KR100902326B1 - Copmosition comprising extract of germinated soybean - Google Patents

Abstract

The present invention relates to a composition for preventing or treating acne, a composition for preventing or treating inflammatory diseases and an antimicrobial composition comprising a germinated bean extract. More specifically, the composition comprising the germinated soybean extract of the present invention is excellent in anti-inflammatory effect, antimicrobial effect, in particular, acne and antioxidant effect, the composition comprising the germinated soybean extract and magnolia bark extract of the present invention together It is excellent in controlling sebum secretion, inhibiting hyperkeratosis, suppressing and alleviating scalp formation, and preventing and treating acne.

Germinated beans, Magnolia bark, Acne, Magnolol, Honokiol

Description

Composition comprising germinated bean extract {COPMOSITION COMPRISING EXTRACT OF GERMINATED SOYBEAN}

The present invention relates to a composition for preventing or treating acne, a composition for preventing or treating an inflammatory disease, and an antimicrobial composition comprising a germinated soybean extract, and more particularly, having an excellent anti-inflammatory effect and an antimicrobial effect, in particular, an anti acne and anti-oxidant effect. It relates to a composition comprising a germinated soybean extract excellent in effect.

Factors that threaten the safety of various agricultural products or foods include harmful microbial contamination, chemical contamination such as pesticides, and environmental pollution. Although the most serious problem has emerged, it has not been solved yet, and the cause of disease infection in animals including humans relates to harmful microbial contamination.

The microorganisms include Escherichia coli (E. coli), Salmonella typhimurium (Salmonella typhimurium), hwanonggyun i.e., Staphylococcus aureus (Staphyllococcus aureus) and fungi such as Candida related, especially skin diseases that cause infections in animals, including harmful sikjungdokgyun the person (Candida albican ), Earth fungus ( Aspergillus) niger ) or Propionibacterium acnes ) and the like.

In particular, the processed food or agricultural and aquatic products are distributed and consumed on a large scale, and if they are contaminated by harmful bacteria due to unsanitary treatment during processing, transportation and storage, there is a high possibility of causing food poisoning accidents due to their growth. In addition, due to the development of transportation and modernized living environment, such as dense residential area, as the contact with a large number of people is increased, the possibility of transmission of various skin disease-causing bacteria is amplified, thereby increasing the possibility of infection of various diseases.

Therefore, in order to secure the stability of the food from the harmful microorganisms as described above, various kinds of antimicrobial agents are used together with the existing heat treatment and low temperature treatment methods, and the like or cosmetics containing various kinds of antimicrobial compositions for the treatment of skin diseases. Compositions and the like have been developed and are commercially available.

However, since most antimicrobial agents currently on the market contain chemically synthesized chemical components as main ingredients, they may damage humans or animals with toxic substances depending on the content of the ingredients or the conditions of use. It is required to develop an alternative antimicrobial composition that has the same antimicrobial activity as a chemical component and is derived from natural plants to ensure safety.

Inflammation protects the living body as a locally occurring immune response when tissue is damaged or destroyed by external harmful substances or organisms, such as bacteria, fungi, viruses, or various types of allergens. It is a useful defense mechanism to remove the products produced by tissue damage.

In normal cases, the organism restores normal structure and function by neutralizing or removing the pathogens and regenerating the damaged tissues through inflammatory reactions, but the inflammatory reaction is excessive or persistent due to the absence of antigens or internal substances. Rather, it becomes a major pathology of the disease (such as irritable disease or chronic inflammation), and has been reported to be an obstacle in the treatment process such as blood transfusion, drug administration, and organ transplantation. In addition to being able to observe the inflammatory response in almost all diseases, it is known that enzymes related to the inflammatory response play an important role in carcinogenesis.

Various biochemical phenomena are involved in the development of inflammation in the living body, and particularly, nitric oxide synthase (NOS) and prostaglandin, enzymes that generate nitric oxide (NO), are involved. Enzymes related to biosynthesis are known to play an important role in mediating the inflammatory response, thus synthesizing prostaglandins from NOS, an enzyme that produces NO from L-Arginine, or Arachidonic acid. COX (cyclooxygenase), an enzyme that is involved in the detoxification process, is a major target in blocking inflammation.

The anti-inflammatory effects of most nonsteroidal antiinflammatory drugs (NSAIDs) are mediated by inhibition of cyclooxygenase (COX) enzyme activity (Vane et al., Annu. Rev. Pharmacol. Toxicol., 38, 97, (1998). )). The COX is a major enzyme involved in the biosynthesis of prostaglandin present in vivo (Smith et al., J. Biol. Chem., 271, 33157 (1996)), and the COX is a two-type isozyme, COX- 1 and COX-2 are reported. The COX-1 is constantly present in tissues such as the stomach and kidneys, and is involved in maintaining normal homeostasis, while the COX-2 is involved in mitogen or cytokines during inflammation or other immune responses. Is an enzyme that is transiently and rapidly expressed in cells.

Nonsteroidal anti-inflammatory compositions used for the treatment of chronic inflammatory diseases such as acute or rheumatoid arthritis are known to exhibit side effects such as gastrointestinal disorders by inhibiting COX-2 enzymes as well as COX-1 enzymes. Masferrer et al., Proc. Natl. Acad. Sci. USA, 91, 3228, 1994; Seibert et al., Proc. Natl. Acad. Sci. USA, 91, 12013, 1994). In addition, most NSAIDs (non-steroidal anti-inflammatory compositions) currently used as therapeutic agents cause side effects such as gastrointestinal disorders, liver disorders, and renal dysfunction, and thus are difficult to use for a long time, thus increasing the efficacy of anti-inflammatory drugs and minimizing side effects. In order to selectively inhibit only COX-2 enzyme, it is required to develop a new anti-inflammatory analgesic agent with fewer side effects that can be used for a long time as a treatment for systemic chronic diseases.

