KR101427462B1 - Composite Natural Antiseptics From Forsythia suspensa Vahl, Leonurus sibiricus L., Crataegi fructus and Akebia quinata Decaisne, Cosmetic Compositions Containing Them - Google Patents

Abstract

The present invention relates to a complex natural preservative consisting of an alginate, an alginate, a sorghum extract and a barberry extract, and a cosmetic composition comprising the same as an active ingredient, and more particularly to a natural preservative comprising an almond extract, The antimicrobial activity and the antibacterial effect are excellent without addition of preservative, and thus the antimicrobial activity exhibits an excellent effect as a preservative. At the same time, the antimicrobial activity is exhibited and safety is confirmed in the human body. Can be useful in.

Description

   (Composite Natural Antiseptics From Forsythia suspensa Vahl, Leonurus sibiricus L., Crataegi fructus and Akebia quinata Decaisne, Cosmetic Compositions Containing Them), which is an active ingredient,

The present invention relates to a composite natural preservative comprising a combination of an alginate, an alginate, an acid anhydride and a gum extract and a cosmetic composition comprising the same as an active ingredient. More particularly, the present invention relates to a composite natural preservative comprising an alginate, It is an invention that can be usefully used in various fields such as preservative, cosmetics, household goods and the like because it is excellent in antibacterial and repellency without addition of preservative.

Many products such as foods, medicines, and cosmetics, which are widely used in daily life, contain essential preservatives in order to prevent changes in internal properties and to preserve them for a certain period of time. It is also common to add chemical preservatives to prevent contamination during storage as well as storage and contamination during use.

Some of the materials used as the preservatives are derived from natural sources, but most of them are chemically synthesized artificial materials. Preservatives that are safely used as cosmetics and general-purpose medicines are limited because of their use as skin allergies and potential for environmental hormones. Therefore, they are limited in their content and use formulations . As one example, most cosmetics contain purified water, so there is a high likelihood of contamination of the bacteria and the product is likely to become rotten due to chemical changes. To prevent this, the artificial preservatives parabens, imidazolidinyl urea, methylchloroisothiazolyl P-toluenesulfonic acid, p-toluenesulfonic acid, p-toluenesulfonic acid, phenoxyethanol, and the like.

As mentioned above, the risk of such artificial preservatives is rising, and many related companies feel the necessity of development of natural preservatives, but actually they are not used due to the insufficient price and antibacterial ability. In the case of natural active substances, there is a problem that a large amount thereof is required to be used for reasons of color, stability and antibacterial activity in a narrow region. Therefore, the natural active substances are partially commercialized, and hinokitiol, Extracts such as magnolol and grapefruit extract are only partially available.

 As a prior art relating to the present invention, Patent No. 10-825450 discloses a cosmetic composition for improving skin wrinkles containing a tangle extract, Patent Document 10-2011-121365 discloses a natural surfactant composition containing an extract of chrysanthemum extract, And 10-816264 disclose cosmetic compositions for relieving skin irritation which contain an extract of Ganoderma lucidum as an active ingredient. These cosmetic compositions are each a cosmetic composition containing an alum, The composition of the present invention is significantly different from the composite natural preservative of the present invention and the cosmetic composition has insufficient antimicrobial activity to a satisfactory degree. Thus, the composition disclosed in Japanese Patent Application Laid-Open No. 10-2011-65151 includes a composition This study was conducted to investigate the effects of the scopolamine on the learning ability and spatial perception ability Exhibits an excellent effect of improving the other at all is given to the compound a natural preservative in the present invention but is disclosed to be useful for improving forgetfulness and degenerative brain diseases.

Accordingly, a composite natural preservative having sufficient preserving power without any adverse effects on the skin and a cosmetic composition containing the same have been developed.

The inventors of the present invention conducted studies on a complex natural preservative having a wide range of antimicrobial active agent-like properties while reducing the above-mentioned problems, and using the plant extracts having reported antimicrobial activity as an antimicrobial activity, As well as a wide range of human-safe natural herbicides, and applies them to cosmetic compositions containing them.

The present invention provides a complex natural preservative characterized by comprising a combination of an alginate, a glutinous herb, a sorghum, and a root extract.

