KR101381755B1 - Method for producing cryptomeria japonica extracts and cosmetic composition containing the same - Google Patents

Abstract

The present invention relates to the production of cedar extracts that can be used as a raw material of cosmetics, and specifically to the production of cedar extracts having excellent antioxidant effect, anti-wrinkle and moisturizing effect and expression inhibitory activity of inflammatory mediators by removing inactive polysaccharides. It is about. According to the present invention (A) adding a solvent of 5 to 10 times the weight to the dried cedar leaves or stems to extract hot water at 70 ~ 90 ℃; (B) filtering and recovering the obtained hot water extract, and concentrating under reduced pressure to 1/10 to 1/3 of the initial volume; (C) adding 2-6 times the volume of ethanol to the final concentrate and stirring until precipitation occurs; And (D) there is provided a method of producing a cedar extract, characterized in that it comprises the step of filtering the precipitate.

Description

Method for producing a cedar extract from which the inactive polysaccharide is removed and a cosmetic composition comprising the same {Method for producing cryptomeria japonica extracts and cosmetic composition containing the same}

The present invention relates to the production of cedar extracts that can be used as a raw material of cosmetics, and specifically to the production of cedar extracts having excellent antioxidant effect, anti-wrinkle and moisturizing effect and expression inhibitory activity of inflammatory mediators by removing inactive polysaccharides. It is about.

Cosmetics begin with pursuit of cleanliness and beauty, and are active due to external factors such as moisture, pollutants and stress, ultraviolet rays, and internal factors such as deactivation of immune cells and deterioration of cell activity. It has been extended to eliminate or suppress the cause of skin aging such as destruction of proteins, nucleic acids, and cell membrane lipids by oxygen and peroxides.

Among them, cosmetics for wrinkle improvement and suppression have received much attention from consumers who want to maintain youth. Wrinkles are formed by repeated movement of muscles over a long period of time in a specific direction, which is affected by age, external environment, ultraviolet radiation, and the like. Damage to skin cells caused by these factors causes various aging phenomena. Collagen and elastin, which constitute the dermal matrix, are not easily produced and degraded. Thus, the firmness of the matrix is reduced and the elasticity of the skin is reduced and wrinkles are generated. The mechanism of photoaging is known to decrease collagen synthesis by UV irradiation and increase the expression of matrix metalloproteinase, which leads to increased degradation of collagen and elastin, which are the major constituents of the substrate, and thus photoaging including wrinkles. have. Elastase is also an enzyme that decomposes elastic fibers in the decomposition process of elastic fibers, and its activity is increased by irradiation with ultraviolet rays, which is involved in photoaging phenomenon.

In addition, attempts have been made to alleviate immune responses by controlling inflammatory mediators and to relieve skin irritation through skin barrier strengthening and skin protection. Recently, many studies have been published to search for cosmetics based on safe natural materials to improve skin wrinkles and relieve skin irritation and inflammation.

As an example, Korean Patent Application Publication No. 2011-0024657 discloses a cosmetic composition for improving wrinkles that contains an extract of Callistemon lanceolatus, and Korean Patent Application Publication No. 2011-0110069 discloses a skin containing a ginger tree leaf extract. A cosmetic composition for whitening and anti-wrinkle is disclosed, and Korean Patent Publication No. 2011-0120813 discloses a cosmetic composition for anti-wrinkle containing Euphorbia supina extract as an active ingredient. In addition, the Republic of Korea Patent Publication No. 2011-0047374 contains cedar extract and bamboo extract showing an excellent effect on the skin moisturizing and at the same time suppress the expression of inflammatory mediators to improve male skin showing the effect of skin irritation and inflammation caused by acne The cosmetic composition is disclosed, Korean Patent No.1050613 contains low-temperature ultrasonic extraction of plants consisting of bamboo, pine, cedar, birch, citrus and camphor tree with low-temperature ultrasonic extraction and excellent antioxidant and anti-wrinkle and moisturizing effect A cosmetic composition is disclosed.

Therefore, the present inventors have studied the preparation of natural cosmetics having the effect of reducing wrinkles, moisturizing and inflammatory parameters, and confirmed that the activity is greatly improved by removing the inactive polysaccharide from the cedar extract by a specific method. The invention was completed.

Therefore, it is an object of the present invention to provide a method of preparing cedar extracts having excellent antioxidant, anti-wrinkle and moisturizing effects and expression inhibitory activity of inflammatory mediators by removing inactive polysaccharides in the preparation of cedar extracts.

