KR102490236B1 - Preservative composition for cosmetics. - Google Patents

Abstract

Obtaining an extract by performing hot water extraction by mixing and heating the selected and washed high ginseng raw material and the selected and washed nut gall nut raw material at a ratio of 1: 1; Obtaining a saturated rock salt solution by putting the rock salt in a stirrer and dissolving it while stirring at low speed so that it is saturated in distilled water; Obtaining a complex mineral solution obtained as an aqueous solution by diluting the complex mineral concentration 100 times or more; A first composite solution preparation step of mixing the prepared solution and extract at a predetermined ratio and stirring for 24 hours or more; Gosam and gall nut extract consisting of an impregnation step (S150) of mixing 0.2 to 1.0% by weight of the composition of the first composite solution prepared from the prepared first composite solution with the cosmetic composition and stirring in a stirrer to complete the cosmetic raw material Cosmetics as an active ingredient have an antibacterial effect without the addition of chemical preservatives.

Description

A preservative composition for cosmetics containing extracts of ginseng and gall nut as active ingredients {Preservative composition for cosmetics.}

The present invention relates to a cosmetic preservative composition containing extracts of gosam and gall nut as active ingredients, and extracts of gosam and nut gall that have antibacterial effects without the addition of preservatives by mixing hot-water extracted gosam and nut gall extracts, a complex mineral solution, and a saturated rock salt solution. It relates to a cosmetic preservative composition containing as an active ingredient and a manufacturing method thereof.

In general, preservatives are substances added to prevent deterioration of products. They kill or prevent the growth of bacteria and fungi, and are added to foods, medicines, and cosmetics that must be protected from microbial proliferation or decay. In particular, chemical preservatives are used.

Recently, after more than 50 years of using chemical preservatives, various studies have revealed various problems and side effects of artificial preservatives, and the dangers are emerging. Recently, experts point out that children are more likely than any previous generation to face many problems, such as the development of all kinds of cancer and childbirth difficulties, in the future. Fertility experts, oncologists, and environmentalists believe that these chemicals are associated with skin irritation, cytotoxicity, various skin diseases, central nervous system paralysis, breast cancer, hormone disruption, and decreased sperm count.

On the other hand, as an essential ingredient used in cosmetics, a preservative component to prevent rancidity of the contents is required. Parabens, which have traditionally been used as preservatives for cosmetics or household products, have been frequently discussed as harmful substances for several years.

In addition, as each country enacts cosmetic laws focused on strengthening regulations on ingredients contained in cosmetics that may affect carcinogens or reproductive organs, awareness of preservatives for cosmetics is increasing, and the need for alternative cosmetic preservatives that are not harmful and toxic this is rising Therefore, it is necessary to develop a safer and more cosmetic preservative that can replace parabens.

In the natural world, there are effective substances that have the function of antibiotics, and in the Orient, these substances have been extracted and used through medicinal herbs. These natural antibiotic substances do not develop bacterial resistance and are known to act harmlessly to normal cells, among which there are gosam and nut gall.

Gosam ( Sophora flavescens Alton) uses the peeled root of Gosam in Korea, China, and Japan. The name Gosam comes from the Chinese character Go (苦) because it tastes bitter, and Sam (參) because its efficacy is similar to that of ginseng. It is cylindrical, and the outer surface is dark tan, with distinct vertical wrinkles and horizontally long skin. When the perianth is peeled off, it is yellowish white, and the folded side is slightly fibrous. It grows in the grass or roadside at the foot of a mountain. It is dug out in spring and autumn, washed clean, sliced, dried in the sun, and used as a medicine. Decoct 3-10g and take. When used externally, use an appropriate amount.

