KR102271886B1 - Cosmetic composition containing complex medicinal herbs extract for skin whitening and anti-wrinkle effect and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a cosmetic composition having a whitening and wrinkle-improving effect containing an oriental medicinal complex composition as an active ingredient, and more particularly, for a cosmetic composition having a whitening and wrinkle-improving effect, myrrh, lily, pentagram, lower leaf, saengsaeng , sangbaekpi, and oriental medicinal raw materials consisting of angelicae are separately mixed in an extraction solvent, heated and aged; Illite was mixed with an extraction solvent, heated, stirred and aged, and precipitated; Each of the heated and aged raw materials is filtered and centrifuged several times to prepare each herbal extract, and each centrifuged oriental raw material extract is mixed to contain the oriental medicine complex composition as an active ingredient. and a cosmetic composition having a wrinkle-improving effect.
The cosmetic composition according to the present invention includes an oriental medicine complex composition extracted from oriental medicinal raw materials consisting of myrrh, lily, pentagram, lower leaf, sangsaeng, sangbaekpi, angelica, and illite. It has the advantage of providing anti-aging, whitening, wrinkle improvement, and atopic effects.

Description

Functional cosmetic composition containing oriental medicinal complex composition as an active ingredient {COSMETIC COMPOSITION CONTAINING COMPLEX MEDICINAL HERBS EXTRACT FOR SKIN WHITENING AND ANTI-WRINKLE EFFECT AND MANUFACTURING METHOD THEREOF}

The present invention relates to a functional cosmetic composition containing an oriental medicinal complex composition as an active ingredient, and more particularly, the oriental medicinal raw materials consisting of myrrh, lily, obaeja, lower leaf, saengsaeng, sangbaekpi, angelicae are mixed in an extraction solvent and filtered several times and It relates to a cosmetic composition having whitening and anti-wrinkle effects, characterized in that it contains an oriental medicine complex composition prepared by centrifugation and mixing the extract as an active ingredient.

Cosmetics is a high-value-added high-tech industry through the convergence of various industries such as culture, future prospects, and new distribution. It is sensitively affected by changes in income and consumption levels, values, social habits and fashion, etc. Consumers' tendency to pursue well-being as a result of opposition is getting stronger. As the desire for nature increases day by day, the launch of 'natural raw materials' and eco-friendly and naturalistic products is expanding. In line with this social trend, the cosmetic market using herbal ingredients is expanding, and the consumer base is gradually diversifying and segmenting.

As the interest in natural products increases with the tendency to pursue well-being, much effort is being made to search for functional substances from natural products. Also, the use of ingredients with excellent physiological activity of extracts such as wood, leaves, and minerals of trees are used. Methods for use as functional materials are also being actively studied. In particular, there is interest in the development of over-aging skin caused by mental and physical stress, environmental pollution, etc. due to internal and external factors caused by urban industrialization, the preference for premium cosmetics brands by middle-aged people due to an aging population, and delay of skin aging due to the spread of well-being culture. is increasing

Aging is a process of change in which the response to external stimuli is reduced and the ability to maintain homeostasis decreases, making it vulnerable to external stresses, and increases the susceptibility to diseases, which increases the likelihood of being afflicted with chronic diseases. can be defined as As the skin ages, aging also progresses, and wrinkles are the most common symptom of aging skin and are the most common standard for estimating the degree of aging. The causes of aging can be divided into extrinsic factors such as skin damage caused by UV exposure, smoking, drinking, and stress, and intrinsic factors such as genetic factors. Cell damage caused by stress is pointed out as a major mechanism of aging. Active oxygen is pointed out as a major factor in aging, and it is constantly generated in the metabolic process of cells in the body, leading to damage to skin cells and tissues, and putting the skin into an oxidized state. Continued oxidative stress activates proteolytic enzymes that destroy lipid peroxidation, protein oxidation, and interstitial components, and chain cleavage and abnormal cross-linking of collagen and elastin, which are elastic fibers, hyaluronic acid chain cleavage, melanogenesis promotion, DNA oxidation damage to biological components such as In addition, it causes inflammation in the skin and suppresses the skin immune function, increasing the rate of bacterial infection or carcinogenesis, and as a result, accelerates skin aging due to reduced elasticity, wrinkles, blemishes, and freckles. Therefore, in order to delay and prevent skin aging by protecting skin cells and inhibiting damage to connective tissue, it is necessary to suppress the excessive production of active oxygen and efficiently remove the generated active oxygen.

On the other hand, antioxidants are substances that prevent oxidation, and react with active oxygen to remove their toxicity and restore damaged cells in the body. Antioxidants include carotenoids, flavonoids, isoflavones, vitamins, and minerals. Since these antioxidants can be supplied from the outside into the body, research on the development of antioxidants for anti-aging is in progress.

Antioxidants used in cosmetics should be safe for the skin, secure in external conditions, and have excellent compatibility with cosmetic ingredients. However, in the case of natural antioxidants, it is difficult to use the formulation due to its low stability, and in the case of synthetic antioxidants, the antioxidant effect is excellent and the stability in the formulation is excellent, but as mutagenicity and toxicity have recently been highlighted, it is a safer and more active antioxidant derived from natural products. development is required.

Korean Patent Registration No. 10-1537292 Korean Patent Publication No. 10-2019-0036628 Korean Patent Registration No. 10-1207557

An object of the present invention is to solve the above problems, and to provide a cosmetic containing a safe and highly active natural-derived antioxidant. In addition, the present invention provides a cosmetic composition for anti-aging and anti-wrinkle skin moisturizing containing an oriental medicine complex extract using oriental medicinal myrrh, lily, baeja, lower leaf, sangsaeng, sangbaekpi, and angelicae and an active ingredient of illite, a natural mineral. but it has a purpose.

In order to achieve the above object, the present invention for a cosmetic composition having whitening and wrinkle improvement effects, myrrh, lily, obaeja, lower leaf, saengsaeng, sangbaekpi, and oriental medicinal raw materials consisting of angelicae are separately mixed with an extraction solvent and heated, aged; Illite was mixed with an extraction solvent, heated, stirred and aged, and precipitated; Each raw material solvent extract is prepared by mixing it with a solvent and filtering and centrifuging each of the heated and aged raw materials several times, and the raw material solvent extract is extracted by generating water vapor in a vacuum distillation extractor, and then cooled and condensed under reduced pressure for each raw material. A cosmetic composition having whitening, atopic and wrinkle improvement effects, characterized in that it contains an oriental medicine complex composition prepared by mixing each oriental raw material extract prepared including the step of preparing a distillation extract in the same volume ratio as an active ingredient may be doing

The oriental medicine composite composition and the oriental medicine composite composition are characterized in that they contain 0.05 to 80.0% (w/w) based on the total weight of the cosmetic composition.

