KR101533195B1 - Wet Tissue composition for the Natural preservative - Google Patents

Abstract

Disclosed is a natural antiseptic composition which is made of natural materials and provides an antiseptic effect for cosmetics. To this end, the wet tissue composition according to the present invention exhibits reduced skin irritation, and is manufactured by using natural antiseptics and other wet tissue raw materials. The natural antiseptics is obtained by fermenting a mixture including 10 to 40 parts by weight of a Codonopsis Lanceolata extract, 10 to 30 parts by weight of a Citrus unshiu extract, and 15 to 40 parts by weight of a pine needle extract at a room temperature for 5 to 7 days, and aging a vegetable fermentation liquid which is recovered by solid-liquid separation at a room temperature for 5 to 7 days. Accordingly, the natural antiseptic composition according to the present invention consists of natural materials, thereby having no side effects to a human body. Thus, the natural antiseptic composition is very appropriate for antiseptic treatment of wet tissues directly come in contact with a human body, and has an excellent antiseptic effect for wet tissues that are easily degenerated.

Description

[0001] The present invention relates to a safe wet tissue composition using a natural preservative,

The present invention relates to a wet tissue composition, and more particularly, to a wet tissue composition using a natural herbicide containing a complex herbal extract and a stimulant to produce a skin-safe wipes containing a large amount of herbal extract and a stimulant.

In recent years, the income has increased, the cleanliness has been emphasized, and the use of wipes has been increasing as interest in the appearance has increased. Such a wet tissue is a product that comes into direct contact with a human body. Since the wet tissue is used for a relatively long period of time after the package is opened, and the characteristics of the product tend to change, it is essential to use a preservative. In particular, in recent years, there has been an increasing tendency to place importance on health and wellness, and in order to meet such demands, various types of wet tissues are manufactured using natural materials.

Accordingly, conventionally, a certain amount of a synthetic preservative, which is a chemical compound synthesized, is added to the wet tissue to prevent the wet tissue from being deteriorated or corrupted. Although these preservatives are excellent chemical preservatives for wet tissues, they are chemical products made by synthesizing chemical substances. Therefore, it affects the skin of users of wet tissues, and it is naturally arranged with the demands and social trends of users of wet tissues that emphasize health and well- .

Accordingly, it is a first object of the present invention to provide a pharmaceutical composition of a natural preservative composition which is suitable for a product that is in direct contact with a human body, such as a wet tissue, because it has no side effects on the human body, The present invention provides a wet tissue composition that is suitable for a product that is in direct contact with a human body and has excellent preservative effect on a wet tissue because it has no side effects on the human body because it is made of a material and the third object is to reduce the irritation of the skin To constitute a wet tissue composition

In order to accomplish the first object of the present invention, in one embodiment of the present invention, 10% to 40% by weight of roots extract, 10% to 30% by weight of citrus extract, And 15% to 40% by weight of pine needle extract is fermented at room temperature for 5 days to 7 days and recovered by solid / liquid separation is aged at room temperature for 5 days to 7 days to provide a natural preservative composition .

In order to achieve the second object of the present invention, in an embodiment of the present invention, 10% to 40% by weight of roots extract; 10% to 30% by weight citrus extract; And 15 to 40% by weight of pine needle extract, and fermenting the mixture for 5 to 7 days, and recovering the mixture by solid / liquid separation; aging the plant fermentation broth at room temperature for 5 days to 7 days; And a pharmaceutically acceptable carrier. ≪ Desc / Clms Page number 2 >

Since the natural preservative composition according to the present invention is made only of natural materials and has no adverse effect on human body, it is very suitable for preserving wet tissues in direct contact with human body, and has an effect of excelling in antiseptic effect against easily deteriorated wipes.

In order to achieve the third object of the present invention, in order to constitute a wet tissue composition for relieving irritation of the skin in a wet tissue used for skin, the ratio of 15% to 40% by weight of glycerin and allantoin, By weight of the wipes.

One embodiment of the natural preservative composition of the present invention will be described in detail.

First, a mixture comprising 10 to 40 parts by weight of roeae extract, 10 to 30 parts by weight of citrus extract, and 15 to 40 parts by weight of pine leaf extract is formed.

