KR101924822B1 - A feminine cleanser composition comprising lentil bean extract - Google Patents

Abstract

The present invention relates to a female detergent composition comprising a lentil soybean extract, a flower extract, and a natural vegetable oil as an active ingredient.

Description

A FEMININE CLEANER COMPOSITION COMPRISING LENTIL BEAN EXTRACT,

The present invention relates to a female detergent composition comprising a lentil soy extract.

HX Wang and 1 other. An antifungal peptide from red lentil seeds. peptides. 28 pp. 557-552. (2007)
The quality of the woman is the connection between the vulva and the uterus, which is the most humid and warm place in the female body. Women's vagina is a suitable environment for bacteria or fungi, which is the main cause of vaginitis, and the disease is likely to occur.

In normal conditions, many lactic acid bacteria live in the vagina, making the inside of the vagina mildly acidic to inhibit the growth of pathogens and fungi. However, the use of antibiotics or long-term use of birth control pills, tampons or loops, pregnancy or childbirth, When the balance inside the vagina breaks down due to hormonal balance changes, vaginitis may occur due to the proliferation of opportunistic pathogens that normally inhibit growth under normal conditions in the vagina.

Vaginitis has been reported as bacterial vaginosis, Candida vaginitis, Trichomonas vaginitis and Atrophic vaginitis.

The most common disease is bacterial vaginosis, caused by Gardnerella vaginalis . Candida albicans , common with bacterial vaginosis, is caused by the fungus Candida albicans .

To prevent female diseases, cleaners (vaginal cleansers) and vulva cleaners are often used. As a local cleaning agent commonly used to prevent vaginitis, a product obtained by sulfonating fatty acids or distilled lauryl alcohol and synthesizing emollients, thickeners, nutrients, foaming agents and organic disinfectants Are being sold.

However, the chemical quality detergent has a limitation in that the detergent effect is lowered and the germs are removed instead of removing the germs, and the odor and unpleasant odor removing effect are inhibited.

It is also known that polyene-based drugs such as nystatin, amphotericin B, azole-based drugs such as ketoconazole, miconazole, There are imidazole drugs and triazole drugs such as itraconazole and fluconazole but resistance to antibiotics may cause further difficulties in the subsequent treatment.

Although various drugs have been proposed as methods for treating vaginal cleansing and abnormal symptoms, it is still not enough to present a non-irritating detergent, a cleanser or a drug in consideration that the site of use is the most sensitive mucosal site for external substances . Thus, there is a need to develop new technologies to meet the ongoing need for cleaners using natural extracts without irritation and side effects.

Meanwhile, the cosmetics industry is developing a number of products using natural products to reduce skin irritation caused by various chemicals. In addition to low adverse effects on skin, natural materials have recently been increasingly developed as cosmetic raw materials, as consumers have become more receptive to cosmetics using natural materials.

The present inventors have developed an environmentally friendly and highly functional cleansing product for women, which contains only natural ingredients corresponding to EWG 1 grade and can safely use sensitive skin.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a female detergent composition which is less irritating to the skin and is excellent in skin soothing, skin moisturizing and anti-inflammatory effects.

In order to accomplish the above object, one aspect of the present invention provides a female detergent composition comprising a lentil extract, a flower extract, and a natural vegetable oil as an active ingredient.

In one embodiment, the flower extract is selected from the group consisting of cornflower flower extract, safflower extract, evening primrose oil extract, Nola daffodil extract, Foumas arnica extract, snow lotus extract, Kanto bloom extract, Ganoderma lucidum extract, chamomile extract and rose flower extract It may be more than one selected.

In one embodiment, the natural vegetable oil may be at least one selected from the group consisting of Eucalyptus leaf oil, Borage seed oil, Avocado oil, Western plum seed oil, Green tea oil and Camellia oil.

In one embodiment, the female detergent composition may comprise 0.005-30.0 wt.% Of the lentil soybean extract, 0.005-30.0 wt.% Of the flower extract, and 0.1-15.0 wt.% Of the natural vegetable oil, based on the total weight of the composition .

