KR20120003236A - Cosmetic composition containing broussonetia extract for improving acne - Google Patents

Abstract

PURPOSE: A cosmetic composition for treating acne is provided to ensure anti-inflammation and antibacterial effect and to relieve acne. CONSTITUTION: A cosmetic composition for treating acne contains Broussonetia kazinoki Sieb extract as an active ingredient. The composition is used for antibacterial effect against Propionibacterium acnes. The composition suppresses adipogenesis. The extract is contained in 0.001-50 wt% based on total composition.

Description

Cosmetic composition for improving acne containing a mulberry extract {Cosmetic composition containing Broussonetia extract for improving acne}

The present invention relates to a cosmetic composition for improving acne, and more particularly to a cosmetic composition that provides excellent inflammation relief and antimicrobial effect by containing a mulberry extract and is effective in treating or alleviating symptoms of acne.

Acne is an inflammatory disease of the fur branch, hair follicles and surrounding tissues caused by hormone action, excessive sebum secretion, bacterial infections, and the like.

The causes of acne can be divided into several ways. The first cause is excessive secretion of sebum. Sebum is a substance secreted by the sebaceous glands, and in normal amounts it acts to prevent harmful substances outside the skin from penetrating keratinocytes. However, under puberty, before and after menstruation, early pregnancy, contraceptives, and stress, the human body shows a different hormonal balance than normal. This altered hormonal balance stimulates the sebaceous glands, which leads to an oversecretion of sebum. There are also genetically high levels of sebum secretion and may be increased by changes in the outside temperature or by increased skin temperature due to poor eating habits.

The second cause is infection of the pores. Excessively secreted sebum contains a lot of fatty acids to increase the pH of the skin surface, dissolving the lipids of the skin lowers the defense of the skin itself. On the surface of the skin with high pH, acne-causing bacteria become easy to multiply, and the multiplying acne bacteria cause purulent inflammation through weakened skin.

In general, acne can be divided into four stages according to symptoms. The first stage is scleroderma acne, in which excessive secretion of sebum accumulates in the pores around the sebaceous glands, forming a scalp. Stage 1 spontaneous acne is an early stage of acne lesions, but cosmetically not good, causing skin concerns. Good care at this stage can also inhibit the development of inflammation. Stage 2 Papular Acne The acne germs multiply and cause inflammatory reactions that cause red acne, which is called papules and can cause scars if not properly managed. In the third stage purulent acne, the inflammatory reaction of red acne becomes more severe, and pus inside is formed. This is called pustules. In this stage, scars may remain even after treatment, so professional treatment is required. The final stage 4 nodular acne is a stage in which the pus breaks out of the skin and causes more inflammation around the acne lesion. Can be prolonged.

Treatment of acne includes topical formulations and oral medications, and surgical treatments such as laser and dermabrasion that have recently been performed.

Topical application therapy uses benzoyl peroxide for antibacterial effects such as azelaic acid and salicylic acid for the purpose of antibacterial action against propionibacterium acnes, which is known as the cause of acne. peroxide) is used as external ointment type. However, azelaic acid is difficult to formulate and salicylic acid is classified as a preservative in Korea, there is a problem that can not contain more than 0.5% in the formulation. In the case of benzoyl peroxide, there is a problem that it cannot be used as a cosmetic raw material in Korea.

Oral drug therapy uses retinoids and corticosteroids to inhibit sebaceous gland function and inhibit hair follicle wall destruction. These preparations have side effects such as skin rash, dry skin, and allergies may occur depending on the individual.

The problem of already developed, advanced acne and other skin diseases can be said that the external preparations used directly on the skin are more effective considering various aspects such as the speed and economical efficiency (see Korean Patent Publication No. 2004-0056081).

There are not many substances that can see these medical effects at the cosmetic stage. However, it can be used for a long time and has less damage to normal skin than a therapeutic drug. Therefore, many plant extracts and synthetic materials are being developed as a composition for improving acne skin, but there are not many products that have a therapeutic effect such as skin exfoliation after use and high skin side effects, which are safe for the skin and show the effect desired by consumers.

Accordingly, the present inventors have completed the present invention by confirming that the mulberry extract can alleviate the inflammatory symptoms as well as the antibacterial effect against the acne-causing bacteria, while researching to find a substance exhibiting excellent skin moisturizing effect among natural extracts.

Therefore, an object of the present invention is to provide a cosmetic composition for improving acne having an effect of improving the symptoms of acne by providing an anti-inflammatory, antimicrobial effect by containing the extract of the mulberry as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition for improving acne containing a mulberry extract as an active ingredient.