The cosmetic composition is a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. is composed

The cosmetic product comprising the cosmetic composition according to the present invention is characterized in that it is selected from essence, lotion, skin, mask pack, soap, sun cream, and mist.

The formulation of the cosmetic composition according to the present invention is characterized in that the particle size is 1,500 nm or less.

The cosmetic composition according to the present invention contains an oriental medicinal complex composition extracted from oriental medicinal raw materials consisting of myrrh, lily, quince, lower leaf, saengsaeng, sangbaekpi, and angelicae, and has low skin irritation and stability even after long-term use, and prevents skin aging due to antioxidant action, It has the advantage of providing whitening, wrinkle improvement, and atopic effects.

The cosmetic composition according to the present invention is a formulation having a fine particle size and can increase skin contact area to increase skin efficacy.

1 is a view showing a manufacturing process of an oriental medicine composite composition according to an embodiment of the present invention;
2 is a photograph showing the antibacterial activity of a cosmetic cream, which is a cosmetic product containing the oriental medicinal complex composition of the present invention as an active ingredient;
3 is a photograph showing the efficacy against atopy of a cosmetic cream, which is a cosmetic product containing the oriental medicine complex composition of the present invention as an active ingredient;
4 is a photograph showing the anti-wrinkle effect of a cosmetic cream, which is a cosmetic product containing the oriental medicinal complex composition of the present invention as an active ingredient.

Hereinafter, a preferred embodiment of the present invention will be described in more detail with reference to the drawings. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

The present invention has been devised to achieve the above technical problems, and the cosmetic composition according to the present invention is prepared by the first solvent extraction step and the second reduced pressure distillation extraction step, and includes myrrh, lily, pentagram, lower leaf, and saengsaeng. Step 1-1 of heating and aging by mixing oriental medicinal raw materials consisting of , sangbaekpi, and Angelica keis separately in an extraction solvent consisting of 30% alcoholic ethanol and distilled water mixed in a 1:1 volume ratio;

A first step of mixing 20 parts by weight of illite with 100 parts by weight of an extraction solvent, heating, stirring, aging, and precipitation;

A second step of first filtration of each of the oriental medicinal raw materials according to steps 1-1 and 1-2 to obtain a liquid extract, and first centrifuging the liquid extract after primary filtration to prepare each raw material solvent extract A first solvent extraction step comprising;

a third step of extracting each raw material solvent extract by the first solvent extraction step by heating it in a vacuum distillation extractor at 100 to 200 bar atmospheric pressure and 40 to 60° C. for 0.5 to 5 hours to generate water vapor; and

a fourth step of cooling and condensing the water vapor to -5 to -10 °C to prepare each raw material reduced pressure distillation extract; A second vacuum distillation extraction step comprising a;

It contains the oriental medicine complex composition prepared by mixing each oriental raw material extract prepared including the 1:1:1:1:1:1:1:1 volume ratio as an active ingredient.

1 is a view showing the manufacturing process of the oriental medicine complex composition according to an embodiment of the present invention, Figure 2 is a photograph showing the antibacterial activity of a cosmetic cream, a cosmetic product containing the oriental medicine complex composition of the present invention as an active ingredient, 3 is a photograph showing the efficacy on atopy of a cosmetic cream, which is a cosmetic product containing the oriental medicinal complex composition of the present invention as an active ingredient, and FIG. 4 is a cosmetic product containing the oriental medicinal complex composition of the present invention as an active ingredient. This is a picture showing the improvement effect.

Myrrh is a resin extracted from the stems of Commiphora molmol and other varieties belonging to the olive tree family (Bruseraceae), and has been mainly used in the African continent. Ingredients Essential oils (cuminaldehyde, eugenol, m-cresol, pinene, dipentene, limone, etc.) contain rubber. Myrrh has a scent similar to benzoin, but the size of the lump is uneven, the light is black, and it is said to be used in medicine or consumed by mixing it with hot water. The resin of myrrh contains myrcene, α-camphorene, Z-guggulsterol and essential oils are included. It has been reported that the essential oil component of myrrh exerts cytotoxic and anti-inflammatory effects on fibroblasts of the human gum region, and epithelial cells respond more sensitively to cytotoxicity. Previous studies have reported that myrrh has excellent antibacterial and anti-inflammatory effects.

Recently, efforts to develop various natural chemical substances with antioxidant action as health foods or medicines are increasing. As a result of examining the electron donating ability of myrrh extract, it showed more than 40% self-donating ability at 100mg/L, and 80% at 500mg/L. Excellent antioxidant ability was confirmed by showing more electron donating ability. In addition, as a result of measuring the activity of the extract in the measurement of astringent, 50 mg/L of myrrh extract showed more than 60% activity.

In activated melanocytes, tyrosinase synthesis is promoted and the production of melanin is promoted, which is transported out of the epidermis and discharged, resulting in pigmentation such as spots and freckles. Therefore, the tyrosine activity inhibitor can effectively inhibit the synthesis of melanin in the skin, and the whitening effect can be confirmed through the evaluation of the tyrosinase inhibitory activity. Myrrh extract is 13.7% at 50mg/L, 500mg/L The whitening effect was confirmed with 32.9%.

In the present invention, myrrh is extracted by first extracting the myrrh solvent extract by a solvent extraction method, and the solvent extract is extracted and cooled by generating water vapor in a vacuum distillation extractor to extract the myrrh distillate extract. In general, the vapor pressure of a liquid increases as the temperature increases. The temperature at which the vapor pressure of a liquid equals the external pressure is called the boiling point, and in particular, the boiling point when the external pressure is 1 atmosphere is called the normal boiling point. Common distillation is a method of separating a liquid by heating it to its normal boiling point. When the pressure is lowered using a vacuum pump, etc., the liquid starts to boil at a temperature lower than the normal boiling point, so it is possible to separate the component substances at a low temperature using this. According to the method, it operates at a low temperature, and the residence time in the distillation column is shortened, thus suppressing the decomposition of the product or the formation of unnecessary polymers. Therefore, it is a suitable method for distilling easily decomposed organic compounds or substances that are inconvenient to distill at atmospheric pressure due to their high boiling point. As a solvent extraction method, prepare 20-50 parts by weight of the washed myrrh first, add 2-5 times (w/w) of an extraction solvent, and heat in a shaker incubator at 40°C to 90°C for 1 to 4 hours and then 1 to 4 fermented daily. When the temperature is heated to less than 40 ℃, it is difficult to extract the active ingredient, and when heated to more than 90 ℃, the active ingredient of the oriental medicine material may be destroyed. The aged raw material was first filtered through a 600 mesh filter to remove the residue, then filtered through a 300 mesh filter and then through a 200 mesh filter, and the extract was separated. The extract from which the sludge was removed was centrifuged under the conditions of 4000 rpm and 30 minutes using a centrifuge. The above centrifuged extract was filtered using a 500 mesh filter, and then centrifuged again under conditions of 8000 rpm and 30 minutes to prepare a myrrh solvent extract. By repeating the steps of filtration and centrifugation several times in this way, purification and separation can be performed with high purity suitable for cosmetics.