In one embodiment, the extract is selected from the group consisting of water, C ₁ to C ₄ anhydrous or lower alcohols, a mixed solvent of water and lower alcohols, acetone, 1,3-butylene glycol, ethyl acetate, chloroform Can be obtained by extraction with one or more solvents.

In one embodiment, the feminine cleanser composition is selected from the group consisting of softening longevity, convergent lotion, nutritional lotion, lotion, nutritional cream, massage cream, essence, cleansing cream, cleansing foam, cleansing water, pack, spray, and powder May be formulated into more than one.

According to another aspect of the present invention, there is provided a female cleaner patch comprising the female cleaner composition.

In one embodiment, the female cleaner patch may comprise from 0.001 to 10.0% by weight of the female cleaner composition based on total weight.

The female cleaner composition according to one aspect of the present invention can maximize skin soothing, moisturizing and anti-inflammatory effects.

In particular, the natural raw material applied to the female cleanser composition of the present invention is a raw material corresponding to EWG 1 grade and can be safely applied to sensitive skin without harmful to human body.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

Female cleaner composition

According to one aspect of the present invention, there is provided a female detergent composition comprising, as an active ingredient, a lentil soybean extract, a flower extract, and a natural vegetable oil.

The above-mentioned " female cleansing composition " is used for the purpose of preventing secretions, itching, smell, and the like, which are symptoms caused by bacteria infected by externally invading bacteria, and reducing or eliminating unpleasantness. The female cleanser composition may be effective in eliminating the causes of various women's diseases such as infertility, uterine myoma, uterine cancer and the like, which may be caused by cold infection, inflammation and itching as well as bacterial infection or inflammation.

The female cleaner composition minimizes the chemical preparation and replaces the chemical agent with a natural-derived component, thereby achieving superior anti-inflammation, antimicrobial effect, hypo hypoallergenic, sedative and moisturizing effect.

The above-mentioned " comprising as an active ingredient " may mean an effective amount which can exhibit a female cleansing effect, for example, an antibacterial, antiinflammatory, skin condition improvement and the like.

Particularly, since the female cleaner composition contains only EWG level 1 component, it satisfies both functionality and safety and is easy to apply to sensitive skin because it is not harmful.

EWG is a cosmetic ingredient harmful grade announced by EWG (Environmental Working Group), a nonprofit environmental NGO in the United States. EWG carefully examines the harmfulness of cosmetic ingredients and classifies the hazard level from 1 to 10, and 1 Class 2 and Class 2 are classified as safety class.

The above-mentioned " Lens culinaris " is a perennial soybean plant originating in the southern European Mediterranean coast. It is a convex lens shaped bean on both sides. It is also called lentil and it is edible. The lentils are rich in protein, high in calcium, and contain vitamin A, vitamin B, iron, sulfur, fiber, zinc, folic acid and antioxidants. It regulates unnecessary keratin and wastes left on the skin, regulates skin texture, and is effective in moisturizing skin.

The flower extract may be at least one selected from the group consisting of cornflower flower extract, safflower flower extract, evening primrose oil extract, Naphtha daffodil extract, Foucae arnica extract, snow lotus extract, Kanto flower extract, Ganoderma lucidum extract, chamomile extract and rose flower extract.

The " Centaurea " cyanus "is a plant of the genus Chrysanthemum and the flower of the waxy flower, and the extract of the waxy flower is often used as an astringent acidic lotion. When the eyes are tired or inflamed, the leaves of the waxy flower are used as eyedrops. In addition to its medicinal effect, it has excellent regenerating and antioxidant properties, it can make your skin healthier and smoother. It also gives vitality to the skin and has an effect on inflammation and red skin calming.

The above " Alcea rosea "is a perennial plant belonging to the genus Asia and originating in Asia. It can be used as a flower, leaf, and root, and has red, white, purple, It is effective to lower the fever, relax the bowel and the stomach, to control the lack of planting, to produce the blood mixed with the hysteritis, and especially the root of the flower of the safflower is effective for the poor circulation of women, great.

