KR20180031119A - Natural antimicrobial composition and cosmetic composition containing the same - Google Patents

Abstract

The present invention relates to an antimicrobial composition comprising a natural plant extract, which is free from toxicity, exhibits an excellent antimicrobial effect, can be a substitute for conventional synthetic preservatives, and comprises cavacrol and chebulinic acid as active ingredients. The present invention also relates to a cosmetic composition containing the antimicrobial composition.

Description

Natural antimicrobial composition and cosmetic composition containing same

The present invention relates to a natural antimicrobial composition and a cosmetic composition containing the antimicrobial composition. More particularly, the present invention relates to an antimicrobial composition comprising carbacol and carburonic acid as an active ingredient and a cosmetic composition containing the same.

Conventional cosmetic compositions contain various components including water, and are likely to be contaminated by microorganisms such as bacteria or fungi. The negative aspects of contamination of cosmetic compositions can be summarized in two ways: the first is the risk to the consumer's health, and the second is the decline in the value of the product. Most cosmetic compositions are applied to the skin. Therefore, when a product contaminated with microorganisms is applied to skin, inflammation or skin trouble may occur due to microorganisms introduced through the wound. In particular, contamination with pathogenic microorganisms can cause serious health problems. Other products such as fungi and germs that are propagated cause changes in the product properties due to destruction of emulsion and emulsification, changes in pH of the product, and result in a drop in the value of the product. For this reason, the cosmetic composition should have a suitable level of preservative system depending on the characteristics of the formulation. Preservatives that can be used in cosmetic compositions vary from country to country. In Korea, the type of preservative and the compositional limit that can be used in cosmetics are specified by the Korea Food & Drug Administration (Cosmetic Act, Articles 4, 3, 9 and Article 13 (6), Korea Food & Drug Administration Notice 2000-27).

Preservatives used in conventional cosmetic compositions generally include chemical preservatives (synthetic preservatives) which are easy to manufacture, cheap in cost, and easy to mass-produce. Examples of such preservatives include Metyl Paraben, Ethyl Paraben, Propyl paraben, butyl paraben and the like and quaternium-15, imidazolidinyl urea diazolidinyl urea, phenoxyethanol, Benzalkonium Chloride, chlorohexidine gluconic acid and Trichlosan, and the like. However, such a chemical preservative inhibits the growth of microorganisms, thereby imparting stability to the cosmetic composition, but at the same time, it may cause irritation to the user's skin. Typical examples are formalin-free preservatives, including diazolidinyl urea and imidazolidinyl urea. These have recently been reviewed for safety worldwide and their use is on the decline and their use is limited. In addition, parabens such as Metyl Paraben, Ethyl Paraben, Propyl Paraben and Butyl Paraben, which are most safe as conventional chemical preservatives and commonly used in cosmetics and pharmaceuticals, Even preservatives are disbelief in the use of the criteria as permitted by skin allergies, the possibility of environmental hormones and the generation of resistant bacteria. Accordingly, there is a need for a natural preservative that can reduce the content of the preservative in the cosmetic composition as much as possible and replace the chemical preservative such as not using a synthetic preservative.

The requirements of these natural preservatives should be able to prevent hypoallergenic, low toxicity and resistance to the skin, as well as antimicrobial activity against microorganisms such as bacteria and fungi. However, some raw materials of natural preservatives known to date are too dark and have a strong raw material pick-up. Therefore, they are limited or unsuitable for use in cosmetics requiring a good quality product, or they lower the viscosity of cosmetics and affect the emulsifying system. It was difficult to use. In addition, it has a narrow antimicrobial spectrum as compared with a chemical preservative, and its physicochemical stability against heat, light, and oxygen is very low, so that the antimicrobial active ingredient is decomposed during the distribution period, The problem is that most of them are not commercialized due to limitations as natural preservatives.

The inventors of the present invention have conducted intensive studies on natural plant extracts having antimicrobial activity and found that when carbacol and carblinic acid are mixed as an active ingredient, they exhibit a synergistic effect on the inhibition of the growth of contrast microorganisms when they are used alone And to provide an antibacterial composition containing the same as an active ingredient and a cosmetic composition containing the antibacterial composition.

An object of the present invention is to provide an antimicrobial composition comprising a natural plant extract excellent in antimicrobial activity against microorganisms.

It is an object of the present invention to provide a natural preservative containing said antimicrobial composition.

It is also an object of the present invention to provide a cosmetic composition and an aqueous dispersion containing the antimicrobial composition as a preservative component.

The present invention provides an antimicrobial composition comprising carbacol and a carburonic acid as an active ingredient.

The antimicrobial composition according to one embodiment of the present invention contains an oregano extract containing carbacol and a carburonic acid as an active ingredient.

In addition, the antimicrobial composition according to an embodiment of the present invention contains an oregano extract containing carbacol and a Ghazu extract comprising the above carbolinic acid as an active ingredient.

The antimicrobial composition according to an embodiment of the present invention may further include one or more extracts selected from the group consisting of Lilium extract, green tea extract, orchid extract, strawberry herb extract, Parsley extract, and the like, but is not limited thereto.