In addition, the present invention provides a complex natural preservative characterized in that the combination of alfalfa, sorghum, sorghum, and garlic extract is contained at a ratio of 1: 1: 1: 5.

In addition, the present invention provides a complex natural preservative characterized in that the combination of alfalfa, sorghum, sorghum, and sorghum is contained in a ratio of 1: 2: 2: 3.

Also, the present invention provides a cosmetic composition, wherein the complex natural preservative contains 0.05 to 10.0% by weight of the active ingredient based on the total weight of the composition.

Finally, the present invention relates to a cosmetic composition, wherein the cosmetic composition is selected from the group consisting of a cosmetic lotion, a gel, a water-soluble liquid, a milk lotion, a nutritional cream, a massage, a cream, an essence, an ointment, a cleansing foam, a cleansing water, a shampoo, a rinse, A makeup base, a makeup base, a foundation, a skin cover, a makeup product, a hair pack, and a hair essence.

The complex natural preservative of the present invention exhibits a broad and excellent antibacterial activity against various microorganisms, especially bacteria, fungi and yeast, and the cosmetic composition containing the same has excellent antibacterial, repellency and preservative ability, There is an advantage of not showing acute oral toxicity.

Hereinafter, the present invention will be described in detail.

As a raw material for the natural preservative of the present invention, Yeon-gyo (翹 翹) is a medicinal herb of Oriental herbaceous perennials, and has a characteristic odor. It is also used to lower fever and decline heart fever in the early stage of fever, to use for fever and mental complaints, and to use for boils, rashes, appendicitis, lungs, lymphadenitis and sore throat. Antimicrobial activity, anti-inflammatory effect, hypotensive effect, hemostatic effect, liver treatment effect, fever, pain, diuretic action were reported by pharmacological action.

In addition, 夏草 grass is a biennial plant belonging to the family Lamiaceae, called 母子草. Good for blood circulation, amenorrhea, menstrual pain, postpartum uterine contraction deficiency bleeding, abdominal pain when there is a blood ((瘀血) and helps the contraction of the uterus. It has a light diuretic effect, so it is used when the amount of urine is low and it does not come out well, when the body is swollen, and it is used for eczema, itching boil. The washed-up stamen has physiological control function and visual acuity enhancement function.

In addition, it is a plant of hawthorn which softens rough skin and removes acne and freckles, and the triterpene saponin ingredient helps to lower cholesterol. It is known to be good for arterial sclerosis and hypertension.

Finally, the tree is called Akebia quinata Decaisne ) or other stem plants, which are made by cutting off the skin and cutting it horizontally. This medicine is not poisonous, but it tastes good and its quality is cold. It is used for the pain of swelling in the mouth due to dehydration of the bladder due to pyelonephritis, cystitis, urethritis, deep rash in the mouth, heartburn, red urine, paralyzed pain. Pharmacological action has been reported diuretic, fungicide action, and promote postpartum lactation, lactation is good.

The artichoke, cherry, sorghum and thistle extract according to the present invention can be prepared as follows.

Firstly, after cleaning the alum, alum, sorghum, and sachet each with water, dry it in the shade, and dry alum, alum, sorghum, and sachet are crushed. Each of these pulverized products is put into an extraction container, and then water, alcohol, or a mixture of these is put in a solvent, and the mixture is then deposited and extracted at a suitable temperature for a certain period of time. The extract thus obtained is filtered and concentrated. In the case of extracting alfalfa, sorghum, sorghum, and garlic using organic solvents, alcohols such as methanol, ethanol, isopropanol and butanol, alcohols such as ethylene, acetone, ether, hexane, chloroform, ethyl acetate, N, (DMF), dimethylsulfoxide (DMSO), and mixtures thereof, at room temperature or with warming, using an organic solvent. As the extraction solvent, it is preferable to use water, alcohol or a mixed solvent thereof. In the above extraction, 10 to 50 parts by weight, preferably 10 to 30 parts by weight, of the crosslinking agent, the herbicide, the herbicide and the herder are respectively applied to the solvent. When the amount is less than 10 parts by weight, excess solvent is used There is difficulty in economical efficiency and concentration, and when it exceeds 50 parts by weight, it is difficult to be sufficiently impregnated into the solvent, so that the extraction is not easy.

