KR101926263B1 - Cosmetic compositions containing fermented extract of Origanum majorana, Corchorus olitorius and Melissa officianalis - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed and fermented extract of Origanum majorana, Corchorus olitorius, and Melissa officianalis as an effective component. The mixed and fermented extract of Origanum majorana, Corchorus olitorius, and Melissa officianalis exhibits excellent effects on skin wrinkle reduction, skin whitening, skin moisturizing, skin irritation reduction, and antioxidation.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a cosmetic composition containing a fermented extract of marjoram, molokia and lemon balm,

The present invention relates to a cosmetic composition comprising as an active ingredient a mixed fermentation extract of marjoram, molokia and lemon balm, and more particularly to a cosmetic composition containing margarame, molokia and mixed fermentation extract of lemon balm, .

Skin is the largest organ in the human body, and it is on the outermost layer that surrounds our body, protecting not only the important organs of the body, but also protecting our bodies from external shocks. The skin is constantly in contact with changes in the external environment such as ultraviolet rays, temperature changes, moisture, dust, air pollution, etc. and has additional functions to protect the skin itself from external harm to a certain level.

The skin is divided into the epidermis and the dermis, closely to the subcutaneous fat. The epidermis is a multilayered epithelium with its main component being keratin, and the dermis is a skin layer near the skin, which is an interconnected tissue membrane that is reinforced by a vigorous network of elastic fibers. The subcutaneous layer contains connective tissue that is rooted from the dermis. These connective tissues are complexly intertwined like blood vessels and nerves crossing each other and are closely attached to the skin muscles, so that the muscles are connected to the dermal layer. Thus, each layer of skin plays a unique role and plays an important role in protecting our body. So skin care is a big part of our lives for a long time. However, the increase in allergic substances related to air pollution such as fine dusts and dusts, and modern people's eating habits and living environment are affecting the skin. Accordingly, attempts have been made to protect skin from such harmful environments and to improve skin diseases.

The inventors of the present invention have studied the activity of preparing a mixed extract of marjoram, molokia and lemon balm while studying natural products having skin-improving effects such as antioxidant and anti-inflammation. At this time, the marzolam, molokia and lemon balm mixed fermentation extract And it can be used effectively as a cosmetic material. Thus, the present invention has been completed.

Korean Patent Publication No. 2003-0065095 (June 08, 2003) Korean Patent Publication No. 10-2013-0085012 (Feb.

The object of the present invention is to provide a cosmetic composition containing marjoram, molokia, and lemon balm mixed fermented extract as an active ingredient, showing excellent skin improving effect.

To achieve the above object, according to the present invention, there is provided a skin-improving cosmetic composition comprising marjoram, molokia, and lemon balm mixed fermentation extract. At this time, the margarame, molokia and lemon balm mixed fermentation extract as active ingredients are contained in an amount of 0.01 to 90% by weight based on the total weight of the cosmetic composition.

In addition, the mixed fermentation extract is obtained by mixing marzolam, molokia and lemon balm at a weight ratio of 1: 2: 1 to 2: 1: 2, more preferably 2: 1: 2 And extracted.

The fermentation extract may be a yeast fermentation extract, a lactic acid fermentation extract or a fermentation extract, and more preferably a fermentation extract.

At this time, the fermentation is carried out by at least one vinegar selected from the group consisting of fruit candies such as apple vinegar, persimmon vinegar or grape vinegar, and cereals such as malt vinegar or brown rice vinegar.

The cosmetic composition for skin improvement containing the margarame, molokia and mixed lemon balm fermentation extract can be used for wrinkle improvement, skin whitening, skin moisturizing, skin irritation relief or antioxidant.

The mixed fermentation extract of marjoram, molokia and lemon balm according to the present invention is excellent in skin wrinkle, skin whitening, skin moisturizing, skin irritation alleviation and antioxidant effect, and can be usefully used as a cosmetic for improving skin.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition comprising as an active ingredient an extract of margarame, molokia and lemon balm mixed fermentation.

The Origanum majorana used in the present invention is believed to be planted in the highest mountain so that Venus, the goddess of love and beauty, can produce it in the sea and receive plenty of sunlight, and its excellent fragrance is said to have come from Venus's hand. Aphrodite's most important herb is marjoram, which is expressed as a symbol of beauty. It is used as a lotion, a bathing agent, and has a cleansing, soothing and sterilizing action on the skin.

