KR20160105722A

KR20160105722A - Cosmetic composition with effect of proliferation of lactic acid bacteria and anti-bacterial activity and manufacturing method thereof

Info

Publication number: KR20160105722A
Application number: KR1020160035509A
Authority: KR
Inventors: 박미영
Original assignee: 박미영
Priority date: 2016-03-24
Filing date: 2016-03-24
Publication date: 2016-09-07
Also published as: KR101674512B1

Abstract

The present invention relates to a cosmetic composition comprising an antibacterial aqueous solution containing a non-mineral natural mineral component and a composite inorganic salt extracted from a plant and having excellent antibacterial activity and a wide range of antimicrobial spectrum, It has excellent antimicrobial effect and is extracted from natural materials. It is not only safe for human body because it has no cytotoxicity and skin irritation, but it also proliferates not only beneficial bacteria such as lactic acid bacterium, but also protects skin from atmospheric drying, ultraviolet rays and various pollutants Not only can help maintain health, but also has anti-aging efficacy to prevent skin aging such as wrinkle improvement or prevention.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition having excellent antibacterial activity and proliferation ability for lactic acid bacteria,

The present invention relates to an antibacterial and antifungal agent which is not only cytotoxic but also has excellent antimicrobial and antifungal functions by including an antibacterial aqueous solution extracted from a plant as an active ingredient and has antibacterial activity which can contribute to the proliferation and protection of skin lactic acid bacteria, To a cosmetic composition having excellent ability to proliferate lactic acid bacteria and a method for producing cosmetics using the same.

Many products such as foods, medicines, and cosmetics widely used in daily life are used to prevent changes in internal properties and to prevent the organic matter from decaying due to the action of microorganisms, Preservatives are added to prevent proliferation.

In particular, in the case of cosmetics, contamination by microorganisms occurring during the manufacturing process, contamination by bacteria on the surface due to skin contact of fingers during use, or contamination by other substances such as water introduced from the outside during use can not be avoided Therefore, cosmetic compositions generally contain preservatives for the purpose of inhibiting the growth of microorganisms and preventing changes in the physical properties of the composition during use and preventing contamination. When preservatives are not formulated, A complicated means is required, such as requiring aseptic manufacturing processes, limiting the amount of content or duration of use, or making containers specially, which tends to lack economical versatility.

Most of commonly used preservatives are chemically synthesized chemical components. Among them, preservatives that are most stable among them and which are generally used for cosmetics and pharmaceuticals include, for example, paraoxybenzoic esters , Imidazolidinyl urea, phenoxyethanol, methylchloroisothiazolinone, methylisothiazolinone, chlorophenesin and the like. However, even the preservatives of these parabens are excellent in efficacy and stability as preservative measures, Skin irritation, irritation, skin allergies, potential as an environmental hormone, and inducing resistant bacteria.

Therefore, the trend is to use 1,2-hexanediol (1,2 hexane-diol) or xylitol, which is a non-paraffinic preservative. Even if cosmetics contain very small amounts of preservatives, The use of antiseptics is being curtailed due to the tendency to prohibit excessive use of antimicrobial agents and cognition to distinguish them from products.

In addition, frequent use of antiseptics or antibiotics that inhibit the killing or proliferation of bacteria results in the development of resistant strains that survive or are resistant to mutation, resulting in more resistant strains and more powerful antibiotics Which eventually leads to bacteria that can resist any powerful antibiotic, called superbacteria.

Super bacteria is a bacteria that can resist the frequent use of antibiotics and is resistant to strong antibiotics. In 1961, MRSA ( Methicillin- Resistant Staphylococcus Aureus ) was used in the UK and VRSA ( Vancomycin -Resistant Staphylococcus Aureus , vancomycin-resistant Staphylococcus aureus) was first reported.

MRSA [ methicillin resistant staphylococcus aureus ] is called methicillin resistant Staphylococcus aureus. It is known to be found in a large general hospital that uses antibiotics as a major cause of hospital infection. It is said to have a strong survival ability and fertility such as a body part of the air, doctors and nurses, a scalpel, a hospital blanket, have.

However, in the case of the non-paraffinic preservative, the effect of the preservative is deteriorated because the non-paraffinic preservative is rather proliferated in the case of super bacteria such as MRSA.

Therefore, many people feel the necessity of natural preservatives, and studies on natural preservatives which are excellent in safety and economy continue, but in reality, they are expensive, have a weak antimicrobial effect, , There is a problem in that the use of a high dose causes deterioration in safety due to discoloration, dehairing, and skin irritation, which is a limitation in application to actual products.

In the registered patent No. 1393008 (registered on Apr. 13, 2014), a quinoid compound, which is a spectral extract-derived compound obtained by supercritical extraction of the outpost of a crowd, as an active ingredient, Compositions are presented.

