KR20160015718A - Feminine cleanser composition comprising Portulaca oleracea extract and Phytoncide - Google Patents

Abstract

The present invention relates to a female cleanser composition containing a glycyrrhiza extract and phytoncide, and more particularly, to a natural female cleanser composition containing a glycyrrhiza extract and phytoncide as an active ingredient and a method for producing the same. The composition of the present invention is a natural extract extracted from nature and can be applied without any side effects and toxicity in the application of the human body, has excellent antibacterial and antifungal effects, and is excellent in formulation excellence that can be prepared in various formulations, It is possible to commercialize it according to taste and convenience of the user.

Description

Feminine cleanser composition comprising Portulaca oleracea extract and Phytoncide,

The present invention relates to a natural female cleanser and a method for producing the same, and more particularly, to a natural female cleanser having excellent antimicrobial activity and containing a carnauba extract and phytoncide as an active ingredient, and a method for producing the same.

The quality of the woman is the connection between the vulva and the uterus, which is the most humid and warm place in the female body. Therefore, bacteria and fungi, which are the main causes of vaginitis, are suitable for habitat, so the diseases are likely to occur. Under normal conditions, many lactic acid bacteria live in the vagina, making the inside of the vagina mildly acidic and inhibiting the growth of pathogens and molds. However, it is difficult to prevent the growth of pathogens or fungi by using long-term antibiotics, long-term use of pills, use of tampons or loops or pregnancy or childbirth, When the balance inside the vagina breaks down due to hormonal balance changes, vaginitis may occur due to the proliferation of opportunistic pathogens that normally inhibit growth under normal conditions in the vagina. Vaginitis has been reported as bacterial vaginosis, Candida vaginitis, Trichomonas vaginitis and Atrophic vaginitis. The most common disease is bacterial vaginosis, caused by Gardnerella vaginalis. Candida albicans, common with bacterial vaginosis, is caused by the fungus Candida albicans.

In order to prevent such female diseases, a cleaning agent (vaginal cleanser) and a vulva cleanser are often used. Local cleaning agents commonly used to prevent vaginitis include transparent or rosin-type detergents which are obtained by sulfonating fatty acids or distilled lauryl alcohol and synthesizing emollients, thickeners, nutrients, foaming agents and organic disinfectants Have been shipped. In addition, there are surfactants with detergency and povidone-iodine as a medicine as a main component. It is known that povidone-iodine, which is a chemical disinfectant by chemical synthesis, is effective for the prevention and treatment of candidiasis vaginitis, trichomoniasis vaginitis and other vaginal infections. However, it is known that povidone- , Contact dermatitis, etc., and it is known that iodine or total iodine in blood may be elevated in the vagina to cause hypothyroidism. As a conventional technique for solving the side effects caused by the use of the disinfectant as a medicine as described above, the vaginal acidity is maintained at pH 3.2 by the secretion of lactic acid by vaginally beneficial microorganisms in healthy women, On the basis of the fact that the harmful bacteria are inhibited or killed, a buffer solution having pH 3.5, in which lactoserum extracted from milk and containing lactic acid as a main component is added, is proposed. However, It is difficult to expect fundamental treatment and prevention. In addition, polyene-based drugs include nyststin and amphotericinB, and azole-based drugs include imidazole-based drugs such as ketoconazole and miconazole, and triazole-based drugs such as itraconazole and fluconazole.

When such an antibiotic is administered in an insufficient amount, the germs do not die, resulting in a greater difficulty in the subsequent treatment because of resistance. In addition, the chemical quality detergent used as a detergent has a limitation in that the detergent is deteriorated in long-term use, fungi are removed instead of removing germs, and odor and unpleasant odor removing effect are inhibited. Even if many methods as described above are developed, there is a problem that recurrence occurs well.

