KR20210007577A - Antimicrobial composition comprising Morus alba bark extract, Brussels sprout extract, Soapberry extract and Angelica keiskei extract - Google Patents

Abstract

The present invention relates to an antibacterial composition comprising: a Morus alba bark extract; a Brassica oleracea var. gemmifera extract; a Sapindus mukorossi extract; and an Angelica keiskei extract and, more specifically, to an antibacterial composition derived from a natural product which can replace chemical antibacterial substances causing a lot of skin irritation. The antibacterial composition according to the present invention can be extracted from the natural product to reduce skin irritation, and can be used more efficiently by exerting an antibacterial effect against various microorganisms.

Description

Antimicrobial composition comprising Morus alba bark extract, Brussels sprout extract, Soapberry extract and Angelica keiskei extract}

The present invention relates to a natural antimicrobial composition, and relates to an antimicrobial composition for skin hypoallergenic, including a cornea extract, a bell cabbage extract, a soft nut extract, and a sinensis extract.

Preservatives are added to prevent spoilage of substances, and are added to foods and cosmetics. Since most cosmetics contain water, it is difficult to maintain quality, such as contamination by microorganisms such as bacteria or fungi or deformation of cosmetic formulations. In the case of food, various preservation methods such as pickling or drying with salt or vinegar are used, but due to the nature of cosmetics, it is difficult to remove moisture or add salt or vinegar. Therefore, cosmetics essentially contain preservatives, and various chemicals are added to various cosmetics that we use every day.

For example, parabens have been added to most cosmetics because their production cost is relatively inexpensive, they are easy to apply to cosmetics because of their odorless, colorless, and tasteless properties, and can effectively counter a wide range of contaminants. However, after the harmfulness of the paraben component was announced from several studies, a paraben-free product was developed, and it has been replaced by phenoxyethanol, 1,2-alkanediol-based compounds, and the like.

Phenoxyethanol is often used instead of parabens as a synthetic preservative, but it has a side effect of exacerbating dermatitis or eczema by irritating the skin mucosa, so its use is limited to less than 1%. 1,2-alkanediol compounds are also used as paraben replacements, but concerns about skin irritation still remain due to unproven ingredients.

Republic of Korea Patent Registration No. 10-1533195 (registered on June 25, 2015) relates to a safe wet tissue composition using natural preservatives, and a mixture containing Deodeok extract, citrus extract, and pine needle extract is described as a natural preservative composition. Republic of Korea Patent Registration No. 10-1525976 (registered on May 29, 2015) discloses a natural complex preservative composition containing a yongacho extract, a yellow lotus extract and a yongjeongcha extract.

An object of the present invention is to provide an antimicrobial composition derived from natural products that can replace chemical antimicrobial substances with high skin irritation.

The present invention provides an antimicrobial composition comprising an extract of Sangbaekpi, a drop cabbage extract, a soft nut extract, and an extract of Sinseoncho.

In the present invention, the extract of the serrata chinensis, cabbage extract, and fresh vinegar extract is preferably an ethanol extract, more preferably an ethyl acetate fraction of the ethanol extract.

In the present invention, the softnut extract is preferably a water extract.

In addition, it provides a cosmetic composition comprising 0.0001 ~ 3% (w / w) of the antibacterial composition comprising the extract, bell cabbage extract, soft nut extract and Sinseoncho extract of the present invention.

The present invention provides an antimicrobial composition derived from natural products that can replace chemical antimicrobial substances having high skin irritation.

The antimicrobial composition according to the present invention can be extracted from natural products to reduce skin irritation, and can be used more efficiently by exerting antibacterial effects against various microorganisms.

In addition, the present invention can expect skin whitening effect and skin moisturizing effect exerted from each natural product by applying the antibacterial composition including the extract of the cornea extract, the cabbage extract, the soft nut extract and the extract of Sinseoncho in the cosmetic composition.

