KR102027453B1 - Composition containing extract using process of herbal medicine - Google Patents

Abstract

The present invention relates to a composition comprising a foam extract of ginseng and a foam extract of green tea.

Description

COMPOSITION CONTAINING EXTRACT USING PROCESS OF HERBAL MEDICINE}

The present invention relates to a composition comprising an extract of foamed ginseng and an extract of foamed green tea.

The appearance of the skin is responsible for the extracelluar matrix (ECM) component of the dermal tissue, which is collagen, which accounts for about 70-80% of the total ECM. The production of wrinkles on the skin is due to the decreased production or destruction of collagen caused by aging or ultraviolet rays. In particular, due to the expression of matrix metallo protease, such as collagenase (collagenase) collagen normally generated in the skin is broken down to produce wrinkles.

Human skin is the body's primary protective barrier, which protects the body's organs from changes in temperature and humidity, and from external environmental stimuli such as ultraviolet rays and pollutants. Undergo a change. That is, internally, the secretion of various hormones that regulate metabolism decreases, and the function of immune cells and the activity of cells decreases, thereby reducing the biosynthesis of immune proteins and constituent proteins necessary for living organisms. Due to the increase in the amount of ultraviolet rays that reach the surface of the sun's rays and intensifying environmental pollution, free radicals and free radicals increase, thereby reducing the thickness of the skin, increasing wrinkles, reducing elasticity, The color of the skin becomes dull, skin problems frequently occur, and there are various changes such as blemishes, freckles, and black mushrooms.

In order to prevent changes in the skin condition caused by these internal and external factors and to maintain a healthy skin condition, the skin condition is improved by adding bioactive substances obtained from various known animals, plants, and microorganisms to cosmetics. Efforts have been made.

The present inventors have confirmed that the mixture of the ginseng extract of ginseng and the extract of green tea can improve skin troubles, and in particular, has a very good synergy effect than the mixture of common ginseng and green tea extract.

Dermatology therapy, 16, 357-364, 1998

It is an object of the present invention to provide a composition comprising an extract of natural sieves.

In order to achieve the above object, the present invention provides a composition for pore reduction, sebum control or skin trouble relief or improvement comprising a mixture of foamed ginseng extract and foamed green tea extract as an active ingredient.

The composition of the present invention includes a mixture of foamed ginseng extract and foamed green tea extract, and has a remarkably superior effect of alleviating or improving skin troubles than the mixture of each extract not treated or each extract treated with foam. Specifically, the composition of the present invention has a synergistic effect by the mixing of the respective siege treatment extract has a superior anti-inflammatory and anti-allergic effect than the mixture of ordinary ginseng extract and green tea extract, and improves acne and skin troubles, atopic symptoms Can provide. It can also provide the effect of pore shrinkage or sebum control. The compositions of the present invention may also inhibit the expression of prostaglandins, inhibit the production of interleukin and thus alleviate skin itch. In addition, the composition of the present invention has antimicrobial activity and may improve atopic symptoms. In addition, the composition of the present invention can reduce the pores and control the amount of sebum produced in the sebaceous glands and discharged to the skin through the pores, and can alleviate or improve the disease or trouble caused by sebum secretion. Compositions of the present invention, including natural extracts, have little toxicity and irritation and have little side effects even when used for a long time.

Therefore, the composition of the present invention may contain antifoam treatment extracts to provide an overall skin condition improvement effect through anti-inflammatory and anti-allergic effects, pore reduction or sebum control, and skin trouble improvement effects such as acne and atopy.

The present invention relates to a composition for pore reduction or sebum control or skin trouble alleviation or improvement comprising a mixture of foamed ginseng extract and foamed green tea extract as an active ingredient in one aspect.

In one aspect of the present invention, the mixture of foamed ginseng extract and foamed green tea extract is not only a mixed extract obtained by mixing ginseng and green tea, but also each of the ginseng extract and green tea extract obtained by separately extracting ginseng and green tea. It also includes a mixture.

In the present specification, "foam" (匍 制) refers to a pharmaceutical technology that changes the original properties of the medicine by processing the medicine based on the herbal theory. The foam may include a process of boiling, steaming, roasting, roasting or roasting the medicine to reduce the toxicity of the medicine, to preserve the medicine's weakness, to enhance the drug's convenience, or to increase the convenience of taking the medicine. In addition, the foaming method is, for example, the method of roasting medicinal herbs, the method of roasting the medicinal herbs with a certain amount of liquid auxiliary ingredients so that the auxiliary material soaks into the drug tissue, the liquid according to the formulation rules of each item Add auxiliary ingredients, mix and heat them in a suitable container, or steam them until they reach a certain degree. The sanding method is further subdivided according to the intensity of fire and the amount of material used. Some examples are as follows. The neat (淸 炒) method is a method of stir-frying medicinal herbs until the level specified by culture (文火: fire with a weak flame) or fig (武火: fire with a strong flame) is reached. Runner (酒 炙) method is to brew the medicine using the alcohol of about 15%, the jujeok (酒蒸) method is to steam the medicine using the alcohol of about 15%. Even with the same medicinal plants, raw materials and ripened materials may not have the same properties or may differ in function. In addition, a combination of raw materials and ripening materials can maximize the therapeutic effect by elevating the effects of each medicine or offsetting toxicity.

In the present specification, "foam treatment" means pre-treatment of green tea or ginseng by "foam" as defined above, and specifically, but not limited to using the steaming method and porcelain method of the foaming method. The above preparation can be carried out without limitation. Specifically, in the present specification, "foaming treatment" is a processing step comprising the step of applying a) high temperature and high pressure to green tea or ginseng; And b) may be prepared by a process comprising the step of drying the processed green tea or ginseng, but is not limited thereto, and can be used if the formulation is generally used in the art.

In the present specification, "salt (鹽 炙)" refers to a method of roasting with a certain amount of salt water in the medicinal herbs or with the treatment, for example, soaking the medicinal herbs in salt water and then roasting and roasting the medicinal herbs to a certain degree Spraying brine may include a method of roasting again, but is not limited thereto. Salt may include, but is not limited to, sun salt, rock salt, recontaminated salt, purified salt or bamboo salt, and generally includes a kind of salts available.

In the present specification, "chotan" (해당) corresponds to one of the roasting method of the medicinal herbs, for example, but not limited to roasting the outer surface of the medicinal herbs to black, the inside is brown or dark tan.

In the present specification, "choja (醋 炙)" means a method of roasting with a certain amount of vinegar after the treatment or with the treatment, for example, mixed with a certain amount of vinegar, called a method and then roasted in a container After roasting and roasting in a container, if the surface has a glossy or disgusting smell, the method includes roasting by spraying a certain amount of seaweed, but not limited thereto. The vinegar may include, but is not limited to, brewed vinegar, grain vinegar, fruit vinegar, or synthetic vinegar, and includes a kind of vinegar generally available.

In the present specification, "jujum" (酒蒸) means a method of steaming the medicine with a certain amount of alcohol, for example, but including, but not limited to a method of steaming in a steamer after mixing the medicine with a certain amount of sulfur. Alcoholic beverages include, but are not limited to, brewed liquor, distilled liquor, mixed liquor, yellow liquor, sake.

"Gangja" in the present specification means a method of treating the medicine by a certain amount of ginger juice or after roasting or boiling with the treatment, for example, mixed with a certain amount of ginger juice is called and then in a container How to roast and slice the ginger, and then medicinal herbs, and then boiled, and the ginger-tang is completely absorbed, including the method of taking out and drying, but is not limited thereto. Ginger contains the kinds of ginger that are commonly available.

