KR102292487B1 - Composition for feminine hygiene care containing Copaiba resin extract - Google Patents

Abstract

The present invention relates to a composition for feminine hygiene care containing a copaiba resin extract as an active ingredient, and specifically, to a composition, which contains natural oil as an active ingredient to be safe and is applied for external uses to be used for prevention or treatment of female diseases or as a feminine cleanser.

Description

Composition for feminine hygiene care containing Copaiba resin extract

The present invention relates to a composition for feminine hygiene management comprising Copaiba resin extract as an active ingredient, and specifically, it contains natural oil as an active ingredient and is safe, and is applied externally to prevent or treat female diseases or as a feminine cleanser. It relates to a composition that can be used.

A woman's vagina is the connection between the vulva and the uterus, and is the most humid and warm place in a woman's body. Since the vagina of a woman is an environment suitable for bacteria and fungi, the main cause of vaginitis, there is a high possibility of developing diseases. In addition, the groin, which is also called the Y zone, is humid and warm, so if ventilation is insufficient due to women's underwear or clothes that are not comfortable to move, bacterial infection or inflammation is easy to occur.

Vaginitis is reported to include bacterial vaginosis, Candida vaginitis, Trichomonas vaginitis, and atrophic vaginitis, and the most common disease is bacterial vaginosis, Gardnerella vaginalis. (Gardnerella vaginalis), which is common along with bacterial vaginosis, is caused by the fungus Candida albicans.

Various antibiotics, fungicides, protozoa, anti-inflammatory drugs, etc. have been proposed to treat female diseases such as vaginitis or groin inflammation, but there is a risk of resistance and irritation, so there is a limit to freely use.

Povidone, glucoester, and glucoflavonoid ingredients can be used as feminine cleanser, but they have a disadvantage in that they have a strong odor or are cumbersome to use because they have to be washed with water after use.

Accordingly, the present inventors have devised a safe and convenient composition for feminine hygiene management containing natural ingredients.

One aspect provides a composition for feminine hygiene management, comprising an extract of copaiba, and argan oil as active ingredients.

Another aspect provides a feminine cleanser comprising a copaiba extract, and argan oil as active ingredients.

Another aspect provides a composition for treating or preventing female diseases, comprising the extract of Copaiba, and argan oil as active ingredients.

One aspect provides a composition for feminine hygiene management, comprising a copaiba extract, and argan oil as active ingredients.

The term “for female hygiene management” means a female cleanser or for treatment or prevention of female diseases that may be caused by negligence in female hygiene management. The female disease may mean vaginitis or groin inflammation.

The term “comprising as an active ingredient” may mean containing an effective amount that can exhibit a feminine cleansing effect, for example, antibacterial, anti-inflammatory, itching, pain, and the like.

Copaiba is extracted from the resin of the Copaiba tree, and has anti-inflammatory, analgesic and skin soothing effects. The Copaiba extract contains 80 w/w% or more of cannabinoids, and the cannabinoids may be specifically beta-cariofilen. The Copaiba extract may be an essential oil by steam distillation.

Argan oil is an oil component extracted from the seeds of argan fruit, and contains a large amount of squalene, which has excellent effects of removing toxins, moisturizing the skin, regenerating and anti-inflammatory.

The Copaiba extract and argan oil each have a certain amount of moisturizing, anti-inflammatory and antibacterial effects, but surprisingly, it was confirmed through antibacterial and anti-inflammatory tests that remarkably excellent moisturizing, anti-inflammatory and antibacterial effects can be obtained when they are mixed.

Copaiba extract and argan oil in the composition may be included in a weight ratio of 1: 1 to 2 as an extract having a purity of 90% or more, preferably 99% or more.

The composition includes evening primrose seed, lavender, tea tree, clary sage, Frankincense, Myrrha, Neroli, rose, geranium, ylang ylang as additional active ingredients. (Ylang Ylang), jasmine, jojoba, acacia flower, chamomile, vetiver, or an extract of a combination thereof may be further included.

The evening primrose extract is a seed oil obtained by extracting oil components from the seeds of evening primrose, and may exhibit anti-inflammatory effects in skin moisturizing and regeneration as well as female diseases.

The jojoba oil is an oil component extracted from seeds of jojoba fruit, and may exhibit moisturizing and anti-inflammatory effects.