In another aspect, the present invention is to provide a cosmetic composition prepared by the production method exhibiting excellent anti-wrinkle, moisturizing and salt-containing effect.

In order to achieve the above object, according to the present invention comprising the steps of preparing a cedar crude extract; And adding ethanol to the crude extract to provide a method for producing cedar extracts comprising the step of removing inactive polysaccharides. The cedar crude extract can be obtained by hot water extraction, solvent extraction or ultrasonic extraction. Specifically, (A) adding a solvent of 5 to 10 times the weight to the dried cedar leaves or stems and extracting hot water at 70 ~ 90 ℃;

(B) filtering and recovering the obtained hot water extract, and concentrating under reduced pressure to 1/10 to 1/3 of the initial volume;

(C) adding 2-6 times the volume of ethanol to the final concentrate and stirring until precipitation occurs; And

(D) there is provided a method for producing a cedar extract comprising the step of filtering out the precipitate.

In order to achieve the above another object, according to the present invention there is provided a cosmetic composition containing 0.001 to 50% by weight of cedar extract prepared by the extraction method.

According to the extraction method of the present invention, since the inactive polysaccharide contained in the cedar extract is efficiently removed, the cedar extract having excellent antioxidant effect, anti-wrinkle and moisturizing effect, and inhibitory activity of expression of inflammatory parameters is obtained.

Hereinafter, the present invention will be described in detail.

Japanese redwood (peacock pine) used in the present invention is an evergreen conifer tree belonging to the genus Cryptomeria. It is used for timber and is native to eastern Asia. It grows over 45m tall and has a circumference of 4.5-7.5m. The tree is shaped like a pyramid, with branches growing tightly around the trunk and stretching to the side. Wood is scented and reddish brown and is used to make boats, houses, bridges, furniture, vats, and decorative sculptures. Leaves make incense. Cedar contains phytoncite, a substance that plants release to resist pests, germs, and bacteria, and has been widely used to cure harmful bacteria since ancient times. Specifically, allergic skin such as boils, swelling and atopy It has been widely used for disease improvement. Accordingly, cosmetics containing conventional cedar extracts have been developed. By the way, the cedar extract was prepared by hot water extraction, solvent extraction, ultrasonic extraction. By the way, the extract contains inactive polysaccharides (inactive polysaccharides), but it is difficult to remove it has been used as it is. This required the use of large amounts to achieve the required effect, which was toxic. The present invention is characterized by enhancing its activity by removing such inactive polysaccharides by a simple and efficient method.

According to one embodiment of the invention, (A) adding a solvent of 5 to 10 times the weight to the dried cedar leaves or stems to extract the hydrothermal at 70 ~ 90 degrees; (B) filtering and recovering the obtained hot water extract, and concentrating under reduced pressure to 1/10 to 1/3 of the initial volume; (C) adding 2-6 times the volume of ethanol to the final concentrate and stirring until precipitation occurs; And (D) there is provided a method for producing a cedar extract comprising the step of filtering out the precipitate.

The leaves, stems or mixtures of the cedars can be used, preferably the leaves of cedar. In this case, a solvent selected from water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, chloroform, hexane, acetonitrile, or a mixed solvent thereof may be used as the solvent, and preferably water or ethanol is used. 5-10 times the weight of the solvent is added to the dried cedar leaves or stems and extracted with hot water at 70-90 degrees. Filtration and recovery of the obtained hot water extract, and concentrated under reduced pressure to 1/10 ~ 1/3 of the initial volume to prepare a cedar hot water extract. Since the hydrothermal extract contains an inactive polysaccharide, in order to remove it, 2-6 times the volume, preferably 4-6 times the volume of ethanol is added and stirred. Upon stirring, the inactive polysaccharide is removed by precipitation. The cedar extract having excellent activity is prepared by filtering and removing the precipitate produced by the precipitation reaction and removing the residual solvent.

The cedar extract thus prepared was tested in comparison with the hydrothermal extract, and showed a superior anti-aging effect in the type I procollagen production assay. In addition, the cedar extract exhibited an improvement in skin wrinkles by relieving the collagen reduction phenomenon in the skin by significantly inhibiting the biosynthesis of the tissue decomposing enzyme MMP-1, compared to the hydrothermal extract. Also in the DPPH free radical scavenging activity, the cedar extract according to the present invention showed a similar degree of DPPH free radical scavenging activity to BHT. Furthermore, cedar extract according to the present invention was confirmed to inhibit the inflammatory response by concentration-dependently reducing the biosynthesis of TNF-α and COX-2 induced by the inflammatory trigger factors LPS (Lipopolysaccharide), and compared with the skin extract It effectively inhibits the inflammatory response, thereby easing the skin trouble.

