KR20140141080A - Composition containing extract using process of herbal medicine - Google Patents

Abstract

The present invention relate to a composition including a ginseng extract of herbal medicine processing and a green tea extract of herbal medicine processing. The composition for skin trouble release and treatment includes one or more mixture of herbal medicine processed ginseng extract and herbal medicine processed green tea selected from a group consisting of a mixture of naturally fermented ginseng polysaccharide and naturally fermented green tea polysaccharide; a mixture of bacteria fermented ginseng polysaccharide and bacteria fermented green tea polysaccharide; and a mixture of salt fermented ginseng polysaccharide and salt fermented green tea polysaccharide as an effective component.

Description

COMPOSITION CONTAINING EXTRACT USING PROCESS OF HERBAL MEDICINE [0002]

The present invention relates to a composition comprising an extract of forage-treated ginseng and an extract of forage-treated green tea.

The external appearance of the skin is the extracellular matrix (ECM) component of the dermis, which is about 70-80% of the total ECM. Creation of the skin wrinkles is caused by degradation or destruction of collagen caused by aging or ultraviolet rays. In particular, the expression of matrix metalloproteases such as collagenase causes collagen to be normally broken down in the skin, resulting in wrinkles.

Human skin is the primary protective membrane of the human body. It functions to protect the internal organs from external environmental stimuli such as changes in temperature and humidity, ultraviolet rays, and pollutants. Depending on various internal and external factors, It undergoes change. In other words, internally, secretion of various hormones that regulate metabolism is reduced, the function of immune cells and the activity of cells are lowered, and the biosynthesis of immune proteins and biocompatible proteins necessary for the living body is reduced, and ozone layer destruction As the content of ultraviolet rays reaching the surface of the sunlight increases and environmental pollution is further intensified, free radicals and active noxious oxygen are increased, so that the thickness of the skin decreases, the wrinkles increase, the elasticity decreases Skin color is grayish, skin troubles occur frequently, and spots, freckles and black blots also increase.

In order to prevent the change of the skin condition due to the intrinsic and extrinsic factors, and to maintain a healthy skin condition, physiologically active substances obtained from various known animals, plants, microorganisms and the like are added to cosmetics to improve the skin condition There has been effort for.

The inventors of the present invention have confirmed that a mixture of Forsythia extract of green ginseng and Forsythiae extract of green tea can improve skin troubles, and particularly has a synergistic effect superior to that of a mixture of common ginseng and green tea extract.

Dermatology therapy, 16, 357-364, 1998

It is an object of the present invention to provide a composition comprising a Fogue extract of a natural product.

In order to accomplish the above object, the present invention provides a composition for reducing pores, controlling sebum, or alleviating or alleviating skin troubles, which comprises a mixture of fog-treated ginseng extract and forage-treated green tea extract as an active ingredient.

The composition of the present invention has remarkably superior skin troubles to alleviate or improve the skin troubles than the mixture of the extracts not treated with the fauge or each of the extracts treated with the fauge, including a mixture of the fauge-treated ginseng extract and the green tea extract. Specifically, the composition of the present invention has a synergistic effect due to the mixing of each of the forage-treated extracts. Thus, the composition of the present invention has anti-inflammatory and anti-allergic effects superior to a mixture of common ginseng extract and green tea extract and has the effect of improving acne and skin troubles and atopic symptoms . It can also provide the effect of pore reduction or sebum control. The composition of the present invention can also inhibit the expression of prostaglandins, inhibit the production of interleukins and thereby alleviate skin itching. In addition, the composition of the present invention has antibacterial activity and can improve atopic symptoms. In addition, the composition of the present invention can reduce the pores and control the amount of sebum produced in the sebaceous glands and released into the skin through the pores, and alleviate or improve the disease or trouble due to sebum secretion. The composition of the present invention contains little or no toxicity and irritation, including natural extracts, and has little side effects in long-term use.

Therefore, the composition of the present invention can provide an overall skin condition improving effect through anti-inflammatory and anti-allergic effect, pore reduction, sebum control, acne and atopy.

The present invention relates to a composition for reducing pores, controlling sebum, or alleviating or alleviating skin troubles, which comprises, as an active ingredient, a mixture of a forage-treated ginseng extract and a green tea extract.

In one aspect of the present invention, the mixture of Foaming Ginseng Extract and Foaming Green Tea Extract is not only a mixed extract obtained by mixing ginseng and green tea, but also a ginseng extract and a green tea extract obtained by separately extracting ginseng and green tea And mixtures thereof.

In the present specification, the term " formulated " means a constraint technique that changes the original properties of a medicament by processing the medicament based on the oriental theory. Foje can include boiling, steaming, roasting, roasting, burning, or boiling for the purpose of reducing the toxicity of the medicinal product, preserving the medicinal properties of the medicinal product, improving the medicinal properties, and improving the convenience of taking the medicinal product. In addition, the foaming method includes, for example, frying the medicinal material, frying the medicinal material with a certain amount of the liquid auxiliary material, grinding the auxiliary material into the medicinal tissue, medicinal material, (Steam) method in which auxiliary materials are added and mixed and heated in a suitable container and steamed or steamed until a certain degree is reached. The spinning method is further subdivided according to the strength of the fire and the type of pile to be used. Some examples are as follows. It is a method of frying medicinal materials until it reaches a level defined by culture (fire) or artificial fire (fire). The liquorice method is a method of roasting a medicinal substance using about 15% of the alcohol and the method of boiling the medicinal substance using about 15% of alcohol. Even in the same medicinal plant, the properties of the raw material and the sown material may not be the same or may differ from each other. In addition, the combined prescription of a combination of raw materials and mature materials can maximize the therapeutic effect by elevating the drug efficacy of each medicinal substance or by canceling the toxicity.

In the present specification, the term " fogging treatment "means pre-treatment of green tea or ginseng with the above-defined " foggy ", and specifically, the fogging method is used in the fogging method. However, You can do this without using. Specifically, in the present specification, the "fogging treatment" is a treatment step comprising a) applying a green tea or ginseng to a high temperature and high pressure; And b) drying the processed green tea or ginseng. However, the present invention is not limited thereto and can be used as long as it is a commonly used casting method in the art.

In the present specification, "ginseng" (Panax ginseng C. A. Meyer) is a plant belonging to the genus Omega and Ginseng. In this specification, the ginseng can be used without limitation in its kind and year, and both dried and non-dried ginseng can be used. Further, in the present specification, the ginseng can be used without limitation in the site, and roots can be used specifically.

In the present specification, "green tea" can be used regardless of its kind and number of growing days, and both dried and not green tea can be used. Also, in the present specification, the ginseng can be used without limitation in the site, and specifically, the leaves of green tea can be used.

In the present specification, "skin trouble" means a case where the skin is not in a normal state due to various causes. Specifically, it includes, but is not limited to, acne, atopy, rash or allergy, and includes swollen or crusted skin.

In the present specification, the term "pore" means a space where hair is held in the skin of the whole body and is also connected to the sebaceous gland. The sebaceous glands attached to the pores secrete sebum. The pores spread to release excess sebum during puberty when the sebaceous glands are excessively secreted. When the sebaceous follicles stay inside the pores in the process of discharging the pores out, the stagnant sebum further widens the pores. Another cause of wider pores is skin aging. Skin aging affects the degeneration or reduction of collagen fibers and elastic fibers, which play a role in supporting the pores. When the force to support the pores is insufficient Pores naturally expand.