The myrrh solvent extract is extracted by generating water vapor while heating the myrrh solvent extract at a pressure of 100 to 200 bar atmospheric pressure and 40 ° C to 60 ° C for 0.5 to 10 hours while a cooling condenser is installed to prevent evaporation of the active ingredient, and condensing the water vapor for extraction do. When the heating temperature is set to less than 40° C., the amount of water vapor obtained is poor because the pressure is not reduced, and the active ingredient is destroyed when heated to more than 60° C. The cooling efficiency is set to be -5 ~ -10℃, and for smooth distillation, it is preferable that the bath temperature is 40℃ ~ 60℃. An oriental myrrh extract is prepared through the vacuum distillation extraction step.

Lily (Liliumbrownii F.E. Brownvar. viridulumBaker) is a substance from the lily family, dried perennial herb Lili Bulbus and extensor muscle of a closely related plant. Lily distributed throughout Korea is a cold-resistant hardy bulbous belonging to the family Liliaceae and the genus Lilium, which has been used medicinally and edible. The extracts showed excellent electron donating ability, ABTS scavenging activity, and cell viability. In particular, lily bulbs contain various alkaloids such as colchicine, ferulic acid, p-coumaric acid, sinapic acid, and capsanthin, as well as starch, protein and fat. It has been confirmed that it has the effect of enhancing the physiologically active function.

As a result of confirming the electron donating activity, the electron donating ability of the ethanol extract for each part of the lily was measured. The electron donating ability of the lily (L. davidii) extract was 24.9±5.6% for leaves, 14.9±0.4% for flowers, and 13.798±8.0% for bulbs. L. lancifolium extract was found in the order of leaves 15.1±0.7%, flowers 17.3±0.0%, and bulbs 13.3±0.2%. During photosynthetic metabolism occurring in the chlorophyll of a plant, phenolic and flavonoid substances are produced and accumulated in the leaf more than other parts by the production of polyphenols such as catechins. It is preferable to use it for extraction. Lily extract in the present invention means an extract obtained from stems, branches, roots, leaves, flowers, seeds, etc., of various parts of the Lily of the Lily of the Lily, and preferably an extract extracted from the leaves of the Lily of the Lily.

The lily extract of the present invention was extracted through the first solvent extraction step and the second vacuum distillation extraction step. As a solvent extraction method, prepare 20-50 parts by weight of washed lily leaves first, add 2-5 times (w/w) of an extraction solvent, and heat in a shaking incubator at 40°C to 90°C for 1 to 4 hours and then 1 to Aged for 4 days. The aged raw material was first filtered through a 600 mesh filter to remove the residue, then filtered through a 300 mesh filter and then through a 200 mesh filter, and the extract was separated. The extract from which the sludge was removed was centrifuged under the conditions of 4000 rpm and 30 minutes using a centrifuge. After filtering the above centrifuged extract using a 500 mesh filter, centrifugation was performed again under conditions of 8000 rpm and 30 minutes to extract the lily extract. By repeating the steps of filtration and centrifugation several times in this way, purification and separation can be performed with high purity suitable for cosmetics.

The solvent extract of the lily is extracted by generating water vapor while heating the lily solvent extract at a pressure of 100 to 200 bar atmospheric pressure and 40 ° C to 60 ° C for 0.5 to 10 hours while a cooling condenser is installed to prevent evaporation of the active ingredient, and condensing the water vapor for extraction do. The cooling efficiency is set to be -5 ~ -10℃, and for smooth distillation, it is preferable that the bath temperature is 40℃ ~ 60℃. An oriental medicine lily extract is prepared through the vacuum distillation extraction step.

The lily extract of the present invention includes not only the extract by the above-mentioned extraction method, but also the extract by the supercritical extraction method or ultrasonic extraction method by reduced pressure and high temperature with carbon dioxide. The extraction method by the supercritical extraction method by the reduced pressure by carbon dioxide and the high temperature refers to the supercritical fluid extraction method, and in general, the supercritical fluid is a liquid and a gas having a gas when it reaches a critical point in a high temperature and high pressure condition. Supercritical fluids are used for the extraction of fat-soluble substances due to their properties and chemically similar polarity to non-polar solvents (J Chromatogr A 1998;479:200-205). Carbon dioxide becomes a supercritical fluid with both liquid and gas properties as the pressure and temperature reach the critical point due to the operation of the supercritical fluid device, and as a result, the solubility in fat-soluble solutes increases. When the supercritical carbon dioxide passes through an extraction vessel containing a certain amount of the sample, the fat-soluble substance contained in the sample is extracted into the supercritical carbon dioxide. After extracting the fat-soluble substance, if supercritical carbon dioxide containing a small amount of cosolvent is flowed through the sample remaining in the extraction vessel again, components that were not extracted with pure supercritical carbon dioxide alone can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract the active ingredient by using supercritical carbon dioxide or a mixed fluid in which a cosolvent is additionally mixed with carbon dioxide. As the cosolvent, one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether may be used. Most of the extracted samples contain carbon dioxide, and since carbon dioxide is volatilized into the air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the cosolvent can be removed with a vacuum evaporator.

The extraction solvent that can be used for the ultrasonic extraction method may be one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether. The extracted sample is vacuum filtered to recover the filtrate, then removed by a vacuum evaporator, and an extract can be obtained through a conventional extract preparation method of freeze-drying.

In addition, the lily extract of the present invention includes an extract that has undergone a conventional purification process and/or fermentation process. For example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. obtained through various purification methods additionally carried out It is construed that fractions are also included in the lily extract of the present invention. In addition, the fermented product obtained by fermenting for 1 to 7 days while maintaining pH 5 to 7 under a temperature condition of 20 to 40 ° C. with a bacteria selected from the group consisting of yeast, lactic acid bacteria, Bacillus genus and mixtures thereof is also a lily extract of the present invention. are included in

The extract of the lily of the present invention includes an extract obtained using the poultice method. Poje is a pharmaceutical technology that changes the original properties of medicines by processing them based on oriental medicine theory. It mainly refers to a method of steaming, boiling, roasting, roasting, or heating, or a process in which they are mixed. Treatment conditions are easily selected from 60-100°C, 30 minutes to 6 hours for boiling, 120-180°C for steaming, 10 minutes to 2 hours, and 80-100°C for baking, 10 minutes to 2 hours. In the present invention, it was used after steaming and drying the poplar, and an extract was prepared using this. After extraction by using the method of steaming and drying the poplar, extraction can be performed by not only solvent extraction, but also supercritical extraction using reduced pressure and high temperature with carbon dioxide, or extraction by ultrasonic extraction.