1 is a result of an antimicrobial activity test against Staphylococcus aureus KCTC 3881 of Preparation Examples 1 to 3 of the present invention.
2 is a result of an antibacterial activity test against Pseudomonas aeruginosa KCTC 1750 of Preparation Examples 1 to 3 of the present invention.
3 is a result of an antibacterial activity test for Escherichia coli of Preparation Examples 1 to 3 of the present invention.
4 is a result of an antibacterial activity test for Enterobacter cloacae of Preparation Examples 1 to 3 of the present invention.
5 is an antibacterial activity test result for Candida albicans of Preparation Examples 1 to 3 of the present invention.
Figure 6 is a test result of antibacterial activity against the assay mold ( Aspergillus niger ) of Preparation Examples 1 to 3 of the present invention.
7 is a result of an antimicrobial activity test against Staphylococcus aureus KCTC 3881 of a composition in which Preparation Example 1 and ethylhexyl glycerin of the present invention are mixed.
8 is a result of an antibacterial activity test against Pseudomonas aeruginosa KCTC 1750 of a composition in which Preparation Example 1 and ethylhexyl glycerin of the present invention are mixed.
9 is a result of an antimicrobial activity test against Escherichia coli of a composition in which Preparation Example 1 of the present invention and ethylhexyl glycerin are mixed.
10 is a result of an antibacterial activity test against Enterobacter cloacae of a composition in which Preparation Example 1 of the present invention and ethylhexyl glycerin are mixed.
11 is a result of an antimicrobial activity test for Candida albicans of a composition in which Preparation Example 1 of the present invention and ethylhexyl glycerin are mixed.
12 is a result of an antimicrobial activity test against a test mold ( Aspergillus niger ) of a composition in which Preparation Example 1 of the present invention and ethylhexyl glycerin are mixed.

Preservatives added to cosmetics are generally chemical substances, and depending on their content, they may cause skin irritation, inflammation and allergies. Accordingly, in the present invention, an antimicrobial composition using natural plant extracts applicable to cosmetics is developed and provided.

Accordingly, the present invention provides an antimicrobial composition comprising a Sangbaekpi extract, a bell cabbage extract, a soft nut extract, and a Sinseoncho extract.

Sangbaekpi is a dried root bark of a mulberry tree ( Morus alba L.) or a plant of the same genus, and is mainly used as a medicinal material to remove inflammation of the lungs and stop coughing.

Bell cabbage is also called bell pepper ( Brassica oleracea var. gemmifera), and unlike ordinary cabbage, the stem grows long and leaves are rarely attached, and lamb cabbage hangs in a bell shape from the axilla. It contains large amounts of protein, vitamin A, and vitamin C.

Softnut is the fruit of Sapindus mukorossi . The no-patient tree is distributed in Korea, Japan, Taiwan, and China, and can be found mainly in Jeju Island, Jeolla-do and Gyeongsang-do. Fruit seeds are sometimes used as medicine.

Angelica keiskei is a perennial plant of the dicotyledonous umbel, Ranunculus. Young leaves are juiced or eaten as herbs, and contain large amounts of vitamin B1, vitamin B2, vitamin C, and calcium.

In the present invention, the extract may be prepared according to a conventional method known in the art, that is, using a conventional solvent under the conditions of a conventional temperature and pressure, for example, water, anhydrous of 1-4 carbon atoms, or Solvent extraction method using an extraction solvent selected from the group consisting of water-containing lower alcohol, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane and mixtures thereof, supercritical It can be obtained through any one extraction method selected from the extraction method and the ultrasonic extraction method.

In the present invention, for the extract of the cornea extract, the cabbage extract, and the sinensis extract, an ethanol extract may be preferably used, and more preferably an ethyl acetate fraction of the ethanol extract may be used. In the present invention, the softnut extract is preferably a water extract.

In addition, the present invention provides a cosmetic composition comprising 0.0001 to 3% (w / w) of the antibacterial composition comprising the extract, cabbage extract, soft nut extract, and Sinseoncho extract.

In order to evaluate the room buoyancy of cosmetic and receive the final fit is determined as a representative Staphylococcus aureus (Staphylococcus aureus), a Pseudomonas aeruginosa (Pseudomonas aeruginosa), E. coli (Escherichia coli), Enterobacter claw ACCA (Enterobacter cloacae), Candida albicans (Candida albicans ) and Aspergillus niger 6 species should show antibacterial effects.

As a result of testing the antimicrobial effect of each of the extract of the present invention, the extract, the cabbage extract, the soft nut extract, and the extract of Sinseoncho, the extract showed the antibacterial effect against Staphylococcus aureus (gram-positive bacteria), and the cabbage extract was Candida albicans The antibacterial effect was shown against (yeast bacterium) and black mold, and the soft nut extract showed antibacterial effect against Candida albicans (yeast bacterium), and the Sinseoncho extract against black mold (Table 1).