In the present specification, "milch" means a method of roasting the medicine after a certain amount of honey or with the treatment, for example, mixed with a certain amount of softness and left to stand, then honey is tissue When soaked in, including the method of roasting in the container and the medicinal herbs in the container and stir-fry a certain amount of softness and stir stirring quickly, but is not limited thereto. Mildness is honey boiled over low heat to make it dry. Honey includes, but is not limited to, acacia honey, rapeseed honey, and native honey.

"Ginseng" (Panax ginseng C.A. Meyer) is a plant belonging to the genus Ogapi ginseng. In the present specification, the ginseng may be used without limitation in its type and annual root, and ginseng that is dried or not dried may be used. In addition, in the present specification, the ginseng can be used without limitation in the site, specifically, the root can be used.

In the present specification, "green tea" may also be used without limitation in the type and number of days of cultivation, and may use both dried and undried green tea. In addition, in the present specification, the ginseng may be used without limitation in its portion, and specifically, the leaves of green tea may be used.

In the present specification, "skin trouble" is intended to encompass a case where the skin is not in a normal state due to various causes. Specifically, but not limited to, including acne, atopic dermatitis, rash or allergies, and includes swelling or popped skin.

As used herein, the term "pores" refers to a space where hairs are located in the skin of the whole body and is also connected to sebaceous glands. Sebaceous glands attached to the pores secrete sebum. The pores are widened to discharge excessively secreted sebum during puberty when the sebum is excessively secreted, and when sebum stays in the pores in the process of ejecting the pores, stagnant sebum widens the pores. Another cause of widening of the pores is aging of the skin. Skin aging affects the degeneration or reduction of collagen and elastic fibers, which serve to support the pores. The pores naturally widen.

As used herein, "sebum" refers to lipids secreted from sebaceous glands attached to pores. Sebum appears to have a physiological function that is involved in skin drying prevention and lubrication, but excessive secretion of sebum can lead to skin problems such as acne. In addition, the free fatty acids produced by the decomposition of triglycerides by bacteria present in the sebaceous glands play an important role in the development of acne.

A composition for alleviating or improving skin troubles, which is an aspect of the present invention, includes a green tea extract treated with 'foam' and a ginseng extract treated with 'foam', to relieve skin problems remarkably superior to a mixture of green tea extract and ginseng extract. Can be improved or improved.

In a composition for alleviating or improving skin trouble, which is an aspect of the present invention, the composition may have anti-inflammatory activity. The composition, which is an aspect of the present invention, can alleviate skin irritation and relieve inflammation, and thus can be used for anti-inflammatory. Specifically, the composition of the present invention may have an antiallergic effect by inhibiting or inhibiting the expression of prostaglandin or interleukin or inhibiting or inhibiting the activity of prostaglandin or interleukin. Therefore, in the composition of one aspect of the present invention, the composition may be for anti-inflammatory.

 In the composition for alleviating or improving skin trouble, which is an aspect of the present invention, the composition may have anti-allergic ability. The composition, which is an aspect of the present invention, may treat or alleviate allergy by alleviating allergy or inhibiting a mechanism in the body that causes allergy. Specifically, the composition of the present invention may have an antiallergic effect by inhibiting or inhibiting the expression of prostaglandin or interleukin or inhibiting or inhibiting the activity of prostaglandin or interleukin. Therefore, in the composition of one aspect of the present invention, the composition may be for anti-allergic.

In a composition for alleviating or improving skin trouble, which is an aspect of the present invention, the composition may treat or improve atopy. The composition of an aspect of the present invention not only has an anti-inflammatory and anti-allergic effect, but also can enhance skin barrier function and induce differentiation of keratinocytes of the skin, resulting in abnormality of the skin's protective film and causing dry skin. It can be used for the treatment of visible atopy or for the relief of atopic symptoms. Therefore, in the composition of one aspect of the present invention, the composition may be for treating or improving atopy.

In a composition for reducing pores, controlling sebum, or alleviating or improving skin trouble, which is an aspect of the present invention, the composition may have antibacterial activity. The composition of one aspect of the present invention, specifically, shows an excellent antimicrobial activity against acne bacteria. Therefore, the composition can be effectively used in the treatment and alleviation of acne.

 In a composition of one aspect of the invention, the composition may be for antibacterial.

In the composition for reducing pores, sebum control or skin trouble relief or improvement which is an aspect of the present invention, the bacteria may be acne bacteria.

The composition of the present invention may mean alleviation of skin troubles or skin diseases occurring for various reasons, improvement of the condition, or removal or inhibition of various reasons causing skin troubles or skin diseases, and an external skin condition or skin causing them Internal condition can be improved, which is useful for improving skin condition.

In the composition for alleviating or improving skin trouble, which is an aspect of the present invention, the composition may inhibit or inhibit the production of prostaglandins. The composition, which is an aspect of the present invention, may inhibit or inhibit the production of prostaglandin itself or may inhibit or inhibit the activity of prostaglandin. The composition, which is an aspect of the present invention, may also inhibit or inhibit the activity of an up-stream enzyme or protein that produces prostaglandins.

In a composition for alleviating or improving skin trouble, which is an aspect of the present invention, the composition may inhibit or inhibit the production of interleukin. The composition, which is an aspect of the present invention, may inhibit or inhibit the production of interleukin itself or may inhibit or inhibit the activity of interleukin. The composition, which is an aspect of the present invention, may also inhibit or inhibit the activity of an up-stream enzyme or protein that produces interleukin. Specifically, the interleukin may include interleukin-6 or interleukin-8, but is not limited thereto. The composition of the present invention can also be used as a composition for shrinking pores, regulating sebum and improving skin troubles, which suppresses excessive secretion of sebum when applied to the skin, shrinks pores by promoting free radical removal and collagen synthesis, Reducing the expression of inflammatory factors is excellent in inhibiting skin problems.

In the composition for pore reduction or sebum control, which is an aspect of the present invention, the composition includes a green tea extract treated with 'foam' and a ginseng extract treated with 'foam,' and a pore remarkably superior to a mixture of general green tea extract and ginseng extract Reduce sebum or sebum secreted into the skin through pores.

In the composition for pore reduction or sebum control, which is an aspect of the present invention, the composition may provide an effect of shrinking pores widened due to various causes. In addition, in a composition for reducing pores or sebum control, which is an aspect of the present invention, the composition may provide an effect of controlling the amount of sebum secreted and discharged from sebaceous glands.

More specifically, the composition may reduce the inlet of pores or shrink the entire pore, and may inhibit sebum secretion in sebaceous glands and inhibit sebum secreted into pores. In addition, when the generated sebum is suppressed, stagnant sebum that stays in the pores may be reduced, thereby providing an effect of shrinking the pores. Due to the effects as described above, the composition for pore reduction or sebum control of the present invention may alleviate or improve skin troubles such as acne due to sebum secretion.

In the composition for reducing pores, sebum control or skin trouble relief or improvement of one aspect of the present invention, the ginseng extract ginseng extract may comprise a ginseng polysaccharide.

In the composition for reducing pores, sebum control or skin trouble relief or improvement of one aspect of the present invention, the ginseng polysaccharide is a ginseng base polysaccharide, a ginseng chocotan polysaccharide, a ginseng herb polysaccharide, It may be one or more selected from the group consisting of ginseng main polysaccharide, ginseng strong polysaccharide, and ginseng wheat polysaccharide.

