KR20240021066A - Composition for feminine hygiene containing natural oil as an active ingredient and method for manufacturing the same - Google Patents

Abstract

The present invention relates to a composition for feminine hygiene management containing natural oil extracted from Copaiba resin as an active ingredient. Specifically, it contains natural oil as an active ingredient, so it is safe and can be applied externally to prevent or treat female diseases. It relates to a composition that can be used as a feminine cleanser.

Description

Composition for feminine hygiene containing natural oil as an active ingredient and method for manufacturing the same}

The present invention relates to a composition for feminine hygiene management containing natural oil extracted from Copaiba resin as an active ingredient, and to a composition and method for producing the same that can be applied externally to prevent or treat female diseases or as a feminine cleanser. will be.

A woman's vagina is the connecting passage between the vulva and the uterus and is the moistest and warmest place in the woman's body. A woman's vagina is a suitable environment for bacteria and fungi, which are the main causes of vaginitis, to live, so there is a high possibility of developing diseases due to them. Additionally, the groin area, also known as the Y zone, is humid and warm, so if ventilation is lacking due to women's underwear or clothing that does not allow for comfortable movement, bacterial infection or inflammation is likely to occur.

Vaginitis is reported to include bacterial vaginosis, Candida vaginitis, Trichomonas vaginitis, and atrophic vaginitis. The most common disease is bacterial vaginitis, which is caused by Gardnerella vaginalis. It is caused by Gardnerella vaginalis, and candidal vaginitis, which is common along with bacterial vaginosis, is caused by the fungus Candida albicans.

A variety of antibiotics, fungicides, protozoans, and anti-inflammatory drugs have been proposed to treat female diseases such as vaginitis or groin inflammation, but there is a risk of resistance and there are limits to their free use because they are irritating.

Povidone, glycoester, and glycoflavonoid ingredients can be used as feminine cleansers, but these have the disadvantage of having a foul odor or being inconvenient to use because they must be washed with water after use.

Accordingly, the present inventors designed a composition for feminine hygiene management that contains natural ingredients and is safe and convenient to use.

One aspect provides a composition for feminine hygiene care, comprising copaiba extract and argan oil as active ingredients.

Another aspect provides a feminine cleanser comprising copaiba extract and argan oil as active ingredients.

Another aspect provides a composition for treating or preventing female diseases containing copaiba extract and argan oil as active ingredients.

One aspect provides a composition for feminine hygiene care, comprising copaiba extract and argan oil as active ingredients.

The term “for feminine hygiene management” means that it is for the treatment or prevention of female diseases that may occur due to feminine cleanser or neglect of feminine hygiene management. The female disease may mean vaginitis or groin inflammation.

The term “containing as an active ingredient” may mean containing an effective amount that can exhibit a feminine cleansing effect, for example, antibacterial, anti-inflammatory, itching, pain, etc.

Copaiba is extracted from the resin of the Copaiba tree and has inflammation-relieving, analgesic and skin-soothing effects. Copaiba extract contains more than 80 w/w% of cannabinoids, and the cannabinoids may specifically be beta-karyophyllen. The Copaiba extract may be an essential oil obtained by steam distillation.

Argan oil is an oil extracted from the seeds of the argan fruit. It contains a large amount of squalene and is excellent for removing toxins, moisturizing the skin, regenerating, and anti-inflammatory effects.

The copaiba extract and argan oil each have a certain degree of moisturizing, anti-inflammatory and antibacterial effects, but surprisingly, it was confirmed through antibacterial and anti-inflammatory tests that when they are mixed, significantly superior moisturizing, anti-inflammatory and antibacterial effects can be obtained.

In the composition, copaiba extract and argan oil may be included in a weight ratio of 1:1 to 2 as extracts with a purity of 90% or more, preferably 99% or more.

The composition includes evening primrose seeds, lavender, tea tree, clary sage, Frankincense, Commiphora Myrrha, Neroli, rose, Geranium, and Ylang Ylang as additional active ingredients. (Ylang Ylang), jasmine, jojoba, acacia flower, chamomile, vetiver, or a combination thereof may be further included.

The evening primrose extract is a seed oil obtained by extracting the oil component from the seeds of the evening primrose, and can exhibit anti-inflammatory effects in women's diseases as well as moisturizing and regenerating the skin.

The jojoba oil is an oil component extracted from the seeds of jojoba fruit, and can exhibit moisturizing and anti-inflammatory effects.

