KR100986249B1 - Natural antimicrobial and antiseptic substance made from Dryopteris crassirhizoma root extract complex - Google Patents

Abstract

The present invention is mixed with an extract containing 0.2 to 2.0% by weight of the extract of one or more extracts selected from the group consisting of 0.2 to 2.0% by weight lotus leaf extract, 0.1 to 2.0% by weight red wine extract, 0.2 to 2.0% by weight cinnamon extract It is a cosmetic composition having a composite natural antibacterial and antiseptic activity.

The cosmetic composition of the present invention is a mixture of extracts in an appropriate ratio using domestic medicinal plants as a raw material and can be effectively used as a natural preservative by showing excellent antimicrobial activity due to the cooperative action between plant extracts.

Natural antibacterial, natural preservative, spectators, lotus leaf, red wine, cinnamon, cosmetic composition

Description

Spectrum extract and natural antibacterial and antiseptic composition using the same. {Natural antimicrobial and antiseptic substance made from Dryopteris crassirhizoma root extract complex}

The present invention relates to a method for producing a natural complex extract using a composite extract, lotus leaf extract, red pepper extract, cinnamon extract and a natural antibacterial, preservative composition using the same.

In the present invention, in order to develop a preservative from a natural substance that can compensate for the irritation that is a disadvantage of the parabens, which have been widely used as a cosmetic preservative, it contains a spectral extract and a complex ratio appropriately using a red yang extract, a duct extract, a cinnamon extract By mixing with the mutual cooperation between the extracts to describe a composition having a better antibacterial, antiseptic properties.

The deterioration factors of cosmetics can be classified into factors caused by physical and chemical changes and factors caused by microbial contamination. Among them, the problem of microbial contamination is increasing due to the deterioration of the product value due to the deterioration of the product itself as well as the hygienic aspects of the skin use area.

Since cosmetics use many nutrients derived from natural products and raw materials that are easily decomposed by microorganisms, cosmetics are easily contaminated by microorganisms such as mold, yeast, and bacteria during distribution or consumer use. To prevent this, preservatives should be added and the maximum amount of antimicrobial activity should be shown. Therefore, the formulation, pH, emulsification form, mixed use of preservatives and its stability should be taken into consideration, as well as antimicrobial activity and skin irritation of all ingredients in the product in advance. It is very important to choose an appropriate preservation system.

Microorganisms are characterized by their ability to grow in the presence of moisture under temperature, pH or any other special environment. Therefore, preservatives are always needed in most cosmetic products having high water activity, Aw ≥ 0.90. When looking at the components of cosmetics, water, nitrogen sources, minerals, vitamins, oxygen, pH, etc., which are generally necessary for the growth of microorganisms, are at a suitable temperature in the distribution process of the product, and thus almost all of them have conditions for easy growth of microorganisms. Therefore, when cosmetics are contaminated from microorganisms, characteristic phenomena appear depending on the components of the contaminated microorganisms. For example, carbohydrates decompose and produce acids or gases and bubbles on their surfaces. Fatty acids and ketone residues are produced by rancids, and protein and related degradation products are metabolized to cause off-flavor. Therefore, the cosmetics are always easy to be contaminated by microorganisms, and use preservatives to solve the preservation problems. In particular, products containing no alcohol and sunscreen products with a lot of oil, the faster the growth of microorganisms. As a result, the preservative must be safe, stable, inexpensive, easily applicable, and have good compatibility with the components in the product and the container.

Environmental pollution due to rapid industrialization and life in complex metropolitan cities make modern people admire more naturalistic life. Directional phenomenon is prominent. In the cosmetics industry, naturalist cosmetics, which exclude chemicals and use organic ingredients, claim to be pure vegetable ingredients, are gaining popularity. With the development of cosmetics using various natural materials, the era of the heyday of natural cosmetic herbal cosmetics is coming. According to this consumption trend, it is advertised as 'unnatural preservatives of natural materials', 'no artificial parabens or synthetic chemical raw materials', and '100% natural raw materials'. However, tests at several of these companies showed that preservatives such as methylparaben, phenoxyethanol, sorbic acid, and benzoic acid were detected, as well as bacteria and fungi.