In the present specification, "ginseng polysaccharide" is a potent ingredient derived from Panax ginseng CA Meyer, a pectin-like substance having a molecular weight of 34,600, accounting for about 60% of galacturonic acid, and other arabinose. , Rhamnose, glucose, galactose and the like constitute a side chain.

In a composition for reducing pores, controlling sebum, or alleviating or improving skin trouble, which is an aspect of the present invention, the foam-treated green tea extract may include green tea polysaccharide.

In the present specification, "Green tea polysaccharide" is derived from green tea, and unlike polysaccharides found in other plants, it is an acidic polysaccharide group, which is produced by combining sugar components and amino acids made through photosynthesis.

In a composition for pore reduction, sebum control or skin trouble relief or improvement, which is an aspect of the present invention, the green tea polysaccharide is a green tea base polysaccharide, green tea polycarbonate, green tea polysaccharide, It may be one or more selected from the group consisting of green tea syrup polysaccharide, green tea strong polysaccharide and green tea wheat polysaccharide.

The polysaccharide of the present invention may be prepared by a method known in the art, and the method is not particularly limited. Specifically, after extracting the ginseng or green tea leaves at 38 ~ 42 ℃ concentrated and removed impurities with a filter, ethanol is added to the dropwise (dropwise) method and hot air dried to obtain a ginseng or green tea polysaccharide.

In the present specification, the ginseng or green tea base polysaccharide is mixed with ginseng or green tea leaves with brine, and the mixture is roasted at 100 to 180 ° C. for 10 minutes to 1 hour, and then extracted. After concentrating and removing impurities with a filter, ethanol can be obtained by dropwise adding and drying.

In the present specification, the ginseng or green tea chocotan polysaccharide is carbonized outside the ginseng or green tea leaves at 230 to 300 ℃, cooled and extracted by concentrating and removing impurities with a filter, ethanol is added by dropwise method and dried You can get it through

Ginseng or green tea choja polysaccharide in the present specification, after absorbing vinegar in ginseng or green tea leaves and roasted for 10 minutes to 1 hour at 100 to 160 ℃ dried and extracted and concentrated to remove the inflorescences with a filter, ethanol drops It can be obtained by adding in a wise manner and drying.

In the present specification, the ginseng or green tea polysaccharide, soaked in ginseng or green tea leaves, steamed for 30 minutes to 2 hours, dried and extracted, concentrated and removed impurities with a filter, and then ethanol was added in a dropwise manner. Obtained by drying.

In the present specification, ginseng or green tea strong polysaccharide, sprinkle ginger juice on ginseng or green tea leaves, roasted for 10 minutes to 1 hour at 100 to 180 ° C., and then dried and extracted, concentrated and removed sterile water with a filter, and then dropwise ethanol. By adding in a manner and drying.

In the present specification, ginseng or green tea wheat polysaccharide, absorbed honey in ginseng or green tea leaves, roasted for 10 minutes to 1 hour at 100 to 160 ℃ and dried and extracted by extracting and removing fluoride with a filter, ethanol drops It can be obtained by adding in a wise manner and drying.

In the composition for reducing pores, sebum control or skin trouble relief or improvement of the aspect of the present invention, the weight ratio between the extract of the ginseng treated ginseng and the extract of the treated green tea may be 0.1 to 10: 1. It is possible to maximize the synergistic effect of the two components in the weight ratio. From the above point of view, the weight ratio between the extract of the foamed ginseng and the extract of the foamed green tea may be 0.5 to 9.5: 1, 1 to 9: 1 or 1.5 to 8.5: 1.

In one aspect of the present invention, a composition for reducing pores, sebum control, or skin trouble relief or improvement, wherein the composition comprises an extract of ginseng treated ginseng and an extract of ginseng treated green tea in an amount of 0.001 to 10% by weight, based on the total weight of the composition, respectively. can do. If the content of the extract is less than 0.001% by weight, the effect of improving skin troubles such as pore reduction, sebum control or anti-inflammatory, anti-allergic, acne and atopic dermatitis is insignificant. Because it does not. In view of the above, the composition of the present invention is 0.005 to 9.5% by weight, 0.01 to 9% by weight, 0.03 to 8.5% by weight of the extract of the foamed ginseng and the extract of the foamed green tea, respectively, based on the total weight of the composition %, 0.05 to 8% by weight, 0.07 to 7.5% by weight, 0.09 to 7% by weight, 0.1 to 6.5% by weight, 0.3 to 6% by weight, 0.5 to 5.5% by weight or 0.7 to 5% by weight may be included.

In the composition which is one aspect of this invention, the said composition contains a cosmetic composition.

Cosmetic compositions according to the invention may be provided in all formulations suitable for topical application. For example, it may be provided in the form of a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an aqueous phase in an oil phase, a suspension, a solid, a gel, a powder, a paste, a foam, or an aerosol composition. Compositions of such formulations may be prepared according to conventional methods in the art.

The cosmetic composition according to the present invention may include other ingredients in addition to the above-mentioned substances within the range not impairing the main effect, preferably giving a synergistic effect to the main effect. In addition, the cosmetic composition according to the present invention may further include a moisturizer, an emulsifier, a UV absorber, a preservative, a fungicide, an antioxidant, a pH adjuster, organic and inorganic pigments, flavors, coolants or limiting agents. The blending amount of the above components can be easily selected by those skilled in the art within the range that does not impair the object and effect of the present invention, the blending amount may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight based on the total weight of the composition. have.

In a composition that is one aspect of the invention, the composition comprises a pharmaceutical composition.

When the composition according to the present invention is applied to medicines, the composition may be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding a commercially available inorganic or organic carrier.

Examples of preparations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups, pellets and the like. In addition, preparations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method, and surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffers, suspensions, and other commonly used auxiliaries can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenteral, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal, subcutaneous.

The active ingredient of the pharmaceutical composition of the present invention will depend on the age, sex, weight, pathology and severity of the subject to be administered, the route of administration or the judgment of the prescriber. Dosage determination based on these factors is within the level of one skilled in the art and its daily dosage may be, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / kg. May be, but is not limited to.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Comparative Example 1 Preparation of Ginseng Polysaccharide

1 kg of dried white ginseng was pulverized with a blender, 10 liters of purified water was added thereto, stirred and extracted with 40 ° C. hot water for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate are separated by filter cloth filtration and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 the amount of purified water. To this, a concentrated amount of 4 times ethanol was added in a dropwise manner and hot air dried to obtain 65 g of ginseng polysaccharide.

Comparative Example 2 Preparation of Green Tea Polysaccharides

1 kg of the first processed (greening) green tea leaves were pulverized with a blender, 10 liters of purified water was added thereto, stirred and extracted with 40 ° C. hot water for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate are separated by filter cloth filtration and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 the amount of purified water. To this, a concentrated amount of 4 times ethanol was added in a dropwise manner and hot-air dried to give 72 g of green tea polysaccharide.

[Comparative Example 3] Preparation of ginseng extract extract containing ginseng polysaccharide (red ginseng polysaccharide)

The dried ginseng was put in a high pressure steamer and steamed at 97 ° C. for about 4 hours. 1 kg of ginseng was pulverized with a blender, 10 liters of purified water was added thereto, stirred and extracted with 40 ° C. hot water for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate are separated by filter cloth filtration and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 the amount of purified water. Four times the concentrated amount of ethanol was added to the dropwise method and hot-air-dried to obtain 70 g of ginseng polysaccharides (red ginseng polysaccharides) processed using Poje, a herbal processing technique.