The antimicrobial composition according to an embodiment of the present invention has an antibacterial activity against microorganisms selected from bacteria and fungi. When a mixture of carbacol and kebulic acid is used, in particular, bacteria such as Pseudomonas aeruginosa and Candida albicans Candida albicans, Aspergillus niger, and the like.

The present invention also provides a cosmetic composition comprising the antimicrobial composition as a preservative component and a natural preservative.

In addition, the present invention provides an aqueous dispersion containing the above-mentioned antimicrobial composition. At this time, the aqueous dispersion according to an embodiment of the present invention is characterized by being a transparent aqueous dispersion having a visible light transmittance of 90% or more at 400 to 700 nm light ray at a concentration containing 10% by weight of the antimicrobial composition.

The antimicrobial composition according to the present invention has no toxicity to the human body and exhibits an excellent antibacterial effect and is expected to be useful as a natural preservative ingredient in cosmetics, foods, and quasi-drugs by replacing conventional synthetic preservatives.

1 shows the result of skin irritation test of the antimicrobial composition prepared in Example 1. Fig.

The antimicrobial composition comprising the natural plant extract according to the present invention will be described in detail below. Unless otherwise defined in the technical terms and scientific terms used herein, the person skilled in the art will understand In the following description, well-known functions and constructions that may unnecessarily obscure the gist of the present invention will not be described.

The official name of "oregano" in the present invention is Origanum vulgare L., the most common species of Lamiaceae flowering Origanum. Oregano is a perennial herb that is native to the western and southwestern parts of Eurasia and the Mediterranean region. It is a plant that grows up to 20-80 cm and has a 1-4 cm long camellia. Thus, the oregano extract in the present invention may be origanum vulgare extract using oregano leaves and stem, but is not limited thereto. In addition, the oregano extract according to the present invention contains carvacrol as a main indicator substance (containing 90 wt% or more), and the other ingredients include sabinene, 1,8-cineole terpineol,? -terpinene,? -terpinene, caryophyllene, geracrene D, thymol (? -terpinene),? -terpinene, , p-cimene, myrcene, spathulenol, and the like, but the present invention is not limited thereto.

In the present invention, "Gaza" is a fruit of Gaza (Terminalia chebula Retz.) Or Terminalia chebula Retz. Var. Tomentella Kurt. The flowering period is from June to August and the fertilization period is 8 It is from October to October, and in the fall, mature fruit is picked and dried in the sun. The fruit-like phase of dried Gaza is long egg-shaped or egg-shaped and has a length of 2.5 cm to 3.0 cm and a diameter of 1.5 cm to 2.5 cm. The outer surface is yellowish brown to brownish in color, with five prominent wrinkles vertically, with irregular wrinkles therebetween, and there is a scar on the base. The vagina is hard, the side cut off is dark brown, and the thickness of the flesh is 2mm to 5mm. The periplasm is about 5 mm thick, yellowish brown, the quality is very hard, there is a brown suture line, and there is one seed of about 5 mm in diameter at the center. Let go have a slight peculiar smell, taste a little bitterly to wear. Thus, the Ghazi extract in the present invention may be a terminalia chebula fruit extract, but the present invention is not limited thereto. Also, the indicator substances of the Ghassa extract according to the present invention include chebulic acid, chebulagic acid, chebulinic acid, corilagin, punicalin, But are not limited to, Punicalagin and Methyl neochebulagate.

In the present invention, the term " glutinous "is a perennial herb belonging to Magnoliaceae, including Magnolia obovata Thunberg, Magnolia officinale Rehder et Wils and Machilus thunbergii Sieb. Et Zucc, . Thus, the extract of the present invention may be Magnolia obovata bark extract, but not limited thereto.

The term "green tea" in the present invention has not only been consumed as a symbolic tea since BC, but recently, the pharmacological mechanism of the various components contained in green tea has gradually become known, and thus the value of the plant has been recognized. Camellia sinensis It is extracted from the leaves and leaves of Camellia Sininsis by treatment with oxidative enzymes present in the leaves of the tea leaves with pyrogen or steam. Accordingly, the green tea extract of the present invention may be Camellia sinensis leaf extract, but is not limited thereto.

The term "orchid" in the present invention is a perennial herbaceous plant belonging to the genus Orchidaceae. Specifically, the term " orchidaceae " refers to a plant belonging to the genus Acanthopipium Blume, Acianthus, Aerides, But are not limited to, Angraecum, Amitostigma, Anoectochilus, Ansellia, Aplectrum, Arethusa, Arundina, Arundina, Blume, Ascocentrum, Brassia, Beltilla, Bulbophyllum, Calanthe, Calypso, Cattleya, Cattleya, And are classified into three genera: Cephalanthera, Coelogyne, Coeloglossum, Corallorhiza, Cremastra, Cymbidium, (Cypripedium), Crytopodium, Dendrobium, Elle kies (El eorchis, Epidendrum, Epipogium, Eria, Eulophia, Galeola, Goodyera, and Geodolum. Geodorum, Gymnadenia, Habenaria, Hancockia, Herminium, Kitigorchis, Linaria, Lepariaceae, Listeria, Lycaste, Macodes, Malaxis Soland., Microstylis, Neofinetia, and the like. , Neottia, Oncidium, Oreorchis, Phalaenopsis, Phagmopedium, Pholidota, Plataneta, and the like. Platanthera, Pogonia, Rhynchostylis, Sarcanthus, Spiranthes, Spa sproutis Bloom (sp.), Refers to an orchid plant belonging to the genus such as Spathoglottis Blume, Tipularia, Tainia, Vanda, Vexillabium, Zygopetalum and the like . Accordingly, the orchid extract of the present invention may be Ndrobium Nobile extract extracted from leaves of Ndrobium Nobile, but is not limited thereto.