The lily extract of the present invention may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze-drying or spray-drying.

Five baeja (五倍子, Galla rhois) is a worm nest formed by the stab wound of pentaphyllum aphid on the leaves of Rhus javanica Linne or other animals and plants. The appearance is uneven and irregularly divided into 2-4 pockets or broken. The outer surface is grayish grayish brown, covered with light grayish brown short hairs, hard and brittle. By the astringent action of tannin (50-60%), the main component of obaeja, it exhibits antidiarrheal, hemostasis, antibacterial, and glandular secretion inhibitory actions. It has been reported that obaeja has antibacterial, antioxidant, antithrombotic, hepatoprotective and other pharmacological effects, and it has been reported that hot water extract and ethanol extract of obaeja have antibacterial effects against microorganisms isolated from the oral cavity. In particular, the total flavonoid content after fermentation of baeja powder was investigated by Zhishen et al., and it was found to be about 6.5 mg/g, and the total flavonoid content slightly increased after fermentation, and antibacterial activity against bacteria was found in the fermented baeja extract. Since it shows a significant difference according to acidity, it is preferable to use a fermented baeja extract.

The pear extract of the present invention was extracted through the first solvent extraction step and the second vacuum distillation extraction step. As a solvent extraction method, 20 to 50 parts by weight of washed quince is prepared first, 2 to 5 times (w/w) of an extraction solvent is added, and heated at 40°C to 90°C in a shaking incubator for 1 to 4 hours. fermented daily. The aged raw material was first filtered through a 600 mesh filter to remove the residue, then filtered through a 300 mesh filter and then through a 200 mesh filter, and the extract was separated. The extract from which the sludge was removed was centrifuged under the conditions of 4000 rpm and 30 minutes using a centrifuge. After filtering the above centrifuged extract using a 500mesh filter, centrifugation was performed again under conditions of 8000 rpm and 30 minutes to extract the extract of baekjaeja. By repeating the steps of filtration and centrifugation several times in this way, purification and separation can be performed with high purity suitable for cosmetics.

In the state where the cooling condenser is equipped to prevent the active ingredient from evaporating, the solvent extract is heated by heating at a pressure of 100 to 200 bar and 40 ° C to 60 ° C for 0.5 to 10 hours to generate water vapor and extract the water vapor by condensing the extract. do. The cooling efficiency is set to be -5 ~ -10℃, and for smooth distillation, it is preferable that the bath temperature is 40℃ ~ 60℃. Through the vacuum distillation extraction step, an oriental Chinese herbal medicine extract is prepared.

On the other hand, in order to obtain a fermented baekjae extract, one or more of the fruits, leaves, stems, and roots of baejae are collected, washed with purified water and dried. The dried five pear raw materials are mixed with 500 ml of 30% alcoholic ethanol and 500 ml of distilled water, and aged in a shaking incubator for 1 to 4 days. The raw material aged for 1 to 4 days was filtered off with a standard sieve, the extract was separated, and 10 g/L was added to the liquid medium (Potato Dextrose Broth, Accumedia, USA). Saccharomyces cerevisiae was used as the yeast strain used for fermentation, and 50,000 cfu/L per culture was added. The culture was maintained at 30°C and pH 5-7 for 7 days using a 5L fermenter. After the culture was completed, the cultured broth was first removed by centrifugation at 4000rpm for 30 minutes, and then filtered using a 50mesh filter. The filtrate obtained in this way was aged again for about 10 days in a low-temperature warehouse at 4° C., and then the filtered aged solution was filtered again using a 50 mesh filter, and centrifuged again under the conditions of 8000 rpm for 30 minutes to obtain a fermented pear extract. The extraction process through the vacuum distillation extractor will be omitted as the same process.

Lotus (Nelumbo nucifera G.) is a perennial aquatic plant belonging to the Nymphaeaceae family. It is mainly divided into root lotus root, red or white lotus flower, fruit lotus leaf and lotus leaf. It is known to be effective in removing blood clots after childbirth. The physiologically active ingredients isolated from the lotus leaf include alkaloids such as Nelumboside, Nuciferine, and Coclaurine, aromatic acids such as gallic acid and methyl gallate, and quercetin. ), isoquercitrin, hyperoside (hyperoside), rutin (rutin), there are flavonoids such as kaempferol (kaempferol). In the case of lotus leaf extract, it was confirmed that the electron donating ability increased as the concentration increased, and showed an activity of 67.8% at 1,000 μg/ml.

In the case of lotus leaf extract, the tyrosinase inhibitory activity measurement result showed 42.7% at 1,000 μg/ml, confirming that the lotus leaf extract had excellent tyrosinase inhibitory activity. And, as a result of measurement by MTT assay for measuring cell viability, the lotus leaf extract showed a cell viability of 82.2% at 1,000 μg/ml, and it was confirmed that the lotus leaf extract had little cytotoxicity to melanoma cells (B16F10).

On the other hand, excess NO promotes the activity of COX and induces the synthesis of prostaglandin, etc., which is known to intensify the inflammatory response. At a concentration of 25 μg/mL of lotus leaf extract, it is about 67.3%, and at a concentration of 50 μg/mL, 71.2 % of the PGE2 production inhibitory effect, the inhibition of PGE2 production of the lotus leaf extract inhibited NO production, thereby inhibiting the activity of COX, confirming the anti-inflammatory activity of the lotus leaf extract.

As a solvent extraction method, prepare 20-50 parts by weight of washed lotus leaves first, add 2-5 times (w/w) of an extraction solvent, and heat in a shaker incubator at 40°C to 90°C for 1 to 4 hours. fermented daily. The aged raw material was first filtered through a 600 mesh filter to remove the residue, then filtered through a 300 mesh filter and then through a 200 mesh filter, and the extract was separated. The extract from which the sludge was removed was centrifuged under the conditions of 4000 rpm and 30 minutes using a centrifuge. After filtering the above centrifuged extract using a 500 mesh filter, centrifugation was performed again under conditions of 8000 rpm and 30 minutes to extract the lotus leaf extract. By repeating the steps of filtration and centrifugation several times in this way, purification and separation can be performed with high purity suitable for cosmetics.