Corchorus olitorius is called "the king's vegetable" because it is strong in vitality and strong against pests and diseases. The story that the ancient Egyptian royal family enjoyed eating to regenerate has been derived and is still called the mystic herb. Molochia contains beta carotene, which is good for the detoxification of the body, and polyphenol which is an antioxidant. It is effective for moisturizing and skin troubles.

The lemon balm (Melissa officianalis) is called Melissa, which means the Malay penis from Greek. Melissa honey is a name given to bees like Melissa's honey. Melissa's honey is also used for wound healing and inflammatory digestive diseases. Lemon balm is effective in improving blood circulation and mood, and it is often used in inflammatory skin diseases due to the inflammatory effect of lemon balm oil.

According to the present invention, there is provided a skin improving cosmetic composition comprising, as an active ingredient, a fermentation extract of margarame, molokia and lemon balm. The mixed extracts of marjoram, molokia and lemon balm showed excellent skin improving activity, but the mixed fermentation extracts thereof showed more excellent skin improving activity.

The fermented extract is prepared by mixing marjoram, molokia, and lemon balm, or by fermenting at the same time as extraction. As the fermentation strain, at least one selected from the group consisting of yeast, lactic acid bacteria, and acetic acid bacteria can be used. According to one embodiment of the present invention, Saccharomyces cerevisiae is used as the yeast, and Lactobacillus plantarum is used as the lactic acid bacteria.

More preferably, the fermented extract is a mixed fermented extract of marjoram, molokia and lemon balm. Compared with other fermented extracts, the mixed extract of marjoram, molokia and lemon balm showed better skin wrinkles, skin whitening, skin moisturizing, skin irritation alleviation and antioxidant activity.

Vinegar has long been a favorite of both East and West. In China, there was already vinegar in Confucius period, and in Korea, it was known that the method of making vinegar was introduced in China during the Three Kingdoms period. It is the oldest sweetener in humans, and vinegars have been made in all parts of the world for many years. Depending on the local climate and the variety of crops, the vinegar variety also varies. Prevention and immunity enhancement. In the present invention, the vinegar was used for preparing a mixed fermented extract of Marjoram, Molokia and Lemon balm.

According to one embodiment of the present invention, at least one kind of vinegar selected from the group consisting of fruit candles such as apple vinegar, persimmon vinegar, grape vinegar, and cereal grains such as malt vinegar and brown rice vinegar is used for the ultra-fermentation.

In this case, the margarame, molokia and lemon balm mixed fermentation extract as an active ingredient is contained in an amount of 0.01 to 90% by weight, more preferably 0.1 to 20% by weight based on the total weight of the cosmetic composition.

According to one embodiment of the present invention, when preparing the mixed fermentation extract, margarol, molokia and lemon balm are mixed and extracted at a weight ratio of 1: 2: 1 to 2: 1: 2, respectively. 2: 1: 2, it is more preferable because it shows the highest yield and excellent skin improving activity.

The thus-prepared margarame, molokia, and lemon balm mixed fermented extracts exhibited a superior effect of increasing the expression of wrinkle-related factors (Test Example 1), a reduction in the expression of whitening-related factors (Test Example 2), an effect of increasing moisturizing factor expression (Test Example 3), an inflammatory mediator expression inhibitory effect (Test Example 4), an antioxidant effect (Test Example 5) and a skin irritation alleviating effect (Test Example 6).

The cosmetic composition containing the margarame, molokia, and lemon balm mixed fermentation extract of the present invention can be prepared into any of the commonly used formulations, and examples thereof include lotion, cream, essence, cleansing foam, cleansing water, Lotion, body oil, body gel, shampoo, rinse, hair conditioner, hair gel, foundation, lipstick, mascara, makeup base, mask sheet, sun product, soap and the like.

[Example]

Hereinafter, the present invention will be described in detail by way of the following examples and test examples, but the present invention is not limited to these examples.

Example  One: Marjoram , Molokia  And Lemon balm  mix Second fermentation  Extract preparation

900 g of purified water containing 1% of brown rice vinegar (Ottogi, total acidity of 6-7%) was added to a sample of 3 g of dried marjoram, 3 g of molokia and 3 g of lemon balm (dry weight ratio = 1: And immersed in the solution for one day at room temperature. Thereafter, filtration was performed using a filter paper (Qualitative Filter Papers No. 2), and the filtrate was obtained.