The term "straw sandalwood" in the present invention belongs to Rosaceae as a perennial perennial herbaceous plant, and it is known that 10 species are distributed in the temperate zone of the Northern Hemisphere and Brazil and South America. In Korea, it grows frequently in the roadside of the mountains and fields, and it is also called Seonghakcho, Yonga, Yongaeko, Hwanghaeko, Hwangyongjang, Gijunseok. Its height grows about 15 ~ 60 cm. It has white hairs all over, with a leaflet shape or a long elliptical leaflet staggered. Yellow flowers bloom in June ~ August. Thus, the extract of the present invention may be Agrimonia Pilosa Extract using leaves of straws, but the present invention is not limited thereto.

In addition, in the present invention, "parsley" is a ladybug and an evergreen herbaceous shrub, and a stem of 30-80 cm high from the tropics is branched from the base. Leaves are alternate and wide elliptical with wavy sawtooth on edge. Accordingly, the Pachiri extract in the present invention may be Pachostemon Cablin leaf extract, but is not limited thereto.

In addition, the extract according to the present invention is extracted according to a conventional method known in the art by adding an extraction solvent to at least one selected from plant leaves, flowers, stems, branches, roots, outposts, bark and fruits. That is, it may be extracted by methods such as cold extraction, ultrasonic extraction, reflux cooling extraction, hot water extraction, room temperature extraction, warm-up extraction, supercritical extraction, etc. using a conventional solvent under ordinary temperature and pressure conditions, It is not. The extraction solvent may be, but is not limited to, water, anhydrous or lower alcohol having 1 to 7 carbon atoms, hexane, chloroform, ethyl ether, acetone, ethyl acetate, butyl acetate, 1,3-butylene glycol, Propylene glycol, and 1,3-propylene glycol. Of these, lower alcohols and water selected from methanol, ethanol, propanol and butanol are preferable. In addition, the extract extracted with the above-mentioned extraction solvent includes all the extracts, fractions and purified products obtained in each step of fractionation or purification, their diluted solution, concentrated solution or dried product, and if necessary, they are subjected to freeze-drying, vacuum drying , Dried by hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying, and the like, and further pulverizing the dried extract. Accordingly, the extract is characterized by having a liquid phase, a concentrated solid phase, and a lyophilized powder phase, as the extract has additional steps such as drying and grinding according to the case after filtration.

The term "antibacterial" in the present invention means an ability to resist bacteria and means all the mechanisms for defending against the action of microorganisms such as bacteria or fungi. Herein, the term "bacterium " refers to an organism belonging to bacteria, which is the most prolific species among living organisms, and may be a pathogenic bacterium. The term" fungal "refers to at least 72,000 species including fungi, yeast, And microorganisms belonging to the same genus.

The present invention provides an antimicrobial composition comprising carbacrol and cerulonic acid as an active ingredient. When each of the carbacol and the carburonic acid is used as a single component, the antibacterial activity against bacteria and fungi is generally low. Particularly, in the case of a single component of the carbulinic acid, it has been found that the antimicrobial activity against bacteria and / or fungi is remarkably low, but when it is mixed with carbacol, the antimicrobial activity shows a synergistic effect.

Preferred examples of the present invention include carbacrol and couulinic acid; Oregano extracts and carbulinic acid containing carbacol; GABA extract comprising carbacol and the above carbolinic acid; An oregano extract containing carbacol, and a ghazu extract containing the above carbolinic acid as an active ingredient, but the present invention is not limited thereto. In addition, in the antimicrobial composition according to an embodiment of the present invention, the carbacol and / or carbulinic acid are not limited to the above-mentioned oregano extract and Ghazale extract if they are plant extracts containing them as main components.

In the antimicrobial composition according to an embodiment of the present invention, the oregano extract containing carbacol may contain 5 to 40% by weight of carbacol based on the whole content of the extract, In the case of the extract, it is possible to contain 10 to 50% by weight of carburonic acid based on the total content.

The antimicrobial composition according to an embodiment of the present invention can have excellent antibacterial activity against bacteria and fungi with the above-mentioned combination, and in particular, it is possible to produce Staphylococcus aureus, E. coli and Pseudomonas aeruginosa, and fungi such as Candida albicans and Aspergillus niger, and exhibit an excellent synergistic effect against the antimicrobial activity used as a single component in the antimicrobial activity against fungi such as Candida albicans and Aspergillus niger.

The carbacol and / or oregano extract according to an embodiment of the present invention has a liquid oily form and exhibits a low solubility in water. However, the solubility in water is lowered by the combined use of the carbulinic acid and / or GAZA extract Can be dramatically increased. These effects are expected to result from changes in the physical properties of the resulting carbolinic acid and / or GAZA extract, which act as a solubilizing agent for water of carbacol and / or oregano extract. In particular, in the case of the above-mentioned GAZA extract, it is considered that the tannin component and the like help improve the solubilization ability of carbacol and / or oregano extract. Therefore, the present invention is not limited thereto.