Gosam has a peculiar smell and is very bitter and cold. It is used for dysentery, phlegmon, genital pruritus, skin itching caused by lower moist fever, and is used when there is pain in not being able to urinate due to bladder openness. In oriental medicine, dried roots are called gosam, and they are prescribed for indigestion, neuralgia, hepatitis, jaundice, and hemorrhoids. It is good for bad breath caused by excessive heat in the stomach, dry mouth, and bleeding gums. It is also effective in treating symptoms such as enteritis, jaundice, food poisoning, and constipation. It is effective in the prevention and treatment of constipation, facilitates the excretion of urine, and is effective in heart disease as it stimulates the discharge of feces by stimulating the stool and bowel bleeding caused by weakness or disease. To treat tumors or pain. Dry stomach, anthelmintic effect. It not only strengthens the stomach, but also contains strong bactericidal ingredients, so it is also used as an insect repellent.

As for the pharmacological effects, leukopenia treatment and anti-radiation action, coronary blood flow increase, heart muscle strengthening, blood sugar lowering action, anti-tumor, antibacterial, immune suppression action, etc. have been reported. It contains alkaloids such as d-matrine, d-oxyma trine, d=sophoranol, and flavoids such as xanthohumol and isoxanthohumol.

Nut gall ( Rhus javanica L) is an insect hump made by parasitic fungus on the leaves of the red tree (nut gall tree) of the sumacaceae, and many of them resemble human ears in the shape of irregular pockets. It is hollow and very sour in taste. In oriental medicine, it has convergence, hemostasis, detoxification, and antibacterial effects, so it is prescribed for diarrhea, gastric ulcer, heart ulcer, cold sweat, oil well, bloody stool, hematuria, stomatitis, etc. It contains 50 to 60% of tannins, so it is a raw material for tannins, hair dyes, and inks.

Fire tree worm house (nut gall) contains 50-60% of tannin, which has strong astringent and soothing effects on diarrhea. When used with cucumber grass, the effect is enhanced. Its efficacy acts on the lungs, stomach and large intestine. It is used for coughs that do not heal for a long time due to deficient lungs, dysentery and diarrhea, prolapse and postpartum prolapse, thirst for drinking a lot of water, hematuria, nosebleeds, scrotal eczema, cracked hands and feet, hemostasis of wounds from tooth extraction, and treatment of inflammation.

Therefore, the present invention is a cosmetic preservative composition containing as active ingredients the natural herb extract and complex ion mineral with antibiotic effect, which can replace conventional chemical preservatives, and extracts of gosam and gall nut, which have natural preservative effects, and a method for producing the same. want to provide

Domestic Patent No. 10-1864263 discloses 1,2-hexanediol, caprylyl glycol, butylene glycol, pentylene glycol, ethylhexyl glycerin, rooibos extract, licorice extract, myrrh extract, perilla leaf extract , And a natural preservative composition for cosmetics having skin irritation relief and moisturizing power containing yucca extract, wherein the myrrh extract, perilla leaf extract, and yucca extract are each 0.1 to 0.3% by weight in the total composition, and the rooibos extract and licorice extract are Disclosed is a natural preservative composition for cosmetics having skin irritation relief and moisturizing power, characterized in that 0.1 to 0.5% by weight. Domestic Patent No. 10-2259004 is extracted from natural products, which can reduce skin irritation, and exhibit antibacterial effects against various microorganisms, which can be used more efficiently. Disclosed is a cosmetic preservative composition comprising Korean Patent Registration No. 10-1095789 discloses a non-aqueous cosmetic composition containing 0.1 to 50.0% by weight of caprylic/capric glyceride, 0.01 to 10.0% by weight of alpha-bisabolol, and 0.1 to 60.0% by weight of coconut oil. Characterized by, polyisobutene, ozokerite, ethylhexanoin, isostearyl maleate, candelilla wax, cosmetic coloring material, titanated mica, mica, talc, sericite, nylon powder, zinc stearate, etc. A non-aqueous cosmetic composition containing 29.9 to 39.9% by weight of a mixture of two or more of the materials is disclosed. Korean Patent Publication No. 10-2146522 relates to a natural preservative composition for cosmetics and a cosmetic composition containing the same, which is a multifunctional preservative having whitening, wrinkle reduction, and antioxidant functions. A natural preservative composition applicable to a product and a cosmetic composition containing the same are disclosed.