Codonopsis Lanceolata is a perennial plant that is deeply rooted in the root, rooted similar to ginseng and bellflower, and its roots are well known for its medicinal and food uses. In autumn, the dried root is called "samsam", while the one is used as a tonic, and it is prescribe to the cough, the ghost and the cough due to the waste heat. Doduck has a large amount of saponin like ginseng, and has a relatively high content of riboflavin, which is known to be effective for anti-cancer effect and prevention of adult diseases and has been widely used for dissolution, antipyretic, and antidote.

In the present invention, roots of the medicinal herb, which is a medicinal herb, are crushed to form crumbs, crushing the crumbs of the crumbs to obtain a Crassulaceae extract, and 10 to 40 parts by weight of Crassulaceae extract to form a mixture.

If the content of roots extract is less than 10 parts by weight, the inherent pharmacological action of rosewood is deteriorated. If the roots extract is more than 40 parts by weight, the content of rosewood extract is relatively high, Is inhibited.

In addition, citrus is cultivated only in Jeju and southern part of the country because it is the most northern part of citrus plantation in the world. Citrus cultivated varieties of citrus are the cold-tolerant tangerine tree .

Citrus fruits from Jeju are good in taste and flavor and contain various vitamins, especially vitamins A and C. In addition, it contains many physiologically active ingredients such as pectin, various essential oils, and hesperidin Hesperidin. It is one of the most used fruits for reproductive health in Korea. have. However, due to the imbalance of demand due to excessive production, there is a difficulty in farmers producing citrus fruits. In this study, a new study on the use and demand of citrus fruits from Jeju has been conducted actively (Korean J. Food Sci. Technol Vol. 34, No. 1, pp 132-135, 2002). However, studies on pectin, hesperidin and naringin have been carried out in Korea. However, studies on essential oil components are insufficient.

According to Korean Patent Laid-Open No. 10-2004-0082846, an essential oil component having antimicrobial activity against Trichophyton rubrum and Trichophyton mentagrophytes, which are major pathogens causing skin diseases such as athlete's foot, is called citrus unshiu ), Which is effectively used for the treatment and prevention of athlete's foot, and has no side effects

In the present invention, the citrus peel is pulverized to form a citrus crushed material. The citrus crushed material is compressed to obtain a liquid citrus extract. The citrus extract is contained in an amount of 10 to 30 parts by weight to form a mixture.

If the content of the citrus extract contained in the mixture is less than 10 parts by weight, the pharmacological action inherent to the citrus decreases. When the content of the citrus extract exceeds 30 parts by weight, the content of the citrus extract is relatively high, Is inhibited.

Pine needles have been used as medicines or health foods for a long time or as a folk remedy, and they are recorded as longevity foods in Oriental medicine books such as Dongbokgop. According to Dongyubogam, Pine Needles is known to have a therapeutic effect in gastrointestinal diseases, stroke, hypertension, neuralgia and asthma. This pharmacological action of pine needles is made up of ingredients such as essential oil, protein, vitamin A, vitamin C, vitamin K and flavonoid in pine leaves. In particular, pine leaves have terpene components such as alpha-pinene, beta-pinene, and beta-palandrene, which are volatile components that fly into the air. When terpenes are absorbed into the body, they stimulate blood vessels to make blood circulate, To remove parasites and germs. Especially, Terpen is a substance that gives a refreshing sensation, and since it emits a peculiar smell of pine needles, pine leaves have been used as beverage materials such as pine needles.

The pine needle, which is a raw material of a natural preservative composition, is a softwood leaf, and has inhibitory effect on growth and sterilization action against various fungi. Therefore, it is useful as a fungicide and a natural food preservative.

In the present invention, the pine needle leaf is pulverized to form a pine needle leaf, and the pine needle leaf material is pressed to obtain a liquid pine leaf extract. The pine needle extract is contained in an amount of 15 to 40 parts by weight to form a mixture.

If the content of the pine leaf extract contained in the mixture is less than 15 parts by weight, the growth inhibition and bactericidal action against various bacteria of the natural preservative composition composed of the above-mentioned mixture is reduced. If the pine leaf extract content exceeds 40 parts by weight, Relatively overdose inhibits the synergistic effect with rosemary extract and citrus extract.