In addition, the present invention conducted an experiment to confirm that the antibacterial effect is further enhanced when each natural extract having an antibacterial effect is mixed with an appropriate amount of conventional antibacterial substances. As a result of testing using phenoxyethanol as a chemical preservative and 1,2-hexanediol and ethylhexyl glycerin as chemical antibacterial substances, each extract was mixed with phenoxyethanol, 1,2-hexanediol, or ethylhexyl glycerin. When used in an appropriate amount, it was confirmed that not only the amount of chemical antibacterial substances added could be reduced, but also the antibacterial and antiseptic effects were enhanced by showing a synergistic effect when mixed with the chemical antibacterial substances.

In addition, in the present invention, the Sangbaekpi extract, the bell cabbage extract, the softnut extract, and the Sinseoncho extract are preferably 0.2-1 parts by weight of the Sangbaekpi extract, 0.5-2 parts by weight of the Bell cabbage extract, 0.1-2 parts by weight of the softnut extract, the Sinseoncho It is preferable to mix 0.5 to 2 parts by weight of the extract, more preferably 0.4 to 0.8 parts by weight of Sangbaekpi extract, 1 to 2 parts by weight of bell cabbage extract, 0.5 to 1 parts by weight of softnut extract, and 1 to 2 parts by weight of Sinseoncho extract. It is good.

Hereinafter, the content of the present invention will be described in more detail through the following examples and experimental examples. However, the scope of the present invention is not limited to the following examples and experimental examples, and includes modifications of equivalent technical ideas.

[Example 1: Preparation of extracts of Sangbaekpi, Bell cabbage, Softnut, Sinseoncho]

(1) Preparation of Sangbaekpi extract

The pulverized Sangbaek skin and 70% (v/v) ethanol were mixed at a weight ratio of 1:20, and extracted under reflux at 80° C. for 2 hours, followed by filtration to obtain an ethanol extract of Sangbaekpi. The ethanol extract of Sangbaekpi was concentrated at 50°C to obtain a concentrated powder of ethanol extract of Sangbaekpi.

After diluting the concentrated powder of the ethanol extract of the serrata in distilled water to a concentration of 10% (w/v), the diluted product and the same amount of ethyl acetate were mixed, and the ethyl acetate fraction layer was concentrated at 50°C to prepare a concentrated powder of the ethyl acetate fraction of the serrata. I did.

The concentrated powder of the upper bark ethyl acetate fraction was dissolved in a solvent (a solution of 1,3-butylene glycol and 70% (v/v) ethanol in a volume ratio of 3:7) at a concentration of 50% (w/v). At this time, to increase the solubility, the concentrated powder was first mixed with ethanol and then mixed with 1,3-butylene glycol.

(2) Preparation of drop cabbage extract

Drop cabbage extract was prepared in the same manner as the Sangbaekpi extract.

(3) Preparation of softnut extract

The pulverized softnut and distilled water were mixed at a weight ratio of 1:20, extracted with hot water at 80° C. for 2 hours, and filtered to obtain a hot water extract of the soft nut. The soft nut hot water extract was concentrated at 50° C. to obtain a concentrated powder of the soft nut hot water extract.

The concentrated powder of the Soapnut hot water extract was dissolved in a solvent (a solution obtained by mixing 1,3-butylene glycol and 70% (v/v) ethanol in a 3:7 volume ratio) at a concentration of 20% (w/v). At this time, to increase the solubility, the concentrated powder was first mixed with ethanol and then mixed with 1,3-butylene glycol. Then, it was filtered after centrifuging for 5 minutes at 6000 rpm.

(4) Preparation of Sinseoncho extract

Sinseoncho extract was prepared in the same manner as the Sangbaekpi extract.

[Experimental Example 1: Confirmation of the antibacterial effect of extracts of Sangbaekpi, Bell cabbage, Softnut, and Sinseoncho]

In this experiment, Staphylococcus aureus KCTC 3881), Pseudomonas aeruginosa KCTC 1750, Escherichia coli , Enterobacter cloacae , Candida albi of each natural extract prepared in Example 1 To confirm the antibacterial effect against Candida albicans and Aspergillus niger .

The antibacterial activity of the natural extract was measured by a disc diffusion method. The strain was inoculated into a cell line (saline) to be about 1×10 ⁶ ~10 ⁷ cfu/ml on a plate medium, and then uniformly spread with a sterile cotton swab. Place the sterilized paper disk on a solid plate and absorb the sample by concentration so that it becomes 0.05 ml/disc or 0.026 ml/disc. Bacteria are incubated at 37°C for 24 hours, and fungi are incubated at 25°C for 72 hours. The antibacterial zone (clea zone) was confirmed.