The antimicrobial composition according to the present invention may further comprise various extracts which can give synergy to the main effect within a range not impairing the intended main effect of the present invention. At this time, a preferred example of the extract may be at least one extract selected from the group consisting of the extract of Leek extract, green tea extract, orchid extract, strawberry seed extract, and Parsley extract, but is not limited thereto.

In addition, the antimicrobial composition according to one embodiment of the present invention may be used by diluting it with at least one solvent selected from glycerin, propanediol, butanediol, hexanediol, and octanediol using carbacol and kebulic acid as an active ingredient, May be at least one selected from glycerin, 1,2-propanediol, 1,3-propanediol, 1,3-butanediol, 1,4-butanediol, 1,6-hexanediol, octanediol and methylpropanediol, It is not.

The antimicrobial composition according to the present invention has no adverse effects on the skin, can provide excellent preservation power for a long period of time, and exhibits excellent antimicrobial activity against bacteria and fungi. The bacteria and fungi include, but are not limited to, Escherichia coli, Pseudomonas aeruginosa, Staphlyococcus aureus, Candida albicans, Aspergillus niger, niger, Bacillus megaterium, and Exophiala moniliae. Examples of the microorganism include bacteria such as Pseudomonas aeruginosa, Candida albicans, Aspergillus oryzae, And exhibits remarkable antimicrobial activity against fungi such as Aspergillus niger.

The minimum inhibitory concentration (MIC, v / v) according to the present invention means a minimum concentration which inhibits the growth of microorganisms by an antimicrobial substance or the like. The antimicrobial composition according to the present invention has a minimum development inhibitory concentration (MIC) of 0.1 to 100 μl / ml (0.01 to 10.0% v / v) for the microorganism. In particular, the antimicrobial composition according to the present invention exhibits a minimum development inhibition concentration of Candida albicans at a concentration of 2 μl / ml (MIC value, 0.2% v / v) or less, and Aspergillus niger, (MIC value, 0.1% v / v) to a minimum development inhibitory concentration of 1 μl / ml. In the minimum development inhibition concentration, 1.0 占 퐇 / ml may be equivalent to 0.1% v / v.

The antimicrobial composition according to an embodiment of the present invention may preferably be a mixture of carbacol and cerulonic acid in a weight ratio of 1: 0.1 to 1: 1, as an active ingredient in the solvent, And they can be mixed and used at the same weight ratio. The antimicrobial composition may be a mixture of 0.1 to 10% by weight of the active ingredient with respect to the total weight of the solvent, and this is prior to the present invention.

The antimicrobial composition according to the present invention may further comprise one or more extracts selected from the group consisting of an extract of Zygomycetum extract, green tea extract, orchid extract, Zygomycethea herb extract, Parsley extract and the like for further antibacterial activity and excellent stability according to the use conditions Of course. At this time, each of the extracts may be used in an amount of 0.1 to 15% by weight based on the total weight of the antimicrobial composition. In addition, the above stability may be not only stability for long-term storage but also stability not causing changes in product properties due to destruction of emulsion or emulsion and change in product pH when used in water or formulations.

According to another aspect of the present invention, there is provided a cosmetic composition comprising the antimicrobial composition as a preservative component.

The cosmetic composition according to an embodiment of the present invention can be used without limitation in the content range capable of exhibiting the antimicrobial activity of the above-mentioned antimicrobial composition, but can be used in an amount of 0.5 to 5% by weight But it is not limited thereto.

In addition, the cosmetic composition according to one embodiment of the present invention may be prepared in the form of a general emulsified formulation, a solubilized formulation, or the like, in addition to the manufacturing method specifically disclosed in the present invention, using a conventionally known manufacturing method. The cosmetic composition may be appropriately selected according to the purpose. For example, the cosmetic composition may be selected from the group consisting of softening agents, convergent lotion, nutritional lotion, eye cream, nutritional cream, massage cream, cleansing cream, cleansing foam, cleansing water, But the present invention is not limited thereto. The cosmetic composition of the present invention may be formulated into a formulation selected from the group consisting of cosmetics, essences, packs, and the like, and it does not cause creaming phenomenon or flocculation phenomenon due to high- It is not possible to find a phenomenon such as discoloration or swelling caused by the above-mentioned problems.

According to another aspect of the present invention, the present invention provides a natural preservative comprising the antimicrobial composition. That is, the antimicrobial composition according to the present invention can be substituted for a synthetic preservative used in foods, quasi-drugs, etc. in addition to a cosmetic composition.

The natural preservative according to an embodiment of the present invention not only shows antimicrobial spectrum equal to or more than that of conventional parabens, such as a paraben's chemical preservative, but also exhibits a range of use And the limitations of the system are expected to be solved.

In addition, the present invention provides an aqueous dispersion containing the above-mentioned antimicrobial composition. The aqueous dispersion according to an embodiment of the present invention may have a visible light transmittance (Tv) of 90% or more at 400 to 700 nm light ray at a concentration including 10% by weight of the antimicrobial composition based on the total weight of the aqueous dispersion, , Preferably 90 to 99.9%, and more preferably 95 to 99.9%.