Due to these problems, studies on natural preservatives with excellent safety and economical efficiency of products remain to be solved. As a result, natural antimicrobial substances produced by medicinal plants, marine organisms, insects and microorganisms have been reported. However, most of the reported natural antimicrobial substances are not commercialized due to color, off-flavor, deterioration of stability, narrow antimicrobial spectrum, and formulation problems.They are Hinokitiol, a magnolia extract, Magnolol, Magnolol, and grapefruit. Only a fraction of the extract, such as DF-100 is commercialized.

An object of the present invention is to provide a method for producing a natural complex extract using a composite extract, lotus leaf extract, red leaf extract, cinnamon extract and a natural antibacterial, preservative composition using the same.

In order to achieve the above object, the present invention relates to a cosmetic composition using an extract having antibacterial and antiseptic activity, the extract containing 0.2 to 2.0% by weight of the spectral extract, the lotus leaf extract of 0.2 to 2.0% by weight, the red extract 0.1 to 2.0% %, Cinnamon extract is a cosmetic composition having a complex natural antibacterial and antiseptic activity by mixing at least one extract selected from the group consisting of 0.2 to 2.0% by weight.

The cosmetic composition contains a spectral extract and lotus leaf extract, red yang extract, cinnamon extract in an appropriate ratio, showing excellent antimicrobial activity according to the interaction between the extract can be effectively used as a natural preservative.

The spectators of the present invention are root stems of a perennial herbaceous plant ( Dryopteris crassirhizoma Nakai), which is a bitter , cool, roots grow crooked , thick lumps, hard, with many traces of petioles and beard roots. There is a big dark brown large vinyl. It grows in shaded wet land in the forest of relatively deep mountainous regions of almost all of Korea. It is distributed in China, Japan, and Russia worldwide. In autumn or early spring, cut the rhizome, trim the roots and scaly leaves, cut them horizontally or vertically with the undersides of the leaves and dry them in the sun. About 7% of the phylysin in the rhizome, flavantannin-based tannins (7-10%), oil (5-6%), flavonoids, etc. are known. The parasitic component is a phloroglucine derivative in which two to three molecules are combined with phylic acid and phylisine butanone via methylene. Among these derivatives, aspirin and albapidine have the highest antiparasitic effect. The pharmacological effects of the roots are various physiological activities such as antiparasitic, antiviral, antibacterial, uterine, hemorrhage, nosebleeds, hemorrhoids, hemostasis, hemorrhoids, and heavy loads. It is not good for patients with gastrointestinal ulcers, but in general, it has not been absorbed from the stomach and has been used as a herbal medicine for a long time.

Lotus leaf of the present invention is a perennial aquatic plant of water lilies ( Nelumbo nucifera Gaertn.) Leaf area, with mild, sweet and mediocre taste. As well as a symbol of longevity and health, the leaves of the lotus, which show honor, eternal life, and luck, are native to tropical regions such as India and Egypt, and are widely grown in Korea, Japan, China, and Siberia. The leaves of lotus, Loe Merin, Nuciferin, Nolanciferin, etc., and pharmacological action to stop diarrhea in the summer and to relieve headaches and dizziness. It has also been used to stop bleeding, nosebleeds, fecal bleeding, uterine bleeding, and to treat dizziness caused by bleeding after giving birth.

Red sheep of the present invention is a deciduous large tall tree of the birch ( Alnus japonica Steud.) The young eggplant and the bark are sweet, cool, and plain. It grows in the foothills, mountainsides, and valleys below about 1,000m above sea level in northern, central, southern part of Korea. It is distributed worldwide in China (Northeast), Russia (Far East) and Japan. Peel and dry in the sun in the spring, which contains tannins (5-10%), the trikerpenoids taraxerol, and betulinic acid. The pharmacological effect was clearing and strengthening, so it was used for nasal bleeding, diarrhea and traumatic bleeding.

