KR101542490B1 - Cosmetic composition comprising the mixed extracts of Achillea, Borage, Helichrysum, Lady's mantle, and Eucalyptus - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising as an active ingredient an extract of Jahrus, Borage, Everlasting, Lady's mantle, and Eucalyptus fermented extract obtained by fermenting Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus, Fermented by fermenting microorganism Saccharomyces cerevisiae on the extracts of Lactobacillus, Lactobacillus, Lactobacillus, Lactobacillus, Borage, Everlasting, Lady's mantle and Eucalyptus, And to provide a cosmetic composition containing the extract.

Description

[0001] The present invention relates to a cosmetic composition comprising a mixed extract composed of lauric acid, lauric acid, lauric acid, lauric acid, lauric acid, lauric acid,

The present invention relates to a cosmetic composition comprising as an active ingredient a mixed extract composed of Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus. More particularly, the present invention relates to a cosmetic composition comprising Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus The present invention relates to a cosmetic composition comprising fermenting microorganism Saccharomyces cerevisiae and containing the microorganism Saccharomyces cerevisiae.

Patent Document: Korean Application No. 10-2001-0022014

Accordingly, the present inventors have found a method capable of obtaining an effective ingredient at the highest possible yield in comparison with the above-mentioned prior arts and have applied it to cosmetics. As a result, the inventors of the present invention have found that the extract of Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus fermentation The present invention provides a cosmetic composition which exhibits an antioxidative effect and an inflammatory reaction inhibitory effect of the present invention by enriching an active ingredient rich in peptides and the like in a rich manner.

The objects and advantages of the present invention will become more apparent from the following detailed description of the invention when taken in conjunction with the accompanying drawings.

In order to accomplish the above object, the present invention provides a cosmetic composition comprising a fermented product of Yarrow, Borage, Everlasting, Lady's mantle, Eucalyptus, Lt; / RTI >

Preferably, the fermentation is carried out at a pH of 5 to 7 under a temperature condition of 20 to 40 DEG C in a bacterium selected from the group consisting of Yarrow, Borage, Everlasting, Lady's mantle, Eucalyptus, Yeast, Lactobacillus, Bacillus, And fermenting the mixture for 1 to 7 days.

Further, preferably, the cosmetic composition is characterized in that the yeast is Saccharomyces cerevisisae.

Preferably, the extraction process is carried out at a temperature of 40 to 90 DEG C for 1 to 24 hours

To obtain a cosmetic composition.

Preferably, the extraction is carried out using a solvent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane, And then extracting the mixture by using the cosmetic composition.

Preferably, the Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus fermentation extract are contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

In addition, the cosmetic composition preferably has an antioxidative effect and an inflammatory reaction inhibitory effect.

The cosmetic composition according to the present invention contains an effective ingredient of a jarow, borage, everlasting, lady's mantle, and eucalyptus fermented extract, and has no skin irritation, and has solubility, affinity and stability for various bases and additives for cosmetics, And the skin elasticity improvement effect and the inflammation reaction inhibition effect were particularly excellent.

The present invention provides a cosmetic composition comprising a Yarrow, Borage, Everlasting, Lady's mantle, and Eucalyptus fermented extract obtained by fermenting Yarrow, Borage, Everlasting, Ladies Mantle and Eucalyptus, followed by extraction and filtration.

According to the present invention, the fermented extract of Yarrow, Borage, Everlasting, Ladies Mantle and Eucalyptus has an increased peptide content as an active ingredient, and has excellent antioxidant activity (Experimental Example 3) and inflammatory response inhibitory effect (Experimental Example 4).

Therefore, the cosmetic composition of the present invention comprising the active ingredient of the various ingredients such as Yarrow, Borage, Everlasting, Lady's mantle, and Eucalyptus fermented extract has a high content of peptide as an active ingredient and has antioxidative and inflammatory reaction inhibitory effects Can be usefully used.

