KR102279878B1 - Cosmetic composition for improving acne - Google Patents

Abstract

The present invention relates to a cosmetic composition, and specifically, Dipotassium Glycyrrhizate 0.01 to 0.07 wt%, Camellia Oil 0.01 to 0.4 wt%, Epilobium Fleischeri Extract 0.05 to 0.25% by weight, Witch Hazel Extract 0.1 to 2% by weight, Willow Bark Extract 0.1 to 0.7% by weight, and Camomile Extract 0.1 to 0.4% by weight. The composition of the present invention is an invention that can contribute to the cosmetic industry because it has excellent skin soothing and acne improvement effects, and excellent moisturizing and elasticity improvement effects.

Description

Cosmetic composition for improving acne {COSMETIC COMPOSITION FOR IMPROVING ACNE}

The present invention relates to a cosmetic composition.

Acne is an inflammation of many hair follicles on the face of adolescent males and females, especially the cheeks and forehead. Sometimes it occurs on the chest or back, and sometimes it occurs around the age of 40. At first, the hair follicles are filled with cortex and dead skin cells, forming a yellowish-white mass, and sometimes the apex may be discolored black. When you squeeze it, a cheese-like thing comes out. Then, it becomes inflamed and red bumps around it. If it is infected with bacteria, it becomes purulent and turns yellow. A better spot leaves a crater-shaped trace. Usually these four types are mixed. The cause is often caused by bacterial infection in the sebum secreted excessively by innate disposition and the action of sex hormones, especially male hormones. In women over the age of 22, it often worsens just before menstruation. Although the cause of acne is not yet clearly known, it can be generally summarized as increased sebum secretion, keratinization of the skin and peroxidation of lipid components, proliferation of acne-causing bacteria, and induction and exacerbation of inflammation. One of the important characteristics related to the onset of acne is an increase in sebum secretion, which is known to be closely related to androgens, a male hormone. As male hormones are secreted above the normal level, the function of the sebaceous glands is enhanced, and the excess sebum produced in this way is stagnated in the hair follicles due to keratinization of the hair follicle wall, and comedones are generated. The sebum accumulated in the hair follicle, which cannot be discharged to the outside, blocks the pores and prevents the inflow of external air, so that the inside of the hair follicle becomes an environment in which the anaerobic bacteria, Propionibacterium acnes, can smoothly proliferate. Propionibacterium acnes secretes lipolytic enzymes and chemical factors to make free fatty acids, triggers an immune response of leukocytes, destroys the hair follicle wall, and the contents leak into the dermis, resulting in an inflammatory reaction. . Therefore, a general treatment method for acne can be summarized in four cases: suppression of excessive sebum production, suppression of keratinization of hair follicle wall, suppression of proliferation of propionibacterium acnes, and minimization or suppression of inflammatory response. .

Various microorganisms exist on the human skin. These microorganisms cause various skin diseases. Staphylococcus aureus, which causes suppuration in the skin, and Staphylococcus epidermis, Propionibacterium acnes, etc. It decomposes to generate odor, and the decomposition products irritate the skin and cause inflammation. In particular, Propionibacterium acnes produces free fatty acids by decomposing sebum using lipase, which is a fatty acid decomposing enzyme. These free fatty acids not only irritate the skin, but also cause inflammatory lesions such as papules, pustules, and nodules, which are red rashes seen in acne.

Although various drugs have been developed and used in the treatment area of acne, the reality is that the selection of cosmetics for proper skin care has not yet been studied. In the cosmetic field, it is a concept to heal acne by removing hair follicle stenosis mainly through skin exfoliation with soap, medicinal soap, abrasive cleanser, etc. In addition, the alcohol component of Astrgent has an effect on acne to some extent as well as the skin exfoliation and sterilization effect of acne bacteria. There are also cosmetics containing resorcinol, sulfur, salicylic acid, benzoyl peroxide, and the like. However, these raw materials have a number of side effects and limitations, such as limiting the concentration of use due to skin irritation.

In addition, antibiotics such as erythromycine for the removal of acne bacteria, anti-inflamation raw materials for inflammation treatment, and hormone preparations for sebum control are used, but these are mainly raw materials in the pharmaceutical field.

The products so far have been developed focusing on only one of the various mechanisms of occurrence of acne described above, so there is a problem that the healing effect is insignificant and recurrence is frequent. Also, there is no preventive concept, and there is no product with a healing concept in the cosmetic field.

Korean Patent No. 1749690

An object of the present invention is to provide a cosmetic composition having skin soothing and acne improving effects.

1. Dipotassium Glycyrrhizate 0.01 to 0.07 wt%, Camellia Oil 0.01 to 0.4 wt%, Epilobium Fleischeri Extract 0.05 to 0.25 wt%, witch hazel extract ( Witch Hazel Extract) 0.1 to 2% by weight, willow bark extract (Willow Bark Extract) 0.1 to 0.7% by weight, and chamomile extract (Camomile Extract) comprising 0.1 to 0.4% by weight, a cosmetic composition.