Cinnamon of the present invention is an evergreen big-key tree cassia ( Cinnamomum The dried bark of cassia Preni .), temper is hot and warm. In Chinese medicine, it is an important herbal medicine that is widely used for the treatment of headache, fever, nervous system, pain, and cold. The main component of cinnamon is an aromatic compound, which is used as cinnamic aldehyde drink, chewing gum, toothpaste, fragrance component of cosmetics, and bad breath remover. Cinnamon's physiological activity has been reported to include antimicrobial effect, antimutagenicity, anticancer effect, anti-ulcer effect, egg's immune antibody enhancement effect, anti-allergic effect and complement system activation.

The spectral extract of the present invention may be divided into spectral parts of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diethyl ether. It is extracted with one or more solvents selected from the group consisting of.

Extraction method is prepared by immersing the fine audience at a stable temperature in a solvent or by heating under reflux extraction in an extractor equipped with a cooling capacitor, concentrated by filtration and concentrated under reduced pressure and freeze-dried the concentrated concentrate. In addition, after performing the extraction process may include a low temperature aging or high temperature aging process.

Lotus leaf, red sheep, cinnamon extract of the present invention is cut into lotus leaf, red sheep, cinnamon, respectively, water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonite It is extracted with one or more solvents selected from the group consisting of reel, petroleum ether and diethyl ether.

Extraction method is prepared by immersing the fine lotus leaf, red lamb, cinnamon each in a stable temperature in a solvent or extracted by heating to reflux in an extractor equipped with a cooling capacitor, and then filtered and concentrated under reduced pressure and freeze-dried the concentrated concentrate. In addition, after performing the extraction process may include a low temperature aging or high temperature aging process.

In addition, the cosmetic composition according to the present invention may contain other ingredients that can give a synergistic effect to the main effect in addition to the above extract without impairing the main effect of the present invention. The weight, lotus leaf, red leaf, cinnamon extract may be present in the composition in an amount of 0.1 to 10% by weight based on the total lyophilized weight.

If the plant extract is present in the form of a solution or powder in the form of nanocapsules, microcapsules, nanosomes, etc. containing the extract, those skilled in the art can adjust the amount of the solution or powder in the composition according to the invention within the concentration range There will be.

It is provided to cosmetics, foods, pharmaceuticals, pesticides, household goods and the like processed with the antimicrobial composition according to the present invention having a broad antimicrobial spectrum as described above.

The antimicrobial composition of the present invention is not particularly limited in its formulation as an external preparation for skin, and it is a basic cosmetic and foundation such as softener, milk lotion, nourishing cream, massage cream, essence, cleansing foam, cleansing water, pack or body oil, lipstick It may be prepared in the form of a color cosmetic such as mascara or makeup base. When the cosmetic of the present invention is used as a detergent, a face wash and a bath roll can be prepared.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples, which are only presented to aid the understanding of the present invention, but the present invention is not limited thereto.

As described above, the antimicrobial and antiseptic composition according to the present invention is mixed with spectral extracts and lotus leaf extracts, red leaf extracts, cinnamon extracts at an appropriate ratio, showing excellent antimicrobial activity due to the cooperative interaction between plant extracts, It is wide and is excellent in preservative ability for cosmetics.

Although the present invention has been described in terms of the preferred embodiments, various modifications may be made by those skilled in the art, and the modifications should be protected according to the claims. The antimicrobial and antiseptic composition according to the present invention can be usefully used for sterilization, antibacterial and antiseptic purpose in various fields such as medicine, household goods, pesticides as well as cosmetics.