FIG. 1 is a graph showing the antioxidative effect according to the concentration of fermented extract of Yarrow, Borage, Everlasting, Ladies' Mantle and Eucalyptus according to the present invention,
FIG. 2 is a graph showing the inflammatory response inhibitory effect according to the concentration of the fermented extract of Yarrow, Borage, Everlasting, Ladies' Mantle, and Eucalyptus according to the present invention,
FIG. 3 is a graph showing inhibitory effect on melanin synthesis according to the concentration of fermented extract of Yarrow, Borage, Everlasting, Ladies Mantle and Eucalyptus according to the present invention

The present invention relates to a cosmetic composition containing a Yarrow, Borage, Everlasting, Lady's mantle, and Eucalyptus fermented extract obtained by fermenting, extracting and filtering Elaurium, Borage, Everlasting, Ladis mantle and Eucalyptus, Fermented with a fermenting microorganism selected from the group consisting of yeasts, lactic acid bacteria, bacteria of the genus Bacillus, and mixtures thereof, and extracting and filtering the yeast, barley, A cosmetic composition containing Everlasting, Lady's mantle, and Eucalyptus fermented extract as active ingredients was developed.

Achillea Millefolium is originated from Eurasia and purified in North America. It is now found in most of the world's temperate regions. Jarrow, called jelly, is one of the herbs that has been recognized as an excellent effect for wound healing, and has a nickname as a carpenter's herb because it hears wounds injured by tools such as saws, knives, and sickles. It was also said that during the First World War there was sterilization, hemostasis, and convergence, and wound treatment was performed using Yarrow, which was on the soldiers' side.

Borage is a legend that the Mediterranean coast is its birthplace, and it is a herb of geological and biennials and has given the liquor to soothe troubled soldiers in ancient times. The Borage leaves have a similar aroma to cucumber, It is known to be excellent in eczema, dermatitis, analgesia, fatigue recovery, cleansing, and cleansing because of its high content of calcium and potassium and excellent diuretic, analgesic, sweating, cleansing and skin softening action. Borage oil is extracted by steam distillation of oils from flowers and leaves, and the content of essential fatty acids is particularly abundant, among which gamma linolenic acid (GLA) contains more than twice the amount of evening primrose oil. Gamma linolenic acid plays an important role in the development and suppression of inflammation because it involves intracellular synthesis of ecosaind containing cytokines or prostaglandins, which are important for inflammation mediators. Borage oils are rich in gamma linolenic acid, There is an excellent effect on dermatitis. Borage oil is an oil that is used in facial treatments to give vitality, shine and elasticity to the skin. It helps the treatment of eczema and antimicrobial action and stimulates hormone secretion, which helps to relieve menstrual syndrome and chest pain. It is known.

Everlasting is called "Immortelle", which means "Everlasting" and is the scientific name Helichrysum or "eternal immortality" originating in parts of Asia close to North Africa, Crete and the Mediterranean Sea. Also. Especially Everlasting Oil is a valuable body treatment that has been used from ancient times with Frankinsense, Spikenard and Roses, and is said to have been used as a valuable oil to heal wounds in the mind.

Lady's mantle is a perennial plant belonging to the genus Alchemilla (Rosaceae), especially A. vulgaris. Alkemilla bulgaris is widespread in the temperate zone of the Northern Hemisphere and grows up to about 60 cm tall. The leaf is about 10㎝ wide and runs on a long stem, its shape is round and shallowly divided, and the edge has sawtooth. The flower is small, greenish-yellow and clusters at the end of the stem. The sharp young leaves of the lady's mantle are blended into Swiss tea and added to the salad. Leaves of green leaves reduce vaginal itching and help recovery after childbirth. It is known to regulate menstruation and slow down menopause.

Eucalyptus (Eucalyptus) is an evergreen tree, usually about 10 meters high, sometimes larger than 15 meters. Leaves are lanceolate with a length of 30cm and covered with white powder and perfume. The flower is rusty white and blooms in spring. The flowers have many operations, which extend out of the flower, with a well-developed flower bud at the bottom of the calyx tube. The fruit is the development of this flower jaw, and the seed is made in the fan groove of the fruit. Australia is the origin, and leaves use eucalyptus oil.

The cosmetic composition of the present invention as described above is a cosmetic composition containing a Yarrow, Borage, Everlasting, Lady's mantle, and Eucalyptus fermented extract obtained by fermenting Yarrow, Borage, Everlasting, Ladies Mantle, Eucalyptus, to be.

The cosmetic composition of the present invention composed of the above components is suitable as a cosmetic composition for skin antioxidant effect, inflammatory reaction inhibitory effect, melamine synthesis inhibitory effect and the like.

