KR20090015374A - Antibacterial mineral water composition and method of producing the same - Google Patents

Abstract

An antibacterial mineral water composition, and a method for preparing the composition are provided to obtain the mineral water composition used for sterilizing or washing the fresh food, the food material, etc. An antibacterial mineral water composition comprises Mg, Ca and K as a mineral component. Preferably the antibacterial mineral water composition comprises deep sea water as an active ingredient, and the ratio of Mg : Ca : K is 3 : 0.1-3 : 0.1-3 by weight. Preferably the antibacterial mineral water composition has a hardness of 80-3,000.

Description

Antimicrobial mineral water composition and method of producing the same

The present invention relates to an antimicrobial mineral water composition and a method for manufacturing the same, and more particularly, antibacterial minerals useful as preparation water or raw material for the production of products requiring antibacterial functions such as water for sterilization and washing, such as fresh foods and food materials, food and beverage, cosmetics, pharmaceuticals, etc. It relates to an aqueous composition and a method for producing the same.

Recently, the consumption of fresh foods such as vegetables and fruits is rapidly increasing due to the change in dietary patterns, but fresh foods are difficult to sterilize under various conditions including heating and sterilization. In addition, foodstuffs handled in the food industry are always at risk of deterioration and deterioration, which may contaminate food poisoning bacteria during food processing and production. For this reason, the KFDA has enacted legislation to allow ozone water, hypochlorous acid, and chlorine dioxide production equipment to be used in group meal sites such as schools for sterilization and washing of fresh foods such as vegetables and fruits. However, ozone water, hypochlorous acid water, chlorine dioxide water production apparatus, etc. are expensive to install and use chemicals, and thus have disadvantages that are not environmentally desirable.

Accordingly, it is an object of the present invention to provide an antimicrobial mineral water composition for sterilization and washing treatment of fresh food, food ingredients and the like and a method for producing the same.

Another object of the present invention is to provide an antimicrobial mineral water composition which is environmentally, economically and hygienically preferable in antimicrobial activity and microbial growth inhibition, and a method for producing the same.

In order to achieve the above object, the present invention provides an antimicrobial mineral water composition containing Mg, Ca and K as a mineral component. Here, the antimicrobial mineral water composition includes deep sea water as an active ingredient, and preferably contains minerals such that the weight ratio of Mg: Ca: K is in the range of 3: 0.1-3: 0.1-3. In addition, the content of sodium ions contained in the mineral water composition is 0.05 to 1.5 times (weight ratio) of the magnesium ion content, the hardness of the mineral water composition is preferably 80 to 3000. The present invention also provides a method for preparing a water supply system comprising: preparing, from a marine deep water raw water, concentrated water in which the ionic components of the raw water are concentrated, and permeate having reduced ionic components from the raw water; And calcium salt, sodium salt, sulfate salt, potassium salt salt and magnesium salt are separated from the concentrated water, and the sodium chloride component is added by adding one or more of the obtained calcium salt salt, potassium salt salt and magnesium salt to the permeate. It provides a method for producing an antimicrobial mineral water composition comprising the step of reducing, and increasing the content of other mineral components.

Hereinafter, the present invention will be described in more detail.

The antimicrobial mineral water composition according to the present invention contains Mg, Ca and K as mineral components, and preferably includes desalted deep sea water as an active ingredient. Generally, deep sea water means seawater located in the deep sea of more than 200m deep where sunlight does not reach, and the deep-water council of the Japan Fisheries Agency does not produce organic matter by photosynthesis, and does not produce vertical mixing in winter. Deep sea water is defined as deep water. These deep sea waters are rich in inorganic nutrients necessary for life activities, rich in nutrients, cleanliness free from chemical contamination, low temperature stability with little temperature change, It has characteristics such as maturation matured over a long period of time at a pressure of 20 atm and higher, including fisheries (aquaculture), energy (cooling), product fields (food, salt, alcohol, bottled water and cosmetics) and medical It is widely used in the field (atopic skin treatment) and the like. Aging of deep sea water means that deep sea water is “very fine” and “soft” compared to surface water, which means that deep water is denser due to low temperatures and high pressures, and fine granular minerals can It is dissolved in. In addition, since deep sea water is not contaminated with artificial substances and photosynthetic action does not occur, there is a characteristic that there is little organic material, pathogens and bacteria to be decomposed and is very clean. In particular, the deep ocean water contains four minerals such as magnesium, calcium, potassium, and sodium, as well as various mineral components such as zinc, selenium, manganese, and essential trace elements.