As used herein, "sebum" refers to lipids secreted from the sebaceous glands attached to the pores. Although sebum appears to have a physiological function involved in preventing skin dryness and lubrication, excessive secretion of sebum may lead to skin troubles such as acne. In addition, free fatty acids produced by the breakdown of triglycerides by bacteria present in sebaceous parenchyma play an important role in the development of acne.

In the present specification, "natural fermentation" means not allowing fermentation by inoculation of a specific bacterium but fermentation by bacteria existing in a natural state. For example, when ginseng is mixed with oligosaccharide, But not limited thereto.

In the present specification, the term " bacterial fermentation "means fermentation of the bacteria to be inoculated at a constant concentration for a certain period of time while maintaining conditions of temperature, aeration amount, and pH suitable for fermentation. Examples thereof include Saccharomyces sp. At a concentration of about 10 < ⁵ > to 10 < ⁷ > cells / ml and fermenting for 24 to 120 hours while maintaining the conditions of pH 5.5 to 6.5, 25 to 50 DEG C, But is not limited thereto.

In the present specification, "salted fermentation" means fermentation in a salt-pickled state, and may be fermentation by natural fermentation in the fermentation process or by inoculation with bacteria. Such as fermentation for 4 to 5 days by adding 8 to 12% by weight of yeast and 1.5 to 2.5% of salt so as to cover the medicinal material, based on the weight of the medicinal material.

The composition for alleviating or improving skin troubles according to one aspect of the present invention is significantly superior to a mixture of green tea extract and ginseng extract, including a green tea extract and a ginseng extract, Or improved.

In a composition for alleviating or improving skin trouble, which is one aspect of the present invention, the composition may have anti-inflammatory activity. The composition, which is an aspect of the present invention, can alleviate skin irritation and alleviate inflammation, and thus can be used for anti-inflammation. Specifically, the composition of the present invention may have an anti-allergic effect by inhibiting or inhibiting the expression of prostaglandin or interleukin, or by inhibiting or inhibiting the activity of prostaglandin or interleukin. Therefore, in one aspect of the present invention, the composition may be for anti-inflammatory.

 In a composition for alleviating or improving skin troubles which is one aspect of the present invention, the composition may have anti-allergic activity. In one aspect of the present invention, the composition can treat or alleviate allergy by alleviating allergies or by inhibiting mechanisms in the body that cause allergies. Specifically, the composition of the present invention may have an anti-allergic effect by inhibiting or inhibiting the expression of prostaglandin or interleukin, or by inhibiting or inhibiting the activity of prostaglandin or interleukin. Thus, in a composition that is an aspect of the invention, the composition may be for anti-allergy.

In a composition for alleviating or improving skin troubles which is one aspect of the present invention, the composition may treat or ameliorate atopy. The composition, which is an aspect of the present invention, not only has anti-inflammatory and anti-allergic effects, but also can enhance skin barrier function and induce differentiation of dermal keratinocytes, Can be used for the treatment of visible atopy or for alleviation of atopic symptoms. Therefore, in one aspect of the invention, the composition may be for the treatment or improvement of atopy.

In a composition for reducing pores, controlling sebum or alleviating or improving skin troubles, which is one aspect of the present invention, the composition may have antibacterial activity. The composition, which is one aspect of the present invention, specifically shows excellent antibacterial activity against acne bacteria. Therefore, the composition can be effectively used in the treatment and alleviation of acne.

In one aspect of the invention, the composition may be for antimicrobial use.

In a composition for reducing pores, controlling sebum or alleviating or improving skin troubles, which is one aspect of the present invention, the fungus may be acne-like.

The composition of the present invention means to alleviate the skin troubles or skin diseases caused by various reasons, to improve the condition, or to remove or inhibit various reasons causing skin troubles or skin diseases, It can improve the internal condition and is useful for improving skin condition.

In a composition for alleviating or improving skin troubles which is one aspect of the present invention, the composition may inhibit or inhibit the production of prostaglandins. In one aspect of the present invention, the composition can inhibit or inhibit the production of prostaglandin itself, or inhibit or inhibit the activity of prostaglandins. The composition, which is an aspect of the present invention, can also inhibit or inhibit the activity of an up-stream enzyme or protein that produces prostaglandins.

In the composition for alleviating or improving skin troubles which is one aspect of the present invention, the composition may inhibit or inhibit the production of interleukin. In one aspect of the invention, the composition may inhibit or inhibit the production of the interleukin itself or may inhibit or inhibit the activity of the interleukin. The composition, which is an aspect of the present invention, may also inhibit or inhibit the activity of an up-stream enzyme or protein that produces interleukin. Specifically, the interleukin may include interleukin-6 or interleukin-8, but is not limited thereto. The composition of the present invention can also be used as a composition for reducing pores, controlling sebum, and improving skin troubles. It reduces sebum secreted by the skin when applied to the skin, reduces active oxygen and promotes collagen synthesis, The reduction of the expression of inflammatory factors leads to an excellent effect of suppressing skin troubles.

In a composition for controlling pore reduction or sebum, which is an aspect of the present invention, the composition is superior to a mixture of a common green tea extract and a ginseng extract, including a green tea extract and a ginseng extract, Or to regulate the sebum secreted into the skin through the pores.

In the composition for reducing pores or controlling sebum, which is one aspect of the present invention, the composition can provide the effect of shrinking the enlarged pores due to various reasons. In the composition for controlling pore reduction or sebum, which is an aspect of the present invention, the composition can provide an effect of controlling the amount of sebum secreted and released from the sebaceous glands.

More specifically, the composition can reduce the pore opening, reduce the entire pore, inhibit the secretion of sebum in the sebaceous glands, and suppress the amount of sebum secreted into the pores. Also, when the amount of sebum produced is suppressed, the amount of stagnant sebum residing in the pores is also reduced, thereby reducing pores. Due to the above-described effects, the composition for reducing pores or controlling sebum of the present invention can alleviate or improve skin troubles such as acne due to sebum secretion.

In a composition for reducing pores, controlling sebum or alleviating or improving skin troubles, which is one aspect of the present invention, the forage-treated ginseng extract may comprise a ginseng polysaccharide.

In a composition for reducing pores, controlling sebum or alleviating or improving skin troubles, which is one aspect of the present invention, the ginseng polysaccharide may be at least one selected from the group consisting of ginseng natural fermentation polysaccharide, ginseng fermentation polysaccharide, have.

As used herein, the term "ginseng polysaccharide" refers to an effective ingredient derived from ginseng (Panax ginseng CA Meyer), which is a pectin-like substance having a molecular weight of 34,600, accounting for about 60% of galacturonic acid, , Rhamnose, glucose, and galactose constitute side chains.

In a composition for reducing pores, controlling sebum or alleviating or improving skin troubles, which is one aspect of the present invention, the forage-treated green tea extract may comprise a green tea polysaccharide.

In the present specification, "Green tea polysaccharide" is derived from green tea, and is an acidic polysaccharide group, unlike a polysaccharide found in other plants, and is produced by combining sugar components and amino acids produced through photosynthesis.

In a composition for reducing pores, controlling sebum or alleviating or alleviating skin troubles, which is one aspect of the present invention, the green tea polysaccharide may be at least one selected from the group consisting of green tea natural fermentation polysaccharide, green tea bacterial fermentation polysaccharide, and green tea salting fermentation polysaccharide have.

