KR101870780B1 - A feminine cleanser composition comprising roes damascena flower oil and propolis complex extracts - Google Patents

Abstract

One aspect of the present invention provides a feminine cleanser composition comprising damask rose flower oil and an extract of Calendula flower water and propolis as active ingredients. An object of the present invention is to provide the feminine cleanser composition which is less irritating to the skin and has excellent anti-inflammatory and antibacterial activity.

Description

TECHNICAL FIELD [0001] The present invention relates to a feminine cleaning composition comprising a damask rose flower oil and a propolis extract as an active ingredient.

The present invention relates to a cleansing composition for women containing natural ingredients.

The quality of the woman is the connection between the vulva and the uterus, which is the most humid and warm place in the female body. Women's vagina is a suitable environment for bacteria or fungi, which is the main cause of vaginitis, and the disease is likely to occur.

In normal conditions, many lactic acid bacteria live in the vagina, making the inside of the vagina mildly acidic to inhibit the growth of pathogens and fungi. However, the use of antibiotics or long-term use of birth control pills, tampons or loops, pregnancy or childbirth, When the balance inside the vagina breaks down due to hormonal balance changes, vaginitis may occur due to the proliferation of opportunistic pathogens that normally inhibit growth under normal conditions in the vagina.

Vaginitis has been reported as bacterial vaginosis, Candida vaginitis, Trichomonas vaginitis and Atrophic vaginitis.

The most common disease is bacterial vaginosis, caused by Gardnerella vaginalis . Candida albicans , common with bacterial vaginosis, is caused by the fungus Candida albicans .

To prevent female diseases, cleaners (vaginal cleansers) and vulva cleaners are often used. As a local cleaning agent commonly used to prevent vaginitis, a product obtained by sulfonating fatty acids or distilled lauryl alcohol and synthesizing emollients, thickeners, nutrients, foaming agents and organic disinfectants Are being sold.

However, the chemical quality detergent has a limitation in that the detergent effect is lowered and the germs are removed instead of removing the germs, and the odor and unpleasant odor removing effect are inhibited.

It is also known that polyene-based drugs such as nystatin, amphotericin B, azole-based drugs such as ketoconazole, miconazole, There are imidazole drugs and triazole drugs such as itraconazole and fluconazole but resistance to antibiotics may cause further difficulties in the subsequent treatment.

Although various drugs have been proposed as methods for treating vaginal cleansing and abnormal symptoms, it is still not enough to present a non-irritating detergent, a cleanser or a drug in consideration that the site of use is the most sensitive mucosal site for external substances . Thus, there is a need to develop new technologies to meet the ongoing need for cleaners using natural extracts without irritation and side effects.

Meanwhile, the cosmetics industry is developing a number of products using natural products to reduce skin irritation caused by various chemicals. In addition to low adverse effects on skin, natural materials have recently been increasingly developed as cosmetic raw materials, as consumers have become more receptive to cosmetics using natural materials.

The present inventors have developed an environmentally friendly and highly functional cleansing product for women, which contains only natural ingredients corresponding to EWG 1 grade and can safely use sensitive skin.

Korean Patent Publication No. 10-2001-0025970 Korean Patent Publication No. 10-2003-004587

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a cleaner composition for women which is less irritating to skin and has excellent anti-inflammatory and antibacterial activity.

In one aspect of the present invention, a multimask rose oil; And marigold flower juice and propolis extract as an active ingredient.

In one embodiment, the multi-mask rose oil may be a fermentation oil.

In one embodiment, the fermented oil can be obtained by inoculating Pseudozyma tsukubaensis .

In one embodiment, the feminine cleaner composition may further comprise a black tea extract.

In one embodiment, the feminine cleaner composition may further comprise at least one member selected from the group consisting of lavender foliage, bitter orange fountain, pine fountain, and elder fountain.

In one embodiment, the feminine cleaner composition comprises 10 to 30 parts by weight of the lavender flowers, 10 to 30 parts by weight of the bitter orange flowers, 10 to 30 parts by weight of the bitter orange flowers, and 10 to 30 parts by weight of the Elder flowers can do.