The lavender, clary sage, neroli, rose, geranium, ylang-ylang, jasmine, acacia flowers, and chamomile may be essential oils extracted from flowers by steam distillation, or may be floral water obtained by separating only water-soluble substances from the essential oils.

The tea tree is an extract of leaves and stems, and may be an essential oil obtained by steam distillation.

The vetiver is an extract of the root and may be an essential oil obtained by steam distillation.

The frankincense is a resin extract of the Boswellia Carteri tree and may be an essential oil obtained by steam distillation.

The myrrh is a resin extract of Commiphora Myrrha and may be an extracted oil or an essential oil obtained by steam distillation.

The additional active ingredient has a skin soothing and inflammatory effect and can further enhance the moisturizing, antibacterial, and anti-inflammatory effects of copaiba and argan oil.

In one embodiment, the composition may include argan oil, jojoba oil, evening primrose oil, copaiba essential oil, and lavender essential oil.

When the composition for feminine hygiene care includes additional active ingredients, 6 parts by weight of a mixture of argan oil, jojoba oil, and evening primrose oil; and 4 parts by weight of a mixture of copaiba essential oil, and lavender essential oil. Here, argan oil, jojoba oil, and evening primrose oil may be included in 1:1:1 parts by weight, and copaiba essential oil and lavender essential oil may be included in 1:1 parts by weight. The ratio of each active ingredient may be important not only for efficacy, but also for feeling of use, appropriate volatility, and formulation.

In the composition for feminine hygiene care, each natural product is included as an extract. The term “extract” refers to a solvent into which the active ingredient contained in the extraction raw material is transferred by contacting the solvent and the extraction raw material under specific conditions. If the substance is separated from a natural product, the extraction method or type of ingredient All can be included regardless. For example, it may include those obtained by extracting solvent-soluble components from natural products using water or an organic solvent, and those obtained by extracting only specific components such as oils from natural products. The extract may be extracted with one or more solvents selected from the group consisting of purified water, lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-buren glycol, and the alcohol has 60 to 90 carbon atoms. It may be ethanol at a % concentration (v/v). When extracting the natural product as an essential oil, it can be extracted using commonly known methods such as distillation, compression, enplage, soaking, and solvent extraction. The natural products may be specifically included in the composition as base oil, essential oil, or water extract.

The composition for feminine hygiene care is formulated with one or more selected from the group consisting of softening lotion, astringent lotion, nourishing lotion, lotion, nourishing cream, massage cream, essence, cleansing cream, cleansing foam, cleansing water, pack, spray, and powder. You can get angry.

In the composition for feminine hygiene care, the mixture of the extracts may be included in an amount of 5 parts by weight or more, preferably 5 to 30 parts by weight, and more preferably 8 to 12 parts by weight, based on 100 parts by weight of the total composition. If the mixture of the above extracts is less than 5 parts by weight in the total composition, the medicinal effect of the active ingredient may not be fully expressed, and if it exceeds 30 parts by weight, it may irritate the skin.

The composition for feminine hygiene management can be used as an external agent or sitz bath. The term “external use” refers to application directly to the affected skin, and the term “sitoon” refers to adding an appropriate amount to hot water, evaporating the active ingredient with water vapor, and applying it to the skin. The composition for feminine hygiene management may contain an appropriate solvent to facilitate absorption of the active ingredient when used as an external agent or situ, and to allow the drug to be properly volatilized and fermented to the application site and surrounding areas, and the solvent is propanediol. , butylene glycol, 1,2-hexanediol, ethylhexylglycerin, panthenol, purified water, or a combination thereof.

In each formulation, the composition for feminine hygiene care may be appropriately mixed with other ingredients in addition to the essential ingredients within the range that does not impair the purpose of the present invention, depending on the type of formulation or purpose of use.

Compositions for feminine hygiene care may contain commonly acceptable carriers, such as oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, colorants, preservatives, fragrances, etc., and may be appropriately mixed, but are limited thereto. It doesn't work.

The acceptable carrier may vary depending on the formulation. For example, when formulated into ointments, pastes, creams or gels, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or these are used as carrier ingredients. Mixtures may be used.

When the composition for feminine hygiene care is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silcate, polyamide powder, or a mixture thereof may be used as a carrier ingredient, and in the case of a spray, chlorochloride It may further include propellants such as fluorohydrocarbon, propane, butane or dimethyl ether.

