CN107286226A - The preparation method of high clarity and the high-titer nisin aqueous solution - Google Patents
The preparation method of high clarity and the high-titer nisin aqueous solution Download PDFInfo
- Publication number
- CN107286226A CN107286226A CN201710507771.3A CN201710507771A CN107286226A CN 107286226 A CN107286226 A CN 107286226A CN 201710507771 A CN201710507771 A CN 201710507771A CN 107286226 A CN107286226 A CN 107286226A
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- Prior art keywords
- aqueous solution
- nisin
- potency
- salt
- titer
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/42—Preservation of non-alcoholic beverages
- A23L2/44—Preservation of non-alcoholic beverages by adding preservatives
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Gastroenterology & Hepatology (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nutrition Science (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention discloses a kind of high clarity and the preparation method of the high-titer nisin aqueous solution, comprise the following steps：（1）The nisin powder for taking potency to be more than 35000 IU/mg is put into the aqueous solution for being made into that potency is 50000 IU/ml in distilled water；（2）The above-mentioned aqueous solution is subjected to hyperfiltration treatment by 2000D milipore filters, clear water solution is obtained；（3）Above-mentioned clear water solution is sterilized at 80 DEG C and carries out being filtrated to get the nisin aqueous solution by 0.2 μm of miillpore filter after 10min.The present invention is using high-purity nisin as raw material, through ultrafiltration membrance filter, is made after the concentration of reverse osmosis membrane desalination, nisin aqueous solution potency is more than 1000000 IU/mL, place 1 year it is not muddy, can be widely used for the anti-corrosion of beverage, health products and green product etc..
Description
Technical field
The present invention relates to a kind of preparation method of chemicals, more particularly to a kind of high clarity and high-titer streptococcus lactis
The preparation method of the plain aqueous solution.
Background technology
Nisin is also known as nisin, and also known as nisin is a kind of natural, efficient food additives, energy
Suppress gram-positive bacteria, be widely used in various food, the anti-corrosion of beverage etc..The food antiseptic application of nisin is
More than more than 10 years.
Most of commercially available nisin is its compounding product with sodium chloride, and potency is relatively low, is only about 1000 IU/mg, pure
Degree about 2.5%, it is more muddy, and can not dissolved clarification.
The content of the invention
The purpose of the present invention is for low and not limpid enough the technical problem of the current nisin aqueous solution potency of solution.
In order to solve the above-mentioned technical problem, the present invention provides a kind of high clarity and the high-titer nisin aqueous solution
Preparation method, it is characterised in that comprise the following steps：
（1）The nisin powder for taking potency to be more than 35000 IU/mg, which is put into distilled water, is made into potency for 50000 IU/
The ml aqueous solution；
（2）The above-mentioned aqueous solution is subjected to hyperfiltration treatment by 2000D milipore filters, clear water solution is obtained；
（3）Above-mentioned clear water solution is sterilized at 80 DEG C and carries out being filtrated to get breast by 0.2 μm of miillpore filter after 10min
The acid streptococci element aqueous solution, makes its sediment without macroscopic.
Further, the step（3）Also comprise the following steps below（4）：
（4）Salt in the nisin aqueous solution is removed by milipore filter, then carries out being concentrated to give less salt lactic acid chain
The coccus element aqueous solution, the mass content of salt therein is less than 3%, and the salt includes sodium chloride, potassium chloride, sodium sulphate and potassium sulfate.
Further, 3≤pH value≤4 of the less salt nisin aqueous solution.
Further, the step（4）Also comprise the following steps below（5）：
（5）The less salt nisin aqueous solution is stood 1 year at 25 DEG C, makes its sediment without macroscopic.
Further, the less salt nisin aqueous solution has high clarity, and its 354 nm light transmittance >=
37.1%。
Further, the less salt nisin aqueous solution has high-titer, the IU/ml of its potency >=1000000.
The present invention is using high-purity nisin as raw material, through ultrafiltration membrance filter, is made after the concentration of reverse osmosis membrane desalination, nisin water
Solution potency be more than 1000000 IU/mL, place 1 year it is not muddy, can be widely used for beverage, health products and green product etc.