The present invention is to prevent the side effects of cosmetic use caused by the addition of conventional chemical preservatives, and contains extracts of natural herbs and complex ionic minerals, so that extracts of gosam and nut gall that have the effect of natural preservatives that can replace conventional chemical preservatives are effective. It is intended to provide a cosmetic preservative composition as a component and a manufacturing method thereof.

A cosmetic preservative composition containing gosam and nut gall extracts as active ingredients according to an embodiment of the present invention is subjected to hot water extraction by heating the selected and washed gosam raw materials at 120 ° C. at a pressure of 1.2 atm for 5 hours to obtain the gosam extract. Step (S100); Obtaining a nut gall extract by performing hot water extraction by heating the selected and washed gallbladder raw material at 120 ° C. at a pressure of 1.2 atm for 5 hours (S110); Alternatively, simultaneously proceeding with the obtaining gosam extract step (S100) and the gallbladder extract obtaining step (S110); Obtaining a saturated rock salt solution by putting the rock salt in a stirrer and dissolving it while stirring at low speed so that it is saturated in distilled water (S120); Obtaining a complex mineral solution obtained by diluting the complex mineral concentration 100 times or more to obtain an aqueous solution (S130); A first composite solution preparation step (S140) of mixing the solution and extract prepared in the steps S100 to S130 at a predetermined ratio and stirring for 24 hours or more; It may be composed of an impregnation step (S150) of mixing the composition of the first composite solution prepared in S140 with 0.2 to 1.0% by weight of the cosmetic composition and stirring in a stirrer to complete the cosmetic raw material.

In addition, the first composite solution preparation step (S140) may be a mixture of 80% by weight of complex ionic mineral solution, 5% by weight of sorghum extract, 5% by weight of gall nut extract, and 10% by weight of rock salt saturated solution.

According to Experimental Examples 1 to 5 of the present invention, the cosmetic preservative composition prepared by the method of the present invention has an effect that can be used as a cosmetic preservative composition without the addition of a separate preservative because the antibacterial ability against the strain is confirmed.

Figure 1 shows a schematic diagram of a method for preparing a cosmetic preservative composition containing the extracts of Gosam and gall nut of the present invention as active ingredients.
Figure 2 shows a schematic view of the step of obtaining the soybean extract extract according to Example 1 of the present invention.
3 shows the experimental results according to Experimental Example 3.
4 shows the experimental results according to Experimental Example 3.
5 shows the experimental results according to Experimental Example 3.
Figure 6 shows the antimicrobial activity test results of the cosmetic preservative composition according to Experimental Example 4.
Figure 7 shows the antimicrobial activity test results of the cosmetic preservative composition according to Experimental Example 4.
8 shows the results according to Experimental Example 5.
Figure 9 shows a cosmetic comprising a cosmetic preservative composition containing the extracts of Gosam and gall nut of the present invention as an active ingredient.

Hereinafter, a detailed description of a cosmetic preservative composition containing the extracts of Gosam and gall nut of the present invention as an active ingredient and a method for manufacturing the same with accompanying drawings will be described in detail.

Figure 1 shows a schematic diagram of a method for preparing a cosmetic preservative composition containing the extracts of Gosam and gall nut of the present invention as active ingredients. Obtaining a high ginseng extract by subjecting the selected and washed high ginseng raw material to hot water extraction by heating at 120° C. at a pressure of 1.2 atm for 5 hours (S100); Obtaining a nut gall extract by performing hot water extraction by heating the selected and washed gallbladder raw material at 120 ° C. at a pressure of 1.2 atm for 5 hours (S110); Alternatively, simultaneously proceeding with the obtaining gosam extract step (S100) and the gallbladder extract obtaining step (S110); Obtaining a saturated rock salt solution by putting the rock salt in a stirrer and dissolving it while stirring at low speed so that it is saturated in distilled water (S120); Obtaining a complex mineral solution obtained by diluting the complex mineral concentration 100 times or more to obtain an aqueous solution (S130); A first composite solution preparation step (S140) of mixing the solution and extract prepared in the steps S100 to S130 at a predetermined ratio and stirring for 24 hours or more; It may be composed of an impregnation step (S150) of mixing the first composite solution composition prepared in S140 with 0.2 to 1.0% by weight of the cosmetic composition and stirring in a stirrer to complete the cosmetic raw material.