Example 1 Preparation of Spectrum Extracts

The dried weight 200g of the crushed tube was heated and refluxed for 6 hours with 70% (V / V) ethanol aqueous solution, and then cooled and filtered through a filter paper having a permeation size of 8 μm. The filtered extract was concentrated under reduced pressure at 60 ° C. or lower and completely dried using a reduced pressure dryer to obtain 15.6 g of the extract in the tube.

Example 2 Preparation of Lotus Leaf Extract

The dried lotus leaf having a dry weight of 200 g was heated to reflux for 6 hours with an aqueous 70% (V / V) ethanol solution, and then cooled and filtered. The filtered extract was concentrated under reduced pressure at 60 ° C. or lower, and dried completely using a reduced pressure dryer to obtain 17.5 g of lotus leaf extract.

< Example  3> Red Extract  Produce

The dried red ginseng with a dry weight of 200 g was heated to reflux for 6 hours with an aqueous 70% (V / V) ethanol solution, cooled, and then filtered through a filter paper having a permeation size of 8 μm. The filtered extract was concentrated under reduced pressure at 60 ° C. or lower and completely dried using a reduced pressure dryer to obtain 3.3 g of red yang extract.

< Example  4> Preparation of Cinnamon Extract

Crushed cinnamon with a dry weight of 200 g was heated to reflux for 6 hours with an aqueous 70% (V / V) ethanol solution and then cooled and filtered through a filter paper having a permeation size of 8 μm. The filtered extract was concentrated under reduced pressure at 60 ° C. or lower and completely dried using a vacuum dryer to obtain 12.3 g of cinnamon extract.

< Experimental Example  1> Antimicrobial Activity Assay

In order to investigate the antimicrobial activity spectrum of the extracts was used the plate diffusion method (Kirby- Bauer method). The strains used were Gram-positive bacteria (Bacillus subtilis ATCC 6633), Staphylococcus aureus ATCC 6538, Gram-negative bacteria (Escherichia coli ATCC 9637), Pseudomonas aeruginosa ATCC 9027, Yeast (Candy) Candida albicans ATCC 10231) was used by the Korea Institute of Bioscience and Biotechnology.

Bacteria were used as Lesine broth, inoculated with the bacteria, and inoculated and cultured at 37 ° C. incubator for 24 hours. Yeast was used YM Broth (YM Broth) and inoculated with the culture medium was used for two days pre-culture in a 25 ℃ shaking incubator.

For more specific antimicrobial test, inoculate bacteria in Lesinen broth, pre-culture at 37 ℃ for 24 hours, and inoculate bacteria in YM broth in yeast. After 2 days pre-incubation at 25 ° C, the cells were diluted in sterile saline solution and the bacteria were spread on each agar plate medium at a germ concentration of about 1 × 10 ⁶ CFU, and the yeast was agar plated at a germ concentration of about 1 × 10 ⁵ CFU. Smear on the medium. Place a sterile 8mm paper disc on which 50 μl of each extract was absorbed at a concentration of 1%, and gently press the extract. Then, bacteria were incubated at 37 ° C. for 24 hours and yeast were incubated at 25 ° C. for 2 days, and then compared by measuring the size of the clear zone around the disc (mm).

division Antibacterial Spectrum B. subtilis S. aureus E. coli P. aeruginosa C. albicans Spectator extract 10.5mm 11 mm 10 mm 10 mm 10 mm Lotus Leaf Extract 9 mm 10 mm 8 mm 8 mm 9 mm Red Extract 8 mm 15 mm 10.5mm 9.5 mm 8 mm Cinnamon Extract 16 mm 12 mm 13 mm 9 mm 11 mm

As shown in Table 1, the spectral extract showed excellent antimicrobial activity against Gram-positive bacteria, Bacillus subtilis, Staphylococcus aureus, and also against Gram-negative bacteria Escherichia coli, Pseudomonas aeruginosa and Candida yeast. Lotus leaf extract showed good antimicrobial activity against Bacillus subtilis, Staphylococcus aureus and Candida yeast, but no range of antimicrobial activity was observed for Escherichia coli and Pseudomonas aeruginosa. Red extracts showed excellent antimicrobial activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, and cinnamon extract showed excellent antibacterial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida yeast. .