The lotus leaf solvent extract is extracted by generating water vapor while heating the lotus leaf solvent extract at 100 to 200 bar atmospheric pressure and 40 to 60 ° C. for 0.5 to 10 hours in a state where a cooling condenser is installed to prevent evaporation of the active ingredient, and condensing the water vapor to extract do. The cooling efficiency is set to be -5 ~ -10℃, and for smooth distillation, it is preferable that the bath temperature is 40℃ ~ 60℃. The oriental lotus leaf extract is prepared through the vacuum distillation extraction step. The lotus leaf extract of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

The concentration of the lotus leaf extract is preferably 25 μg/mL or more, and more preferably, a lotus leaf extract having a concentration of 50 μg/mL is used.

The above-mentioned (桑寄生) refers to the leaves, stems, and branches of the mulberry mistletoe (Loranthus para-sticus Merr.) or mistletoe (Viscum album L. var. coloratum Ohwi) belonging to the Loranthaceae family. It contains flavonoid compounds such as avicularin, quercetin, quercitrin and hyperin, and d-catechol. Lectins I, II, and III having molecular weights of 55 kD to 63 kD are isolated from the eucalyptus in Europe, and a lectin having a molecular weight of 60 kD is isolated from the eucalyptus in Korea. In addition, lectins, viscotoxins, and alkaloids of various molecular weights have been isolated. The living lectin I is composed of two different A subunits and B subunits. The A subunit inhibits protein synthesis in the ribosome, the B subunit binds to a galactose residue on the surface of the cell membrane, and the A subunit enters the cell through endocytosis and RTA (ricin A). chain) inhibits protein synthesis in the same way. The biscotoxin isolated from the above organisms activates NK cells.

As a solvent extraction method, 20 to 50 parts by weight of the washed raw material is prepared, 2 to 5 times (w/w) of an extraction solvent is added, and heated at 40°C to 90°C in a shaker incubator for 1 to 4 hours. fermented daily. The aged raw material was first filtered through a 600 mesh filter to remove the residue, then filtered through a 300 mesh filter and then through a 200 mesh filter, and the extract was separated. The extract from which the sludge was removed was centrifuged under the conditions of 4000 rpm and 30 minutes using a centrifuge. The above centrifuged extract was filtered using a 500 mesh filter, and then centrifuged again under conditions of 8000 rpm and 30 minutes to extract the fresh extract. By repeating the steps of filtration and centrifugation several times in this way, purification and separation can be performed with high purity suitable for cosmetics.

The raw solvent extract is extracted by generating water vapor while heating the raw solvent extract at a pressure of 100 to 200 bar at a pressure of 100 to 200 bar and 40 ° C to 60 ° C for 0.5 to 10 hours while a cooling condenser is installed to prevent evaporation of the active ingredient, and condensing the water vapor for extraction do. The cooling efficiency is set to be -5 ~ -10℃, and for smooth distillation, it is preferable that the bath temperature is 40℃ ~ 60℃. Through the vacuum distillation extraction step, an oriental medicine parasitoid extract is prepared.

The raw extract of the present invention may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

Sangbaekpi (桑白皮) refers to the root bark of the mulberry tree (Morus alba Linne) belonging to the mulberry tree (Moraceae). In the Shinnon Bon Chagyeong, the name of Sanggeun Baekpi (桑根白皮) is listed in the middle book. Ingredient Triterpenoid compound, containing α-amyrin and β-amyrin, and β-sitostrol as a steroid compound. is contained. And as a flavonoid, it contains cyclomorucin, a flavonoid compound having an isoprenoid side chain, and kuwanon A, B, C, G, H (kuwanon A, B, C, G, H). .

On the other hand, 1,4-dideoxy-1,4-imino-D-ribitol, one of the pyrrolidine alkaloids isolated from the epidermis, is known to have inhibitory activity against eukaryotic DNA polymerase. In particular, hydroxy-resveratrol contained in the extract of the epidermis has anti-inflammatory and anti-inflammatory properties due to its excellent whitening and antioxidant effects.

As a solvent extraction method, prepare 20 to 50 parts by weight of washed sangbaek skin, add 2 to 5 times (w/w) of an extraction solvent, and heat in a shaker incubator at 40°C to 90°C for 1 to 4 hours and then heat for 1 to 4 days. ripen The aged raw material was first filtered through a 600 mesh filter to remove the residue, then filtered through a 300 mesh filter and then through a 200 mesh filter, and the extract was separated. The extract from which the sludge was removed was centrifuged under the conditions of 4000 rpm and 30 minutes using a centrifuge. After filtering the above centrifuged extract using a 500 mesh filter, centrifugation was performed again under conditions of 8000 rpm and 30 minutes to prepare an epiphyllum extract. By repeating the steps of filtration and centrifugation several times in this way, purification and separation can be performed with high purity suitable for cosmetics.

The extract is extracted by generating water vapor while heating at 100 to 200 bar atmospheric pressure and 40 to 60 ° C. for 0.5 to 10 hours in a state where the cooling condenser is equipped to prevent the active ingredient from being evaporated, and the water vapor is condensed and extracted do. The cooling efficiency is set to be -5 ~ -10℃, and for smooth distillation, it is preferable that the bath temperature is 40℃ ~ 60℃. Through the vacuum distillation extraction step, oriental herbal extracts are prepared.

The extract of Sangbaekpi of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

Angelica gigas Nakai is the root of Angelica gigas Nakai belonging to the Umbelliferae family. Meanwhile, Angelica acutiloba Kitagawa and Chinese Angelica sinenis(Oliv.) Diels are also being distributed. The main ingredients are coumarin, an essential oil and fat-soluble substance, and polysaccharide, a water-soluble substance. Coumarin includes decursin, decursinol anglate, umbelliferone, nodakenin, and feuse. Danone (peucedanone), marmesin (marmesin), dimethyl suberosin (demethylsuberosin), isoimperatorin (isoimperatorin), etc. are included, as a derivative, decursinol (decursinol) is isolated as the main component of Angelica keiskei, active ingredient Decrucinol and decrucinol angelate are isomers with different structures. As flavonoid components, nonane, α-pinene, campheni, methyloctane, lomatin, franmethanol, seselin, guaiacol , butyrolactone. Angelica has anti-carcinogenic, anti-cancer, and anti-cancer properties, as well as antioxidant and active oxygen removal effects by activating blood circulation and metabolism.