   The extraction method may be an extraction method such as a heat extraction method, a reflux cooling extraction method, an ultrasonic extraction method and the like. Preferably, the method can be extracted once or twice by a heat extraction method. It becomes meaningless as an efficacious ingredient.

   The above-mentioned immersion temperature is 30 to 50 ° C, more preferably 45 ° C, and it is most effective in preventing discoloration and deterioration in this temperature range. When the temperature is less than 30 ° C, the extraction period becomes long, There is a problem of. The immersion time is suitably 3 days to 7 days, preferably 5 days. When the drying time is less than 3 days, the extraction of the desired component is insufficient. When the drying time exceeds 7 days, the extraction component is not changed and the extract is discolored. Leachate temperature is 80 ~ 90 ℃ and leaching time is 5 ~ 6 hours.

After obtaining the above-mentioned ligatures, extracts, sorghum extracts and root extracts, the solids are removed by using a filter paper or the like, and the filtrate is filtered with a 0.2 to 0.5 탆 filter, and the supernatant is filtered under reduced pressure. The above-obtained soft palate, dried persimmon, sorghum extract, and sorghum extract can each be provided as an extract having excellent antibacterial activity and having activity as a preservative.

   Preferably, the natural preservative of the present invention is contained in a ratio of 1: 1: 1: 5 or 1: 2: 2: 3. In addition, the present invention provides a cosmetic composition for natural preservatives, which comprises 0.05 to 10.0% by weight, based on the total weight of the composition, as an active ingredient. If the content of the extract is less than 0.05% by weight based on the total weight of the composition, it is difficult to exert the expected preservative effect. If the content exceeds 10.0% by weight, further increase in preservative efficacy is not expected.

   Finally, the natural preservative cosmetic composition of the present invention, which comprises the active ingredient of the extract of the present invention, the extract, the sorghum, the sorghum and the thymus, can be contained in various products such as cosmetics, medicines, foods, textiles, household goods, Use is preferred. More specifically, the present invention relates to a composition for use in skin, which is selected from the group consisting of convergent lotion, cream, nutritional essence, foundation, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, Lt; / RTI > The formulations of cosmetics may also be presented in the form of, for example, solutions, gels, solid or emulsions, suspensions, microemulsions, creams, skins, lotions, powders, ointments obtained by dispersing the oil phase in water. The cosmetic composition may contain various conventional carriers and additives which are suitable for each of the above-mentioned formulations and are well known in the art.

Hereinafter, the structure and function of the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples, but includes modifications of equivalent technical ideas.

Manufacture of chrysanthemum, chrysanthemum, chrysanthemum, chrysanthemum extract

The herbs, chrysanthemums, chrysanthemums, and corn syrup were collected in Korea. Wash the collected herbals thoroughly, remove the foreign substances, dry, crush them, and filter them in 10mesh. After 100 g of each dried, chopped, crushed, ice-cold and acidified milk were obtained, they were immersed in 1000 ml of a solvent (ethanol: purified water = 75: 25) and leached in a 45 ° C thermostat for 5 days. The resulting extract was cooled to room temperature, filtered through a sieve, and filtered. The filtrate was concentrated by using a rotary vacuum concentrator to obtain 200 g of each of the extracts of chrysanthemum, shaman, ice, and corn oil.

 Manufacture of allium, thrush, tree and extract

The same procedure as in Example 1 was followed except that the alum, chaff and neck were replaced with lees for 5 days instead of leaching for 5 days in a 45 ° C thermostat 200 g each of alum, alum, neck, and extract were obtained.

<Experimental Example 1> Experiment 1

The disk diffusion method according to the Kirby-Bauer method was used to measure the buoyancy using the extracts obtained in Examples 1 and 2, respectively. This method is a general antimicrobial measurement method in which a plurality of discs are immersed in a solution of an antimicrobial substance at a predetermined concentration and then cultured on a solid agar medium coated with the test strain, and then the size of the transparent area formed around the disc is measured. Escherichia coli ATCC 25922, Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 6538 and Candida albicans ATCC 10231 were used as the strains.