The deep sea water that can be used in the composition of the present invention includes raw water, which is deep sea water as it is taken out, concentrated water in which the ionic components of the raw water are concentrated, permeated water in which the ionic components are reduced from the raw water, and mixtures thereof Etc. can be used. The deep sea water includes sodium ions (Na ⁺ ), potassium ions (K ⁺ ), calcium ions (Ca ²⁺ ), magnesium ions (Mg ²⁺ ), boron ions (B ³⁺ ), chlorine ions (Cl ⁻ ), It contains ionic components such as carbonate ions (CO ₃ ^2- ) and sulfate ions (SO ₄ ^2- ), and typically contains 10500 mg of Na ⁺ , 1350 mg of Mg ²⁺ , and 400 mg of Ca ^{2+ in} 1 L of raw water. It contains 380 mg of K ⁺ , and 4.6 mg of B ³⁺ . The concentrated water and permeated water are produced while removing sodium chloride from raw water by conventional methods such as reverse osmosis and electrodialysis. Usually, the concentrated water is 18,000 ~ 28,000mg Na ⁺ component, 1,900 ~ 2,800mg Mg ²⁺ component, 600 ~ 740mg Ca ²⁺ component, 400 ~ 700mg K ⁺ component per 1 liter of concentrated water (liter) , 3 to 9 mg of B ³⁺ component, the permeate is per 1 liter (liter) of permeate, 3 to 300 mg of Na ⁺ component, 0.02 to 200 mg of Mg ²⁺ component, 0.001 to 20 mg of Ca ²⁺ components, 0.1-30 mg K ⁺ component, 0.001-0.3 mg B ³⁺ component.

As the mineral water composition of the present invention, deep sea water having reduced (desalted) sodium chloride component is used. The desalted deep sea water is obtained by treating the concentrated water by heating, concentrating, filtration, dissolving the filtrate, etc. to separate calcium salt, sodium salt, sulfate salt, potassium salt, magnesium salt, and the like, and thus obtained calcium salt. By adding at least one of sulfate, potassium salt and magnesium salt to the permeate, the sodium chloride component can be reduced and the content of other mineral components can be increased. The calcium salt may include calcium carbonate (CaCO ₃ ), calcium sulfate (CaSO ₄ ), and the like, and the sodium salt may include sodium chloride and the like, and the sulfate salt may be magnesium sulfate (MgSO ₄ ) or sulfuric acid. Potassium (K ₂ SO ₄ ), sodium sulfate (Na ₂ SO ₄ ) and the like can be exemplified, and potassium chloride (KCl) and the like as the potassium salt, magnesium chloride (MgCl ₂ ) and the like as the magnesium salt. Can be illustrated. Such a method for controlling the mineral component of deep sea water is disclosed in detail in patent application 10-2006-0054899. The amount of the mineral added to the permeate may vary depending on the characteristics of the required mineral water, but the weight ratio of Mg: Ca: K is preferably in the range of 3: 0.1-3: 0.1-3, and 3: 0.5- The range of 1.5: 0.5 to 1.5 is more preferable, and when sodium ions are included, the content of sodium ions is 1.5 times (weight ratio) or less, preferably 0.05 to 1.5 times (weight ratio), more preferably, magnesium content. 0.2 to 1 times, most preferably 0.2 to 0.5 times. Here, if the ratio of the mineral component is out of the above range, there is a problem that the antibacterial activity of the mineral water is lowered, or the manufacturing cost of the mineral water increases. The increased content of mineral components, for example, sulfate ions, chlorine ions, calcium ions, etc., sterilize and disinfect bacteria and microorganisms, or bind to specific amino acids to inhibit the prolongation of viruses or bacteria, or to prevent their reproduction. Do it.

