KR102014102B1 - Composition for Improving Skin Beauty Comprising Mesquite Honey - Google Patents

Abstract

The present invention provides a cosmetic composition for improving skin care, comprising mesquite honey as an active ingredient.

Description

Composition for Improving Skin Beauty Containing Mesquite Honey {Composition for Improving Skin Beauty Comprising Mesquite Honey}

The present invention relates to a cosmetic composition, quasi-drug composition, and pharmaceutical composition for improving skin beauty, which has an effect on skin regeneration and elasticity by using mesquite honey, which is desert honey produced in the desert of Arizona, USA.

Mesquite is the most common legume in northern Mexico and parts of the United States. Widely distributed in the Arizona desert, the roots extend from 45 to 60 meters in the ground, soak up the water from the ground and store it in the drought. Mesquite nectar is made with wheat that blooms from mesquite trees.

On the other hand, honey has long been closely associated with humanity. It has been used as a dietary supplement or as a medicine to replenish your fitness. Although there are differences in ingredients depending on the source of wheat, it is a nutritious food that is effective in recovering fatigue as it contains sugar ingredients such as dextrose, maltose, fructose and sucrose, vitamins and minerals. As medicines, the anti-inflammatory and antimicrobial effects of trauma such as wounds and burns, sore throat and eye inflammation have been scientifically proven.

Korean Laid-Open Patent Publication No. 1999-0070313 relates to a cosmetic composition having excellent moisturizing power using a honey extract, and discloses a cosmetic composition having a high moisturizing effect containing a honey extract.

However, the efficacy of the cosmetics using the conventional honey extract was mainly limited to the moisturizing effect. Thus, the present inventors have made diligent research efforts to improve the action effect of the conventional cosmetic composition for skin protection, when using mesquite honey as an active ingredient for skin improvement action has a skin regeneration and cell proliferation effect, collagen synthesis and elastin The present invention was completed by confirming that the synthesis was promoted.

Korean Laid-Open Patent Publication No. 1999-0070313

It is an object of the present invention to provide a cosmetic composition, quasi-drug composition and pharmaceutical composition for improving skin beauty comprising mesquite honey as an active ingredient.

One embodiment of the present invention provides a cosmetic composition for improving skin care, comprising mesquite honey as an active ingredient.

The cosmetic composition may comprise 0.001 ~ 10.0% by weight of mesquite honey.

In addition, the mesquite honey may contain 36% to 42%, preferably 39% to 41% of revulose.

The cosmetic composition is a toner formulation, and may further include one or more selected from the group consisting of glycerin, butylene glycol, polyoxyethylene hardened castor oil, polysorbate 80, ethanol, preservatives, fragrances, and purified water.

The cosmetic composition is a lotion formulation, glycerin, butylene glycol, glyceryl stearate / page-100 stearate, polysorbate 60, sorbitan sesquioleate, squalane, caprylic / capric triglyceride Light, shea butter, stearyl alcohol, stearic acid, carboxyvinyl polymer, arginine, preservatives, fragrances, purified water may further include one or more selected from the group consisting of.

The cosmetic composition is a cream formulation, glycerin, butylene glycol, glyceryl stearate / page 100 stearate, polysorbate 60, sorbitan stearate, phytosqualane, jojoba oil, caprylic / capric It may further comprise one or more selected from the group consisting of triglycerides, aloe butter, beeswax, stearyl alcohol, behenyl alcohol, stearic acid, carboxyvinyl polymer, arginine, dimethicone, preservatives, fragrances, and purified water.

Another embodiment of the present invention provides a quasi-drug composition for improving skin care, comprising mesquite honey as an active ingredient.

The quasi-drug composition may comprise 0.001 ~ 10.0% by weight of mesquite honey.

Another embodiment of the present invention provides a pharmaceutical composition for improving skin care, comprising mesquite honey as an active ingredient.

The pharmaceutical composition may comprise 0.001 ~ 10.0% by weight of mesquite honey.

The composition for improving skin care using mesquite honey of the present invention as an active ingredient exhibits skin regeneration and cell proliferation effects, and promotes collagen synthesis and elastin synthesis, thereby improving skin elasticity.