According to an embodiment of the present invention there is provided a cosmetic composition containing 0.001 to 50% by weight of the cedar extract prepared by the extraction method. The cedar extract has excellent antioxidant activity, moisturizing and anti-wrinkle activity, and has an activity of inhibiting the expression of inflammatory mediators, so it can be usefully used for whitening, wrinkle improvement, cosmetics for acne and the like. The cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing It can be applied in the form of foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder, eye shadow and the like.

[Example]

Hereinafter, the present invention will be described in more detail based on the following Examples and Test Examples. However, the following examples are only for illustrating the present invention, and the present invention is not limited by the following examples.

Example 1: Preparation of Cedar Extract

1 kg of dry weight cedar leaves were dried and pulverized, 10 kg of ethanol was added thereto, stirred at room temperature for 24 hours, and then centrifuged to remove the solvent. The solvent-free cedar leaves were collected and dried, followed by hot water extraction at 90 ° C. for 6 hours, and then the obtained hot water extract was filtered through 200 mesh and concentrated under reduced pressure at 50 ° C. to obtain 1/3 of the initial volume. Then, two times the volume of ethanol was added to the concentrate, followed by stirring at a rate of 50 rpm to perform ethanol precipitation. After filtration and recovery using a filter press to remove the precipitated cedar polysaccharides, all residual solvents were removed and dried in vacuo to obtain 24 g of cedar extract in powder form.

Example 2: Preparation of Cedar Extract

1 kg of dry weight cedar leaves were dried and pulverized, 10 kg of ethanol was added thereto, stirred at room temperature for 24 hours, and then centrifuged to remove the solvent. The solvent-free cedar leaves were collected and dried, followed by hot water extraction at 90 ° C. for 6 hours, and then the obtained hot water extract was filtered through 200 mesh and concentrated under reduced pressure at 50 ° C. to obtain 1/3 of the initial volume. Subsequently, 4 times the volume of ethanol was added to the concentrate, followed by stirring at a rate of 50 rpm to perform ethanol precipitation. After filtration and recovery using a filter press to remove the precipitated cedar polysaccharides, all residual solvents were removed, followed by vacuum drying to obtain 53 g of cedar extract in powder form.

Example 3: Preparation of Cedar Extract

1 kg of dry weight cedar leaves were dried and pulverized, 10 kg of ethanol was added thereto, stirred at room temperature for 24 hours, and then centrifuged to remove the solvent. The solvent-free cedar leaves were collected and dried, followed by hot water extraction at 90 ° C. for 6 hours, and then the obtained hot water extract was filtered through 200 mesh and concentrated under reduced pressure at 50 ° C. to obtain 1/3 of the initial volume. Subsequently, 6 times the volume of ethanol was added to the concentrate, and the mixture was stirred at a speed of 50 rpm to perform ethanol precipitation. After filtration and recovery using a filter press to remove the precipitated cedar polysaccharides, all residual solvents were removed, followed by vacuum drying to obtain 72 g of cedar extract in powder form.

Comparative Example 1: Preparation of Cedar Extract

1 kg of dry weight cedar leaves were dried and pulverized, 10 kg of ethanol was added thereto, stirred at room temperature for 24 hours, and then centrifuged to remove the solvent. After collecting and drying the solvent-free cedar leaves, hot water extract for 6 hours at 90 ℃, then filtered and recovered the obtained hot water extract with 200 mesh, all residual solvents are removed by vacuum drying and then cedar extract in powder form 205 g was obtained.

Test Example 1: Type I procollagen assay

Human normal fibroblasts were cultured in a 96-well plate incubator at a concentration of 1 × 10 ⁵ , and then the three cedar extracts obtained in Examples 1 to 3 were replaced with a medium containing 10 ppm, 100 ppm, and 500 ppm. TGF-β (Human Transforming Growth Factor-β1) was used as a positive control. Cells were harvested on the third day of culture to quantify the amount of type I procollagen produced by ELISA. The amount of procollagen of the control group without the cedar extract of the above example was set to 0 and the positive control group was set to 100, and the comparison with each measured value was calculated. The results are shown in Table 1 below.

division Type I procollagen biosynthesis (%) Negative control group 0 Comparative Example 1

Hot Water Extract (10ppm) 2 Hot Water Extract (100ppm) 11 Hot Water Extract (500ppm) 21 Example 1