The hardness of the deep sea water is preferably 80 to 3000, more preferably 200 to 1500, and most preferably 400 to 1100. Here, hardness is the content of the cation (magnesium and calcium) of mineral water, and it calculates by the formula of hardness = (magnesium x 4.116) + (calcium x 2.5). If the hardness of the deep sea water is too low, there is a fear that the antimicrobial effect of the composition is lowered. If the hardness is too high, there is a problem such that the salty taste of sodium chloride becomes stronger without particular benefit. The hardness of the deep sea water may be adjusted by appropriately mixing two or more selected from the group consisting of the raw water, the concentrated water, the permeated water, and the purified water, or by adjusting the content of minerals added to the permeated water. For example, the mineral water composition may be prepared by mixing raw water: filtrate: concentrated water in a volume ratio of 1: 0.2-2: 0.2-3, preferably 1: 0.5-2: 0.5-3. In addition, as the purified water, distilled water, tap water, bottled water, and the like may be used without particular limitation.

The antimicrobial mineral water composition according to the present invention is used as sterilization and washing water for fresh foods, food materials, etc., which not only contributes to the improvement of hygiene, but also is useful as a preparation water or raw material for a product requiring antibacterial function such as food, beverage, cosmetics and medicines. Do.

Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples. The following examples illustrate the present invention and the present invention is not limited by the following examples.

Example 1 Preparation of Antimicrobial Mineral Water Composition and Hardness Measurement

end. Pretreatment and Deionization Process of Deep Sea Water

The deep sea water containing the minerals of Table 1 was micro-filtered with a micro filter (material: Polytetrafluoroethylene (PTFE), manufactured by Saehan Co., Ltd.) having pores of 0.2 to 1.0 μm, followed by a first reverse osmosis system ( Dow Chemical Company, FILMTEC, SW30-4021, yield: 0.5), and separated into first concentrated water and first permeate water. The first permeate was transferred to a second reverse osmosis system (Saehan Co., Ltd., RE-2521-TE, yield: 0.85) and separated into a second concentrated water and a second permeated water. The separated second concentrated water was recycled back into the deep sea water and mixed, and the process of passing the first reverse osmosis system was continuously performed, and the mineral water using the first concentrated water and the second permeated water was used. Was prepared. The mineral composition of the deep ocean water, the first permeate, the second permeate, and the first concentrated water is shown in Table 1 below.

Mineral (mg / l) Sea water (raw water) Primary Permeate Secondary permeate Primary concentrated water Na 14000 284.1 5.7 21700 Mg 1310 26.58 0.53 2031 Ca 410 8.32 0.17 636 K 430 8.7 0.17 666.5 B 4.2 0.4 0.04 6.46

I. Extraction of minerals

1 L of the first concentrated water was heated at 70 ° C. until the specific gravity of Bumedo was 24 ° Be, to precipitate 1852 mg of calcium sulfate (CaSO ₄ ). After the mineral salt was precipitated, the filtrate was heated at 70 ° C until the specific gravity of Bomedo became 30 ° Be, to precipitate 41374 mg of sodium chloride (NaCl) crystals. After the sodium chloride (NaCl) crystals were precipitated, the filtrate was heated at 70 ° C until the specific gravity of Bomedo became 32 ° Be, to precipitate 2427 mg of magnesium sulfate (MgSO ₄ ). Thereafter, the filtrate was heated at 50 ° C until the specific gravity of Bumedo was 34 ° Be, to precipitate 10700 mg of crystals of potassium chloride, magnesium chloride, dihydrate (KCl, MgCl ₂ , 2H ₂ O). Next, the precipitated potassium chloride magnesium chloride dihydrate (KCl MgCl _2v 2H ₂ O) crystals were washed with 0.5 L of sterilized distilled water to separate 372.3 mg of potassium chloride (KCl) crystals. After separating the potassium chloride (KCl), the filtrate was heated until the specific gravity of the Bumedo 3737Be, to precipitate 5432.1mg of magnesium chloride dihydrate (MgCl ₂ · 2H ₂ O) crystals. The Bomedo specific gravity was measured by a Bomedo hydrometer (Daekwang meter, Heavy Baume hydrometer).