The high ginseng extract obtaining step (S100) and gall nut extract obtaining step (S110); can be obtained by performing hot water extraction by heating the high ginseng and nut gall in the same ratio in a high-pressure water bath at 120 ° C. for 5 hours at a pressure of 1.2 atm. . Hereinafter, each step will be described with reference to an embodiment for a more detailed description.

<Example 1> Obtaining Gosam extract (S100)

Figure 2 shows a schematic view of the step of obtaining the soybean extract extract according to Example 1 of the present invention. The step of obtaining the high ginseng extract of the present invention includes a pretreatment step (A) of selecting and washing the high ginseng raw material and cutting it into a predetermined size; Extraction step (b) of extracting hot water from the goginseng that has gone through step (a); A filtration and concentration step (c) of filtering and concentrating the extract obtained through step (b); It may consist of a packaging step (D) of storing the high ginseng extract that has gone through step (c) at room temperature and storing it until the implementation of the step to be described later.

In the pretreatment step, it is preferable to select Korean ginseng, wash it three or more times in purified water, dry it naturally for 5 to 7 days, and then cut it into a size of less than 100 mm.

Gosam referred to in the present invention is a perennial herb belonging to the dicotyledonous plant Rosemary leguminous family, and is a wildflower that grows wild in mountainous areas throughout the country, and flowers bloom in stratified inflorescences. The scientific name of sophorae flavescens Aiton is Sophorae flavescens Aiton. The root is mainly used as a medicinal material, and it is said that it has the effects of killing wind, clearing heat and dampening, and treating leukopenia, anti-radiation, lowering blood sugar, anti-inflammatory It is used for tumor, antibacterial, and suppression of immune function, and in the private sector, decocted stems or leaves are used as insecticides and are effective in treating acne.

In the extraction step, hot water extraction is performed by putting purified water in an amount of 20 to 30 times the weight of the high ginseng that has undergone the pretreatment step into a pressure vessel and heating at 120 ° C. at a pressure of 1.2 atm for 5 hours. The hot water extract of sorghum ginseng after the extraction step includes a first filtration step of removing solids using a filter net and a second filtration step of filtering with a 100 μl cartridge filter. Thereafter, it is subdivided into 10L each by the room temperature packaging method and stored until the step to be described later.

<Example 2> Obtaining nut gall extract step (S110)

Figure 2 shows a schematic diagram of the gallbladder extract obtaining step according to Example 1 of the present invention. The gallbladder extract obtaining step according to Example 2 of the present invention can be carried out according to the same steps as the schematic diagram of the Gosam extract obtaining step according to Example 1 above.

In the pretreatment step, gallbladder is selected, washed, and dried at 60 ° C. The gallbladder referred to in the present invention refers to an insect gall made by parasitic on the leaves of a gallbladder of the aphids of the family Aphids of the family Arachnidaceae, and in oriental medicine, it has convergence, hemostasis, detoxification, and antibacterial It is an herbal medicine known to be effective and prescribed for diarrhea, prolapse, gastric ulcer, duodenal ulcer, cold sweat, oil well, bloody stool, hematuria, stomatitis, etc.

The dried nut gall raw material is uniformly pulverized, and hot water extraction is performed by adding purified water 20 to 30 times the weight of the gall nut and heating at 120 ° C. at a pressure of 1.2 atm for 5 hours. Extraction according to the embodiment of the present invention was carried out for 10 hours at 90-100 ℃.

As the extraction method, methods such as hot water extraction, cold brew extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, compression, etc. may be used, preferably through hot water extraction. In addition, the desired extract may be additionally subjected to a conventional fractionation process or may be purified using a conventional purification method.

Then, after the extraction step is completed, the concentrate prepared through the same filtration and concentration step as in Example 1 is subdivided into 10 L each, packed at room temperature, and stored until the step described later.

In addition, high ginseng extract obtaining step (S100) and nut gall extract obtaining step (S110); can be obtained by performing hot water extraction by heating for 5 hours at a pressure of 1.2 atm at 120 ° C. there is.