2. The above 1, vitamin B6 / HCl 0.02 to 0.1% by weight, niacin amide (Niacin Amide) 0.1 to 1% by weight, methyl sulfonyl methane (Methyl Sulfonyl Methane, MSM) 0.1 to 0.5% by weight, salicylic acid (Salicylic Acid) ) 0.04 to 0.2% by weight, Aloe Leaf Extract 0.1 to 2% by weight, and glycolic acid (Glycolic Acid) 0.04 to 0.2% by weight, the cosmetic composition further comprising.

3. In the above 2, 0.1 to 0.4% by weight of glucose and fructose mixture, 1 to 7% by weight of extract mixture of omija, ginger, yellow lily and green tea, and 0.1 to 2% by weight of extract mixture of Propolis and Machihyeon (Portulaca Oleracea) Further comprising, a cosmetic composition.

4. The cosmetic composition of 1 above, wherein the composition is for improving acne.

The composition of the present invention has excellent skin soothing and acne improvement effects, and excellent moisturizing and elasticity improvement effects.

Hereinafter, the present invention will be described in detail.

The present invention is Dipotassium Glycyrrhizate 0.01 to 0.07% by weight, Camellia Oil 0.01 to 0.4% by weight, Epilobium Fleischeri Extract 0.05 to 0.25% by weight, witch hazel extract (Witch Hazel Extract) 0.1 to 2% by weight, Willow Bark Extract (Willow Bark Extract) 0.1 to 0.7% by weight and chamomile extract (Camomile Extract) comprising 0.1 to 0.4% by weight, relates to a cosmetic composition.

The cosmetic composition of the present invention is excellent in moisturizing and elasticity improvement effects as well as skin soothing and acne improvement effects.

The dipotassium glycyrrhizate is for imparting a conditioning effect to the skin and alleviating skin irritation, and the content of the dipotassium glycyrrhizate is 0.01 to 0.07% by weight, preferably 0.03 to 0.05% by weight. %, more preferably 0.035 to 0.045 wt%. However, the present invention is not limited thereto.

The camellia oil is to increase the flexibility of the composition and improve trouble, and the content of the camellia oil is 0.01 to 0.4% by weight, preferably 0.1 to 0.3% by weight, more preferably 0.15 to 0.25% by weight. %, but is not limited thereto.

The Epilobium plaischeri extract has an anti-inflammatory effect and is intended to increase metabolic function, and the content of the Epilobium plaischeri extract is 0.05 to 0.25% by weight, preferably 0.1 to 0.2% by weight. %, more preferably 0.13 to 0.17 wt%, but is not limited thereto.

The Epilobium placeri extract may be extracted from the leaves, roots and stems of Epilobium placeri.

As used herein, the term "extract" refers to an extraction target regardless of the extraction method, for example, ethanol, methanol, acetone, ethyl acetate, saturated n-butanol, chloroform, methylene chloride, water, or a solvent containing a mixture thereof. It is understood to include extracts obtained by extraction, and extracts fractionated with the solvents enumerated above in the extracts. As long as the extraction is extracted through the step of immersing the extraction target in the extraction solvent, it should be understood that any method such as cooling, reflux, heating, ultrasonication, etc. may be applied. In one embodiment, the extract is obtained by extracting the extraction target with water, ethanol, or a mixed solvent thereof, and may mean including a concentrated liquid extract or a solid extract from which the extraction solvent is removed.

According to an embodiment of the present invention, the amount of the solvent relative to the content to be extracted of the "extract" may be 5 to 35 times, preferably 10 to 30 times, more preferably 15 to 25 times, and the concentration of the solvent is 50 to 90% (v/v), preferably 60 to 80% (v/v), more preferably 65 to 75% (v/v), but is not limited thereto.

In addition, according to an embodiment of the present invention, the extraction method of the "extract" is 1 to 5 hours, preferably 2 to 4 hours, more preferably 2 hours 30 minutes to 3 hours 30 minutes in a hot extractor. It may be obtained by extraction and freeze-drying, but is not limited thereto.

The temperature of the hot water may be, for example, 70 to 130 ℃, preferably 80 to 120 ℃, more preferably 90 to 110 ℃, but is not limited thereto.

The witch hazel extract is a substance that causes an astringent action of the skin and controls sebum production, and may be extracted from leaves, roots and stems of witch hazel. The content of the witch hazel extract (Witch Hazel Extract) may be 0.1 to 2% by weight, preferably 0.5 to 1.5% by weight, more preferably 0.8 to 1.2% by weight, but is not limited thereto.

The willow bark extract activates cells, can heal inflammation, and has an antibiotic effect, and the content of the willow bark extract is 0.1 to 0.7% by weight, preferably 0.2 to 0.6% by weight. %, more preferably 0.3 to 0.5 wt%, but is not limited thereto.

The chamomile extract has a skin soothing effect and anti-inflammatory effect, and may be extracted from leaves, stems, roots, etc. of chamomile. The content of the chamomile extract (Camomile Extract) may be 0.1 to 0.4% by weight, preferably 0.2 to 0.3% by weight, more preferably 0.23 to 0.27% by weight, but is not limited thereto.