The lavender, clary sage, neroli, rose, geranium, ylang-ylang, jasmine, acacia flower, and chamomile may be an essential oil extracted by steam distillation of flowers or floral water obtained by separating only water-soluble substances from the essential oil.

The tea tree is an extract of leaves and stems, and may be an essential oil by steam distillation.

The vetiver is an extract of the root, and may be an essential oil by steam distillation.

The frankincense is a resin extract of a Boswellia Carteri tree, and may be an essential oil by steam distillation.

The myrrh is a resin extract of Commiphora Myrrha, and may be a juiced oil or an essential oil by steam distillation.

The additional active ingredient has a skin soothing and inflammatory effect, and may further enhance the moisturizing, antibacterial, and anti-inflammatory effects of copaiba and argan oil.

In one embodiment, the composition may include argan oil, jojoba oil, evening primrose oil, copaiba essential oil, and lavender essential oil.

When the composition for feminine hygiene management includes an additional active ingredient, 6 parts by weight of a mixture of argan oil, jojoba oil, and evening primrose seed oil; and 4 parts by weight of a mixture of copaiba essential oil and lavender essential oil. Here, argan oil, jojoba oil, and evening primrose seed oil may be included in 1:1:1 parts by weight, and copaiba essential oil and lavender essential oil may be included in 1:1 parts by weight. The component ratio of each active ingredient may be important not only for efficacy, but also for feeling of use, appropriate volatility, and formulation.

In the composition for feminine hygiene management, each natural product is included as an extract. The term “extract” refers to a solvent in which the active ingredient contained in the extraction raw material is transferred by contacting the solvent and the extraction raw material under specific conditions. All can be included regardless. For example, extracting a component soluble in a solvent from a natural product using water or an organic solvent, a specific component of a natural product, for example, extracting only a specific component such as oil, etc. may be included. The extract may be extracted with one or more solvents selected from the group consisting of purified water, lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol, and the alcohol is 60 to 90 % concentration (v/v) of ethanol. When the natural product is extracted with an essential oil, it can be extracted using a commonly known method such as a distillation method, a pressing method, an enflame method, a immersion method, a solvent extraction method, and the like. The natural products may be specifically included in the composition as a base oil, essential oil, or water extract.

The composition for feminine hygiene management is formulated with one or more selected from the group consisting of softening lotion, astringent lotion, nutrient lotion, lotion, nutrient cream, massage cream, essence, cleansing cream, cleansing foam, cleansing water, pack, spray, and powder. can be pissed off

In the composition for feminine hygiene care, the mixture of the extracts may be included in an amount of 5 parts by weight or more, preferably 5 to 30 parts by weight, more preferably 8 to 12 parts by weight based on 100 parts by weight of the total composition. If the mixture of the extracts is less than 5 parts by weight in the total composition, the medicinal effect of the active ingredient may not be sufficiently expressed, and if it exceeds 30 parts by weight, it may rather irritate the skin.

The composition for feminine hygiene management may be used as an external preparation or for shunting. The term “external use” refers to direct application to the affected skin, and the term “jwahun” refers to applying to the skin by adding an appropriate amount to hot water and then evaporating the active ingredient with water vapor. The composition for feminine hygiene management may include an appropriate solvent to facilitate absorption of the active ingredient when used for external use or for shunting, volatilize appropriately so that the medicinal effect can be fermented to the application site and the surrounding area, and the solvent is propanediol , butylene glycol, 1,2-hexanediol, ethylhexyl glycerin, panthenol, purified water, or a combination thereof.

In the composition for feminine hygiene care, in each formulation, other components may be suitably blended within the range that does not impair the purpose according to the present invention, depending on the type or purpose of use, in addition to the essential components in each formulation.

The composition for feminine hygiene care may include a conventionally acceptable carrier, for example, oil, water, surfactant, humectant, lower alcohol, thickener, chelating agent, colorant, preservative, fragrance, etc. may be appropriately formulated, but limited thereto it's not going to be

The acceptable carrier may vary depending on the formulation. For example, when formulated into an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or their Mixtures may be used.

When the composition for feminine hygiene care is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or mixtures thereof may be used as a carrier component, and in the case of spray, chlorophyll It may further comprise a propellant such as fluorohydrocarbon, propane, butane or dimethyl ether.

When the composition for feminine hygiene care is formulated as a solution or emulsion, a solvent, solubilizer, or emulsifier may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, Propylene glycol, 1,3-butylglycol oil may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, fatty acid esters of polyethylene glycol or sorbitan may be used. can

When the composition for feminine hygiene care is formulated as a suspension, as a carrier component, such as water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters Suspending agents, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like can be used.