Anti-corrosion.
Aqueous solution potency produced by the present invention is high, it is possible to decrease cost of transportation；Impurity is few, is more than 35000 IU/ using potency
Mg nisin powder is prepared through desalination, impurity, other metabolites that such as some fermentations are produced, seldom；As clear as crystal, room temperature is put
More than 1 year is put without flocculent deposit, the anti-corrosion available for beverages such as fruit juice；Low salinity, does not influence flavour of food products.
Embodiment
Presently in connection with embodiment, the present invention is further detailed explanation, and application of the invention is not limited to following
Embodiment, protection scope of the present invention is fallen within to any formal accommodation that the present invention is done.
The high clarity of the present invention and the preparation method of the high-titer nisin aqueous solution, comprise the following steps：
（1）The nisin powder for taking potency to be more than 35000 IU/mg, which is put into distilled water, is made into potency for 50000 IU/
The ml aqueous solution；
（2）The above-mentioned aqueous solution is subjected to hyperfiltration treatment by 2000D milipore filters, clear water solution is obtained；It should be noted that
During trial-production, select the milipore filter of tri- kinds of specifications of 3000D, 2000D and 1000D to test one by one, it is found that 3000D milipore filters have race
Material, 2000D milipore filters are limpid to expect without race, muddy after 1000D milipore filters, so final choice 2000D milipore filters, D therein
For atomic mass unit dalton；
（3）Above-mentioned clear water solution is sterilized at 80 DEG C and carries out being filtrated to get breast by 0.2 μm of miillpore filter after 10min
The acid streptococci element aqueous solution, makes its sediment without macroscopic.
Preferably, the step（3）Also comprise the following steps below（4）：
（4）Salt in the nisin aqueous solution is removed by milipore filter, then carries out being concentrated to give less salt lactic acid chain
The coccus element aqueous solution, the mass content of salt therein is less than 3%, and the salt includes sodium chloride, potassium chloride, sodium sulphate and potassium sulfate.
Preferably, 3≤pH value≤4 of the less salt nisin aqueous solution.
Preferably, the step（4）Also comprise the following steps below（5）：
（5）The less salt nisin aqueous solution is stood 1 year at 25 DEG C, makes its sediment without macroscopic.
Preferably, the less salt nisin aqueous solution has high clarity, its 354 nm light transmittance >=37.1%.
Preferably, the less salt nisin aqueous solution has high-titer, the IU/ml of its potency >=1000000.
It is high by aqueous solution potency made from this method, it is possible to decrease cost of transportation；Impurity is few, is more than 35000 using potency
IU/mg nisin powder is prepared through desalination, impurity, other metabolites that such as some fermentations are produced, seldom；It is as clear as crystal, room temperature
More than 1 year is placed without flocculent deposit, the anti-corrosion available for beverages such as fruit juice；Low salinity, does not influence flavour of food products.
Using the above-mentioned desirable embodiment according to the present invention as enlightenment, by above-mentioned description, relevant staff is complete
Various changes and amendments can be carried out without departing from the scope of the technological thought of the present invention' entirely.The technology of this invention
Property scope is not limited to the content on specification, it is necessary to its technical scope is determined according to right.
Claims (6)
1. the preparation method of high clarity and the high-titer nisin aqueous solution, it is characterised in that comprise the following steps：
（1）The nisin powder for taking potency to be more than 35000 IU/mg, which is put into distilled water, is made into potency for 50000 IU/
The ml aqueous solution；
（2）The above-mentioned aqueous solution is subjected to hyperfiltration treatment by 2000D milipore filters, clear water solution is obtained；
（3）Above-mentioned clear water solution is sterilized at 80 DEG C and carries out being filtrated to get breast by 0.2 μm of miillpore filter after 10min
The acid streptococci element aqueous solution, makes its sediment without macroscopic.
