KR101841976B1 - Antifungal and antibacterial composition comprising herbal extract - Google Patents

Abstract

The present invention relates to an antifungal and antibacterial composition containing a plant extract. More specifically, the present invention relates to a composition exhibiting excellent antifungal effects and antibacterial functions by containing lonicer aflower, gluconic lactone, and Phellinus linteus. According to the present invention, the composition ensures excellent sterilizing functions, fungicidal functions, antibacterial functions, and antifungal effects against bacteria, prevents skin dryness, and exhibits anti-inflammatory effects without irritating skin by using a natural herbal medicine, thereby preventing various inflammatory skin diseases. Especially, the composition can be used as a female cleanser to provide effects of cleansing the vagina on pudenda, reducing leukorrhea and itchiness, removing odors, and alleviating vaginitis.

Description

TECHNICAL FIELD [0001] The present invention relates to an antifungal and antimicrobial composition containing a plant extract,

The present invention relates to an antifungal agent and an antimicrobial composition containing a plant extract, and more particularly, to a composition capable of exhibiting excellent antifungal effect and antimicrobial effect by including gum eu, gluconolactone, and mushroom.

Skin is a part of the body that is directly exposed to the external environment. It acts as a protective layer to protect important organs of our body, as well as controlling water evaporation and protecting the body from external infections.

The female vagina is the connection between the vulva and the uterus. The vaginal acidity is maintained at pH 3.2 by lactic acid secretion by beneficial microorganisms in the vagina, thereby inhibiting or killing most of the harmful bacteria. Because vagina is the most humid and warm place in a woman's body, when the vagina inside is outside the normal pH range, it becomes an environment suitable for bacteria or fungus and it causes vaginitis. Vaginitis is a female disease that women experience once in their lifetime. Trichomoniasis, which causes itching, and Candida, which causes a sudden and large amount of vaginal discharge, are among the causes of vaginitis.

Therefore, when the soap is used frequently, the vagina can be changed to alkaline to increase the risk of infection, rather than to live in an environment where normal bacteria can not live, and the irritation component of the alkali can increase the occurrence of allergy.

Accordingly, there is a need for a detergent exclusively for vaginal substances derived from natural products which can alleviate irritation while suppressing the proliferation and killing of harmful bacteria with a low acidity and pH balance of the vaginal pH balance and less allergy and side effects.

Korean Patent No. 10-1168225

It is an object of the present invention to provide a composition which is eco-friendly as natural oriental oriental preparations, has no skin irritation, is excellent in fungicidal power and sterilizing power, and can prevent inflammation due to antifungal and antibacterial effects.

In order to achieve the above object, the present invention provides an antifungal and an antimicrobial composition comprising ginseng, gluconolactone and mushroom extract as an active ingredient.

Gold silver

In the present invention, Lonicera japonica Thunb . Is a flower bud or a flower bud of an indigo vine belonging to the genus lonicera (Caprifoliaceae). Ingredients of gingkohwa are essential oil, chlorogenic acid, flavones, iridoids, saponins, luteolin and so on.

Gluconolactone

In the present invention, gluconolactone (C ₅ H ₁₀ O ₆ ) is a lactone component of gluconic acid, and is one of polyhydroxy acids (PHA), similar to alpha-hydroxy acid (AHA) It has the same function but it has a larger size of gluconolactone molecule than AHA, so it is not easily absorbed to the skin and has less irritation and has antibacterial power. Therefore, it is used as a natural preservative by blending with cosmetic composition, It shows preservative effect and gives perfume to cosmetics.

Situation mushroom

In the present invention, the mushroom (Mulberry, Phellinus linteus ), also known as woody mud mushroom, contains a large amount of beta-glucan polysaccharide. The beta-glucan exhibits an antimicrobial effect by activating macrophages, and exhibits an immunomodulatory function and an immune function-enhancing effect that improve immunity when the immunity is decreased and suppress immunity when the immunity is excessive.

The composition may comprise 0.3 to 4.0% by weight of ginger extract, 0.5 to 2.0% by weight of gluconolactone, and 0.5 to 2.0% by weight of mushroom extract, based on the total weight of the composition.

More preferably, it may contain 2.0 to 3.0% by weight of ginger extract, 0.8 to 1.2% by weight of gluconolactone and 0.8 to 1.2% by weight of mushroom extract.

The expected effect can be realized when the above range is maintained.

The extract of the present invention can be obtained by using an extract obtained by an extraction treatment, a diluted or concentrated liquid of the extracted liquid, a dried material obtained by drying the extracted liquid, a controlled preparation or a purified product of the extracted liquid, Lt; RTI ID = 0.0 > of all formable < / RTI > formulations. In the present invention, it is most preferable that the extract is prepared in the form of a dry powder after extraction.

