KR102014962B1 - Composition for antiinflammation containing extract of soybean pod - Google Patents

Abstract

The present invention relates to an anti-inflammatory, anti-allergic, atopic or therapeutic or moisturizing composition comprising soybean pod extract. The composition of the present invention has an anti-inflammatory effect that can inhibit or inhibit the production of a causative agent of inflammation, thereby treating or alleviating inflammation, and is useful as it can treat or alleviate allergy. In addition, the composition of the present invention can treat or improve atopy, and has a moisturizing effect by promoting differentiation in keratinocytes, and thus is useful for treating or relieving symptoms associated with dry skin. In particular, the composition of the present invention shows a better effect than the soybean extract in anti-inflammatory, anti-allergic, atopic treatment or improvement or moisturizing ability. The compositions of the present invention also contain very little irritation to organisms, including extracts of natural origin obtained from natural products.

Description

Anti-inflammatory composition comprising soybean pod extract {COMPOSITION FOR ANTIINFLAMMATION CONTAINING EXTRACT OF SOYBEAN POD}

The present invention relates to an anti-inflammatory, anti-allergic, atopic or therapeutic or moisturizing composition comprising soybean pod extract.

Human skin is the body's primary protective barrier, which protects the body's organs from changes in temperature and humidity, and from external environmental stimuli such as ultraviolet rays and pollutants. Undergo a change. That is, internally, the secretion of various hormones that regulate metabolism decreases, and the function of immune cells and the activity of cells decreases, thereby reducing the biosynthesis of immune proteins and constituent proteins necessary for living organisms. Due to the increase in the amount of ultraviolet rays that reach the surface of the sun's rays and intensifying environmental pollution, free radicals and free radicals increase, thereby reducing the thickness of the skin, increasing wrinkles, reducing elasticity, The color of the skin becomes dull, skin problems frequently occur, and there are various changes such as blemishes, freckles, and black mushrooms.

In order to prevent changes in the skin condition caused by these internal and external factors and to maintain a healthy skin condition, the skin condition is improved by adding bioactive substances obtained from various known animals, plants, and microorganisms to cosmetics. Efforts have been made.

Accordingly, the present inventors have confirmed that the bean pod extract can provide not only an anti-inflammatory and anti-allergic effect but also an atopic symptom improvement effect, and can provide a skin condition improvement effect such as a skin moisturizing function effect and completed the present invention. .

Republic of Korea Patent Publication No. 1998-077876

An object of the present invention is to provide a composition comprising a natural extract that can be used for anti-inflammatory, anti-allergic, atopic treatment or improvement or moisturizing.

In order to achieve the above object, the present invention provides a composition for anti-inflammatory comprising a bean pod extract as an active ingredient.

The present invention also provides an anti-allergic composition comprising a bean pod extract as an active ingredient, a composition for treating or improving atopy comprising the bean pod extract as an active ingredient, and a moisturizing composition comprising the bean pod extract as an active ingredient. to provide.

The composition of the present invention has an anti-inflammatory effect that can inhibit or inhibit the production of a causative agent of inflammation, thereby treating or alleviating inflammation, and is useful as it can treat or alleviate allergy. In addition, the composition of the present invention can treat or improve atopy, and has a moisturizing effect by promoting differentiation in keratinocytes, which is useful for the treatment or alleviation of symptoms associated with dry skin, and can relieve skin itching. have. In particular, the composition of the present invention shows a better effect than the soybean extract in anti-inflammatory, anti-allergic, atopic treatment or improvement or moisturizing ability. The compositions of the present invention also contain very little irritation to organisms, including extracts of natural origin obtained from natural products.

In the present specification, 'bean' is not limited in kind. In the composition which is one aspect of the present invention, specifically, the soybean of the present specification is selected from the group consisting of frosted, seomoktae, heuktae, chungtae, yellow, hedge bean, kidney bean, zebra kidney bean, red bean, soybean, bean sprout bean and soybean It may include any one or more, but is not limited thereto.

In the present specification, the 'bean pod' means a shell containing the bean, that is, the shell surrounding the bean.

