for 4 hours to determine the bactericidal reduction rate for the initial bacterial count by measuring the number of bacteria. Bacterial counts were measured as physiological saline, and the initial bacterial counts were expressed. However, in the initial dilution stage of all experiments, neutralization was carried out using D / E Neutralizing Broth (DIFCO). When bacteria were grown in the medium, the number of bacteria on the medium was multiplied by the dilution factor, (≪ 10) " by multiplying the dilution factor obtained in the neutralization step. The number of bacteria in all stages was measured using TSA. The viable cell count was calculated according to the following formula 1, and the bactericidal reduction rate was determined according to the following formula 2.

N = C x D (N: number of living cells, C: number of colonies, D: number of dilutions)

Equation 2: R (%) = [(A-B) / A] × 100 (R: bactericidal rate, A: initial bacterial count, B: bacterial count after a certain time)

2. Antibacterial test method

Acne bacteria were enriched in Fluid Thioglycollate Medium (DIFCO) medium and used for testing. Inoculate the acne bacteria solution evenly with a sterile swab on the prepared Brewer's Anaerobic Agar (DIFCO) plate medium, process 30μl of sample on the sterilized disc, place the disc in the center of the medium, and then Anaerobic Jar (DIFCO) at 35 ± 1 ℃. After measuring for 72 hours in anaerobic conditions using the measured diameter of the low zone (Inhibition Zone) formed.

3. Test results

The results of the bactericidal test for Staphylococcus aureus and the antimicrobial test results for acne bacteria are shown in Tables 1 and 2, FIGS. 1 and 2, respectively.

division Early After 4 hours Sample (Example 1) 6.1 × 10 ⁵ ≪ 10 (99.9% or more)

As a result of Table 1, the initial inoculum was reduced to [<10 (less than 10) CFU / mL] after 4 hours treatment at 6.1 × 10 ⁵ CFU / mL. It is 99.9% or more of the bactericidal power, it can confirm the excellent bactericidal power for the antimicrobial composition of the present invention.

division Overall diameter Diameter of sample and disc Width of lowlands Sample (Example 1) 12.0 8.0 2.0

As a result of measuring the size of the low zone, as shown in Table 2, a low zone of 2.0 mm was formed for acne bacteria. This can confirm the excellent antimicrobial activity against the antimicrobial composition of the present invention.

Experimental Example 2: Skin Test Experiment

Skin tests were conducted to determine the extent of skin irritation. For skin testing, a group of 20 men and women (mean age 23.5 ± 1.5 years) had a special patch (IQ chamber, Chemotechnique, Sweden) containing a sample attached to their back and removed 24 hours later and read out 30 minutes later. 24h single epicutaneous patch test was performed. The degree of response of the skin was determined as 1-4 points according to the ICDRG (International contact dermatitis research group) evaluation standard table for erythema, edema, crust and shallow ulcer, and recorded as a total average value. As a sample, 5 wt% of the plant extracts of Examples 1 to 3 and 95 wt% of purified water were mixed to prepare first, second, and third test samples.

Probability of skin irritation is a mean score of 4.5 or more in 4 or more patients (20% of the total subjects). (ICDRG score index 0-1.5: no skin irritation, 1.5-2.5: mild erythema with no skin irritation, 2.5-4.5: mild erythema and edema, further confirmation test required> 4.5: possible skin irritation)

division Skin irritation degree (ICDRG average score index) 1st sample <1.5 2nd test sample <1.5 Third test sample <1.5

From the results of Table 3, it was confirmed that all three samples did not show erythema or edema reactions that could cause significant skin irritation in the test subjects. Therefore, when the cosmetic composition of the present invention is applied to the skin can be used safely without irritation. From this, the cosmetic composition of the present invention is expected to be effective in improving skin such as acne with excellent antibacterial effect.

In the above, the present invention has been described with reference to one embodiment, which is merely exemplary, and it will be understood by those skilled in the art that various modifications and equivalent embodiments are possible.

Accordingly, the true scope of protection of the present invention should be determined only by the appended claims.

Claims (5)

delete delete delete delete It contains 0.01 to 20% by weight of plant extracts extracted from plant material containing Eoseongcho, three hundred seconds, golden, loquat, turmeric, Seokchangpo, Hamcho and Gosam in the same weight ratio,
Lotion, lotion, cream, essence, pack, makeup base, foundation, body oil, body lotion, cleansing oil, cleansing foam, hair oil, hair shampoo and hair rinse, soap, any one of the formulation selected from,
The plant extract is 10 parts by weight of the pulverized product of the plant material dried at 5% by weight of water with respect to 100 parts by weight of medium containing 1 g of distilled water and 4 g of potato starch and 20 g of dextrose, and 3 weight of H. erythrocytes ( Aspergillus oryzae ). After adding the part, while stirring the air fermentation broth was stirred for 30 days at 120 ℃ at 30 ℃ while injecting air at 30vvm and then a supernatant obtained by centrifugation for 25 minutes at 4000rpm, the cosmetic composition for skin improvement.

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