In one embodiment, the feminine cleaner composition is selected from the group consisting of softening longevity, convergent lotion, nutritional lotion, lotion, nutritional cream, massage cream, essence, cleansing cream, cleansing foam, cleansing water, pack, spray, and powder May be formulated into more than one.

The cleaning composition for women according to one aspect of the present invention has an excellent anti-inflammatory and antibacterial activity, and is excellent in cleansing the vulva, cooling, itching and odor, and is superior in the vaginitis-improving effect compared to the conventional vaginal cleansing composition for women.

In particular, the natural raw material applied to the female cleanser composition of the present invention is a raw material corresponding to EWG 1 grade and can be safely applied to sensitive skin without harmful to human body.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

According to one aspect of the present invention, And marigold flower juice and propolis extract as an active ingredient.

The above-mentioned " cleaner composition for women " is used for the purpose of preventing secretions, itching, smell, and the like, which are caused by infection by bacteria invading from the outside, and reducing or eliminating unpleasantness. The above-described cleaner composition for women may be effective in eliminating the causes of various gynecological diseases such as infertility, uterine myoma, uterine cancer and the like, which may be caused by cold infection, inflammation and itching as well as bacterial infection or inflammation.

The above-described cleaner composition for women can minimize the chemical preparation and replace the chemical agent with a natural-derived component, thereby realizing superior anti-inflammation and antimicrobial effect and hypoallergenic to the skin.

The above-mentioned " comprising as an active ingredient " may mean an effective amount which can exhibit a female cleansing effect, for example, an antibacterial, antiinflammatory, skin condition improvement and the like.

In particular, the above-described cleaner composition for women satisfies both functionality and safety since it contains only EWG level 1 components, and is easy to apply to sensitive skin because it is not harmful.

EWG is a cosmetic ingredient harmful grade announced by EWG (Environmental Working Group), a nonprofit environmental NGO in the United States. EWG carefully examines the harmfulness of cosmetic ingredients and classifies the hazard level from 1 to 10, and 1 Class 2 and Class 2 are classified as safety class.

The above-mentioned " Rosa damascena " is a plant of Rosaceae Rosaceae. Flowers, leaves, bark, branches, fruit or roots can be used as it is or in the form of pulverized powder.

The above-mentioned Rosa Damascena Flower Oil is a volatile oil extracted from a damask rose flower and added to impart fragrance. Like olivanium, it is extracted from a natural substance, The accumulation can be minimized to provide a fresher and refreshing feeling.

"Propolis" is a substance made by mixing bees with their own needles and enzymes in a resin-like material extracted from various plants for their survival and propagation. It contains a large amount of organic matter and minerals, and contains minerals, Vitamins, amino acids, fats, organic acids, and flavonoids play important roles in cell metabolism.

The propolis has been reported to have a therapeutic effect on skin diseases, hair loss suppression effect, cosmetic effect and bacterial dental disease caused by fungi such as anticancer, burning ability, eczema, atopic dermatitis and athlete's foot, Stimulating activity, treatment of scurvy by the action of vitamin p, hypotensive effect of hypertensive patients, balance of biohormones, and so on.

The "Marigold (Calendula officinalis "is a perennial plant originating in southern Europe and the Mediterranean. The marigold is abundant in lutein, and the flavonoids such as apigenin and its derivatives and rutin, coumarin compounds such as Umbelliferone, chamazulene and the like Volatile oil, which is effective for antibacterial, anti-inflammatory, pain-relieving and wound healing as well as for relieving inflammation of the skin and mucous membranes.

Lutein is a powerful antioxidant that acts as an antimicrobial, anti-microbial and anti-inflammatory agent. It belongs to xanthophylls in cartiloids and acts as a pigment for photosynthesis of plants. The marigold also contains flavonoids such as lycopene, violaxanthin, rubyxanthin, neo-lycopene A, citraxanthin and clavochrome, which promote the treatment of wounds caused by the skin, inhibit inflammation It has the effect of soothing sensitive skin.