Comparative Example 4 Preparation of Green Tea Extract Extract Containing Green Tea Polysaccharide

The first processed (greening) green tea leaves were placed in a high-pressure steamer and steamed at high temperature for about 4 hours at a temperature of 97 ° C., and the steamed green tea leaves having completed the process steps were dried at a temperature of 65 ° C. in a hot air dryer. Thereafter, 1 kg of the processed green tea leaves were pulverized with a blender, and then 10 L of purified water was added thereto, stirred and extracted with 40 ° C. hot water for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate are separated by filter cloth filtration and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 the amount of purified water. Four times the concentrated amount of ethanol was added to the dropwise method and hot-air-dried to obtain 70 g of the processed green tea polysaccharides using a foaming agent, an herbal processing technique.

Comparative Example 5 Preparation of a Mixture of Ginseng Extract and Green Tea Extract

The ginseng polysaccharide and green tea polysaccharide prepared in Comparative Example 1 and Comparative Example 2 were simply mixed in a ratio of 1: 1.

Example 1 Preparation of Mixed Basic Polysaccharides

50% by weight of dry ginseng and 50% by weight of the first-processed green tea are mixed well with brine (2 ~ 3%), sealed and left to be absorbed completely. It was placed in a herb container at 0 ° C. and then roasted for 40 minutes and dried in the shade. 1kg of the processed ginseng and green tea mixture was pulverized with a blender, 10 liters of purified water was added, and the mixture was extracted under reflux three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate are separated by filter cloth filtration and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 of the purified amount. To this, a concentrated amount of ethanol in a multiple of 4 times was added in a dropwise manner and hot-air dried to obtain 70 g of a mixture of ginseng polysaccharide and green tea polysaccharide (mixed base polysaccharide).

Example 2 Preparation of Mixed Pulverized Polysaccharide

50% by weight of dry ginseng and 50% by weight of first-processed (green) carbonized outside at 270 ° C to stop heating and stop heating. After cooling at room temperature, 1kg of processed ginseng and green tea mixture was pulverized with a blender. 10 L was added, and the mixture was extracted under reflux three times, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were concentrated under reduced pressure to 1/10 of the purified water through filtration and centrifugation. To this, a concentrated amount of ethanol in a multiple of 4 times was added in a dropwise manner and hot-air-dried to obtain 72 g of a ginseng polysaccharide and green tea polysaccharide mixture (mixed peat polysaccharide).

Example 3 Preparation of Mixed Supercrystalline Polysaccharide

250 g of vinegar was sufficiently absorbed in a mixture of 50% by weight of dry ginseng and 50% by weight of the first-processed green tea, and then roasted at 130 ° C. for 40 minutes and dried in a shade. 1 kg of this was pulverized with a blender, and 10 liters of purified water was added, followed by extraction under reflux three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were concentrated under reduced pressure to 1/10 of the purified water through filtration and centrifugation. To this, a concentrated amount of ethanol in a multiple of 4 times was added in a dropwise manner, followed by hot air drying to obtain 71 g of a mixture of ginseng polysaccharide and green tea polysaccharide (mixed polysaccharide).

Example 4 Preparation of Mixed Main Polysaccharide

A mixture of 50% by weight of dry ginseng and 50% by weight of the first processed (wet) green tea was soaked in a yellow wine soaked and soaked in a steamer, steamed for 1 hour and 20 minutes, and dried in the shade. 1 kg of this was pulverized with a blender, and 10 liters of purified water was added, followed by extraction under reflux three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were concentrated under reduced pressure to 1/10 of the purified water through filtration and centrifugation. To this, a concentrated amount of 4 times ethanol was added in a dropwise manner and hot-air-dried to obtain 77 g of ginseng polysaccharide and green tea polysaccharide mixture (mixed main polysaccharide).

Example 5 Preparation of Mixed Ferropolysaccharide

After crushing the fresh ginger, add twice as much water to squeeze and squeeze out the juice. Repeat this two to three times to prepare ginger juice, evenly sprayed with a certain amount of ginger juice (10-15% weight compared to ginseng) in a mixture of 50% by weight of dry ginseng and 50% by weight of the first (green) process. Ginger juice is soaked, put in a container at 140 ℃ roasted for 40 minutes and dried in the shade. 1 kg of the processed ginseng and green tea mixture was pulverized with a blender, and 10 liters of purified water was added thereto, extracted three times under reflux, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were concentrated under reduced pressure to 1/10 of the purified water through filtration and centrifugation. To this, a concentrated amount of ethanol in a multiple of 4 times was added in a dropwise manner and hot-air dried to obtain 70 g of a mixture of ginseng polysaccharide and green tea polysaccharide (mixed ferropolysaccharide).

Example 6 Preparation of Mixed Wheat Flour Polysaccharide

In a mixture of 50% by weight of dry ginseng and 50% by weight of the first-processed green tea, absorb a certain amount of honey (20-30% by weight of ginseng) into ginseng, and then stir-fry for 40 minutes at 130 ℃ Dried 1 kg of this was pulverized with a blender, and 10 liters of purified water was added, followed by extraction under reflux three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were concentrated under reduced pressure to 1/10 of the purified water through filtration and centrifugation. To this, a concentrated amount of ethanol in a multiple of 4 times was added in a dropwise manner and hot-air dried to obtain 75 g of a mixture of ginseng polysaccharide and green tea polysaccharide (mixed wheat polysaccharide).

[Test Example 1] Inflammation improving effect

1. Inhibitory Effect of Prostaglandin Production

The anti-inflammatory effect was evaluated as the inhibitory effect of the production of prostaglandins. Effects were measured on macrophages using Comparative Examples 1 to 5 and Examples 1 to 6 above. First, aspirin was added to macrophages taken from the abdominal cavity of mice to a final concentration of 500 uM to irreversibly inhibit the cyclooxygenase (COX) activity remaining in the cells. Then, the suspension was added to each well of a 96-well cell culture tube and incubated for 2 hours in an incubator at 37 ° C. with 5% CO ₂ to attach macrophages to the container surface. Subsequently, the attached macrophages were washed three times with PBS and then used for the inflammation improvement effect test. The cultured ginseng was cultured for 12 hours by adding RPMI medium containing 1% (w / v) LPS to the cultured macrophages 5x10 ⁴ cells / ml to induce the production of prostaglandins and to use processed herbal medicines, forge. Prostaglandins liberated by processing 100 μl of a mixture of polysaccharides and green tea polysaccharides were quantified using an enzyme immunoassay (ELISA).

At this time, the production inhibitory activity of the prostaglandins of the mixture of ginseng polysaccharides and green tea polysaccharides processed by using the herbal formulation technology of Example 1 was 100% of the difference between the prostaglandins produced in the LPS-treated and untreated groups, respectively. The LPS and the sample were treated together and compared with the control group through the percentage of reduced prostaglandin, and the results were determined. The results (prostaglandin production inhibitory effect) are shown in Table 1 below.