Recently, NS-398, CGP-29238, SC-58125, L-745337, meloxicam, etc. have been reported as inhibitors for COX-2 produced by synthetic methods, but they are not widely used. The side effects are not well proven.

Therefore, there is an increasing demand for the development of anti-inflammatory analgesic compositions derived from natural plants and the like that can be used for a long time because stability and side effects are not a problem.

In general, the occurrence of acne is known to be caused by various causes, in particular, the secretion of sebum due to the action of hormones, keratinocyte hyperkeratosis in sebaceous gland and acne bacteria ( Propionibacterium) Acnes ) is reported to be caused by a combination of three factors: involvement.

Specifically, the inner wall of the sebaceous gland becomes thick due to abnormal hyperkeratosis of the sebaceous gland in the sebaceous gland, and thus the sebaceous glands are blocked, and as sebum secretion is increased, the sebum produced is continuously enclosed in the sebaceous gland. It is reported to promote acne outbreaks. In addition, as acne bacteria proliferate in sebaceous glands, triglyceride constituting sebum is converted into free fatty acids and acts as irritants, or is induced by cotton acne. It acts as a comedogenic substance to promote acne production. In addition, if the above acne-producing mechanism is further progressed, it may be accompanied by an inflammatory reaction (Sergio Nacht, Cosmetics Toiletries, 101, 47-55, 1986; Wei-Li Lee et al., Infection and Immunity, 35, 71- 78, 1982).

Therefore, the evaluation of products for the purpose of alleviating acne has been carried out by using the above-described mechanisms of action such as suppressing hyperkeratosis, controlling sebum secretion, and bactericidal activity against acne bacteria (Madli Puhvel, Cosmetics Toiletries). , 98, 117-119, 1983; Kathy Perisho et al., J. of Invest.Dermatol., 90, 350-353, 1988; Hyeyoung Kim et al., Korean J. Ginseng Sci., 14, 391-398, 1990; RA Bojar Et al., British J. of Dermatology, 132, 204-208, 1995; AM Kligman et al., British J. of Dermatology, 100, 699-702, 1979).

In order to treat such acne, attempts have been made to have a therapeutic effect by smoothing the secretion of sebum through exfoliation of pores using salicylic acid or vitamin A derivatives, or by using benzoyl peroxide. Attempts have been made to cure through removal and strong antimicrobial action. However, salicylic acid preparations can cause skin redness, swelling or skin repellency, as well as poor therapeutic effects, while retinoic acid preparations have the effect of inhibiting hyperkeratosis, but contact dermatitis, erythema, skin drying and peeling may occur. In addition, many side effects have been reported in benzoyl peroxide formulations, such as allergic contact dermatitis, scarring and severe erythema.

In view of these problems, the present inventors have studied to develop substances from natural plants which have excellent antibacterial activity, excellent anti-inflammatory effect, excellent prevention and treatment effect of acne, and excellent safety for human body even after long time use. As a result, the composition containing the germinated soybean extract has excellent antibacterial activity, excellent anti-inflammatory effect, excellent prevention and treatment effect of acne, and excellent antioxidant effect, When using magnolias bark extract (magnolias bark extract), it was confirmed that the excellent effect and the excellent safety was completed the present invention.

In order to solve the problems of the prior art, an object of the present invention is to provide an antimicrobial composition comprising a germinated soybean extract having an antimicrobial activity against harmful microorganisms, in particular, acne bacteria as an active ingredient.

It is another object of the present invention to provide an anti-inflammatory composition comprising a germinated bean extract.

Still another object of the present invention is to provide a composition for preventing and treating acne, including the germinated bean extract as an active ingredient. The composition for preventing and treating acne containing the germinated soybean extract is preferably an excellent effect on the sebum secretion control effect, hyperkeratosis inhibitory effect, vesicle formation inhibitory and alleviation effect, acne prevention and treatment, in addition to the germinated bean extract Magnolol and Honokiol (honokiol) may be to include the extract of the bark of the main ingredient.

The acne treatment and prevention composition may be used as a medical, food, or cosmetic composition.

In order to achieve the above object, the present invention provides an antimicrobial composition comprising a germinated soybean extract having an antimicrobial activity against harmful microorganisms, in particular, acne bacteria as an active ingredient.

The present invention also provides an anti-inflammatory composition comprising a germinated bean extract.

The present invention also provides a composition for preventing and treating acne comprising a germinated bean extract as an active ingredient. The composition for preventing and treating acne may further include a magnolia bark extract mainly containing magnolol and honokiol.

The acne treatment and prevention composition may be used as a medical, food, or cosmetic composition.

Hereinafter, the present invention will be described in more detail.

Germinated beans in the present invention is a germination of a year-old herb beans belonging to the legumes. The beans are not particularly limited, including all commonly known varieties. Specifically, the soybean may be frosted, peas, green beans, white kidney beans, red kidney beans, black beans, Seomoktae, bal beans, cheongtae, etc., preferably may be meju beans or black beans, more preferably black beans.

The germinated soybean germination of the soybean sprout may be grown to about 0.5 cm to 1 cm, but the present invention is not limited to the length of the shoot of the bean. Specifically, as the germinated washed beans, the germinated beans or extracts of germinated beans contain various amino acids, minerals, enzymes, vitamins, isoflavones. In addition, the production method of the germinated bean and its use in food are mainly known.

The germinated bean may be prepared by performing a immersion step and a germination step for the soybeans, but the method for producing the germinated soybeans is not particularly limited. In addition, the germinated soybeans may further perform a drying step after performing the immersion step and the germination step.

The immersion step is 12 hours to 72 hours, preferably 16 hours to 60 hours, more preferably at 10 ℃ to 35 ℃, preferably 15 ℃ to 30 ℃, more preferably 20 ℃ to 30 ℃ using purified water. Preferably soaked for 24 to 48 hours. The purified water may be used 5 times to 20 times, preferably 10 times to 15 times water based on the mass of the germinated beans.