Hereinafter, the antimicrobial composition including the natural plant extract according to one example of the present invention will be described in more detail by way of examples. It should be understood, however, that the invention is not limited to the disclosed embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the invention. In addition, the unit of the additives not specifically described in the specification may be% by weight.

(Example 1)

And purified and purified from carbacol and natural materials were purchased and used. Separated and purified from ghazza extract to 90% or more, and the antibacterial composition was prepared according to the composition shown in Table 1 below.

(Example 2)

The pulverized gauze having a dry weight of 100 g was heated and refluxed in an aqueous 70% (v / v) ethanol solution for 12 hours, cooled, and then filtered through a filter paper having a permeation size of 1.2 탆. The filtered extract was concentrated under reduced pressure at 50 DEG C or lower and then completely dried using a vacuum dryer to obtain 50 g of a ghatti extract in solid form (the content of kebulic acid in the Ghadam extract was 18.53%).

Antibacterial compositions were prepared with the compositions shown in the following Table 1, from the Ghassa extract obtained by the above method and the carbaccol isolated and purified from nature.

(Example 3)

The pulverized oregano powder having a dry weight of 100 g was heated and refluxed with a 70% (v / v) aqueous ethanol solution for 12 hours, cooled, and then filtered through a filter paper having a permeation size of 1.2 탆. The filtered extract was concentrated under reduced pressure at 50 DEG C or lower and then completely dried using a vacuum dryer to obtain 9.5 g of a liquid oregano extract (content of carbaglol in oregano extract was 76%).

The oregano extracts obtained by the above method and the kerulonic acids separated and purified from nature were prepared as the composition shown in the following Table 1.

(Example 4)

The ghatti extract obtained by the method of Example 2 and the oregano extract obtained by the method of Example 3 were prepared as shown in Table 1 below.

(Example 5)

100 g of the dry weight of the crushed strawberry greens was heated and refluxed with a 70% (v / v) aqueous ethanol solution for 12 hours, cooled, and then filtered through a filter paper having a size of 1.2 탆 penetration. The filtrate was concentrated under reduced pressure at 50 DEG C or lower, and then completely dried using a vacuum dryer to obtain 12 g of a strawberry herb extract.

An antimicrobial composition was prepared in the same manner except that the antimicrobial composition of Example 1 further contained the strawberry herb extract obtained by the above method. The composition thereof is shown in Table 1 below.

(Example 6)

The pulverized orchids having a dry weight of 100 g were heated and refluxed for 12 hours in an aqueous 70% (v / v) ethanol solution, followed by cooling and filtration with a filter paper having a permeation size of 1.2 mu m. The filtered extract was concentrated under reduced pressure at 50 DEG C or lower and completely dried using a vacuum dryer to obtain 19 g of an orchid extract.

An antimicrobial composition was prepared in the same manner except that the antimicrobial composition of Example 1 further contained the orchid extract obtained by the above method. The composition thereof is shown in Table 1 below.

(Example 7)

The ground green tea having a dry weight of 100 g was heated and refluxed with a 70% (v / v) aqueous ethanol solution for 12 hours, cooled, and then filtered with a filter paper having a size of 1.2 탆 penetration. The filtered extract was concentrated under reduced pressure at 50 DEG C or lower and completely dried using a vacuum dryer to obtain 21 g of green tea extract.

An antimicrobial composition was prepared in the same manner except that the antimicrobial composition of Example 1 further contained the green tea extract obtained by the above method. The composition thereof is shown in Table 1 below.

(Example 8)

The pulverized latex having a dry weight of 100 g was heated and refluxed with an aqueous 70% (v / v) ethanol solution for 12 hours, cooled, and then filtered with a filter paper having a size of 1.2 탆 penetration. The filtered extract was concentrated under reduced pressure at 50 DEG C or lower, and then completely dried using a vacuum dryer to obtain 4 g of anextra extract.

An antimicrobial composition was prepared in the same manner, except that the antimicrobial composition of Example 1 further contained an aftercoat extract obtained by the above method. The composition thereof is shown in Table 1 below.

(Example 9)

The pulverized pulpy having a dry weight of 100 g was heated and refluxed with a 70% (v / v) aqueous ethanol solution for 12 hours, cooled, and then filtered with a filter paper having a size of 1.2 μm. The filtered extract was concentrated under reduced pressure at 50 DEG C or lower, and then completely dried using a vacuum dryer to obtain 10 g of Parsley extract.

An antimicrobial composition was prepared in the same manner except that the antimicrobial composition of Example 1 further contained the persimmon extract obtained by the above method. The composition thereof is shown in Table 1 below.

(Comparative Example 1-2)

Antibacterial compositions were prepared with the compositions of Table 1 below, each containing carbacol or < RTI ID = 0.0 > carblinic < / RTI >

(Comparative Example 3)

An antimicrobial composition was prepared in the same manner as in Example 1, except that the rosemary extract was used instead of the capurinic acid. The composition thereof is shown in Table 1 below.

ingredient Example Comparative Example One 5 6 7 8 9 One 2 3 Carbacol One One One One One One 2 - One Carbulinic acid One One One One One One - 2 - Strawberry herb extract - One - - - - - - - Orchid extract - - One - - - - - - Green tea extract - - - One - - - - - Acacia extract - - - - One - - - One Parsley extract - - - - - One - - - Propanediol To 100

In order to measure the antimicrobial activity of the antimicrobial compositions prepared in the above Examples and Comparative Examples, the minimum inhibitory concentration (MIC) for each microorganism was measured by the following method, and the results are shown in Table 2 below. The antimicrobial activity was measured by the following method and the results are shown in Table 3 below. The cytotoxicity of the antimicrobial composition prepared in Example 1 was evaluated by the following method. The results are shown in Table 4 below.