In the present specification, 'bean pod extract' is, for example, in the extraction process, washed and dried bean pods or powdered soybean pod powder in water or an organic solvent, extracted and deposited, and then filtered through filter cloth and centrifugation. The residue and the filtrate are separated, and the filtrate is concentrated under reduced pressure to obtain a bean pod extract. The organic solvent usable in the present invention may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water, and considering the safety of the raw material, preferably water or 30 to 70% concentration. Ethanol is used. After obtaining the extract using a solvent in the above can be obtained by cooling, heating and filtration at room temperature in a conventional manner known in the art to obtain a liquid, or may further evaporate the solvent, spray drying or freeze drying, but The present invention is not limited thereto, and any method generally used in the art may be used without limitation.

The present invention relates to an anti-inflammatory composition comprising a bean pod extract as an active ingredient in one aspect. The composition, which is an aspect of the present invention, can alleviate skin irritation and relieve inflammation, and thus can be used for anti-inflammatory. Specifically, the composition of the present invention may have an antiallergic effect by inhibiting or inhibiting the expression of prostaglandin or interleukin or inhibiting or inhibiting the activity of prostaglandin or interleukin.

The present invention relates to an anti-allergic composition comprising soybean pod extract as an active ingredient in one aspect. The composition, which is an aspect of the present invention, may treat or alleviate allergy by alleviating allergy or inhibiting a mechanism in the body that causes allergy. Specifically, the composition of the present invention may have an antiallergic effect by inhibiting or inhibiting the expression of prostaglandin or interleukin or inhibiting or inhibiting the activity of prostaglandin or interleukin.

The present invention relates to a composition for treating or improving atopy, which includes, in one aspect, a bean pod extract as an active ingredient. The composition of an aspect of the present invention not only has an anti-inflammatory and anti-allergic effect, but also can enhance skin barrier function and induce differentiation of keratinocytes of the skin, resulting in abnormality of the skin's protective film and causing dry skin. It can be used for the treatment of visible atopy or for the relief of atopic symptoms.

The present invention relates to a moisturizing composition comprising a bean pod extract as an active ingredient in one aspect. The composition, which is an aspect of the present invention, may enhance skin barrier function and induce differentiation of keratinocytes of the skin to increase the moisture of the skin, thereby making the skin moist. Therefore, the composition, which is an aspect of the present invention, may be usefully used as a composition for preventing or improving skin dryness, atopic dermatitis, contact dermatitis, or psoriasis caused by incomplete epidermal differentiation.

The composition of the present invention may mean alleviation of skin troubles or skin diseases occurring for various reasons, improvement of the condition, or removal or inhibition of various reasons causing skin troubles or skin diseases, and an external skin condition or skin causing them Internal condition can be improved, which is useful for improving skin condition.

In a composition of one aspect of the present invention, the composition may include 0.001 to 10% by weight of the bean pod extract based on the total weight of the composition.

When the content of the bean pod extract is less than 0.001% by weight based on the total weight of the composition, the skin troubles such as anti-inflammatory, anti-allergic and atopy are improved and the skin moisturizing effect is insignificant. Because it does not appear. In view of the above, the composition of the present invention, 0.005 to 9.5% by weight, 0.01 to 9% by weight, 0.03 to 8.5% by weight, 0.05 to 8% by weight, 0.07 to 7.5% by weight, 0.09 to the total weight of the composition It may include 7% by weight, 0.1 to 6.5% by weight, 0.3 to 6% by weight, 0.5 to 5.5% by weight or 0.7 to 5% by weight of the bean pod extract.

In the composition of one aspect of the invention, the soybean is Seoritae ( Glycin max MERR), Seomoktae (Seomoktae, Rhynchosia Nolubilis ), Black soybean, Glycine max (L.) Merr.), Cheongtae (blue bean, Glycime) max MERR), yellow bean, Glycime max MERR), field bean, Vicia faba ), Kidney bean ( Phaseolus) vulgaris , pinto bean, Phaseolus vulgaris L.), small red bean, Vigna angularis ), Soybeans (small black bean, Phaseolus angularis WF WIGHT.), Sprouting bean, Glycine max (L.) Merr.) and soybean ( Glycine) max ) and any one or more selected from the group consisting of.

In a composition which is one aspect of the present invention, the composition may inhibit or inhibit the production of prostaglandins. The composition, which is an aspect of the present invention, may inhibit or inhibit the production of prostaglandin itself or may inhibit or inhibit the activity of prostaglandin. The composition, which is an aspect of the present invention, may also inhibit or inhibit the activity of an up-stream enzyme or protein that produces prostaglandins.