The " evening primrose ( Oenothera tetraptera "is an annual plant belonging to the genus North America, and belongs to the spider and evening primrose. The evening primrose is a herbaceous plant belonging to the genus Chrysanthemum, It is effective for diabetes, hyperlipidemia and obesity. Evening primrose contains a large amount of linolenic acid, gamma linolenic acid and other fatty acids, and it can improve blood circulation, physical function and physical fitness, Inhibition, skin itching, PMS syndrome (PMS), and skin health.

The above-mentioned " Narcissus Pseudo-narcissus L. " is originated in Europe and is a perennial plant of the field of daffodils. It is effective in treating swelling and arthritis.

The above-mentioned " Arnica montana L. " is a perennial plant of Asteraceae. Arnica flower extracts make your skin radiant and radiant, and give your weary skin a revival. It also has skin moisturizing, blood circulation, wound healing, pain relief, and anti-inflammation.

The " snow lotus ( Saussurea inovlucrata "is a flower bloomed in the snow, and is a flower of a chrysanthemum which grows in the cracks of the high mountains more than 3,000 to 4,000 meters above sea level. It contains acacetin which inhibits heart disease and arrhythmia and has excellent anticancer, antioxidant and antiallergic effect. Menstrual irregularities, poor circulation in the belly, infertility, menstrual irregularities, and aging.

The "Kanto bloom ( Farfarae flos ) "is a descendant of Asteraceae in the Kanto region ( Tussilago farfara ) buds. Kantouhwa is used mainly for shinhae, geumdam, sputum, pulmonary tuberculosis, and lung abscesses because it has a detoxification effect. But it also has skin improving effect such as anti-inflammation, antibacterial, antioxidant and wrinkle improvement.

The " Lonicera japonica Thunberg " is a flower of a perennial herbaceous plant belonging to the family Indanaceae. It has a unique flavor and contains ruriserin, flavoid, tannin, alkaloid, ruterinin, isosidol, saponin, lauricin, ruteorin and so on. Anti-bacterial, anti-inflammatory, antipyretic, increased leukocyte phagocytosis, central nervous stimulation, serum cholesterol lowering, ulcer prevention. It is also good for inflammation. It is easy to discharge boils, skin torn, poisonous organs, inflammation or aggravation of skin organs, skin tissue necrosis caused by hypertrophy, mastitis, colitis, gastric ulcer, cystitis, sore throat, tonsillitis, bronchitis, conjunctivitis, High fever due to mumps, and purulent infections.

The "Chamomile (Matricaria chamomilia ") is a perennial herbaceous plant belonging to the Asteraceae family originating in India and Europe, including Diez chamomile with yellow flowers, Roman chamomile with white flowers, German chamomile, and Bodegold chamomile. And is effective for the poor circulation of women, and is good for relieving low salt, preservative, anthelmintic and cramps.

The above-mentioned "Rosa" is a plant belonging to the genus Rosaceae, grown mainly for perfuming or medicinal purposes, and then cultivated for medicinal ornamental purposes. Red rose flowers are highly effective, and preferably Damascus roses can be used, but are not limited thereto. Rosaceae extract is a unique fragrance, has antidepressant effect, anti-inflammatory, sterilization, anti-convulsive, anti-virus, sterilization, hemostasis, diarrhea prevention and soothing effect. Especially, it has perfume and deodorant effect by the main ingredients such as geraniol, it helps ovarian secretion of estrogen hormone, so it relieves symptoms caused by female hormone imbalance and has excellent effect of restoring skin elasticity.

The above-mentioned "extract" refers to a solvent in which an effective ingredient contained in an extraction raw material has been transferred by contacting a solvent and an extraction raw material under specific conditions. If a substance is separated from a natural product, the extraction method, You can include them all regardless. For example, it may include extracts of components dissolved in a solvent from natural products by using water or an organic solvent, and specific components of natural products, such as oils obtained by extracting only specific components such as oil.

The extract may be extracted with at least one solvent selected from the group consisting of purified water, a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol, % Concentration (v / v) of ethanol.

The extraction ratio of the active ingredient contained in the raw material may be different depending on the polarity of the solvent. Since the ethanol is excellent in selectivity for extracting a physiologically active substance from a natural raw material, the ethanol extract can realize an optimal skin improving effect .