On the other hand, in order to complement deficiencies of the vaginitis prevention and treatment agent, a composition for applying a sanitary napkin (Korean Patent Laid-Open Publication No. 2001-0014909) and a sanitary pad (Korean Patent Publication 2002-0062474) (Korean Patent Application No. 1979-0001672) discloses a cleaning composition for women (Korean Patent Application No. 10-2004-0072503), which is easy to clean and remove at mucous membrane sites in connection with a composition for local cleansing of women, (Korean Patent Application No. 10-20040085458), a composition of a quality detergent containing ginseng extract and a method for producing the same (Korean Patent Application No. 10-2004-0085204), prevention and treatment of vaginitis A method of extracting an effective ingredient useful for treatment and a detergent composition containing the extract extracted therefrom (Korean Patent Laid-Open No. 10-20 (Korean Patent Application No. 10-1999-0037078), a herbal composition for local cleansing of women including gosams, lobes, peonies and red ginseng (Korean Patent Application No. 2003-004587), a cleaner composition for women containing propolis 10-2009-0023172). As described above, various drugs have been proposed as a method for treating vaginal cleansing and abnormal symptoms, but there is still a lack of presentation of non-irritating drugs considering that the site of use is the most sensitive mucosal site for foreign substances. Thus, there is a need to develop new technologies to meet the continuous need for compositions using natural extracts that are free of irritation and side effects.

In order to solve the above problems, the present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a pharmaceutical composition for preventing and treating symptoms by preventing human adverse effects and irritation, In addition, when applied to a cleanser composition for women, there is no deterioration in the convenience of the formulation and application thereof, so that various formulations can be manufactured and manufactured so that they can be used appropriately. ≪ / RTI >

It is an object of the present invention to provide a female detergent composition having antimicrobial and antifungal activity, which contains an extract of Aspergillus oryzae and phytoncide extracted from a cottonwood as an active ingredient as a natural extract having low side effects and toxicity.

In order to attain the above object, the present invention provides a female cleanser composition comprising a glycan extract and phytoncide as an active ingredient.

In addition,

1) washing the leggings, drying the leggings, extracting them with water, an alcohol or an aqueous alcohol solution, and then filtering them to prepare a glycyrrhiza extract;

2) neutralizing the mixture of legume extract and phytoncide in step 1); And

3) mixing the neutralized mixture of step 2) with purified water.

In addition, the present invention provides a pharmaceutical composition for antibacterial and antifungal use, which contains a carnauba extract and phytoncide as an active ingredient.

In addition, the present invention provides a quasi-drug composition for antimicrobial and antifungal use, which contains a carnauba wax extract and phytoncide as an active ingredient.

In contrast to the prior art, the present invention is based on the discovery that conventional edible vaginitis medicines may cause liver toxicity, digestive disorders, resistance to vaginitis causing bacteria, and in case of applied ointment, contact dermatitis occurs due to skin irritation However, the composition of phytolitic extract and the phytoncide extracted from cottonwood according to the present invention is a natural product and can be applied without risk because it has no adverse effects such as irritation to the mucous membrane of the skin in the application of human body and toxicity, and has excellent antimicrobial activity , It is possible to remarkably alleviate the lesions of Candida albicans and bacterial vaginitis, and it is possible to manufacture various types of formulations because of the convenience of formulation and the deterioration of effect upon application.

Hereinafter, the present invention will be described in detail.

The present invention provides a female detergent composition comprising a glycan extract and phytoncide as an active ingredient.

Here, leggings are one year old weeds of the genus Pseudomonas, and they are also called as the five legs, the long legs, and the horses, and they grow naturally on the roads and in the garden, There are no hairs in the range of 15 ~ 30cm, the stem is reddish, the branches are split a lot and spread diagonally over the ground. The roots are white, but when scratched by hand, they turn red as the main stem. Leaves are opposite to each other or alternate, but those at the end of the leaves are annihilated. The leaf form is egg-shaped, the end is round, the bottom is narrowed, and it becomes short petiole. Leaf length is 1.5 to 2.5 cm, width is 0.5 to 1.5 cm, and the edge is plain. Flowers bloom around June to October, and the flowers are yellow. They are positive and hang on the ends of the branches. There are two calyxes and ovals. There are five petals, and the flowers bloom in the middle of the day and soon it is called Oh. There are seven to twelve stamens and one pistil, and the ovaries are located in the central region, with five styles. The dogs are oval in August, but the canines are oval, and the central part is divided into sides, resulting in a large number of seeds with long stalks. These leggings are used as seasonings and edible or as medicines.