The experimental results are shown in Table 1 (unit: mm) below. Methylparaben was used as a control.

Sangbaek blood Bell cabbage Softnut Sinseoncho Methylparaben yellow
Staphylococcus 3.7±0.6
(2.5-10%) - - - 4.7±1.2
(5%) Pseudomonas aeruginosa - - - - - Coli - - - - - Enterobacter
Cloaca - - - - - Candida
Albicans - 0.5-1
(5-10%) 1-3
(2-10%) - - Black mold - 1-1.5
(2-10%) - 3-5
(2-3%) -

As shown in Table 1 above, the antibacterial zone against Staphylococcus aureus (Gram-positive bacteria) is 3.7 mm, and the drop cabbage has an antibacterial zone of 0.5 to 1 mm against Candida albicans (yeast bacteria), and the antibacterial zone against black mold. This 1~1.5 mm, the softnut extract had an antibacterial zone of 1~3 mm against Candida albicans (yeast bacteria), and the Sinseoncho extract had an antibacterial zone of 3~5 mm against black mold. In particular, the antimicrobial zone for Staphylococcus aureus was 4.7 mm in methylparaben at a concentration of 5% (v/v), whereas the antibacterial zone of 3.7 mm appeared in the extract of S. It has been identified as a substitute for.

[Experimental Example 2: Confirmation of the antiseptic efficacy of extracts of Sangbaekpi, Bell cabbage, Softnut, Sinseoncho]

In this experiment, the extracts prepared in Example 1 above were applied to the cosmetic composition to confirm the antiseptic effect by applying the extracts of Sangbaekpi, Bell Cabbage, Softnut, and Sinseoncho. Seven strains used in the evaluation of preservative efficacy are shown in Table 2 below.

Bacteria group Gram positive bacteria (G+) Staphylococcus aureus KCTC 3881 Gram negative bacteria (G-) Pseudomonas aeruginosa KCTC 1750 Enterobacter cloacae KCTC 2361 Escherichia coli KCTC 2571 Yeast & Mold group Yeast (Y) Candida albicans KCTC 7965 Black mold (M) Aspergillus niger KCTC 6985

In the evaluation of preservative efficacy, each extract prepared in Example 1 was mixed with 50 g of a cosmetic composition to which no preservative was added, and strains were inoculated for each group, and the trend of microbial reduction was observed for 15 days.

(1) Evaluation of antiseptic efficacy of Sangbaekpi extract

In emulsion formulation cosmetics, Staphylococcus aureus KCTC 3881 as Gram-positive bacteria (Bac G(+)), Pseudomonas aeruginosa KCTC 1750 as Gram-negative bacteria (Bac G(-)), Candida albi as yeast Aspergillus niger was inoculated with Candida albicans and mold to observe the microbial decline.

The initial number of bacteria was shown in Table 3 below (unit: cfu/mL (g)).

Bac G(+) Bac G(-) Yeast(Y) Mold(M) 2.6×10 ⁵ 1.5×10 ⁶ 1.6×10 ⁵ 2.14×10 ⁵

As a result of inoculating the bacteria into 50 g of the antiseptic emulsion, it was confirmed that the microorganisms steadily proliferated for 15 days (Table 4, the number of bacteria unit: cfu/mL (g)).

Elapsed day (days) One 3 5 8 10 12 15 Dilution factor
(Bac/Y·M) 10 ^-3 /10 ^-2 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 Bac G+ 5.1×10 ⁴ 4.0×10 ⁴ 2.9×10 ⁴ 1.0×10 ⁴ 1.1×10 ⁴ 1.2×10 ⁴ 1.1×10 ⁴ G- 1.36×10 ⁵ 3.0×10 ³ 3.2×10 ⁵ 9.07×10 ⁴ 5.5×10 ⁴ 1.4×10 ⁴ 1.3×10 ⁴ Y 1.5×10 ³ 1.8×10 ⁴ 1.56×10 ⁴ 2.03×10 ⁴ 2.0×10 ⁴ 2.1×10 ⁴ 2.2×10 ⁴ M 2.0×10 ² 2.1×10 ² 1.9×10 ² 2.0×10 ² 1.2×10 ² 1.0×10 ² 1.5×10 ²

Phenoxyethanol 0.3% (w/w) and ethylhexyl glycerin 0.3% (w/w) were added to 50 g of the preservative-free emulsion, and as a result of inoculating the bacteria, gram-positive bacteria were killed within 4 days, and gram-negative bacteria, yeast bacteria, The fungus was killed within 8 days, and it was confirmed that the cosmetic preservative effect is suitable (Table 5, number of bacteria unit: cfu/mL (g)).