When the composition for feminine hygiene care is formulated as a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil may be used, in particular cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan. You can.

When the composition for feminine hygiene care is formulated as a suspension, the carrier component includes water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester. Suspending agents, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the composition for feminine hygiene care is formulated as a soap, the carrier ingredients include alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionates, lanolin derivatives, fatty alcohols, vegetable oil, glycerol, Sugar, etc. may be used.

The composition for feminine hygiene care includes fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, and foaming agents commonly used in the industry, depending on the quality or function of the final product. ), fragrances, surfactants, water, ionic or non-ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic activators, common in cosmetics. It may additionally contain auxiliaries commonly used in the fields of cosmetology or dermatology, such as any other ingredients used.

However, the auxiliaries and their mixing ratio may be appropriately selected so as not to affect the desirable properties of the feminine cleanser composition according to the present invention.

The composition for feminine hygiene management according to one aspect is convenient to use because it uses natural ingredients that are safe for the skin as active ingredients, and has excellent antibacterial, anti-inflammatory, and moisturizing effects, so it effectively removes bacteria that cause vaginitis or groin inflammation. , It is effective in alleviating symptoms of vaginitis such as coldness, itchiness, and bad odor.

In addition, the composition for feminine hygiene management according to one aspect is convenient to use because it can be applied directly to the skin, and can be used in methods such as fumigation or situating, so it can be used in formulations such as vaginal tablets commonly prescribed for the treatment of female diseases such as vaginitis. Compared to others, there is less reluctance to use it.

Hereinafter, the present invention will be described in more detail based on the following examples, but these are only for illustrating the present invention and do not limit the scope of the present invention in any way.

Preparation Example 1: Preparation of composition for feminine hygiene care

Copaiba essential oil, argan seed oil, jojoba oil, evening primrose oil, and lavender essential oil were all 99.9% pure.

Mix 10 parts by weight of propanediol, 10 parts by weight of butylene glycol, 10 parts by weight of 1,2-hexanediol, 10 parts by weight of ethylhexylglycerin, 1 part by weight of panthenol, and 0.01 parts by weight of EDTA-Na ₂ and add purified water. A solvent mixture was prepared so that the total amount was 100 parts by weight. A total of 100 g of the example composition was prepared by mixing natural oil with the composition shown in Table 1 below into the solvent mixture prepared above.

(g) Example Comparative example One 2 One 2 3 4 5 6 7 Copaiba Resin 2 4 4 - 4 4 - - - lavender 2 - - 4 - - 4 4 4 argan seeds 2 6 - 2 - - 6 3 - jojoba seeds 2 - 3 2 6 - - - 3 evening primrose seeds 2 - 3 2 - 6 - 3 3

Test Example 1: Cell safety evaluation

To evaluate the safety of the composition of the present invention when applied to the skin, a cytotoxicity test was performed on human skin fibroblasts.

Specifically, HDF (Human Dermal Fibroblast) cells were dispensed into a 96-well plate at a concentration of 1 × 10 ⁴ cells/well, incubated at 37°C and 5% CO ₂ for 24 hours, and each of the cells prepared in Preparation Example 1 was added. Examples and comparative examples were treated with cells at concentrations of 100, 50, and 25 μg/μl and incubated again for 24 hours. Then, the medium was removed and treated with MTT{3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide} at a concentration of 5 mg/mL and incubated at 37°C and 5% CO ₂ for 2 hours. After incubation, 100 ㎕ of DMSO (Dimethyl sulfoxide) was added, stirred for 10 minutes, and absorbance was measured at 540 nm.

As a result of the measurement, no cytotoxicity was observed at all concentrations of all tested Examples and Comparative Examples.

Test Example 2: Evaluation of antibacterial activity

To evaluate the antibacterial activity against Candida albicans and Gardnerella vaginalis, the bacteria that cause vaginitis, one of the female diseases, disc diffusion method was performed.

Specifically, 100 ㎕ of Candida albicans and Gardnerella vaginalis were dispensed onto a plate medium for disc diffusion measurement (Sabouraud Dextrose Agar. Difco. USA) and plated. Then, the Example and Comparative Example compositions prepared in Preparation Example 1 were dropped onto a sterilized 8 mm paper disk at a concentration of 5 mg/disc and placed on the medium.

As positive controls, Itraconazol was used for Candida albicans and Ampicillin was used for Gardnerella vaginalis, each at a concentration of 100 ㎍/disk.