Here, phytoncide is a substance extracted from cottonwood. Chamaecyparis obtusa, also known as cypress tree, is an evergreen tree of the ginseng plant, a plant species, and is widely cultivated as a planting species in the southern part of Korea through development, although it is originated in Japan. , Antibacterial agents and the like. Phytoncide is a self-defense substance of plants. It is a substance that plants excrete or secretes to resist pathogens, insects and fungi, and contains sterilization and insecticides. The constituent material of phytoncide is an organic compound composed of a phenol compound such as terpene, an alkaloid component, a glycoside, etc., and is known to have antimicrobial action, sedative action, deodorizing action and stress relieving action. Such phytoncide is a natural substance, not a chemical synthetic substance, and selectively absorbs bacteria which are absorbed in human body and harmful to human being. Phytoncide is known to have many functions such as antimicrobial action, deodorizing action, sedative action and stress relieving action. In addition, phytoncide has excellent sterilization, antibacterial and cleansing action, cleansing the skin, moisturizing and enhancing the immune function in the body.

In the composition according to the present invention, the glycyrrhiza extract and phytoncide are preferably mixed at a ratio of 1: 4: 1 to 4: 1, more preferably 1: 1.

The glycyrrhiza extract is preferably an extract obtained by extracting legumes as a solvent with water, alcohol or a mixture thereof. The extraction temperature may be 5 to 80 ° C., more preferably 5 to 25 ° C., and the extraction time may be 50 to 100 ° C., 200 days, and more preferably 100 to 150 days. The extract may be filtered, concentrated or dried to remove the solvent. Filtration, concentration and drying may all be performed. The filtration may be performed using a filter paper or a vacuum filter, The concentration can be reduced by using a reduced pressure concentrator, for example, a rotary evaporator, and the drying can be performed, for example, by the freeze drying method.

In the composition according to the present invention, it is preferable that the composition further comprises at least one selected from the group consisting of citron extract, rosemary extract, caspase extract, and leaf extract. In the case where the composition of the present invention further contains the extract of Chenopodium acuminata, the extract of Chenopodium ambrosioides, the extract of caspase-3, and the extract of Quercus acutissima, the extract of Pseudomonas sativus 1 to 4: Phytoncide 1 to 4, Astragalus 1 to 4, Chenopodium 1 to 4, Caspase 1 to 4, It is preferable to mix them at a ratio of 1 to 4, and it is more preferable that they are mixed at the same ratio.

Here, the scientific name of Cnidium officinale MAKINO is 30 ~ 60 ㎝ in height and grows straight and branches are divided. Leaves are alternate phyllotaxis with two alternate phyllotaxis. Leaves are long and petiole is long. Leaves are gradually smaller as they go up. Leaves are leaf sheath and wrap around main stem. Leaves are ovate or lanceolate. There are sharp cogs along with cogs. Flowers bloom in August, and large phloem episodes develop at the ends of the branches and the main stem. The young seeds eat as herbs and the rootstock is dried and used as medicinal materials. The ingredient contains a large amount of aromatic essential oil, so it has a good smell. In pharmacological experiments with animals, it was found to have a sedative effect on the central nervous system, and a weak but sustained hypotensive effect was also confirmed. In the experiment on smooth muscle, the contraction power of the uterus was increased, and the sterilization was also effective in suppressing the development of Escherichia coli, Dysentery, Pseudomonas aeruginosa, and Skin fungi.

Here, Salvia Miltiorrhiza Root is the root of Dansam (Salvia miltiorrhiza Bunge (Lamiaceae and Labiatae)), which has a short rhizome and rough edges, and usually has stalks on the top. The water extract of Dansam has an inhibitory effect on various skin fungi and it is used for the treatment of bronchial hyperplasia, menopause, postpartum embarrassment, rheumatism with antibiotics, bronchodilator, pain medication, antiinflammatory drug, . It is mainly treated with gynecological diseases, but it has the same therapeutic effect as the soup even if it takes out only the root.

Here, Cnidi Fructus is the fault of Cnidium monieri (L. Cuss) or Casualties (Torilis japonica Decandolle). It is said that the genital hernia of the female is swollen and sick, the genital area is treated to treat humid and itchy symptoms, and it is effective for the vaginal cannibalism, skin pruritus, and females.