Example  2 to 7: Marjoram , Molokia  And Lemon balm  mix Second fermentation  Extract preparation

The weight ratio of dried marjoram, molokia and lemon balm was adjusted as shown in Table 1 and extracted and filtered in the same manner as in Example 1.

division Mixing ratio Yield (%) Example 2 Marjoram: Molokia: lemon balm = 1: 1: 2 68 Example 3 Marjoram: Molokia: lemon balm = 1: 2: 1 72 Example 4 Marjoram: Molokia: lemon balm = 1: 2: 2 80 Example 5 Marjoram: Molokia: lemon balm = 2: 1: 1 78 Example 6 Marjoram: Molokia: lemon balm = 2: 1: 2 95 Example 7 Marjoram: Molokia: lemon balm = 2: 2: 1 88

Example  8: Marjoram , Molokia  And Lemon balm  Preparation of mixed yeast fermented extract

Dried marjoram, molokia and lemon balm were mixed in a weight ratio of 2: 1: 2 (9 g) and mixed with 1500 g of a mixed solvent of 1,3-butylene glycol and purified water in a ratio of 3: 7 (w / w). The mixture was immersed for 3 days and extracted with an extractor equipped with a cooling condenser at 100 DEG C for 8 hours. Saccharomyces cerevisiae was inoculated into the extract, fermented at 25 ° C for 3 days, sterilized at 95 ° C for 1 hour, filtered through a filter paper (Qualitative Filter Papers No. 2), and filtrated.

Example  9: Marjoram , Molokia  And Lemon balm  mix Lactobacillus  Fermented extract preparation

Dried marjoram, molokia and lemon balm were mixed in a weight ratio of 2: 1: 2 (9 g) and mixed with 1500 g of a mixed solvent of 1,3-butylene glycol and purified water in a ratio of 3: 7 (w / w). The mixture was immersed for 3 days and extracted with an extractor equipped with a cooling condenser at 100 DEG C for 8 hours. The extracts were inoculated with Lactobacillus plantarum, fermented at 25 ° C for 3 days, sterilized at 95 ° C for 1 hour, filtered through a filter paper (Qualitative Filter Papers No. 2), and filtered.

Comparative Example  One: Marjoram , Molokia  And Lemon balm  mix Heat number  Extract preparation

Dried marjoram, molokia and lemon balm were mixed in a weight ratio of 2: 1: 2 (9 g) and mixed with 1500 g of a mixed solvent of 1,3-butylene glycol and purified water in a ratio of 3: 7 (w / w). The mixture was immersed for 3 days and boiled for 8 hours at 100 ° C in an extractor equipped with a cooling condenser. The mixture was filtered using a filter paper (Qualitative Filter Papers No. 2), and then filtrated.

Comparative Example  2: Marjoram Heat number  Extract preparation

9 g of dried marjoram was mixed with 1500 g of a mixed solvent of 1,3-butylene glycol and purified water in a ratio of 3: 7 (w / w). The mixture was immersed for 3 days and boiled for 8 hours at 100 ° C in an extractor equipped with a cooling condenser. The mixture was filtered using a filter paper (Qualitative Filter Papers No. 2), and then filtrated.

Comparative Example  3: Molokia Heat number  Extract preparation

9 g of dried molochia were mixed with 1500 g of a mixed solvent of 1,3-butylene glycol and purified water in a ratio of 3: 7 (w / w). The mixture was immersed for 3 days and boiled for 8 hours at 100 ° C in an extractor equipped with a cooling condenser. The mixture was filtered using a filter paper (Qualitative Filter Papers No. 2), and then filtrated.

Comparative Example  4: Lemon balm Heat number  Extract preparation

9 g of dried lemon balm was mixed with 1500 g of a mixed solvent of 1,3-butylene glycol and purified water in a ratio of 3: 7 (w / w). The mixture was immersed for 3 days and boiled for 8 hours at 100 ° C in an extractor equipped with a cooling condenser. The mixture was filtered using a filter paper (Qualitative Filter Papers No. 2), and then filtrated.

Test Example  One: Wrinkle-related factor  Confirmation of increased expression

Fibroblast (HDFa) was adjusted to 5 × 10 ⁶ cells / well using IMDM medium supplemented with 10% FBS, and then inoculated on a 6-well plate and cultured for 24 hours. After culturing, the samples of the above-mentioned Examples and Comparative Examples were treated and cultured for 48 hours. RNA was extracted from the cultured cells and the increase rate of collagen expression was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The increase rate of collagen expression was calculated by the following formula, and the results are shown in Table 2 below. As a control group, adenosine was used.