Particularly, the polarity of water and ethanol is different, so that the effective ingredient to be extracted according to each polarity can be changed, and the concentration of ethanol can be appropriately controlled so that an optimal skin improving effect can be realized. At this time, if the concentration of the ethanol exceeds 90%, an adequate yield may not be realized. If the concentration is less than 60%, the effective ingredient showing skin improving effect may not be extracted sufficiently.

The extract is washed with water, dried and crushed, and then extracted with a solvent having a volume of 8 to 12 times the weight of the raw material for about 1 to 24 hours by a conventional method such as circulation extraction, pressure extraction, And filtering. In addition, the extract can be obtained in powder form by an additional process such as vacuum distillation or freeze-drying.

The extract may also contain an extract which has been subjected to a conventional purification process. For example, the extract may be subjected to various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) Lt; RTI ID = 0.0 > fractions. ≪ / RTI >

The natural vegetable oil may be at least one selected from the group consisting of Eucalyptus leaf oil, Borage seed oil, Avocado oil, Western plum seed oil, Green tea oil and Camellia oil.

The above-mentioned " Eucalyptus L. " is a herbaceous plant belonging to the Plants Department of Origin, Australia. Eucalyptus contains 1,8-cineol, pinene, aromadendrene, cuminaldehyde, pinocarveol, and rutin as active ingredients. Eucalyptus contains 1,8-cineol, pinene, aromadendrene, cuminaldehyde, pinocarveol and rutin. It can be used as a cleansing agent for skin ulcers and sores, as well as antimicrobial action that inhibits benign germs and detoxifies toxins. It also has antiparasitic, deodorizing and analgesic effects. The fragrance of Eucalyptus leaves calms the fatigue and negative stress, refreshes the mood, and has the effects of mood and emotional stability.

The " Borage seed " is the seed of Borage officinalis . Contains a large amount of GLA, potassium, calcium, and special fatty acids as effective ingredients. It is effective in improving skin smoothness and suppleness, and cleansing and cleansing the skin. It also has the effect of calming skin irritation caused by external stimuli and harmful substances, restoring circulation of the skin cycle and giving elasticity and energy to tired skin.

The "avocado (Avocado)" has haejugo discharged by the sodium rich in potassium and contains a blood pressure rise suppressing fiber, vitamin 11 kinds of mineral species, including 14, as an active ingredient. In particular, vitamin E, which is a fat-soluble vitamin, called tocopherol, is abundant and has effects such as suppression of aging, skin moisturization, removal of wrinkles, eczema relief, and image healing.

The "plum seed" is plum (Prunus domestica . It contains polyphenol component, vitamin A and vitamin E, and has effects such as skin beauty, aging suppression, menopausal disorder, and skin elasticity.

The above " Green tea " contains vitamin A and tannin as active ingredients. When vitamin A is lacking, the skin becomes rough, the luster disappears, and the mucous membrane becomes weak. Vitamin A, vitamin B2 and carotene are effective to soothe skin and soothe skin. Vitamin C inhibits the deposition of melanin pigment and keeps the skin whitened by preventing pigmentation of spots and freckles. . The catechin ingredient has sebum control and bactericidal action to maintain smooth skin. Tocopherol, amino acid protects skin from weakness, prevents oxidation of cell membranes, inhibits aging, and moisturizes skin. .

The "japanese camellia" is an evergreen tree native to southern Korea, Japan, and China. Most make camellia oil or oil from seeds or seeds. Camellia oil contains oleic acid in abundance and has effects such as low irritation, skin moisturization, antioxidation, skin soothing, whitening, anti-wrinkle and itching relief.

The female detergent composition may comprise from 0.005 to 30.0% by weight of the lentil soybean extract, from 0.005 to 30.0% by weight of the flower extract, and from 0.1 to 15% by weight of the natural vegetable oil, based on the total weight of the composition.

When the content of the lentil soybean extract is less than 0.005% by weight, the skin soothing, moisturizing and anti-aging effect by the lentil soybean extract may not be properly realized. When the content is more than 30.0% by weight, The inherent quality may deteriorate.

When the flower extract is less than 0.005% by weight, the skin moisturizing, emotional stability and anti-inflammatory effect by the flower extract may not be properly realized. When the flower extract is more than 30.0% by weight, the specific weight of the other additives required for commercialization is decreased. The quality may be deteriorated.