In the registered patent No. 1427462 (Registered on Aug. 31, 2014), a combined natural preservative exhibiting excellent antimicrobial activity against bacteria, fungi and yeast by including dried persimmon, extracts obtained by leaching chrysanthemum, A cosmetic composition that exhibits excellent antibacterial, repellency, and preservative properties without addition of a chemical preservative and is free from cytotoxicity is produced by producing a composite natural preservative.

However, this prior art differs from the present invention in that it has an antibacterial function against MRSA which is a superbacteria, and a lactic acid bacterial growth effect, which is a beneficial bacteria.

Registration No. 1393008 (Registered on March 13, 2014) Registration No. 1427462 (Registration Notice)

The present invention relates to an antimicrobial composition containing an antibacterial aqueous solution extracted from a plant as an active ingredient and having excellent antimicrobial effect and natural antimicrobial effect without cytotoxicity, And to provide a cosmetic preparation method using the same.

In order to achieve the above object, the present invention provides a cosmetic composition having excellent antibacterial activity and proliferating ability of lactic acid bacteria, and is preferably a cosmetic composition containing an antibacterial aqueous solution, a moisturizer, a conditioning agent, a surfactant, a thickener, a pH adjusting agent, At least one cosmetic ingredient from the group consisting of and purified water or water.

The antibacterial aqueous solution may contain 0.001 to 10 wt% based on the total weight of the cosmetic composition, and the cosmetic composition may further include a chemical preservative.

Wherein the antibacterial aqueous solution contains at least one mineral selected from the group consisting of calcium (Ca), potassium (K), magnesium (Mg), sodium (Na), silicon (Si), aluminum (Al), iron (Fe) and P (phosphorus); And chloride ion (Cl ^-), bromide ion (Br ^-) and one or more of the sulfide ions (S ^2-), nitrate ion (NO ₃ ^-), sulfate ion (SO ₄ ^{- -),} carbonate ions (CO ₃₎ It is more preferable that the minerals in the antibacterial aqueous solution contain 5 to 35: 0.1 to 20: 2 to 20: 1 to 25 by weight of calcium: potassium: magnesium: sodium.

The composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, oil, soap, cleansing, shampoo, rinse, oil, powder foundation, emulsion foundation, wax foundation, .

Another embodiment of the present invention is a method for producing an antibacterial aqueous solution, comprising the steps of: extracting a plant to prepare an antibacterial aqueous solution; Preparing a mixture by adding at least one cosmetic ingredient in purified water or water to at least one cosmetic ingredient selected from the group consisting of a moisturizer, a conditioning agent, a surfactant, a pH adjuster, an antioxidant and a fragrance; Mixing the mixture with the antibacterial aqueous solution; And an antimicrobial solution is mixed with purified water or a water and / or a thickening agent to control the viscosity. The present invention also provides a method for producing a cosmetic composition having excellent antibacterial activity and ability to proliferate a lactic acid bacterium.

The step of preparing the mixture is preferably uniformly mixed at a temperature of 50 to 100 ° C at 2000 to 3000 rpm for 10 to 20 minutes.

The mixing may be carried out by mixing the mixture at a temperature of 50 to 100 ° C. for 10 to 100 minutes so that the antibacterial aqueous solution is 0.001 to 10 wt% based on the total weight of the cosmetic composition, or the mixture is cooled to a temperature of 20 to 49 ° C. , The antibacterial aqueous solution may be added to the mixture for 10 to 100 minutes such that the antibacterial aqueous solution is 0.001 to 10 wt% based on the total weight of the cosmetic composition.

The present invention relates to a cosmetic composition comprising an antibacterial aqueous solution containing a non-mineral natural mineral component and a composite inorganic salt extracted from a plant and having excellent antibacterial activity and a wide range of antimicrobial spectrum, It also shows excellent antibacterial effect.

In addition, it is not only cytotoxic and skin irritation, it is not only safe for the human body but also helps to proliferate not only the lactic acid bacteria which are beneficial bacteria of the human body, but also protect the skin health from the atmospheric drying, ultraviolet rays and various pollutants And it has an anti-aging effect that prevents skin aging such as wrinkle improvement or prevention.

1 to 8 are test results of a human repeat insult patch test (HRIPT) of a dermatological test of an antibacterial aqueous solution prepared according to an embodiment of the present invention.
9 and 10 are anti-fungal test results of an antibacterial aqueous solution prepared according to an embodiment of the present invention.
11 to 19 are antibacterial test results of an antibacterial aqueous solution prepared according to an embodiment of the present invention.
FIG. 20 is a test report on the experiment of multiplying the antibacterial aqueous solution, the cosmetic composition containing 1% of the antibacterial aqueous solution and the cosmetic composition containing 1% of the non-paraffinic chemical preservative according to another embodiment of the present invention.
21 and 22 are anti-fungal test reports of a cosmetic composition containing 1% of an antibacterial aqueous solution prepared according to another embodiment of the present invention.
23 to 25 are antibacterial test results of MRSA of a cosmetic composition containing 1% of an antibacterial aqueous solution prepared according to another embodiment of the present invention.
26 to 28 are antibacterial test results of MRSA in a cosmetic composition containing 1% of a non-paraffinic chemical preservative prepared according to the comparative example of the present invention.
29 is an antibacterial test report of a cosmetic composition containing 1% of an antibacterial aqueous solution prepared according to another embodiment of the present invention.
30 is a cytotoxicity test report of a cosmetic composition containing 1% of an antibacterial aqueous solution prepared according to another embodiment of the present invention and a cosmetic composition containing 1% of a non-paraffinic chemical preservative.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings. Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. As well as the fact that