The polysaccharide of the present invention can be produced by a method known in the art, and the method is not particularly limited. Specifically, after extracting ginseng or green tea leaves at 38 to 42 ° C, it is concentrated, and the impurities are removed by a filter. Ethanol is added thereto by dropwise method and hot air drying is performed to obtain ginseng or green tea polysaccharide.

In the present specification, the ginseng or green tea natural fermentation polysaccharide is characterized in that the natural fermentation polysaccharide is obtained by mixing ginseng, green tea or a mixture thereof with an oligosaccharide; Spontaneously fermenting the mixture for 3 to 9 months in Onggi; Removing the impurities and extracting the impurities; And concentrating and drying the extract. For example, ginseng or green tea leaves are mixed with 20 g to 100 g of saccharides, placed in a container, covered with the container in a natural state without applying pressure from above, naturally fermented for 3 to 9 months, removed from the fermented raw material, Then immersing it in water, concentrating it, adding ethanol drop wise thereto, and drying it. Sugars may include, but are not limited to, glucose, fructose, lactose, oligosaccharides, glycogen or starch, and include sugars that can be used as an energy source in fermentation. The container may include, but is not limited to, an iron container, an aluminum container, a plastic container, a stainless container or a pot, which can be selected in proceeding natural fermentation. It is preferable to use pottery for containers.

In the present specification, the ginseng or green tea fermentation polysaccharide is added to ginseng, green tea or a mixture thereof; Inoculating at least one bacterium selected from the group consisting of yeast, mushroom, lactic acid, mycelia, and Bacillus subtilis; Fermenting for 10 to 100 days; Removing the impurities and extracting the impurities; And concentrating and drying the extract. For example, one or more of yeast, green mushroom, lactic acid bacterium, Bacillus subtilis or Bacillus subtilis is inoculated into ginseng or green tea leaves at a concentration of 10 ⁵ to 10 ⁷ cells / ml, and at a temperature of 5 to 8, 10 to 60 ° C, 2 VVM for 10 to 100 days, removing the impurities from the fermented raw material, extracting the fermented raw material, extracting the fermented raw material, concentrating the fermented raw material, adding ethanol to the fermented raw material in a drop wise manner, and drying.

In the present specification, the ginseng or green tea salty fermentation polysaccharide is obtained by aging ginseng, green tea or a mixture thereof with salt; Heating and drying; Treating the brine; Fermenting for 3 to 7 days; Removing the impurities and extracting the impurities; And concentrating and drying the extract. For example, ginseng or green tea leaves are aged for 5 to 10 days with salt, followed by heating and drying, adding salt water, fermenting for 3 to 10 days, removing impurities from the fermented raw material, Followed by addition of ethanol in a dropwise manner, followed by drying. Also, in the present specification, the ginseng or green tea salted fermentation polysaccharide may include a step of inoculating and fermenting a bacterium in the course of fermentation with addition of salt water.

The kind of bacteria that can be used in the present specification is not particularly limited, but one or more kinds of yeast, mushroom, lactic acid bacteria, hwanggukyun, and Bacillus subtilis can be used.

Specific examples of yeasts include Saccharomyces sp. , Schizosaccharomyces sp. , Torulopsis sp. , Rhodotorula sp. , Or Candida sp. And the like can be used.

In addition, mushroom fungi can be any mushroom fungus which can be edible. Preferably, mushroom fungi such as Inonnotus-obliquus , Cordyceps sinensis, Paecilomyces japonica , Tricholomamatsutake , Phellinus linteus , Or ganoderma lucidum may be used.

As the lactic acid bacteria, bacteria such as Lactobacillus sp. , Streptococcus sp. , Leuconostoc sp. , Or Bifidobacteria sp. Can be used.

In addition, the nurukgyun may be a bacterium, such as rayijo crispus (Rhyzopus), Usami (Usami) and duck material (Oryzae), Aspergillus duck material (Aspergillus oryzae), Aspergillus material (Aspergillus sojae).

Moreover, Bacillus spp. (Bacillus subtilis) with the Bacillus piece you formate miss (Bacillus licheniformis) F1017, Bacillus piece you formate miss (Bacillus licheniformis) F1232, Bacillus piece you formate miss (Bacillus licheniformis) F1267, Bacillus piece you formate miss (Bacillus licheniformis Bacillus licheniformis F1200, Bacillus pumilus F1337, Bacillus sonorensis F1005, Bacillus subtilis F1236, and Bacillus subtilis ) F1279 can be used.

The method of producing the fermented extract used in the composition of the present invention is not particularly limited, and a general method known in the art can be used. Specifically, the fermented extract can be obtained by adding the germ to the ginseng, culturing it, and fermenting it.

More specifically, when yeast is used at the time of fermentation, yeast is inoculated to a basic medium containing ginseng powder or extract at a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL), and the pH is 5.5 to 6.5 and the temperature is 25 to 50 ° C , Aeration amount of 0.05 to 0.5 VVM, and then the fermentation broth is centrifuged to obtain a final ginseng fermentation extract in which polymer precipitates and yeast bacteria are removed. The incubation time is preferably 24 to 120 hours.

When the mushroom is used for fermentation, the mushroom is inoculated into the ginseng powder and cultured under the conditions of a temperature of 18 to 25 ° C and a humidity of 60 to 80%, and then cultured in water or an anhydrous or low- It is preferable to use at least one of alcohol (for example, methanol, ethanol, propanol and butanol), a mixed solvent of the lower alcohol and water, acetone, ethyl acetate, chloroform, 1,30 butylene glycol, hexane or diethyl ether , Heated at 50 to 100 ° C for 1 to 5 hours, and concentrated under reduced pressure to obtain the final ginseng fermented extract. The incubation time is preferably 1 to 100 days.

When lactic acid bacteria are used for fermentation, lactic acid bacteria are inoculated into a basic medium containing ginseng powder or extract at a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL) After culturing while maintaining the conditions of 0.5 to 1.5 VVM, the fermentation broth is centrifuged to obtain a final ginseng fermentation extract in which polymer precipitates and lactic acid bacteria are removed. The incubation time is preferably 24 to 120 hours.

When the ginseng is used for fermentation, the ginseng powder or extract is inoculated with ginseng, and the ginseng is cultured while maintaining the temperature at 15 to 45 ° C. The fermentation broth is centrifuged to remove the polymer precipitate and the final ginseng fermentation extract Can be obtained. The incubation time is preferably 18 to 120 hours.

When Bacillus sp. Bacillus is used for fermentation, Bacillus sp. Bacteria are inoculated to a basal medium containing ginseng powder or extract at a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL) and maintained at a temperature of 10 to 60 ° C After culturing, the fermentation broth is centrifuged to obtain a final ginseng fermentation extract having a pH of 5.5 to 8.5, a polymer precipitate and a Bacillus homogenate removed. The incubation time is preferably 24 to 96 hours.

In one embodiment of the present invention, the weight ratio between the extract of the forage-treated ginseng and the extract of the forage-treated green tea may be 0.1 to 10: 1 in the composition for reducing pores, controlling sebum, or alleviating or improving skin troubles. It is possible to maximize the synergy effect of the two components in the weight ratio. From the above viewpoint, the weight ratio between the extract of forage-treated ginseng and the extract of forage-treated green tea may be 0.5 to 9.5: 1, 1 to 9: 1 or 1.5 to 8.5: 1.