When the natural vegetable oil is less than 0.1% by weight, emotion stability, fragrance, skin soothing and moisturizing effect may not be realized properly. If the natural vegetable oil is more than 15.0% by weight, the specific gravity of the other additives required for commercialization is decreased. Can be degraded.

When the variation of the mixing ratio of the extract is large, the skin moisturizing, emotional stability and cleansing effects derived from each oil may not be properly realized. Therefore, the mixing ratio can be appropriately controlled in consideration of the working environment and the quality of the final product have. The extract may have an optimal skin moisturizing, anti-inflammatory and cleansing effect derived from the active ingredient in the above weight range.

In addition, since the natural raw material contains only the EWG level 1 component, it satisfies both functionality and safety and is easy to apply to sensitive skin because it is not harmful.

EWG is a cosmetic ingredient harmful grade announced by EWG (Environmental Working Group), a nonprofit environmental NGO in the United States. EWG carefully examines the harmfulness of cosmetic ingredients and classifies the hazard level from 1 to 10, and 1 Class 2 and Class 2 are classified as safety class.

The female cleaner composition can be formulated by conventional methods in the art. The contents disclosed in Remington ' s Pharmaceutical Sciences, Mack Publishing Company, Easton PA in the formulation of the external preparation for skin can be referred to, and in the formulation of the cosmetic composition, International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995).

Specifically, the female detergent composition can be prepared into a general emulsified formulation and a solubilized formulation. For example, lotion such as soft lotion or nutrition lotion; Lotion such as facial lotion, body lotion; Nutritional creams, creams, etc .; essence; Makeup ointment; spray; Gel; pack; A foundation such as a liquid type, a solid type or a spray type; powder; A cleansing cream, a cleansing lotion, or a cleansing agent such as a cleansing foam, a soap, a body wash, and the like, but is not limited thereto. In addition, the feminine cleansing composition may be formulated into ointments, patches, gels, creams or spray agents, but is not limited thereto.

The female cleansing composition may suitably contain other ingredients in addition to the above-mentioned essential ingredients in each formulation within the range not impairing the object of the present invention depending on the type of the formulation or the purpose of use.

The female cleansing composition may comprise a conventional acceptable carrier and may include, but is not limited to, oils, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, It is not.

The acceptable carrier may vary according to the formulation. For example, when formulated into ointments, pastes, creams or gels, there may be used an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide, Mixture can be used

When the female cleansing composition is formulated into a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as the carrier component, Propellants such as hydrocarbons, propane, butane or dimethyl ether.

When the female cleansing composition is formulated into a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, , 1,3-butylglycol oil may be used, and in particular fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan may be used .

When formulated as a suspension, the female detergent composition may contain, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the female detergent composition is formulated into a soap, it is preferable to use as the carrier component an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, an isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, Can be used.

The female cleansing composition may be a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, It is usually used in cosmetics, such as fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents Such as, for example, cosmetic or dermatological ingredients, such as, for example, cosmetic or dermatological ingredients.

However, the auxiliary agent and the mixing ratio thereof can be appropriately selected so as not to affect the desirable properties of the female cleaner composition according to the present invention.

Female Cleaner Patch

Another aspect of the present invention provides a female cleaner patch comprising the female cleaner composition. The female cleaner patch may comprise from 0.001 to 10.0% by weight, preferably from 0.01 to 5.0% by weight, of the female cleaner composition based on total weight.

If the content of the female cleaner composition is less than 0.001 wt%, the effect thereof is difficult to expect. If the content of the female cleaner composition is more than 10.0 wt%, the palatability to the fragrance may be deteriorated.

The female cleaner patch can be manufactured by a conventional method. In the method of manufacturing the patch, the contents disclosed in Korean Patent No. 10-1149813 and Korean Patent No. 10-1563323 can be referred to.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Preparation Example 1: Preparation of lentil soybean extract

100 g of lentil bean dry weight of EWG 1 grade was put into a flask and extracted with 1,000 g of extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare an extract.