The inventors of the present invention have found that it is possible to obtain the components of the peptides having various potencies, in particular the filtration process, in order to find a method which can be obtained at the highest possible yield, such as Yarrow, Borage, Everlasting, Ladysmant, Eucalyptus After the fermentation, it was confirmed that the content of the active ingredient was increased through extraction and filtration processes, and it was confirmed that skin elasticity improvement effect and inflammation reaction inhibition effect were obtained by applying it to cosmetics, thereby completing the present invention.

In the cosmetic composition according to the present invention, the Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus may contain 1 to 30 parts by weight of Yarrow, 1 to 30 parts by weight of Borage, 1 to 30 parts by weight of Everlasting, 1 to 30 parts by weight of mantle, and 1 to 30 parts by weight of eucalyptus. The fermented extract consisting of the combination of these components is characterized in that it comprises Yarrow, Borage, Everlasting, Lady's mantle, Eucalyptus, Yeast, Lactobacillus, , Which can be obtained by fermentation for 1 to 7 days while maintaining a pH of 5 to 7 under a temperature condition of 20 to 40 ° C, more preferably using Saccharomyces cerevisisae And most preferably, Saccharomyces cerevisiae strain KCTC 7904 is used for fermentation It is.

The Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus used in the present invention are generally manufactured by Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus manufactured by a known method, It was made finely using 300 mesh. This was added to the cultivation medium of Saccharomyces cerevisiae (Saccharomyces cerevisiae KCTC 7904) to give 100 g / L of Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus. 1 to 4% of glucose, preferably 1 to 2%, is added as a carbon source, and 0.1 to 1% of peptone is added, preferably 0.2 to 0.5% is further added. The culturing was carried out using a 5 L fermenter at a temperature of 20 to 40 ° C for 1 to 7 days while maintaining the pH at 5 to 7. After culturing, the culture was centrifuged to remove the cultures first.

Preferably, the extraction is carried out at a temperature of 40 to 90 DEG C for 1 to 24 hours, preferably at a temperature of 40 to 60 DEG C for 12 to 24 hours, most preferably at a temperature of 50 DEG C Treat for 24 hours.

Also preferably, the extraction is carried out in an extraction solvent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane, , More preferably extracted with ethanol, 70% ethanol aqueous solution or water, most preferably with 70% ethanol aqueous solution.

Ethanol was added to the extract so as to be a final 70% (v / v) ethanol aqueous solution, and the mixture was refluxed three times for 5 hours, cooled, and washed with Whatman # 2 filter paper. When the filtration is completed, the extract obtained in the extracting step is transferred to the concentration tank, concentrated under reduced pressure, and lyophilized. Concentration under reduced pressure was performed using a rotary vacuum concentrator. Concentration conditions were as follows: 700 ml of the extract was put into a 1,000 ml round-bottomed flask, and the mixture was concentrated under reduced pressure for 2 hours while rotating at a temperature of from 650 to 1200 rpm at a temperature of from 650 to 750 mmHg. The concentrate was concentrated under reduced pressure, cooled to -40 ° C, maintained at -40 ° C for 2 to 4 hours, then dropped to -80 through trap freezing, kept at 10-30 mTorr for 120 minutes Lt; / RTI > Then, the temperature was maintained at -20 ° C for 240 minutes, at 0 ° C for 40 minutes, at 15 ° C for 340 minutes, at 30 ° C for 130 minutes, and then maintained at 1300 minutes until completely dried.

For reference, Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus fermented extract are extracts obtained by ultra-high pressure treatment of fermentation products obtained by fermentation of Yarrow, Borage, Everlasting, Ladis mantle and Eucalyptus, Followed by lyophilization, or a combination in which the powder is mixed with an extraction solvent.

The fermentation extract of Yarrow, Borage, Everlasting, Ladies' Mantle and Eucalyptus is not limited to a specific range, but is preferably contained in an amount of 0.0001 to 30% by weight based on the total weight of the cosmetic composition. When the content of the extract is less than 0.0001% by weight, the effect of improving the skin is not exhibited. When the content of the extract is more than 30% by weight, the effect of increasing the content of the extract is insignificant and there is a problem in safety and stability.