All. Preparation of Mineral Water Composition and Measurement of Hardness

에 따라, 미네랄수에 함유된 탄산칼슘의 양으로서 경도(㎎/ℓ)를 구하였다. 측정된 경도에 따른 해양 심층수의 주성분을 하기 표 2에 타내었다. Dissolve calcium sulfate (CaSO ₄ ), magnesium sulfate (MgSO ₄ ), potassium chloride (KCl), and magnesium chloride (MgCl ₂ ) separated in the “b” step in the second permeated water prepared in step “a”. And stirred to prepare a composition of mineral content and hardness (100, 500 and 1000) shown in Table 2 below. At this time, the hardness was measured by the following method. First, 10 g of potassium cyanide was dissolved in water to prepare 100 ml of potassium cyanide solution, about 2.1 g of magnesium chloride (hexahydrate) was dissolved in water to prepare 1 L of magnesium chloride solution (0.01 M), and 67.5 g of ammonium chloride was prepared. 1 L of ammonia buffer was prepared by dissolving in 570 ml of ammonia water. In addition, 0.5 g of eryochrome black T and 4.5 g of hydrochloric acid hydroxylamine were dissolved in ethanol to make 100 ml of an EBT (eriochrome black T indicator), and ethylenediamine tetrasodium acetate (dihydrate) at 80 ° C for 5 hours. After drying and cooling in a desiccator, 3.722 g of water was dissolved in water to 1 liter, and then placed in a brown bottle, containing EDTA solution (0.1 M, 1 ml of which corresponds to 1 mg of calcium carbonate). Prepared. Add 100 ml of mineral water (100 ml of water in mineral water so that calcium carbonate is 10 mg or less) into a Erlenmeyer flask, add a few drops of potassium cyanide solution, 1 ml of magnesium chloride solution, and 2 ml of ammonia buffer. Put in. Next, a few drops of EBT solution was used as an indicator, and titrated with EDTA solution (0.01M) until the number of minerals became reddish purple to blue. From this, ml of a consumed EDTA solution (0.01M)

The hardness (mg / L) was determined as the amount of calcium carbonate contained in the mineral water. The main components of the deep sea water according to the measured hardness are shown in Table 2 below.

ingredient Hardness (mg / L): 100 Hardness (mg / L): 500 Hardness (mg / L): 1000 K 5.79 29.48 58.71 Na 18.43 91.42 182.43 Mg 20.65 102.58 205.14 Ca 6.21 30.92 61.83 Cl 21.07 106.18 211.15 SO4 3.52 17.88 35.77 B 0.10 0.52 0.80 Cu 0.14 0.61 0.88 Zn 0.26 1.98 2.41 Total content 100.26 381.57 759.12

Inoculated with the cultured mesophilic bacteria (mesophiles: 25 ~ 45 ℃) in the mineral water (hardness 100, 500 and 1000) and the control group (water purification, Comparative Example) prepared in Example 1, rotating at 120 rpm at 37 ℃ The mesophilic bacteria were cultured. The growth change of mesophilic bacteria over time was measured and shown in Table 3 (unit: CFU / mL).