After each example and drug treatment, the plate medium was cultured at 28°C for 48 hours and the size (mm) of the growth-inhibiting zone (clean zone) was measured, and the results are shown in Table 2 below.

(mm) candida albicans Gardnerella vaginalis Positive control group 36 35 Example 1 31 32 Example 2 27 26 Comparative Example 1 19 19 Comparative Example 2 20 20 Comparative Example 3 19 21 Comparative Example 4 15 14 Comparative Example 5 13 16 Comparative Example 6 14 16 Comparative Example 7 10 11

As a result, it was confirmed that Examples 1 and 2 had excellent antibacterial activity by creating a growth-inhibiting ring that was significantly wider than that of Comparative Examples 1 to 7.

Test Example 3: Evaluation of anti-inflammatory effect

To evaluate the anti-inflammatory effect of the composition of the present invention, NO activity was measured.

Specifically, RAW264.7 cells were seeded in a 96-well microplate at a concentration of 1*10 ³ cells/well, then 10% (v/v) fetal bovine serum (FBS), 100 units/ml penicillin, 100 ㎍ /㎖ was incubated in DMEM medium containing streptomycin for 24 hours. Then, each well was treated with 0.01 μg/ml of LPS, Examples, and Comparative Example compositions at a concentration of 25 μg/μl, incubated for 24 hours, and then 50 μl of the supernatant was transferred to a new 96 well and NO (Nitric oxide) was added to each well. ) 50 ㎕ of Griess Reagent was treated, reacted for 15 minutes, absorbance was measured at 540 nm, NO activity (%) was calculated, and is shown in Table 3 below.

NO activity (%) Purified water 20.56 LPS 120.38 Example 1 38.23 Example 2 47.56 Comparative Example 1 90.32 Comparative Example 2 70.67 Comparative Example 3 88.95

As a result, it was confirmed that Examples 1 and 2 were significantly superior to Comparative Examples 1 to 3 in suppressing inflammation induced by LPS.

Test Example 4: Evaluation of treatment efficacy for female diseases

It was confirmed whether the composition of the present invention could be used externally to improve vaginitis or groin skin health.

Specifically, 30 women in their 20s to 40s were divided into groups of 5 and the compositions of Examples 1 and 2 and Comparative Example 2 were applied around the groin area or 10 to 20 drops per liter of water were applied. It was applied once a day in a sit-down manner, and a survey was conducted about the feeling of use after 2 weeks. Each item regarding feeling of use was evaluated as “Not at all (1), Not at all (2), Average (3), Agree (4), Very Agree (5),” and the average was calculated in Table 4 below. indicated.

question apply directly Left speech Example 1 Example 2 Comparative Example 2 Example 1 Example 2 Comparative Example 2 Smelly phenomenon improved 4.6 4 3.4 4.6 3.8 3.2 Skin texture improved 4 3.2 2.6 4.4 4 2.8 Dryness symptoms improved 4.2 3.6 2.8 3.8 3.4 2.8 Symptoms such as skin tightness, itchiness, inflammation, and pain are improved. 4 3.8 3 4 3.8 3.2

Claims (4)

6 parts by weight of a mixture of argan oil, jojoba oil, and evening primrose oil; In the cosmetic composition for feminine hygiene management, comprising 4 parts by weight of a mixture of copaiba essential oil and lavender essential oil as active ingredients,
The argan oil, jojoba oil, and evening primrose oil are mixed at 1:1:1 parts by weight, and the copaiba essential oil and lavender essential oil are mixed at 1:1 parts by weight, and a cosmetic composition for feminine hygiene management and the same. Manufacturing method. The method of claim 1, wherein the additional ingredients include tea tree, clary sage, Frankincense, Commiphora Myrrha, Neroli, rose, Geranium, Ylang Ylang, A cosmetic composition for feminine hygiene management, further comprising extracts of jasmine, jojoba, acacia flowers, chamomile, vetiver, or a combination thereof. The method according to claim 1, formulated with one selected from the group consisting of oil, softening lotion, astringent lotion, nourishing lotion, lotion, nourishing cream, massage cream, essence, cleansing cream, cleansing foam, cleansing water, pack, spray, and powder. Cosmetic composition for feminine hygiene management. The cosmetic composition for feminine hygiene management according to claim 1, wherein the cosmetic composition for feminine hygiene management is a composition for improving or preventing vaginitis or groin inflammation.