Test substance Prostaglandin inhibitory activity (%) NT (Blank) 0% Control group (aspirin treated group) 58.6% Comparative Example 1 20.2% Comparative Example 2 45.5% Comparative Example 3 22.3% Comparative Example 4 46.9% Comparative Example 5 33.8% Example 1 57.2% Example 2 53.3 Example 3 53.9% Example 4 54.4% Example 5 56.7% Example 6 55.2%

As shown in Table 1, in Examples 1 to 6 of the present invention, it can be seen that the effect of inhibiting the production of prostaglandin is very high as in the control group treated with aspirin. In particular, it can be confirmed that the inhibitory effect of prostaglandin production is significantly higher than that of the comparative example without treatment with foam.

Therefore, it can be seen that the composition of the present invention can provide an excellent inflammation improving effect. In addition, it can be seen that the composition of the present invention can prevent and improve skin trouble by inhibiting the expression of prostaglandin, a skin inflammatory factor.

2. Inhibitory Effect of IL-8 Production

One day prior to the experiment, normal human skin keratinocytes (NHEK, obtained from Lonza) were dispensed into 96 well plates at 5x10 ⁴ cells / well, followed by 37 ° C., 5% CO ₂ incubator. incubator for 24 hours. After 24 hours, the cells were washed twice with PBS and changed to serum-free keratinocyte basement media (KBM). Each well was treated with a mixture of ginseng polysaccharides and green tea polysaccharides processed by using the herbal preparation technology of Comparative Examples 1 to 5 and Examples 1 to 6 for each concentration of Table 2, followed by reaction for 30 minutes, followed by PGSA ( 10 μg / ml) was treated to each well. Here, PGSA (peptidoglycan from S. aureus) is a peptidoglycan (peptidoglycan) extracted from Staphylococcus aureus, a major component of the cell wall of Gram-positive bacteria, and the cell membrane components of bacteria are known to cause inflammation, In particular, in staphylococci, about 90% of patients with atopic dermatitis have been reported to cause secondary infection. After incubating for 24 hours at 37 ° C., 5% CO ₂ incubator, the culture medium was taken and subjected to ELISA for Interleukin-8 (IL-8), and the results are shown in Table 2 below. ELISA used the experimental method of the manufacturer (BD science).

Test substance IL-8 secretion (pg / ml) Untreated Control (Control) 755.44 PGSA (10 μg / ml) 4215.46 Comparative Example 1 (50 ppm) + PGSA (10 µg / ml) 3905.21 Comparative Example 2 (50 ppm) + PGSA (10 µg / ml) 2975.94 Comparative Example 3 (25 ppm) + PGSA (10 μg / ml) 3552.40 Comparative Example 3 (50 ppm) + PGSA (10 μg / ml) 2943.38 Comparative Example 4 (25 ppm) + PGSA (10 μg / ml) 3319.48 Comparative Example 4 (50 ppm) + PGSA (10 µg / ml) 2879.92 Comparative Example 5 (50 ppm) + PGSA (10 µg / ml) 3492.54 Example 1 (25 ppm) + PGSA (10 μg / ml) 2531.32 Example 1 (50 ppm) + PGSA (10 μg / ml) 1856.93 Example 2 (25 ppm) + PGSA (10 μg / ml) 2775.88 Example 2 (50 ppm) + PGSA (10 μg / ml) 2224.36 Example 3 (25 ppm) + PGSA (10 μg / ml) 2798.84 Example 3 (50 ppm) + PGSA (10 μg / ml) 2210.43 Example 4 (25 ppm) + PGSA (10 μg / ml) 2632.02 Example 4 (50 ppm) + PGSA (10 μg / ml) 1954.79 Example 5 (25 ppm) + PGSA (10 μg / ml) 2613.31 Example 5 (50 ppm) + PGSA (10 μg / ml) 2003.95 Example 6 (25 ppm) + PGSA (10 μg / ml) 2690.54 Example 6 (50 ppm) + PGSA (10 μg / ml) 2100.46

As can be seen in Table 2, the composition of the present invention was confirmed to significantly reduce or inhibit the secretion of IL-8 increased by PGSA and LPS. In particular, it was confirmed to have a significantly higher inhibitory effect on IL-8 secretion than the comparative example without treatment with foam.

As such, it can be seen that the composition of the present invention will significantly reduce the secretion of IL-8 increased by PGSA and thus provide an excellent anti-inflammatory effect.

[ Test Example  2] itching relief assessment

One day prior to the experiment, the keratinocytes (Cell name: HaCaT, obtained from ATCC) were dispensed into 96 well plates at ⁴ × 10 ⁴ cells / well, and then in a 37 ° C., 5% CO ₂ incubator. Incubated for 24 hours. After 24 hours, wash 96 well plates twice with Hanks'Balanced Salt solution (HBSS) buffer, and then add reaction buffer (2 μM Fluo-4-AM, 20% pluronic acid, 2.5 mM probenecid) to the cells. gave. After reaction at 37 ° C., 5% CO ₂ incubator for 30 minutes, and at room temperature for 30 minutes, the cells were washed twice with HBSS buffer and treated with cells of Examples 1 to 6 as shown in Table 3 below.

After reaction for 10 minutes to process the 2U / ml trypsin (Trypsin) or 5μM PAR-2 activation peptide (SLIGKV) and was measured in Ca ^{2 +} concentration in the cell 80 seconds. In Ca ^{2 +} concentration in the measurement cell presentation flex 3: was used (FlexStation 3 Molecular Device, USA) . Ginseng polysaccharides and green tea polysaccharide mixtures processed by using the herbal preparation techniques of Comparative Examples 1 to 5 and Examples 1 to 6 were treated with 2U / ml trypsin or 5 μM PAR-2 active peptide (SLIGKV) for 80 seconds. Find the difference between the minimum and maximum values obtained by measuring the flex, then compare the value with the difference between the minimum and maximum values for 2U / ml trypsin or 5 μM PAR-2 active peptide (SLIGKV) treatment. The inhibition rate (%) for the intracellular influx of calcium ions is shown in Table 3 below.

Test substance concentration % Inhibition of intracellular influx of calcium ions Trypsin (2U / ml) PAR-2 active peptide (5 μM) Comparative Example 1 0.1% 14 17 0.5% 16 19 Comparative Example 2 0.1% 19 22 0.5% 22 27 Comparative Example 3 0.1% 14 17 0.5% 17 20 Comparative Example 4 0.1% 20 23 0.5% 24 28 Comparative Example 5 0.1% 16 19 0.5% 19 23 Example 1 0.05% 24 27 0.1% 28 30 0.5% 36 39 Example 2 0.05% 24 26 0.1% 28 31 0.5% 36 40 Example 3 0.05% 25 27 0.1% 28 31 0.5% 36 41 Example 4 0.05% 27 29 0.1% 29 32 0.5% 37 42 Example 5 0.05% 26 28 0.1% 28 32 0.5% 37 41 Example 6 0.05% 25 27 0.1% 28 31 0.5% 36 40

As can be seen in Table 3, the composition of the present invention is to reduce the influx of intracellular calcium ions by trypsin or PAR-2 active peptide (SLIGKV), and to increase the treatment concentration significantly increased the influx of intracellular calcium ions It was confirmed that the decrease. In particular, the composition of the present invention can confirm the effect of inhibiting significantly higher calcium ion influx than the comparative example without treatment.

Therefore, the composition of the present invention can provide excellent anti-pruritic effect by effectively inhibiting PAR-2 activity causing itching.