The mixture consisting of the above components and content is allowed to stand at room temperature for 5 to 7 days to ferment. During the fermentation of the mixture which is the intermediate, the constituents, roe extract, citrus extract and pine leaf extract, synergize with each other, maximizing the antibacterial function and various pharmacological actions of the mixture, and precipitating the solid content of the mixture . When the extract is mixed with a certain amount of the pine extract, citrus extract and pine leaf extract, the extracts synergize with each other to form a mixture having an excellent preservative function, and the mixture is subjected to fermentation, solid / liquid separation and aging A natural preservative composition is formed. That is, the natural preservative composition of the present invention synergizes with each other only when the extracts of Rootgrass, Citrus, and pine leaf are all included, thereby providing excellent preservative function.

Since the natural preservative composition according to the present invention is composed of only natural materials and has no adverse effect on the human body, it is very suitable for preserving the wet tissue which is in direct contact with the human body.

In addition, the natural preservative composition exhibits an excellent preservative function due to mutual synergism between the raw materials dodok, citrus, and pine needles, and therefore, the preservative effect on the wet tissue, which is likely to be deteriorated and decayed, is excellent

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention as defined by the following claims. It can be understood that it is possible.

Hereinafter, the present invention will be described in detail with reference to the preferred embodiments. It should be noted, however, that the present invention is not limited to the following examples.

≪ Example 1 >

1. Root of the medicinal herb is crushed to form crumbs, and the crumbs of crumbs are squeezed to obtain a liquid Linderk's extract.

2. Crust of citrus is crushed to form citrus crushed material, and the citrus crushed material is squeezed to obtain a liquid citrus extract

3. Pine needle was pulverized to form a crushed pine leaf, and the crushed pine leaf was pressed to obtain a liquid pine leaf extract.

4. 250 g of the above extract, citrus extract 200 g, and pine leaf extract 300 g were mixed in a reactor and stirred to form a mixture.

5. The mixture was allowed to stand at room temperature for 6 days for fermentation.

6. The fermented mixture was filtered to remove solids, and the liquid fermentation broth was recovered.

7. A natural preservative composition was prepared by aging the vegetable fermentation broth at room temperature for 6 days.

≪ Example 2 >

To the natural preservative composition prepared in Example 1 were added 1,000 g of purified anion, 1000 g of a dodeca liquid extract, 200 g of a citrus liquid extract, 300 g of a pine needle liquid extract, 53 g of glycerin as a humectant, glycerin as a humectant and allantoin as a stimulant emollient And a weight ratio of 100%. The nonwoven fabric is sprayed on the nonwoven fabric so that the weight of the nonwoven fabric is 3 times as much as the weight of the raw paper.

<Experimental Example 1>

The preservative effects of the natural preservative compositions prepared according to the above Examples, the fermentation broths prepared according to Comparative Examples 1 to 6, and the conventional synthetic preservative were tested. In the experiment for measuring the preservative effect, the conventional synthetic preservative, the fermentation broths of Comparative Examples 1 to 6 and the natural preservative compositions of Examples were respectively added to the wet tissue cosmetics to form samples. Staphylococcus aureus ATCC6538

7.0 × 10 ⁷ / g, and the viable cell count was measured at a certain time point for 4 weeks to compare the preservative effect.

Table 1 shows the results of the preservation effect measurement experiment.

Table 1 shows the results of simultaneous inoculation of the first strain with a sample to which the natural antiseptic composition of the Example was added, a sample to which the conventional synthetic preservative was added, a sample to which the fermentation broth of each of Comparative Examples 1 to 6 was added, And at the same time, the viable cell count was measured at the same time.

Comparison test results of preservative effect on first strain of wet wipes division Day of inoculation 3 days 1 week Two weeks Three weeks Four weeks Example 7.0 x 10 ⁷ 4.0 x 10 ³ 30 0 0 0 Synthetic preservative 7.0 x 10 ⁷ 4.0 x 10 ³ 30 0 0 0 No preservative treatment 7.0 x 10 ⁷ 7.0 x 10 ⁸ 5.0 x 10 ⁷ 5.0 x 10 ⁷ 8.0 x 10 ⁷ 9.0 x 10 ⁷

In Table 1, the antiseptic function of the natural antiseptic composition according to the present invention was far superior to that of the conventional synthetic preservative and antiseptic treatment.

In conclusion, according to the result of measurement of the preservative effect over a plurality of times including the above-mentioned experimental examples, it has been proved that the natural preservative composition of the present invention has the same level of preservative function as the synthetic preservative.