At a concentration of 49.89 mg/L of Angelica quai extract, it showed 50% inhibitory effect. This was found to be at a level similar to the IC50 value of arbutin (49.52 mg/L), which is widely used as a whitening raw material, and can reduce melanin synthesis by directly reducing the activity of tyrosinase, which plays an important role in the initial stage of melanin synthesis. It has been confirmed that it is possible In addition, it was confirmed that the melanin content decreased as the concentration of the extract increased, and it was found that the melanin released to the outside of the cell was remarkably decreased. In particular, at a concentration of 0.5 mg/L or more, the content of melanin released to the outside of the cell was reduced by more than 1/3 compared to when treated with a concentration of 0.1 mg/L.

As a solvent extraction method for Angelica keis, prepare 20 to 50 parts by weight of the washed Angelicae, add 2 to 5 times (w/w) of an extraction solvent, and heat it at 40°C to 90°C in a shaking incubator for 1 to 4 hours. Aged for 4 days. The aged raw material was first filtered through a 600 mesh filter to remove the residue, then filtered through a 300 mesh filter and then through a 200 mesh filter, and the extract was separated. The extract from which the sludge was removed was centrifuged under the conditions of 4000 rpm and 30 minutes using a centrifuge. After filtering the above centrifuged extract using a 500 mesh filter, centrifugation was performed again under conditions of 8000 rpm and 30 minutes to extract the Angelica asiatica extract. By repeating the steps of filtration and centrifugation several times in this way, purification and separation can be performed with high purity suitable for cosmetics.

In a state where the cooling condenser is equipped to prevent the active ingredient from evaporating, the Angelica larvae solvent extract is extracted by generating water vapor while heating at a pressure of 100 to 200 bar and 40 ° C to 60 ° C for 0.5 to 10 hours, and condensing the water vapor for extraction. do. The cooling efficiency is set to be -5 ~ -10℃, and for smooth distillation, it is preferable that the bath temperature is 40℃ ~ 60℃. Through the vacuum distillation extraction step, an oriental herb Angelica extract is prepared.

The Angelica asiatica extract of the present invention may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

20 parts by weight of Illite is mixed with 100 parts by weight of the extraction solvent, heated in a shaking extractor at 90 to 120° C. for 1-2 hours, stirred and aged for 1 to 4 days, and extracted by precipitation. At this time, it is aged in a sealed state so that no air is introduced during the stirring process. After aging, it is filtered several times to remove the residue. First, filtered through a 600mesh filter, 300mesh, 200mesh filtered, and the extract from which the residual sludge is removed is first centrifuged under the conditions of 4000rpm and 30 minutes using a centrifuge.

After filtering the first centrifuged extract using a 500 mesh filter, the second centrifugation was performed at 8000 rpm for 30 minutes to extract the illite solvent extract. By repeating the steps of filtration and centrifugation several times in this way, purification and separation can be performed with high purity suitable for cosmetics.

The illite solvent extract is extracted by generating water vapor while heating the illite solvent extract at a pressure of 100 to 200 bar atmospheric pressure and 40 ° C to 60 ° C for 0.5 to 10 hours while a cooling condenser is installed to prevent evaporation of the active ingredient, and condensing the water vapor extract The cooling efficiency is set to be -5 ~ -10℃, and for smooth distillation, it is preferable that the bath temperature is 40℃ ~ 60℃. Through the vacuum distillation extraction step, the illite extract is prepared.

Illite is a representative natural clay material and has the property of adsorbing heavy metals, radionuclides, and harmful gases appropriately. Pure illite is generally white, but when it contains a large amount of iron, it is brown and contains a small amount. In some cases, it has a pale yellow color. It is known that Illite effectively removes oil and toxic substances from the skin. Clays act on the skin to remove toxins by cation exchange properties, react with organic matter to adsorb waste, reduce inflammation by antibacterial action, and clean It has effects such as keratin whitening, antioxidant, metabolic activity, blood circulation, and moisture control.

illite Bulk density (g/ml) 0. 3～0. 8 particle size ( ㎛) 1 to 15 importance 2. 7 pH 7. 0～9. 0 (neutral) Specific surface area ( m²/g) 2~15 Oil absorption ( ㎖ / 100g) 30-50

As shown in Table 1, Illite has a bulk density of 0.3 to 0.8 g/ml, which is the volume occupied by 1 g of solid, and as a cosmetic raw material, Illite has a bulk degree of 0.5 to 0. 8 g/ml, particle size 3~15 ㎛, specific surface 3~15m²/g, oil absorption is preferably 30~40ml/100g.

The illite crystal has a 2:1 layer structure in which one Al(O,OH) _{6 octahedral sheet is sandwiched between two SiO 4} tetrahedral sheets, and the illite crystal structure is Since Si ⁴⁺ is ^{substituted by Al 3+ , K+} exists between the layers to balance the charge. Illite crystals have a developed cleavage plane, which is negatively charged because oxygen (O) exists on the cleavage plane, and positive and negative charges are present on the ^{edge perpendicular to the cleavage plane because O -} and (OH) ^{- exist.} exists Rhombohedral O ⁻ and (OH) ⁻ act as ligands in cationic surface complexation. As such, the surface of the illite particles is negatively charged, and this surface charge plays an important role in the adsorption of positive ions.

Illite is known for its whitening, skin purification, antibacterial, deodorizing, moisturizing, skin protection, cellulite decomposition, and cell activation effects. It is non-toxic to the skin and has the effect of purifying the skin by adsorbing sebum or organic substances, and it has the effect of decomposing cellulite through the cationic effect. It emits far-infrared rays that help cell activation, and has functions such as blocking ultraviolet rays and adsorbing heavy metal wastes. Therefore, it is used as a raw material for functional cosmetics such as atopy and acne.

ingredient contain Efficacy and effectiveness SiO₂silicon oxide) O waste and sebum removal Al (aluminum) O Promote blood circulation and metabolism, remove waste products Fe₂O₃ (iron oxide) O Collagen binding Ca (calcium) O detoxification, stability Na (sodium) O Osmotic pressure, water balance Mg (magnesium) O Promotion of waste excretion, collagen binding K (sword) O Osmotic pressure control, moisture balance, soothing effect S (sulfur) O anti-inflammatory, antibacterial Ti (titanium) O Whitening Pb (lead) X Preservative (heavy metal) Cd (cadnium) X

Table 2 above shows the effects of the components of Illite on the skin. Illite can be added by processing into powder or granular form, and processed into fine powder of 1000 mesh or more and used in cosmetics. It can be used as granular particles of 1000 mesh or more for the same purpose as cosmetic packs.

The myrrh extract, lily extract, baeja extract, lower leaf extract, said fresh extract, sangbaekpi extract, Angelica asiatica extract and illite extract were mixed in a 1:1:1:1:1:1:1:1 volume ratio to prepare a herbal complex composition. do.

