KR20190028082A - Composition for Improving Skin Beauty Comprising Mesquite Honey - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin care, containing Mesquite honey as an active ingredient, which exhibits an effect of improving skin elasticity. The cosmetic composition comprises 0.001 to 10.0 wt% of Mesquite honey.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for improving skin beauty comprising mesquite honey,

The present invention relates to a cosmetic composition, a quasi-drug composition, and a pharmaceutical composition for improving skin beauty, which exhibits an effect on regeneration and elasticity of skin using Mesquite honey, a desert honey produced in the Arizona desert, USA.

Mesquite trees are the most common legumes in northern Mexico and some parts of the United States. It is widely distributed in the Arizona desert, and its roots stretch down to 45 to 60 meters below the ground from the surface of the earth and suck water from underground water. Mesquite honey is made from the flowers blooming in Mesquite trees.

On the other hand, honey has long been closely related to mankind. It has been used as a nutritional food or as a medicinal product to supplement physical fitness. It is a nutritional food which is effective for fatigue recovery by containing sugar components such as dextrose, maltose, fructose, sucrose and vitamins and minerals though there are differences in ingredients depending on wheat origin. As medicines, it has been scientifically proved that anti-inflammation and antibacterial action against trauma and sore throat such as scars and burns, eye inflammation and the like.

Korean Patent Laid-Open Publication No. 1999-0070313 relates to a cosmetic composition having excellent moisture retention using a honey extract, which discloses a cosmetic composition showing a high moisturizing effect containing a honey extract.

However, the efficacy of cosmetics using conventional honey extracts was limited mainly to the moisturizing effect. Accordingly, the present inventors have made extensive efforts to improve the action and effect of the conventional cosmetic composition for skin protection. As a result, the inventors of the present invention have found that when mesquite honey is used as an active ingredient for the skin improving action, it has skin regeneration and cell proliferation effect, The present invention has been completed.

Korean Patent Publication No. 1999-0070313

An object of the present invention is to provide a cosmetic composition for improving skin beauty, a quasi-drug composition and a pharmaceutical composition comprising mesquite honey as an active ingredient.

One embodiment of the present invention provides a cosmetic composition for improving skin beauty, comprising mesquite honey as an active ingredient.

The cosmetic composition may contain 0.001 to 10.0% by weight of Mesquite honey.

The mesquite honey may contain levulose in an amount of 36 to 42%, preferably 39 to 41%.

The cosmetic composition may be at least one selected from the group consisting of glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, polysorbate 80, ethanol, preservative, fragrance, and purified water.

The cosmetic composition is in the form of a lotion agent and is selected from the group consisting of glycerin, butylene glycol, glyceryl stearate / fat-100 stearate, polysorbate 60, sorbitan sesquioleate, squalane, caprylic / capric triglyceride And may further comprise at least one member selected from the group consisting of light, shea butter, stearyl alcohol, stearic acid, carboxyl vinyl polymer, arginine, preservative, fragrance, purified water.

The cosmetic composition of the present invention is in the form of a cream, and is in the form of glycerin, butylene glycol, glyceryl stearate / fat-100 stearate, polysorbate 60, sorbitan stearate, phytosqualene, jojoba oil, caprylic / It may further comprise at least one selected from the group consisting of triglyceride, aloe butter, bees wax, stearyl alcohol, behenyl alcohol, stearic acid, carboxyl vinyl polymer, arginine, dimethicone, preservative, fragrance and purified water.

Another embodiment of the present invention provides a quasi-drug composition for improving skin care, which comprises mesquite honey as an active ingredient.

The quasi-drug composition may contain 0.001 to 10.0% by weight of mesquite honey.

Another embodiment of the present invention provides a pharmaceutical composition for skin aesthetic improvement comprising mesquite honey as an active ingredient.

The pharmaceutical composition may comprise from 0.001 to 10.0% by weight of Mesquite honey.

The skin cosmetic composition containing the scalpel honey as an active ingredient of the present invention exhibits skin regeneration and cell proliferation effects and promotes collagen synthesis and elastin synthesis, thereby exhibiting skin elasticity improving effect.