< Experimental Example  2> antibacterial test

In order to evaluate the antimicrobial activity of the extracts by determining the inhibitory power by concentration to determine the minimum inhibitory concentration. The strains used were strains used for the antimicrobial activity assay and the preparation procedure for each strain was the same as the above method.

More specifically, 2 ml of each extract, diluted to an appropriate concentration in 5% Dimethylsulfoxide (DMSO) saline solution for each test to be tested, was added to a sterile petri dish, and 2 ml of 5% Dimethylsulfoxide saline solution was used as a control. After sterilization in each Petri dish, Lesinen agar medium (Letheen agar) and YM agar medium (YM agar), which were maintained at 50 ° C., were added to the sample solution in a ratio of 9: 1 by 18 ml, mixed well, and left to solidify.

Once the medium has solidified, each of the precultivated test bacteria is inoculated into each petri dish at a final concentration of approximately 1 to 5 × 10 ⁶ CFU for bacteria and 1 to 5 × 10 ⁵ CFU for yeast. Inoculate each Petri dish at a bacterial concentration. Each petri dish was cultured at 37 ° C. and yeast at 25 ° C. for 1 to 5 days, and then visually observed colony formation. The minimum inhibitory concentration (MIC) is the minimum concentration of the antimicrobial agent on the plate that inhibits the growth of bacteria.

division Inhibitory concentration (% by weight) B. subtilis S. aureus E. coli P. aeruginosa C. albicans Spectator extract 0.25 0.25 1.0 0.5 1.0 Lotus Leaf Extract 0.25 0.25 1.0 1.0 0.5 Red Extract 0.25 0.125 1.0 0.5 1.0 Cinnamon Extract 0.25 0.25 0.5 1.0 0.5

As shown in Table 2, the spectral extract showed excellent antimicrobial activity even at concentrations of 0.5% by weight or less against Gram-positive bacteria such as Bacillus subtilis and Staphylococcus aureus, but also showed relatively weak antimicrobial activity against Pseudomonas aeruginosa. It also showed very weak antimicrobial activity against yeast and Candida yeast.

Lotus leaf extract showed excellent antimicrobial activity against subtilis of Staphylococcus aureus and Staphylococcus aureus at a concentration of 0.25% by weight or less, but showed relatively weak antimicrobial activity against Candida yeast and very weak antibacterial activity against Escherichia coli and Pseudomonas aeruginosa. It was. In the case of red yang extract, it showed excellent antibacterial activity at concentrations of 0.25% by weight or less against Staphylococcus aureus, but relatively weak antibacterial activity against Bacillus subtilis and Pseudomonas aeruginosa, and also very weak against E. coli and Candida yeast. It was. Cinnamon extract showed good antimicrobial activity at concentrations below 0.5% by weight against Bacillus subtilis and Staphylococcus aureus, but relatively weak against E. coli and Candida yeast and very weak against Pseudomonas aeruginosa. Appeared.

< Experimental Example  3> emulsion  Base manufacturer

An emulsion base containing the cosmetic containing the extract and a conventional chemical preservative was prepared. The cosmetics used in the experiments are in the form of emulsified cosmetic liquids, the composition of which is shown in the table. First, add the plant extracts or chemical preservatives listed in the table by weight, and then a) heat the phase to keep it at 90 ° C, and add purified water (30%) to 90 ° C, and slowly add it in the water phase. Emulsify uniformly. After cooling to room temperature, the remaining b) purified water was added and stirred uniformly to prepare a cosmetic liquid.