Mineral water of hardness 100 Mineral water of hardness 500 Mineral water of hardness 1000 Control Early 2.0 x 10 ⁶ 2.0 x10 ⁶ 2.0 x 10 ⁶ 2.0 x 10 ⁶ 7 days later 1.9 x 10 ⁴ 1.4 x10 ⁴ 1.2 x 10 ⁴ 3.4 x 10 ⁴ 15 days later 2.7 x 10 ² 2.1 x10 ² 3.0 x 10 ² 3.4 x 10 ³ After 20 days 2.3 x10 ² 1.9 x 10 ² 2.7 x 10 ² 2.1 x 10 ³ 22 days later 2.0 x10 ² 1.0 x10 ² 2.0 x10 ² 6.0 x 10 ² 25 days later 1.3 x 10 ² 27 0 5.8 x 10 ² 30 days later 1.2 x 10 ² 0 0 4.7 x 10 ²

From Table 3, after one week of culture, the number of viable microorganisms decreased in mineral water compared to the control (water), and as time goes by, and the higher the mineral content, it can be seen that the microbial reduction rate is further increased. Therefore, it can be seen that the mineral water of the present invention has a significant microbial growth inhibitory effect.

Experimental Example 2 Measurement of Pathogenic Bacteria According to Storage Period

Instead of mesophilic bacteria, four pathogenic bacteria ( Staphylococcus aureus , E. coli , Salmonella typhimurium and Pseudomonas aeruginosa ) were used, except that the growth changes of the pathogenic bacteria were measured in the same manner as in Experimental Example 1, and the results were as follows. Tables 4 to 7 (unit: CFU / mL) are shown respectively.

Staphylococcus aureus Mineral water of hardness 100 Mineral water of hardness 500 Mineral water of hardness 1000 Control Early 2.0 x 10 ⁶ 2.0 x 10 ⁶ 2.0 x 10 ⁶ 2.0 x 10 ⁶ 7 days later 4.5 x 10 ⁴ 3.3 x 10 ⁴ 2.2 x 10 ⁴ 6.5 x 10 ⁴ 15 days later 6.8 x 10 ² 2.3 x 10 ² 1.6 x 10 ² 8.9 x 10 ³ After 20 days 4.1 x 10 ² 1.2 x 10 ² 48 7.7 x 10 ³ 25 days later 27 0 0 7.1 x 10 ² 30 days later 0 0 0 5.0 x 10 ²

E. coli Mineral water of hardness 100 Mineral water of hardness 500 Mineral water of hardness 1000 Control Early 2.0 x 10 ⁶ 2.0 x 10 ⁶ 2.0 x 10 ⁶ 2.0 x 10 ⁶ 7 days later 5.6 x 10 ⁴ 4.8 x 10 ⁴ 2.3 x 10 ⁴ 6.5 x 10 ⁴ 15 days later 5.9 x 10 ² 2.2 x 10 ² 1.1 x 10 ² 4.3 x 10 ⁴ After 20 days 3.4 x 10 ² 31 0 8.1 x 10 ³ 25 days later 24 0 0 5.8 x 10 ² 30 days later 0 0 0 5.5 x 10 ²

Salmonella typhimurium Mineral water of hardness 100 Mineral water of hardness 500 Mineral water of hardness 1000 Control Early 2.0 x 10 ⁶ 2.0 x 10 ⁶ 2.0 x 10 ⁶ 2.0 x 10 ⁶ 7 days later 8.0 x 10 ⁴ 5.4 x 10 ⁴ 4.4 x 10 ⁴ 7.3 x 10 ⁴ 15 days later 6.5 x 10 ² 2.2 x 10 ² 1.8 x 10 ² 6.9 x 10 ⁴ After 20 days 87 80 0 5.9 x 10 ³ 25 days later 20 0 0 4.0 x 10 ² 30 days later 0 0 0 3.2 x 10 ²

Pseudomonas aeruginosa Mineral water of hardness 100 Mineral water of hardness 500 Mineral water of hardness 1000 Control Early 2.0 x 10 ⁶ 2.0 x 10 ⁶ 2.0 x 10 ⁶ 2.0 x 10 ⁶ 7 days later 6.5 x 10 ⁴ 5.1 x 10 ⁴ 3.2 x 10 ⁴ 7.7 x 10 ⁴ 15 days later 8.3 x 10 ² 3.9 x 10 ² 1.6 x 10 ² 6.8 x 10 ⁴ After 20 days 3.5 x 10 ² 47 0 9.0 x 10 ³ 25 days later 74 0 0 8.5 x 10 ² 30 days later 0 0 0 5.1 10 ²

From Tables 4 to 7, it can be seen that the mineral water of the present invention has a significant microbial growth inhibitory effect against pathogenic bacteria.