<Example 3> Obtaining a saturated rock salt solution (S120)

The saturated rock salt solution of the present invention is a step of preparing a saturated rock salt solution by putting rock salt in a stirrer and dissolving it while stirring at low speed so as to become saturated in distilled water. The rock salt referred to in the present invention is a rock crystal formed by evaporation of sea water or a salt lake, and the composition or content of impurities varies depending on the environment of the place where it was created, but the main component is, of course, sodium chloride (NaCl), and is mainly cube-shaped. It is a cubic mineral with crystals in shape.

The color is mostly colorless or white because of sodium chloride, but occasionally red, yellow or blue halite is also found, most likely due to impurities. The hardness expressed by the Mohs hardness scale is about 2 to 2.5, which is similar to that of gypsum, and the density is 2.168 g/cm 3 . Among them, Himalayan rock salt is salt that contains more than 30 beneficial minerals.

Beneficial minerals contained in Himalayan rock salt discharge skin wastes, have a cosmetic effect that makes skin elastic and soft, have a reducing power that restores substances to their original state against oxidizing power that corrupts substances, and have excellent antibacterial action, which prevents skin aging. It is known to turn damaged skin into elastic, healthy and beautiful skin through suppression, skin regeneration, and antibiotic action. In addition, Himalayan rock salt contains more than 30 kinds of minerals, and it is known that negative ions are released to have excellent effects on allergies and atopy.

The rock salt is pulverized using a grinder to make it pulverized and put into a stirrer. The size of the finely divided rock salt may be particles having a size of 50-150 mesh. The stirrer may be a low-speed stirrer capable of increasing the activity of the particle motion. At this time, the low-speed stirring speed may be 100-500 rpm. In addition, it is possible to obtain a stabilized saturated rock salt solution by introducing rock salt purified through a filtering process before being introduced into the stirrer into the stirrer. According to an embodiment of the present invention, 9 kg of distilled water was added to 1 kg of undifferentiated rock salt and stirred at a speed of 250 rpm for 3 hours to obtain a saturated rock salt solution.

<Example 4> Obtaining complex ionic mineral solution (S130)

In the step of obtaining the complex ionic mineral solution of the present invention, 0.5% by weight or more of citric acid, 0.1% by weight or more of L-glutamic acid, 560ppm or more of potassium, 3900ppm or more of calcium, and other complex minerals are mixed in appropriate amounts to obtain a concentration of 100 times or more of the mixed complex ionic minerals. It is a step to obtain an aqueous solution by dilution.

Complex minerals referred to in the present invention may include the complex minerals listed in Table 1 as citric acid, L-glutamic acid, potassium, calcium, and other complex minerals. Table 1 below shows the main components and contents of complex ionic minerals.

Main components and contents of complex ionic minerals ingredient name content citric acid 0.5% by weight or more L-glutamic acid 0.1% by weight or more potassium 560ppm or higher calcium 3900ppm or higher Other complex mineral content (ppm) Fe 12768 Cu 25 Mn 108 K 68 P 31 S 75,023 Mg 887 Al 8,709 Ca 440 Na 42 Si 53 Sr 8 Ti 41 V 69

Complex ion minerals are obtained by ion-mineralization of 10 kinds of minerals, such as citric acid, K, and Ca, through a water-solubility process. When used as an aqueous solution, it has water purification and deterioration inhibition, and when used as a cosmetic or wet tissue composition, it has antibacterial properties, virus prevention and suppression, and efficacy effects such as extension of shelf life.

The efficacy of the complex ion mineral is an antibacterial action that inhibits the growth of bacteria and fungi by preventing oxidation of organic matter and sterilization by osmotic action. It is a catalyst mineral that can be oxidized and reduced simultaneously. Anemia prevention that fundamentally prevents anemia by increasing blood oxygen concentration by promoting hemoglobin production with iron ions that have excellent binding ability to proteins, blood purification that purifies blood by reducing the amount of ammonia and carbon dioxide in the blood, etc. has the effect of

<Example 5> First composite solution preparation step (S140)

The first composite solution preparation step of the present invention is a step of mixing and stirring the sorghum extract, nut gall extract, rock salt saturated solution, and complex ionic mineral solution prepared according to Examples 1 to 4 at a predetermined ratio.