Here, Kochiae fructus is a mature fruit of the Kochia scoparia Schrader. Branch owners control gonorrhea and wives and their skin and are also applied to skin diseases caused by moist heat, such as pruritus and eczema.

The composition according to the present invention is not particularly limited in its use, and can be used, for example, in a female detergent composition for prevention or treatment of vaginitis. In addition, the above-described cleaner composition for women can be used for a detergent, a medicine, a cosmetic, a wet tissue, or the like.

The detergent may be in the form of a spray, a liquid, a gel, a powder, a solid soap, a foam, and the like. The cosmetic may optionally include a lotion, lotion, cream, The pharmaceutical preparation may be a solid preparation for oral administration, which is formulated into tablets, pills, powders, granules, capsules and the like, and may be parenterally administered A cream, an ointment, a lotion, a dressing solution, a spray, and other coating agents.

The composition according to the present invention is preferably contained in an amount of 10% by weight to 100% by weight based on the total weight of the female cleaner composition when it is used in a female cleaner composition.

In the present invention, in addition to the composition according to the present invention, in addition to the composition according to the present invention, a female cleansing composition for a cosmetic or external preparation for skin may contain various components generally usable in external preparations for skin such as a water- , Oil, surfactant, moisturizing agent, viscosity controlling agent, preservative, antioxidant, fragrance, pigment and the like. The female cleansing composition for cosmetic or dermatological use may further comprise at least one additive selected from the group consisting of oil, water, surfactant, moisturizing agent, thickening agent, chelating agent, coloring agent, preservative and fragrance .

In addition,

1) washing the leggings, drying the leggings, extracting them with water, an alcohol or an aqueous alcohol solution, and then filtering them to prepare a glycyrrhiza extract;

2) neutralizing the mixture of legume extract and phytoncide in step 1); And

3) mixing the neutralized mixture of step 2) with purified water.

In the above production process, it is preferable that the drying of step 1) is carried out at 5 to 25 ° C for 20 to 40 hours to evaporate moisture, and more preferably to evaporate moisture at 5 to 15 ° C for 24 to 30 hours Do.

In the above production method, the extraction of step 1) is preferably carried out by mixing the dried glycyrrhiza with water, an alcohol or an aqueous alcohol solution, and then extracting the mixture at 5 to 25 ° C for 50 to 200 days, It is more preferable to immerse in 100 to 150 days for extraction.

In the above preparation method, the mixture of step 2) is preferably mixed with carnauba wax extract and phytoncide at a ratio of 1: 4: 1 to 4: 1, and more preferably 1: 1.

In the above-mentioned preparation method, at least one selected from the group consisting of cucurbitaceae extract, ginsenosides extract, caspase extract, and leaf extract can be further mixed in step 2). It is preferable that the mixing ratio is 1 to 4 weight ratio.

In the above production method, the purified water in step 3) is preferably 70 to 80 ° C.

In addition, the present invention provides a pharmaceutical composition for antibacterial and antifungal use, which contains a carnauba wax extract and phytoncide as an active ingredient.

In addition, the present invention provides a quasi-drug composition for antibacterial and antifungal use, which contains a carnauba wax extract and phytoncide as an active ingredient.

In the antimicrobial and antifungal composition according to the present invention, it is preferable that the composition further comprises at least one selected from the group consisting of citron extract, ginseng extract, caspase extract,

In the present invention, the composition for antibacterial and antifungal use according to the present invention is a composition comprising 10% to 100% by weight of a mixture of Peppermint Extract and Phytoncide according to the total weight of the composition, wherein Candida albicans (Candida albicans) and a general bacterium (Gardnerella vaginalis).

In the present invention, the composition for antibacterial and antifungal use may be used as a cosmetic, a skin cleanser, a disinfectant, a wet tissue or the like containing antibacterial and antifungal functions.

Hereinafter, the present invention will be described in more detail by way of Examples, Experimental Examples and Preparation Examples. It is to be understood that both the present invention and the following examples are for illustrative purposes only and that the scope of the present invention is not limited by these examples, And will be apparent to those skilled in the art.