The floral water is a water-soluble substance which is separated when the essential oil is extracted by the steam distillation method, and contains an essential oil component of about 0.002 to 0.005%, so that the effect of sterilization, disinfection and moisturizing is excellent. In addition, the flowering water is weakly acidic, having a pH of 2.9 to 6.5, is very stable, has little irritation to the skin, and is a component of each essential oil contained in natural raw materials, and has a unique fragrance and efficacy.

The damask rose oil may be a fermentation oil. Although the fermented oil contains a large amount of unsaturated fatty acid, it has excellent oxidation stability and the molecular weight of the hydrophobic substance becomes small due to fermentation, so that penetration into the skin is easy

The fermented oil is a Pseudozyma tsukubaensis ). < / RTI > It is known that the pseudosjimachu cabbans are produced by using an oil or the like as a carbon source to produce a biosurfactant upon fermentation, and the content of the unsaturated fatty acid in the multimask rose oil can be increased and oxidation stability can be effectively improved.

The ladies' cleanser composition may further comprise a black tea extract.

The term " black tea " is a kind of camellia sinensis which is more fermented than white tea, green tea and oolong tea. Therefore, the tea is strong in fragrance and contains more caffeine. In the Orient, tea is called red tea because the light is red. In the West, it is called 'black tea' because of the black color of the tea leaves.

The black tea promotes the oxidation and polymerization of polyphenols by rolling or crushing fresh tea leaves in the manufacturing process, and produces other polyphenols such as thearubigins and theaflavins, (Sun et al. 2006). The tea bulb and green tea flavonol have excellent antioxidant activity due to their radical scavenging ability and metal chelating function.

The above-mentioned "extract" refers to a solvent in which an effective ingredient contained in an extraction raw material has been transferred by contacting a solvent and an extraction raw material under specific conditions. If a substance is separated from a natural product, the extraction method, You can include them all regardless. For example, it may include extracts of components dissolved in a solvent from natural products by using water or an organic solvent, and specific components of natural products, such as oils obtained by extracting only specific components such as oil.

The extract may be extracted with at least one solvent selected from the group consisting of purified water, a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol, % Concentration (v / v) of ethanol.

The extraction ratio of the active ingredient contained in the raw material may be different depending on the polarity of the solvent. Since the ethanol is excellent in selectivity for extracting a physiologically active substance from a natural raw material, the ethanol extract can realize an optimal skin improving effect .

Particularly, the polarity of water and ethanol is different, so that the effective ingredient to be extracted according to each polarity can be changed, and the concentration of ethanol can be appropriately controlled so that an optimal skin improving effect can be realized. At this time, if the concentration of the ethanol exceeds 90%, an adequate yield may not be realized. If the concentration is less than 60%, the effective ingredient showing skin improving effect may not be extracted sufficiently.

The extract is washed with water, dried and crushed, and then extracted with a solvent having a volume of 8 to 12 times the weight of the raw material for about 1 to 24 hours by a conventional method such as circulation extraction, pressure extraction, And filtering. In addition, the extract can be obtained in powder form by an additional process such as vacuum distillation or freeze-drying.

The extract may also contain an extract which has been subjected to a conventional purification process. For example, the extract may be subjected to various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) Lt; RTI ID = 0.0 > fractions. ≪ / RTI >

In addition, the feminine cleaner composition may further include at least one member selected from the group consisting of lavender foliage, bitter orange fountain, pine fountain, and elder fountain.

The above-mentioned " Lavandula species " is an evergreen perennial plant with Lamiaceae. It is originated from the Mediterranean coast and is used for herbal tea, aromatherapy, ornamental. The lavender contains essential oils (fragrant oil) in leaves, flowers and stems. The lavender has a sterilization and insecticidal effect, restores fatigue, gives vitality, and has sterilization, disinfection and preservative action so as to sedate against troublesome skin.

The above-mentioned " Bitter Orange " is a fruit of citrus tree ( Citrus aurantium var . Amara ) and is known as a safe substance having high antibacterial activity and no toxicity, and is known to have anti-aging and skin cleansing effects.