When the composition for feminine hygiene care is formulated as a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionates, lanolin derivatives, fatty alcohols, vegetable oils, glycerol, sugar and the like may be used.

The composition for feminine hygiene management is a fatty substance, an organic solvent, a solubilizer, a thickening agent, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, and a foaming agent commonly used in the industry according to the quality or function of the final product. ), fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, common in cosmetics It may additionally contain adjuvants commonly used in the field of cosmetics or dermatology, such as any other ingredients used as

However, the adjuvant and its mixing ratio may be appropriately selected so as not to affect the desirable properties of the feminine cleanser composition according to the present invention.

The composition for feminine hygiene management according to an aspect is convenient to use because it uses natural ingredients that are safe for the skin as an active ingredient, and has excellent antibacterial, anti-inflammatory, and moisturizing effects, effectively removing bacteria that cause vaginitis or groin inflammation, , is excellent in alleviating symptoms of vaginitis such as cold, itching, and bad odor.

In addition, the composition for feminine hygiene management according to an aspect is convenient to use because it can be directly applied to the skin, and it can be used in the same way as fumigation or shunting. less reluctance to use.

Hereinafter, the present invention will be described in more detail based on the following examples, but these are only for illustrating the present invention and the scope of the present invention is not limited in any way by these.

Preparation Example 1: Preparation of a composition for feminine hygiene management

Copaiba essential oil, argan seed oil, jojoba oil, evening primrose seed oil, and lavender essential oil were all 99.9% pure.

10 parts by weight of propanediol, 10 parts by weight of butylene glycol, 10 parts by weight of 1,2-hexanediol, 10 parts by weight of ethylhexylglycerin, 1 part by weight of panthenol, and _{0.01 parts by weight of EDTA-Na 2 were} mixed, and purified water was added thereto. to prepare a solvent mixture so as to be a total of 100 parts by weight. A total of 100 g of the example composition was prepared by mixing natural oil with the composition shown in Table 1 below with the prepared solvent mixture.

(g) Example comparative example One 2 One 2 3 4 5 6 7 Copaiba Resin 2 4 4 - 4 4 - - - lavender 2 - - 4 - - 4 4 4 argan seeds 2 6 - 2 - - 6 3 - jojoba seeds 2 - 3 2 6 - - - 3 Evening Primrose Seeds 2 - 3 2 - 6 - 3 3

Test Example 1: Cell safety evaluation

In order to evaluate the safety when the composition of the present invention is applied to the skin, a cytotoxicity test was performed on human skin fibroblasts.

Specifically, HDF (Human Dermal Fibroblast) cells were dispensed in a 96-well plate at ^{a concentration of 1×10 4} cells/well, and incubated at 37° C., 5% CO ₂ for 24 hours, and each prepared in Preparation Example 1 In Examples and Comparative Examples, cells were treated at concentrations of 100, 50, and 25 μg/μl and incubated again for 24 hours. Then, the medium was removed and treated with MTT{3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide} at a concentration of 5 mg/mL, and at 37°C, 5% CO ₂ for 2 hours. After incubation, 100 μl of DMSO (dimethyl sulfoxide) was added, and the absorbance was measured at 540 nm after stirring for 10 minutes.

As a result of the measurement, cytotoxicity was not observed at all concentrations of all tested Examples and Comparative Examples.

Test Example 2: Evaluation of antibacterial activity

In order to evaluate the antimicrobial activity against Candida albicans and Gardnerella vaginalis, which are bacteria that cause vaginitis, one of the female diseases, a disk diffusion method was performed.

Specifically, Candida albicans and Gardnerella vaginalis were plated by dispensing 100 μl each on a plate medium for disc diffusion measurement (Sabouraud Dextrose Agar. Difco. USA). Then, the Example and Comparative Example compositions prepared in Preparation Example 1 were dripped onto a sterilized 8mm paper disk at a concentration of 5mg/disc and placed on the medium.

As a positive control, itraconazol was used for Candida albicans, and Ampicillin was used for Gardnerella vaginalis, and each was used at a concentration of 100 μg/disk.

After treatment with each Example and drug, the plate medium was cultured at 28 ° C. for 48 hours, and then the size (mm) of the growth inhibition ring (Clean Zone) was measured, and the results are shown in Table 2 below.