Fig. 1 is a photograph showing the result of cell regeneration effect in damaged skin as an embodiment of the present invention. Fig.
2 is a graph showing the regeneration effect of damaged skin cells over time as an embodiment of the present invention.
FIG. 3 is a graph showing the results of cell proliferation effect as an embodiment of the present invention. FIG.
Fig. 4 shows the results of the effect of increasing expression of collagen synthase as an embodiment of the present invention.
FIG. 5 shows the results of increasing expression of elastin synthase as an embodiment of the present invention.
FIG. 6 shows the result of skin moisture increasing effect as an embodiment of the present invention.
FIG. 7 shows the results of skin wrinkle reducing effect as one embodiment of the present invention.

One embodiment of the present invention provides a composition for improving skin care comprising mesquite honey produced in an Arizona desert as an active ingredient. Mesquite honey has a high content of levulose (40.4%) compared to other honey, and it can not only provide a moisturizing effect but also has skin regeneration and cell proliferation effect and promotes collagen synthesis and elastin synthesis The skin elasticity can be improved.

In addition, the cosmetic composition for skin beauty improvement of the present invention contains 0.001 to 10.0% by weight of Mesquite honey as an active ingredient based on the whole cosmetic composition. When the content is less than 0.001% by weight, the effect caused by the scalpel honey is too weak, and when the content exceeds 10.0% by weight, the stability of the formulation is lowered. The present inventors define the effective concentration in the formulation as 100 times the concentration of the active ingredient in the in vitro assay. Thus, the preferred amount of Mesquite honey is 0.1-5.0 wt.%, Based on the total cosmetic composition.

The cosmetic composition for skin care improvement of the present invention may further contain conventional auxiliary agents and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments, perfumes and the like which are conventionally used. For example, the cosmetic composition may further contain auxiliary components such as glycerin, butylene glycol, polyoxyethylene hardened castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate, allantoin, Zero may include hexanediol, purified water, and the like.

The cosmetic composition of the present invention can be prepared into any formulation conventionally produced in the art. Specifically, the cosmetic composition may be a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, A pack, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion, a body oil and a body cleanser.

For example, when the formulation of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, have.

For example, when the formulation of the present invention is a powder or a spray, the carrier component may include lactose, silica, aluminum hydroxide, calcium silicate, polyamide powder and the like. Particularly, in the case of a spray, Propane / butane, dimethyl ether, and the like.

As another example, when the formulation of the present invention is a solution or an emulsion, the carrier component may include a solvent, a solubilizing agent, an emulsifying agent, and the like. Specific examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, Glycolate, glycerol aliphatic ester, polyethylene glycol, fatty acid esters of sorbitan, and the like.

On the other hand, when the formulation of the present invention is a suspension, a liquid diluent such as water, ethanol, propylene glycol or the like as the carrier component; Suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, trachant, and the like.

On the other hand, when the formulation of the present invention is an interface-active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyltaurate, Fatty acid amide ether sulfate, alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, ethoxylated glycerol fatty acid ester, and the like.

More specifically, when the formulation is a toner, it further comprises at least one selected from the group consisting of glycerin, butylene glycol, polyoxyethylene (60) hardened castor oil, polysorbate 80, ethanol, preservative, perfume, purified water .

Also, when the formulation is a lotion agent, it is possible to use glycerin, butylene glycol, glyceryl stearate / fat-100 stearate, polysorbate 60, sorbitan sesquioleate, squalane, caprylic / capric triglyceride, But may further include at least one selected from the group consisting of shea butter, stearyl alcohol, stearic acid, carboxyl vinyl polymer, arginine, preservative, fragrance, purified water.

Also, when the formulation is a cream, it may also contain glycerin, butylene glycol, glyceryl stearate / fat-100 stearate, polysorbate 60, sorbitan stearate, phytosqualan, jojoba oil, caprylic / capric triglyceride And may further comprise at least one member selected from the group consisting of water, aloe vera, beeswax, aloe vera, beeswax, stearyl alcohol, behenyl alcohol, stearic acid, carboxyvinyl polymer, arginine, dimethicone, preservative, fragrance and purified water.

Another aspect of the present invention provides a functional cosmetic for improving skin beauty comprising the cosmetic composition described above.