The oriental medicine composite composition contains 0.05 to 80.0% (w/w) based on the total weight of the cosmetic composition, and more preferably 0.1 to 20.0% by weight. At this time, when the total weight of the oriental medicine composite composition is less than 0.05% by weight, the effect is difficult to appear, and when it exceeds 80.0% by weight, there is a problem of price increase and may affect the stabilization of the formulation.

On the other hand, the formulation of the cosmetic composition according to the present invention is characterized in that the particle size is 1,500 nm or less. It is a formulation to increase the efficiency of the functional oriental medicinal complex composition with secured stability, and it is desirable to have an emulsified particle size of 1,500 nm or less. The finer the particle size, the larger the area in contact with the skin surface, so that the skin efficiency of the oriental medicine complex composition is increased. can

The ingredients included in the cosmetic composition of the present invention are active ingredients, and various ingredients that maintain the activity of biocomponents in addition to the oriental medicine complex composition, such as preservatives, osmotic agents, pH adjusters, buffers, stabilizers, aluminum hydroxide, aluminum phosphate, interface Active agents, liposomes, thickeners, etc. and ingredients commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, conventional adjuvants such as vitamins, pigments and fragrances, and carriers, and the like, but are not limited thereto. does not

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing agent , oil, powder foundation, emulsion foundation, wax foundation, spray, etc., but is not limited thereto.

When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, and water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

More preferably, the cosmetic composition may be prepared as a cosmetic product containing the same, and may be one product selected from essence, lotion, skin, mask pack, soap, shampoo, cream, and mist. Specifically, the cosmetic composition of the present invention is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence, It may include formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions, and body cleansers.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Myrrh, lily, Obaeja, lower leaf, myrrh, sangbaekpi, angelica, each of the herbal raw materials consisting of myrrh extract, lily extract, pentagram extract, lower leaf extract, said fresh extract, sangbaekpi extract, angelicae extract and illite extract are 1:1:1, respectively. By mixing :1:1:1:1:1 (w/w), an oriental medicine composite composition was prepared.

A cosmetic cream was prepared as a cosmetic product containing the oriental medicine complex composition of Example 1 as an active ingredient.

[Comparative Example 1]

In contrast to Example 2, a cosmetic cream was prepared as a cosmetic product containing the remaining oriental raw material extracts except for Illite in the oriental medicinal complex composition of Example 1 as an active ingredient.

[Experimental Example 1]

The moisture content measurement experiment of the cosmetic composition containing the oriental medicine complex composition of Example 1 as an active ingredient was performed and shown in Tables 3 and 4.

(Mean±SD) visit illite 0 weeks 54.34±11.67 2 weeks 59.74±12.49 4 weeks 60.59±10.69 6 weeks 65.27±8.87 Within comparison

probability of significance 0 weeks - 2 weeks 0.0008* Weeks 0-4 0.028 Weeks 0-6 0.011* Between groups probability of significance 0 weeks - 2 weeks 1.000 Weeks 0-4 0.796 Weeks 0-6 0.489

*:p<0.05 Friedman test, p<0.017 Wilcoxon signed-rank test with a bonferroni method

As shown in Table 3, the control group using Illite showed 54.34±11.67 at week 0, 59.74±12.49, 60. 59±10.69, and 65.27±8.87 at week 2, 4 and 6, respectively, in both the experimental group and the control group. It could be seen that the skin moisture content level gradually increased with the lapse of the experimental period, and a statistically significant difference was found with p<0.017 (=5%/3) after 6 weeks of use in both groups compared to use. there was

product 2 weeks after use 4 weeks after use after 6 weeks of use illite 9.94 11.51 20.12

The table above shows the change in skin moisture content (%) after using / after using the product of Example 1, and it was confirmed that the moisture content significantly increased after using the cosmetic composition containing the oriental medicinal complex composition containing illite as an active ingredient. .

[Experimental Example 2]

An experiment for measuring the number of skin pores of a cosmetic cream containing the oriental medicinal complex composition of Example 2 as an active ingredient was conducted and shown in Tables 5 and 6.

(Mean±SD) visit illite 0 weeks 12.44 ±13.43 2 weeks 11.78±12.00 4 weeks 11.44±12.62 6 weeks 11.33±11.58 Within comparison probability of significance 0 weeks - 2 weeks 0.339 Weeks 0-4 0.865 Weeks 0-6 0.394 Between groups probability of significance 0 weeks - 2 weeks 0.024 ^† Weeks 0-4 0.014 ^† Weeks 0-6 0.014 ^†

†:p<0.05 Mann-Whitney U test

This table shows the results of skin pore count measurement. In the control group using Illite, it was 12.44±13.43 at week 0, 11.78±12.00, 11.44±12.62, and 11.33±11.58 at week 2, 4 and 6, respectively, indicating that the number of skin pores decreased. Therefore, the number of pores in the experimental group was p<0 after 2, 4, and 6 weeks of use compared to before use. 017 (=5%/3) significantly decreased, and no significant difference was found in the control group.

product 2 weeks after use 4 weeks after use after 6 weeks of use illite -5.3 6 -8.04 -8.93

Table 6 shows the change rate (%) in the number of skin pores after using the product of Example 2, and it was confirmed that the pores were significantly reduced after using the cosmetic composition containing the oriental medicinal complex composition containing illite as an active ingredient. .

[Experimental Example 3]

The antibacterial effect test of the present invention confirmed the bactericidal action of the natural extract against acne bacteria (Propionibacterium acnes), a representative inflammatory substance.

Pacnes KCTC3314 was used as a strain for measuring the antibacterial activity of Acne acnes, and the strain was cultured using an anaerobic broth MIC (Anaerobic broth MIC). Add 01 mL of the Pacnes KCTC3314 seed culture medium cultured for 2 days to the anaerobic medium MIC and smear it so that it becomes 1~5×10.8 CFU/mL. was used and incubated at 35°C. Disc-paper (diameter 6mm, Whatman No2) was placed on the culture medium in which the acne strain was cultured, the ointment of the present invention was diluted 100-fold, 01 mL was injected into the disc-paper, and the size of the growth inhibition ring was measured and evaluated. As a control, ampicillin was used. As a result of the experiment, as shown in FIG. 3 , the cosmetic cream as a cosmetic product containing the oriental medicine complex composition of the present invention as an active ingredient exhibited high antibacterial activity against acne bacteria.

In FIG. 2, a is the epidermis, b is the dermis, and c is the subcutaneous fat layer, (1) is a diagram showing a state in which the sebaceous glands are exaggerated by acne bacteria and the pores are clogged, and (2) is the oriental medicine complex composition of Example 2 It shows a state in which a cosmetic product prepared with a cosmetic composition containing as an active ingredient is applied, and is absorbed into the epidermis and dermis as well as the surface of the skin to have a sterilizing action.