In addition, the germination step is 24 to 144 hours, preferably 48 to 120 hours, more preferably at 10 ℃ to 35 ℃, preferably 15 ℃ to 30 ℃, more preferably 20 ℃ to 30 ℃ 72 hours to 96 hours.

More specifically, the germinated soybeans are put into 10 times the purified water in the washed soybean or black soybeans soaked at 20 ℃ to 30 ℃ for 24 hours to 48 hours, then the soaked beans are picked up at 20 ℃ to 30 ℃ Germination for 72 to 96 hours can be prepared via a step.

Germinated bean extract of the present invention may be prepared according to a conventional method for producing a plant extract.

The germinated soybean extract may be prepared by extracting and / or fractionating a part or all of the germinated soybean with an extraction solvent and / or a fractional solvent, for example, a method of obtaining an extract after adding a solvent to the germinated soybean or its pulverized product. It can be manufactured by.

The extraction solvent may be water or an organic solvent such as a polar solvent such as an alcohol having 1 to 5 carbon atoms, ethyl acetate, a nonpolar solvent such as hexane or dichloromecan, or a mixed solvent thereof, and the alcohol may be ethanol, methanol, butanol, Propanol and isopropanol may be, but are not limited thereto. Preferably, the extraction solvent may be any one selected from the group consisting of water, alcohols having 1 to 5 carbon atoms, propylene glycol and 1,3-butylene glycol, more preferably water or 1,3-butylene glycol It may be, and most preferably may be 1,3-butylene glycol.

Germinated soybean extract of the present invention may be prepared according to a conventional method for producing a plant extract, specifically may be cold extraction, hot extraction or heat extraction, etc., preferably cold extraction, may be a conventional extraction device , Ultrasonic grinding extractor or fractionator can be used. The extraction temperature of the cold extraction method may be carried out at room temperature, preferably 10 ° C to 25 ° C, more preferably 15 ° C to 20 ° C.

Extraction time of the cold extraction method is not limited, but may be specifically 24 hours to 120 hours, preferably 48 hours to 96 hours, more preferably 60 hours to 80 hours.

In addition, the extract extracted with the extraction solvent may be further subjected to the fractionation process with a solvent selected from the group consisting of hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform, water and mixtures thereof. One or more solvents may be used in the fractionation. Fraction of the extract may be carried out at 4 ℃ to 120 ℃, but is not limited thereto.

The prepared extract or fraction may then be removed by filtration or concentration or drying to remove the solvent, it can be carried out both filtration, concentration and drying. Specifically, the filtration may be performed using a filter paper or a filter or a reduced pressure filter, and the concentration may be concentrated under reduced pressure using a reduced pressure concentrator, for example, a rotary evaporator, and the drying may be performed by, for example, a lyophilization method.

In a specific example of the present invention, the germinated soybean extract manufacturing method can be carried out by immersing the germinated soybeans in a common solvent of distilled water and 1,3-butylene glycol soaked for 3 days and then filtered using a filter. have

In addition, the magnolia bark extract of the present invention may be prepared according to a conventional method for producing a plant extract.

The magnolia bark extract may be prepared by extracting and / or fractionating some or all of the magnolia bark with an extraction solvent and / or a fraction solvent, for example, a method of obtaining an extract after adding a solvent to the magnolia bark or its pulverized product. It may be prepared as.

The magnolia bark extract is composed mainly of magnolol of formula (1) or honokiol of formula (2). Magnolol and Honokiol, which are the main components, are generally present at 0.05% to 5.0% in the extract of magnolia bark, and are reported to have anti-inflammatory effects, skin cancer suppression effects, and antibacterial effects.

The extraction solvent used in the extract of magnolia bark may be an organic solvent such as water or a polar solvent such as an alcohol having 1 to 5 carbon atoms, ethyl acetate, a nonpolar solvent such as hexane or dichloromecan, or a mixed solvent thereof. Ethanol, methanol, butanol, propanol and isopropanol may be, but are not limited to. Preferably, the extraction solvent may be any one selected from the group consisting of water, alcohol having 1 to 5 carbon atoms, propylene glycol and 1,3-butylene glycol, more preferably propylene glycol or 1,3-butylene It may be glycol.

Magnolia bark extract of the present invention may be prepared according to a conventional method of producing a plant extract, specifically may be cold extraction, hot extraction or heat extraction, etc., preferably cold extraction, conventional extraction equipment , Ultrasonic grinding extractor or fractionator can be used. Extraction time of the cold extraction method is not limited, but may be specifically 12 hours to 120 hours, preferably 24 hours to 96 hours, more preferably 36 hours to 60 hours. The extraction method of the present invention is not limited to the above extraction method, and a method known to those skilled in the art to which the present invention belongs may be applied.

In addition, the extract extracted with the extraction solvent may be further subjected to the fractionation process with a solvent selected from the group consisting of hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform, water and mixtures thereof. One or more solvents may be used in the fractionation. Fraction of the extract may be carried out at 4 ℃ to 120 ℃, but is not limited thereto.

The prepared extract or fraction may then be removed by filtration or concentration or drying to remove the solvent, it can be carried out both filtration, concentration and drying. Specifically, the filtration may be performed using a filter paper or a filter or a reduced pressure filter, and the concentration may be concentrated under reduced pressure using a reduced pressure concentrator, for example, a rotary evaporator, and the drying may be performed by, for example, a lyophilization method.

In a specific embodiment of the present invention, the method for preparing the magnolia bark extract may be performed by filtration of the magnolia bark by immersing the magnolia bark in a common solvent of 1,3-butylene glycol for 3 days and then using a filter.