Each component included in the composition is excellent in moisturizing and elasticity improvement effects within each content range, and furthermore, skin soothing and acne improvement effects are excellent.

According to an embodiment of the present invention, the composition of the present invention is vitamin B6 / HCl 0.02 to 0.1% by weight, niacin amide (Niacin Amide) 0.1 to 1% by weight, methyl sulfonyl methane (Methyl Sulfonyl Methane, MSM) 0.1 to 0.5 % by weight, Salicylic Acid 0.04 to 0.2% by weight, Aloe Leaf Extract 0.1 to 2% by weight, and Glycolic Acid 0.04 to 0.2% by weight.

The vitamin B6/HCl means a hydrochloride salt of vitamin B6, has an anti-inflammatory effect and a trouble improving effect, and the content of the vitamin B6/HCl is 0.02 to 0.1% by weight, preferably 0.04 to 0.08% by weight, more preferably may be 0.05 to 0.07% by weight, but is not limited thereto.

The niacin amide is for a whitening effect and an inflammatory healing effect, and the content of the niacin amide may be 0.1 to 1% by weight, preferably 0.3 to 0.7% by weight, more preferably 0.4 to 0.6% by weight. However, the present invention is not limited thereto.

The methyl sulfonyl methane is for natural sulfur-toxin removal, and the content of the methyl sulfonyl methane (MSM) is 0.1 to 0.5% by weight, preferably 0.2 to 0.4% by weight, more preferably 0.25 to 0.35% by weight, but is not limited thereto.

The salicylic acid is for keratin improvement and antibacterial effect, and the content of the salicylic acid may be 0.04 to 0.2% by weight, preferably 0.07 to 0.17% by weight, more preferably 0.1 to 0.14% by weight. It is not limited.

The aloe leaf extract is a component for wound healing, moisturizing effect and erythema prevention, and the content of the aloe leaf extract is 0.1 to 2% by weight, preferably 0.5 to 1.5% by weight, more preferably 0.9 to 1.1% by weight, but is not limited thereto.

The glycolic acid is for keratin improvement, antibacterial effect and acidity control, and the content of glycolic acid is 0.04 to 0.2% by weight, preferably 0.07 to 0.17% by weight, more preferably 0.1 to 0.14% by weight. However, it is not limited thereto.

Each component further included in the composition has excellent moisturizing and elasticity improvement effects within each content range, and further has excellent skin soothing and acne improvement effects.

Further, according to one embodiment of the invention, the composition of the present invention is glucose and fructose mixture 0.1 to 0.4% by weight of Schisandra chinensis, ginger, goldthread, and green tea extract mixture is 1 to 7% by weight and propolis (Propolis) and Portulaca Oleracea (Portulaca Oleracea ) may further comprise 0.1 to 2% by weight of the extraction mixture.

The glucose and fructose mixture is for reducing skin irritation and controlling sebum production, the mixing ratio of the glucose and fructose is not particularly limited, and the content of the glucose and fructose mixture is 0.1 to 0.4% by weight, preferably 0.2 to 0.3% by weight, more preferably 0.23 to 0.27% by weight, but is not limited thereto.

The omija, ginger, yellow lily and green tea extract mixture is for natural moisturizing, antibacterial and anti-inflammatory effects, and the content ratio of omija, sage, yellow lily and green tea extract is not particularly limited, and the omija, ginger, yellow lily and green tea extract mixture is not particularly limited. The content of may be 1 to 7% by weight, preferably 3 to 5% by weight, more preferably 3.5 to 4.5% by weight, but is not limited thereto.

As the propolis and Portulaca Oleracea extraction mixture components for improving skin problems and irritation, increased moisture, the mixing ratio is not particularly limited, the propolis (Propolis) and Portulaca Oleracea (Portulaca Oleracea) content of the extraction mixture is from 0.1 to 2 % by weight, preferably 0.5 to 1.5% by weight, more preferably 0.9 to 1.1% by weight, but is not limited thereto.

Each component further included in the composition has excellent moisturizing and elasticity improvement effects within each content range, and further has excellent skin soothing and acne improvement effects.

In addition, according to an embodiment of the present invention, the composition of the present invention is for improving acne.

The composition according to an embodiment of the present invention specifically has antibacterial activity against Acne bacteria, for example, Propionibacterium acnes, can reduce sebum by inhibiting lipid synthesis, and can improve inflammation.

In addition, the composition according to an embodiment of the present invention may further include a solvent, a thickener, an anti-freezing agent, a humectant, a fragrance, a preservative, a surfactant, an antioxidant, a blood circulation promoter and an additive.

The solvent may be water, preferably distilled water, and the content of the solvent may be 65 to 95% by weight, preferably 70 to 90% by weight, more preferably 75 to 85% by weight, but limited thereto no.