2. according to the method described in claim 1, it is characterised in that the step（3）Also comprise the following steps below（4）：
（4）Salt in the nisin aqueous solution is removed by milipore filter, then carries out being concentrated to give less salt lactic acid chain
The coccus element aqueous solution, the mass content of salt therein is less than 3%, and the salt includes sodium chloride, potassium chloride, sodium sulphate and potassium sulfate.
3. method according to claim 2, it is characterised in that 3≤pH value of the less salt nisin aqueous solution
≤4。
4. method according to claim 2, it is characterised in that the step（4）Also comprise the following steps below（5）：
（5）The less salt nisin aqueous solution is stood 1 year at 25 DEG C, makes its sediment without macroscopic.
5. method according to claim 4, it is characterised in that the less salt nisin aqueous solution has high limpid
Degree, its 354 nm light transmittance >=37.1%.
6. the method according to claim any one of 1-5, it is characterised in that the less salt nisin aqueous solution tool
There is high-titer, the IU/ml of its potency >=1000000.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710507771.3A CN107286226A (en) | 2017-06-28 | 2017-06-28 | The preparation method of high clarity and the high-titer nisin aqueous solution |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710507771.3A CN107286226A (en) | 2017-06-28 | 2017-06-28 | The preparation method of high clarity and the high-titer nisin aqueous solution |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111995667A (en) * | 2019-05-27 | 2020-11-27 | 浙江工业大学 | Preparation method and application of nisin sorbate |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101066994A (en) * | 2007-04-20 | 2007-11-07 | 上海奇泓生物科技有限公司 | Process of extracting lactic streptocin from fermented liquid |
| CN102199200A (en) * | 2010-03-22 | 2011-09-28 | 上海医药工业研究院 | Method for preparing high-purity nisin |
| CN102304172A (en) * | 2011-08-12 | 2012-01-04 | 郑州奇泓生物科技有限公司 | Nisin extraction method |
| CN105440113A (en) * | 2014-09-30 | 2016-03-30 | 李一凡 | Extraction method for nisin |
| WO2016177650A1 (en) * | 2015-05-07 | 2016-11-10 | Bayer Technology Services Gmbh | Modular system and method for continuously producing and/or preparing a product in a disinfected manner |
| CN106565831A (en) * | 2016-09-20 | 2017-04-19 | 浙江新银象生物工程有限公司 | Method for preparing high-purity nisin through carrying out separation by using integrated membrane technology |
| CN106749564A (en) * | 2016-12-07 | 2017-05-31 | 浙江新银象生物工程有限公司 | Membrane technology combination crystallisation separates the method for preparing high-purity N isin |
-
2017
- 2017-06-28 CN CN201710507771.3A patent/CN107286226A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101066994A (en) * | 2007-04-20 | 2007-11-07 | 上海奇泓生物科技有限公司 | Process of extracting lactic streptocin from fermented liquid |
| CN102199200A (en) * | 2010-03-22 | 2011-09-28 | 上海医药工业研究院 | Method for preparing high-purity nisin |
| CN102304172A (en) * | 2011-08-12 | 2012-01-04 | 郑州奇泓生物科技有限公司 | Nisin extraction method |
| CN105440113A (en) * | 2014-09-30 | 2016-03-30 | 李一凡 | Extraction method for nisin |
| WO2016177650A1 (en) * | 2015-05-07 | 2016-11-10 | Bayer Technology Services Gmbh | Modular system and method for continuously producing and/or preparing a product in a disinfected manner |
| CN106565831A (en) * | 2016-09-20 | 2017-04-19 | 浙江新银象生物工程有限公司 | Method for preparing high-purity nisin through carrying out separation by using integrated membrane technology |
| CN106749564A (en) * | 2016-12-07 | 2017-05-31 | 浙江新银象生物工程有限公司 | Membrane technology combination crystallisation separates the method for preparing high-purity N isin |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111995667A (en) * | 2019-05-27 | 2020-11-27 | 浙江工业大学 | Preparation method and application of nisin sorbate |
| CN111995667B (en) * | 2019-05-27 | 2022-10-11 | 浙江工业大学 | Preparation method and application of nisin sorbate |
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Application publication date: 20171024 |