Throughout this specification, when an element is referred to as "including" an element, it is understood that it may include other elements as well, without departing from the other elements unless specifically stated otherwise.

In each step, the identification code is used for convenience of explanation, and the identification code does not describe the order of the steps, and each step may be performed differently from the stated order unless clearly specified in the context. have. That is, each of the steps may be performed in the same order as described, or may be performed substantially concurrently or in the reverse order.

The term "extract" in this specification is meant to encompass all of the natural materials obtained by extracting components from plants, regardless of the extraction method, extraction solvent, extracted components or extract form, and the minerals constitute the human body Refers to all elements other than oxygen, carbon, hydrogen, and nitrogen, which are the main constituents of organic matter among the elements required for physiological activities such as growth and maintenance.

First, the cosmetic composition having excellent antibacterial activity and lactic acid bacterial growth ability of the present invention is prepared by mixing at least one cosmetic component and purified water from the group consisting of an antibacterial aqueous solution, a moisturizer, a conditioning agent, a surfactant, a thickener, a pH adjusting agent, Water.

The antibacterial aqueous solution is a colorless and odorless aqueous solution containing a non-mineral natural mineral component and a complex inorganic salt. The extract extracted from a plant can be used as it is without reprocessing. Preferably, the antibacterial aqueous solution is separated and purified by using an ultrafiltration membrane having a constant molecular weight cut- They can be separated by various chromatographies (made for separation according to size, charge, hydrophobicity or affinity), fermented in a natural state or using various microorganisms, various purification methods, and reworked through active fractions.

The antibacterial aqueous solution may contain at least one mineral selected from the group consisting of calcium (Ca), potassium (K), magnesium (Mg), sodium (Na), silicon (Si), aluminum (Al), iron (Fe) chloride ion (Cl ^-), bromide ion (Br ^-), nitrate ion (NO ₃ ^-), sulfate ion (SO ₄ ^-), carbonate ions (CO ₃ ^-) and sulfide ions (S ₂ ^-) at least one anion of And may be mixed with an inorganic salt or an aqueous solution containing the minerals and the anion.

Preferably, the mineral in the antibacterial aqueous solution may contain calcium: potassium: magnesium: sodium in a weight ratio of 5 to 35: 0.1 to 20: 2 to 20: 1 to 25.

More preferably, the amount of the solute contained in 1 L of the above-mentioned antibacterial aqueous solution is 20 to 90 mg, and the main components are 2,560 to 8,900 g / l of calcium (Ca) and 55 to 4,636 g / l of potassium (K) , Magnesium (Mg) of 1,107 to 5,483 占 퐂 / l, sodium (Na) of 334 to 4,868 占 퐂 / l and carbonic acid ion (CO ₃ ^- ) of 1,760 to 6,900 占 퐂 / l.

The above-mentioned antibacterial aqueous solution is an extract derived from natural plants and is a colorless and odorless non-mineral natural mineral component. When such an antibacterial aqueous solution is contained in a cosmetic composition, it can inhibit the growth of microorganisms and prevent the change of the physical properties of the composition during the use period and prevent contamination, and can also contribute to the proliferation and protection of skin lactic acid bacteria, which are beneficial bacteria in the body. And may be included in an amount of 0.001 to 10 wt% based on the total weight of the composition.

The cosmetic composition of the present invention may further contain a chemical preservative generally used in the cosmetics or foods for human body in order to prevent decay by bacteria and yeast bacteria so as to have an excellent antimicrobial activity and a broader range in antimicrobial spectrum .

The above-mentioned chemical preservative is not particularly limited as long as it is a chemical preservative used in cosmetic composition, and it is possible to use the cosmetic composition according to the mode of use, method of use, and form of the cosmetic composition of the present invention, .

On the other hand, when the cosmetic composition containing the antibacterial aqueous solution, cosmetic ingredient and water is prepared without the chemical preservative, the antibacterial aqueous solution may be used in an amount of preferably 0.3 to 1 wt% based on the total weight of the cosmetic composition If the content of the antibacterial aqueous solution is less than 0.3 wt%, antimicrobial effect is not expected to be expected. If the content is more than 1 wt%, it is difficult to expect an increase in antibacterial effect.