Elapsed day (days) One 3 4 8 10 12 15 Dilution factor
(Bac/Y·M) 10 ^-3 /10 ^-2 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 Bac G+ 4.0×10 ³ 2.0×10 ¹ - - - - - G- 5.0×10 ³ 1.4×10 ² 1.1×10 ² - - - - Y 1.2×10 ⁴ 2.1×10 ² 1.8×10 ² - - - - M 8.1×10 ³ 1.8×10 ² 1.3×10 ² - - - -

To 50 g of the preservative-free emulsion, 0.8% (w/w), ethylhexyl glycerin 0.2% (w/w), and 1,2-hexanediol 0.2% (w/w), prepared in Example 1, were added. As a result of inoculating the bacteria, the number of gram-positive bacteria, gram-negative bacteria, and yeast bacteria decreased sharply after 1 day, and the mold bacteria had excellent antiseptic effects from the first day, and all were killed within 5 days, confirming that the cosmetic preservative effect was suitable (Table 6 , The number of bacteria unit: cfu/mL(g)).

Elapsed day (days) One 3 5 8 10 12 15 Dilution factor
(Bac/Y·M) 10 ^-3 /10 ^-2 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 Bac G+ 7.0×10 ³ 2.0×10 ¹ - - - - - G- 7.0×10 ³ 3.0×10 ¹ - - - - - Y 1.1×10 ³ 2.0×10 ¹ - - - - - M 1.0×10 ¹ 1.0×10 ¹ - - - - -

As shown in Tables 5 and 6, the addition ratio of ethylhexylglycerin and 1,2-hexanediol can be reduced by the addition of the extract of serrata, thereby reducing skin irritation due to the use of ethylhexylglycerin and 1,2-hexanediol. It was determined that it could be reduced.

(2) Evaluation of antiseptic efficacy of bell cabbage extract

Since the drop cabbage extract does not have an antiseptic effect on bacteria, the experiment was conducted by inoculating yeast and fungi. The emulsion formulation was inoculated with Candida albicans as yeast and Aspergillus niger as mold to observe the microbial reduction trend.

The initial number of bacteria was shown in Table 7 below (unit: cfu/mL (g)).

Bac G(+) Bac G(-) Yeast(Y) Mold(M) - - 9.0×10 ⁵ 8.5×10 ⁵

As a result of inoculating the bacteria into 50 g of the antiseptic emulsion, it was confirmed that the microorganisms steadily proliferated for 18 days (Table 8, the number of bacteria unit: cfu/mL (g)).

Elapsed day (days) One 4 8 11 18 Dilution factor
(Bac/Y·M) 10 ^-3 /10 ^-2 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 Bac G+ - - - - - G- - - - - - Y 2.2×10 ⁴ 1.4×10 ⁴ 2.6×10 ⁴ 2.8×10 ⁴ 2.8×10 ⁴ M 7.1×10 ³ 3.6×10 ³ 6.0×10 ³ 5.4×10 ³ 1.6×10 ³

To 50 g of the preservative-free emulsion, 2% (w/w) of the bell cabbage extract prepared in Example 1, 0.2% (w/w) of ethylhexyl glycerin, and 0.4% (w/w) of 1,2-hexanediol were added. As a result of inoculating the bacteria, the yeast bacteria were killed within 4 days, and the mold bacteria were killed within 6 days, confirming that the cosmetic preservative efficacy was suitable (Table 9, number of bacteria unit: cfu/mL (g)).

Elapsed day (days) One 4 6 8 11 13 15 Dilution factor
(Bac/Y·M) 10 ^-3 /10 ^-2 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 Bac G+ - - - - - - - G- - - - - - - - Y 2.7×10 ⁴ - - - - - - M 2.2×10 ⁴ 5.0×10 ¹ - - - - -

To 50 g of an antiseptic emulsion, 4% (w/w) of the bell cabbage extract prepared in Example 1, 0.1% (w/w) of ethylhexyl glycerin, and 0.3% (w/w) of 1,2-hexanediol were added. As a result of inoculating the bacteria, both yeast and fungi were killed within 4 days, and it was confirmed that the cosmetic preservative effect is suitable (Table 10, number of bacteria unit: cfu/mL (g)).