In a composition which is one aspect of the present invention, the composition may inhibit or inhibit the production of interleukin. The composition, which is an aspect of the present invention, may inhibit or inhibit the production of interleukin itself or may inhibit or inhibit the activity of interleukin. The composition, which is an aspect of the present invention, may also inhibit or inhibit the activity of an up-stream enzyme or protein that produces interleukin. Specifically, the interleukin may include interleukin-6 or interleukin-8, but is not limited thereto.

In the composition which is one aspect of this invention, the said composition contains a cosmetic composition.

When formulating the external composition for skin according to the present invention in the form of cosmetics, soft cosmetics, astringent cosmetics, nourishing cosmetics, eye cream, nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence or pack, etc. It may be formulated in the form of, the formulation is not particularly limited. In addition, the composition according to the present invention can be used for fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or non- With ionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients commonly used in cosmetics. It may contain adjuvants commonly used in the same cosmetic or dermatology field. Such adjuvants are introduced in amounts generally used in the cosmetic or dermatological arts. In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin improving effect.

The cosmetic composition according to the present invention may include other ingredients in addition to the above-mentioned substances within the range not impairing the main effect, preferably giving a synergistic effect to the main effect. In addition, the cosmetic composition according to the present invention may further include a moisturizer, an emulsifier, a UV absorber, a preservative, a fungicide, an antioxidant, a pH adjuster, organic and inorganic pigments, flavors, coolants or limiting agents. The blending amount of the above components can be easily selected by those skilled in the art within the range that does not impair the object and effect of the present invention, the blending amount may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight based on the total weight of the composition. have.

In a composition that is one aspect of the invention, the composition comprises a pharmaceutical composition.

When the composition according to the present invention is applied to medicines, the composition may be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding a commercially available inorganic or organic carrier.

Examples of preparations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups, pellets and the like. In addition, preparations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, patches, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method, and surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffers, suspensions, and other commonly used auxiliaries can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenteral, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal, subcutaneous.

The active ingredient of the pharmaceutical composition of the present invention will depend on the age, sex, weight, pathology and severity of the subject to be administered, the route of administration or the judgment of the prescriber. Dosage determination based on these factors is within the level of one skilled in the art and its daily dosage may be, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / kg. May be, but is not limited to.

In a composition which is one aspect of the present invention, the composition comprises a health food composition.

The composition according to the invention provides various types of food additives or functional foods comprising. Fermented milk, cheese, yogurt, juice, probiotic, tablets, granules, drinks, caramels, diet bars and the like containing the composition, and can be processed into conventional tea leaf form or tea bags and dietary supplements. And other various food additives.

In one embodiment, the composition may contain other ingredients and the like that can give a synergistic effect to the main effect within a range that does not impair the main effect of the present invention. For example, it may further include additives such as perfumes, pigments, fungicides, antioxidants, preservatives, moisturizers, thickeners, inorganic salts, emulsifiers and synthetic polymer materials to improve physical properties. In addition, supplementary ingredients such as water soluble vitamins, oil soluble vitamins, polymer peptides, polymer polysaccharides and seaweed extract may be further included. The components may be appropriately selected and blended by those skilled in the art according to the formulation or purpose of use, and the amount of the additives may be selected within a range that does not impair the object and effect of the present invention. For example, the addition amount of the components may be in the range of 0.01 to 5% by weight, more specifically 0.01 to 3% by weight, based on the total weight of the composition.

The formulations of the compositions according to the invention may be in various forms, such as solutions, emulsions, viscous mixtures, tablets, powders, and the like, which may be administered by various methods such as simple drinking, injection, spray or squeeze.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

[ Production Example  1] Preparation of Bean Pod Extract

1.5 kg of bean pods were pulverized with a blender, and 7 L of an 80% ethanol aqueous solution was added thereto, followed by extraction under reflux three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 252 g of bean pod extracts.

[ Comparative example  1] Preparation of Soybean Extract

1.5 kg of beans were pulverized with a blender, and 7 L of an 80% ethanol aqueous solution was added thereto, followed by extraction under reflux three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 343 g of a soybean extract.