However, when a chemical preservative is contained in the cosmetic composition or a cosmetic ingredient contains a component that can irritate the skin, the content of the antibacterial aqueous solution is included in the range of 0.001 to 10 wt%, thereby widening the antimicrobial spectrum and enhancing the antimicrobial activity Skin irritation and cytotoxicity can be reduced and eliminated.

The cosmetic composition contained in the cosmetic composition is not particularly limited as long as it is a component included in a commonly used cosmetic composition and can be used in all cases. Preferably, the cosmetic composition includes a moisturizer, a conditioning agent, a surfactant, a thickener (thickening agent or viscosity adjusting agent) At least one or more ingredients selected from the group consisting of an antioxidant, a controlling agent, an antioxidant and a fragrance may be used. The addition and content of the components may be adjusted depending on the formulation and function of the cosmetic composition of the present invention, And may be included in appropriate adjustment.

Examples of the moisturizing agent include dipropylene glycol (DPG), glycerine, allantoin, jojoba oil as a conditioning agent, stearic acid, cetyl alcohol cetanol, aracel-165, aracel-83 and carbopol 941 as the thickening agent.

For example, the cosmetic composition having excellent antibacterial activity of the present invention may contain 3-5 wt% of dipropylene glycol (DPG), 3-5 wt% of glycerine, arginine 0.01 to 0.2 wt% of allantoin, 0.1 to 1 wt% of soybean extract, 0.1 to 3 wt% of stearic acid, 1 to 3 wt% of cetanol, 0.1 to 3 wt% of Cell-165, 0.1 to 3 wt% of Twin-60, 0.1 to 1 wt% of Aracel-83, 1 to 5 wt% of jojoba oil, 1 to 3 wt% of silicone (Silicon 1202), 1 to 3 wt% of mineral oil, 1 to 5 wt% of arginine oil, 0.01 to 0.1 wt% of carbopol 941, 0.01 to 0.2 wt of vitamin E %, Flavor 0.1 to 0.3 wt%, and antibacterial aqueous solution 0.3 to 1 wt%.

The cosmetic composition having excellent antibacterial activity and proliferative power of lactic acid bacteria of the present invention may be prepared in any form conventionally produced in the art, and examples thereof include solutions, suspensions, emulsions, pastes, gels, creams, lotions, But are not limited to, oils, soaps, cleansing, shampoos, rinses, oils, powder foundations, emulsion foundations, wax foundations and sprays.

When the formulation of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used in the cosmetic composition. In particular, in the case of a spray, a mixture of chlorofluorohydrocarbons , Propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a dissolving agent or an emulsifying agent is used in the cosmetic composition, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like.

When the formulation of the present invention is a soap, a cleansing, a shampoo, a rinse, especially a cleansing agent containing an interface-active agent, the cosmetic composition may contain an aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative , Methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester.

The cosmetic composition of the present invention can also be applied to everyday household goods such as mask packs, fibers, wet tissues and the like.

In another embodiment of the present invention, there is provided a method for producing an antimicrobial aqueous solution, comprising the steps of: extracting plants to prepare an antibacterial aqueous solution; adding water to at least one cosmetic component selected from the group consisting of a moisturizer, a conditioning agent, a surfactant, a pH adjusting agent, A step of mixing the antibacterial solution with the mixture, and a step of adjusting the viscosity of the mixed solution containing the antibacterial solution to prepare a cosmetic composition having excellent antibacterial activity.

The step of preparing the antibacterial aqueous solution may comprise preparing an antibacterial aqueous solution by mixing the plant extracts extracted from the respective plants at a certain ratio or preparing an antibacterial aqueous solution by mixing the plants at a predetermined ratio and extracting them at once .

Preferably, in order to obtain an antibacterial aqueous solution from a plant, an extract is obtained from plants through various extraction methods such as solvent extraction, reflux cooling extraction, ultrasound extraction, purification extraction, active fraction extraction or fermentation extraction, and the mixture is subjected to filtration, Spray drying, sterilization, fermentation, and the like can be further added.

The step of preparing the mixture includes preparing at least one cosmetic component from the group consisting of a moisturizer, a conditioning agent, a surfactant, a pH adjuster, an antioxidant, and a perfume in purified water or water, Is not particularly limited as long as it is a component included in a cosmetic composition used as a cosmetic composition, and can be used in the form of a moisturizer, a conditioning agent, a surfactant, a thickener (thickening agent or viscosity adjusting agent), a pH adjusting agent, May be used. The addition amount and the content of each component may be suitably adjusted in a pharmaceutically effective amount or a suitable amount depending on the formulations and functions of the cosmetic composition having excellent antibacterial activity of the present invention.