The above-mentioned " Tiliaamurensis " is a deciduous broad-leaved arboreous tree whose leaves are almost circular, pointed with a pointed end, and have long petiole. The above-mentioned trees are mainly grown on hillside and valleys and distributed in various parts of Korea. The tree has been reported to have antipyretic, anticancer and anti-inflammatory effects.

The " Elder ( Sambucus canadensis ) is a deciduous broad-leaved shrub with a dead-necked tree. The Elder sp. Plants cause a pleasant smell by their volatile components. 79 compounds were identified from the steam distillation of the elder flower essential oil, and the main components were trans-3,7-dimethyl-1,3,7-octatrien-3-ol, palmitic acid, - hexenol and cis- and trans-rose oxides.

The above-described cleaner composition for women may contain 10 to 30 parts by weight of the lavender flowers, 10 to 30 parts by weight of the bitter orange flowers, 10 to 30 parts by weight of the bitter orange flowers, and 10 to 30 parts by weight of the Elder flowers.

When the deviation of the mixing ratio of the extract is large, skin improvement and cleansing effects derived from each oil may not be implemented properly, so that the mixing ratio can be appropriately controlled in consideration of the working environment and the quality of the final product. The extract may have an optimal skin improving and cleansing effect derived from the active ingredient in the above weight range.

The feminine cleansing composition may be formulated into one or more selected from the group consisting of softening agents, convergent lotion, nutritional lotion, lotion, nutritional cream, massage cream, essence, cleansing cream, cleansing foam, cleansing water, pack, spray, .

The feminine cleaner composition can be formulated by conventional methods in the art. The contents disclosed in Remington ' s Pharmaceutical Sciences, Mack Publishing Company, Easton PA in the formulation of the external preparation for skin can be referred to, and in the formulation of the cosmetic composition, International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995).

Specifically, the above-described cleansing composition for women can be prepared in a general emulsified formulation and a solubilized formulation. For example, lotion such as soft lotion or nutrition lotion; Lotion such as facial lotion, body lotion; Nutritional creams, creams, etc .; essence; Makeup ointment; spray; Gel; pack; A foundation such as a liquid type, a solid type or a spray type; powder; A cleansing cream, a cleansing lotion, or a cleansing agent such as a cleansing foam, a soap, a body wash, and the like, but is not limited thereto. In addition, the feminine cleansing composition may be formulated into ointments, patches, gels, creams or spray agents, but is not limited thereto.

The above-mentioned female cleansing composition may suitably contain other components in addition to the essential components in the respective formulations within the range not hindering the object of the present invention depending on the type of the formulation or the purpose of use.

The ladies' cleansing composition may comprise a conventional acceptable carrier and may suitably contain, for example, oils, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a colorant, an antiseptic, It is not.

The acceptable carrier may vary according to the formulation. For example, when formulated into ointments, pastes, creams or gels, there may be used an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide, Mixture can be used

When the female cleansing composition is formulated into a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as the carrier component. In the case of spraying, chlorofluoro Propellants such as hydrocarbons, propane, butane or dimethyl ether.

When the female cleansing composition is formulated into a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethylcarbonate, ethyl acetate, benzyl benzoate, , 1,3-butylglycol oil may be used, and in particular fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan may be used .

When formulated as a suspension, the above-described feminine cleansing composition may contain, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the above-described female cleanser composition is formulated into soap, it is preferable to use, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, an isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, Can be used.

The above-mentioned cleaner composition for women may be used in a variety of forms such as a lipid material, an organic solvent, a solubilizer, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, It is usually used in cosmetics, such as fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents Such as, for example, cosmetic or dermatological ingredients, such as, for example, cosmetic or dermatological ingredients.

However, the auxiliary agent and the mixing ratio thereof may be appropriately selected so as not to affect the desirable properties of the feminine cleaning composition according to the present invention.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Manufacturing example  One : Pudding  Manufacture of fermented oils

The fermentation medium (glucose 1.5% (v / v), sugar 1.5% (v / v), starch syrup 1.5% (v / v), yeast extract 0.3%) containing glucose, sugar, syrup and yeast extract v / v)) was used to prepare Pseudozyma tsukubaensis ) was prepared.