< Example  1 > Preparation of a composition containing glycyrrhiza extract and phytoncide

After picking the leggings, they were cleaned and then exposed to low temperature (10 ° C) for 24 hours to evaporate water. Then, dried pepper and alcohol were mixed and allowed to stand at low temperature (10 ° C) for about 100 days and then filtered using a filter paper. Then, 100 grams of phytoncide extracted from cottonwood and pepper extract were neutralized by mixing. Then, it was mixed with purified water heated to 70 to 80 ° C and activated once more to make it into a heavy water state.

< Experimental Example  1> Antimicrobial activity measurement using disk diffusion method

In order to confirm the antibacterial activity of the composition prepared in Example 1, the disc diffusion method was used.

<1-1> Candida albicans  Evaluation of antibacterial activity against

100 μl of pre-cultured Candida albicans (Sabouraud Dextrose Agar.Difco.USA) was plated on a disk medium for determination of disk diffusion method, and the extract was applied to a sterilized paper disk at a concentration of 5 mg / disc After the sample was dropped, it was placed on the medium. As a control, itraconazole, an antifungal agent, was placed on a paper disk at a concentration of 100 μg / disk. After the plate medium was incubated at 28 ° C for 48 hours, the size of the growth zone (Clean Zone) was measured. The results are shown in Table 1 below. As shown in the following Table 1, the composition of the present invention showed strong growth inhibition effect compared to itraconazole, which is a control group.

<1-2> Guard nerella In pants naris  Evaluation of antibacterial activity against

100 μl of pre-cultured Gardnerella vaginalis was dispensed into a Reinforced clostridial medium (Difco.USA) for determination of disk diffusion method, and the extract was applied to a sterilized paper disk at 5 mg / disc And then placed on the culture medium. As a control, Ampicillin, an antibiotic, was placed on a paper disk at a concentration of 10 μg / disk. After culturing the plate medium at 37 ° C for 48 hours, the size of the growth zone (Clean Zone) was measured. The results are shown in Table 1 below. As shown in Table 1, the antimicrobial composition of the present invention showed a strong growth inhibition effect.

Strain The composition of Example 1 Control group Candida albicans 25 30 Guard Nella Trousers 27 32

< Experimental Example  2> Minimum inhibitory concentration using liquid medium susceptibility test MIC )

In order to confirm the antimicrobial activity of the composition prepared in Example 1, the antimicrobial activity and IC ₅₀ (half maximal inhibitory concentration) required for 50% inhibition of microorganisms were measured using a Broth Microdilution Susceptibility Test ) Were measured.

<2-1> Candida Albikanse  Evaluation of antibacterial activity against

The cell number of Candida albicans was diluted with Sabouraud Dextrose medium to 2 × 10 ⁵ cells / ml and treated with serial dilution method in 96-well. After incubation at 28 ° C for 24 hours, the absorbance was measured at a wavelength of 570 nm using a microplate reader (BIO-RAD) to determine IC ₅₀ values. The results are shown in Table 2 below.

<2-2> Guard nerella In pants naris  Evaluation of antibacterial activity against

The cells were diluted with Sabouraud Dextrose medium to a cell density of 2 × 10 5 cells / ml and treated with serial dilution in 96-wells. After incubation at 37 ° C for 24 hours, the absorbance was measured at a wavelength of 570 nm using a microplate reader (BIO-RAD) to determine IC ₅₀ values. The results are shown in Table 2 below.

Strain IC ₅₀ (mg / ml) value Candida albicans 28.5 Guard Nella Trousers 20.4

< Experimental Example  3> Measurement of effect on microorganisms

Tests were conducted to examine the effect of the composition prepared in Example 1 on Lactobacillus acidophilus, Candida albicans and Gardnerella vaginalis. Respectively.

The same procedure as in <Experiment 2> was conducted. The present method can confirm whether the microorganism is proliferated or not through the increase of the turbidity in the medium. Thus, the survival rate of the microorganism to the composition of the present invention can be obtained. The results are shown in Table 3. As a result, it was found that the composition of the present invention had no significant effect on Lactobacillus ashidophilus but inhibited the growth of Candida albicans and Gardnerella vaginalis.