In the composition for reducing pores, controlling sebum or alleviating or alleviating skin troubles, which is one aspect of the present invention, the composition comprises the extract of forage-treated ginseng and the extract of forage-treated green tea in an amount of 0.001 to 10 wt% can do. If the content of the extract is less than 0.001% by weight, the effect of improving skin troubles such as pore reduction, sebum control or anti-inflammation, anti-allergy, acne and atopy is insignificant and if it exceeds 10% by weight, It is not. In view of the above, the composition of the present invention comprises 0.005 to 9.5% by weight, 0.01 to 9% by weight, 0.03 to 8.5% by weight of the extract of forage-treated ginseng and the extract of forage- 0.05 to 8% by weight, 0.07 to 7.5% by weight, 0.09 to 7% by weight, 0.1 to 6.5% by weight, 0.3 to 6% by weight, 0.5 to 5.5% by weight or 0.7 to 5% by weight.

In one aspect of the invention, the composition comprises a cosmetic composition.

The cosmetic composition according to the present invention may be provided in all formulations suitable for topical application. For example, it may be provided as a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in water, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol composition. Compositions of such formulations may be prepared according to conventional methods in the art.

The cosmetic composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, so long as they do not impair the main effect. The cosmetic composition according to the present invention may further comprise a moisturizing agent, an emollient agent, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cold agent or a limiting agent. The compounding amount of the above components can be easily selected by those skilled in the art within a range not to impair the objects and effects of the present invention. The amount thereof may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight, have.

In one aspect of the invention, the composition comprises a pharmaceutical composition.

When the composition according to the present invention is applied to medicines, it may be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding an inorganic or organic carrier to the composition as an active ingredient.

Examples of the agent for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. Examples of the parenteral administration agent include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method. Surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffering agents, suspending agents and other adjuvants can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously and the like.

The effective ingredients of the pharmaceutical composition of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be treated, administration route or judgment of the prescriber. Determination of the amount of application based on these factors is within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / / Day, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[Comparative Example 1] Production of ginseng polysaccharide

1 kg of dried white ginseng was pulverized with a mixer, and 10 L of purified water was added thereto, followed by stirring and extraction at 40 ° C in hot water for 24 hours, followed by immersion for one day at 15 ° C. Thereafter, the residue and filtrate are separated by filtration through a filter cloth and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much concentrated ethanol as the concentrated amount was added dropwise, followed by hot air drying to obtain 65 g of ginseng polysaccharide.

[Comparative Example 2] Production of green tea polysaccharide

1 kg of first processed green tea leaves were pulverized with a blender, and 10 L of purified water was added thereto. The mixture was stirred for 24 hours at 40 캜 in hot water, and then immersed at 15 캜 for 1 day. Thereafter, the residue and filtrate are separated by filtration through a filter cloth and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much concentrated ethanol as the concentrated amount was added dropwise, followed by hot air drying to obtain 72 g of a green tea polysaccharide.

[Comparative Example 3] Preparation of ginseng powder extract containing ginseng polysaccharide (red ginseng polysaccharide)

The non-dried ginseng was put into a high-pressure steam boiler and steamed at 97 ° C for about 4 hours under high pressure. Then, the steamed ginseng that had undergone the above process steps was dried at 65 ° C in a hot air dryer, 1 kg of ginseng was pulverized with a blender, and 10 L of purified water was added thereto. The mixture was stirred and extracted with hot water at 40 ° C for 24 hours, and then immersed at 15 ° C for 1 day. Thereafter, the residue and filtrate are separated by filtration through a filter cloth and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much ethanol as the concentrated amount was added by dropwise method, and 70 g of the processed ginseng polysaccharide (red ginseng polysaccharide) was obtained by using hot air blow drying method using the forge processing technique.

[Comparative Example 4] Preparation of green tea fomentate extract containing green tea polysaccharide

The first-processed green tea leaves were put in a high-pressure tiller and heated at a temperature of 97 ° C for about 4 hours under high pressure. The green tea leaves were dried at 65 ° C in a hot-air dryer Then, 1 kg of processed green tea leaves were ground with a blender, and then 10 L of purified water was added thereto. The resulting mixture was stirred for 24 hours at 40 캜 in hot water, and then immersed at 15 캜 for 1 day. Thereafter, the residue and filtrate are separated by filtration through a filter cloth and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much ethanol as the concentrated amount was added by dropwise method, and 70 g of the processed green tea polysaccharide was obtained by using hot air blow drying method using a forge processing technique.

[Comparative Example 5] Preparation of a mixture of ginseng extract and green tea extract

The ginseng polysaccharide and the green tea polysaccharide prepared in Comparative Example 1 and Comparative Example 2 were simply mixed at a ratio of 1: 1.

[Example 1] Preparation of mixed natural fermentation polysaccharide

A mixture of 50% by weight of non-dried ginseng and 50% by weight of non-dried green tea was mixed with 5% by weight of oligosaccharide and placed in a pot. When the pot was filled, the pot was covered with the lid of the pot. Naturally fermented. After the fermentation, the fermented raw material was filtered with a filter cloth to remove the residue. 10 kg of purified water was added to 1 kg of the filtrate, and the mixture was stirred and extracted with hot water at 40 DEG C for 24 hours, and then immersed at 15 DEG C for 1 day. Thereafter, the filtrate and the filtrate are separated by filtration and centrifugation through filter paper, and the separated filtrate is concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much concentrated ethanol as the concentrated amount was added dropwise, followed by hot air drying to obtain 67 g of a mixture of ginseng polysaccharide and green tea polysaccharide (mixed natural fermentation polysaccharide).

[Example 2] Preparation of mixed bacterial fermentation polysaccharide

A mixture of 50% by weight of dried ginseng and 50% by weight of green tea was pulverized and added to 2 L of purified water so as to be 5% by weight to prepare a carbon source, a nitrogen source, a ginseng salt, an inorganic salt and a micronutrient as basic constituents, To an appropriate pH of 5.5 to 6.5. Lactobacillus was inoculated into the culture medium containing the ginseng and green tea mixture at a concentration of 106 cells / mL and cultured for 72 hours under the conditions of pH 6.5 to 7.5, temperature 35 to 38 ° C, aeration amount 1 VVM After the fermentation, the fermented raw materials were filtered with a filter cloth to remove the residues. Several times, 200 g of the fermentation product obtained was repeatedly added with 2 L of purified water, stirred and then extracted with hot water at 40 DEG C for 24 hours, . Thereafter, the filtrate and the filtrate are separated by filtration and centrifugation through filter paper, and the separated filtrate is concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much concentrated ethanol as the concentrated amount was added dropwise, followed by hot air drying to obtain 12 g of a mixture of ginseng polysaccharide and green tea polysaccharide (mixed bacterial fermentation polysaccharide).