Preferably, the step of preparing the mixture comprises adding Dipropylene Glycol (DPG), glycerine, arginine, allantoin and soybean extract to purified water or water at a temperature of 50 to 100 ° C (2000 to 3000 rpm), and then stearic acid, cetanol, aracel-165, Twin-60, aracel-83, Jojoba oil, silicone (Silicon 1202), mineral oil and arginine oil were added and mixed uniformly for 10 to 20 minutes to prepare a mixture.

The specific composition to be contained in the cosmetic composition having excellent antibacterial activity has been mentioned above, and thus will not be described here.

Preferably, the mixing is performed so that the antibacterial aqueous solution is contained in the mixture at a temperature of 50 to 100 DEG C for 10 to 100 minutes so that the antibacterial aqueous solution is 0.001 to 10 wt% based on the total weight of the cosmetic composition, , The antibacterial aqueous solution may be mixed in an amount of 0.001 to 10 wt% based on the total weight of the cosmetic composition.

In order to adjust the viscosity according to the manner and method of using the mixture containing the antibacterial aqueous solution, the viscosity is controlled by mixing water and a thickener such as carbopol 941 (carbopol 941), and then vitamin E or fragrance The cosmetic composition of the present invention having excellent antibacterial activity and proliferating ability of lactic acid bacteria can be prepared.

Hereinafter, the present invention will be described in more detail with reference to examples of the present invention. However, the scope of the present invention is not limited to the following preferred embodiments. Those skilled in the art can implement various modified embodiments of the present invention within the scope of the present invention.

[Production Example 1]

Preparation of antibacterial aqueous solution

The plants collected from nature were dried, immersed in a solvent, heated at a temperature of 100 ° C or less for a certain period of time, and filtered to obtain an antibacterial aqueous solution.

1 to 8 show results of evaluation of skin irritation and skin irritation by the method of HRIPT (Human Repeat Insult Patch Test). A total of 53 persons were subjected to the antibacterial aqueous solution and the antibacterial aqueous solution was impregnated The patches were attached for 48 hours at first, then attached to the skin for 3 times a week for 24 hours, and the skin was stimulated for a total of 3 weeks. As a result, it was confirmed that the prepared antibacterial aqueous solution had no irritation and no sensitivity.

[Experimental Example 1]

Antimicrobial Antifungal And antibacterial experiments

1. Antifungal test

10 ml of the antibacterial solution prepared in Preparation Example 1 was inoculated into 90 ml of the test strain mixed in a test environment of 29.0 ± 0.2 ° C at a concentration of 99.0 ± 1.0% RH, allowed to stand for 24 hours, and cultured for 5 days. Respectively.

At this time, the test strains were black mold Aspergillus niger (ATCC 9642), Penicillium pinophilum ATCC 11797), Chaetomium globosum ATCC 6205, Gliocladium < RTI ID = 0.0 > virens ATCC 9645), Aureobasidium pullulans ATCC 15233) were mixed and used.

2. Antibacterial test

10 ml of the antibacterial aqueous solution prepared in the above Preparation Example was placed in an Erlenmeyer flask and sterilized at 121 ° C for 15 minutes using a sterilizer. Then, 90 ml of physiological saline (NaCl 8.5%) and 1 ml of the cultured test strain were added. (The test strain was used in a Nutrient broth culture.) After shaking culture at 37.0 ± 0.1 ° C in a 31.7 ± 0.2% RH environment, a certain amount of the strain was collected and plated on an agar medium, and the number of bacteria was measured .

At this time, as a test strain, antibiotic resistant bacteria MRSA ( Staphylococcus aureus subsp . aureus ATCC 33591), Candida aldicans ATCC 10231), Pseudomonas aeruginosa ATCC 15442, Salmonella typhimurium IFO 14193, Escherichia coli cili ATCC 25922), Staphylococcus aureus ATCC 6538, and Klebsiella pneumoniae ATCC 4352 were used.

As a control, 100 ml of physiological saline was used to culture the test strain by the same test method as described above, and the number of bacteria was measured.

Test Items Test result Antifungal test Mold growth Initial concentration
(CFU / ml) After 24 hours concentration (CFU / ml) Bacterial reduction rate
(%) Antibacterial test MRSA Control group 1.2 x 10 ⁴ 4.1 x 10 ⁴ - Group with addition of antibacterial aqueous solution 1.2 x 10 ⁴ <10 99.9 Candida Control group 1.4 x 10 ⁴ 3.7 × 10 ⁴ - Group with addition of antibacterial aqueous solution 1.4 x 10 ⁴ <10 99.9 P. aeruginosa Control group 1.7 x 10 ⁴ 7.0 x 10 ⁴ - Group with addition of antibacterial aqueous solution 1.7 x 10 ⁴ <10 99.9 Salmonella
Control group 1.6 x 10 ⁴ 6.2 × 10 ⁴ - Group with addition of antibacterial aqueous solution 1.6 x 10 ⁴ <10 99.9 Escherichia coli Control group 1.4 x 10 ⁵ 2.4 × 10 ⁶ - Group with addition of antibacterial aqueous solution 1.4 x 10 ⁵ &Lt; 1.0 x 10 ³ 99.9 Staphylococcus Control group 2.4 × 10 ⁵ 3.1 × 10 ⁶ - Group with addition of antibacterial aqueous solution 2.4 × 10 ⁵ &Lt; 1.0 x 10 ³ 99.9 Pneumococcus Control group 1.2 × 10 ⁵ 2.3 × 10 ⁶ - Group with addition of antibacterial aqueous solution 1.2 × 10 ⁵ &Lt; 1.0 x 10 ³ 99.9