Elapsed day (days) One 4 8 11 18 Dilution factor
(Bac/Y·M) 10 ^-3 /10 ^-2 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 Bac G+ - - - - - G- - - - - - Y 1.5×10 ⁴ - - - - M 7.9×10 ³ - - - -

As shown in Tables 9 and 10, it was confirmed that the antibacterial effect was excellent even though the ratio of the drop cabbage extract was increased and the ratio of ethylhexyl glycerin and 1,2-hexanediol was reduced. Through this, it was determined that skin irritation caused by the use of ethylhexyl glycerin and 1,2-hexanediol could be reduced.

(3) Evaluation of antiseptic efficacy of softnut extract

In emulsion formulation cosmetics, Staphylococcus aureus KCTC 3881 as Gram-positive bacteria (Bac G(+)), Pseudomonas aeruginosa KCTC 1750 as Gram-negative bacteria (Bac G(-)), Candida albi as yeast Aspergillus niger was inoculated with Candida albicans and mold to observe the microbial decline.

The initial number of bacteria was as shown in Table 11 below (unit: cfu/mL(g)).

Bac G(+) Bac G(-) Yeast(Y) Mold(M) 7×10 ⁶ 1.1×10 ⁷ 2.5×10 ⁶ 7×10 ⁵

As a result of inoculating the bacteria into 50 g of the antiseptic emulsion, it was confirmed that the microorganisms steadily proliferated for 15 days (Table 12, the number of bacteria unit: cfu/mL (g)).

Elapsed day (days) One 4 6 8 11 13 15 Dilution factor
(Bac/Y·M) 10 ^-3 /10 ^-2 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 Bac G+ 6.0×10 ³ 7×10 ⁵ TNTC TNTC TNTC TNTC TNTC G- 4.2×10 ⁵ TNTC TNTC TNTC TNTC TNTC TNTC Y 8.6×10 ⁴ TNTC TNTC TNTC TNTC TNTC TNTC M 2.6×10 ⁴ 1.5×10 ³ 2×10 ³ 2.8×10 ² 3×10 ¹ 1×10 ¹ 1×10 ¹

Note) TNTC: too numerous to count

Ethylhexyl glycerin 0.2% (w/w) and 1,2-hexanediol 0.4% (w/w) were added to 50 g of the preservative-free emulsion, and as a result of inoculating the bacteria, Gram-positive bacteria, Gram-negative bacteria and yeast bacteria were found within 4 days. Although killed, the fungal bacteria were also present on the 11th day (Table 13, bacterial count unit: cfu/mL(g)).

Elapsed day (days) One 4 6 8 11 13 15 Dilution factor
(Bac/Y·M) 10 ^-3 /10 ^-2 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 Bac G+ 6.6×10 ⁴ - - - - - - G- 2.9×10 ⁵ - - - - - - Y 2.8×10 ⁴ - - - - - - M 1.8×10 ⁴ 2.8×10 ³ 2.3 10 ³ 2.2×10 ³ 1.6×10 ³ - -

To 50 g of an antiseptic emulsion, 1% (w/w) of the softnut extract prepared in Example 1, 0.2% (w/w) of ethylhexyl glycerin, and 0.4% (w/w) of 1,2-hexanediol were added. As a result of inoculation of the bacteria, Gram-positive bacteria and yeast bacteria died within 4 days, and Gram-negative bacteria rapidly decreased on the 4th day and died within 6 days, and the mold bacteria rapidly decreased after 4 days and died within 11 days, so cosmetic preservative efficacy is suitable. (Table 14, bacterial count unit: cfu/mL (g)).

Elapsed day (days) One 4 6 8 11 13 15 Dilution factor
(Bac/Y·M) 10 ^-3 /10 ^-2 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 Bac G+ 1.2×10 ⁵ - - - - - - G- 1.6×10 ⁵ 2.0×10 ¹ - - - - - Y 4.1×10 ⁴ - - - - - - M 2.1×10 ⁴ 1.5×10 ² 2.0×10 ¹ 2.0×10 ¹ - - -

As shown in Tables 13 and 14, it was determined that the softnut extract exhibited particularly antiseptic effects against mold bacteria.