division ingredient weight% end glycerin 3.00 Disodium ID 0.02 Polyglyceryl-3-methylglucose distearate 1.50 Cetearyl Alcohol 0.50 Caprylic / capric triglyceride 7.00 Polyacrylamide & C13-14 Isoparaffin & Laures-7 0.60 Antibacterial natural products or preservatives Quantity I Purified water to 100

< Example  5 to 10> containing antimicrobial active extract emulsion  Manufacture of base

According to the composition of Table 4 by the method of Experimental Example 3 was prepared an emulsion base containing the added amount of the extract of Examples 5 to 10.

ingredient Example 5 Example 6 Example 7 Example 8 Example 9 Example 10 Spectator extract One% - - - 0.5% 0.5% Red Extract - One% - - - 0.5% Lotus Leaf Extract - - One% - - - Cinnamon Extract - - - One% 0.5% -

< Example  11 to 14> containing antimicrobial active extracts emulsion  Manufacture of base

According to the composition of Table 5 by the method of Experimental Example 3 was prepared an emulsion base containing the added amount of the extract of Examples 11-14.

< Comparative example  1 to 2> emulsion  Manufacture of base

According to the composition of Table 5, the emulsion base of Comparative Example 1 containing a chemical preservative and Comparative Example 2 containing no chemical preservative and the extract was prepared by the method of Experimental Example 3.

ingredient Example 11 Example 12 Example 13 Example 14 Comparative Example 1 Comparative Example 2 Spectator extract 0.5% 0.5% 0.5% 0.25% - - Red Extract 0.25% 0.25% - 0.25% - - Lotus Leaf Extract - 0.25% 0.25% 0.25% - - Cinnamon Extract 0.25% - 0.25% 0.25% - - Methyl Paraben - - - - 0.4% - Profile paraben - - - - 0.2% - Phenoxyethanol - - - - 0.4% - Purified water - - - - - One%

< Experimental Example  4> in formulation Antiseptic  Experiment

In order to determine the antiseptic force in the formulation containing the extract, an antiseptic activity test of the emulsion base prepared in Examples 5 to 14, Comparative Example 1 and Comparative Example 2 was performed. Preservative effectiveness testing is important for product safety and consumer acceptance as it seeks to find the minimum concentration of preservatives needed to safely preserve the product without causing skin irritation to the consumer. To measure the preservation of cosmetics, USP and CFA are widely used. This test method used the microbial guidelines of the Cosmetic, Toiletry, and Fragrance Association (CTFA).

The strains used were Gram-positive bacteria (Bacillus subtilis ATCC 6633), Staphylococcus aureus ATCC 6538, Gram-negative bacteria (Escherichia coli ATCC 9637), Pseudomonas aeruginosa ATCC 9027, Yeast (Candy) Candida albicans ATCC 10231) was used. Letheen agar was inoculated with four kinds of bacteria, and YM agar was inoculated with yeast. Bacteria were incubated at 37 ° C. and yeast at 25 ° C. for 24 to 72 hours, respectively, and then suspended in 0.8% physiological saline to make bacteria 1 × 10 ⁶ CFU and yeast 1 × 10 ⁵ CFU. 10 g of each emulsion base sample of Examples 5 to 14 and Comparative Examples 1 to 2 were inoculated with 100 µl of four bacterial suspensions and one yeast. Afterwards, each inoculated sample is stored at room temperature, and 1 g of sample is collected under sterile operation every 1 day, 2 days, 3 days, 7 days, 14 days, 21 days and 28 days, and diluted with dilution. Resin agar medium (Letheen agar), yeast was placed on YM agar medium (YM agar) and counted after incubation at 37 ℃, 25 ℃ for 24 to 72 hours. As an antiseptic effect, bacteria should be reduced by more than 99.9% of bacteria within 7 days of inoculation and should not grow during the test period. In addition, yeast and mold should be reduced by at least 90% of bacteria within 7 days of inoculation and should not grow during the test period. The results are shown in Tables 6 to 7 and FIGS.