Experimental Example 3 Antimicrobial Effect Measurement by Paper Disc Method

For paper Gram positive bacteria Staphylococcus aureus (37 ° C), Gram-negative bacterium Escherichia coli (37 ° C), Salmonella typhimurium (30 ° C), and Pseudomonas aeruginosa (37 ° C) using paper disc agar diffusion method. , The antimicrobial activity of mineral water was measured. Specifically, the bacteria were sterilized by platinum in sterilized tryptic soybean broth, and shaken at each culture temperature, and the OD (optical density) value was adjusted to 0.1. 50 μl of mineral water for each hardness is injected into a paper disc (paper disc, φ 8 mm, Whatman), dried, and attached to the prepared agar medium. Each medium was incubated in the incubator, the diameter of the clear zone (clear zone) showing the antimicrobial was determined by measuring the antimicrobial, the results are shown in Table 8.

Staphylococcus E. coli Salmonella Pseudomonas Control - - - - Mineral water of hardness 100 + + + + Mineral water of hardness 300 + + + + Mineral water of hardness 500 + + + + Mineral water of hardness 1000 + ++ ++ ++

In Table 8, "-" indicates no growth inhibition (no inhibition (clear zone diameter 8 mm or less)), "+" indicates slight growth inhibition (clear inhibition (clear zone diameter 8-9 mm)), and "++" "Represents moderate inhibition (clear zone diameter 10-11 mm) and" +++ "represents heavy inhibition (clear zone diameter 12 mm). From Table 8, it can be seen that the mineral water of the present invention has a significant microbial growth inhibitory effect against pathogenic bacteria.

Claims (7)

The antimicrobial mineral water composition containing Mg, Ca, and K as a mineral component. The antimicrobial mineral water composition according to claim 1, comprising deep sea water as an active ingredient and comprising a mineral such that the weight ratio of Mg: Ca: K is in the range of 3: 0.1-3: 0.1-3. The antimicrobial mineral water composition of claim 1, wherein the content of sodium ions in the mineral water composition is 0.05 to 1.5 times (weight ratio) of the magnesium ion content. The antimicrobial mineral water composition according to claim 3, wherein the content of sodium ions in the mineral water composition is 0.2 to 1 times (weight ratio) of the content of magnesium ions. The antimicrobial mineral water composition of claim 1, wherein the hardness of the mineral water composition is 80 to 3000. Preparing from the deep sea water, concentrated water in which the ionic components of the raw water are concentrated, and permeate having reduced ionic components from the raw water; And From the concentrated water, calcium salts, sodium salts, sulfates, potassium salts and magnesium salts are obtained by separating, and by adding one or more of the obtained calcium salts, sulfates, potassium salts and magnesium salts to the permeate, the sodium chloride component is reduced. And, the method of producing an antimicrobial mineral water composition comprising the step of increasing the content of other mineral components. According to claim 6, wherein the concentrated water is 18,000 ~ 28,000mg of Na ⁺ component, 1,900 ~ 2,800mg Mg ²⁺ component, 600 ~ 740mg Ca ²⁺ component, 400 ~ 700mg K per 1 liter of concentrated water ⁺ Component, 3 to 9 mg of B ³⁺ component, the permeate is per 1 liter of permeate, 3 to 300 mg of Na ⁺ component, 0.02 to 200 mg of Mg ²⁺ component, 0.001 to 20 mg of Ca ² components ^+, K ⁺ component of 0.1 ~ 30mg, be antimicrobial mineral comprises a component of the B ³⁺ 0.001 ~ 0.3mg composition.