(4) Evaluation of antiseptic efficacy of Sinseoncho extract

Since the extract of Sinseoncho chinensis does not have an antiseptic effect on bacteria, the experiment was conducted by inoculating yeast and fungi. The emulsion formulation was inoculated with Candida albicans as yeast and Aspergillus niger as mold to observe the microbial reduction trend.

The initial number of bacteria was as shown in Table 15 below (the number of bacteria unit: cfu/mL (g)).

Bac G(+) Bac G(-) Yeast(Y) Mold(M) - ^- 6.0×10 ⁵ 3.0×10 ⁵

As a result of inoculating the bacteria into 50 g of the antiseptic emulsion, it was confirmed that the fungal bacteria steadily proliferated for 15 days (Table 16, number of bacteria unit: cfu/mL(g)).

Elapsed day (days) One 4 5 12 13 15 Dilution factor
(Bac/Y·M) 10 ^-3 /10 ^-2 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 Bac G+ - - - - - - G- - - - - - - Y 2.4×10 ⁴ - - - - - M 1.1×10 ⁴ 6.4×10 ² 4.4×10 ² 1.3×10 ² 2.2×10 ² 1.8×10 ²

Ethylhexyl glycerin 0.2% (w/w) and 1,2-hexanediol 0.4% (w/w) were added to 50 g of the preservative-free emulsion, and as a result of inoculating the bacteria, mold bacteria were also present on the 11th day (Table 17 , The number of bacteria unit: cfu/mL(g)).

Elapsed day (days) One 4 8 11 18 Dilution factor
(Bac/Y·M) 10 ^-3 /10 ^-2 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 Bac G+ - - - - - G- - - - - - Y 2.6×10 ⁴ - - - - M 2.2×10 ⁴ 1.7×10 ³ 2.6×10 ³ 3.0×10 ¹ -

To 50 g of the preservative-free emulsion, 2% (w/w) of the fresh vinegar extract prepared in Example 1, 0.2% of ethylhexyl glycerin (w/w), and 0.4% of 1,2-hexanediol (w/w) were added, and As a result of inoculating the bacteria, the number of yeast bacteria on the first day was relatively decreased, and the mold bacteria were also killed within 8 days, confirming that the cosmetic preservative effect was suitable (Table 18, bacteria number unit: cfu/mL (g)).

Elapsed day (days) One 4 8 11 18 Dilution factor
(Bac/Y·M) 10 ^-3 /10 ^-2 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 10 ^-1 /10 ^-1 Bac G+ - - - - - G- - - - - - Y 1.7×10 ⁴ - - - - M 1.9×10 ⁴ 6.5×10 ² - - -

As shown in Tables 17 and 18, it was judged that the extract of Sinseoncho chinensis exhibited particularly antiseptic effects against fungi.

[Example 2: Preparation of mixed composition]

In Experimental Example 1, the antibacterial effect of the extracts of Sangbaekpi, Bell cabbage, Softnut, and Sinseoncho was confirmed. Accordingly, in this example, in order to prepare a new antimicrobial composition showing a synergistic effect by mixing each of the natural extracts prepared in Example 1, each of the natural extracts was mixed according to Table 19 (unit: mixed weight ratio).

Natural extracts Manufacturing Example 1 Manufacturing Example 2 Manufacturing Example 3 Sangbaekpi extract 0.8 0.4 0.2 Bell Cabbage Extract 2 One 2 Softnut extract One 0.5 2 Sinseoncho extract 2 One 2

[Experimental Example 3: Confirmation of Antibacterial Effect of Mixed Composition]

In this experimental example, Staphylococcus aureus KCTC 3881, Pseudomonas aeruginosa KCTC 1750), Escherichia coli , Enterobacter cloaca of the mixed antiseptic compositions of Preparation Examples 1 to 3 prepared in Example 2 Enterobacter cloacae ), Candida albicans ( Candida albicans ), and black fungi ( Aspergillus niger ) to confirm the antibacterial effect.

The antimicrobial activity of the mixed composition was measured by a disc diffusion method. The strain was inoculated into a cell line (saline) to be about 1×10 ⁶ ~10 ⁷ cfu/ml on a plate medium, and then uniformly spread with a sterile cotton swab. Place the sterilized paper disk on a solid plate and absorb the sample by concentration so that it becomes 0.05 ml/disc or 0.026 ml/disc. Bacteria are incubated at 37°C for 24 hours, and fungi are incubated at 25°C for 72 hours. The antibacterial zone (clea zone) was confirmed.