Test Example 5 Antibacterial Activity Test against Acne Bacteria

Formulation Examples 1 to 9 and Comparative Formulation Examples 1 to 7 were prepared according to the ingredients and the contents (% by weight) shown in Tables 4 and 5 below. Specifically, Formulation Examples 1 to 9 are formulated with a mixture of extract of ginseng treated ginseng and green tea treated with foam, Comparative Formulation Example 1 does not contain any active ingredients for acne skin improvement, Comparative Formulation Example 2 is antibacterial activity It contains erythromycin, which is widely used as an acne treatment, as a reference standard.

Formulation Examples 1 to 6 and Comparative Formulation Examples 1 to 7 were prepared as follows. The components of phase A of Tables 4, 5 and 6 below were completely dissolved and the components of phase B were completely dissolved in a separate dissolution bath, followed by mixing solubilization by adding phase B to phase A. The ingredients of phase C were added thereto according to the blending ratios described in Tables 4, 5 and 6 to homogenize the mixture and then filtered to prepare the compositions.

The unit of content ratio of the following compounding component is weight%.

Compounding Ingredients (wt%) Comparative Formulation Example 1 Comparative Formulation Example 2 A Purified water To 100 To 100 EDTA-2Na 0.02 0.02 glycerin 5.0 5.0 B ethanol 2.0 2.0 PEG-60 Cured Castor Oil
(hydrogenated castor oil) 0.4 0.4 Perfume 0.04 0.04 C Example 1 - - Example 2 - - Example 3 - - Erythromycin - 5.0

Compounding Ingredients (wt%) Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Formulation Example 6 A Purified water To 100 To 100 To 100 To 100 To 100 To 100 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 B ethanol 2.0 2.0 2.0 2.0 2.0 2.0 PEG-60 Cured Castor Oil
(hydrogenated castor oil) 0.4 0.4 0.4 0.4 0.4 0.4 Perfume 0.04 0.04 0.04 0.04 0.04 0.04 C Example 1 5.0 - - - - - Example 2 - 5.0 - - - - Example 3 - - 5.0 - - - Example 4 - - - 5.0 - - Example 5 - - - - 5.0 - Example 6 - - - - - 5.0

Compounding Ingredients (wt%) Comparative Formulation Example 3 Comparative Formulation Example 4 Comparative Formulation Example 5 Comparative Formulation Example 6 Comparative Formulation Example 7 A Purified water To 100 To 100 To 100 To 100 To 100 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 glycerin 5.0 5.0 5.0 5.0 5.0 B ethanol 2.0 2.0 2.0 2.0 2.0 PEG-60 Cured Castor Oil
(hydrogenated castor oil) 0.4 0.4 0.4 0.4 0.4 Perfume 0.04 0.04 0.04 0.04 0.04 C Comparative Example 1 5.0 - - - - Comparative Example 2 - 5.0 - - - Comparative Example 3 - - 5.0 - - Comparative Example 4 - - - 5.0 - Comparative Example 5 - - - - 5.0

The antibacterial activity was tested against propionibacterium acnes (ATCC 6919: medium-BHI broth), which is an acne-causing strain, with each cosmetic composition prepared in the formulations of Formulation Examples 1 to 6 and Comparative Formulation Examples 1 to 7. It was.

The antibacterial test method for acne bacteria is as follows.

(1) Test bacteria preparation

Propionibacterium acnes was used as a culture broth inoculated in BHI broth and anaerobic culture.

(2) dilution solution preparation

0.15 ml of the test bacteria was added to 15 ml of BHI broth (pH 6.8) or LB broth (pH 4.5) and mixed well as a dilution solution.

(3) sample preparation

Each of the compositions prepared from Formulation Examples 1-6 and Comparative Formulation Examples 1-7 was used as a sample.

(4) antimicrobial activity test

1) Insert the sample to the starting concentration in the first well of 96-well cell culture tube (96 well plate) and add 200 μl of the total amount into the dilution solution.

2) Mix the mixed solution in the first row well, take 100μl into the second row, mix well, and then add 100μl to the third row and perform double dilution.

3) After incubation for 24 hours and 48 hours at 32 ℃, determine the growth of bacteria to the extent of suspension and determine the minimum concentration without growth of bacteria as MIC (Minimum Inhibitory Concentration) value. If the mixed solution is opaque and it is difficult to determine the growth of bacteria, check through a microscope.

The antimicrobial activity test results for acne bacteria are shown in Table 7 below. MIC is expressed in terms of the concentration of the active ingredient contained in the formulation.

Item pH Propionibacterium Acnes Formulation Example 1 5.7 > 48 ppm Formulation Example 2 5.7 > 55 ppm Formulation Example 3 5.7 > 54 ppm Formulation Example 4 5.7 > 53 ppm Formulation Example 5 5.7 > 50 ppm Formulation Example 6 5.7 > 52 ppm Comparative Formulation Example 1 5.7 Highest concentration (no antibacterial activity) Comparative Formulation Example 2 5.7 > 82 ppm Comparative Formulation Example 3 5.7 > 155 ppm Comparative Formulation Example 4 5.7 > 100 ppm Comparative Formulation Example 5 5.7 > 147 ppm Comparative Formulation Example 6 5.7 > 100 ppm Comparative Formulation Example 7 5.7 > 129 ppm

The smaller the ppm concentration in MIC, the more effective the antimicrobial activity against acne. In the case of Formulation Examples 1 to 6 of the present invention, it has a significantly lower ppm concentration than Comparative Formulation Example 2 using erythromycin, which is a known acne treatment agent, and thus it was confirmed that it has very good antibacterial activity. In addition, it was confirmed that Formulation Examples 1 to 6 of the present invention have a significantly higher antimicrobial effect than Comparative Formulation Examples 3 to 7.

[ Test Example  6] Test for improving atopic symptoms in human body

A cream comprising a foamed ginseng extract and a foamed green tea extract was prepared with the compositions shown in Tables 8 and 9.

Compounding Ingredients (wt%) Formulation Example 7 Formulation Example 8 Formulation Example 9 Formulation Example 10 Formulation Example 11 Formulation Example 12 Purified water To 100 To 100 To 100 To 100 To 100 To 100 Example 1 1.0 - - - - - Example 2 - 1.0 - - - - Example 3 - - 1.0 - - - Example 4 - - - 1.0 - - Example 5 - - - - 1.0 - Example 6 - - - - - 1.0 Vegetable Cured Oil 1.50 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 2.00 Glycerol Stearate 1.00 1.00 1.00 1.00 1.00 1.00 Polyglyceryl-10 Pentastearate & Behenyl Alcohol & Sodium Stearoyl Lactylate 1.00 1.00 1.00 1.00 1.00 1.00 Arachidil Behenyl Alcohol & Arachidil Glucoside 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol & Cetearyl Glucoside 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 Stearate & Glycerol Olate & Propylene Glycol 1.50 1.50 1.50 1.50 1.50 1.50 Caprylic / Capric Triglycerides 11.00 11.00 11.00 11.00 11.00 11.00 Cyclomedicon 6.00 6.00 6.00 6.00 6.00 6.00 Triethanolamine 0.1 0.1 0.1 0.1 0.1 0.1