[Example 3] Preparation of mixed salted-fermented polysaccharide

50% by weight of unscented ginseng and 50% by weight of non-dried green tea and an appropriate amount of flame retardant are added to the pot and covered with agar for 7 days to adequately cover the ginseng. After that, it is simply washed and then heated in a high pressure steam sterilizer at 97 ° C for 3 to 24 hours to prepare red ginseng and green tea mixture. And then dried in a hot air dryer at 60 ° C for 24 hours. Then, 10% by weight of yeast and 2% of salt (charcoal) water to cover the red ginseng and green tea mixture are added to the weight of the dried red ginseng and green tea mixture, and fermentation is performed for 5 days. After the fermentation, the fermented raw material was filtered with a filter cloth to remove the residue. 10 kg of purified water was added to 1 kg of the filtrate, and the mixture was stirred and extracted with hot water at 40 DEG C for 24 hours, and then immersed at 15 DEG C for 1 day. Thereafter, the filtrate and the filtrate are separated by filtration and centrifugation through filter paper, and the separated filtrate is concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much concentrated ethanol as the concentrated amount was added dropwise, and the mixture was hot-air dried to obtain 67 g of a mixture of ginseng polysaccharide and green tea polysaccharide (mixed salted and fermented polysaccharide).

[Test Example 1] Inflammation improving effect

1. Inhibitory effect of prostaglandins

The anti - inflammatory effect was evaluated by the inhibitory effect of prostaglandin production. The effects were measured on macrophages using the above-described Comparative Examples 1 to 5 and Examples 1 to 3. First, aspirin was added to the macrophage collected from mouse peritoneum to a final concentration of 500 uM to irreversibly inhibit the cyclooxygenase (COX) activity remaining in the cells. Then, the suspension was added to each well of a 96-well cell culture tube in an amount of 100 μl, followed by culturing in an incubator of 5% CO ₂ and 37 ° C for 2 hours to adhere macrophages to the surface of the container. Then, the adhered macrophages were washed three times with PBS and used for the inflammation improving effect test. RPMI medium supplemented with 1% (w / v) LPS was added to the cultured macrophage 5x10 ⁴ cells / ml for 12 hours to induce the production of prostaglandin. Polysaccharide and green tea polysaccharide was treated with 100 μl of the mixture, and the liberated prostaglandin was quantitated by enzyme immunoassay (ELISA).

At this time, the inhibitory activity on the production of prostaglandin in the mixture of the ginseng polysaccharide and the green tea polysaccharide processed by using the forage, which is the herbal processing technique of Example 1, was found to be 100% in the case of the prostaglandins produced in the LPS- The results were compared with those of the control group by the percentage of prostaglandin reduced by treating the LPS and the sample together. As a result, the inhibitory effect of prostaglandin production is shown in Table 1 below.

Test substance Prostaglandin inhibition (%) NT (Blank) 0% Control group (aspirin treatment group) 58.6% Comparative Example 1 20.2% Comparative Example 2 45.5% Comparative Example 3 22.3% Comparative Example 4 46.9% Comparative Example 5 33.8% Example 1 56.5% Example 2 56.8% Example 3 55.1%

As shown in Table 1, in the case of Examples 1 to 3 of the present invention, the effect of inhibiting the production of prostaglandins is remarkably high as in the control group treated with aspirin. In particular, it can be confirmed that the prostaglandin production inhibitory effect is remarkably higher than that of the comparative example not subjected to fog treatment.

Therefore, it can be seen that the composition of the present invention can provide an excellent inflammation-improving effect. In addition, it can be seen that the composition of the present invention can prevent and improve skin troubles by inhibiting the expression of prostaglandin, which is a skin inflammation factor.

2. IL-8 production inhibitory effect

Before the experiment, the skin keratinocyte (NHEK, available from Lonza) was dispensed into a 96-well plate at 5 × 10 ⁴ cells / well and incubated at 37 ° C in a 5% CO ₂ incubator incubator) for 24 hours. Twenty-four hours later, the cells were washed twice with PBS and replaced with serum free keratinocyte basement media (KBM). A mixture of the ginseng polysaccharide and the green tea polysaccharide processed in the respective wells using the forages of Comparative Examples 1 to 5 and Examples 1 to 3 was treated for each concentration shown in Table 2 below and reacted for 30 minutes. 10 mu g / ml) was treated in each well. Here, peptidoglycan from S. aureus (PGSA ) is a peptidoglycan extracted from Staphylococcus aureus, which is a major constituent of the cell wall of Gram-positive (+) bacteria, and the cell membrane components of bacteria are known to cause inflammation, Especially in staphylococcus, about 90% of atopic patients are reported to cause secondary infection by this bacterium. After culturing in a 5% CO ₂ incubator at 37 ° C for 24 hours, the culture was taken and ELISA for interleukin-8 (IL-8) was performed. The results are shown in Table 2 below. The ELISA used the experimental method of BD science.

Test substance IL-8 secretion (pg / ml) Untreated Control (Control) 755.44 PGSA (10 [mu] g / ml) 4215.46 Comparative Example 1 (50 ppm) + PGSA (10 占 퐂 / ml) 3905.21 Comparative Example 2 (50 ppm) + PGSA (10 占 퐂 / ml) 2975.94 Comparative Example 3 (25 ppm) + PGSA (10 占 퐂 / ml) 3552.40 Comparative Example 3 (50 ppm) + PGSA (10 占 퐂 / ml) 2943.38 Comparative Example 4 (25 ppm) + PGSA (10 占 퐂 / ml) 3319.48 Comparative Example 4 (50 ppm) + PGSA (10 占 퐂 / ml) 2879.92 Comparative Example 5 (50 ppm) + PGSA (10 占 퐂 / ml) 3492.54 Example 1 (25 ppm) + PGSA (10 占 퐂 / ml) 2611.31 Example 1 (50 ppm) + PGSA (10 占 퐂 / ml) 1926.72 Example 2 (25 ppm) + PGSA (10 占 퐂 / ml) 2590.40 Example 2 (50 ppm) + PGSA (10 占 퐂 / ml) 1952.18 Example 3 (25 ppm) + PGSA (10 占 퐂 / ml) 2679.66 Example 3 (50 ppm) + PGSA (10 占 퐂 / ml) 1997.28

As shown in Table 2, it was confirmed that the composition of the present invention significantly reduced or suppressed the secretion of IL-8 increased by PGSA and LPS. In particular, it was confirmed that IL-8 secretion inhibitory effect was remarkably higher than that of Comparative Example not subjected to Foaming treatment.

Thus, it can be seen that the composition of the present invention significantly reduces the secretion of IL-8 by PGSA, thereby providing excellent anti-inflammatory effects.

[Test Example 2] Evaluation of itching relief

At the end of the experiment, keratinocytes (cell line name: HaCaT, available from ATCC) were dispensed in a 96 well plate at ⁴ x ¹⁰ cells / well and incubated at 37 ° C in a 5% CO ₂ incubator And cultured for 24 hours. After 24 hours, the 96-well plate was washed twice with HBSS (Hanks' Balanced Salt Solution) buffer, and the reaction buffer (2 μM Fluo-4-AM, 20% pluronic acid, 2.5 mM probenecid) gave. After incubation at 37 ° C in a 5% CO ₂ incubator for 30 minutes and at room temperature for 30 minutes, cells were washed twice with HBSS buffer and treated with cells of Examples 1 to 3 at the concentrations shown in Table 3 below.

After 10 minutes of reaction, the cells were treated with 2 U / ml trypsin or 5 μM PAR-2 active peptide (SLIGKV) and the change in intracellular Ca ²⁺ concentration was measured for 80 seconds. Measurement of intracellular Ca ²⁺ concentration was performed using FlexStation 3 (Molecular Device, USA). The treated ginseng polysaccharide and green tea polysaccharide mixture were treated with 2U / ml trypsin or 5μM PAR-2 active peptide (SLIGKV) using forage processing technology of Comparative Examples 1 to 5 and Examples 1 to 3 for 80 seconds The difference between the minimum value and the maximum value of the value obtained by measuring the flex was measured and compared with the difference between the minimum value and the maximum value when 2U / ml trypsin or 5μM PAR-2 active peptide (SLIGKV) The percent inhibition of intracellular entry of calcium ions is shown in Table 3 below.