Germinated soybean extract of the present invention was confirmed to have a strong antimicrobial effect even when a small amount applied to harmful microorganisms, in particular, acne bacteria, anti-inflammatory and antioxidant effects, in particular, the introduction of germinated soybean extract as an anti-inflammatory and antioxidant to acne skin When it was confirmed that the anti-inflammatory and antioxidant effect is very excellent, when the three effects of the anti-inflammatory, antioxidant, and antibacterial to the acne skin at the same time, it was found that the most effective.

As a result of the present inventors confirmed that the results, in particular, it is effective to simultaneously apply antibacterial raw materials resistant to acne bacteria to acne bacteria and anti-inflammatory and antioxidant effects to acne treatment and prevention products, acne skin mostly acne bacteria It may be because they are weak and easily infected, and usually have inflammation.

The present invention provides an antimicrobial composition comprising a germinated bean extract.

In the present invention, the antimicrobial composition may have the same meaning as an antibiotic which is a generic term for an antimicrobial agent, and may have the same meaning as an antimicrobial agent, a fungicide, a preservative, a preservative or an antimicrobial agent, and preferably the development of Gram-positive bacteria and Gram-negative bacteria. Means a substance that can inhibit or inhibit life and life functions.

The antimicrobial composition of the present invention may include 0.001% by weight to 99.99% by weight, preferably 0.1% by weight to 99% by weight, based on the total weight of the composition. According to the content of the active ingredient can be adjusted appropriately.

The antimicrobial composition is a pathogenic microorganism, specifically E. coli (H. coli), Salmonella typhimurium (Salmonella typhimurium), Staphyllococcus aureus ), Candida albican ), Earth fungus ( Aspergillus) niger ) or Propionibacterium acnes ), preferably Candida albican ), Earth fungus ( Aspergillus) niger ) or Propionibacterium acnes ), and more preferably Propionibacterium acnes ) may be an antimicrobial composition having antimicrobial activity.

The antimicrobial composition of the present invention can be applied directly to animals including humans to exhibit antimicrobial activity. The animal is a biological group corresponding to plants, mainly ingesting organic matter as nutrients, and means that digestion, embryogenicity, and respiratory organs are differentiated, specifically, echinoderm, crustaceans, molluscs, fish, amphibians, reptiles, birds, It may be a mammal, preferably mollusks such as shellfish such as sea urchins or sea cucumbers, crustaceans such as crabs, shrimps, and lobsters, mollusks such as cephalopods, gastropoda or bivalve shells, red snapper, sea bream, cod, flounder, It may be a bird including a fish such as a flounder, a poultry such as a pheasant or a chicken, or a mammal such as a human, a pig or a cow goat.

The antimicrobial composition of the present invention may include sugars, proteins, lipids, vitamins and minerals in addition to the germinated bean extract.

The sugar may be appropriately selected according to the purpose and use thereof, and may be, for example, honey, dextrin, sucrose, palatinose, glucose, fructose, syrup, sugar alcohol, sorbitol, xylitol, maltitol, It is not particularly limited thereto. The protein may be appropriately selected depending on the purpose and use thereof. For example, animal-derived enzymes such as milk derived proteins such as casein and whey protein, soy protein, trypsin of these proteins, and pepsin. And hydrolysates by neutrases and alkalases, but are not particularly limited thereto. The lipid may be appropriately selected according to the purpose and use thereof. For example, the monohydric saturated fatty acid, sunflower oil containing polyunsaturated fatty acid, rapeseed oil, olive oil, safflower oil, corn oil Oil, soybean oil, palm oil (palm oil), palm oil and other plant-derived fats, such as middle-chain fatty acids, EPA, DHA, soybean-derived phospholipids, milk-derived phospholipids, but is not particularly limited thereto. The vitamins are not particularly limited, and the minerals may be appropriately selected depending on the purpose and use thereof. For example, potassium phosphate, potassium carbonate, potassium chloride, sodium chloride, calcium lactate, calcium gluconate, calcium pantothenate, and casein calcium , Magnesium chloride, ferrous sulfate, sodium hydrogen carbonate, but is not particularly limited thereto.

For any of the above saccharides, proteins, lipids, vitamins and minerals, as long as the antimicrobial properties of the composition are maintained, not limited to the above embodiments and may include other ingredients, each content is not limited as long as the antimicrobial properties are maintained , Preferably 0.1 to 15% by weight, preferably 0.5 to 10% by weight relative to the total composition.

The antimicrobial composition of the present invention can be used for various purposes and uses requiring antimicrobial activity, specifically, the composition for the prevention or treatment of diseases caused by pathogenic microbial infections, cosmetic compositions, cosmetic additives, food, food additives, beverages, It may be used in the use of animal feed compositions, animal feed composition additives or detergents.

The present invention provides an anti-inflammatory composition comprising a germinated bean extract. The anti-inflammatory composition means a composition for the treatment or prevention of inflammatory diseases. Specifically, the anti-inflammatory composition of the present invention comprises a germinated soybean extract having an effect of inhibiting inflammation and selectively inhibiting expression of COX-2 as an active ingredient, and further comprising a pharmaceutically acceptable carrier, diluent or Provided are compositions for the treatment or prevention of inflammatory diseases comprising an excipient.

The inflammatory diseases include arteriosclerosis, rheumatoid arthritis, asthma, acute pain, chronic pain, neuropathic pain, postoperative pain, pains such as migraine and joint pain, neuropathy, nerve damage, irritable bowel syndrome, shock by endotoxin or Inflammatory bowel disease.

The anti-inflammatory composition of the present invention may include the germinated soybean extract as an active ingredient alone, and may further include a pharmaceutically acceptable carrier or excipient according to the formulation, method of use, and purpose of use. In the anti-inflammatory composition of the present invention, the germinated bean active ingredient may be used alone or in combination with a substance having other pharmacological activity as well as an appropriate set.