In addition, the skin irritation test for the antimicrobial composition prepared in the above examples was carried out by the following method, and the results are shown in FIG.

1. Minimum development inhibition concentration ( MIC )

Staphylococcus aureus ATCC 6538P as gram-positive bacteria, Escherichia coli ATCC 8739 as gram-negative bacteria, Pseudomonas aeruginosa as a gram-positive bacterium were used to measure the antibacterial effect of the antimicrobial composition prepared from the above Examples and Comparative Examples. Candida albicans ATCC 10231 and Aspergillus niger ATCC 22343 were purchased from Korea Biotechnology Research Center (KCTC, Korea) as yeast. Nutrient broth solutions were prepared and sterilized at 121 ° C for 15 min. Each of the cultured strains was inoculated into nutrient broth (10 ml) and cultured in a shaking incubator at 24 And cultured for a period of time to prepare a strain for an antibacterial experiment.

In order to measure the minimum inhibitory concentration of the antimicrobial composition inhibiting the growth of the microbial strain, the strain cultured in the sterilized medium was cultured at a concentration of 1 × 10 6 cfu / ml for bacteria (bacteria) and 1 × 10 5 cfu / ml for fungi Were cultured at 35 ° C for 2 days, and fungi were cultured at 25 ° C for 5 days and observed as a control. The antimicrobial composition was added to the medium so as to be 1% by weight, and then sterilized. The bacteria were inoculated at 1 × 10 6 cfu / ml for fungi and 1 × 10 5 cfu / ml for fungi, For 2 days, and fungi were cultured at 25 ° C for 5 days and observed as an experimental group.

To confirm the presence or absence of the growth of each strain in the culture medium of the cultured control and experimental groups, approximately 300 μl of the sample was taken from the aseptic space and dispensed into a sterilized 96-well microplate. Using a spectrophotometer, The minimum inhibitory concentration (MIC) was determined by measuring the minimum concentration at which low proliferation was observed for each strain compared with the control group. The results are shown in the following table 2.

division Minimum development inhibitory concentration (MIC,% v / v) S.aureus E. coli P. aeruginosa C. albicans A. niger Example 1 0.20 0.30 0.40 0.20 0.10 Example 5 0.19 0.25 0.35 0.20 0.08 Example 6 0.15 0.28 0.35 0.20 0.07 Example 7 0.20 0.30 0.35 0.18 0.10 Example 8 0.17 0.25 0.40 0.15 0.12 Example 9 0.15 0.30 0.38 0.20 0.10 Comparative Example 1 0.50 0.60 0.60 0.30 0.20 Comparative Example 2 1.50 2.00 0.50 3.00 2.00 Comparative Example 3 0.40 0.40 0.50 0.30 0.20

As shown in Table 2, the antimicrobial composition according to the present invention exhibited excellent antimicrobial activity at a concentration of 0.3% (v / v) or less with respect to Gram-positive bacteria such as Staphylococcus aureus and Escherichia coli, and 0.4 % (v / v) or less. In particular, it was confirmed that the antibacterial activity against Pseudomonas aeruginosa alone was low when carbacol and carburinic acid were used alone, but the antibacterial activity was remarkably improved when mixed with Ghazana extract. The antimicrobial activity against Candida albicans and Aspergillus niger was observed to be synergistic. In addition, Comparative Example 3, which was prepared by using the extract of Phellinus linteus instead of Kabulic acid, showed no improved effect on the antibacterial effect. That is, the antimicrobial composition according to the present invention may indicate that synergistic antibacterial activity is exhibited by using carbamic acid and carbacol together.

In addition, in the case of an antimicrobial composition substituted with an extract having carbacol as a main indicator substance and an extract having a carbilinic acid as a main indicator substance, in addition to a combination of carbacol and carbulinic acid, synergistic effect on antimicrobial activity Was significantly higher than that of the control group. In the case of Comparative Example 1, which is used alone as carbacol, the solubility in water was so low that it had to be shaken for re-mixing at the time of use.

2. Measurement of antimicrobial activity (preservative activity)

In order to measure the antimicrobial activity of the antimicrobial composition prepared in Example 1, Staphylococcus aureus ATCC 6538P was used as a gram positive bacterium, Escherichia coli ATCC 8739 was used as a gram negative bacterium, Pseudomonas aeruginosa ATCC 9027 Candida albicans ATCC 10231 and Aspergillus niger ATCC 22343 were used as yeast.