Compounding Ingredients (wt%) Comparative Formulation Example 8 Comparative Formulation Example 9 Comparative Formulation Example 10 Comparative Formulation Example 11 Comparative Formulation Example 12 Comparative Formulation Example 13 Purified water To 100 To 100 To 100 To 100 To 100 To 100 Comparative Example 1 - 1.0 - - - - Comparative Example 2 - - 1.0 - - - Comparative Example 3 - - - 1.0 - - Comparative Example 4 - - - - 1.0 - Comparative Example 5 - - - - - 1.0 Vegetable Cured Oil 1.50 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 2.00 Glycerol Stearate 1.00 1.00 1.00 1.00 1.00 1.00 Polyglyceryl-10 Pentastearate &
Behenyl Alcohol & Sodium Stearoyl Lactylate 1.00 1.00 1.00 1.00 1.00 1.00 Arachidil Behenyl Alcohol & Arachidil Glucoside 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol & Cetearyl Glucoside 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 Stearate & Glycerol Olate & Propylene Glycol 1.50 1.50 1.50 1.50 1.50 1.50 Caprylic / Capric Triglycerides 11.00 11.00 11.00 11.00 11.00 11.00 Cyclomedicon 6.00 6.00 6.00 6.00 6.00 6.00 Triethanolamine 0.1 0.1 0.1 0.1 0.1 0.1

The creams of Formulation Examples 7-12 and Comparative Formulation Examples 8-13 prepared with the compositions of Table 8 and Table 9 were applied to the panels to evaluate the degree of atopic dermatitis improvement. For 120 panelists who think there are symptoms of dermatitis showing similar symptoms, each group of 10 persons is divided into 12 groups, each of the composition of Table 6 twice daily for 8 weeks at a temperature of 24 to 26 ° C and a humidity of 75%. It was applied to the face. At the end of the study, subjects were asked to complete a questionnaire and subjective efficacy evaluations were conducted. The questionnaire included symptoms of atopic dermatitis such as "pruritus", "dry", "keratogenesis", "dandruff", "erythema", "swelling", "skin cracking", "duty and eczema", and "tertiary disease". After dividing the above symptoms, the degree of improvement for each symptom was evaluated and averaged to evaluate the atopic symptoms. The results are shown in Table 10.

Application group (number of the respondents-person) Markedly improved Improving No change worse Formulation Example 7 4 5 One 0 Formulation Example 8 3 5 2 0 Formulation Example 9 2 7 One 0 Formulation Example 10 3 6 One 0 Formulation Example 11 4 5 One 0 Formulation Example 12 3 6 One 0 Comparative Formulation Example 8 0 5 5 0 Comparative Formulation Example 9 0 6 4 0 Comparative Formulation Example 10 One 7 3 0 Comparative Formulation Example 11 One 6 3 0 Comparative Formulation Example 12 One 7 3 0 Comparative Formulation Example 13 One 6 3 0

As a result, it was confirmed that the composition of the present invention has a better effect in improving the symptoms of atopic dermatitis than Comparative Formulation Example 8. In addition, it was confirmed that the composition of the present invention has an effect of improving atopic dermatitis than Comparative Formulation Examples 9 to 13 containing Comparative Examples 1 to 5.

[ Test Example  7] sebum secretion inhibitory effect

In order to determine the effect of inhibiting sebum secretion of the Examples and Comparative Examples was evaluated as follows. The creams of Formulation Examples 7-12 and Comparative Formulation Examples 8-13, which were prepared with the compositions of Tables 8 and 9 above, were selected and divided into 12 sets of 8 groups of 8 subjects, each of whom were selected to feel sebum secretion. 8-9] were correct every day for four weeks. Determination of the effect of sebum reduction was measured using a sebum measuring device, the results are shown in Table 11 below.

Subject Sebum reduction rate after 2 weeks Sebum reduction rate after 4 weeks Formulation Example 7 15.9 ± 2.9% 18.1 ± 2.5% Formulation Example 8 15.4 ± 2.5% 17.3 ± 2.6% Formulation Example 9 15.3 ± 2.8% 17.4 ± 2.6% Formulation Example 10 15.5 ± 2.3% 17.5 ± 2.7% Formulation Example 11 15.7 ± 2.5% 17.7 ± 2.9% Formulation Example 12 15.5 ± 2.6% 17.4 ± 2.8% Comparative Formulation Example 8 5.1 ± 1.9% 6.5 ± 1.8% Comparative Formulation Example 9 5.2 ± 1.8% 6.5% ± 2.0 Comparative Formulation Example 10 5.4 ± 1.8% 6.6 ± 2.1% Comparative Formulation Example 11 5.3 ± 2.0% 6.4 ± 1.8% Comparative Formulation Example 12 5.2 ± 1.8% 6.3 ± 1.7% Comparative Formulation Example 13 5.2 ± 1.5% 6.5 ± 2.0%

From the results in Table 11, Formulation Examples 7 to 12 containing the green tea ginseng polysaccharide treated with various methods according to the present invention as an active ingredient are more effectively sebum secreted than Comparative Examples 8 to 13 containing no It was found that it can be suppressed.

Therefore, the external preparation composition for skin containing the saponified green tea ginseng extract according to the present invention has an excellent sebum secretion inhibitory effect.

[ Test Example  8] Pore Shrinkage Effect Test

In order to determine the pore reduction effect of the Examples and Comparative Examples was evaluated as follows. 96 males and females with large pore sizes were selected and divided into 12 sets of 8 groups each, and the cream of Formulation Examples 7-12 and Comparative Formulation Examples 8-13 prepared on the face for 4 weeks every day for 4 weeks. Correctly Determination of the effect of pore reduction was made by visual assessment of experts by taking pictures before and after 4 weeks of the experiment. The results are shown in Table 12 below. (Rating rating: 0. not reduced at all-5. very reduced)

matter Rating Formulation Example 7 3.3 Formulation Example 8 2.8 Formulation Example 9 2.9 Formulation Example 10 3.1 Formulation Example 11 3.2 Formulation Example 12 3.0 Comparative Formulation Example 8 0.9 Comparative Formulation Example 9 0.8 Comparative Formulation Example 10 0.8 Comparative Formulation Example 11 1.0 Comparative Formulation Example 12 0.9 Comparative Formulation Example 13 1.0

From the results in Table 12, Formulation Examples 7 to 12 containing green tea ginseng polysaccharide treated with various methods according to the present invention as an active ingredient have an effect of reducing the size of pores than Comparative Examples 8 to 13 that do not contain it. Was found to be excellent.

[ Test Example  9] Sensory evaluation of pore reduction effect

A lotion containing foamed ginseng extract and foamed green tea extract was prepared with the composition specified in Table 13 below.

Compounding ingredient
(weight %) Formulation Example 13 Formulation Example 14 Formulation Example 15 Formulation Example 16 Formulation Example 17 Formulation Example 18 Comparative Formulation Example 14 Comparative Formulation Example 15 Comparative Formulation Example 16 Comparative Formulation Example 17 Comparative Formulation Example 18 Comparative Formulation Example 19 Example 1 0.2 - - - - - - - - - - - Example 2 - 0.2 - - - - - - - - - - Example 3 - - 0.2 - - - - - - - - - Example 4 - - - 0.2 - - - - - - - - Example 5 - - - - 0.2 - - - - - - - Example 6 - - - - - 0.2 - - - - - - Comparative Example 1 - - - - - - - 0.2 - - - - Comparative Example 2 - - - - - - - - 0.2 - - - Comparative Example 3 - - - - - - - - - 0.2 - - Comparative Example 4 - - - - - - - - - - 0.2 - Comparative Example 5 - - - - - - - - - - - 0.2 glycerin 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Butylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Propylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Carboxy Vinyl Polymer 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Fiji-12 nonylphenyl ether 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Polysorbate 80 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 ethanol 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 Triethanolamine 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Preservative, coloring, flavoring Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount

Among 120 women who are 25 to 35 years old who are suffering from large pores, each group consists of 10 groups of 12 people, each of which consists of 12 sets of Formulation Examples 13-18 and Comparative Formulation Examples 14-19, respectively. The composition was allowed to be used, and the degree of skin improvement was determined by self-measurement according to the following evaluation criteria. The results are shown in Table 14 below.