Test substance concentration Inhibition rate of intracellular influx of calcium ions (%) Trypsin (2 U / ml) PAR-2 active peptide (5 [mu] M) Comparative Example 1 0.1% 14 17 0.5% 16 19 Comparative Example 2 0.1% 19 22 0.5% 22 27 Comparative Example 3 0.1% 14 17 0.5% 17 20 Comparative Example 4 0.1% 20 23 0.5% 24 28 Comparative Example 5 0.1% 16 19 0.5% 19 23 Example 1 0.05% 25 27 0.1% 28 30 0.5% 35 39 Example 2 0.05% 27 29 0.1% 29 32 0.5% 37 42 Example 3 0.05% 24 26 0.1% 28 30 0.5% 36 40

As shown in Table 3, the composition of the present invention reduces the influx of intracellular calcium ions by trypsin or PAR-2 activating peptide (SLIGKV) and increases intracellular calcium ions remarkably Respectively. Particularly, the composition of the present invention is able to confirm the remarkable effect of inhibiting calcium ion inflow as compared with the comparative example not subjected to foaming treatment.

Therefore, the composition of the present invention can effectively inhibit the itching-inducing PAR-2 activity, thereby providing a superior antinociceptive effect.

[Test Example 5] Test for antimicrobial activity against acne bacteria

Formulation Examples 1 to 6 and Comparative Formulation Examples 1 to 7 were prepared according to the components and the contents (% by weight) shown in Tables 4 and 5 below. Specifically, Formulation Examples 1 to 6 were obtained by mixing a mixture of fodder-treated ginseng and green tea extract, Comparative Formulation Example 1 contained no effective ingredient for improving acne skin, Comparative Formulation Example 2 had antibacterial activity As a standard, erythromycin (erythromycin), which is widely used as an acne remedy, is included as a standard substance.

The manufacturing methods of Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 7 are as follows. The components of A in the following Tables 4, 5 and 6 were completely dissolved, the components of the B phase were completely dissolved in a separate melting tank, and the B phase was added to the A phase to be mixed and solubilized. The ingredients of the C phase were added thereto in accordance with the mixing ratios shown in Tables 4 and 5, mixed and homogenized, and then filtered to prepare the compositions.

The unit of the content ratio of the following ingredients is% by weight.

Compounding ingredient
(weight%) Formulation Example 1 Formulation Example 2 Formulation Example 3 Comparative Formulation Example 1 Comparative Formulation Example 2 A Purified water To 100 To 100 To 100 To 100 To 100 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 glycerin 5.0 5.0 5.0 5.0 5.0 B ethanol 2.0 2.0 2.0 2.0 2.0 PEG-60 hardened castor oil
(hydrogenated castor oil) 0.4 0.4 0.4 0.4 0.4 Perfume 0.04 0.04 0.04 0.04 0.04 C Example 1 5.0 - - - - Example 2 - 5.0 - - - Example 3 - - 5.0 - - Erythromycin - - - - 5.0

Fold composition
(weight%) Comparative Formulation Example
3 Comparative Formulation Example
4 Comparative Formulation Example
5 Comparative Formulation Example
6 Comparative Formulation Example
7 A Purified water To 100 To 100 To 100 To 100 To 100 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 glycerin 5.0 5.0 5.0 5.0 5.0 B ethanol 2.0 2.0 2.0 2.0 2.0 PEG-60 hardened castor oil
(hydrogenated castor oil) 0.4 0.4 0.4 0.4 0.4 Perfume 0.04 0.04 0.04 0.04 0.04 C Comparative Example 1 5.0 - - - - Comparative Example 2 - 5.0 - - - Comparative Example 3 - - 5.0 - - Comparative Example 4 - - - 5.0 - Comparative Example 5 - - - - 5.0

Each of the cosmetic compositions prepared in the formulations of Formulation Examples 1 to 3 and Comparative Formulations 1 to 7 was tested for antibacterial activity against Propionibacterium acnes (ATCC 6919: medium-BHI broth), which is a cause of acne Respectively.

The test method for the antimicrobial activity against acne bacteria is as follows.

(1) Preparation of test strain

Propionibacterium acnes were cultured in an anaerobic culture inoculated on BHI broth.

(2) Dilution solution preparation

0.15 ml of the above test solution was added to 15 ml of BHI broth (pH 6.8) or LB broth (pH 4.5), and the mixture was used as a dilute solution.

(3) Preparation of sample

Each of the compositions prepared from Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 7 was used as a sample.

(4) Antimicrobial activity test

1) In a 96-well 96-well plate, place the sample in line 1 and add 200 μl of diluted solution.

2) Thoroughly mix the mixture of line 1, and then take 100 μl of the mixture, place it in the second row, mix well, and then repeat the double dilution by adding 100 μl to the third row.

3) After culturing the cells at 32 ° C for 24 hours and 48 hours, determine whether the bacteria are proliferating to the extent of suspension, and determine the minimum concentration without bacterial growth as the MIC (Minimum Inhibitory Concentration) value. If the mixture is opaque and it is difficult to judge the growth of the microorganism, check with a microscope.

The results of the antimicrobial activity test against acne bacteria are shown in Table 6 below. The MIC was expressed in terms of the concentration of the active ingredient contained in the formulation.

Item pH Propionibacterium acnes Formulation Example 1 5.7 > 49 ppm Formulation Example 2 5.7 > 50 ppm Formulation Example 3 5.7 > 49 ppm Comparative Formulation Example 1 5.7 Maximum concentration (no antimicrobial activity) Comparative Formulation Example 2 5.7 > 82 ppm Comparative Formulation Example 3 5.7 > 155 ppm Comparative Formulation Example 4 5.7 > 100 ppm Comparative Formulation Example 5 5.7 > 147 ppm Comparative Formulation Example 6 5.7 > 100 ppm Comparative Formulation Example 7 5.7 > 129 ppm

The lower the ppm concentration in the MIC, the more effective the antimicrobial activity against acne bacteria. In the case of Formulation Examples 1 to 3 of the present invention, it was confirmed that they have very excellent antibacterial activity because they have a significantly lower ppm concentration than Comparative Formulation Example 2 using erythromycin, which is a known acne treatment agent. It was also confirmed that Formulation Examples 1 to 3 of the present invention had significantly higher antibacterial effects than Comparative Formulation Examples 3 to 7.

[Test Example 6] Test for improving atopy symptoms in human body

The cream containing the forage-treated ginseng extract and the forage-treated green tea extract was prepared with the compositions shown in Table 7 and Table 8 below.