2-fold ethanol (10 ppm) 5 2-fold ethanol (100 ppm) 12 2-fold ethanol (500 ppm) 22 Example 2

4-fold ethanol (10 ppm) 14 4-fold ethanol (100 ppm) 39 4-fold ethanol (500 ppm) 42 Example 3

6-fold ethanol (10 ppm) 19 6-fold ethanol (100 ppm) 43 6-fold ethanol (500 ppm) 54 Positive control group TGF-β (10ng / ml) 100

As confirmed in Table 1, the cedar extracts of Examples 1 to 3 in human normal fibroblast culture experiments promoted type I procollagen production in a concentration-dependent manner compared to the negative control group. In particular, Example 2 and Example 3 showed a more excellent effect. Therefore, it can be seen that the cedar extract of the present invention exhibits an anti-aging effect by alleviating collagen reduction in the skin by promoting collagen production. In addition, the experiments treated with the same concentration as the hydrothermal extract was able to confirm the superior anti-aging effect.

Test Example 2: MMP-1 biosynthesis inhibition assay

After culturing human normal fibroblasts at a concentration of 1 X 10 ⁵ in a 12-hole plate incubator and irradiating UVB at 40 mJ / cm ² , the cedar extracts obtained in Examples 1 to 3 and Comparative Example 1 were 10 ppm and 100 ppm, respectively. , Was replaced with a medium containing a concentration of 500ppm. Retinoic acid was used as a positive control. Cells were harvested on the second day of culture to quantify the amount of MMP-1 (matrix metalloproteinase I) produced by ELISA. A comparison value with the measured value was calculated using 100 as the control group which did not include the cedar extract of Example and was not irradiated with ultraviolet rays. The results are shown in Table 2 below.

division MMP-1 biosynthesis (%) Control group
No UV irradiation 100 UV irradiation 154 Comparative Example 1

UV irradiation + hot water extract (10ppm) 155 UV irradiation + hot water extract (100ppm) 140 UV irradiation + hot water extract (500ppm) 131 Example 1

UV irradiation + 2 times ethanol (10 ppm) 151 UV irradiation + 2 times ethanol (100 ppm) 142 UV irradiation + 2 times ethanol (500 ppm) 129 Example 2

UV irradiation + 4 times ethanol (10 ppm) 131 UV irradiation + 4 times ethanol (100 ppm) 110 UV irradiation + 4 times ethanol (500 ppm) 101 Example 3

UV irradiation + 6 times ethanol (10 ppm) 132 UV irradiation + 6 times ethanol (100 ppm) 109 UV irradiation + 6 times ethanol (500 ppm) 93 Positive control group UV irradiation + Retinoic acid 10uM 45

As confirmed in Table 2, the cedar extract of Examples 1 to 3 in human normal fibroblast culture experiments significantly inhibited the biosynthesis of MMP-1 produced by ultraviolet B. In addition, when treated with a concentration of 500 ppm of 4-fold ethanol of Example 2 and 500 ppm of 6-fold ethanol of Example 3, MMP-1 biosynthesis was inhibited to a similar degree to the control group not subjected to UV irradiation. Therefore, the cedar extract prepared by the production method of the present invention can be seen that by reducing the biosynthesis of MMP-1, a tissue degrading enzyme in the skin to alleviate the collagen reduction in the skin to improve the skin wrinkles. In addition, the experiments treated with the same concentration as the hydrothermal extract was able to confirm the superior effect of improving the skin wrinkles.

Test Example 3: Determination of DPPH Free Radical Scavenging Activity

100 μl (0.4 mM) of DPPH solution dissolved in ethanol and cedar extracts prepared in Examples 1 to 3 and Comparative Example 1 were added to the 96-hole plate incubator at the concentration of 10 ppm, 100 ppm, and 500 ppm at the same concentration. Reacted. After the reaction, the absorbance was measured by ELISA at 517 nm to measure DPPH radical scavenging activity. BHT was used as a positive control. The results are shown in Table 3 below.

division DPPH free radical scavenging effect (%) Control group 0 Comparative Example 1

Hot Water Extract (10ppm) 3 Hot Water Extract (100ppm) 9 Hot Water Extract (500ppm) 15 Example 1

2-fold ethanol (10 ppm) 4 2-fold ethanol (100 ppm) 12 2-fold ethanol (500 ppm) 18 Example 2

4-fold ethanol (10 ppm) 16 4-fold ethanol (100 ppm) 79 4-fold ethanol (500 ppm) 56 Example 3