[ Test Example  1] improvement of inflammation

1. Inhibitory Effect of Prostaglandin Production

The anti-inflammatory effect was evaluated as the inhibitory effect of the production of prostaglandins. Soybean pod extract was used to measure the effect on macrophages. First, aspirin was added to macrophages taken from the abdominal cavity of mice to a final concentration of 500 M, thereby irreversibly inhibiting cyclooxygenase (COX) activity remaining in the cells. Then, the suspension was added to each well of a 96-well cell culture tube and incubated for 2 hours in an incubator at 37 ° C. with 5% CO ₂ to attach macrophages to the container surface. Subsequently, the attached macrophages were washed three times with PBS and then used for the inflammation improvement effect test.

The cultured macrophages 5x10 ⁴ cells / ml by adding RPMI medium containing 1% (w / v) of LPS incubated for 12 hours to induce the production of prostaglandins and 100μl treated with soybean pod extract and soy extract Free prostaglandins were quantified using enzyme immunoassay (ELISA).

At this time, the production inhibitory activity of prostaglandins of soybean pod extract and soybean extract was set to 100% of the difference between the prostaglandin produced in the LPS-treated and untreated groups, and the LPS and the sample were treated to reduce the production of prostaglandin. The results were compared with the control group to determine the result, and the results (prostaglandin production inhibitory effect) are shown in Table 1 below.

Test substance Prostaglandin inhibitory activity (%) Blank 100% Control group (aspirin treated group) 25.0% Bean pod extract 21.8% Bean extract 15.7%

As shown in Table 1, it can be seen that even when treated with the bean pod extract like the control group treated with aspirin, the effect of inhibiting the production of prostaglandins is very high. In addition, it can be seen that the bean pod extract has a superior inhibitory effect on the production of prostaglandins as compared to the soybean extract. It can be seen that the bean pod extract of the present invention can provide an excellent inflammation improving effect. In addition, it can be seen that the bean pod extract can prevent and improve skin trouble by inhibiting the expression of prostaglandin, a skin inflammatory factor.

2. IL -8 Production Suppression Effect

One day before the experiment, normal human skin keratinocytes (NHEK, obtained from Lonza) were dispensed in 96-well plates at 5x10 ⁴ cells / well, and then in a 37 ° C, 5% CO ₂ incubator. Incubated for 24 hours. After 24 hours, the cells were washed twice with PBS and changed to serum-free keratinocyte basement media (KBM). Each well was treated with soybean pod extract and soybean extract according to the concentrations of Table 2, followed by reaction for 30 minutes, followed by PGSA (10 µg / ml), PGSA (50 µg / ml), and PGSA (50 µg / ml) + LPS. (1 μg / ml) was treated respectively. Here, PGSA (peptidoglycan from S. aureus ) is a peptidoglycan (peptidoglycan) extracted from staphylococcus aureus, a major component of the Gram-positive (+) cell wall and bacterial membrane components are known to cause inflammation, In particular, in staphylococci, about 90% of patients with atopic dermatitis have been reported to cause secondary infection. Lypopolysaccaride (LPS) is a major component of the cell membrane of Gram-negative bacteria and is known to be a major cause of inflammation.

After incubating for 24 hours at 37 ° C., 5% CO ₂ incubator, the culture medium was taken and subjected to ELISA for Interleukin-8 (IL-8), and the results are shown in Table 2 below. ELISA used the experimental method of the manufacturer (BD science).

Test substance IL-8 secretion (pg / ml) Untreated Control (Control) 935.12 PGSA (10 μg / ml) 4812.60 PGSA (50 μg / ml) 5895.08 PGSA (50 μg / ml) + LPS (1 μg / ml) 6814.91 Bean Sprout Extract (5ppm) 1563.25 Bean Sprout Extract (25ppm) 1103.29 Bean Sprout Extract (50ppm) 1003.57 Soybean Extract (5ppm) 2970.18 Soybean Extract (25ppm) 2096.25 Bean Extract (50ppm) 1906.78

In Table 2, it was confirmed that the beans pod extract significantly reduced and inhibited the secretion of IL-8 increased by PGSA and LPS. Therefore, it can be seen that the topical skin composition of the present invention can provide an excellent anti-inflammatory effect by significantly reducing the secretion of IL-8 increased by PGSA and LPS.