Concentration (mg / ml) 5 10 50 100 Survival rate of Lactobacillus acidophilus (%) 100 100 89.5 85.0 Survival rate of Candida albicans (%) 45.2 15.4 2.2 0 Survival rate of Gardnerella vaginalis (%) 65.5 19.5 4.8 0

< Manufacturing example  1> For women in aerosol spray formulations Cleaner  Preparation of composition

Aerosol sprays were prepared by the following ingredients to prepare a female cleanser composition for the prevention or treatment of vaginitis, including the composition of the present invention, in an aerosol spray formulation.

<Ingredient Content (wt%)>

The composition of the present invention 20%

Surfactant 0.15%

Alkali metal benzoate 0.015%

Purified water balance

Total 100%

< Manufacturing example  2> Gel type  Formulations (cream) for women Cleaner  Preparation of composition

Creams were prepared with the following ingredients to prepare a female cleanser composition for the treatment and prevention of vaginitis, including the composition of the present invention.

<Ingredient Content (wt%)>

The composition of the present invention 20%

Dimethicone copolyol 10.0%

Cyclomethicone 10.0%

Urea 20.0%

Glycerin 20.0%

Triethanolamine 0.02%

Preservatives 1.0%

Purified water balance

Total 100%

< Manufacturing example  3> For women of liquid type formulation (wet tissue) Cleaner  Preparation of composition

A liquid composition was prepared by the following components to prepare a female cleanser composition for the treatment and prevention of vaginitis, including the composition of the present invention.

<Ingredient Content (wt%)>

The composition of the present invention 20%

Sodium lauryl sulfate 2.0%

Urea 1.0%

L-menthol 0.5%

Polyoxyethylene nonylphenyl ether 0.8%

Ethanol 15%

EDTA 0.05%

Preservatives 1%

Purified water balance

Total 100%

The above liquid composition was prepared and applied to a fabric (non-woven fabric) for a wet tissue.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, And falls within the scope of the present invention.

The composition according to the present invention can be commercialized into various formulations such as cosmetics, medicines, cleaning agents and wet tissues as a female cleanser.

Claims (15)

A female cleansing composition comprising pepper extract and phytoncide as an active ingredient.
The female detergent composition according to claim 1, wherein the sorghum extract and phytoncide are mixed in a weight ratio of 1: 4: 1 to 4: 1.
The female detergent composition according to claim 1, wherein the sorghum extract is extracted with water, alcohol, or a mixture thereof.
2. The method of claim 1, wherein the phytoncide is extracted from cottonwood.
The female detergent composition according to claim 1, further comprising at least one member selected from the group consisting of Astragalus sinensis extract, Panax ginseng extract, Caspase extract and Leaf extract.
6. The female detergent composition according to claim 5, wherein the female cleansing composition is mixed at a ratio of Peppermint Extract 1 to 4: Phytoncide 1 to 4, Peppermint 1 to 4, Peppermint 1 to 4, Casualties 1 to 4, and Pepper 1 to 4.
6. The composition of claim 1 or 5, wherein the composition is a detergent, a cosmetic, or a wet tissue formulation.
1) washing the leggings, drying the leggings, extracting them with water, an alcohol or an aqueous alcohol solution, and then filtering them to prepare a glycyrrhiza extract;
2) neutralizing the mixture of legume extract and phytoncide in step 1); And
3) mixing the neutralized mixture of step 2) with purified water.
The method for preparing a female detergent composition according to claim 8, wherein the drying of step 1) is performed at 5 to 25 ° C for 20 to 40 hours to evaporate moisture.
[9] The method according to claim 8, wherein the extraction of step 1) comprises mixing the dried gazebo with an aqueous solution of water, alcohol or alcohol, and then immersing the mixture at 5-25 ° C. for 50 days to 200 days .
[9] The method of claim 8, wherein the mixture of step 2) is mixed at a ratio of 1: 4: 1 to 4: 1 to 4: 1 to 4: 1.
The method for preparing a female detergent composition according to claim 8, wherein the step (2) further comprises mixing at least one selected from the group consisting of citron extract, ginsenosides extract, caspase extract, and extracts.
The method of claim 8, wherein the purified water in step 3) is 70 to 80 ° C.
The present invention relates to a pharmaceutical composition for antifungal and antifungal use, which contains a carnauba wax extract and phytoncide as an active ingredient.
An antimicrobial and antifungal composition comprising as an active ingredient a carnauba wax extract and phytoncide.