The experimental results are shown in Table 20 (unit: mm) and FIGS. 1 to 6 below.

Strain Manufacturing Example 1 Manufacturing Example 2 Manufacturing Example 3 Methylparaben Staphylococcus aureus 9.8±3.1 3.8±1.5 5.8±2.8 4.7 Pseudomonas aeruginosa 3 - One - Coli 4 One One - Enterobacter cloaca 5 - 3±1.4 - Candida albicans 5 - - - Black mold 5 - 5 -

As a result of comparison with the antibacterial effect (Table 1) of the single extracts of the extracts of Sangbaek skin, bell cabbage, soft nut, and fresh vinegar identified in Experimental Example 1, it was found that the antibacterial effect was further enhanced when the mixture prepared in Example 2 was treated. . In the case of Staphylococcus aureus, the antibacterial zone was expanded in Preparation Example 3 (5.8 mm) and Preparation Example 1 (9.8 mm) when compared to the treatment with a single extract of the cornea (3.7 mm), and in the case of Candida albicans, a single drop of cabbage extract was treated. The antibacterial zone was expanded to 5 mm in Preparation Example 1 compared to the time (0.5 to 1 mm) or the treatment with a single softnut extract (1 to 3 mm), and in the case of black mold, when the single drop of cabbage was treated (1 to 1.5 mm) In comparison, it was confirmed that the antibacterial zone was expanded to 5 mm in Preparation Example 1.

In addition, it was confirmed that the antibacterial effect was exhibited even for the strain that did not show the antibacterial effect when treated with a single substance. In the single treatment of the extract of S. aureus, an antibacterial zone was not found in all strains except Staphylococcus aureus, but in Preparation Example 2, which is a mixed composition, an antibacterial zone was found in E. It was found and confirmed that the antibacterial zone was expanded, and in Preparation Example 1, the antibacterial zone was discovered and the antibacterial zone was expanded for all strains.

[Experimental Example 4: Confirmation of the antiseptic efficacy of the mixed composition]

In this Experimental Example, the antiseptic efficacy of Preparation Example 1, in which the antibacterial activity was confirmed in all six species through Experimental Example 3, was examined.

Preparation Example 1 confirmed the preservative efficacy by mixing 0.2% (w/w) ethylhexyl glycerin with respect to the weight of the diluted product diluted to 0.5% (v/v) or 1% (v/v) in distilled water. I did.

The experimental results are shown in Table 21 (unit: mm) and FIGS. 7 to 12 below.

Strain Manufacturing Example 1 Preparation Example 1 1% dilution
(One%) Preparation Example 1 0.5% diluted product and ethylhexyl glycerin mixture
(0.5%+E0.2%) Preparation Example 1 Mixing 1% diluted product and ethylhexyl glycerin
(1%+E0.2%) Staphylococcus aureus 9.8±3.1 2 2 2 Pseudomonas aeruginosa 3 One 0.5 3 Coli 4 0.5 0.5 0.5 Enterobacter cloaca 5 3 2 5 Candida albicans 5 One 5 2 Black mold 5 One 2 One

As shown in Table 21 and FIGS. 7 to 12, the size of the antibacterial zone was similar when the 1% dilution of Preparation Example 1 was treated and the 0.5% dilution of Preparation Example 1 was mixed with ethylhexyl glycerin, and in particular Candida In the case of albicans, it was confirmed that the antibacterial zone was effectively expanded when ethylhexyl glycerin was added.

In addition, it was confirmed that the antibacterial zone of Pseudomonas aeruginosa and Enterobacter cloaca effectively expanded when 1% diluted product of Preparation Example 1 and ethylhexyl glycerin were mixed.

Claims (5)

An antimicrobial composition comprising a cornea extract, a bell cabbage extract, a soft nut extract and a sinensis extract.
The method of claim 1,
The Sangbaekpi extract, bell cabbage extract, Sinseoncho extract,
Antimicrobial composition, characterized in that the ethanol extract.
The method of claim 1,
The Sangbaekpi extract, bell cabbage extract, Sinseoncho extract,
Antimicrobial composition, characterized in that the ethyl acetate fraction of the ethanol extract.
The method of claim 1,
The softnut extract,
Antimicrobial composition, characterized in that the water extract.
A cosmetic composition comprising 0.0001 to 3% (w/w) of the antimicrobial composition of claim 1.

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