<Evaluation Criteria>

+++: Very good improvement.

++: Significant improvement.

+: A slight improvement.

±: No improvement but no deterioration.

-: No improvement, rather worse.

Subject 1 week 2 weeks 3 weeks 4 Weeks Formulation Example 13 ++ ++ +++ +++ Formulation Example 14 ± + ++ +++ Formulation Example 15 ± + ++ +++ Formulation Example 16 + + ++ +++ Formulation Example 17 + ++ +++ +++ Formulation Example 18 + ++ ++ +++ Comparative Formulation Example 14 ± ± + + Comparative Formulation Example 15 ± ± ± ± Comparative Formulation Example 16 ± ± ± ± Comparative Formulation Example 17 ± ± + + Comparative Formulation Example 18 ± ± ± ± Comparative Formulation Example 19 ± ± + +

As can be seen in Table 14, the formulation examples (13 ~ 18) comprising the foamed green tea ginseng extract according to one or more of the present invention, the pores shrinking effect significantly superior to the comparative formulation (14 ~ 19) that does not include this Indicated.

Therefore, the composition for external application for skin containing the green tea ginseng extract treated with foam according to the present invention has an excellent pore reduction effect. In addition, sebum is secreted from the pores, so as the pores shrink, sebum secretion is also accompanied by an inhibitory effect.

Examples of the formulation of the composition according to the present invention are described below, but are also applicable to various other formulations, which are intended to explain in detail only and not intended to limit the present invention.

Preparation Example 1 Soft Capsule

150 mg of ginseng extract ginseng extract, 150 mg of ginseng treated green tea extract, 2 mg palm oil, 8 mg palm wax oil, 4 mg of lead wax and 6 mg of lecithin, and filled with 400 mg per capsule according to a conventional method to soft capsule Prepared.

Preparation Example 2 Tablet

150 mg of foamed ginseng extract, 150 mg of foamed green tea extract, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate and 40 mg of 30% ethanol were added to form granules. Dried at 占 폚 and compressed into tablets using a tablet machine.

Preparation Example 3 Granule

150 mg of ginseng treated ginseng extract, 150 mg of ginseng treated green tea extract, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch were mixed and 100 mg of 30% ethanol was added to form granules. Formed and filled into fabric. The final weight of the content was 1 g.

Formulation Example 1 Flexible Cosmetic (Skin Lotion)

According to the composition described in Table 15 below to prepare a flexible cosmetic water in a conventional manner.

Compounding ingredient Content (weight%) Extract of ginseng and green tea (Ginseng: Green tea = 1: 1) 0.2 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 Fiji-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation Example 2 Nutritious Longevity (Milk Lotion)

Nutrients were prepared in a conventional manner according to the composition described in Table 16 below.

Compounding ingredient Content (weight%) Extract of ginseng and green tea (Ginseng: Green tea = 1: 1) 1.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Beeswax 4.0 Polysorbate 60 1.5 Caprylic / Capric Triglycerides 5.0 Squalane 5.0 Sorbitassquioleate 1.5 Liquid paraffin 0.5 Cetearyl Alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation Example 3 Nutrition Cream

Nutritional cream was prepared in a conventional manner according to the composition described in Table 17 below.

Compounding ingredient Content (% by weight) Extract of ginseng and green tea (Ginseng: Green tea = 1: 1) 2.0 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Squalane 5.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation Example 4 Massage Cream

Massage creams were prepared in a conventional manner according to the composition described in Table 18 below.

Compounding ingredient Content (% by weight) Extract of ginseng and green tea (Ginseng: Green tea = 1: 1) 3.0 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Beeswax 4.0 Cetearyl Glucoside 1.5 Sesqui oleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, flavoring Quantity Purified water Remaining amount

[Formulation Example 5] Pack

To prepare a pack in a conventional manner according to the composition described in Table 19 below.

Compounding ingredient Content (% by weight) Extract of ginseng and green tea (Ginseng: Green tea = 1: 1) 0.2 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl Phenyl Ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 quantity Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation Example 6 Ointment

The ointment was prepared in a conventional manner according to the composition described in Table 20 below.

Compounding ingredient Content (% by weight) Extract of ginseng and green tea (Ginseng: Green tea = 1: 1) 0.2 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Squalane 1.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Cetearyl Alcohol 1.0 Beeswax 4.0 Preservative, coloring, flavoring Quantity Purified water Remaining amount

As described above in detail the specific parts of the present invention, it is apparent to those skilled in the art that such specific description is merely a preferred embodiment, thereby not limiting the scope of the present invention. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (14)

A mixture of ginseng base polysaccharide and green tea base polysaccharide; A mixture of ginseng and peat polysaccharides and green tea and peat polysaccharides; A mixture of ginseng herb polysaccharide and green tea herb polysaccharide; A mixture of ginseng synovial polysaccharide and green tea syrup polysaccharide; A mixture of ginseng strong polysaccharide and green tea strong polysaccharide; And ginseng wheat polysaccharide and green tea wheat polysaccharide mixture of at least one selected from the group consisting of ginseng extract ginseng extract and ginseng treated green tea extract as an active ingredient composition for alleviating or improving skin problems. The method of claim 1,
Wherein said skin trouble is one or more of inflammation, allergy, atopy, fungal infections and acne. The method of claim 2,
The bacterium is an acne bacterium, a composition for alleviating or improving skin trouble. The composition for alleviating or improving skin trouble according to claim 1, wherein the composition inhibits or inhibits production of prostaglandins. The composition of claim 1, wherein the composition inhibits or inhibits the production of interleukin. The composition of claim 1, wherein the weight ratio between ginseng polysaccharide and green tea polysaccharide is 0.1 to 10: 1. The composition of claim 1, wherein the composition comprises ginseng polysaccharide and green tea polysaccharide in an amount of 0.001 to 10% by weight, respectively, based on the total weight of the composition. A mixture of ginseng base polysaccharide and green tea base polysaccharide; A mixture of ginseng chotan polysaccharide and green tea base polysaccharide; Ginseng plant polysaccharide and green tea plant polysaccharide; Ginseng main polysaccharide and green tea main polysaccharide; Ginseng strong polysaccharide and green tea strong polysaccharide; And ginseng mil polysaccharide and green tea mil polysaccharide, the composition for pore reduction or sebum control comprising a mixture of one or more ginseng-treated ginseng extracts and ginseng-treated green tea extracts selected from the group consisting of active ingredients. The composition of claim 8, wherein the weight ratio between the ginseng polysaccharide and the green tea polysaccharide is 0.1 to 10: 1. The composition of claim 8, wherein the composition comprises ginseng polysaccharide and green tea polysaccharide in an amount of 0.001 to 10% by weight, respectively, based on the total weight of the composition. The composition according to any one of claims 1 to 7, wherein the composition is a cosmetic composition. The composition of claim 1, wherein the composition is a pharmaceutical composition. The composition according to any one of claims 8 to 10, wherein the composition is a cosmetic composition. The composition of claim 8, wherein the composition is a pharmaceutical composition.