Another aspect provides a quasi-drug composition for skin aesthetic improvement comprising mesquite honey as an active ingredient. The term "quasi-drug" in the present invention means a fiber, a rubber product or the like used for the purpose of treating, alleviating, treating or preventing a disease of a human or an animal, Or products similar to those that are not machinery, and those used for sterilization, insecticide and similar uses for the prevention of infectious diseases, for the purpose of diagnosis, treatment, alleviation, treatment or prevention of diseases of human or animal. Machinery, or apparatus, and that is not an apparatus, machine, or apparatus of an article used for the purpose of causing a pharmacological effect on the structure or function of a person or animal.

When the composition of the present invention is used as a quasi-drug additive, the composition may be added as it is or may be used together with other quasi-drugs or quasi-drugs, and may be appropriately used according to a conventional method. The amount of the active ingredient to be mixed can be appropriately determined depending on the purpose of use.

In addition, the quasi-drug composition may contain Mesquite honey in an amount of 0.001-10% by weight, preferably 0.1-5.0% by weight, based on the total weight of the composition. When the content is less than 0.001% by weight, it is difficult to achieve skin regeneration or elasticity improvement effect. When the content is more than 10% by weight, formulation may be difficult and feeling of use may be deteriorated.

The quasi-drug composition of the present invention is not limited thereto, but preferably includes a body cleanser, a disinfectant cleaner, a detergent, a kitchen detergent, a cleaning detergent, a toothpaste, a gauze agent, a wet tissue, a detergent, a soap, a hand wash, , A mask, an ointment agent, and a filter filler. In the quasi-drug composition of the present invention, the same contents as described for the cosmetic composition may be applied, except for differences according to the formulation.

Another aspect provides a pharmaceutical composition for improving skin beauty, comprising mesquite honey as an active ingredient.

In addition, the pharmaceutical composition may contain Mesquite honey in an amount of 0.001-10% by weight, preferably 0.1-5.0% by weight, based on the total weight of the pharmaceutical composition. When the content is less than 0.001% by weight, it is difficult to achieve skin regeneration or elasticity improvement effect. When the content is more than 10% by weight, formulation may be difficult and feeling of use may be deteriorated.

The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier, excipient or diluent. In addition, the pharmaceutical composition of the present invention can be administered orally or parenterally, and the dose can be appropriately selected depending on the age, sex, weight, condition, degree of disease, form of the drug, .

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, the embodiments and experimental examples according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the above-described embodiments and examples. The embodiments and experimental examples of the present invention are provided to enable those skilled in the art to more fully understand the present invention.

Example

Example 1: Mesquite honey

Arizona desert mesquite honey was purchased from Amazon ( www.amazon.com ) and used for the experiment (Trader Joes, USA).

Comparative Example 1: Acacia honey

Acacia honey of Comparative Example was purchased from domestic sources (Dongseo Foods, Korea) and used in the experiment.

Experimental Example 1: Skin cell regeneration effect

Human fibroblasts were inoculated at a density of 4 × 10 ⁵ cells in a 6-well cell culture dish and cultured in a 5% CO ₂ incubator at 37 ° C. for 24 hours in order to confirm cell regeneration effect in human fibroblast (Hs68 fibroblast). Cell scrapper was used to scrape the center of the cultured cells. 100 .mu.g / ml of each of Comparative Example 1 and Example 1 was added thereto for 24 hours, and the degree of cell proliferation was photographed using a fluorescence microscope.

The results are shown in Fig. 1 and Fig. FIG. 1 is a photograph of wounded fibroblasts at 24 hours of culture, and FIG. 2 shows the number of cells proliferated over time in wounded fibroblasts. Cell counts were measured with a cell counter instrument. From this, it can be seen that when the composition containing the scalpel honeycomb of the present invention (Example 1) is treated, it is more effective than the untreated group and the composition containing ordinary acacia honey (Comparative Example 1) is treated It can be confirmed that skin cell regeneration effect is higher than that of the control group.

Experimental Example 2: Effect of skin cell proliferation

The presence or absence of the cell proliferation promoting effect in human fibroblasts was measured by a measurement method using MTT (3- (4,5-dimethylthiazol-2yl) -2,5-diphenyltetrazolium bromide) reagent.