When provided in a mixture, the active ingredient may be included in the anti-inflammatory composition in an amount of 0.01 to 99.9% by weight, but usually in an amount of 0.1 to 50% by weight. The anti-inflammatory composition is intended for the prevention and treatment of various chronic inflammatory diseases such as rheumatoid arthritis, lupus, multiple sclerosis, sepsis, shock, various acute inflammatory diseases such as rejection of organ transplantation, ophthalmic diseases, bronchitis, or inflammatory bowel disease. Can be used as

Examples of the carrier or excipient include water, dextrin, calcium carbonate, lactose, propylene glycol, liquid paraffin, saline, dextrose, sucrose, sorbitol, mannitol, ziitol, erythritol, maltitol, starch, gelatin, calcium phosphate, Calcium silicate, cellulose, methyl cellulose, polyvinylpyrrolidone, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. Two or more carriers or excipients may be used. In addition, when the anti-inflammatory composition is formulated, conventional fillers, extenders, binders, disintegrants, surfactants, anti-coagulants, lubricants, wetting agents, fragrances, emulsifiers or preservatives may be further included.

In addition, the anti-inflammatory composition of the present invention may further include a compound or plant extract having a known anti-inflammatory activity in addition to the germinated bean extract, and may be included in an amount of 5 to 20 parts by weight based on 100 parts by weight of the germinated bean extract.

The formulation of the anti-inflammatory composition may be in a preferred form depending on the method of use, and in particular may be formulated by employing methods known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal. . Examples of specific formulations include warnings, granules, lotions, linings, limonades, powders, syrups, ointments, liquids, aerosols, EXTRACTS, elixirs, ointments, liquid extracts, emulsions, Suspending agents, premises, acupuncture, eye drops, tablets, suppositories, eye drops, spirits, capsules, creams, pills, soft or hard gelatin capsules.

The anti-inflammatory compositions according to the invention can be used orally or parenterally, such as by intradermal, intramuscular, intraperitoneal, intravenous, subcutaneous, nasal, epidural and oral routes, the dosages of which are The method of administration, the age, sex and weight of the recipient, and the severity of the disease may be determined. For example, the anti-inflammatory composition of the present invention is 0.0001 mg / kg (body weight) to 100 mg / kg (body weight) per day, preferably 0.01 mg / kg (body weight) to 10 mg per day based on the active ingredient It can be administered one or more times at / kg (body weight). However, the above dosage is only one example to illustrate and is not limited to the above range.

The anti-inflammatory composition of the present invention can be used as a nutraceutical or cosmetic composition comprising a medicament, the germinated soybean extract and a food supplement acceptable food additive, the anti-inflammatory composition is 0.001 to the pharmaceutical, nutraceutical or cosmetic composition To 50 wt%, but is not limited thereto.

Health functional foods to which the extract of the present invention may be added for the purpose of preventing inflammatory diseases include various foods, beverages, gum, tea, vitamin complexes, and health functional foods. The amount of the extract added to the food or beverage may be added at 0.01 to 15% by weight of the total food weight, the health beverage composition may be added at a rate of 0.02 to 5 g, preferably 0.3 to 1g based on 100 ml have.

Health functional food of the present invention includes the form of tablets, capsules, pills, liquids and the like.

The health functional beverage composition of the present invention is not particularly limited to other ingredients except for containing the compound as an essential ingredient in the indicated ratios, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks.

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally from about 1 g to 20 g, preferably from about 5 g to 12 g per 100 ml of the extract of the present invention.

In addition to the above, the extract of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the extract of the present invention may contain fruit flesh for the production of natural fruit juices and fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of the compound of the present invention.

The cosmetic composition may be provided for the use of basic cosmetics, makeup cosmetics, body cosmetics, hair cosmetics, scalp cosmetics, shaving cosmetics or oral cosmetics. Examples of the basic cosmetics include creams, lotions, packs, massage creams, emulsions, etc. The makeup cosmetics include foundation, makeup base, lipstick, eyeshadow, eyeliner, mascara, eyebrow pencil, and the like. Examples include soaps, liquid cleansers, baths, sunscreen creams and sun oils. Hair care products include shampoos, rinses, hair treatments, hair mousses, hair liquids, pomades, hair colorants, hair bleaches, and colorants. There are rinses, hair tonics and scalp treatments as scalp cosmetics, aftershave and shaving creams as shaving cosmetics, and toothpaste and mouth washes as oral cosmetics.

The present invention provides a composition for preventing and treating acne comprising a germinated bean extract as an active ingredient. The composition for preventing and treating acne comprising the germinated soybean extract is preferably an excellent effect on sebum secretion control effect, hyperkeratosis inhibitory effect, vesicle formation inhibition and alleviation effect, acne prevention and treatment, in addition to the germinated bean extract It may include a magnolia bark extract mainly composed of magnolol and honokiol.

People with acne-prone skin are usually susceptible to acne bacteria even if they keep their skin clean, which makes infections easier and leads to inflammation over time, resulting in aesthetic appearance. Therefore, the present invention has a strong antibacterial effect against germinated soybean extract and acne bacteria having anti-inflammatory effect and antioxidant effect, and excellent antibacterial effect even when applying a very small amount, safe for acne-sensitive sensitive skin (magnolol) or honokiol As a result of experiments using magnolia bark extracts containing (honokiol) as a main component, the synergistic effect of the two components was found to be very effective in treating and preventing acne skin.

The composition for treating or preventing acne of the present invention may include a germinated soybean extract, and preferably may include a germinated soybean extract and magnolia bark extract mainly composed of magnolol (magnolol) and honokiol. Germinated soybean extract of the present invention has an excellent anti-acne bactericidal activity with anti-inflammatory and antioxidant effects on acne skin.