The cosmetic composition containing the extract of the mulberry of the present invention primarily exhibits its effect by rapidly inhibiting the growth and growth of microorganisms involved in skin acne development and skin inflammation, and secondly, excess sebum, which is one of the causes of acne, Improves acne skin by suppressing secretion and eliminating vaginal acne. It also has an anti-inflammatory effect that improves acne skin.

The cosmetic composition of the present invention contains a mulberry extract as an active ingredient.

The mulberry used in the present invention includes the mulberry (Broussonetia kazinoki Sieb), the beech (Broussonetia papyrifera Vent), the cedar (Broussonetia kazinoki var. Humilis), etc., a deciduous broad-leaved shrub most of Korea (mainly distributed in the south It is distributed in China, Taiwan, Japan, etc., and it spreads geographically on both sides of the foothills and field banks. Its bast fiber has been used as a raw material for papermaking, and it has various medicinal effects, which is effective in tonic, nominal effect, tone, species, ripening, diuresis, removes wind, and clears blood. It is known to be effective in reducing vision.

The extract of the mulberry used in the present invention is not only a leaching liquid obtained by leaching and transferring it from the mulberry, but also a concentrate obtained by partially or completely condensing the leaching liquid, or a stagnation, whole, regular, flow extract and dried mulberry prepared by drying the above concentrate again. It contains all the chemicals in the plant, as well as the main effect of the plant itself. In the present invention, extracts from all parts of the mulberry, such as stems, roots, leaves, flowers, fruits, etc. can be used, and are not limited to the extract of any particular part.

It is possible to use a well-known method as a manufacturing method of the mulberry extract used in the present invention. For example, the dried mulberry is dried by any method such as natural drying or forced drying and finely chopped, and then polar solvent such as water, ethanol, butanol, acetone, etc .; Nonpolar solvents such as ether, hexane, benzene, chloroform and ethyl acetate; Mixed solvents of the nonpolar and polar solvents; Using a solvent such as alkaline water, or vegetable oil such as soybean oil or sesame oil, it is extracted by any method such as cold sedimentation, percolation, and warm needle to obtain a leachate containing the active ingredient. The leaching treatment is about 12 to 96 hours for cold acupuncture and percolation, and depending on the type and temperature of the solvent used for warming, but preferably about 0.5 to 24 hours at a temperature close to the reflux temperature of the solvent. It is good. In particular, it is preferable to use a tincture, a liquid extract or an extract that has leached into the hydrous alcohol.

The cosmetic composition according to the present invention may contain 0.0001 to 50% by weight of the mulberry extract based on the total weight of the composition. If the amount of the extract of the mulberry extract less than 0.0001% by weight can not obtain a significant improvement in acne symptoms with the antibacterial, anti-inflammatory effect by the extract, if it exceeds 50% by weight is due to skin safety or formulation problems.

The cosmetic composition for improving acne according to the present invention is excellent in antibacterial effect, in particular, the antibacterial effect against acne causing bacteria, and also provides an anti-inflammatory effect.

Cosmetic compositions according to the invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) and non-obtained by dispersing an oil phase in solution, gels, solids, pasty anhydrous products, aqueous phases. It may be provided in the form of an ionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or concealed stick. It may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions may be prepared according to conventional methods in the art.

In addition, the cosmetic composition according to the present invention is a fatty substance, an organic solvent, a dissolving agent, a thickening agent, a gelling agent, a softener, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic type or a non- With ionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients commonly used in cosmetics. It may contain adjuvants commonly used in the same cosmetic or dermatology field. Such adjuvants are introduced in amounts generally used in the cosmetic or dermatological arts.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to test examples and examples. However, these test examples and examples are provided only for the purpose of illustration in order to help the understanding of the present invention, but the scope and scope of the present invention is not limited by the following examples.

Example 1 Preparation of Methanol Extract

After adding 1kg of dried mulberry starch to 10L purified water and heated until boiling, and further heated for 10 minutes, and then washed with water to remove, 10L purified water was added again and washed again. The residue was air-dried and added to 20L of 70% ethanol, and then connected to a reflux apparatus and then heated to reflux for 24 hours. After removing the solids using an 80 mesh sieve, the remaining filtrate was filtered again, and then the solvent in the filtrate was distilled off using a vacuum concentrator to obtain about 50 g of green solids.