The glycyrrhizin, gluconolactone and mushroom extract may be extracted with at least one extraction solvent selected from the group consisting of water, anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and butylene glycol.

The composition may be a cosmetic composition.

The composition may be applied as a cleaning composition.

The cleaning composition may be used for female cleaners.

When the antifungal and antimicrobial composition of the present invention is used as a cleaning composition, it may contain isopropanol, isopropyl alcohol, ethanol, hydrogen peroxide, benzalkonium chloride, cresol and the like which are used as conventional cleaning components. , Liquid type, and the like.

The composition according to the present invention is excellent in antibacterial activity, antibacterial activity, fungicidal activity and sterilizing power against bacteria, and can prevent dryness of the skin. Also, it is free from skin irritation by using natural herbal preparation, Inflammatory skin diseases can be prevented.

Especially, it can be used as a female cleanser to provide the effect of cleansing the vulva, cooling, itching, removing odors, and improving vaginitis.

FIG. 1 shows the results of confirming antimicrobial activity against noxious bacteria in the extracts of Ganoderma lucidum, gluconolactone, mushroom extract, and mixtures thereof.
Fig. 2 shows the result of the experiment of the antifungal and antimicrobial composition according to Example 1 for Escherichia coli and Staphylococcus aureus. Fig. 2 (a) shows the state immediately after inoculation of the bacteria into petri dish, Fig. 2 (C) shows the state in which the bacteria were inoculated after 5 minutes, and (d) shows the state in which the bacteria were inoculated after 10 minutes.
FIG. 3 shows the results of examining the antifungal activities of Gingko nuts extract, gluconolactone, mushroom extract, and the mixture thereof.

Hereinafter, the present invention will be described in more detail with reference to Examples. The objects, features and advantages of the present invention will be readily understood through the following examples. The present invention is not limited to the embodiments described herein, but may be embodied in other forms. The embodiments described herein are provided to enable those skilled in the art to fully understand the spirit of the present invention. Therefore, the present invention should not be limited by the following examples.

Example  : Preparation of antifungal and antibacterial composition

Preparation of extract

To 1 g of washed gold europaea, 5 ℓ of 80% ethanol aqueous solution was added and the mixture was refluxed three times and then immersed at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation. The separated filtrate was concentrated under reduced pressure, and the resulting extract was suspended in water. The filtrate was extracted five times with 1 liter of ether to remove the pigment. And extracted three times with 500 ml. The total 1-butanol layer thus obtained was concentrated under reduced pressure to obtain a 1-butanol extract. The extract was dissolved in a small amount of methanol, and a large amount of ethyl acetate was added thereto. The resultant precipitate was dried to obtain 200 g of Ganoderma lucidum extract. .

The mushroom extract was purchased from dermalab. However, it is also possible to prepare and use the mushroom extract by applying the same method as the method for manufacturing the mushroom extract.

Gluconolactone was supplied by Risell Labs.

Preparation of antifungal and antimicrobial compositions

Composite compositions were prepared by mixing the components listed in Table 1 below.

Example (Unit: wt%) ingredient Example One 2 3 Active ingredient
(extract) Gold silver 3.0 2.0 1.0 Gluconolactone 1.0 1.0 1.0 Situation mushroom 1.0 1.0 1.0 Butylene glycol 5.0 5.0 5.0 Purified water to 100 to 100 to 100

Experimental Example 1: Measurement of antimicrobial activity

The disk diffusion method was used to confirm the antimicrobial activity of the composition according to the present invention. Bacillus subtilis (KCTC1914), known as harmful bacteria, Pseudomonas putida (KCTC2198), Staphylococcus aureus (KACC10196). Staphylococcus epidermidis (CCARM3710), Staphylococcus aureus (CCARM3091), Streptococcus mutans (KACT3298), and Streptococcus mutans (KCTC3306) were plated on a plate medium for disk diffusion assay. After the sterilized paper disk was attached to a plate culture medium on which major noxious bacteria had been streaked, the composition of Example 1, in which the ginger extract, gluconolactone, mushroom extract and the above three extracts were mixed, was dissolved in dimethylsulfoxide (DMSO) The sample was dispensed onto a sterilized paper disk at a concentration of 1 mg / disc and placed on a medium. The size of the growth zone (Clean Zone) was measured after culturing the plate medium for 24 hours at an appropriate temperature according to each strain. The results are shown in Fig.

As shown in FIG. 1, it was confirmed that the growth inhibition of growth of the paper disk on which the composition according to Example 1 was dropped among the four paper disks was the largest, and it was confirmed that the antimicrobial activity was excellent.