In the composition for treating or preventing acne of the present invention, the content of the germinated bean extract is 0.001% to 50.0% by weight based on the total composition.

In addition, the present invention includes a germinated soybean extract and a magnolia bark extract, wherein the content of the germinated soybean extract is 0.001% to 50.0% by weight, respectively, the content of magnolia bark extract is 0.001% to 20.0% by weight, respectively And it provides a therapeutic composition. In another embodiment of the present invention, the composition for treating or preventing acne of the present invention is 0.001% to 50.0% by weight of germinated bean extract, 0.01% to 5.0% by weight of magnolol and hono based on the total composition It may contain 0.001% to 20.0% by weight of the magnolia bark extract, the main component of the honokiol. The blending ratio of the soybean germinated bean extract and the magnolia bark extract may be 10: 1 to 1:10, preferably 7: 3 to 3: 7, more preferably 3: 1 to 1: 1 by weight. 3, most preferably 1: 1.

Acne treatment and prevention composition according to the present invention was found to be more excellent antibacterial activity to acne bacteria at 0.1% by weight when mixed with germinated soybean extract 0.75% by weight of magnolia bark extract alone. In addition, the application of up to 20 times the antimicrobial activity MIC (minimum blocking concentration) showed little effect on the skin and excellent effect. Although it can be applied to the product even at 20 times the concentration of the antimicrobial activity MIC, the skin irritation is concerned, antimicrobial effect against acne bacteria does not increase proportionately or the rate of antibacterial activity may not be economically effective in terms of prescription efficiency. That is, it was confirmed that the effect of applying the magnolia bark extract 0.05 wt% to 10.0 wt% based on the total composition is excellent and economical, and can be applied to the maximum magnolia bark extract 20.0 wt%. In addition, when applying the anti-inflammatory germinated soybean extract 0.001% to 50.0% by weight, preferably 0.001% to 20.0% by weight, which is the same content as the magnolia bark extract, it is excellent in the treatment and prevention of acne skin It was confirmed to exhibit an effect.

In specific embodiments of the present invention, the present invention may be used in the form of a pharmaceutical composition, a food composition, or a cosmetic composition.

Carriers or excipients that may be additionally included in the composition for preventing and treating acne of the present invention may use a conventional material depending on the formulation, water, dextrin, calcium carbonate, lactose, propylene glycol, liquid paraffin, saline , Dextrose, sucrose, sorbitol, mannitol, ziitol, erythritol, maltitol, starch, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, polyvinylpyrrolidone, methylhydroxybenzoate, propylhydroxybenzoate, Talc, magnesium stearate and mineral oil, but are not limited thereto. Two or more carriers or excipients may be used. In addition, when formulating a composition for preventing and treating acne, it may further include conventional fillers, extenders, binders, disintegrants, surfactants, anti-coagulants, lubricants, wetting agents, flavors, emulsifiers or preservatives.

The composition for preventing and treating acne of the present invention may be prepared in a dosage form commonly used according to the type of pharmaceutical composition, food composition, or cosmetic composition, and when used as a medicament, may be prepared in a dosage form for oral or parenteral use. Can be.

Formulations of the composition may be selected and used in a preferred form depending on the method of use, and in particular may be employed by formulating methods known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal. Can be. Examples of specific formulations include PLASTERS, GRANULES, LOTIONS, LINMENTS, LIMONADES, POWDERS, SYRUPS, LIQUIDS AND SOLUTIONS, Aerosols ( AEROSOLS), Ointments, Ointments, EXTRACTS, Elixir, FLUID ExTRACTS, Emulsions, SUPESIONS, Premise, INFUSIONS, Eye drops, Tablets TABLETS), suppositories, INJECTIONS, spirits, capsules (CAPSULES), creams, soft or hard gelatin capsules or pills (PILLS) and the like.

The composition for preventing and treating acne according to the present invention may be used orally or parenterally, for example, it may be used through intradermal, intramuscular, intraperitoneal, intravenous, subcutaneous, nasal, epidural and oral routes, and administration thereof. The amount may be determined in consideration of the method of administration, the age, sex and weight of the recipient, and the severity of the disease.

In general, about 100 to 200 times the concentration used in the cell experiment is used in animal experiments, the dosage of the composition of the present invention, or the composition for immuno-enhancing, the absorption of the active ingredient in the age, sex, condition, body of the patient And it is good to determine in consideration of the drug used in combination, a day from 0.0001 mg / kg (body weight) to 100 mg / kg (based on the total content of the active ingredient, that is, germinated soybean extract, or germinated soybean extract and magnolia bark extract) Body weight), preferably 0.01 mg / kg body weight to 10 mg / kg body weight per day. However, the above dosage is only one example to illustrate and is not limited to the above range.

The composition for preventing and treating acne of the present invention may be used as a food composition, and the food composition may include an active ingredient, namely, a germinated soybean extract, or a germinated soybean extract and a magnolia bark extract and a food acceptable food supplement additive. Can be used as a nutraceutical.

Health functional foods to which the extract of the present invention may be added for the purpose of preventing and improving acne include various foods, beverages, gum, tea, vitamin complexes, and health functional foods. The amount of the extract added to the food or beverage may be added in an amount of 0.01% to 15% by weight of the total food weight, and the health beverage composition may contain 0.02 g to 5 g, preferably 0.3 g to 1 g based on 100 ml. Can be added in proportion.

Health functional food of the present invention includes the form of tablets, capsules, pills, liquids and the like.

The health functional beverage composition of the present invention is not particularly limited to other ingredients except for containing the compound as an essential ingredient in the indicated ratios, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks.

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally from about 1 g to 20 g, preferably from about 5 g to 12 g per 100 ml of the extract of the present invention.