                           Antimicrobial spectrum (Unit: mm) E. coli B. subtilis S. aureus P. aeruginosa C. albicans Abalone 9.5 10 11.5 10 12.0 And seconds 12.5 11.3 9.0 8 9 Sansa 10.5 14 15 6.0 5 Thong 10 8 10 11 14 Ice 8.0 10.0 4.3 7.2 6.3 Corn oil 10 11 7.8 7.5 5 Neck and neck 5.5 7.3 6.2 3.5 3.4

* Size of transparent bar: 3mm or less: no effect,

  The size of the transparent bar 4 ~ 10mm:

  Size of transparent bar 11mm or more: Excellent effect

As shown in Table 1, the extract of Yeast extract showed excellent antimicrobial activity against Bacillus subtilis and Staphylococcus aureus, and showed excellent antimicrobial activity against P. aeruginosa and Candida yeast. In the case of the herbal extract, it showed excellent antimicrobial activity against Bacillus subtilis and Escherichia coli, but the antimicrobial activity against P. aeruginosa was weak.

In the case of Sansa extract, it showed excellent antimicrobial activity only against Bacillus subtilis and Staphylococcus aureus. The extracts of Bacillus thuringiensis showed excellent antimicrobial activity against all bacteria such as Escherichia coli, Pseudomonas aeruginosa, Candida yeast.

&Lt; Experimental Example 2 >

Each of the test bacteria previously cultured in the prepared medium was inoculated into each Petri dish at a final concentration of about 5 × 10 ⁶ CFU, and in the case of yeast, the bacteria were cultured at a concentration of 5 × 10 ⁵ CFU In a Petri dish. Bacteria are cultured at 37 ° C for 48 hours in each Petri dish. Yeasts are cultured at 25 ° C for 3 days and then observed for colony visually. The minimum inhibitory concentration (MIC) is defined as the minimum antimicrobial concentration of the plate in which the growth of the microorganism is inhibited.

                           Antimicrobial spectrum (Unit:%) E. coli B. subtilis S. aureus P. aeruginosa C. albicans Abalone 1.3 1.2 0.5 0.8 0.7 And seconds 0.6 0.4 1.3 1.0 2.0 Sansa 1.0 0.5 0.4 0.7 1.0 Thong 0.6 1.0 0.8 0.7 0.5

* Judgment standard: 0.6% or less - Excellent

As shown in Table 2, in the case of Yeast Extract, the effect of Bacillus subtilis and E. coli was small, but it was very good for S. aureus and yeast. , And the extracts showed relatively strong antimicrobial activity against Bacillus subtilis and E. coli. The extracts of Glycyrrhizae japonica were relatively effective against Gram - positive bacteria, Staphylococcus aureus and Staphylococcus aureus.

 Manufacture of complex natural preservatives

Among the natural extracts selected from the antibacterial efficacy tests of the various extracts prepared in Examples 1 and 2, Yeast extract, which has excellent antimicrobial activity, And mixed various natural preservatives as shown in Table 3 below. In the comparative example, methylparaben and phenoxyethanol, which are chemical substances, were mixed with purified water to prepare a chemical preservative.

     Mixture ratio of preservative by extract and chemical (unit: gr) Conduct No. Abalone And seconds Sansa Thong Methyl paraben Phenoxyethanol Purified water One 0.5 0.5 0.5 0.5 - - 98.0 2 0.25 0.25 0.75 0.75 - - 98.0 3 1.25 0.25 0.25 0.25 - - 98.0 4 0.25 0.25 1.25 0.25 - - 98.0 5 0.25 1.25 0.25 0.25 - - 98.0 6 0.25 0.25 0.25 1.25 - - 98.0 7 0.25 0.5 0.75 0.5 - - 98.0 8 0.5 0.25 0.5 0.75 - - 98.0 9 0.25 0.5 0.5 0.75 - - 98.0 Comparative Example - - - - 0.15 0.2  99.65

<Experimental Example 3> Buoyancy test in the formulation

In order to examine the repellency of the natural preservative containing the extracts, a preservative activity confirmation test was carried out for Examples 1 to 9 and Comparative Examples. It is very important to make a stable product by testing the effectiveness of preservatives.

Escherichia coli ATCC 9637, Pseudomonas aeruginosa ATCC 9027, and Candida yeast were used as gram-positive bacteria, such as Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538, (Candida albicans ATCC 10231) was used.