The thickener is at least one of sodium polyacrylate or carbomer, and the content of sodium polyacrylate is 0.05 to 0.35% by weight, preferably 0.1 to 0.3% by weight, more preferably may be 0.15 to 0.25 wt%, and the content of the carbomer may be 0.1 to 0.7 wt%, preferably 0.2 to 0.6 wt%, more preferably 0.3 to 0.5 wt%, but limited thereto it is not The sodium polyacrylate and carbomer may also serve as a stabilizer of the composition according to an embodiment of the present invention.

The cryoprotectant may be glycerin, and the content of the glycerin may be 1 to 5% by weight, preferably 2 to 4% by weight, more preferably 2.5 to 3.5% by weight, but is not limited thereto. . The glycerin may also serve as a desiccant of the composition according to an embodiment of the present invention.

The moisturizing agent may be at least one selected from the group consisting of betaine, 1.3 Butylene Glycol, and hyaluronic acid, and the content of the betaine is 0.1 to 1.1% by weight, preferably 0.4 to 0.8% by weight, more preferably may be 0.5 to 0.7% by weight, and the content of 1.3 Butylene Glycol may be 0.5 to 2.5% by weight, preferably 1 to 2% by weight, more preferably 1.3 to 1.7% by weight, and the content of hyaluronic acid Silver may be 0.1 to 1% by weight, preferably 0.3 to 0.7% by weight, more preferably 0.4 to 0.6% by weight, but is not limited thereto.

The preservative is triclosan, butylene glycol (Butylene Glycol), capryl hydroxamic acid, 1,2-hexanediol (1,2-Hexanediol) and Indian frankincense extract (Boswellia Serrata) Resin Extract) may be at least one selected from the group consisting of, and the content of triclosan may be 0.1 to 3% by weight, preferably 0.5 to 2.5% by weight, more preferably 1 to 2% by weight, and the butyl Rene glycol (Butylene Glycol), capryl hydroxamic acid (Capryl Hydroxamic Acid), 1,2-hexanediol (1,2-Hexanediol) and Indian frankincense resin extract (Boswellia Serrata Resin Extract) selected from the group consisting of The at least one content may be 0.1 to 2% by weight, preferably 0.5 to 1.5% by weight, more preferably 0.8 to 1.2% by weight, but is not limited thereto.

The surfactant may be polyoxyethylene (60) hydrogenated castor oil, and the content of the polyoxyethylene (60) hydrogenated castor oil is 0.1 to 0.4% by weight, preferably 0.2 to 0.3% by weight, more preferably 0.23 to 0.27% by weight, but is not limited thereto.

The antioxidant may be vitamin E/acetate, and the content of the vitamin E/acetate may be 0.01 to 0.15% by weight, preferably 0.04 to 0.12% by weight, more preferably 0.06 to 0.1% by weight, but limited thereto. it's not going to be

The blood circulation promoting agent may be a hawthorn extract, and the content of the hawthorn extract may be 0.1 to 1% by weight, preferably 0.3 to 0.7% by weight, more preferably 0.4 to 0.6% by weight, but is limited thereto no. The hawthorn extract can relieve itching of the skin.

The additive may be at least one of cetearyl alcohol or L-arginine, and the content of cetearyl alcohol is 0.01 to 0.2% by weight, preferably 0.05 to 0.15% by weight, More preferably, it may be 0.08 to 0.12% by weight, and the content of L-arginine may be 0.26 to 0.7% by weight, preferably 0.38 to 0.58% by weight, more preferably 0.46 to 0.5% by weight, but limited thereto it's not going to be The cetearyl alcohol may increase the flexibility of the composition according to an embodiment of the present invention, and the L-arginine may be used to adjust the acidity of the composition according to an embodiment of the present invention.

In addition, the composition of the present invention may additionally include conventional adjuvants and carriers such as components commonly added to cosmetic compositions, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances.

The cosmetic composition may be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil , powder foundation, emulsion foundation, wax foundation and spray, etc., but are not limited thereto. More specifically, lotions such as flexible lotions or nourishing lotions, emulsions such as facial lotions and body lotions, creams such as nourishing creams, moisture creams, and eye creams, essences, cosmetic ointments, sprays, gels, packs, sunscreens, makeup It may be prepared in the form of a foundation, such as a base, liquid type, solid type, or spray type, or powder. In this case, the cosmetic composition comprises an emulsifier, soapy acid, solvent, colorant, preservative, antioxidant, antifoaming agent, antibacterial agent, anti-redeposition agent, enzyme, plant or mineral oil, fat, fluorescent substance, fungicide, hydrophobicity-inducing substance, moisturizer, It may contain excipients including fragrances, fragrance carriers, preservatives, proteins, silicones, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, waxes, and the like.

When the formulation of the cosmetic composition is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component can be used

When the formulation of the cosmetic composition is a powder or a spray, toss, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propellants such as propane/butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester; Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth may be used.

Hereinafter, examples will be given to describe the present invention in detail.