9 and 10, it was confirmed that the antimicrobial aqueous solution prepared in Preparation Example 1 of the present invention showed that the mold was undeveloped after 24 hours in the antifungal test, and the results of FIGS. 11 to 19 In the antimicrobial test, the number of microorganisms increased in the control group after 24 hours from the inoculation, whereas in the sample containing the antibacterial solution of the present invention, Candida, Pseudomonas, Salmonella, Escherichia coli, Staphylococcus, It was confirmed that all the bacteria tested were reduced by 99.9%.

[Production Example 2]

Cosmetics Preparation of composition

Dipropylene Glycol (DPG), glycerine, arginine, allantoin and soybean extract were dissolved by heating at 60 ° C in water at the same ratio as in Table 2, And then mixed with stearic acid, cetanol, aracel-165, Twin-60, aracel-83, jojoba oil, silicone Silicon 1202), mineral oil and arginine oil were added thereto, and the mixture was homogeneously mixed for 10 minutes and cooled to 40 ° C. Thereafter, the antimicrobial aqueous solution prepared in Preparation Example 1 or a non-paraffinic chemical preservative as a preservative was added and uniformly mixed. Then, a predetermined viscosity was adjusted by adding carbopol 941 (viscosity increasing agent) and water. Then, the mixture was cooled to room temperature, and vitamin E and fragrance were added thereto, followed by mixing with an agi mixer and defoaming to prepare Comparative Examples 1 to 2 and Examples 1 to 4 as cosmetic compositions.

Comparative Example 1 Example 1 Example 2 Example 3 Example 4 Comparative Example 2 Purified water 73.62 73.72 73.92 74.12 74.42 74.42 Dipropylene glycol
(DPG) 4.00 4.00 4.00 4.00 4.00 4.00 glycerin 4.00 4.00 4.00 4.00 4.00 4.00 Arginine 0.30 0.30 0.30 0.30 0.30 0.30 Allantoin 0.10 0.10 0.10 0.10 0.10 0.10 Soybean extract 0.50 0.50 0.50 0.50 0.50 0.50 Stearic acid 1.00 1.00 1.00 1.00 1.00 1.00 Cetyl alcohol 1.80 1.80 1.80 1.80 1.80 1.80 ARASEL-165 0.80 0.80 0.80 0.80 0.80 0.80 Twin-60 1.20 1.20 1.20 1.20 1.20 1.20 Aracel-83 0.50 0.50 0.50 0.50 0.50 0.50 Jojoba oil 3.00 3.00 3.00 3.00 3.00 3.00 silicon 2.00 2.00 2.00 2.00 2.00 2.00 Mineral oil 2.00 2.00 2.00 2.00 2.00 2.00 Arginine
oil 3.00 3.00 3.00 3.00 3.00 3.00 Carapril 941 0.08 0.08 0.08 0.08 0.08 0.08 Vitamin E 0.10 0.10 0.10 0.10 0.10 0.10 Spices 0.20 0.20 0.20 0.20 0.20 0.20 Antibacterial solution 0.20 0.30 0.50 0.70 1.00 - Non-paraffin system
Chemical preservative - - - - - 1.00 Sum 100 100 100 100 100 100

^{(Unit: kg)}

[Experimental Example 2]

Depending on the content of the antibacterial aqueous solution Cosmetics Antimicrobial test of composition

10 g of the cosmetic composition prepared in Preparation Example 2 was placed in an Erlenmeyer flask and sterilized at 121 ° C for 15 minutes using a sterilizer. Then, 90 ml of physiological saline (NaCl 8.5%) and 1 ml of the cultured test strain were added. (The test strain was used in a Nutrient broth culture.) After shaking culture at 37.0 ± 0.1 ° C in a 31.7 ± 0.2% RH environment, a certain amount of the strain was collected and plated on an agar medium, and the number of bacteria was measured .

The antimicrobial effect was evaluated as the antimicrobial effect when the number of bacteria remaining in the medium decreased to 99.9% or less after 24 hours compared to the initial number of bacteria injected.