Specifically, human fibroblast Hs68 fibroblast was dispensed into 96-well plates at a concentration of 2 × 10 ⁴ cells / well and cultured in an incubator at 37 ° C and 5% CO ₂ for 24 hours. Then, the medium was replaced with medium supplemented with Comparative Example 1 or Example 1 at a concentration of 100 ug / ml, followed by further incubation for 24 hours. After the incubation, the medium of each well was removed and the DPBS washing procedure was performed once. Then, 0.5 mg / ml of MTT reagent was added and reacted at 37 ° C for 4 hours. Formazan crystals formed during the reaction were dissolved in DMSO after removing the MTT reagent, and then measured at 590 nm using an ELISA reader. Cell proliferation was expressed as a percentage of the average absorbance value of the untreated group without any sample.

From this, it can be seen that when the composition containing the scalpel honeycomb of the present invention (Example 1) is treated, it is more effective than the untreated group and the composition containing ordinary acacia honey (Comparative Example 1) is treated The skin cell proliferation effect is higher than that of the control group.

Experimental Example 3: Increase in collagen expression

The human fibroblast cell line Hs68 was divided into 4 × 10 ⁵ cells in a 6-well plate and cultured in an incubator at 37 ° C. and 5% CO ₂ for 24 hours. After the medium was removed, DPBS was added, and then 100 μg / ml of Example 1 or Comparative Example 1 was added thereto, followed by further culturing for 24 hours. Then, RNA was isolated from the cells treated with each sample using triazole (RNA iso, DAKARA, Japan), and RNA was quantitated at 260 nm using nanodrop. Then, 2 μg of each RNA was used for amplification cDNA was synthesized (C1000 Thermal Cycler, Bio-Rad, USA). Real-time PCR was performed in a real-time PCR machine using a mixture of the COL1A1 template, a synthetic collagen precursor, and cyber green (SYBR Green supermis, Applied Biosystems, USA) And the degree of expression of the COL1A1 gene was finally evaluated. The sequence of the template and the reaction conditions were as shown in Table 1 below, and the expression amount of the gene was finally analyzed by correcting for the β-actin gene. The results are shown in Fig.

Primer order Reaction conditions COL1A1 F 5'-CACCAATCACCTGCGGTACAGAA-3 ' After activation of the polymerase at 94 ° C for 5 minutes, polymerization was carried out at 40 ° C for 30 seconds at 95 ° C for 30 seconds, at 55 ° C for 30 seconds, and at 72 ° C for 30 seconds R 5'-CAGATCACGTCATCGCACAAC-3 ' β-Actin F 5'-GGCCATCTCTTGCTCGAAGT-3 ' R 5'-GAGACCTTCAACACCCCAGC-3 '

Experimental Example 4: Increasing effect of elastin expression

The human fibroblast cell line Hs68 was divided into 4 × 10 ⁵ cells in a 6-well plate and cultured in an incubator at 37 ° C. and 5% CO ₂ for 24 hours. After the medium was removed, DPBS was added, and then 100 μg / ml of Example 1 or Comparative Example 1 was added thereto, followed by further culturing for 24 hours. Then, RNA was isolated from the cells treated with each sample using triazole (RNA iso, DAKARA, Japan), and RNA was quantitated at 260 nm using nanodrop. Then, 2 μg of each RNA was used for amplification cDNA was synthesized (C1000 Thermal Cycler, Bio-Rad, USA). Real-time PCR was performed in a real-time PCR machine using a mixture of an ELN template, an elastin synthetic gene, and a cyanine dye (SYBR Green supermis, Applied Biosystems, USA) Finally, the expression level of ELN gene was evaluated. Sequence and reaction conditions of the template were as shown in Table 2 below, and the amount of gene expression was finally analyzed by correcting for β-actin gene. The results are shown in Fig.

Primer order Reaction conditions ELN F 5'-GGCCATTCCTGGTGGAGTTCC-3 ' After activation of the polymerase at 94 ° C for 5 minutes, polymerization was carried out at 40 ° C for 30 seconds at 95 ° C for 30 seconds, at 55 ° C for 30 seconds, and at 72 ° C for 30 seconds R 5'-AACTGGCTTAAGAGGTTTGCCTCCA-3 ' β-Actin F 5'-GGCCATCTCTTGCTCGAAGT-3 ' R 5'-GAGACCTTCAACACCCCAGC-3 '

As shown in Figs. 4 and 5, Example 1 is effective for collagen synthesis and elastin synthesis effect, which are elasticity factors, as compared with the untreated group, and also to a group treated with a composition containing ordinary acacia honey (Comparative Example 1) And showed a better effect.