Preparation Example - Preparation of the cosmetic composition of the present invention

A first mixture was obtained by dispersing and injecting 0.6 kg of Cosmedia SP (Daebong LS, Korea) as sodium polyacrylate and 1.2 kg of Carbopol 940 as Carbomer in 233.52 kg of distilled water as a solvent in the dissolution tank.

Then, as Dipotassium Glycyrrhizate, DPG-2K (BioR&G, Korea) 0.12kg, vitamin B6/HCL (Lee Hyun Chemical, Korea) 0.18kg, glycerin 9kg, Betaine (BioR) Angie, Korea) 1.8kg, niacinamide (Lee Hyun Chemical, Korea) 1.5kg, and methyl sulfonyl methane (MSM) (BioRNG, Korea) 0.9kg were sequentially injected into the dissolution tank to prepare the second mixture. got it

Fragrance (Water Lily) 0.18kg, Triclosan (Lee Hyun Chemical, Korea) 0.45kg, cetearyl alcohol, Kalcol 6870 0.3kg, salicylic acid (BioR&G, Korea) 0.36kg, polyoxyethylene (60) hydrogenated As a castor oil (Polyoxyethylene (60) Hydrgenated Castor Oil), 0.75 kg of HCO-60, 0.24 kg of vitamin E/acetate (Lee Hyun Chemical, Korea), 0.6 kg of camellia oil (Bio R&G, Korea) and 4.5 kg of 1.3 Butylene Glycol were mixed with 60 A third mixture was obtained by mixing while heating and melting in a separate dissolution tank at °C. Thereafter, the third mixture was cooled to 40° C. and then poured into a dissolution tank and sufficiently stirred to obtain a fourth mixture.

1.5 kg of 1% Hyal Oligo as hyaluronic acid (Shinaviti, Korea), 0.6 kg of Pentavitin (Interlis, Korea) as a mixture of glucose and fructose (Saccharide Isomerate), Butylene Glycol, caprylic hydrooxamic acid (Capryl Hydroxamic Acid), 1,2-Hexanediol, and Bioneat WS (BioR&G, Korea) 3kg as a mixture of Boswellia Serrata Resin Extract, Epilobium placery extract ( Epilobium Fleischeri Extract) ALP-SEBUM (Interlis, Korea) 0.45 kg, Aloe Leaf Extract (Bioland, Korea) 3 kg, Na3 Hawthorn (Dami Chemical, Korea) 1.5 kg as hawthorn extract, Witch Witch Hazel Extract (Bioland, Korea) 3kg, Bio Protector (Dr. Lanka, Korea) 12kg, Willow Bark Extract (BioR&G, Korea) as a mixture of extracts of omija, ginger, yellow lily and green tea ) 1.2 kg, Propolis and Portulaca Oleracea extract mixture, Oriental Trois 2PK (BioRNG, Korea) 3kg, Glycolic Acid (BioRNG, Korea) 0.36kg and chamomile extract (Camomile) Extract) (Shin Abiti, Korea) 0.75 kg was sequentially injected into the dissolution tank, and stirred to obtain a fifth mixture.

In another separate dissolution tank, 1.44 kg of L-arginine (Lee Hyun Chemical, Korea) was dissolved in 12 kg of distilled water as a solvent to obtain a sixth mixture.

Then, by injecting the sixth mixture into the dissolution tank, defoaming and mixing for 30 minutes to obtain 300 kg of the composition of the present invention.

Examples and Comparative Examples

According to the preparation example, Examples 1 to 3 and Comparative Examples 1 to 6 were prepared with the components and contents shown in Table 1 below.

At this time, each extract was obtained by adding 70% ethanol 20 times the content of each material, hot water extraction at 100° C. in a heat extractor for 3 hours, and freeze-drying the extract.

Dipotassium glycyrrhizate
(Dipotassium Glycyrrhizate) camellia oil
(Camellia Oil) Epilobium placidori extract
( Epilobium Fleischeri Extract) witch hazel extract
(Witch Hazel Extract) willow bark extract
(Willow Bark Extract) chamomile extract
(Camomile Extract) Centella asiatica Urbain extract Example 1 0.04 wt% 0.2 wt% 0.15 wt% 1% by weight 0.4% by weight 0.25% by weight - Example 2 0.05% by weight 0.3% by weight 0.2 wt% 1.5% by weight 0.6 wt% 0.3% by weight - Example 3 0.03 wt% 0.1% by weight 0.1% by weight 0.5% by weight 0.2 wt% 0.2 wt% - Comparative Example 1 - 0.2 wt% 0.15 wt% 1% by weight 0.4% by weight 0.25% by weight 0.04 wt% Comparative Example 2 0.04 wt% - 0.15 wt% 1% by weight 0.4% by weight 0.25% by weight 0.2 wt% Comparative Example 3 0.04 wt% 0.2 wt% - 1% by weight 0.4% by weight 0.25% by weight 0.15 wt% Comparative Example 4 0.04 wt% 0.2 wt% 0.15 wt% - 0.4% by weight 0.25% by weight 1% by weight Comparative Example 5 0.04 wt% 0.2 wt% 0.15 wt% 1% by weight - 0.25% by weight 0.4% by weight Comparative Example 6 0.04 wt% 0.2 wt% 0.15 wt% 1% by weight 0.4% by weight - 0.25% by weight Sodium Polyacrylate 0.2 wt%
Carbomer 0.4 wt%
Glycerin 3 wt%
Betaine 0.6 wt%
1.3 Butylene Glycol 1.5 wt%
0.5% by weight of hyaluronic acid
Triclosan 1.5% by weight
Butylene Glycol, Capryl Hydroxamic Acid, 1,2-Hexanediol, and Boswellia Serrata Resin Extract 1 wt%
Polyoxyethylene (60) Hydrogenated Castor Oil 0.25 wt %
Vitamin E/Acetate 0.08 wt%
0.5 wt% hawthorn extract
Cetearyl alcohol 0.1 wt%
L-Arginine 0.48 wt%
distilled water