In addition to the above, the extract of the present invention is a variety of nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, such as flavoring agents, coloring and neutralizing agents (such as cheese, chocolate), pectic acid and salts thereof, alginic acid and Salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. In addition, the extract of the present invention may contain fruit flesh for the production of natural fruit juices and fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of the compound of the present invention.

The composition for preventing and treating acne of the present invention may be used as a cosmetic composition, and the cosmetic composition may be applied as an active ingredient, that is, a skin external preparation including a germinated bean extract, or a germinated bean extract and a magnolia bark extract.

The cosmetic composition for preventing and treating acne is applied as an external preparation for skin, and specifically, a skin cleansing composition including a systemic cleanser and soap, a base cosmetic, a makeup cosmetic, a body cosmetic including a body cream / lotion, hair, etc. It may be provided for use in cosmetics, scalp cosmetics, shaving cosmetics or oral cosmetics. In addition, the cosmetic composition may be prepared in various formulations according to the use purpose.

Examples of the basic cosmetics include creams, lotions, packs, massage creams, emulsions, etc. The makeup cosmetics include foundation, makeup base, lipstick, eyeshadow, eyeliner, mascara, eyebrow pencil, and the like. Examples include soaps, liquid cleansers, baths, sunscreen creams and sun oils. Hair care products include shampoos, rinses, hair treatments, hair mousses, hair liquids, pomades, hair colorants, hair bleaches, and colorants. There are rinses, hair tonics and scalp treatments as scalp cosmetics, aftershave and shaving creams as shaving cosmetics, and toothpaste and mouth washes as oral cosmetics.

The manufacturing method of the cosmetic composition of this invention can be manufactured by the conventional method including the said effective ingredient based on the base of each general product. In addition, the composition of the present invention can be used in addition to the above-mentioned active ingredient and the basic base of each product, components contained in conventional skin external preparation components such as perfumes, pigments, fungicides, antioxidants, preservatives, moisturizers, thickeners, Inorganic salts, synthetic polymer materials and the like can be further used to improve physical properties.

The present invention relates to an antibacterial effect, in particular, anti acne bacterium effect and excellent antimicrobial composition, anti-inflammatory composition and acne prevention and treatment composition excellent in the prevention and treatment of acne, germinated soybean extract and acne bacteria having an anti-inflammatory effect and antioxidant effect It is very effective in treating and preventing acne skin by synergistic effect of the two extracts by using a combination of magnolia bark extract which is excellent in antibacterial effect and safe for acne sensitive skin.

Hereinafter, the present invention will be described in more detail with reference to preferred embodiments, but the present invention is not limited thereto.

Example  One: Germinated beans  Preparation of Extract

After adding 10 times purified water based on the mass of commercially available black soybeans and soaking for 36 hours at 25 ° C., the soaked beans were taken out and germinated at 27 ° C. for 84 hours to prepare germinated beans.

After adding 10 times 1,3-butylene glycol of the germinated soybeans to all the germinated soybeans so as to submerge all the germinated soybeans, germinated soybean extract was performed by cold extraction at 15 ° C. to 20 ° C. for 3 days. Was prepared. The obtained germinated bean extract was filtered using a filter and lyophilized to obtain a germinated bean extract.

Example  2: Germinated beans  Of extract PGE ₂ Production inhibitory effect (anti-inflammatory effect)

In order to investigate the effect on the COX-2 activity of germinated soybean extract, the following experiment was conducted [Ref: Jeffrey, KH, Anglea, SW, Zoe, S., and Rhia CM Intracellular Measurement of Prostaglandin E2: Effect of Anti- inflammatory Drugs on Cyclooxygenase Activity and Prostanoid Expression, Analytical Biochemistry, 1999, 271, 18-28].

Fibroblasts obtained by culturing primary cells isolated from human epidermal tissue were passaged several times, and then 1 × 10 ⁵ were placed in 96-well plates and incubated for 24 hours. The germinated soybean extract solution prepared by adding to the medium containing no FBS and incubating for 2 hours, and adding the germinated soybean extract DMEM medium obtained in Example 1 at a concentration of 10 ppm, 100 ppm and 1,000 ppm to The concentration was treated as shown in Table 1. In order to examine the effect on the COX-2 activity of the germinated bean extract, indomethacin (indomethacin) was added instead of the germinated bean extract in the comparative example.

After treating the germinated soybean extract or indomethacin, the mixture was incubated for 1 hour and treated with calcium ionophore A23187 and arachidonic acid at a concentration of 50 M for 5 minutes at 37 ° C.

Dodecyltrimethylammonium bromide was treated with a final concentration of 0.25% for 10 minutes to hemolyse the cells, and an appropriate amount of the supernatant was taken to quantify the amount of PGE ₂ using Enzyme-Linked Immunosorbent Assay (ELISA). By judging the prostaglandin inhibitory effect of the germinated soybean extract. The results are shown in Table 1 below.

sample Concentration (ppm) PGE ₂ (pg / 105 cells) Inhibition Rate (%) Negative control - 24.6 ± 4.5 - Positive control group (+ arachidonic acid) - 384.2 ± 15.1 - Germinated Bean Extract 10 327.3 ± 12.2 15.8 100  256.5 ± 10.6 35.5 1000 197.1 ± 11.4 52.0 Comparative Example (Indometacin Treatment Group) 3.5 59.3 ± 8.9 90.4

The inhibition rate is PGE ₂ of skin fibroblasts not treated with arachidonic acid and fibroblasts treated with arachidonic acid. The difference was calculated based on the difference in production amount, and in order to reduce the error range and ensure objectivity, the same test was repeated three times (n = 3).

It can be seen from Table 1 that the germinated soybean extract effectively inhibits the production of prostaglandins by 52% at the concentration of 1000 ppm. In particular, since the produced PGE ₂ is known to be mainly produced by the COX-2 enzyme, it can be seen from the above test that the germinated bean extract inhibits the inflammatory response by inhibiting the inducing action or activity of the COX-2 enzyme.