[ Test Example  2] itching relief assessment

One day before the experiment, the keratinocytes (Cell name: HaCaT obtained from ATCC) were dispensed into 96 well plates at 4x10 ⁴ cells / well, followed by incubation for 24 hours in a 37 ° C, 5% CO ₂ incubator. It was. After 24 hours, wash 96 well plates twice with Hanks'Balanced Salt solution (HBSS) buffer, and then add reaction buffer (2 μM Fluo-4-AM, 20% pluronic acid, 2.5 mM probenecid) to the cells. gave. After reaction at 37 ° C., 5% CO ₂ incubator for 30 minutes, and at room temperature for 30 minutes, the cells were washed twice with HBSS buffer and treated with soybean pod extract and soybean extract at the concentrations (%) shown in Table 3 below.

After reaction for 10 minutes to process the 2U / ml trypsin (Trypsin) or 5μM PAR-2 activation peptide (SLIGKV) and was measured in Ca ^{2 +} concentration in the cell 80 seconds. In Ca ^{2 +} concentration in the measurement cell presentation flex 3: was used (FlexStation 3 Molecular Device, USA) . After the soybean pod extract or soybean extract and 2U / ml Trypsin or 5μM PAR-2 active peptide (SLIGKV) treatment, the flex was measured for 80 seconds to determine the difference between the minimum and maximum values. The percent inhibition against intracellular influx of calcium ions compared to the difference between the minimum and maximum values treated with 2U / ml trypsin or 5 μM PAR-2 active peptide (SLIGKV) is shown in Table 3 below.

Test substance concentration % Inhibition of intracellular influx of calcium ions Trypsin (2U / ml) PAR-2 active peptide (5 μM) Bean Pod Extract 0.05% 26 28 0.1% 31 34 0.5% 40 42 1.0% 46 50 Bean extract 0.05% 10 11 0.1% 12 14 0.5% 16 17 1.0% 18 20

As can be seen in Table 3, the influx of intracellular calcium ions by trypsin or PAR-2 active peptide (SLIGKV) is reduced by the treatment of soybean pod extract, and as the concentration of soybean pod extract increases It was confirmed that the influx of them was significantly reduced. In addition, it can be seen that the efficacy of the bean pod extract is superior to the soybean extract.

Therefore, the external preparation composition for skin containing the bean pod extract of the present invention can provide an excellent anti-pruritic effect by effectively inhibiting PAR-2 activity causing itching.

[ Test Example 3 Atopic Symptom Improvement Assessment

In order to use the composition according to the composition of the present invention as a cosmetic, a composition containing a bean pod extract was prepared with a cream with the composition specified in Table 4 below.

Compounding Ingredients (wt%) Formulation Example 1 Comparative Formulation Example 1 Comparative Formulation Example 2 Purified water To 100 To 100 To 100 Bean pod extract 0.1 - - Bean extract - 0.1 - Vegetable Cured Oil 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 Stearyl alcohol 2.00 2.00 2.00 Glycerol Stearate 1.00 1.00 1.00 Polyglyceryl-10 Pentastearate &
Behenyl Alcohol & Sodium Stearoyl Lactylate 1.00 1.00 1.00 Arachidil Behenyl Alcohol & Arachidil Glucoside 1.00 1.00 1.00 Cetylaryl Alcohol & Cetearyl Glucoside 2.00 2.00 2.00 PEG-100 Stearate & Glycerol Olate & Propylene Glycol 1.50 1.50 1.50 Caprylic / Capric Triglycerides 11.00 11.00 11.00 Cyclomedicon 6.00 6.00 6.00 Triethanolamine 0.1 0.1 0.1

In order to confirm the effect of improving atopic symptoms by the composition of the present invention, the cream prepared in Formulation Example 1 and Comparative Formulation Examples 1 and 2 was applied to the panels to evaluate the degree of atopic dermatitis improvement. For 15 panelists who had symptoms of dermatitis showing similar symptoms, divided into 5 groups of 5 people each, and each composition of Table 6 below twice daily for 12 weeks at a temperature of 24 to 26 ° C and a humidity of 75% for 12 weeks. It was applied to the face. At the end of the study, subjects were asked to complete a questionnaire and subjective efficacy evaluations were conducted. The questionnaire included symptoms of atopic dermatitis such as "pruritus", "dryness", "keratogenesis", "dandruff", "erythema", "swelling", "skin cracking", "duty and eczema", and "tertiary disease". After dividing the above symptoms, the degree of improvement for each symptom was evaluated and averaged to evaluate the atopic symptoms. The results are shown in Table 5.