Figure 1 is an embodiment of the present invention, a photograph showing the results of the cell regeneration effect on the damaged skin.
Figure 2 is an embodiment of the present invention, a graph showing the regeneration effect of damaged skin cells over time.
3 is a graph showing the results of the cell proliferation effect as an embodiment of the present invention.
Figure 4 shows an example of the effect of increasing the expression of collagen synthase, as an embodiment of the present invention.
Figure 5 shows an example of the effect of increasing the expression of elastin synthase as an embodiment of the present invention.
6 shows an example of the effect of increasing skin moisture as an embodiment of the present invention.
7 is an embodiment of the present invention, the results for the skin wrinkle reduction effect.

One embodiment of the present invention provides a composition for improving skin beauty comprising mesquite honey produced in the Arizona desert as an active ingredient. Mesquite honey has a higher content of Levulose (40.4%) than other honeys, so it can be expected to have a moisturizing effect, skin regeneration and cell proliferation effects, and promote collagen synthesis and elastin synthesis. As a result, skin elasticity improving effect can be exhibited.

In addition, the cosmetic composition for improving skin beauty of the present invention contains 0.001 to 10.0% by weight of mesquite honey as an active ingredient based on the total cosmetic composition. When the content is less than 0.001% by weight, the effect exhibited by mesquite honey is too weak, and when the content exceeds 10.0% by weight, there is a problem in that the stability of the formulation is lowered. We define 100 times the concentration of the active ingredient in the in vitro assay as the effective concentration in the formulation. Therefore, the preferred amount of mesquite honey is 0.1 to 5.0% by weight based on the total cosmetic composition.

The cosmetic composition for improving skin care according to the present invention may further include conventional auxiliaries and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments, flavors, and the like, which are commonly used. For example, the cosmetic composition may further include auxiliary ingredients such as glycerin, butylene glycol, polyoxyethylene hardened castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate, allantoin, and the like. Zero may include hexanediol, purified water, and the like.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art. Specifically, the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, gel, It may be one or more formulations selected from the group consisting of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions, body oils and body cleansers.

For example, when the formulation of the present invention is a paste, cream or gel, the carrier component may include animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, zinc oxide, and the like. have.

As another example, when the formulation of the present invention is a powder or a spray, a carrier component may include lactose, silica, aluminum hydroxide, calcium silicate, polyamide powder, and the like, in particular, in the case of a spray, additionally chlorofluorohydrocarbon, Propellants such as propane / butane, dimethyl ether, and the like.

As another example, when the formulation of the present invention is a solution or an emulsion, a carrier component may include a solvent, a solubilizer, an emulsifier, and the like, and specifically, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, and benzyl benzo. Ate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, and the like.

On the other hand, when the formulation of the present invention is a suspension, a carrier diluent such as water, ethanol, propylene glycol, etc .; Suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar, trachant and the like.

On the other hand, when the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosy Acetates, fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, ethoxylated glycerol fatty acid esters, and the like.

More specifically, when the formulation is a toner, it may further include one or more selected from the group consisting of glycerin, butylene glycol, polyoxyethylene (60) cured castor oil, polysorbate 80, ethanol, preservatives, fragrances, purified water Can be.

In addition, when the formulation is a lotion, glycerin, butylene glycol, glyceryl stearate / fig-100 stearate, polysorbate 60, sorbitan sesquioleate, squalane, caprylic / capric triglycerite, It may further comprise one or more selected from the group consisting of shea butter, stearyl alcohol, stearic acid, carboxyvinyl polymer, arginine, preservatives, flavoring, purified water.

In addition, when the formulation is a cream, glycerin, butylene glycol, glyceryl stearate / fig-100 stearate, polysorbate 60, sorbitan stearate, phytosqualane, jojoba oil, caprylic / capric triglyceride Light, aloe butter, beeswax, stearyl alcohol, behenyl alcohol, stearic acid, carboxyvinyl polymer, arginine, dimethicone, preservatives, fragrances, and purified water may further include one or more selected.

Another aspect of the present invention provides a functional cosmetic for improving skin care comprising the cosmetic composition.