Production Example 2: Preparation of flower extract

100g dry weight of EWG 1st class cornflower, flower of safflower, evening primrose, narcissus flower, fountain arnica, snow lotus flower, Kantou flower, gold eurow flower, chamomile and rose flower are put into a flask. Respectively. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare respective extracts.

Examples and Comparative Examples

To evaluate the anti-inflammatory and antibacterial effects, samples of Examples and Comparative Examples were prepared according to the composition shown in Table 1 below. Natural vegetable oils for the preparation of compositions were obtained commercially.

[Content (parts by weight)] division Example Comparative Example One 2 3 4 5 6 7 One 2 3 4 5 Lentil bean extract 12 20 20 20 5 5 5 80 - - - - Cornflower flower extract 5 3 6 - 6 12 - - 8 - 40 - Safflower extract 5 3 6 - 6 12 - - 8 - - - Evening primrose extract 5 3 6 - 6 12 - - 8 - - - Daffodil extract 5 3 6 - 6 12 - - 8 - - - Fountain Arnica extract 5 3 6 - 6 12 - - 8 - - - Snow lotus extract 5 3 - 6 6 - 12 - 8 - - - Kanto flower extract 5 3 - 6 6 - 12 - 8 - - - Gold Euphoria extract 5 3 - 6 6 - 12 - 8 - - - Chamomile extract 5 3 - 6 6 - 12 - 8 - - - Rose flower extract 5 3 - 6 6 - 12 - 8 - 40 - Eucalyptus leaf oil 3 5 10 - 2.5 5 - - - 13 - 40 Borage seed oil 3 5 10 - 2.5 5 - - - 13 - - Avocado oil 3 5 10 - 2.5 5 - - - 13 - - Western plum seed oil 3 5 - 10 2.5 - 5 - - 13 - - Green tea oil 3 5 - 10 2.5 - 5 - - 14 - - Camellia oil 3 5 - 10 2.5 - 5 - - 14 - 40

Experimental Example 1: Skin safety test

The toxicity of the samples of the Examples and Comparative Examples to the cells was measured. Samples of the examples and comparative examples were suspended in purified water and prepared by concentration (100, 50, 25 μg / mL), and cell viability was measured by the following method.

Cytotoxicity was performed by modifying the method of Mosmann to measure cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent. HDF (Human Dermal Fibroblast) cells were seeded in a 96-well plate at a concentration of 1 × 10 ⁴ cells / well and stabilized at 37 ° C and 5% CO ₂ for 24 hours.

Samples of the examples and comparative examples were treated for each concentration and cultured for 24 hours. After the medium was removed, MTT was treated at a concentration of 5 mg / mL and cultured for 2 hours. 100 μL of DMSO (dimethyl sulfoxide) was added thereto, and the mixture was stirred for 10 minutes and absorbance was measured at 540 nm.

As a result of the measurement, no cytotoxicity was observed at all concentrations of each sample. The results suggest that the composite composition is harmless to the human body and is excellent in safety.

Experimental Example 2: Evaluation of antibacterial activity

The antimicrobial activity of the female cleanser compositions including the samples of the above Examples and Comparative Examples was confirmed by using the disk diffusion method.

Yeast strains caused by the target causes the fungus Candida albicans (Candida albicans ) and the common bacterium Gardnerella vaginalis were used.

Candida albicans and Gardnerella vaginalis, which had been pre-cultured, were plated on a plate medium (Sabouraud Dextrose Agar. The compositions of the Examples and Comparative Examples were placed on a sterilized paper disk at a concentration of 5 mg / disc and placed on a medium.

Itraconazole, an antifungal agent for Candida albicans, and Ampicillin, an antibiotic for Gardnerella vaginalis were used as a positive control, and they were placed on a paper disk at a concentration of 100 μg / disk and then placed on a medium I left it.

After the plate culture was incubated at 28 ° C for 48 hours, the size (mm) of the growth inhibition zone (mm) was measured. The results are shown in Table 2 below.

division Candida albicans Guard Nella Trousers Positive control group 35 33 Example 1 32 33 Example 2 28 27 Example 3 26 25 Example 4 27 27 Example 5 25 24 Example 6 25 25 Example 7 26 24 Comparative Example 1 24 23 Comparative Example 2 19 22 Comparative Example 3 22 21 Comparative Example 4 20 19 Comparative Example 5 21 20

Referring to Table 2, the compositions of Examples 1 to 7 showed strong growth inhibition effects against Candida albicans and Gardnerella vajinalis.