[Formulation Example 1 and Comparative Formulation Examples 1 and 2]

Formulation Example 1 and Comparative Formulation Examples 1 and 2 were prepared according to the ingredients and the contents (% by weight) shown in Table 1 below. Specifically, Formulation Example 1 is a combination of the extract of the mulberry of Example 1, Comparative Formulation Example 1 does not contain any active ingredients for improving acne skin, Comparative Formulation Example 2 is based on the antimicrobial activity As a standard, it contains erythromycin, which is widely used as an acne treatment.

The preparation method of Formulation Example 1 and Comparative Formulation Examples 1 and 2 is as follows. The components of phase A in Table 1 were completely dissolved, and the components of phase B were completely dissolved in a separate dissolution tank, followed by mixing solubilization by adding phase B to phase A. The components of phase C were added thereto according to the blending ratios described in Table 1 to homogenize the mixture and then filtered to prepare the compositions.

division Formulation Example 1 Comparative Formulation Example 1 Comparative Formulation Example 2 A Deionized Water To 100 To 100 To 100 EDTA-2Na 0.02 0.02 0.02 glycerin 5.0 5.0 5.0 B ethanol PEG-60 hydrogenated castor oil 0.4 0.4 0.4 Perfume 0.04 0.04 0.04 C Example 1 5.0 - - Erythrombomycin - - 5.0

Test Example 1 Antibacterial Activity Test against Acne Bacteria

Antibacterial activity was tested against propionibacterium acnes <ATCC 6919: Medium-BHI broth>, an acne-causing strain, with the respective cosmetic compositions prepared in the formulations of Formulation Example 1 and Comparative Formulation Examples 1-2. .

The antibacterial test method for acne bacteria was as follows.

(1) Test bacteria preparation

Propionibacterium acnes was used as a culture broth inoculated in BHI broth and anaerobic culture.

(2) dilution solution preparation

0.15 ml of the test bacteria was added to 15 ml of BHI broth (pH 6.8) or LB broth (pH 4.5) and mixed well as a dilution solution.

(3) sample preparation

The cosmetic composition stock solutions prepared from Formulation Example 1 and Comparative Formulation Examples 1 and 2 were used as samples.

(4) antimicrobial activity test

1) Insert the sample to the starting concentration in the first well of 96-well cell culture tube (96 well plate) and add 200 μl of total amount into the dilution solution.

2) Mix the mixed solution in the first row well, take 100μl into the second row, mix well, and then add 100μl to the third row and perform double dilution.

3) After incubation for 24 hours and 48 hours at 32 ℃, determine the growth of bacteria to the extent of suspension and determine the minimum concentration without growth of bacteria as MIC (Minimum Inhibitory Concentration) value. If the mixed solution is opaque and it is difficult to determine the growth of bacteria, check through a microscope.

The antimicrobial activity test results for acne bacteria are shown in Table 2 below. MIC is expressed in terms of the concentration of the active ingredient contained in the formulation.

Item pH Propionibacterium Acnes Formulation Example 1 5.7 <87 ppm Comparative Formulation Example 1 5.7 Maximum concentration (no antibacterial activity) Comparative Formulation Example 2 5.7 <100 ppm

The smaller the ppm concentration in MIC, the more effective the antimicrobial activity against acne bacteria. In the case of Formulation Example 1, the concentration of ppm was lower than that of Comparative Formulation Example 2 using the well-known acne treatment erythrombomycin. It can be confirmed that it has excellent antimicrobial activity.

Experimental Example 2 Lipogenesis Inhibition Test

3T3-L1 cells, a mouse fibroblast cell line, were loaded with DMEM (Dulbecos modified eagles medium, GIBCO BRL, Life Technologes) medium containing 10% fetal bovine serum (FBS). The well culture plate was attached at 1 × 10 ⁵ cells / well. After 2 days, it was again exchanged with fresh DMEM (containing 10% FBS) medium and incubated for 2 days. The incubated cells were then induced to differentiate with DMEM (containing 10% FBS) containing 1 μg / ml insulin, 0.5 mM IBMX and 0.25 μM dexamethasone, and again after 2 days. Incubated for 5 days by exchange with DMEM containing insulin. After 5 days, the cells were exchanged with normal medium (DMEM, containing 10% FBS) and cultured while observing until the cells changed to fat cells.