To the medium (10 mL) was added 10 mg of Pseudosgachicus varnish-dried substance and pre-cultivation was performed. A dried and pulverized multi-masked rose flower was added to a medium having the same composition as the fermentation medium, and the pre-culture solution was inoculated and fermented.

Pseudosgimachukubansis was cultured at 36.5 ℃ for 36 hours and glucose and sugar content were controlled to increase the growth rate of the strain during culture. After the fermentation was completed, the fermented samples were obtained by centrifugation and filtration, and the fermented milk of the multistage rose flower was squeezed by the compression method.

Manufacturing example  2: Preparation of lactic acid fermentation oil

Fermentation oil was prepared in the same manner as in Preparation Example 1, but lactic acid bacteria ( Lactobacillus casei ) was inoculated to prepare a fermented oil.

Example  And Comparative Example

Samples of Examples and Comparative Examples were prepared according to the compositions shown in Table 1 below in order to evaluate anti-inflammatory and antibacterial effects. Flowering and extracts for the preparation of the compositions were obtained commercially.

[Content (parts by weight)] division Example Comparative Example One 2 3 4 5 6 7 8 One 2 3 4 5 Da mask rose oil 40 - - 40 40 40 40 - 80 40 40 70 70 Damask rose flower
Fermentation oil (Preparation Example 1) - 40 - - - - - 40 - - - - - Damask rose flower
Fermentation oil (Preparation Example 2) - - 40 - - - - - - - - - - Marigold flower 20 20 20 15 10 10 10 10 - 40 - - - Propolis extract 20 20 20 15 10 10 10 10 - - 40 - - Black tea extract - - - 10 10 10 10 10 - - - 10 - Lavender flowers - - - - 10 - 2.5 2.5 - - - - 2.5 Bitter orange flowering - - - - - 5 2.5 2.5 - - - - 2.5 Bloom - - - - - 5 2.5 2.5 - - - - 2.5 Elder flowering - - - - - 2.5 2.5 - - - - 2.5

Experimental Example  1: Skin safety test

The toxicity of the samples of the Examples and Comparative Examples to the cells was measured. Samples of the examples and comparative examples were suspended in purified water to give concentrations of 100, 50 and 25 μg / mL, respectively, and cell viability was measured by the following method.

Cytotoxicity was performed by modifying the method of Mosmann to measure cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent. HDF (Human Dermal Fibroblast) cells were seeded in a 96-well plate at a concentration of 1 × 10 ⁴ cells / well and stabilized at 37 ° C and 5% CO ₂ for 24 hours.

Samples of the examples and comparative examples were treated for each concentration and cultured for 24 hours. After the medium was removed, MTT was treated at a concentration of 5 mg / mL and cultured for 2 hours. 100 μL of DMSO (dimethyl sulfoxide) was added, stirred for 10 minutes, and absorbance was measured at 540 nm.

As a result of the measurement, no cytotoxicity was observed at all concentrations of each sample. The results suggest that the composite composition is harmless to the human body and is excellent in safety.

Experimental Example  2: Evaluation of antibacterial activity

The antimicrobial activity of the female cleaner composition including the samples of the above Examples and Comparative Examples was confirmed by using the disk diffusion method.

Yeast strains caused by the target causes the fungus Candida albicans (Candida albicans ) and the common bacterium Gardnerella ( Gardnerella < RTI ID = 0.0 > vaginalis ) was used.

100 쨉 l of Candida albicans and Gardnerella vaginalis, which had been pre-cultured, were plated on a plate medium (Sabouraud Dextrose Agar. The compositions of the Examples and Comparative Examples were placed on a sterilized paper disk at a concentration of 5 mg / disc and placed on a medium.

Itraconazole, an antifungal agent for Candida albicans, and Ampicillin, an antibiotic for Gardnerella vaginalis were used as a positive control, and they were placed on a paper disk at a concentration of 100 μg / disk and then placed on a medium I left it.