Another aspect provides a quasi-drug composition for improving skin beauty containing mesquite honey as an active ingredient. As used herein, the term "quasi drug" refers to a fiber, a rubber product or the like used for the purpose of treating, alleviating, treating or preventing a disease of a human or animal, has a weak action on the human body or does not directly act on the human body, Or non-machinery and the like, or any of the agents used for sterilization, insecticide and similar purposes for the prevention of infectious diseases, for the purpose of diagnosing, treating, reducing, treating or preventing human or animal diseases. This means that the article is not an instrument, machine or device, and that the article is not an instrument, machine or device for the purpose of pharmacologically affecting the structure and function of a person or animal.

When the composition of the present invention is used as an quasi-drug additive, the composition may be added as it is or used with other quasi-drugs or quasi-drug components, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined depending on the intended use.

In addition, the quasi-drug composition may comprise mesquite honey in 0.001-10% by weight, preferably 0.1 to 5.0% by weight relative to the total weight of the quasi-drug composition. If the content is less than 0.001% by weight, it is difficult to achieve skin regeneration or elasticity improvement effect, and when the content is more than 10% by weight, it is difficult to formulate and the feeling of use may be reduced.

The quasi-drug composition of the present invention is not limited thereto, but preferably, a body cleanser, a disinfectant cleaner, a detergent, a kitchen cleaner, a cleaning cleaner, a toothpaste, a gargle, a wet tissue, a detergent, a soap, a hand wash, a hair cleaner, a hair softener, a humidifier filler It may be one or more formulations selected from the group consisting of a mask, an ointment and a filter filler. Except for the difference according to the dosage form, the same contents as described for the cosmetic composition may be applied to the quasi-drug composition of the present invention.

Another aspect provides a pharmaceutical composition for improving skin beauty containing mesquite honey as an active ingredient.

In addition, the pharmaceutical composition may comprise mesquite honey in 0.001-10% by weight, preferably 0.1 to 5.0% by weight relative to the total weight of the pharmaceutical composition. If the content is less than 0.001% by weight, it is difficult to achieve skin regeneration or elasticity improvement effect, and when the content is more than 10% by weight, it is difficult to formulate and the feeling of use may be reduced.

The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier, excipient or diluent. In addition, the pharmaceutical composition of the present invention may be administered orally or parenterally, and the dosage may be appropriately selected according to the age, sex, weight, condition, degree of disease, form of drug, route of administration, and duration of administration. .

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, the examples and experimental examples according to the present invention may be modified in many different forms, and the scope of the present invention should not be construed as being limited to the examples and experimental examples described below. Examples and experimental examples of the present invention are provided to more fully explain the present invention to those skilled in the art.

Example

Example 1: Mesquite Honey

Arizona desert mesquite honey was purchased from Amazon ( www.amazon.com ) and used in experiments (Trader Joes, USA).

Comparative Example 1: Acacia Honey

Acacia honey of the comparative example was used in the experiment to purchase domestic products (East-West Food, Korea).

Experimental Example 1 Skin Cell Regeneration Effect

In order to confirm the cell regeneration effect in human fibroblasts (Hs68 fibroblast), human fibroblasts were inoculated in a 6 well cell culture dish at a density of 4 × 10 ⁵ , and then cultured in a 37 ° C., 5% CO ₂ incubator for 24 hours. After scraping the cultured cell center using a cell scrapper, the cells were cultured for 24 hours by adding 100 ug / ml of Comparative Example 1 and Example 1 and photographed using a fluorescence microscope.

The results are shown in FIGS. 1 and 2. FIG. 1 is a photograph of cultured wound fibroblasts at 24 hours, and FIG. 2 shows the number of cells proliferated by the wound fibroblasts by time, and the number of cells was measured by a cell counter. From this, when the composition containing the mesquite honey of the present invention (Example 1) was treated, it was not only effective compared to the untreated group, but also treated with the composition containing the acacia honey (Comparative Example 1). It can be seen that it shows a higher skin cell regeneration effect than the group.

Experimental Example 2: Skin Cell Proliferation Effect

The effect of promoting cell proliferation in human fibroblasts was measured by the measurement method using MTT (3- (4,5-dimethylthiazol-2yl) -2,5-diphenyltetrazolium bromide) reagent, and the results are shown in FIG. 3.