From these results, it has been found that when the mesquite honey of the present invention is used as a cosmetic composition, skin regeneration and proliferation, and collagen synthesis and elastin synthesis increase, the mesquite honey of the present invention is a composition for improving skin beauty for various purposes Can be used.

Manufacturing example

Cosmetic composition of essence formulation was prepared as composition ratio shown in Table 3 below. In Example 2, the same Mesquite honey as in Example 1 was used, and in Comparative Example 2, it was the same as Example 2 except that Mesquite honey was not added.

number Raw material Example 2 Comparative Example 2 One Mesquite Honey 10 0 2 EDTA-2Na 0.01 0.01 3 GLYCERINE 99.5% USP 5 5 4 Propanediol 7 7 5 1,2-Hexanediol 2 2 6 Purified water Balance Balance

Experimental Example 5: Experiment to measure skin moisturizing effect and wrinkle improvement effect

Example 2 and Comparative Example 2 were applied to half of the face in the morning and evening for 4 weeks to measure skin moisturizing and wrinkle improvement. Seven adults with an average age of 26.8 years were enrolled.

The degree of moisturizing improvement was measured by the Corneometer (CK electronic, Germany) after washing the test site (face) at 0 day, 2 weeks, and 4 weeks, after waiting for 10 minutes in the constant temperature and humidity room. The skin moisturization was improved as the skin moisture level increased.

The degree of wrinkle improvement was measured by Antera 3D (Miravex, UK) after wiping the test site (face) at 0 day, 2 weeks, and 4 weeks and then standing in a constant temperature and humidity room for 10 minutes. The degree of wrinkle improvement was measured by the indentation value of wrinkles per parking lot. Indentation (A.U) indicates the average degree of settlement of the skin at the designated site, and it was judged that the wrinkles were improved as the value decreased.

As shown in FIG. 6, Example 2, in which Mesquite honey was added, exhibited high skin moisture content (%) after 4 weeks compared to Comparative Example 2 without addition. Also, as shown in Fig. 7, the wrinkles of the skin of Example 2 were improved after 4 weeks as compared with those of Comparative Example 2.

Claims (10)

A cosmetic composition for improving skin beauty, comprising mesquite honey as an active ingredient. The method according to claim 1,
0.001 to 10.0% by weight of Mesquite honey. The method according to claim 1,
Wherein the Mesquite honey comprises 36-42% Levulose. The method according to claim 1,
Wherein the formulation is a toner preparation and further comprises at least one selected from the group consisting of glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, polysorbate 80, ethanol, preservative, perfume, purified water. The method according to claim 1,
The formulations are lotion preparations and may be formulated with one or more of the following: glycerin, butylene glycol, glyceryl stearate / fat-100 stearate, polysorbate 60, sorbitan sesquioleate, squalane, caprylic / capric triglyceride, , Stearyl alcohol, stearic acid, carboxyl vinyl polymer, arginine, preservative, perfume, purified water. The method according to claim 1,
The formulations are formulated in a creamy form and contain at least one of glycerin, butylene glycol, glyceryl stearate / fat-100 stearate, polysorbate 60, sorbitan stearate, phytosqualan, jojoba oil, caprylic / capric triglyceride, Wherein the cosmetic composition further comprises at least one selected from the group consisting of aloe butter, bees wax, stearyl alcohol, behenyl alcohol, stearic acid, carboxyl vinyl polymer, arginine, dimethicone, preservative, fragrance and purified water. A quasi-drug composition for improving skin care, comprising Mesquite honey as an active ingredient. 8. The method of claim 7,
0.001 to 10.0% by weight of Mesquite honey. A pharmaceutical composition for improving skin beauty, comprising mesquite honey as an active ingredient. 10. The method of claim 9,
0.001 to 10.0% by weight of Mesquite honey.

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