Experimental Example 2: Measurement of bactericidal activity

E. coli KCTC 2571 and Staphylococcus aureus KCTC 2513 were cultured in Petri dishes at 32 ° C for 48 hours in order to confirm the bactericidal activity of the composition according to the present invention. The initial bacterial counts of cultured bacteria were 2.57 × 10 ⁴ CFU / mL for E. coli and 3.67 × 10 ⁵ CFU / mL for S. aureus. The test bacteria were prepared by diluting the previously cultured bacteria with 9 mL of sterilized physiological saline to obtain a final bacterial count of 10 ³ CFU / mL. Thereafter, 100 g of the antimicrobial composition according to the Example, sterilized physiological saline, and 1 mL of the test solution were added to the two vials to prepare a mixed sample. 1 mL of the prepared sample is taken in 9 mL of letheen broth and diluted to 10 ² CFU / mL. The diluted bacteria are dispensed into a freshly prepared Petri dish and then 20 mL of the test medium is poured Mixed and gelled. After 1 minute, take 1 mL of sample in 9 mL letheen broth, dispense 1 mL in Petri dish, pour 20 mL of test medium, mix and gel, repeat for 3, 5 and 10 minutes And sterilization status of the bacteria was observed.

As a result of the experiment, it was confirmed that the composition of Example 1 had the best sterilizing power as shown in Table 2 (FIG. 2).

Identification of the sterilization power of the antimicrobial composition Viable cell count
Test bacteria Aerobic plate count (CFU / mL) after Remarks Immediately 3 minutes 5 minutes 10 minutes Escherichia coli 1.88 × 10 ⁴ 0 0 0 Example 1 Staphylococcus 2.25 x 10 ⁵ 1.80 x 10 ³ 0 0 Escherichia coli 1.80 x 10 ⁴ 2.60 x 10 ² 5.8 x 10 0 Example 2 Staphylococcus 2.22 x 10 ⁵ 4.80 × 10 ³ 0 0 Escherichia coli 1.80 x 10 ⁴ 2.78 × 10 ² 1.6 x 10 0 Example 3 Staphylococcus 2.22 x 10 ⁵ 1.60 x 10 ³ 1.2 x 10 0

As a result, it was confirmed that the higher the content of the gingkoff extract, the better the sterilizing power of the composition.

Experimental Example 3: Measurement of antifungal activity

In order to confirm the antifungal activity of the composition according to the present invention, the disc diffusion method was carried out in the same manner as in Experimental Example 1 using Aspergillus niger ATCC 16404, the causative fungus of vaginitis, to measure growth inhibition. Each composition was spotted at a concentration of 1 mg / disc on sterilized paper disks.

As a result, as shown in FIG. 3, it was confirmed that the growth inhibition of the growth of the paper disk with the composition according to Example 1 was the greatest, and the antifungal activity was excellent.

Identify antifungal activity by composition ingredient Growth restriction (mm) control 0 Gold silver 20 Gluconolactone 10 Situation mushroom 14 Eunhwa + Gluconolactone + Situ mushroom 27

Experimental Example 4: Evaluation of moisturizing effect

The skin moisturizing effect was evaluated in 20 to 30 years old male and 10 female. In the experiment, the cosmetic prepared from the composition prepared according to Examples 1 to 3 was applied to the hands for 15 days, 10 times a day, and the moisturizing condition of the skin was sensually evaluated. The results are shown in Table 4 below .

Check the moisture of the composition Example 1 Example 2 Example 3 ○ △ △ ※ Moisture power evaluation standard
- ○: Moisture is very good and moisturization is continued.
- △: Normal humidity
- X: No moisture, pulling phenomenon

As a result of the experiment, it was confirmed that the moisture resistance of Example 1 was the best.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereto will be. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (7)

As antifungal and antibacterial compositions,
Gingerol extract, gluconolactone and mushroom extract as active ingredients,
0.3 to 4.0% by weight of ginger extract, 0.5 to 2.0% by weight of gluconolactone, and 0.5 to 2.0% by weight of mushroom extract, based on the total weight of the composition,
An antifungal and antimicrobial composition for inhibiting Aspergillus niger .
delete The method according to claim 1,
Wherein the composition comprises 2.0 to 3.0% by weight of a ginger extract, 0.8 to 1.2% by weight of gluconolactone and 0.8 to 1.2% by weight of a mushroom extract, based on the total weight of the composition.
The method according to claim 1,
The gingival extract, gluconolactone and mushroom extract are extracted with at least one extraction solvent selected from the group consisting of water, anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and butylene glycol And an antimicrobial composition.
The method according to claim 1,
Wherein said composition is a cosmetic composition.
The method of claim 5,
Wherein the cosmetic composition is used as a cleaning composition.
The method of claim 6,
Wherein the cleaning composition is for a female cleanser.

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