Approximately 50 μl of each of the cultivated strains was added to a solidified plate medium, and the mixture was dispensed into a plate culture medium and uniformly smoothed using a spreader. About 40 μl of the antimicrobial composition was absorbed into a paper disk having a diameter of 10 mm, the solvent was dried, the microorganism was closely adhered to the surface of the medium on which the strain had been coated, and cultured in an incubator at 30 ° C. for 48 hours or more. And the size of the clear zone formed in the test piece was measured to confirm the antibacterial effect. Since microorganisms can not grow near the paper disk, the larger the average diameter value, the better the antimicrobial activity. The results are shown in Table 3, and the results are shown in Table 3. The results are shown in Table 3. The results are shown in Table 3 below.

division S.aureus E. coli P. aeruginosa C. albicans A. niger Average diameter (mm) Example 1 28.50 26.15 10.80 31.00 41.70 27.63 DMSO 6.45 6.45 6.45 6.45 6.45 6.45 Propanediol (PD) 6.45 6.45 8.20 6.45 6.45 6.80 Methyl paraben (MP) 12.40 23.40 16.40 23.60 38.55 22.87

As shown in Table 3, it was found that the antimicrobial composition according to the present invention exhibits excellent antibacterial activity in various combinations including carbacol and couulic acid. In particular, it was confirmed that the antifungal activity of Staphylococcus aureus and Candida albicans was excellent.

3. Assessment of cytotoxicity

The cell toxicity in HaCaT cells and HDF-N cells was measured using Example 1, which is the basis of the antimicrobial composition according to the present invention. HaCaT cells were seeded at a density of 5 × 10 4 cells per well in a 96-well plate and at a density of 6 × 10 3 cells per well in a 96-well plate for HDF-N cells. The medium was removed and washed with DPBS. After 12 hours of starvation, the test solution and fresh medium (except media supplement) were added and cultured for 24 hours. After washing with PBS, the WST-1 reaction solution was diluted 1/10 in the supplemented medium and treated with 100 μl of each solution. , And the absorbance was measured at 450 nm. As a result, survival rates of HaCaT cells and HDF-N cells were 93.1% and 98.3%, respectively, at the maximum concentration of 5%. Accordingly, the antimicrobial composition according to the present invention is expected to be able to exhibit antimicrobial activity effectively by substituting a chemical preservative for cosmetic composition, food, and pharmaceutical formulation (see Table 4).

sample Control group Experimental group [cytotoxicity against HaCaT cells] density 0 0.01 0.1 0.5 1.0 2.0 5.0 Control (%) 100.0 100.5 99.4 98.3 98.2 97.4 93.1 sample Control group Experimental group [cytotoxicity against HDF-N cells] density 0 0.01 0.1 0.5 1.0 2.0 5.0 Control (%) 100.0 100.2 99.4 99.2 98.8 98.1 97.3

4. Skin irritation test of antimicrobial composition

Using Example 1, which is the basis of the antimicrobial composition according to the present invention, the antimicrobial composition was diluted to 5 wt% and 10 wt% in purified water to conduct a skin irritation test. Toxicologically, 11 healthy adults were conducted according to the CTFA guidelines (The Cosmetic, Toxic and Fragrance Association, Inc. Washington, DC, 20036, 1991). After dropping 20 μl of the sample and solution into a finn chamber, the sample and the solution were placed on the arm of the human body to be tested and fixed with a tape. After 24 hours, 48 hours, and 72 hours of application, the patches were removed, and after 2 hours, the skin response of the test site was evaluated according to the following criteria (no 0-stimulation; 0.5- 1 - mild irritation such as weak erythema, 2 - pronounced erythema accompanied by papule and edema, and 3: severe irritation such as severe erythema accompanied by blisters). At this time, purified water was used as a control group of the skin irritation experiment.

As a result, all of the antimicrobial compositions according to the present invention were found to have a stimulation index of 0 due to no stimulation (see Fig. 1).

(Examples 10-12)

A cosmetic composition (serum) was prepared with the composition shown in Table 5 below. The raw materials 1 to 4 were added to purified water, stirred at room temperature (23 ° C) for 10 minutes, and the raw material 5 was added to prepare a transparent serum preparation.

(Comparative Example 4)

A transparent serum preparation was prepared in the same manner as in Example 10, except that methylparaben was used in the same amount instead of the antimicrobial composition prepared in Example 1.

Raw material name Example Comparative Example 10 11 12 4 One GLYCERINE 7.5 2 DISODIUM EDTA 0.02 3 HYDROXYETHYL CELLULOSE 0.5 4 PEG-60 HYDROGENATED CASTOR OIL 0.4 5 Example 1 1.0 - - - Example 5 - 1.0 - - Example 6 - - 1.0 - METHYL PARABEN - - - 1.0 6 WATER To 100

(Examples 13-15)

A cosmetic composition (cream-in-water type formulation) was prepared with the composition shown in Table 6 below. The raw materials 1 to 3 were heated and adjusted to 70 ° C to prepare a water component (phase A), and the raw materials 4 to 12 were heated and adjusted to 70 ° C to prepare a milk ingredient (phase B). The oil component was added to the water component, homogenized for 3 minutes with a homomixer (3000 rpm), defoamed, filtered and cooled to prepare an underwater type cream formulation.

(Comparative Example 5)

An underwater type cream formulation was prepared in the same manner as in Example 13, except that the same amount of methylparaben was used instead of the antimicrobial composition prepared in Example 1.