6-fold ethanol (10 ppm) 18 6-fold ethanol (100 ppm) 72 6-fold ethanol (500 ppm) 55 Positive control group BHT (100 ppm) 78

As confirmed in the table, the cedar extracts of Examples 1 to 3 in the DPPH free radical scavenging activity experiment increased the antioxidant activity in a concentration-dependent manner. In addition, when treated with a concentration of 100 ppm of 4-fold ethanol of Example 2 and a concentration of 100 ppm of 6-fold ethanol of Example 3, DPPH free radical scavenging activity was similar to that of the BHT control group. Therefore, it can be seen that the cedar extract from which the polysaccharide is removed by the alcohol precipitation method of the present invention exhibits excellent antioxidant activity.

Test Example 4: Determination of SOD (Superoxide Dismutase) -like Activity

50mM phosphate buffer (pH 7.5), 1.5mM NBT, 3mM Xanthine, 0.13 units / ml Xanthine oxidase and cedar extracts of Examples 1 to 3 and Comparative Example 1 were prepared at a concentration of 10 ppm, 100 ppm, and 500 ppm in a 96-hole plate incubator. Was added and reacted at 37 ° C. for 30 minutes. After the reaction, the absorbance was measured by ELISA at 560 nm to determine SOD-like activity. Ascorbic acid (L-ascorbic acid) was used as a positive control. The results are shown in Table 4 below.

division SOD-like activity (%) Comparative Example 1

Hot Water Extract (10ppm) 0 Hot Water Extract (100ppm) 2 Hot Water Extract (500ppm) 5 Example 1

2-fold ethanol (10 ppm) One 2-fold ethanol (100 ppm) 6 2-fold ethanol (500 ppm) 10 Example 2

4-fold ethanol (10 ppm) 11 4-fold ethanol (100 ppm) 27 4-fold ethanol (500 ppm) 49 Example 3

6-fold ethanol (10 ppm) 12 6-fold ethanol (100 ppm) 30 6-fold ethanol (500 ppm) 52 Positive control group L-ascorbic acid (100 ppm) 20

As confirmed in the table, the cedar extracts of Examples 1 to 3 in the SOD-like activity experiment increased the antioxidant activity in a concentration-dependent manner.

Test Example 5: Inhibitory effect of TNF-α (Tumor necrosis factor) and COX-2 (cyclooxygenase-2) induced by LPS (Lipopolysaccharide)

Human normal fibroblasts were cultured in a 12-hole plate incubator at a concentration of 1 X 10 ⁵ and then treated with LPS, and the cedar extracts obtained in Examples 1 to 3 and Comparative Example 1 were included at concentrations of 10 ppm, 100 ppm and 500 ppm. Was replaced with medium. Cells were harvested on day 2 and cultured to quantify the amount of TNF-α and COX-2 induced using Western blot method. The untreated group cultured without the cedar extract was taken as 100 as a control and a comparison with the measured value was calculated. The results are shown in Table 5 below.

division TNF-α biosynthesis (%) COX-2 biosynthesis (%) Control group 100 100 Comparative Example 1

Hot Water Extract (10ppm) 95 100 Hot Water Extract (100ppm) 83 93 Hot Water Extract (500ppm) 66 72 Example 1

2-fold ethanol (10 ppm) 91 95 2-fold ethanol (100 ppm) 79 83 2-fold ethanol (500 ppm) 56 72 Example 2

4-fold ethanol (10 ppm) 55 67 4-fold ethanol (100 ppm) 43 13 4-fold ethanol (500 ppm) 28 2 Example 3

6-fold ethanol (10 ppm) 46 54 6-fold ethanol (100 ppm) 32 20 6-fold ethanol (500 ppm) 26 One

As confirmed in the table, it is confirmed that the cedar extracts of Examples 1 to 3 inhibited the inflammatory response by concentration-dependently reducing the biosynthesis of TNF-α and COX-2 produced by treating LPS which is an inflammatory trigger. It was. In addition, it was confirmed that the cedar extract prepared by the production method of the present invention effectively inhibits the skin inflammatory response as compared to the hot water extract and thereby also has an excellent effect of alleviating skin problems.