Comparative Example 1 Example 1 Example 2 Example 3 Example 4 Comparative Example 2 Initial input
Number of bacteria 1.3 × 10 ⁶ After 24 hours
Number of bacteria 1.5 x 10 ³ 1,250 320 250 0 210

When the antibacterial aqueous solution was contained in an amount of more than 0.3 wt% in the cosmetic composition, the antibacterial activity was 99.9%. In Example 4 containing 1 wt% The antibacterial aqueous solution of the present invention alone contains 0.3 to 1 wt% of the antibacterial aqueous solution in the cosmetic composition when it is used alone. , Respectively.

However, when mixed with a chemical preservative in a cosmetic composition or mixed with a toxic raw material, the content of the antibacterial aqueous solution may be 0.001 to 10 wt%.

[Experimental Example 3]

Cosmetics Cytotoxicity test of composition

To investigate the effect of the cosmetic composition of Example 4 and Comparative Example 2 on RAW 264.7 cell proliferation, cell viability was measured by MTT reduction assay. RAW 264.7 cells (1.0 × 10 4 cells / ml), and the cells were cultured in a 5% CO2 incubator at 37 ° C. for 24 hours. After diluting the cells of Example 4 and Comparative Example 2 to 10% Each cell line was treated with 1%, 3%, 5%, and 10% of the final concentration in the medium, treated with 1% DMSO in the control, and cultured for 48 hours. After adding 10 μL of MTT (5 mg / mL) solution dissolved in PBS buffer, the formazan formation was confirmed by reacting for 30 minutes. After removing the medium completely, 100 μL of DMSO was added and the absorbance at 540 nm Were measured. Relative cell viability was determined when the survival rate of the control cells cultured without the treatment of Example 4 and Comparative Example 2 was taken as 100%.

cell
Survival rate
(%) density One % 3% 5% 10% Example 4 76% 70% 55% 30% Comparative Example 2 65% 55% 40% 0 %

The results of Table 4 and FIG. 30 show that the cosmetic composition containing the antibacterial aqueous solution of the present invention is less cytotoxic than the cosmetic composition using the non-paraffinic preservative.

[Experimental Example 4]

Cosmetics Antibacterial experiment of composition

1. Antifungal Experiment

10 g of the cosmetic composition of Example 4 was inoculated into 90 ml of the test strain mixture in a test environment of 29.0 ± 0.2 ° C at 99.0 ± 1.0% RH, allowed to stand for 24 hours, and cultured for 5 days to determine the growth of the fungus .

2. Antibacterial experiment

10 g of the cosmetic composition of Example 4 and Comparative Example 2 was placed in an Erlenmeyer flask and sterilized at 121 DEG C for 15 minutes using a sterilizer. Then, 90 ml of physiological saline (NaCl 8.5%) and 1 ml Respectively. (The test strain was used in a Nutrient broth culture.) After shaking culture at 37.0 ± 0.1 ° C in a 31.7 ± 0.2% RH environment, a certain amount of the strain was collected and plated on an agar medium, and the number of bacteria was measured .

At this time, as a test strain, antibiotic resistant bacteria MRSA ( Staphylococcus aureus subsp . aureus ATCC 33591), Candida aldicans ATCC 10231 ), Staphylococcus aureus ATCC 6538, Klebsiella pneumoniae ATCC 4352) was used.

Test Items Test result Remarks Antifungal test Mold growth - Initial concentration
(CFU / ml) After 24 hours concentration (CFU / ml) Bacterial reduction rate
(%) Antibacterial test MRSA Control group 1.1 x 10 ⁴ 4.3 × 10 ⁴ - Initial concentration
prepare
391% proliferation Example 4 1.1 x 10 ⁴ <10 99.9 - Comparative Example 2 1.1 x 10 ⁴ 4.1 x 10 ⁴ - Initial concentration
prepare
373% proliferation Staphylococcus Example 4 1.3 × 10 ⁶ 0 99.9 - Comparative Example 2 2.1 x 10 ² 99.9 - Candida Example 4 1.1 × 10 ⁶ <10 99.9 - Comparative Example 2 <10 99.9 -

As shown in Table 5 and FIGS. 21 and 22, it was confirmed that the mold was not grown in the antifungal test.

In the antibacterial test, the results of Table 5 and FIGS. 23 to 28 show that the antibacterial activity of Candida and Staphylococcus was better than that of Comparative Example 2 containing non-paraffinic preservative in Example 4 containing an antibacterial aqueous solution.

In the experiment using MRSA bacteria of the super bacteria, the results of Table 5 and FIGS. 26 to 28 of Comparative Example 2 were rather proliferated in FIGS. 23 to 28, but in Example 4, the concentration of bacteria was less than 10 CFU / ml after 24 hours And it was confirmed that it almost died. In the test evaluation shown in Fig. 29, which is another experiment of Example 4, the bacterial concentration was <10 CFU / ml after 24 hours at an initial concentration of 1.3 x 104 CFU / ml, and it was confirmed that the bacterial reduction rate was 99.9% there was.