division Bacterial Count (cells / ml) 3 days 1 week 2 weeks 3 weeks 4 Weeks Example 5 5 x 10 ³ 1 x 10 ³ 1 x 10 ² <100 0 Example 6 6 x 10 ⁵ 2 x 10 ³ 1 x 10 ⁴ 2 x 10 ² <100 Example 7 6 x 10 ⁵ 1 x 10 ⁵ 8 x 10 ² <100 <100 Example 8 3 x 10 ³ 1 x 10 ² <100 <100 0 Example 9 2 x 10 ⁴ 1 x 10 ² <100 0 0 Example 10 ⁴ x 10 ⁴ 2 x 10 ³ 1 x 10 ² 3 x 10 ² <100 Example 11 2 x 10 ² 1 x 10 ² <100 0 0 Example 12 3 x 10 ³ 3 x 10 ² <100 <100 0 Example 13 1 x 10 ² <100 0 0 0 Example 14 2 x 10 ² <100 0 0 0 Example 15 2 x 10 ² <100 0 0 0 Example 16 2 x 10 ⁶ 3 x 10 ^{5 4} x 10 ⁴ 2 x 10 ⁵ 3 x 10 ⁴

As shown in Table 6, in Example 5-10 prepared as an emulsion base formulation, it was found to have very weak antiseptic activity in killing bacteria, but in Examples 13 and 14, all after 7 days It was confirmed to have proper antiseptic activity by killing, and the spectral extract, lotus leaf extract, and cinnamon extract were judged to have excellent antiseptic ability by mutual cooperation.

division Fungal water (cells / ml) 3 days 1 week 2 weeks 3 weeks 4 Weeks Example 5 1 x 10 ^{5 4} x 10 ⁴ 9x10 ² <100 0 Example 6 1 x 10 ⁵ 2 x 10 ⁴ 1 x 10 ⁴ 8 x 10 ² <100 Example 7 6 x 10 ⁴ 2 x 10 ⁴ 2 x 10 ² <100 <100 Example 8 8 x 10 ³ 3 x 10 ³ <100 <100 0 Example 9 7 x 10 ^{4 4} x 10 ⁴ 2 x 10 ² <100 0 Example 10 7 x 10 ⁴ 3 x 10 ⁴ 2 x 10 ² 2 x 10 ² <100 Example 11 4 x 10 ³ 1 x 10 ³ 3 x 10 ² 0 0 Example 12 1 x 10 ⁴ 2 x 10 ³ 8 x 10 ² <100 0 Example 13 3 x 10 ³ 1 x 10 ² <100 0 0 Example 14 2 x 10 ³ 2 x 10 ² <100 0 0 Example 15 6 x 10 ² <100 <100 0 0 Example 16 2 x 10 ⁵ 8 x 10 ⁴ 5 x 10 ⁴ 6 x 10 ⁴ 8 x 10 ³

As shown in Table 7, in Example 5-10 prepared as an emulsion base formulation, it was shown to have very weak antiseptic activity in killing fungi, but in Examples 11, 13, and 14 after 14 days All were killed and showed similar preservative results with the same propensity as the preservative against bacteria of Table 6.

Claims (5)

A cosmetic composition having antibacterial and antiseptic activity comprising 0.2 to 2.0% by weight of the spectral extract, 0.2 to 2.0% by weight of the lotus leaf extract, and 0.2 to 2.0% by weight of the cinnamon extract. According to claim 1, Antimicrobial and antiseptic cosmetic composition further comprises 0.1 to 2.0% by weight red ginseng extract. delete According to claim 1 or 2, wherein the formulation of the cosmetic composition is a softener, milk lotion, nourishing cream, massage cream, essence, cleansing foam, cleansing water, pack, body oil, foundation, lipstick, mascara or makeup base Cosmetic composition having antibacterial and antiseptic activity, characterized in that. delete