When preparing the first composite solution, it may be a first composite solution composition mixed with 80% by weight of complex ionic mineral solution, 5% by weight of sorghum extract, 5% by weight of gall nut extract, and 10% by weight of rock salt saturated solution. In addition, in the mixing and stirring step, the first composite solution composition may be put into a stirrer and stirred at room temperature for 24 hours or more.

<Example 6> Impregnation step (S150)

The impregnation step of the present invention consists of an impregnation step (S150) of mixing 0.2 to 1.0% by weight of the first composite solution prepared according to Example 5 in the cosmetic composition and stirring in a stirrer to complete the cosmetic raw material.

Usually, the cosmetic composition includes purified water, and the purified water includes 50 to 60% by weight in the case of cream and 30 to 40% by weight in the case of massage cream. After mixing and mixing 0.2 to 1.0% by weight of the primary composite solution, the cosmetic composition may be mixed.

<Experimental Example 1> Evaluation of antibacterial activity of Gosam extract according to the extraction method

An antibacterial activity test was performed using the hot water extract of Kosam obtained in Example 1 and the extract of Kosam extracted by the solvent extraction method of ethanol and methanol as comparative examples. Hot water extraction, ethanol 30%, 50% 70% extraction and methanol 30%, 50%, 70% extraction, and the test strains are Salmonella typhimurium ( S. typhimurium #2514), Vibrio anguillarum ( V. anguillarum V -FP-5228), Edwardsiella tarda ( E. tarda E-FP-5060), and Streptococcus iniae ( S. iniae S-FP-5028) were used, and Table 2 below shows the results.

The antibacterial activity was evaluated by measuring the diameter of the clear zone for each strain of bacteria generated from each extract by the agar well diffusion method, and among the values, the values in parentheses were calculated by converting the diameter of the clear zone to the amount of pefloxacin, an existing antibiotic. Relative amounts are indicated.

As a result of the antibacterial activity test, it was confirmed that all four strains of the sorghum extract extracted by the hot water extraction method had antibacterial activity, but the growth of the Streptococcus iniae strain was confirmed in the sorghum extract extracted with ethanol and methanol as comparative examples.

Antibacterial activity evaluation result of Gosam according to the extraction method test strain hot water extraction Ethanol extraction Methanol extraction 30% 50% 70% 30% 50% 70% S. typhimurium 0 0 0 0 0 0 0 V. anguillarum 0 0 0 0 0 0 0 E. tarda 0 0 0 0 0 0 0 S. iniae 0 1.5
(53.53) 1.8
(71.71) 1.7
(65.18) 0 1.7
(65.18) 1.7
(65.18) *The number is the diameter of the antibacterial active clear zone (unit: cm),
**Numbers in ( ) are converted to pefloxacin (unit: ug)

<Experimental Example 2> Evaluation of antibacterial activity of gall nut according to extraction method

The gallbladder hot water extract obtained according to Example 2 and the gallbladder extract extracted by the solvent extraction method of ethanol and methanol were used as comparative examples to test antibacterial activity, and the method was carried out in the same manner as in Experimental Example 1. Table 3 below shows the results.

Antibacterial activity evaluation result of gall nut according to extraction method test strain hot water extraction Ethanol extraction Methanol extraction 30% 50% 70% 30% 50% 70% S. typhimurium 0 0 0 1.5
(3.64) 0 1.5
(3.64) 1.5
(3.64) V. anguillarum 1.5
(8.37) 2.1
(19.57) 1.8
(12.56) 1.75
(11.72) 2.4
(33.64) 2.4
(33.64) 2.7
(76.30) E. tarda 0 0 0 1.6
(34.92) 0 0 0 S. iniae 1.5
(8.37) 1.9
(78.78) 2.0
(86.46) 3.0
(228.42) 1.6
(50.14) 2.0
(86.46) 2.8
(184.87) *The number is the diameter of the antibacterial active clear zone (unit: cm),
**Numbers in ( ) are converted to pefloxacin (unit: ug)