Compounding ingredient
(weight%) Formulation Example 4 Formulation Example 5 Formulation Example 6 Purified water To 100 To 100 To 100 Example 1 1.0 - - Example 2 - 1.0 - Example 3 - - 1.0 Vegetable hydrogenated oil 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 Stearyl alcohol 2.00 2.00 2.00 Glycerol stearate 1.00 1.00 1.00 Polyglyceryl-10pentastearate and behenyl alcohol and sodium stearoyl lactylate 1.00 1.00 1.00 Arachidyl behenyl alcohol and arachidyl glucoside 1.00 1.00 1.00 Cetylaryl Alcohol and Cetearyl Glucoside 2.00 2.00 2.00 PEG-100 Stearate & Glycerololate & Propylene Glycol 1.50 1.50 1.50 Caprylic / capric triglyceride 11.00 11.00 11.00 Cyclomedicone 6.00 6.00 6.00 Triethanolamine 0.1 0.1 0.1

Compounding ingredient
(weight%) Comparative Formulation Example
8 Comparative Formulation Example
9 Comparative Formulation Example
10 Comparative Formulation Example
11 Comparative Formulation Example
12 Comparative Formulation Example
13 Purified water To 100 To 100 To 100 To 100 To 100 To 100 Comparative Example 1 - 1.0 - - - - Comparative Example 2 - - 1.0 - - - Comparative Example 3 - - - 1.0 - - Comparative Example 4 - - - - 1.0 - Comparative Example 5 - - - - - 1.0 Vegetable hydrogenated oil 1.50 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 2.00 Glycerol stearate 1.00 1.00 1.00 1.00 1.00 1.00 Polyglyceryl-10pentastearate and behenyl alcohol and sodium stearoyl lactylate 1.00 1.00 1.00 1.00 1.00 1.00 Arachidyl behenyl alcohol and arachidyl glucoside 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol and Cetearyl Glucoside 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 Stearate & Glycerololate & Propylene Glycol 1.50 1.50 1.50 1.50 1.50 1.50 Caprylic / capric triglyceride 11.00 11.00 11.00 11.00 11.00 11.00 Cyclomedicone 6.00 6.00 6.00 6.00 6.00 6.00 Triethanolamine 0.1 0.1 0.1 0.1 0.1 0.1

The creams of Formulations 4 to 6 and Comparative Formulations 8 to 13 prepared with the compositions of Table 8 and Table 9 were applied to the panels to assess the degree of improvement of atopic dermatitis. For each panel of 90 panelists who thought that there was a symptom of dermatitis showing similar symptoms, each composition was divided into 9 groups of 10 each, and each composition of Tables 7 and 8 was administered twice daily at a temperature of 24 to 26 DEG C and a humidity of 75% And then applied to the face for 8 weeks. At the end of the test, subjects were asked to fill out a questionnaire and conducted a subjective efficacy evaluation. In the questionnaire, symptoms of atopic dermatitis were classified as "pruritus", "dryness", "keratinization", "dandruff", "erythema", "swelling", "skin crack", " And the degree of improvement was evaluated for each symptom in the case of the above symptoms, and the improvement effect of the atopy symptom was evaluated on an individual basis. The results are shown in Table 9.

Application group (number of respondents - persons) Significant improvement Improving No change worse Formulation Example 4 4 5 One 0 Formulation Example 5 4 4 2 0 Formulation Example 6 3 6 One 0 Comparative Formulation Example 8 0 5 5 0 Comparative Formulation Example 9 0 6 4 0 Comparative Formulation Example 10 One 7 3 0 Comparative Formulation Example 11 One 6 3 0 Comparative Formulation Example 12 One 7 3 0 Comparative Formulation Example 13 One 6 3 0

As a result, it was confirmed that the composition of the present invention had a more excellent effect for improving atopic dermatitis symptoms than the Comparative Example 8. Furthermore, it was confirmed that the composition of the present invention had a superior atopic dermatitis improving effect than Comparative Formulation Examples 9 to 13 containing Comparative Examples 1 to 5.

[Test Example 7] Effect of suppressing sebum secretion

To evaluate sebum secretion inhibiting effects of the above Examples and Comparative Examples, the following evaluation was made. Seventy two male and female subjects who felt that sebum secretion was high were selected and divided into 9 groups of 8 groups each, and the creams of Formulation Examples 4 to 6 and Comparative Formulation Examples 8 to 13 prepared in the compositions of Tables 7 and 8 7-8] for four weeks. The determination of the effect of sebum reduction was made using a sebum analyzer and the results are shown in Table 10 below.

Subject Reduction rate of sebum after 2 weeks (%) Reduction rate of sebum after 4 weeks (%) Formulation Example 4 15.4 ± 2.8% 17.6 + 2.4% Formulation Example 5 15.0 + 2.9% 17.2 ± 2.9% Formulation Example 6 14.9 ± 3.1% 17.0 + 3.0% Comparative Formulation Example 8 5.1 ± 1.9% 6.5 ± 1.8% Comparative Formulation Example 9 5.2 ± 1.8% 6.5% ± 2.0 Comparative Formulation Example 10 5.4 ± 1.8% 6.6 ± 2.1% Comparative Formulation Example 11 5.3 ± 2.0% 6.4 ± 1.8% Comparative Formulation Example 12 5.2 ± 1.8% 6.3 ± 1.7% Comparative Formulation Example 13 5.2 ± 1.5% 6.5 ± 2.0%

From the results shown in the above Table 10, Formulation Examples 4 to 6 containing the green tea ginseng polysaccharide obtained by the fermentation method according to the present invention as the active ingredient effectively inhibited excess sebum secreted from Comparative Formulations 8 to 13, .

Therefore, the composition for external application for skin containing green tea ginseng polysaccharide obtained by the fermentation method according to the present invention has an excellent sebum secretion inhibiting effect.

[ Test Example  8] Pore reduction effect test

In order to examine the effect of reducing pores in the above-mentioned Examples and Comparative Examples, the following evaluation was made. Seventy-two subjects, male and female, having a wide pore size were selected and divided into nine groups of eight each, and the creams of Formulation Examples 4 to 6 and Comparative Formulation Examples 8 to 13 prepared in the compositions of Tables 7 and 8 were applied to the face for four weeks I did it right. The judgment of the effect of pore reduction was made by experts after taking photographs before and 4 weeks after the experiment. The results are shown in Table 11 below. (Rating: 0. not reduced at all - 5. very shrunk)

matter Rating Rating Formulation Example 4 3.1 Formulation Example 5 3.0 Formulation Example 6 3.0 Comparative Formulation Example 8 0.9 Comparative Formulation Example 9 0.8 Comparative Formulation Example 10 0.8 Comparative Formulation Example 11 1.0 Comparative Formulation Example 12 0.9 Comparative Formulation Example 13 1.0

From the results shown in the above Table 11, Formulation Examples 4 to 6 containing the green tea ginseng polysaccharide obtained by the fermentation method according to the present invention as an active ingredient had a better effect of reducing the pore size than the Comparative Forms Examples 8 to 13 which did not contain them And it was found.

[ Test Example  9] Sensory Evaluation of Pore Reduction Effect

Lotion containing Foaming Ginseng Extract and Foaming Green Tea Extract was prepared with the compositions shown in Table 12 below.

Compounding ingredient
(weight %) Formulation Example 4 Formulation Example 5 Formulation Example 6 Comparative Formulation Example 14 Comparative Formulation Example 15 Comparative Formulation Example 16 Comparative Formulation Example 17 Comparative Formulation Example 18 Comparative Formulation Example 19 Example 1 0.2 - - - - - - - - Example 2 - 0.2 - - - - - - - Example 3 - - 0.2 - - - - - - Comparative Example 1 - - - - 0.2 - - - - Comparative Example 2 - - - - - 0.2 - - - Comparative Example 3 - - - - - - 0.2 - - Comparative Example 4 - - - - - - - 0.2 - Comparative Example 5 - - - - - - - - 0.2 glycerin 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Butylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Propylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Carboxyvinyl polymer 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Phage-12 nonylphenyl ether 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Polysorbate 80 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 ethanol 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 Triethanolamine 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Preservative, coloring, fragrance Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Purified water Balance Balance Balance Balance Balance Balance Balance Balance Balance

90 of the women aged 25 to 35 years were divided into nine groups of 10 each for each group, and the formulations 4 to 6 and the comparative formulations 14 to 19 of Table 12 were applied to the lotion The composition was used, and the degree of skin improvement was judged by self-measurement according to the following evaluation criteria. The results are shown in Table 13 below.