Especially, when the whole extract of Lentil, flower extract, and natural vegetable oils were applied, the antifungal and antibacterial effect was further increased. Compared with Examples 2 to 4 and Examples 5 to 7, Comparative Example 1 and Comparative Examples 2 to 5, the higher the content of lentil soybean extract, the better the effect of inhibiting growth.

Experimental Example 3: Evaluation of microbial growth inhibiting ability

Effects of Lactobacillus casei and Lactobacillus casei on the growth of Candida albicans and Gardnerella vaginalis , which are known as lactic acid bacteria that grow in female vagina, .

The presence of increased turbidity in the medium was confirmed and the IC ₅₀ (half maximal inhibitory concentration) value required to inhibit the antimicrobial activity and the microorganism by 50% was measured using the Broth Microdilution Susceptibility Test Respectively.

Specifically, each composition was diluted with Sabouraud Dextrose medium to a cell number of 2 × 10 ⁵ cells / ml, and the compositions of the examples and the comparative examples were treated at a concentration of 50 mg / ml in 96-wells. After incubation at 28 ° C for 24 hours, the absorbance was measured at a wavelength of 570 nm using a microplate reader (BIO-RAD) to determine IC ₅₀ values. The results are shown in Table 3 below.

division Survival rate (%) Lactobacillus casei Candida albicans Guard Nella Trousers Example 1 98 2.9 3.3 Example 2 97 3.3 3.8 Example 3 98 4.3 4.6 Example 4 98 4.4 4.7 Example 5 97 3.8 4.1 Example 6 96 4.2 5.0 Example 7 96 4.3 4.9 Comparative Example 1 90 19.9 19.8 Comparative Example 2 87 20.7 21.3 Comparative Example 3 88 20.8 21.6 Comparative Example 4 84 25.7 26.3 Comparative Example 5 83 24.3 25.9

Referring to Table 3, the compositions of Examples 1 to 7 hardly affected the proliferation of lactobacillus casei, which is a lactic acid bacterium, but effectively inhibited the growth of Candida bacillus and Gardnerella vaginalis.

In Comparative Examples 2 to 5 in which lentil beans were not applied, the growth of Lactobacillus casei was partially affected and the growth inhibitory effect on Candida albicans and Gardnerella vaginalis was somewhat insufficient.

The compositions of Examples 1 to 7 inhibited the growth of Candida bacillus and Gardnerella, but did not affect the growth of Lactobacillus casei. The results showed that the compositions of Examples 1 to 7 had a microbial- Suggesting that it is very likely to be used as a female cleaner.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (8)

Lentil bean extract; Flower extracts, including Naphtho daffodil extracts and Foals arnica extract; And a natural vegetable oil containing borage seed oil as an active ingredient,
A female detergent composition having inhibitory activity against Candida albicans and Gardnerella vaginalis . delete delete The method according to claim 1,
0.005-30.0 wt.% Of the lentil soybean extract, 0.005-30.0 wt.% Of the flower extract, and 0.1-15.0 wt.% Of the natural vegetable oil, based on the total weight of the composition. The method according to claim 1 or 4,
The extract is extracted with water, at least one solvent selected from the group consisting of C ₁ to C ₄ anhydrous or lower alcohols, a mixed solvent of water and lower alcohols, acetone, 1,3-butylene glycol, ethyl acetate and chloroform ≪ / RTI > The method according to claim 1 or 4,
Wherein the female cleaner composition is formulated as at least one selected from the group consisting of softening agents, convergent lotion, nutritional lotion, lotion, nutritional cream, massage cream, essence, cleansing cream, cleansing foam, cleansing water, pack, spray, A cleaner composition for women. A patch of a female cleaner comprising the female cleaner composition of claim 1 or 4. 8. The method of claim 7,
A patch of a female cleaner comprising 0.001 to 10.0% by weight of said female cleaner composition based on total weight.