As a result of the antibacterial activity test, the nut gall extract extracted by the hot water extraction method was able to confirm the strains of V. anguillarum and S. iniae , and the growth of all strains was confirmed in the nut gall extract extracted by the 70% ethanol extraction method, and extracted by the methanol extraction method. The extracted gallbladder extract can confirm the growth of strains other than E. tarda , so it was confirmed that the gallbladder extract extracted by hot water extraction has a strain killing effect compared to the gallbladder extract according to other extraction methods, but it was confirmed that the killing of all strains was difficult. .

As described above, the effect of natural preservatives was examined using the extracts of high ginseng and gall nut, which are stable and antibacterial to the human body among medicinal herbs.

<Experimental Example 3> Evaluation of antibacterial activity according to the mixed composition

Experimental group 1 (ion mineral + gosam extract) and experiment group 2 (ion mineral + gosam extract) by varying the mixing type of the gosam extract and nut gall extract prepared according to Examples 1 and 2 and the complex ion mineral solution prepared according to Example 4 + gallbladder extract), experimental group 3 (ion mineral + nut gall extract) was prepared.

The prepared test group was diluted in 1000 g of distilled water to form an aqueous solution, and then a phosphate buffer was added so that it was 10 times the weight of the test group. After standing for 30 minutes, 1 ml of trypticase soy agar was added to the plate medium. After inoculation of Eschericahia Coli, Pseudomonas aeruginosa, and Staphylococcus as test strains at a concentration of 10 cfu/g, incubation was performed in an incubator at 35±1° C. for 48 hours. After culturing, while keeping the medium at room temperature, bacterial growth, properties, and flavor were observed on the same day, 3rd day, 7th day, 14th day, and 21st day. Table 4 below shows the results according to Experimental Example 3 of the present invention, and FIGS. 3 to 5 show the experimental results according to Experimental Example 3.

As a result, it was confirmed that experimental groups 1 and 2 had excellent antibacterial properties because there was no microbial growth, but experimental group 3 confirmed the growth of microorganisms over time, and the mixed solution of nut gall extract and complex ion mineral solution was found to have no antibacterial activity. It is foreseeable.

Antibacterial activity evaluation result according to mixed composition date division Experimental group 1 (ion mineral + gosam) Experimental group 2 (ion mineral + gosam + nut gall) Experimental group 3 (ion mineral + nut gall) first
(2021.2.19.) appearance fitness fitness fitness dreadlocks fitness fitness fitness pH Semi-manufactures 8.7 8.45 5.27 Finished 7.32 7.38 6.98 microbe Germ <10 cfu/g <10 cfu/g 10 cfu fungi 10 cfu <10 cfu/g 20cfu Day 3
(2021.2.22.) appearance fitness fitness fitness dreadlocks fitness fitness fitness pH Finished 6.96 6.87 6.85 microbe Germ <10 cfu/g <10 cfu/g 180 cfu fungi <10 cfu/g <10 cfu/g <10 cfu/g Day 7
(2021.2.26.) appearance fitness fitness fitness dreadlocks fitness fitness fitness pH Finished 6.66 6.61 6.25 microbe Germ <10 cfu/g <10 cfu/g <10 cfu/g fungus <10 cfu/g <10 cfu/g tntc Day 14
(2021.3.05.) appearance fitness fitness mold growth dreadlocks fitness fitness fitness pH Finished 6.65 6.55 6.36 microbe Germ <10 cfu/g <10 cfu/g tntc fungus <10 cfu/g <10 cfu/g tntc Day 21
(2021.3.12.) appearance fitness fitness fitness dreadlocks fitness fitness fitness pH Finished 6.32 6.53 6.74 microbe Germ <10 cfu/g <10 cfu/g 50 cfu fungi <10 cfu/g <10 cfu/g 70 cfu

<Experimental Example 4> Antibacterial activity test of cosmetic preservative composition containing gosam and gall nut extract as an active ingredient