(mm) candida albicans Gardnerella Vaginalis positive control 36 35 Example 1 31 32 Example 2 27 26 Comparative Example 1 19 19 Comparative Example 2 20 20 Comparative Example 3 19 21 Comparative Example 4 15 14 Comparative Example 5 13 16 Comparative Example 6 14 16 Comparative Example 7 10 11

As a result, it was confirmed that Examples 1 and 2 made a significantly wider growth-inhibiting ring compared to Comparative Examples 1 to 7 and had excellent antibacterial activity.

Test Example 3: Anti-inflammatory effect evaluation

To evaluate the anti-inflammatory effect of the composition of the present invention, NO activity was measured.

Specifically, RAW264.7 cells were aliquoted in a 96-well microplate ^{at a concentration of 1*10 3} cells/well, and then, 10% (v/v) fetal bovine serum (FBS), 100 units/ml penicillin, 100 μg Incubated for 24 hours in DMEM medium containing /ml streptomycin. Then, 0.01 μg/ml of LPS, Examples, and Comparative Example compositions were treated at a concentration of 25 μg/μl in each well and incubated for 24 hours. After transferring 50 μl of the supernatant to a new 96 well, nitric oxide (NO) ) 50 μl of the measurement reagent (Griess Reagent) was treated, reacted for 15 minutes, absorbance was measured at 540 nm, and NO activity (%) was calculated and shown in Table 3 below.

NO activity (%) Purified water 20.56 LPS 120.38 Example 1 38.23 Example 2 47.56 Comparative Example 1 90.32 Comparative Example 2 70.67 Comparative Example 3 88.95

As a result, it was confirmed that Examples 1 and 2 were significantly superior in the effect of inhibiting inflammation induced by LPS compared to Comparative Examples 1 to 3.

Test Example 4: Efficacy evaluation of female diseases

It was confirmed whether the composition of the present invention could be used for external use to improve vaginitis or groin skin health.

Specifically, the compositions of Examples 1 and 2 and Comparative Example 2 were applied to the groin area by dividing into groups of 5 for 30 women in their 20s to 40s, or after 10 to 20 drops per 1 liter of water were dropped. It was applied once a day in a left-handed manner, and a questionnaire survey was conducted on the feeling of use after 2 weeks. Each item on the feeling of use was evaluated as “Not at all (1), Not at all (2), Average (3), Yes (4), Very much (5)”, and the average is given in Table 4 below. indicated.

question direct application left Example 1 Example 2 Comparative Example 2 Example 1 Example 2 Comparative Example 2 Smell is improved 4.6 4 3.4 4.6 3.8 3.2 improved skin texture 4 3.2 2.6 4.4 4 2.8 Dryness symptoms improved 4.2 3.6 2.8 3.8 3.4 2.8 Symptoms such as skin tightness, itching, inflammation, and pain are improved 4 3.8 3 4 3.8 3.2

Claims (8)

6 parts by weight of a mixture of argan oil, jojoba oil, and evening primrose oil; and 4 parts by weight of a mixture of copaiba essential oil and lavender essential oil as an active ingredient, in a cosmetic composition for feminine hygiene care,
The argan oil, jojoba oil, and evening primrose seed oil are mixed in 1:1:1 parts by weight, and copaiba essential oil, and lavender essential oil are mixed in 1:1 parts by weight, a cosmetic composition for feminine hygiene care. The method according to claim 1, tea tree, clary sage (Frankincense), myr (Commiphora Myrrha), neroli (Neroli), rose, geranium (Geranium), Ylang Ylang (Ylang Ylang) as an additional ingredient, A cosmetic composition for feminine hygiene management, further comprising an extract of jasmine, jojoba, acacia flower, chamomile, vetiver, or a combination thereof. delete delete The method according to claim 1, formulated with one selected from the group consisting of oil, softening lotion, astringent lotion, nourishing lotion, lotion, nourishing cream, massage cream, essence, cleansing cream, cleansing foam, cleansing water, pack, spray, and powder. A cosmetic composition for feminine hygiene management. The method according to claim 1, The cosmetic composition for feminine hygiene management is a composition for improving or preventing vaginitis or groin inflammation, the cosmetic composition for feminine hygiene management. delete The cosmetic composition for feminine hygiene management according to claim 1, wherein the cosmetic composition for feminine hygiene management is used as an external application or for a training session.