Raw material name Example Comparative Example 13 14 15 5 PHASAYA One WATER To 100 2 GLYCERINE 5.0 3 DISODIUM EDTA 0.02 PHASE B 4 HYDROGENATED DICTION 7.0 5 PENTAERYTHRITYL TETRAETHYLHEXANOATE 2.0 6 CETEARYL ALCOHOL 1.5 7 GLYCERYL STEARATE (and) PEG-100 STEARATE 1.5 8 SORBITAN SESQUIOLEATE 1.0 9 GLYCERYL STEARATE 0.7 10 DIMETHICONE 1.0 11 SODIUM POLYACRYLATE (and) C18-C21 ALKANE (and) TRIDECETH-6 0.3 12
Example 1 3.0 - - - Example 5 - 3.0 - - Example 6 - - 3.0 - METHYL PARABEN - - - 3.0

 The antibacterial effect of the cosmetic composition containing the antimicrobial composition prepared in the above Examples was measured by the following method, and the results are shown in Table 7 below.

5. Confirmation of antibacterial effect of cosmetic composition

To confirm the antimicrobial effect of the cosmetic composition containing the antimicrobial composition, the test method of the microbiology guidelines of the Cosmetic, Toility and Fragrance Association, Inc. (CTFA) was used. Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, and Candida albicans were used as gram-positive bacteria, Staphylococcus aureus ATCC 6538P, Gram-negative bacteria, (Candida albicans ATCC 10231), and Aspergillus niger ATCC 22343 were used.

A suspension in which three kinds of bacteria (E. coli, P. aeruginosa, S. aureus) and two kinds of fungi (C. albicans, A. niger) were mixed was injected into a vial containing 10 g of each cosmetic composition, / g and 1 × 10 5 cfu / g, and then 1 g of the sample was taken under sterile conditions at 4 hours, 1 day, 2 days, 3 days, and 7 days after storage at 25 ° C. and appropriately diluted with a diluent Bacteria were cultured on Nutrient agar (Difco) and fungi were cultured on potato dextrose agar (Difoc) medium at 35 ℃ and 25 ℃ for 24 ~ 72 hours, respectively. Respectively.

ingredient Fungi D-value (hr) Example 10 Germ 5.2 Fungus 5.1 Example 11 Germ 5.3 Fungus 5.8 Example 12 Germ 5.1 Fungus 5.2 Example 13 Germ 7.4 Fungus 9.3 Example 14 Germ 7.1 Fungus 9.2 Example 15 Germ 7.4 Fungus 9.2 Comparative Example 4 Germ 5.2 Fungus 5.2 Comparative Example 5 Germ 7.5 Fungus 9.3

* D-value: the time required for 90% microbial killing

As shown in Table 7, it was confirmed that the antiseptic effect of the cosmetic composition containing the antimicrobial composition according to the present invention is equivalent to or better than the comparative example using the conventional chemical preservative (methylparaben). That is, when the antimicrobial composition according to the present invention is used in a cosmetic composition, it is possible to realize an excellent antibacterial effect.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, Those skilled in the art will recognize that many modifications and variations are possible in light of the above teachings.

Accordingly, the spirit of the present invention should not be construed as being limited to the embodiments described, and all of the equivalents or equivalents of the claims, as well as the following claims, belong to the scope of the present invention .

Claims (13)

An antibacterial composition comprising carbacol and cerulonic acid as active ingredients. The method according to claim 1,
An antibacterial composition comprising an oregano extract containing carbacol and a carburonic acid as an active ingredient. The method according to claim 1,
An antimicrobial composition comprising an oregano extract containing carbacol and a potato extract containing the above carbomeric acid as an active ingredient. The method according to claim 1,
Wherein the antimicrobial composition further comprises one or more extracts selected from the group consisting of Lilium extract, green tea extract, orchid extract, strawberry herb extract, and Parsley extract. The method according to claim 1,
Wherein the antimicrobial composition further comprises at least one selected from glycerin, propanediol, butanediol, hexanediol, octanediol, and methylpropanediol. The method according to claim 1,
Wherein the antimicrobial composition has an antimicrobial activity against a microorganism selected from bacteria and fungi. 6. The method of claim 5,
Wherein the microorganism is at least one selected from the group consisting of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, Aspergillus niger, Bacillus megaterium, and Exopiala monileri. The method according to claim 6,
Wherein the antimicrobial composition has a minimum development inhibitory concentration (MIC) of 0.1 to 100 占 퐇 / ml for the microorganism. 9. A cosmetic composition comprising an antimicrobial composition according to any one of claims 1 to 8 as a preservative. 10. The method of claim 9,
Wherein the cosmetic composition comprises 0.1 to 10% by weight of the antimicrobial composition. 10. The method of claim 9,
Wherein the cosmetic composition is formulated with a softening agent, a convergent lotion, a nutritional lotion, an eye cream, a nutritional cream, a massage cream, a cleansing cream, a cleansing foam, a cleansing water, a powder, an essence or a pack. A natural preservative comprising an antimicrobial composition according to any one of claims 1 to 8. 8. An aqueous dispersion according to any one of claims 1 to 8, wherein the aqueous dispersion has a visible light transmittance of 90% or more at a wavelength of 400 to 700 nm, wherein the aqueous dispersion contains 5.0% by weight of the antimicrobial composition.

Images