The ingredients contained in the cosmetic composition of the present invention include, in addition to the above-mentioned effective ingredients, the ingredients conventionally used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, And a carrier.

The cosmetic composition of the present invention may be prepared in any form conventionally produced in the art and may be in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, Oils, powder foundations, emulsion foundations, wax foundations and sprays, but are not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

The cosmetic composition of the present invention can be applied to a cosmetic for improving skin elasticity and inhibiting inflammation.

The cosmetic process of the present invention refers to all cosmetic processes using the cosmetic composition of the present invention. That is, all methods known in the art using a cosmetic composition belong to the cosmetic method of the present invention.

Further, the present invention provides a makeup method capable of obtaining a skin elasticity-improving skin effect and an inflammation reaction-inhibiting effect by using a cosmetic composition comprising the Yarrow, Borage, Everlasting, Ladies' Mantle and Eucalyptus fermented extract.

The cosmetic process of the present invention refers to all the cosmetic processes for applying the cosmetic composition of the present invention to human skin. That is, all methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. Further, the cosmetic composition having excellent blood circulation improving effect and / or skin elasticity improving effect according to the present invention can be used according to a conventional method of using, and the number of times of use can be changed according to a user's skin condition or taste.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention, and it is to be understood by those skilled in the art that the present invention is not limited thereto It will be obvious.

Example 1 Manufacture of Yarrow, Borage, Everlasting, Lady's Mantle, and Eucalyptus Fermented Extract

20 g of Yarrow, 20 g of Borage, 20 g of Everlasting, 20 g of Lady's mantle, and 20 g of Eucalyptus were selected as samples, thoroughly washed and dried, and finely pulverized so as to pass through a 300-mesh sieve. The culture medium of Saccharomyces sp. (Saccharomyces cerevisiae KCTC 7904) was added at a level of 0.5% based on the solid content of the pulverized product. (W / V) is added thereto, and 0.1 to 1% of peptone is added, preferably 0.5% (W / V) is further added thereto as a carbon source and 1 to 4% Respectively. The culture was incubated for 1 to 7 days, preferably 5 days, while maintaining the pH at 5-7, preferably at pH 6, using a 5 L fermenter at 37 ° C. After culturing the microorganism, the culture was centrifuged and the culture was firstly removed. Barrow, Everlasting, Ladies Mantle, and Eucalyptus whose cultures were firstly removed were heated to 50 캜, subjected to ultrahigh pressure treatment for 24 hours, and then cooled to room temperature. Ethanol was added to the final fermented yeow, barley, everlasting, ladys mantle and eucalyptus fermented product to make an aqueous 70% (v / v) ethanol solution. After refluxing for 3 hours for 5 hours, ) # 2 filter paper. When the filtration is completed, the extract obtained in the extracting step is transferred to a concentration tank and concentrated under reduced pressure at 50 DEG C or below, followed by lyophilization. The lyophilized product was dissolved in 2% of 50% glycerin to prepare 20 g (Example 1) of Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus fermented extract.

Example 2 Preparation of Nourishing Cream Containing Yarrow, Borage, Everlasting, Lady's Mantle, and Eucalyptus Fermented Extract According to the Present Invention

A step of heating the purified water portion to 80 DEG C to dissolve, a step of emulsifying by adding an oily portion heated and dissolved at 80 DEG C, a step of adding and mixing a thickener dissolved by heating at 60 DEG C, and a step of adding and mixing cosmetic ingredients And cooled to 30 ° C., degassed and filtered, and aged at 50 ° C. to produce nutritional cream as shown in Table 1.

Classification Raw material name Weight (% by weight) Purified water portion Purified water 60.36 Propylene glycol 5.0 glycerin 6.0 Xanthan gum 0.1 Triethanolamine 0.7 Methylparaben 0.2 Phenoxyethanol 0.1 The fermented extract of the present invention 2.0 Oil component Squalane 8.0 Cetyl alcohol 2.2 Cyclopentasiloxane 2.0 Stearic acid 1.5 Glyceryl monostearate 1.0 Microcrystalline wax 0.6 PIG-100 0.3 Tocopheryl acetate 1.0 Shi Butter 1.0 Propylparaben 0.1 Orizanol 0.1 Non-sabo roll 0.2 Dimethicone 0.5 Polyoxyethylene sorbitan monostearate 0.8 Thickener Carbomer 0.24 Purified water 2.4 Beauty ingredient Sodium ascorbate 0.1 Phospholipids 1.0 Prascenta extract 0.5 Aloe extract 0.5 Tocopherol acetate 0.5 Aloe extract 1.0 Sum 100.00