After the plate culture was incubated at 28 ° C for 48 hours, the size (mm) of the growth inhibition zone (mm) was measured and the results are shown in Table 2 below.

division Candida albicans Guard Nella Trousers Positive control group 34 33 Example 1 26 24 Example 2 32 29 Example 3 28 27 Example 4 31 28 Example 5 32 30 Example 6 33 30 Example 7 34 33 Example 8 36 35 Comparative Example 1 17 16 Comparative Example 2 23 22 Comparative Example 3 22 23 Comparative Example 4 20 18 Comparative Example 5 21 20

Referring to Table 2, the compositions of Examples 1 to 8 showed strong growth inhibition effects against Candida albicans and Gardnerella vajinalis.

Particularly, the antifungal and antimicrobial effects were further enhanced when the composition was applied with Daemark Rosaceae fermentation oils (Examples 2 and 3) and black tea extract (Example 4).

In addition, the compositions (Examples 5 to 8) in which Lavender flowering, Bitter orange flowering, Pine flowering and Elder flowering were mixed showed remarkably excellent growth inhibition effect.

Experimental Example  3: Assessment of inhibition of microbial growth

Effects of Lactobacillus casei and Lactobacillus casei on the growth of Candida albicans and Gardnerella vaginalis , which are known as lactic acid bacteria that grow in female vagina, .

The presence of increased turbidity in the medium was confirmed and the IC ₅₀ (half maximal inhibitory concentration) value required to inhibit the antimicrobial activity and the microorganism by 50% was measured using the Broth Microdilution Susceptibility Test Respectively.

Specifically, each composition was diluted with Sabouraud Dextrose medium to a cell number of 2 × 10 ⁵ cells / ml, and the compositions of the examples and the comparative examples were treated at a concentration of 50 mg / ml in 96-wells. After incubation at 28 ° C for 24 hours, the absorbance was measured at a wavelength of 570 nm using a microplate reader (BIO-RAD) to determine IC ₅₀ values. The results are shown in Table 3 below.

division Survival rate (%) Lactobacillus casei Candida albicans Guard Nella Trousers Example 1 97 6.9 8.4 Example 2 98 5.7 7.6 Example 3 98 6.3 7.7 Example 4 97 5.4 6.5 Example 5 98 5.1 6.9 Example 6 97 4.6 5.6 Example 7 97 2.8 3.2 Example 8 98 1.3 1.4 Comparative Example 1 84 29.4 24.7 Comparative Example 2 90 21.8 20.1 Comparative Example 3 92 19.3 17.5 Comparative Example 4 88 20.5 18.2 Comparative Example 5 87 20.3 19.5

Referring to Table 3, the compositions of Examples 1 to 8 hardly affected the proliferation of lactobacillus casei, which is a lactic acid bacterium, but effectively inhibited the growth of Candida bacillus and Gardnerella vaginalis.

In particular, in Comparative Example 1 in which only damask rose oil was applied, the growth of Lactobacillus casei was partially affected, and the growth inhibitory effect on Candida albicans and Gardnerella vaginalis was somewhat insufficient.

The compositions of Examples 1 to 8 inhibited the growth of Candida bacillus and Gardnerella, but did not affect the growth of Lactobacillus casei. The results show that the microbial-specific antimicrobial effect of the compositions of Examples 1 to 8 Suggesting that it is very likely to be used as a female cleaner.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (7)

Damask rose flower oil; And
A marigold flower, a lavender flower, a black tea extract and a propolis extract as an active ingredient. The method according to claim 1,
Wherein the damask rose flower oil is a fermentation oil. 3. The method of claim 2,
Wherein the fermented oil is obtained by inoculating Pseudozyma tsukubaensis . delete The method according to claim 1,
Wherein the composition further comprises at least one member selected from the group consisting of Veterinary Orange, Bloom orange, Blooming orange, and Bitter orange. 6. The method of claim 5,
10 to 30 parts by weight of the bitter orange flowering, 10 to 30 parts by weight of the above-mentioned flowering water, and 10 to 30 parts by weight of the Elder flowering. The method according to any one of claims 1 to 3, 5, and 6,
Wherein the composition is formulated as at least one selected from the group consisting of a softening agent, a softening agent, a cosmetic lotion, a nourishing lotion, a lotion, a nutritional cream, a massage cream, an essence, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray and a powder.