In addition, Example 4 included the following components and contents instead of 1.2 wt% of distilled water of Example 1, and was prepared according to Preparation Example 1: Vitamin B6/HCl 0.06 wt%, Niacin Amide 0.5 wt%, methyl 0.3% by weight of Methyl Sulfonyl Methane (MSM), 0.12% by weight of Salicylic Acid, 0.1% by weight of Aloe Leaf Extract and 0.12% by weight of Glycolic Acid.

Comparative Example 7 contains 1.2 wt% of Centella asiatica Urbain extract instead of 1.2 wt% of distilled water of Example 1, and was prepared according to Preparation Example 1.

In addition, Example 5 included the following ingredients and contents instead of 5.25 wt% of distilled water of Example 4, and was prepared according to Preparation Example 1: 0.25 wt% of a mixture of glucose and fructose, 4 wt.% of a mixture of extracts of Schisandra, ginger, yellow lily and green tea % and 1% by weight of Propolis and Portulaca Oleracea extract mixture.

Comparative Example 8 contains 5.25 wt% of Centella asiatica Urbain extract instead of 5.25 wt% of distilled water of Example 4, and was prepared according to Preparation Example 1.

Experimental example

1. Antibacterial activity test against acne bacteria

The cosmetic compositions of Examples 1 to 5 and Comparative Examples 1 to 8, and with erythrombomycin, a known acne treatment agent, as a control, antibacterial activity against Propionibacterium acnes (ATCC 6919: medium-BHI broth), which is an acne-causing strain was tested. The antibacterial activity test method for acne bacteria was as follows.

(1) Preparation of test microbial solution

Propionibacterium acnes was inoculated into BHI broth and anaerobic culture was used.

(2) Preparation of dilution solution

0.15ml of the test bacterial solution was added to 15ml of BHI broth (pH 6.8) or LB broth (pH 4.5) and well mixed was used as a dilution solution.

(3) Sample preparation

The cosmetic compositions of Examples 1 to 5 and Comparative Examples 1 to 8, and erythrombomycin stock solutions were used as samples.

(4) Antibacterial activity test

1) In a 96-well cell culture tube (96 well plate), put a sample in row 1 to match the starting concentration, and add 200 μl of the total amount as a dilution solution.

2) After mixing the mixture in row 1 well, take 100 μl, put it in row 2, mix well, then take 100 μl again and put it in row 3 for double dilution.

3) After stationary culture at 32°C for 24 hours and 48 hours, the proliferation of bacteria is determined to the extent of suspension, and the minimum concentration without proliferation of bacteria is determined as the MIC (Minimum Inhibitory Concentration) value. If the mixed solution is opaque and it is difficult to determine whether or not the growth of bacteria is present, check through a microscope.

The results of the antibacterial activity test against Acne bacteria are shown in Table 2 below. MIC was expressed in terms of the concentration of the active ingredient contained in the formulation.

division Prypionibacterium acnes (MIC) Example 1 87 ppm Example 2 85 ppm Example 3 89 ppm Example 4 75 ppm Example 5 61 ppm Comparative Example 1 132 ppm Comparative Example 2 141 ppm Comparative Example 3 130 ppm Comparative Example 4 124 ppm Comparative Example 5 138 ppm Comparative Example 6 126 ppm Comparative Example 7 81 ppm Comparative Example 8 72 ppm erythrombomycin 100 ppm

The smaller the ppm concentration in the MIC, the more effective the substance against the antibacterial activity against acne bacteria. In the case of Examples 1 to 5, the ppm concentration was smaller than that of erythrombomycin, a known acne treatment agent, and Comparative Examples 1 to 6, Example 4 was smaller than Comparative Examples 1 to 7, and Example 5 was smaller than Comparative Examples 1 to 8, confirming that the composition of the present invention had excellent antibacterial activity against the test bacteria.