[Experimental Example 4]

A cream (Example 2) containing a cosmetic composition containing the oriental medicine complex composition of Example 1 as an active ingredient was prepared, and the efficacy against atopy was evaluated.

The cream containing the oriental medicine complex composition of the present invention was externally applied to the symptomatic site 3 times a day for 6 atopic patients for 2 weeks. The subjects of this study, atopic patients, were mostly patients with atopic symptoms including red spots, erosions, lichenification, lice, and itching before treatment, and they varied in gender, age, history of atopic dermatitis, and symptom sites. As a result of the experiment, as shown in Table 7, it was confirmed that the cream containing the oriental medicine complex composition of the present invention relieved atopy.

atopic patient
gender/military history atopic patient
symptom area/severity Cream formulation containing oriental medicinal complex composition of the present invention Female/ 10 years whole body/medium improved Female/ 7 years face / middle improved Female/ 5 years arms, legs/middle improved Male/ 7 years arm / top improved Male / 6 years face, neck/middle improved Male / 5 years whole body/medium improved

After external application of the cream containing the oriental medicine complex composition of the present invention to atopic patients, the conditions before (1) and after (2) are compared and shown in FIG. 3 to confirm that atopy is improved. Through this, it can be seen that the cream containing the oriental medicine complex composition of the present invention relieves atopy.

[Experimental Example 5]

The formulation stability of a cosmetic cream comprising a cosmetic composition containing the oriental medicine complex composition of Example 2 as an active ingredient was tested. Using distilled water in Haye's test chamber, adjust the concentration of the herbal complex composition of Example 2, 1%, 3%, and 5%, and attach the patch to the inside of the left arm for 24 hours and then peel off the blank and the skin reaction of the test product ( Erythema, edema, etc.) was observed and evaluated (Closed patch test using Haye's test Chamber). The test was repeated twice in total.

Judgment was made a total of 4 times over 30 minutes, 1 day, 2 days, and 3 days after patch removal, and was determined based on Draize's P.I.I method.

age gender skin type Abnormality One 25 female complexity clear 2 38 female Intelligence clear 3 45 female sensitivity clear 4 36 female inattention clear 5 28 female Intelligence clear 6 31 male Intelligence clear 7 35 male Intelligence clear 8 36 male sensitivity clear 9 41 male inattention clear 10 27 male Intelligence clear

As shown in Table 8 above, it was confirmed that all of the 10 people were not abnormal.

[Experimental Example 6]

The skin wrinkle improvement effect of the oriental medicinal complex composition of the present invention was evaluated through an actual use test. A nutrient cream (Example 2) containing 3% (v/v) of the oriental medicine complex composition of Example 1, respectively, and a cream in which the oriental medicine complex composition was replaced with purified water in the nutrient cream as Comparative Example 1, Examples A cream in which the oriental medicinal complex composition of 1 was replaced with adenosine, a substance known to have wrinkle improvement effects, was prepared in Comparative Example 4. Among 30 women aged 30-40, women who engage in external activities were randomly divided into 3 groups, and after washing the cream of Example 2 and Comparative Example 1 and Comparative Example 2 twice daily in the morning and evening, apply an appropriate amount of the cream around the eyes. It was corrected continuously for 2 months. The wrinkle improvement effect of each subject was evaluated through visual observation, and the effective rate (%) was obtained by dividing the number with excellent or slight skin wrinkle improvement effect by the total number of subjects as a percentage, and the results are shown in Table 9 below. It was.

Skin wrinkle improvement effect Effectiveness (%) Great middle none Example 2 25 2 3 90.00 Comparative Example 1 3 One 26 13.33 Comparative Example 2 24 3 3 90.00

According to the results of Table 9, the cosmetic containing the oriental medicinal complex composition of the present invention not only showed an excellent skin wrinkle improvement effect compared to the cream of Comparative Example 3, but also contained adenosine, a component known to have a wrinkle improvement effect. It exhibited a similar effect to the cream of Comparative Example 4.

On the other hand, before (1) and after (1) and after ( 2) The states were compared and shown in FIG. 5 . Through this, it can be seen that the cream containing the oriental medicine complex composition of the present invention improves wrinkles.

The present invention is a cosmetic composition comprising an oriental medicine complex composition extracted from oriental medicinal raw materials consisting of myrrh, lily, pentagram, lower leaf, saengsaeng, sangbaekpi, angelica, and illite. It has the effect of preventing, whitening, wrinkle improvement, and atopy improvement.

Claims (6)

Myrrh, lily, baeja, lower leaf, saengsaeng, sangbaekpi, and angelica, respectively, separately mixed with 30% alcohol and distilled water in a 1:1 volume ratio in an extraction solvent, heated and aged;
A first step of mixing 20 parts by weight of illite with 100 parts by weight of an extraction solvent, heating, stirring, aging, and precipitation;
Preparation of each raw material solvent extract by primary filtration of each of the herbal raw materials according to steps 1-1 and 1-2 to obtain a liquid extract, and primary centrifugation after primary filtration of the liquid extract Step 2; a first solvent extraction step comprising;
A third step of extracting each raw material solvent extract by the first solvent extraction step by heating in a vacuum distillation extractor at 100 to 200 bar atmospheric pressure and 40 to 60 ° C. for 0.5 to 10 hours to generate water vapor; and
a fourth step of cooling and condensing the water vapor to -5 to -10° C. to prepare each raw material reduced pressure distillation extract; A second vacuum distillation extraction step comprising a;
Contains an oriental medicine complex composition prepared by mixing each oriental raw material extract prepared including the 1:1:1:1:1:1:1:1 volume ratio as an active ingredient,
The first step 1-1 is to be heated and aged at 40 ~ 90 ℃ for 1 ~ 4 hours,
The first 1-2 step is a functional cosmetic composition, characterized in that it is heated and aged at 90 ~ 120 ℃ 1 ~ 2 hours. delete The method according to claim 1,
The oriental medicine composite composition is a functional cosmetic composition, characterized in that it contains 0.05 to 80.0% (w / w) with respect to the total weight of the cosmetic composition. The cosmetic composition according to claim 1 is
characterized in that it has a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. A functional cosmetic composition. A cosmetic product comprising the cosmetic composition according to claim 1, wherein the product is one selected from essence, lotion, skin, mask pack, soap, shampoo, cream, and mist. 5. The method according to claim 4,
The formulation of the cosmetic composition is a cosmetic composition, characterized in that the particle size is 1,500 nm or less.

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