Application group (number of the respondents-person) Formulation Example 1 Comparative Formulation Example 1 Comparative Formulation Example 2 Markedly improved 4 Improving One 2 One No change 3 5 worse

From Table 5, it can be seen that the composition containing the bean pod extract of the present invention further improves the existing atopic dermatitis symptoms compared to Comparative Formulation Example 3. Also, compared to the Comparative Formulation Example 2 containing soybean extract It can be seen that the composition containing the pod extract shows a more excellent improvement effect.

[ Example  6] skin moisturizing test

When human keratinocytes cultured in primary culture were put in a culture flask and attached to the bottom, and then the bean pod extract and soybean extract were added to the culture solution at a concentration of 1 mg / ml, and the cells grew about 80 to 90% of the floor area. Incubated for 5 days. The cells were harvested and washed with PBS (phosphate buffered saline) and then 10 mM Tris-HCl (Tris-HCl, pH 7.4) containing 2% Sodium Dodecyl Sulfate (SDS) and 20 mM Dithiothreitol (DTT). 1 ml was added and sonicated for 3 minutes, and then boiled for 10 minutes. Thereafter, the mixture was centrifuged at 1200 rpm for 30 minutes, the precipitate was separated, suspended in 1 ml of PBS, and the absorbance was measured at 340 nm.

Separately, a portion of the solution after the sonication was taken to measure the protein content, which was used as a reference for evaluating the degree of cell differentiation. Low calcium (0.03mM) treated group and high calcium (1.2mM) treated group as a negative / positive control, respectively, the test results performed by adding the test substance to the low calcium concentration is shown in Table 6 below.

Test substance Concentration Differentiation capacity in keratinocytes (%) Control Low Ca ^{2 +} (0.03 mM) 100 Control High Ca ^{2 +} (1.2 mM) 207 Bean extract 1 mg / ml 131 Bean pod extract 1 mg / ml 191

As shown in Table 6 above, by measuring the amount of CE (Cornified Envelop) produced when keratinocyte differentiation, and comparing the effect of promoting cell differentiation, Soybean pod extract according to the present invention was found to promote differentiation in keratinocytes compared to soybean extract.

Hereinafter, the formulation examples of the composition according to the present invention, but the pharmaceutical composition and cosmetic composition is applicable to a variety of formulations, which is intended to explain in detail only, not intended to limit the present invention.

Preparation Example 1 Soft Capsule

Soybean pod extract 150 mg, palm oil 2 mg, palm hardened oil 8 mg, lead 4 mg and lecithin 6 mg were mixed and 400 mg per capsule was prepared according to a conventional method to prepare a soft capsule.

Preparation Example 2 Tablet

Bean pod extract 150 mg, glucose 100 mg, red ginseng extract 50 mg, starch 96 mg and magnesium stearate 4 mg were mixed and 30 mg of ethanol was added to form granules, and then dried at 60 ° C. using a tablet press. Tableting.

Preparation Example 3 Granule

Soybean pod extract 150 mg, glucose 100 mg, red ginseng extract 50 mg and starch 600 mg were mixed and granules were formed by adding 100 mg of 30% ethanol, dried at 60 ° C. to form granules, and then filled into sachets. The final weight of the content was 1 g.

[Example 4] Drinks

Bean pod extract 150 mg, glucose 10 g, red ginseng extract 50 mg, citric acid 2 g and purified water 187.8 g was mixed and filled with a bottle. The final dose of the contents was 200 ml.