2. Lipogenesis inhibition test

3T3-L1 cells, a mouse fibroblast cell line, were treated with DMEM (Dulbecos modified eagles medium, GIBCO BRL, Life Technologes) medium containing 10% fetal bovine serum (FBS). 1×10 ⁵ cells/well were adhered to a well culture plate. After 2 days, the medium was replaced with fresh DMEM (containing 10% FBS) and cultured for 2 days. Then, the cultured cells were again induced to differentiate with DMEM (containing 10% FBS) containing 1 μg/ml insulin, 0.5 mM IBMX, and 0.25 μM dexamethasone, and 2 days later, again It was cultured for 5 days by exchanging with DMEM containing insulin. After 5 days, it was replaced with a normal medium (DMEM, containing 10% FBS) and cultured while observing the cells until they changed morphologically to adipocytes.

Sudan III staining (S4136, sigma-aldrich) was performed using 3T3-L1 adipocytes, which have been differentiated as described above, in order to evaluate the efficacy of the cosmetic composition according to the present invention to inhibit fat accumulation in adipocytes. Adipocytes were fixed at room temperature with 4% paraformaldehyde (pH 7.2) in phosphate buffer, washed with PBS (phsphate buffered saline), and then stained with Sudan III, and then photographed for visual comparison. The control group used only the medium to which the composition of Examples or Comparative Examples was not added, and the other control group was treated with 50 μM of caffeine. The degree of fat accumulation inhibition was graded by dividing the staining degree into +++, ++, +, -. The results are shown in Table 3.

sample Inhibition rate (%) control +++ comparison group + Example 1 + Example 2 + Example 3 + Example 4 + Example 5 + Comparative Example 1 ++ Comparative Example 2 ++ Comparative Example 3 ++ Comparative Example 4 ++ Comparative Example 5 ++ Comparative Example 6 ++ Comparative Example 7 + Comparative Example 8 +

As shown in Table 3, Examples 1 to 5 of the present invention had an inhibitory effect on lipid synthesis, and it was found that Comparative Examples 1 to 6 were stronger. However, Comparative Examples 7 and 8 showed similar levels to Examples 1 to 5. Therefore, the composition according to an embodiment of the present invention can suppress the occurrence of acne by reducing sebum by inhibiting lipid synthesis.

3. Acne improvement, sebum secretion reduction, and stimulation test

A panel of 50 adult males and females in their 20s to 40s suitable for the purpose of the test through medical history and skin condition diagnosis was allowed to use the cosmetic compositions and erythrombomycin of Examples 1 to 5 and Comparative Examples 1 to 8 for one month. . Acne improvement scale was scaled from 1 to 5, with 1 being ‘not’, 3 points being ‘moderate’, and 5 points being ‘very much’. The experimental results are shown in Table 4 below as the average score of 50 participants.

Acne disappearance time was based on the number of days that the disappearance was read, and acne recurrence was based on the results after 1 month. Decreased sebum secretion was scored on a scale of 1 to 5, with 1 being ‘not’, 3 points being ‘moderate’, and 5 points being ‘very much’. The experimental results are shown in Table 4 below as the average score of 50 participants. The presence or absence of skin irritation was expressed as (number of persons who showed an irritant reaction)/(total number of test subjects).

Improves Inflammatory Acne When comedonal acne disappears acne recurrence Reduced sebum secretion with or without stimulation Example 1 4.1 3 days radish 4.2 0/50 Example 2 4.3 3 days radish 4.4 0/50 Example 3 4.0 3 days radish 4.0 0/50 Example 4 4.4 2 days radish 4.6 0/50 Example 5 4.7 2 days radish 4.7 0/50 Comparative Example 1 3.1 8 days radish 3.0 2/50 Comparative Example 2 3.2 8 days U 3.2 5/50 Comparative Example 3 2.8 9 days radish 2.6 4/50 Comparative Example 4 3.0 11 days U 2.8 8/50 Comparative Example 5 2.9 10 days radish 3.3 5/50 Comparative Example 6 3.3 9 days U 3.4 7/50 Comparative Example 7 4.3 3 days radish 4.4 0/50 Comparative Example 8 4.5 2 days radish 4.6 0/50 erythrombomycin 4.2 2 days radish 4.1 36/50

As shown in Table 4, in Examples 1 to 5, unlike Comparative Examples 2, 4, and 6, acne did not recur, Examples 1 to 5 were compared to Comparative Examples 1 to 6, and Example 4 was compared to Comparative Examples 1 to 6 Rather than 7, it was found that Example 5 had an overall better effect on acne improvement than Comparative Examples 1 to 8. On the other hand, in the case of erythrombomycin, an antibacterial standard material, although it showed efficacy similar to Examples 1 to 5, there was a concern of skin irritation for long-term use due to strong irritation in use.