Specifically, Hs68 fibroblast, a human fibroblast line, was dispensed in a 96 well plate at a concentration of 2 × 10 ⁴ cells / well, and then cultured in an incubator at 37 ° C. and 5% CO ₂ for 24 hours. Thereafter, the mixture was replaced with a medium to which Comparative Example 1 or Example 1 was added at a concentration of 100 ug / ml, and then further cultured for 24 hours. After incubation, the medium of each well was removed, and the DPBS washing process was performed once, followed by reaction at 37 ° C. for 4 hours by the addition of 0.5 mg / ml MTT reagent. Formazan crystals generated during the reaction were removed by MTT reagent, dissolved in DMSO, and measured at 590 nm using an ELISA reader. Cell proliferation was expressed as a percentage of the mean absorbance values of the untreated group without any sample.

From this, when the composition containing the mesquite honey of the present invention (Example 1) was treated, it was not only effective compared to the untreated group, but also treated with the composition containing the acacia honey (Comparative Example 1). It can be seen that it shows a higher skin cell proliferation effect than the group.

Experimental Example 3: Collagen Expression Increase Effect

Human fibroblast line Hs68 was dispensed into 6 well plates by 4 × 10 ⁵ , followed by incubation for 24 hours in an incubator at 37 ° C. and 5% CO ₂ . After removing the medium and putting DPBS, Example 1 or Comparative Example 1 was added to each 100 ug / ml concentration was further incubated for 24 hours. Then, RNA was isolated from each cell treated with Trizol (RNA iso, DAKARA, Japan), quantified RNA at 260 nm using nanodrop, and then 2 ㎍ of RNA was used in the amplifier. cDNA was synthesized (C1000 Thermal Cycler, Bio-Rad, USA). Real-time polymerase chain reaction was carried out in a real-time PCR machine using a mixture of the synthesized cDNA, the collagen precursor synthesis gene COL1A1 template and the cyanine dye cyberrin (SYBR Green supermis, Applied Biosystems, USA). Finally, the expression level of the COL1A1 gene was evaluated. The sequence and reaction conditions of the template were as shown in Table 1 below, and the expression level of the gene was finally analyzed through correction for the β-actin gene. The result is shown in FIG.

Primer order Reaction conditions COL1A1 F 5'-CACCAATCACCTGCGGTACAGAA-3 ' After activating the polymerase at 94 ° C. for 5 minutes, 40 cycles of polymerization were performed at 95 ° C. 30 seconds, 55 ° C. 30 seconds, and 72 ° C. 30 seconds. R 5'-CAGATCACGTCATCGCACAAC-3 ' β-Actin F 5’-GGCCATCTCTTGCTCGAAGT-3 ’ R 5’-GAGACCTTCAACACCCCAGC-3 ’

Experimental Example 4 Elastin Expression Increase Effect

Human fibroblast line Hs68 was dispensed into 6 well plates by 4 × 10 ⁵ , followed by incubation for 24 hours in an incubator at 37 ° C. and 5% CO ₂ . After removing the medium and putting DPBS, Example 1 or Comparative Example 1 was added to each 100 ug / ml concentration was further incubated for 24 hours. Then, RNA was isolated from each cell treated with Trizol (RNA iso, DAKARA, Japan), quantified RNA at 260 nm using nanodrop, and then 2 ㎍ of RNA was used in the amplifier. cDNA was synthesized (C1000 Thermal Cycler, Bio-Rad, USA). Real-time polymerase chain reaction was carried out in a real-time PCR machine using a mixture of the synthesized cDNA, a target protein elastin synthesis gene ELN template and cyanine dye cyberrin (SYBR Green supermis, Applied Biosystems, USA) Finally, the expression level of the ELN gene was evaluated. The sequence and reaction conditions of the template were as shown in Table 2 below, and the expression level of the gene was finally analyzed through correction for the β-actin gene. The result is shown in FIG.