Sudan III staining (S4136, sigma-aldrich) was performed using the differentiated 3T3-L1 adipocytes to evaluate the inhibitory effect of adipocytes on adipocyte accumulation. Adipose cells were fixed at room temperature with 4% paraformaldehyde (pH 7.2) in phosphate buffer, washed with PBS (phsphate buffered saline), stained with Sudan III, and photographed for visual comparison. As a control group, only the medium without the test substance or the comparative substance was used, and 50 μM of caffeine was treated with another control group. The degree of fat accumulation inhibition was graded by dividing the degree of staining into +++, ++, +,-. The results are shown in Table 3.

sample % Inhibition Control group +++ Comparison + Example 1 +

As shown in Table 3, it can be seen that the mulberry extract used in the present invention has a lipid synthesis inhibitory effect. Therefore, sebum is reduced by inhibiting lipid synthesis, thereby suppressing the occurrence of acne.

[Test Example 3] Test for acne improvement and sebum secretion and irritation

Twelve people with acne were allowed to use the cosmetic composition prepared from Formulation Example 1 and Comparative Formulation Examples 1 and 2 for one month. The acne improvement scale ranged from 1 to 5 points, with 1 being ‘no’, 3 being ‘normal’ and 5 being ‘very yes’. The experimental results are expressed in the average score of 12 people in Table 4 below.

Acne disappearance was based on the number of days the extinction was read, acne recurrence was based on the results after one month. Sebum secretion is reduced from 1 to 5 points, with 1 being ‘no’, 3 being ‘normal’ and 5 being ‘very yes’. The experimental results are expressed in the average score of 12 people in Table 4 below. The presence or absence of skin irritation was determined as (number of irritants) / (total number of test subjects).

Inflammatory Acne Improvement Cotton acne extinction Acne recurrence Reduce sebum secretion Irritation Formulation Example 1 3.2 4 days radish 4.5 0/12 Comparative Formulation Example 1 2.1 13th U 2.0 0/12 Comparative Formulation Example 2 4.2 2 days radish 4.1 9/12

As shown in Table 4, Formulation Example 1 did not recur acne compared to Comparative Formulation Example 1, it can be seen that there is an excellent effect on the overall acne improvement. On the other hand, Comparative Formulation Example 2 containing the antimicrobial activity standard, but shows a similar efficacy as Formulation Example 1, there is a fear of skin irritation in the long-term use is strong irritation in use.

Test Example 4 Inflammation Improvement

The anti-inflammatory effect was evaluated by the inhibitory effect of the production of prostaglandins. The effect was measured in macrophages using the extract of the mulberry of Example 1. First, aspirin was added to macrophages taken from the abdominal cavity of mice to a final concentration of 500 M, thereby irreversibly inhibiting cyclooxygenase (COX) activity remaining in the cells. Then, 100 μl of the suspension was added to each well of the 96 well cell culture tube and incubated for 2 hours in an incubator at 37 ° C. with 5% CO ₂ to attach macrophages to the container surface. The attached macrophages were then washed three times with PBS and used for the effect test of the extract. The cultured macrophages 5x10 ⁴ cells / ml by adding RPMI medium containing 1% (w / v) of LPS incubated for 12 hours to induce the production of prostaglandin and 100μl of the extract to the free prostaglandin enzyme Quantification using an immunoassay (ELISA).

In this case, the production inhibitory activity of the prostaglandin of the extract was set to 100% the difference between the prostaglandin produced in the LPS-treated and untreated groups, respectively, and treated with LPS and the sample to reduce the percentage of prostaglandin The results were compared and determined, and the results (the effect of inhibiting production of prostaglandins) are shown in Table 5 below.

Blank 100% Control group (Aspirin treated group) 25.0% Example 1 26.1%

As shown in Table 5, the experimental results, even when treated with Example 1 as a control group treated with aspirin it can be seen that the effect of inhibiting the production of prostaglandin is very high.

It can be seen that the mulberry extract of the present invention can provide an excellent inflammation improving effect.

Claims (6)

Cosmetic composition for acne improvement containing the extract of the mulberry as an active ingredient. According to claim 1, wherein the composition is a cosmetic composition, characterized in that the antibacterial. According to claim 2, wherein the composition is a cosmetic composition, characterized in that the antibacterial against acne cause bacteria Propionibacterium acnes. According to claim 1, wherein the composition is a cosmetic composition, characterized in that for anti-inflammatory. The cosmetic composition according to claim 1, wherein the composition inhibits lipid formation. The cosmetic composition according to any one of claims 1 to 5, wherein the mulberry extract is contained in an amount of 0.001 to 50% by weight based on the total weight of the composition.