Collagen expression rate (%) = (sample collagen expression level / control collagen expression level) × 100

Name of sample Treatment concentration (w / v%) Collagen expression rate (%) Example 6 0.5 92.4 Example 8 0.5 83.6 Example 9 0.5 84.9 Comparative Example 1 0.5 81.5 Comparative Example 2 0.5 84.5 Comparative Example 3 0.5 80.2 Comparative Example 4 0.5 80.7 Adenosine 0.01 83.8

As shown in the above Table 2, the fermented extract of the example of the present invention at the same concentration treatment was equal to or higher than that of the control group, and exhibited a higher collagen expression ratio than the comparative example. In particular, the highest extract of collagen was obtained from the fermented extract of Example 6.

Test Example  2: skin whitening Related Parameters  Confirmation of expression inhibition

B16F10 melanoma cells were adjusted to 1 × 10 ⁶ cells / well using DMEM supplemented with 10% FBS, and then inoculated on a 6-well plate and cultured for 18 hours. After the cultivation, the cells were exchanged with DMEM medium treated with? -MSH, and the samples of the above-mentioned Examples and Comparative Examples were treated and cultured for 48 hours. RNA was extracted from the cultured cells and the inhibitory effect of tyrosinase was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The expression rate of tyrosinase was calculated by the following formula, and the results are shown in Table 3 below. Arbutin was used as a control.

Tyrosinase expression rate (%) = (amount of tyrosinase expression in the sample / expression amount of tyrosinase in the control) × 100

Name of sample Treatment concentration (w / v%) Tyrosinase expression rate (%) Example 6 0.5 50.8 Example 8 0.5 66.4 Example 9 0.5 60.2 Comparative Example 1 0.5 62.8 Comparative Example 2 0.5 62.3 Comparative Example 3 0.5 63.4 Comparative Example 4 0.5 64.8 Arbutin 0.01 56.4

As shown in Table 3, the fermented extract of the example of the present invention showed a superior effect of decreasing the expression of tyrosinase when treated at the same concentration, and the fermented extract of marjoram, molokia and lemon balm of Example 6, Effect.

Test Example  3: Confirmation of increased expression of skin moisturizing factor

Fibroblast (HDFa) was adjusted to 1 × 10 ⁶ cells / well using IMDM medium supplemented with 10% FBS, and then inoculated on a 6-well plate and cultured for 24 hours. After incubation, the samples were treated and cultured for 48 hours. RNA was extracted from the cultured cells and the expression of Hyaluronan Synthase-2 (HAS-2) was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The HAS-2 expression increase rate was calculated by the following equation, and the results are shown in Table 4 below. As a control group, hyaluronic acid was used.

HAS-2 expression ratio (%) = (expression amount of sample HAS-2 / expression amount of control HAS-2) × 100

Name of sample Treatment concentration (w / v%) HAS-2 expression rate (%) Example 6 0.5 90.3 Example 8 0.5 88.3 Example 9 0.5 85.7 Comparative Example 1 0.5 84.5 Comparative Example 2 0.5 82.6 Comparative Example 3 0.5 80.6 Comparative Example 4 0.5 80.2 Hyaluronic acid 0.01 86.3

As shown in Table 4, the mixed fermented extract of the examples of the present invention showed an excellent effect of increasing the moisturizing factor expression compared to the extract of the comparative example at the same concentration treatment.

Test Example  4: Confirming inhibition of skin inflammatory factor expression

HaCaT cells were cultured in DMEM medium supplemented with 10% FBS at 1 × 10 ⁶ cells / well, inoculated on a 6-well plate and cultured for 24 hours. After culturing, the cells were irradiated with UVB, treated with the sample, and cultured for 48 hours. RNA was extracted from the cultured cells and the effect of inhibiting the expression of TNF-α was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The expression rate of TNF-α was calculated by the following equation, and the results are shown in Table 5 below. Dexamethasone was used as a control.

TNF-α expression ratio (%) = (expression amount of sample TNF-α / expression amount of control group TNF-α) × 100

Name of sample Treatment concentration (w / v%) TNF-α expression rate (%) Example 6 0.5 51.2 Example 8 0.5 62.3 Example 9 0.5 58.7 Comparative Example 1 0.5 60.8 Comparative Example 2 0.5 62.5 Comparative Example 3 0.5 63.7 Comparative Example 4 0.5 64.5 Dexamethasone 0.01 52.4

As shown in Table 5, the fermented extract of the example of the present invention exhibited a superior effect of reducing the expression of inflammatory factors during the same concentration treatment, and in particular, the margarame, molokia and lemon balm superfine fermentation extracts of Example 6, Even more excellent results were obtained.