Test Example 6: MTT assay

Human normal fibroblasts were cultured in a 96-hole plate incubator at a concentration of 1 X 10 ⁵ and then cultured by replacing the cedar extract obtained in Examples 1 to 3 and Comparative Example 1 with a medium containing 100pm, 500ppm and 1000ppm. It was. After 1 day of culture, the medium was removed and the cells were washed twice with phosphate buffer solution. MTT was dissolved in phosphate buffer solution at a concentration of 3 mg / ml, and diluted with 1/10 of the fresh medium, and then added thereto, followed by incubation for 4 hours. After incubation, all medium was removed, DMSO was dissolved and dissolved, and quantified by ELISA method. The cell viability of the control group not containing the cedar alcohol precipitation method extract was set to 100, and the comparison with each measured value was calculated. Results according to the following formula are shown in Table 6.

Cell survival rate (%) = (blemish of sample added / absorbance of control) × 100

division Cell survival rate (%) Negative control group 100 Comparative Example 1

Hot Water Extract (100ppm) 84 Hot Water Extract (500ppm) 79 Hot Water Extract (1000ppm) 31 Example 1

2-fold ethanol (100 ppm) 85 2-fold ethanol (500 ppm) 80 2-fold ethanol (1000 ppm) 47 Example 2

4-fold ethanol (100 ppm) 91 4-fold ethanol (500 ppm) 85 4-fold ethanol (1000 ppm) 81 Example 3

6-fold ethanol (100 ppm) 92 6-fold ethanol (500 ppm) 89 6-fold ethanol (1000 ppm) 83

As can be seen from the table, in the cell viability measurement experiment, the cedar extract of the example was treated with the same concentration as compared to the hydrothermal extract and confirmed the cell viability, showed a difference of about 10% at 100ppm, 500ppm, but at 1000ppm Cell death was more than 45% under 4 and 6 times ethanol conditions. Therefore, the cedar extract according to the production method of the present invention was confirmed to have a significantly superior cell viability effect in the experiment treated with the same concentration as the hot water extract.

Preparation Example 1 Cream Preparation

A cosmetic containing cedar extract prepared in Example 3 of the present invention was prepared according to the composition of Table 7 below.

ingredient Content (% by weight) Cedar extract 3.0 Pro-type glycerin monostearate 2.0 Stearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Purified vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaruronate 4.0 Preservative, fragrance, pigment a very small amount Purified water Balance Sum 100

Preparation Example 2 Flexible Lotion

A flexible lotion containing the cedar extract of Example 3 in the composition shown in Table 8 below was prepared by a conventional method.

ingredient Content (% by weight) Cedar extract 1.0 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl vinyl polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Preparation Example 3: Emulsion Base

An emulsion base containing cedar extract with the composition shown in Table 9 below was prepared in a conventional manner.

ingredient Content (% by weight) glycerin 3.00 Disodium iodide 0.02 Polyglyceryl-3-methylglucoside distearate 1.50 Cetearyl alcohol 0.50 Caprylic / capric triglyceride 7.00 Polyacrylamide & C13-14 Isoparaffin & Laureth-7 0.60 Cedar extract 2.0 Incense, preservative a very small amount Purified water Balance system 100

Claims (10)

Preparing a cedar crude extract; And adding ethanol to the crude extract to remove inactive polysaccharides. According to claim 1, The cedar extract manufacturing method
(A) adding a solvent of 5 to 10 times the weight to the dried cedar leaves or stems and extracting hot water at 70 ~ 90 ℃;
(B) filtering and recovering the obtained hot water extract, and concentrating under reduced pressure to 1/10 to 1/3 of the initial volume;
(C) adding 2-6 times the volume of ethanol to the final concentrate and stirring until precipitation occurs; And
(D) a method of producing a cedar extract, characterized in that it comprises the step of filtering out the precipitate. The method according to claim 2, wherein in the step (C), 4 to 6 times the volume of ethanol of the final concentrate is added. Cosmetic composition containing 0.001 to 50% by weight of the cedar extract prepared by any one of claims 1 to 3. The composition of claim 4, wherein the composition is a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizer, a nutrition lotion, a massage cream, a nutrition cream, a moisture cream, a hand cream, an essence, a pack, a soap, a shampoo , Cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, milky lotion, press powder, loose powder and eye shadow of the cosmetic composition, characterized in that consisting of one of the formulations selected from the group consisting of. The cosmetic composition according to claim 4, wherein the composition is for anti-aging. The cosmetic composition according to claim 4, wherein the composition is for antioxidant. The cosmetic composition according to claim 4, wherein the composition is for wrinkle improvement. The cosmetic composition according to claim 4, wherein the composition is for anti-inflammatory. The cosmetic composition according to claim 4, wherein the composition is for stimulating alleviation.