[Experimental Example 5]

Lactic acid bacteria growth experiment

Lactic acid bacteria ( Leuconostoc mesenterides . ) Was cultured in a liquid medium, and the cultured lactic acid bacteria were diluted to give an initial bacterial concentration of 1.5 x 10 5 CFU / mL. Then, 0.1 ml of the bacterial solution was added to 10 ml of Example 4 and Comparative Example, After 24 hours of incubation, the number of lactic acid bacteria was measured and the survival rate of lactic acid bacteria was checked.

However, when the cultured lactic acid bacteria were diluted, the test was performed by neutralizing with D / E Neutralizing Broth (DIFCO). When the lactic acid bacteria were proliferated in the medium, the number of bacteria on the medium was multiplied by the dilution factor, In the case where the lactic acid bacteria did not proliferate, it was multiplied by the dilution factor <10 (less than 10).

Initial concentration
(CFU / ml) After 24 hours concentration (CFU / ml) Bacterial survival rate
(%) Example 4 1.0 × 10 ⁵ 2.5 x 10 ⁵ 250 Comparative Example 2 12 0.00008

As shown in Table 6 and FIG. 20, in Example 4 of the present invention, lactic acid bacteria, which are beneficial bacteria of the human body, were found to proliferate after 24 hours.

Thus, the results of Experimental Examples 1 to 5 above show that the antibacterial aqueous solution of the present invention is a colorless and odorless non-mineral natural minerals component extracted from plants, and has excellent antimicrobial and antifungal functions without cytotoxicity when used in a cosmetic composition In addition, it can contribute to the proliferation and protection of skin lactic acid bacteria, which are beneficial bacteria in the human body.

Claims

An antibacterial aqueous solution extracted from plants;
At least one cosmetic ingredient selected from the group consisting of a moisturizing agent, a conditioning agent, a surfactant, a thickener, a pH adjusting agent, an antioxidant and a perfume; And
Purified water, or water.

The method according to claim 1,
Wherein the antibacterial aqueous solution is contained in an amount of 0.001 to 10 wt% with respect to the total weight of the cosmetic composition, wherein the antimicrobial aqueous solution is excellent in antibacterial activity and ability to proliferate lactic acid bacteria.

3. The method of claim 2,
Wherein the cosmetic composition further comprises a chemical preservative, wherein the cosmetic composition is excellent in antibacterial activity and ability to proliferate lactic acid bacteria.

The method according to claim 1,
The above-
At least one of minerals of calcium (Ca), potassium (K), magnesium (Mg), sodium (Na), silicon (Si), aluminum (Al), iron (Fe) and phosphorus (P); And
Chloride ion (Cl ^-), bromide ion (Br ^-), nitrate ion (NO ₃ ^-), sulfate ion (SO ₄ ^-), carbonate ions (CO ₃ ^-) and sulfide ions (S ^2-) one or more anions of ; Wherein the cosmetic composition has excellent antibacterial activity and proliferation ability of lactic acid bacteria.

5. The method of claim 4,
Wherein the mineral in the antibacterial aqueous solution comprises calcium: potassium: magnesium: sodium in an amount of 5 to 35: 0.1 to 20: 2 to 20: 1 to 25 by weight, and has excellent antimicrobial activity and ability to proliferate lactic acid bacteria.

6. The composition according to any one of claims 1 to 5, wherein the composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, oil, soap, cleansing, shampoo, rinse, oil, powder foundation, A liquid foundation, a wax foundation, and a spray, wherein the cosmetic composition has excellent antibacterial activity.

Extracting plants to produce an antibacterial aqueous solution;
Preparing a mixture by adding at least one cosmetic ingredient in purified water or water to at least one cosmetic ingredient selected from the group consisting of a moisturizer, a conditioning agent, a surfactant, a pH adjuster, an antioxidant and a fragrance;
Mixing the mixture with the antibacterial aqueous solution; And
A method for producing a cosmetic composition having excellent antibacterial activity and ability to proliferate a lactic acid bacterium, comprising the steps of: mixing a mixed solution containing an antibacterial aqueous solution with purified water or water and / or a thickening agent to control viscosity.

8. The method of claim 7,
The step of preparing the mixture comprises:
Wherein the cosmetic composition is uniformly mixed at a temperature of 50 to 100 占 폚 at 2000 to 3000 rpm for 10 to 20 minutes.

8. The method of claim 7,
Wherein the mixing comprises:
Wherein the antibacterial solution is mixed with the antibacterial aqueous solution at a temperature of 50 to 100 占 폚 for 10 to 100 minutes so that the antibacterial aqueous solution becomes 0.001 to 10 wt% based on the total weight of the cosmetic composition.

8. The method of claim 7,
Wherein the mixing comprises:
Wherein the mixture is cooled to a temperature of 20 to 49 캜 and then the antibacterial solution is mixed with the cosmetic composition in an amount of 0.001 to 10% by weight based on the total weight of the cosmetic composition for 10 to 100 minutes. &Lt; / RTI >