Preparation Example 5 Preparation of Health Food

Bean pod extract ............................. 1000 mg

Vitamin mixtures

Vitamin A Acetate ......... 70 μg

Vitamin E ......................................... 1.0 mg

Vitamin B1 ..................... 0.13 mg

Vitamin B2 ......................................... 0.15 mg

Vitamin B6 ......................................... 0.5 mg

Vitamin B12 ......................... 0.2 μg

Vitamin C ......................................... 10 mg

Biotin ... 10 ㎍

Nicotinic Acid Amide ...................................... 1.7 mg

Folic Acid ... ..... 50 ㎍

Calcium Pantothenate ............... 0.5 mg

Mineral mixture

Ferrous Sulfate ......................................... 1.75 mg

Zinc Oxide ......................................... 0.82 mg

Magnesium Carbonate ......................................... 25.3 mg

Potassium monophosphate ......................................... 15 mg

Dibasic Calcium Phosphate ......................................... 55 mg

Potassium Citrate ......................................... 90 mg

Calcium Carbonate ......................................... 100 mg

Magnesium Chloride ......................................... 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food composition according to a conventional method.

Preparation Example 6 Preparation of Healthy Drinks

Soybean pod extract ............................ 1000 mg

Citric Acid ... .... 1000 mg

oligosaccharide................................................. .... 100 g

Plum concentrate ..................... ..... 2 g

Taurine ... ........... 1 g

Purified water is added to the whole ........... 900 ㎖

After mixing the above components according to a conventional healthy beverage production method, and then stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained by sterilization in a sterilized 2 L container, sealed sterilization and then refrigerated and stored in the present invention For the preparation of healthy beverage compositions.

Although the composition ratio is mixed with a component suitable for a favorite beverage in a preferred embodiment, the compounding ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and use purpose. Those skilled in the art to which the present invention pertains will be able to perform various applications and modifications within the scope of the present invention based on the above contents.

Formulation Example 2 Softening Cosmetic (Skin Lotion)

Compounding ingredient Content (weight%) Bean pod extract 0.1 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 Fiji-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation Example 3 Nutritious Longevity (Milk Lotion)

Compounding ingredient Content (weight%) Bean pod extract 0.1 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Beeswax 4.0 Polysorbate 60 1.5 Caprylic / Capric Triglycerides 5.0 Squalane 5.0 Sorbitassquioleate 1.5 Liquid paraffin 0.5 Cetearyl Alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation Example 4 Nutrition Cream

Compounding ingredient Content (% by weight) Bean pod extract 0.1 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Squalane 5.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation 5 Massage Cream

Compounding ingredient Content (% by weight) Bean Pod Extract 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Beeswax 4.0 Cetearyl Glucoside 1.5 Sesqui oleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, flavoring Quantity Purified water Remaining amount

[Formulation Example 6] Pack

Compounding ingredient Content (% by weight) Bean pod extract 0.1 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl Phenyl Ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 quantity Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation Example 7 Ointment

Compounding ingredient Content (% by weight) Bean pod extract 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Squalane 1.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Cetearyl Alcohol 1.0 Beeswax 4.0 Preservative, coloring, flavoring Quantity Purified water Remaining amount

As described above in detail the specific parts of the present invention, it is apparent to those skilled in the art that such specific description is merely a preferred embodiment, thereby not limiting the scope of the present invention. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (11)

delete Moisturizing composition comprising soybean pod extract as an active ingredient. The composition of claim 2, wherein the composition is for preventing or improving atopic dermatitis. delete The composition of claim 2 or 3, wherein the composition comprises from 0.001 to 10% by weight of the bean pod extract based on the total weight of the composition. According to claim 2 or 3, wherein the soybean (Seoritae, Glycin max MERR), Seomoktae (Seomoktae, Rhynchosia Nolubilis ), Black soybean, Glycine max (L.) Merr., Blue bean, Glycime max MERR, yellow bean, Glycime max MERR, field bean, Vicia faba , kidney bean (kidney bean, Phaseolus vulgaris ), pinto bean ( Phaseolus vulgaris L.), red (small red) bean, Vigna angularis ), soybeans (small black bean, Phaseolus angularis WF WIGHT.), bean sprouts (sprouting bean, Glycine max (L.) Merr.) and soybeans (soybean, Glycine max ) The above composition. The composition of claim 2 or 3, wherein the composition inhibits or inhibits the production of prostaglandins. The composition of claim 2 or 3, wherein the composition inhibits or inhibits the production of interleukin. The composition of claim 2 or 3, wherein the composition is a cosmetic composition. The composition of claim 2 or 3, wherein the composition is a pharmaceutical composition. The composition of claim 2 or 3, wherein the composition is a health food composition.