<Experimental Example 1> Skin irritation and protective effect of nutritional cream containing Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus fermented extract according to the present invention

A predetermined amount (10 m / 5 cm ² ) of the nutritional cream prepared in Example 3 was applied to the forehead portions of 30 healthy male and female (15 males and 15 females), and the other portions were covered with aluminum foil for 30 minutes Thereafter, a long wavelength ultraviolet ray was irradiated with a dose of 10.2 J / cm &lt; 2 &gt; using an ultraviolet ray irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, it was confirmed that the nutritional cream had no phototoxicity and had a skin protection effect because no adverse effects such as erythema were generated in the skin. In addition, 60 healthy adults (30 males and 30 females) were divided into two groups, and the experimental group in which the nutritional cream prepared in Example 2 was applied by a predetermined amount (10 m / 5 cm ² ) Example 2 was prepared without containing Ehrlesting, Lady's mantle and Eucalyptus fermented extract, and skin elasticity after 1 hour, 3 hours and 6 hours as a control group. The results showed that the disappearance rate of the experimental group was about 17% for each hour compared to the control group. As a result, the nutritional cream containing the Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus fermented extract of the present invention It was confirmed that the skin elasticity was improved by use.

<Experimental Example 2> Test for confirming the skin safety of nutritional cream containing Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus fermented extract according to the present invention

The nutrition cream prepared in Example 2 above was applied to the forehead of 30 healthy adult males (15 males and 15 females). The entire portion of the test subject was thoroughly wiped with 70% ethanol, and then the pin chamber containing 20 占 퐇 of the nutritional cream prepared in Example 2 was closed on the common portion for 24 hours by using an adhesive tape. After 24 hours, the adhesive tape and the pin chamber were removed, and the sample remaining on the skin with the gauze was gently wiped off and the skin condition was observed and again after 24 hours. As a result of the test, as shown in Table 2, no positive reaction was observed in the human skin test to confirm the skin irritation of the nutritional cream containing Yarrow, Borage, Everlasting, Ladies' mantle and Eucalyptus fermented extract according to the present invention, Was found to have no irritation.

sample After 24 hours (n = 30) After 48 hours (n = 30) Remarks Nourishing cream 0/30 0/30 Number of reaction proton / Total number of subjects

<Experimental Example 3> Measurement of antioxidative effect according to the concentration of fermented extract of Yarrow, Borage, Everlasting, Ladies mantle and Eucalyptus according to the present invention

Samples of control and experimental groups of Yarrow, Borage, Everlasting, Ladies Mantle, and Eucalyptus fermented extract were used in the same manner as in Experimental Example 3. Energy production in cells involves the production of reactive oxygen species (ROS), a byproduct of mitochondrial respiration, which causes damage to proteins, fats and nucleic acids. In order to protect the cell itself from such damage, the defense system works in the cell. However, when the balance between the production of free radicals and the antioxidant system is broken, oxidative stress acts on the cell, which is a major cause of various diseases and cancers of the body including aging. do. Therefore, the antioxidant ability of the extract is evaluated by measuring the amount of active oxygen formed in the cells for developing an antioxidant using a skin-friendly and stable plant as a raw material. DCFH-DA is a nonpolar molecule that can easily enter the cell and is separated by the intracellular esterase into DCFH, which is a nonpolar polar molecule. The DCFH-DA is a typical evaluation method using a fluorimetric assay using 2 ', 7`-dichlorofluorescin-diacetate. After oxidation by reactive oxygen species in the cells, they are converted to fluorescent DCF (2`, 7`-dichlorofluorescin), which is measured by a fluorometer. In FIG. 1, it was confirmed that the antioxidative effect was increased according to the concentration of the fermented extract of Yarrow, Borage, Everlasting, Lady's mantle, and Eucalyptus according to the present invention.