<Evaluation Criteria>

+++: Very good improvement.

++: Significant improvement.

+: There is a slight improvement.

±: No improvement, but no deterioration.

-: No improvement, but rather worse.

Subject 1 week 2 weeks 3 weeks 4 weeks Formulation Example 4 + ++ ++ +++ Formulation Example 5 + ++ ++ +++ Formulation Example 6 ± + ++ +++ Comparative Formulation Example 13 ± ± + + Comparative Formulation Example 14 ± ± ± ± Comparative Formulation Example 15 ± ± ± ± Comparative Formulation Example 16 ± ± + + Comparative Formulation Example 17 ± ± ± ± Comparative Formulation Example 18 ± ± + +

As shown in Table 13, Formulations (4 to 6) comprising green tea ginseng extract obtained by one or more fermentation methods according to the present invention are superior to Comparative Formulations (14 to 19) Reduction effect. Therefore, the composition for external application for skin containing the forage-treated green tea ginseng extract according to the present invention has excellent pore reduction effect. In addition, since sebum is secreted from the pores, the effect of suppressing secretion of sebum is also exhibited as the pores are reduced.

Hereinafter, a formulation example of the composition according to the present invention will be described. However, the pharmaceutical composition and the cosmetic composition can be applied to various formulations, which are not intended to limit the present invention but merely to illustrate the present invention.

[Formulation Example 1] Soft capsule

400 mg / capsule was prepared by mixing 150 mg of the ginseng extract of Foge treatment, 150 mg of the green tea extract of foge treatment, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow radish and 6 mg of lecithin. .

[Formulation Example 2] Tablets

The granules were prepared by mixing 150 mg of fosse treated ginseng extract, 150 mg of forage-treated green tea extract, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate and 40 mg of 30% ethanol, Lt; 0 &gt; C and tableted by tabletting machine.

[Formulation Example 3] Granules

The granules were prepared by mixing 150 mg of Fosse-treated ginseng extract, 150 mg of forage-treated green tea extract, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch and 100 mg of 30% ethanol, followed by drying at 60 ° C. And then filled in the bubbles. The final weight of the contents was 1 g.

[Formulation Example 1] Softening lotion (skin lotion)

The softening longevity was prepared by a conventional method according to the composition shown in Table 14 below.

Compounding ingredient Content (% by weight) Foam treated ginseng and green tea extract (ginseng: green tea = 1: 1) 0.2 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 2] Nutritional lotion (Milk lotion)

Nutrition lotion was prepared according to a conventional method according to the composition shown in Table 15 below.

Compounding ingredient Content (% by weight) Foam treated ginseng and green tea extract (ginseng: green tea = 1: 1) 1.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capric triglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Liquid paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 3] Nourishing cream

Nutrition creams were prepared according to the compositions shown in Table 16 below in a conventional manner.

Compounding ingredient Content (% by weight) Foam treated ginseng and green tea extract (ginseng: green tea = 1: 1) 2.0 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 4] Massage cream

Massage creams were prepared in a conventional manner according to the compositions shown in Table 17 below.

Compounding ingredient Content (% by weight) Foam treated ginseng and green tea extract (ginseng: green tea = 1: 1) 3.0 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 5] Pack

Packs were prepared in a conventional manner according to the composition shown in Table 18 below.

Compounding ingredient Content (% by weight) Foam treated ginseng and green tea extract (ginseng: green tea = 1: 1) 0.2 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 Good Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 6] ointment

Ointments were prepared in a conventional manner according to the composition shown in Table 19 below.

Compounding ingredient Content (% by weight) Foam treated ginseng and green tea extract (ginseng: green tea = 1: 1) 0.2 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservative, coloring, fragrance Suitable amount Purified water Balance

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (16)

A mixture of ginseng natural fermentation polysaccharide and green tea natural fermentation polysaccharide; A mixture of a ginseng fermentation polysaccharide and a green tea fermentation polysaccharide; And a mixture of ginseng salted-fermentation polysaccharide and green tea salted-fermentation polysaccharide, and a mixture of a forage-treated ginseng extract and a forge-treated green tea extract as an active ingredient. The method according to claim 1,
Wherein said skin disorder is at least one of inflammation, allergy, atopy, bacterial infection and acne. 3. The method of claim 2,
Wherein the bacterium is an acne bacterium, for alleviating or improving skin troubles. The composition according to claim 1, wherein the composition inhibits or inhibits the production of prostaglandins. The composition according to claim 1, wherein the composition inhibits or inhibits the production of interleukin. The composition of claim 1, wherein the weight ratio of the ginseng polysaccharide to the green tea polysaccharide is 0.1 to 10: 1. 2. The composition of claim 1, wherein the composition comprises ginseng polysaccharide and green tea polysaccharide in an amount of 0.001 to 10 weight%, respectively, based on the total weight of the composition. A mixture of ginseng natural fermentation polysaccharide and green tea natural fermentation polysaccharide; A mixture of a ginseng fermentation polysaccharide and a green tea fermentation polysaccharide; And a mixture of ginseng salted-fermentation polysaccharide and green tea salted-fermentation polysaccharide, and a mixture of a forge-treated ginseng extract and a forge-treated green tea extract as an active ingredient. 9. The composition of claim 8, wherein the weight ratio of the ginseng polysaccharide to the green tea polysaccharide is 0.1 to 10: 1. 9. The composition of claim 8, wherein the composition comprises ginseng polysaccharide and green tea polysaccharide in an amount of 0.001 to 10 wt%, respectively, based on the total weight of the composition. 11. The method according to any one of claims 1 to 10,
Wherein the fermentation is carried out with at least one bacterium selected from the group consisting of yeast, mushroom, lactic acid, mycelia, and Bacillus. 12. The method of claim 11,
The natural fermentation polysaccharide is obtained by mixing ginseng, green tea or a mixture thereof with an oligosaccharide; Spontaneously fermenting the mixture for 3 to 9 months in Onggi; Removing the impurities and extracting the impurities; And concentrating and drying the extract. 12. The method of claim 11,
The fermentation polysaccharide may be added to ginseng, green tea or a mixture thereof; Inoculating at least one bacterium selected from the group consisting of yeast, mushroom, lactic acid, mycelia, and Bacillus subtilis; Fermenting for 10 to 100 days; Removing the impurities and extracting the impurities; And concentrating and drying the extract. 12. The method of claim 11,
Salting-fermenting polysaccharides are prepared by aging ginseng, green tea or a mixture thereof with salt; Heating and drying; Treating the brine; Fermenting for 3 to 7 days; Removing the impurities and extracting the impurities; And concentrating and drying the extract. 11. The composition according to any one of claims 1 to 10, wherein the composition is a cosmetic composition. 11. The composition according to any one of claims 1 to 10, wherein the composition is a pharmaceutical composition.