4. Inflammation improvement effect

The anti-inflammatory effect was evaluated by the effect of inhibiting the production of prostaglandins. Effects were measured on macrophages using Examples 1 to 5 and Comparative Examples 1 to 8. First, the cyclooxygenase (COX) activity remaining in the cells was irreversibly inhibited by adding aspirin to the macrophages collected from the abdominal cavity of the mouse so that the final concentration was 500M. Then, 100 μl of the suspension was added to each well of a 96-well cell culture tube _{and cultured for 2 hours in an incubator under 5% CO 2} and 37° C. conditions to attach macrophages to the surface of the vessel. Then, the adherent macrophages were washed 3 times with PBS and then used to test the effect of the extract. ^{After culturing for 12 hours by adding RPMI medium containing 1% (w/v) LPS to 5x10 4} cells/ml of the cultured macrophages, the production of prostaglandins was induced, and the extract was treated with 100 μl to convert free prostaglandins into enzymes. It was quantified using an immunoassay (ELISA).

At this time, the prostaglandin production inhibitory activity of the extract was determined by setting the difference between the prostaglandins generated in the LPS-treated group and the non-treated group to 100%, and by treating the LPS and the sample together, the percentage of prostaglandins decreased through the control was compared and determined, and the results (the effect of inhibiting the production of prostaglandins) are shown in Table 5 below.

Blank 100% Control group (aspirin treatment group) 25.0% Example 1 31.1% Example 2 29.2% Example 3 32.5% Example 4 26.6% Example 5 24.5% Comparative Example 1 43.2% Comparative Example 2 41.2% Comparative Example 3 44.4% Comparative Example 4 38.3% Comparative Example 5 40.1% Comparative Example 6 39.8% Comparative Example 7 28.7% Comparative Example 8 26.2%

As shown in Table 5 above, as a result of the experiment, when Examples 1 to 3 were treated like the control group treated with aspirin, the inhibitory effect of prostaglandin production was greater than that of Comparative Examples 1 to 6, compared to Comparative Examples 1 to 6 when treated with Example 4 Compared to to 7, it was found that when Example 5 was treated, it was higher than Comparative Examples 1 to 8. Through this, it was found that the cosmetic composition according to an embodiment of the present invention can provide an excellent anti-inflammatory effect.

5. Skin soothing, moisturizing and elasticity improvement effect - Panel evaluation

The compositions (serums) of Examples 1 to 5 and Comparative Examples 1 to 8 were used for a panel of 50 adult men and women in their 20s to 40s suitable for the purpose of the test through a medical history examination and skin condition diagnosis to reduce waste, Soothing, moisturizing, and elasticity were evaluated.

Take an appropriate amount of the serum of Examples 1 to 3 and Comparative Examples 1 to 6, spread it on the nose and the skin around it, leave it for 10 to 15 minutes, and apply once a day for 4 weeks in a way that lightly washes the face with warm water According to the opinion of the panel, the degree of improvement was evaluated by a 5-step discrimination method (1: very bad, 2: bad, 3: normal, 4: good, 5: very good), and the average value is shown in Table 6 below.

division Moisturizing elasticity Calm Example 1 4.2 4.1 4.1 Example 2 4.3 4.3 4.2 Example 3 4.0 4.1 4.0 Example 4 4.5 4.6 4.4 Example 5 4.7 4.8 4.5 Comparative Example 1 3.2 3.3 2.9 Comparative Example 2 3.0 3.2 3.1 Comparative Example 3 3.1 3.0 3.1 Comparative Example 4 2.8 3.1 2.9 Comparative Example 5 3.2 2.9 3.1 Comparative Example 6 3.5 3.2 3.3 Comparative Example 7 4.4 4.3 4.1 Comparative Example 8 4.7 4.7 4.8

Referring to Table 6, the cosmetic composition of Examples 1 to 3 was better than the cosmetic composition of Comparative Examples 1 to 6, the cosmetic composition of Example 4 was higher than the cosmetic composition of Comparative Examples 1 to 7, and the cosmetic composition of Example 5 was It was confirmed that the moisturizing, elasticity, and soothing effects were overall superior to the cosmetic compositions of Comparative Examples 1 to 8.

Claims (4)

Dipotassium Glycyrrhizate 0.01 to 0.07 wt%, Camellia Oil 0.01 to 0.4 wt%, Epilobium Fleischeri Extract 0.05 to 0.25 wt%, Witch Hazel Extract Extract) 0.1 to 2% by weight, Willow Bark Extract 0.1 to 0.7% by weight, Camomile Extract 0.1 to 0.4% by weight, Vitamin B6/HCl 0.02 to 0.1% by weight, Niacin Amide ) 0.1 to 1% by weight, Methyl Sulfonyl Methane (MSM) 0.1 to 0.5% by weight, Salicylic Acid 0.04 to 0.2% by weight, Aloe Leaf Extract 0.1 to 2% by weight and glycol A cosmetic composition comprising 0.04 to 0.2% by weight of acid (Glycolic Acid).
delete The method according to claim 1, wherein 0.1 to 0.4% by weight of glucose and fructose mixture, 1 to 7% by weight of omija, ginger, yellow lily and green tea extract mixture, and 0.1 to 2% by weight of propolis and Machihyeon (Portulaca Oleracea) extract mixture is further added. comprising, a cosmetic composition.
The cosmetic composition of claim 1, wherein the composition is for improving acne.