Test Example  5: Confirmation of antioxidant effect

Fibroblast (CCD-986Sk) was adjusted to 1 × 10 ⁶ cells / well using IMDM medium supplemented with 10% FBS, and then inoculated on a 6-well plate and cultured for 18 hours. After incubation, the samples were treated and cultured for 48 hours. RNA was extracted from cultured cells and the expression of superoxide dismutase-2 (SOD-2) was detected by reverse transcription polymerase chain reaction (RT-PCR). The increase rate of SOD-2 expression was calculated by the following formula, and the results are shown in Table 6 below. Vitamin C was used as a control.

SOD-2 expression ratio (%) = (SOD-2 expression level / control SOD-2 expression level) × 100

Name of sample Treatment concentration (w / v%) SOD-2 expression rate (%) Example 6 0.5 89.7 Example 8 0.5 82.5 Example 9 0.5 83.9 Comparative Example 1 0.5 80.6 Comparative Example 2 0.5 80.0 Comparative Example 3 0.5 79.5 Comparative Example 4 0.5 79.3 Vitamin C 0.01 86.7

As shown in Table 6 above, the fermentation extract of the example of the present invention exhibited superior antioxidative effect to the extract of the comparative example at the same concentration treatment.

Example  10: Marjoram , Molokia  And Lemon balm  mix Second fermentation  Preparation of formulations using extracts

A lotion containing marjoram, molokia and lemon balm mixed fermentation extract was prepared according to the conventional method with the composition and content of Table 7 below.

(weight%) Control Example Example 10 Mixed fermented extract (Example 6) - 2 glycerin 8 8 Butylene glycol 4 4 Caprylic / capric triglyceride 10 10 Cetearyl glucoside 1.5 1.5 Sorbitan stearate 0.4 0.4 Cetearyl alcohol One One Phenoxyethanol 0.3 0.3 Ethylhexyl Glycerin 0.05 0.05 incense 0.15 0.15 Purified water To 100 To 100

Test Example  6: Confirmation of skin irritation mitigation effect

Ten people in their 20s and 30s who do not have skin allergic symptoms were subjected to skin irritation through a tape stripping method on the inferior part of the lower arm, and then the lotion of Example 10 and the control lot was applied to induce skin irritation The degree of sedation was calibrated relative to each other. First, taping was repeated 30 times with a scotch tape on both sides of the underbody to cause skin erythema. After erythema induction, the lotion of Example 10 containing the margarame, molokia and lemon balm mixture premix fermentation extract according to the present invention was applied and the lotion of the control example without the complex according to the present invention was applied. The degree of skin irritation was evaluated 3 hours after application.

≪ Evaluation of degree of skin irritation (erythema) >

0 (no response) 1 (very mild erythema) 2 (faint or mild erythema)

3 (clear erythema but weak erythema) 4 (pronounced erythema) 5 (severe erythema)

Name of sample Degree of skin irritation Left Right Control Example One One Example 10 0 0

As shown in Table 8 above, the lotion containing the present invention marjoram, molokia, and lemon balm combined ultra-fermented extract had excellent skin irritation mitigation effect.

Claims (10)

The mixture of margarame, molokia and lemon balm was mixed with each other at a weight ratio of 1: 2: 1 to 2: 1 to 2, followed by fermentation with marjoram, molokia and lemon balm, 0.01 to 90% by weight based on the weight of the cosmetic composition. delete delete delete The cosmetic composition for skin improvement according to claim 1, wherein the fermentation is performed using at least one vinegar selected from the group consisting of apple vinegar, persimmon vinegar, grape vinegar, malt vinegar and brown rice vinegar. The cosmetic composition for improving skin according to claim 1, wherein the cosmetic composition is for improving skin wrinkles. The cosmetic composition for skin improvement according to claim 1, wherein the cosmetic composition is for skin whitening. The cosmetic composition for skin improvement according to claim 1, wherein the cosmetic composition is for skin moisturizing. The cosmetic composition for skin improvement according to claim 1, wherein the cosmetic composition is for alleviating skin irritation. The cosmetic composition for skin improvement according to claim 1, wherein the cosmetic composition is for antioxidation.