Primer order Reaction conditions ELN F 5'-GGCCATTCCTGGTGGAGTTCC-3 ' After activating the polymerase at 94 ° C. for 5 minutes, 40 cycles of polymerization were performed at 95 ° C. 30 seconds, 55 ° C. 30 seconds, and 72 ° C. 30 seconds. R 5'-AACTGGCTTAAGAGGTTTGCCTCCA-3 ' β-Actin F 5’-GGCCATCTCTTGCTCGAAGT-3 ’ R 5’-GAGACCTTCAACACCCCAGC-3 ’

As shown in Figures 4 and 5, Example 1 in the collagen synthesis and elastin synthesis effect of the elastic factor, not only effective compared to the untreated group, but also treated with a composition containing a common acacia honey (Comparative Example 1) It showed a better effect.

From these results, when the mesquite honey of the present invention is used as a cosmetic composition, there is an effect of increasing skin regeneration and proliferation, and collagen synthesis and elastin synthesis, so the mesquite honey of the present invention is a composition for improving skin beauty for various uses. It can be seen that it can be used.

Production Example

To the cosmetic composition of the essence formulation as a composition ratio of Table 3 below. In Example 2, the same mesquite honey was used as in Example 1, and Comparative Example 2 was the same as Example 2 except that mesquite honey was not added.

number Raw material Example 2 Comparative Example 2 One Mesquite Honey 10 0 2 EDTA-2Na 0.01 0.01 3 GLYCERINE 99.5% USP 5 5 4 Propanediol 7 7 5 1,2-Hexanediol 2 2 6 Purified water Remaining amount Remaining amount

Experimental Example 5: Measurement experiment of skin moisturizing and wrinkle improvement effect

Example 2 and Comparative Example 2 were divided into half of the face in the morning and evening, and applied for 4 weeks to measure skin moisturizing and wrinkle improvement. Seven adults with an average age of 26.8 years were enrolled.

The degree of moisturizing improvement was measured on Corneometer (CK electronic, Germany) after waiting for 10 minutes in a constant temperature and humidity room after washing the test area (face) with water at 0 days, 2 weeks, and 4 weeks. As the skin moisture level increased, it was judged that the skin moisturization improved.

The degree of wrinkle improvement was measured in Antera 3D (Miravex, UK) after waiting for 10 minutes in a constant temperature and humidity room after washing the test area (face) with water at 0 days, 2 weeks, and 4 weeks. The degree of wrinkle improvement was measured by the indentation of wrinkles by parking. Indentation (A.U) represents the average degree of subsidence of the skin in the designated area, and it was determined that wrinkles were improved as the value decreased.

As shown in FIG. 6, Example 2 to which mesquite honey was added showed high skin moisture (%) after 4 weeks, compared to Comparative Example 2, which was no additive. In addition, as shown in Figure 7, Example 2 was found to improve the skin wrinkles after 4 weeks, compared to Comparative Example 2.

Claims (10)

As a cosmetic composition for improving skin elasticity, comprising mesquite honey as an active ingredient,
The mesquite honey is to contain 0.001 to 10.0% by weight,
The mesquite honey is characterized in that to increase the expression of collagen and elastin gene, composition. delete The method of claim 1,
The mesquite honey contains 36% to 42% of Levulose. The method of claim 1,
The formulation is a toner, and further comprises at least one selected from the group consisting of glycerin, butylene glycol, polyoxyethylene cured castor oil, polysorbate 80, ethanol, preservatives, fragrances, purified water. The method of claim 1,
Formulations are lotions, glycerin, butylene glycol, glyceryl stearate / fig-100 stearate, polysorbate 60, sorbitan sesquioleate, squalane, caprylic / capric triglycerite, shea butter , Stearyl alcohol, stearic acid, carboxylvinyl polymer, arginine, preservatives, fragrances, purified water, at least one selected from the group consisting of, the composition. The method of claim 1,
The formulation is a cream, glycerin, butylene glycol, glyceryl stearate / fig-100 stearate, polysorbate 60, sorbitan stearate, phytoscualan, jojoba oil, caprylic / capric triglyceride, Aloe butter, beeswax, stearyl alcohol, behenyl alcohol, stearic acid, carboxyvinyl polymer, arginine, dimethicone, preservatives, fragrances, and purified water further comprises a cosmetic composition. As an quasi-drug composition for improving skin elasticity, comprising mesquite honey as an active ingredient,
The mesquite honey is to contain 0.001 to 10.0% by weight,
The mesquite honey is characterized in that to increase the expression of collagen and elastin gene, composition. delete delete delete

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