An antibacterial test was conducted on the cosmetic preservative composition containing the extracts of gosam and gall nut prepared according to Examples 1 to 7 of the present invention as an active ingredient. In order to confirm the antimicrobial properties of the cosmetic preservative composition of the present invention, a wet tissue 5X5cm containing the above-prepared ginseng and gall nut extract and a Stonacher film 5X5cm were prepared as a control, diluted in 1000g of distilled water to form an aqueous solution, and then administered to the wet tissue Then, phosphate buffer was added so that the water absorbed from the wet tissue was 10 times the weight of the sample taken, left for 30 minutes, then 1 ml was added to the trypticase soy agar plate medium, and E. coli as the test strain ( Eschericahia Coli) 3.2X10 ⁵ CPU/mL, Pseudomonas aeruginosa 1.9X10 ⁵ CPU/mL, Staphylococcus 3.9X10 ⁵ CPU/mL After inoculation, after culturing in an incubator at 35±1℃ for 48 hours, bacterial growth observed. The strains immediately after and 5 minutes after the introduction of the above strains were measured.

6 to 7 show the antibacterial activity test results of the extract of gosam and gall nut according to Experimental Example 4 of the present invention. As all the bacteria were killed 5 minutes after the strain was added, the present invention confirmed that the antibacterial properties of the extracts of Gosam and gall nut were very excellent because there was no proliferation of bacteria.

<Experimental Example 5> Antibacterial activity test of the first composite solution composition

An antibacterial test was conducted on the first composite solution composition prepared according to Example 6 of the present invention. The first composite solution composition prepared according to Example 6 of the present invention was diluted in 1000 g of distilled water to form an aqueous solution, and then administered to a wet tissue so that the absorbed water of the wet tissue was 10 times the weight of the sample collected. After adding phosphate buffer and standing for 30 minutes, add 1 ml to trypticase soy agar plate medium, and then inoculate Eschericahia Coli and Staphylococcus as test strains at a concentration of 10 cfu/ml After culturing for 48 hours in an incubator at 35 ± 1 ° C., bacterial growth was observed. The strains immediately after and 5 minutes after the introduction of the above strains were measured.

8 shows the results according to Experimental Example 5 of the present invention. Since all the bacteria were killed 5 minutes after the strain was introduced, it can be seen from the above experimental results that the present invention has excellent antibacterial properties because there is no proliferation of bacteria.

Figure 9 shows a cream cosmetic comprising a cosmetic preservative composition containing the extracts of Gosam and gall nut of the present invention as an active ingredient.

The present invention provides a cosmetic preservative composition with natural preservative efficacy that can replace existing chemical preservatives in line with the needs of modern people who are environmentally friendly and sensitive to side effects, thereby meeting the needs of consumers and creating profits for related industries. It can be added to, so it has industrial applicability.

Claims (3)

Obtaining an extract by performing hot water extraction by mixing and heating the selected and washed high ginseng raw material and the selected and washed gallbladder raw material at a ratio of 1: 1 (A);
Obtaining a saturated rock salt solution by putting the rock salt in a stirrer and dissolving it while stirring at low speed so that it is saturated in distilled water (B);
Obtaining a complex ionic mineral solution obtained by diluting the concentration of the complex ionic mineral by 100 times or more to obtain an aqueous solution (C);
A first composite solution preparation step of mixing the solutions and extracts prepared in the steps (A), (B) and (C) at a predetermined ratio and stirring for 24 hours or more;
Method for producing a cosmetic preservative composition comprising gosam and gall nut extract as an active ingredient, characterized in that consisting of an impregnation step of mixing 0.2 to 1.0% by weight of the composition of the first composite solution in the cosmetic composition to complete the cosmetic raw material The method of claim 1, wherein the first composite solution preparation step is 80% by weight of complex ionic mineral solution, 10% by weight of mixed extract of gosam and nut gall, and 10% by weight of rock salt saturated solution. Manufacturing method of cosmetic preservative composition containing as an active ingredient A cosmetic preservative composition characterized in that it is mixed with 80% by weight of complex ionic mineral solution, 10% by weight of mixed extract of gosam and gall nut, and 10% by weight of rock salt saturated solution

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