In the pre-culture, the bacterium was inoculated to a nutrient agar and the yeast was inoculated to a YM agar. Bacteria were cultured at 26 to 37 ° C for 24 hours and yeast were cultivated for 48 hours at 25 ° C in a culture tank. The bacteria were suspended in 0.8% physiological saline to obtain bacteria at a concentration of about 1 × 10 ^{6 to 7,} CFU / ⁵ CFU / g.

100 g of each of the essence base samples of Examples 1 to 9 and Comparative Example was prepared, and 1 ml of the mixed suspension of the above four bacteria and one yeast was inoculated thereto. For each sample inoculated after this, 1 g is taken and diluted in 9 ml of PBS solution to make a dilution series by 10 times. 100 μl of each dilution is injected into the agar medium and spread by a sterilizing rod. The medium is then inverted and the bacteria are counted after incubation at 37 ° C for 24 hours and the yeast at 25 ° C for 3 days.

Due to buoyancy effectiveness, bacteria should be reduced by 99.9% or more within 7 days of inoculation, and there should be no proliferation during the test period. Yeast and fungi should be inoculated with 1 × 10 ⁵ (cells / ㎖) of fungus, and should be reduced by at least 90% within 7 days and should not be proliferated during the test period. The results are shown in Tables 4 and 5.

Number of bacteria (cells / ml) 3 days 1 week 2 weeks 3 weeks 4 weeks Embodiment No. 1 5 x 10 ³ 1 x 10 ³ 1 x 10 ² <100 0 Embodiment No. 2 6 × 10 ⁵ 2 x 10 ³ 1 x 10 ⁴ 2 x 10 ² <100 Embodiment No. 3 6 × 10 ⁵ 1 × 10 ⁵ 8 × 10 ² <100 <100 Embodiment No. 4 3 x 10 ³ 1 x 10 ² <100 <100 0 Embodiment No. 5 4 × 10 ⁴ 2 x 10 ³ 1 x 10 ² 3 × 10 ² <100 Embodiment No. 6 2 x 10 ⁴ 1 x 10 ² <100 0 0 Embodiment No. 7 2 x 10 ² 1 x 10 ² <100 0 0 Embodiment No. 8 3 x 10 ³ 3 × 10 ² <100 <100 0 Embodiment No. 9 1 x 10 ² <100 0 0 0 Comparative Example 2 x 10 ² <100 0 0 0

As shown in Table 4, Examples 1 to 5 showed weak preservative activity in the death of bacterium, but in Examples 6 to 9, all of them died after 7 days, It was concluded that the antioxidant activity of Yeast extract, Acanthopanax senticosus extract and Acantha sp.

Fungi (cells / ml) 3 days 1 week 2 weeks 3 weeks 4 weeks Embodiment No. 1 1 × 10 ⁵ 4 × 10 ⁴ 9 × 10 ² <100 0 Embodiment No. 2 1 × 10 ⁵ 2 x 10 ⁴ 1 x 10 ⁴ 8 × 10 ² <100 Embodiment No. 3 5 × 10 ⁴ 2 x 10 ³ 2 x 10 <100 0 Embodiment No. 4 8 × 10 ³ 3 x 10 ³ 2 x 10 ² <100 0 Embodiment No. 5 7 x 10 ⁴ 4 × 10 ⁴ 2 x 10 ² <100 0 Embodiment No. 6 7 x 10 ⁴ 3 × 10 ² <100 <100 0 Embodiment No. 7 4 x 10 ³ 1 x 10 ³ 3 × 10 ² 0 0 Embodiment No. 8 1 x 10 ⁴ 2 x 10 ³ 8 × 10 ² <100 0 Embodiment No. 9 3 x 10 ³ 1 x 10 ² <100 0 0 Comparative Example 2 x 10 ³ 2 x 10 ² <100 0 0

As shown in Table 5, the effects of Examples 1, 2, 3, 4, 5 and 7 were insignificant and Examples 6, 8 and 9 were similar to those of Comparative Example of chemical preservative.

&Lt; Preparation Example > Effect of cosmetics containing a complex natural preservative

In order to measure the preservative effect of the cosmetic composition containing the complex natural preservative prepared in Example 3, various formulations containing a complex natural preservative at an appropriate concentration were prepared as follows.