Further, according to the results of the measurement of the preservative effect, the natural preservative composition of the present invention synergizes each other only when the extract of Rootaceae, Citrus and Pine .

By adding a certain amount of the natural preservative composition as described above to the wet tissue, or by adding a certain amount of the pharmaceutical composition of the natural preservative composition to the wet tissue, the wet tissue provides an excellent preservative function free from adverse effects on the human body.

Experimental Example 2: Skin stimulation experiment

I. Test Method: Repeat Insult Patch Test

1. Subjects: 10 healthy adults (age 20 ~ 35, male 5) who meet the criteria for selection and who do not qualify for exclusion and who have agreed to take the test And 5 women).

2. Application site: Forearm (Forearm)

3. Test substance: Comparative Example 1 (preservative treatment) / Comparative Example 1 (no preservative treatment) / Comparative Example 3 (no treatment) / Example 2

4. Test method

(1) Induction Phase: 9 times / 3 weeks (24 hour closure pills are performed every other day,

(2) Resting Period: 2 weeks

(3) Challenge Phase: 48 hours Closed After application, removed 1 hour, 24 hours, 48 hours

(4) Confirmation test: Only for subjects suspected of positive reaction.

5. Acceptance criteria

(1) Stimulation and Cumulative Stimulation: Visual evaluation was performed 1 hour after the removal of each patch, and scores were given according to the criteria of [Table 1]. The obtained results were evaluated according to the mean irritation index [Table 2]. The average irritation index was obtained from the following equation.

Symbols to record patch test results for induction phase Grade Characteristics 0 No reaction One Mild erythema 2 Intense erythema 3 Intense erythema with edema 4 Intense erythema and / or edema leading to a
bullae detachment with or without erosion

Average stimulus index and acceptance criteria Mean Irritation Index (MII) Criteria Lower than 0.25 Non irritant 0.25 to 1 not included Slightly irritant 1 to 2 not included Mean irritant 2 to 3 not included Very irritant 3 to 4 Very severely irritant

* Mean irritation index (MII) = total reading 3 times / (number of subjects X 3)

(2) Skin sensitization potential: The skin sensitization potential was measured at the origin of the lesion that was applied during induction and at the naive site of the new lesion. Based on the degree of skin reaction, Respectively.

Symbols to record patch test for sensitization Grade Characteristics 1 (+?) Doubtful reaction; faint erythema only 2 (+) Weak positive reaction; erythema, infiltration, possibly papules 3 (++) Strong positive reaction; erythema, infiltration, papules, vesicles 4 (+++) Extreme positive reaction; intense erythema and infiltration and coalescing vesicles

The following cases are considered to be allergenic potentials.

- Depending on the subject, the response phase is more prominent in the challenge phase than in the induction phase.

- If the intensity of the reaction is 3 or more, or if the reaction is allergy-specific (diffuse, papule, edema)

- Considering the characteristics of the cosmetics, it is checked based on the persistence rather than the intensity of the reaction.

The following cases are Hypoallergenic.

- There is no person with a clear positive reaction among all the subjects

division Comparative Example 1
(Preservative treatment) Comparative Example 1
(Without preservatives) Comparative Example 3
(Untreated) Example 2 Average skin irritation 25.0 3.0 1.0 2.0 Irritability judgment 20.0 2.0 1.0 1.0

As shown in the above Table 5, the composition containing the natural preservative (Example 2) according to the present invention and the antiseptic (Comparative Example 1) composition having little skin irritation had similar results in terms of skin safety, (Comparative Example 1), it can be seen that the stimulus is reduced considerably for the composition that is preserved (Comparative Example 1).

Claims (5)

10% to 40% by weight of roots extract; 10% to 30% by weight citrus extract; And 15 to 40% by weight of a pine needle extract. [3] The composition of claim 1, wherein the Root Root Extract is formed by pulverizing roots of Root Root to form Root Root, and pressing Root Root Root to obtain a Root Root Root Root Extract. [3] The composition of claim 1, wherein the citrus extract is formed by crushing the skin of citrus fruits to form a citrus crush, and compressing the citrus crush to obtain a liquid citrus extract. [3] The composition of claim 1, wherein the pine needle extract is obtained by pulverizing pine leaves to form pine needle pulverized material, and pressing the pine needle pulverized material to obtain a liquid pine needle extract. The method of claim 1, wherein the wetting agent composition