<Experimental Example 4> Measurement of inflammatory response inhibitory effect according to the concentration of the fermented extract of Yarrow, Borage, Everlasting, Ladis mantle and Eucalyptus according to the present invention

Samples of control and experimental groups of Yarrow, Borage, Everlasting, Ladies Mantle, and Eucalyptus fermented extract were used in the same manner as in Experimental Example 3. Nitric oxide (NO) is known to play a role in blood pressure regulation, neurotransmission, platelet aggregation inhibition, and immune function. It is synthesized by nitric oxide synthase from L-arginine in various tissues and cells. NOS is largely divided into cNOS and iNOS, and iNOS is independent of intracellular calcium concentration, and LPS, IFN-r, IL-1 and TNF in various cells such as macrophages, vascular smooth muscle cells, endothelial cells, hepatocytes and myocardial cells -a &lt; / RTI &gt; to produce a large amount of NO over a long period of time. However, when NO is generated more than necessary, it causes vasodilation due to shock, tissue damage caused by inflammatory reaction, damage of nerve tissue, and so on, and is harmful to living body. In the macrophage, NO produced by L-arginine eliminates tumor cells and fungi, and thus acts as an important substance in the function of macrophages involved in the immune response. In addition, NO acts as a major vasodilator. In this case, acetylcholine or bradykinin is used as a receptor. At this time, NO diffused to the surrounding smooth muscle cells increases cGMP and eventually relaxes the muscle cells by the increased cGMP and causes vasodilation. Based on this fact, development of an inflammatory drug that inhibits the production of NO by iNOS is under way, and the inhibitory effect of inflammation can be judged by measuring the NO production ability of the extract.

As an experimental method, RAW 264.7 cells, which are mouse macrophage cells, were treated with artificially induced inflammation-inducing substance LPS to evaluate whether they inhibited inflammation. Cells were seeded at 2 × 10 ⁵ cells per well in a 96-well plate and cultured in a 5% CO2 incubator at 37 ° C for 24 hours. Overnight, and then exchanged for new medium. LPS (1 ug / ml) and extract were added at different concentrations and cultured for 24 hours. After the culture, the supernatant of the medium was taken and centrifuged at 13,000 rpm for 3 minutes to collect the supernatant. The supernatant was collected and reacted with the Griess reagent reagent 1: 1 and the absorbance at 570 nm was measured. In FIG. 2, Nitric Oxide inhibitory effect was shown according to the concentrations of Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus extract.

<Experimental Example 5> Measurement of inhibitory effect on melamine synthesis in cells according to the concentration of fermented extract of Yarrow, Borage, Everlasting, Ladis mantle and Eucalyptus

Melanocytes are the differentiated cells in the basal layer of the skin epithelium, which have a special organs called melanocytes that synthesize melanin. Melanin is known to play an important role not only in protecting skin from ultraviolet rays, but also as a scavenger of cytotoxic radical, as well as pigmentation such as stains and freckles. At present, in order to evaluate the efficacy of whitening raw materials, in vitro tyrosinase activity inhibition test is most commonly used, and intracellular melanin formation inhibition test is most commonly used. This experiment evaluated the efficacy of the extract as a whitening ingredient through the inhibition of melanin synthesis in B16-F1 cells. In FIG. 3, melamine synthesis inhibitory effect was shown by the concentrations of Yarrow, Borage, Everlasting, Lady's mantle and Eucalyptus extract.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but on the contrary, &Lt; RTI ID = 0.0 &gt; and / or &lt; / RTI &gt;

Claims (7)

The method for producing a fermented extract for cosmetics, which comprises mixing 1 to 30 parts by weight of Yarrow, Borage, Everlasting, Ladis mantle and Eucalyptus, respectively, on a dry weight basis, fermenting yeast strains mainly and then centrifuging, A fermented extract for cosmetics produced according to the method of claim 1 An antioxidant functional cosmetic composition characterized by containing the fermented extract of claim 2 as an active ingredient An anti-inflammatory functional cosmetic composition characterized by containing the fermented extract of claim 2 as an active ingredient A skin elasticity-improving functional cosmetic composition characterized by containing the fermented extract of claim 2 as an active ingredient The cosmetic composition according to any one of claims 3 to 5, wherein the cosmetic composition is selected from the group consisting of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, By weight of the cosmetic composition The cosmetic composition according to any one of claims 3 to 5, wherein the cosmetic composition contains an auxiliary agent or carrier

Images