<Manufacturing Example 1> Convergent lotion

As shown in Table 6 below, a convergent lotion containing a natural preservative was prepared according to a conventional method.

ingredient content(%) function  1,3-butylene glycol 5.0  Moisturizer  Polyoxyethylene (60) Hardened castor oil 0.5  Surfactants  glycerin 2.0  Moisturizer  Spices 0.1  air freshener  ethanol 4.0  Barrier strengthening agent  Sodium hyaruronate 0.2  Skin protection  EDTA-2Na 0.02  Skin conditioner  Allantoin 0.1  Skin protection  Purified water Remainder  Skin conditioner  Natural preservative 1.5  Preservative

&Lt; Preparation Example 2 >

The softening water containing the natural preservative was prepared according to a conventional method as shown in Table 7 below.

ingredient content(%) function  1,3-butylene glycol 7.0  Moisturizer  Cetostearyl alcohol 0.1  Emollients  Polyoxyethylene lauryl ether 0.5  Surfactants  Monostearyl polyoxyethylene sorbitan 1.5  glycerin 5.0  Moisturizer  Glyceryl monostearate 1.5  Emollients  Sunflower seed oil 8.0  Emollients  Spices 0.1  air freshener  Natural preservative 2.0  Preservative  cholesterol 0.5  Skin protection  Ceramide 0.2  Stearic acid 0.8  Skin conditioner  KOH 0.05  corrector  Carbomer 0.1  Incrementer  Purified water Remainder  Skin conditioner

<Manufacturing Example 3> Essence

Essences containing natural preservatives were prepared according to the conventional method as shown in Table 8 below.

ingredient content(%) function  1,3-butylene glycol 7.0  Moisturizer  Polyoxyethylene lauryl ether 0.5  Surfactants  Monostearyl polyoxyethylene sorbitan 1.5  glycerin 15.0  Moisturizer  Squalane 3.0  Emollients  Spices 0.1  air freshener  Natural preservative 1.5  Preservative  Ceramide 0.2  Skin protection  Beta Glucan 1.0  Skin protection  KOH 0.2  corrector  Carbomer 0.2  Incrementer Hyaluronic acid (1.0%) 5.0  Skin protection  Purified water Remainder  Skin conditioner

&Lt; Preparation Example 4 >

Creams containing natural preservatives were prepared according to the conventional method as shown in Table 9 below.

ingredient content(%) function  Squalane 5.0  Emollients  vaseline 2.0  Emollients  Self emulsifying glycerin monostearate 2.5  Ancillary surfactant  glycerin 8.0  Moisturizer  Spices Suitable amount  air freshener  1,3-butylene glycol 10.0  Liquid paraffin 3.0  Emollients  beeswax 1.0  Microstellin wax 1.0  Skin protection  Cetearyl alcohol 2.0  Emollients  Glyceryl stearate 2.0  Natural preservative 2.0  Preservative  Cetearyl glucoside 2.0  Surfactants  Ceramide 0.3  Skin protection  Stearic acid 1.0  Skin conditioner  KOH 0.3  corrector  Magnesium aluminum silicate 0.4  Incrementer

Claims (5)

delete Wherein the extract is extracted at a temperature of 45 占 폚 for 5 days, and the extracts of Lycopersicon esculentum and Lycoris spp. Are separately contained at a ratio of 1: 1: 1: 5 at 5 ° C for 5 days.
Wherein the extract is leached for 5 days at a temperature of 45 ° C, and the extracts of Lycopersicon esculentum, Lycopersicon esculentum and Lycopersicon esculentum extracted at 85 ° C for 5 days are contained in a ratio of 2: 2: 1: 3.
The method according to claim 2 or 3,
Wherein the composite natural preservative is present in an amount of 0.05 to 10.0% by weight based on the total weight of the composition.
5. The method of claim 4,
The cosmetic composition may be in the form of a cosmetic composition such as a cosmetic lotion, a gel, a water-soluble liquid, a milk lotion, a nutritional cream, a massage, a cream, an essence, an ointment, a cleansing foam, a cleansing water, a shampoo, a rinse, a pack or a body oil, , A skin cover, a makeup product, a hair pack, and a hair essence.