Example  2 : Germinated beans  Anti-inflammatory effect test of extract

To determine the anti-inflammatory efficacy of germinated soybean extracts in animal experiments, mouse ear swelling test (MEST) [A. Crummey, G. P. Harper, E. A. Boyle and F. R. Mangan. Inhibition of arachidonic acid-induced ear edema as a model for assessing topical anti-inflammatory compound. Agents and Actions 1987, 20, 69-76.

Before the sample application, 35 hairless mice were divided into 7 groups each, and both ears were thoroughly washed with ethanol and measured using a micrometer, and then the hairless mice were shown in Table 2 below. Germinated bean extract obtained by lyophilization in 1 was added to PBS buffer solution Example treated with 0.5% solution and 1.0% solution, Comparative group treated with indomethacin (indomethacin 1.0% solution, The experiment was performed by dividing into a control group treated with only arachidonic acid, and the average value of the ear thickness of the hairless mice was measured.

More specifically, 20 μl of the sample and 20 μl of ethanol for the control group were continuously treated once daily for 4 days for the sample group treated with the germinated bean extract and the indomethacin in both ears of the hairless mouse. After 1 hour of application, ethanol was applied to the left and arachidonic acid was applied to the right ear, and 2 mg / ear was applied to the right ear. After 1 hour of application, the edema of the ear was applied to both ears using a micrometer. The anti-inflammatory effect of germinated soybean extract was measured by repeated measurements three times. In addition, the left ear of the treatment group was used as a control to determine the degree of inflammation caused by each sample itself.

Table 2 shows the anti-inflammatory effect of determining the degree of edema inhibition based on the control group treated with arachidonic acid only from the measured results.

sample density(%) Ear thickness (㎛) % Inhibition Before sample processing After sample processing Sample group Germinated Bean Extract 0.5 325 548 20.4 Germinated Bean Extract 1.0 328 539 24.6 Comparison Indomethacin 1.0 324 468 48.6 Control Arachidonic acid 2mg / ear 321 601 -

The inhibition rate was calculated by the following Equation 1 using the average thickness change (A) of the control ear and the average thickness change (B) of the sample application group, in order to ensure the objectivity by reducing the error range, the same test was repeated three times the average value Comparison was made (n = 3).

[Calculation 1]

% Inhibition = (A-B) / A X 100

Mean thickness change of control ears (A) = thickness of arachidonic acid treated ear-thickness of untreated ear

Average thickness change (B) of sample application group ears = thickness of sample treated ears-thickness of untreated ears

As shown in Table 2, the germinated bean extract sample showed an inhibition rate of inflammation of 24.6%. These figures were about 51% of the anti-inflammatory effect compared to Indomethacin, which is an excellent anti-inflammatory effect as a pharmaceutical ingredient. In addition, when verifying the difference in edema increase rate, germinated soybean extract showed an effect of 99% significant difference with arachidonic acid.

Example  3: Germinated beans  Antioxidant Effect Test of Extracts

In order to confirm the antioxidant effect of the germinated soybean extract, experiments were carried out by measuring the radical scavenging effect using the antioxidant BHT and medicinal extract, and DPPH (1,1-Diphenyl-2-picryl-hydrazyl). .

Specifically, 4 mg of DPPH was dissolved in 100 ml of methanol to make a DPPH solution. In addition, the sample solution was prepared by diluting Eochochocho, Baenggulchae and vanilla extract in ethanol. More specifically, the Echochocho, Baenggulchae and vanilla extracts were added to 10 times 1,3-butylene glycol of each extraction target plant to lock all the extraction target plants, followed by cold extraction at room temperature for 3 days. The extract was prepared by diluting with ethanol to prepare a sample solution.

Germinated soybean extract solution, 1 ml of the prepared sample solution and 1 ml of DPPH solution were mixed and left at 37 ° C. for 30 minutes, and then absorbance was measured at 516 nm. BHT (butylated hydroxyl toluene) was used as a standard and ethanol was used as a control. The concentration of the active ingredient of the added solution is as shown in Table 3 below, from the results measured by the above method using the following formula 2 to measure the antioxidant power, the measured results are shown in Table 3 below.

[Calculation 2]

Antioxidant power (%) = 100-(absorbance of sample x 100) / (absorbance of control)

BHT Eochocho extract White bran extract Germinated Bean Extract 0.01% 90.2 69.4 49.7 82.9 0.001% 31.3 14.1 13.1 16.0

Referring to Table 3 above, as a result of comparing the antioxidant power through the radical scavenging ability by DPPH method, the antioxidant power of the germinated soybean extract was slightly lower than that of BHT, but was superior to the white bran extract, Echochocho extract. At 0.01% concentration, the antioxidant effect of germinated soybean extract was over 80%, indicating that it had sufficient antioxidant power.

Example  4: Germinated beans  And Magnolia bark  Antimicrobial Activity Evaluation Experiment of Extract

Magnolia bark extract used in this experiment was extracted by cold immersion, that is, immersed magnolia bark in 1,3-butylene glycol for 48 hours, and extracted through the cold immersion method and filtered through a filter. The content of magnolol or honokiol as an active ingredient included in the magnolia bark extract is 1.0%.

In order to determine the antimicrobial activity (bacterial ability) against pathogenic bacteria and especially acne bacteria (Propionibacterium acnes (P. acnes)) of magnolia bark extract containing germinated soybean extract, magnolol or honokiol Refrigerated S. aureus, E. coil, P. aeruginosa, C. albicans, A. niger, P. acnes (Korea) BHI solids containing a certain concentration of germinated soybean extract and magnolia bark extract were incubated in BHI (Brain Heart Infusion) solid medium 3 days before the start of the experiment. After the inoculation of the above-cultured bacteria in the medium and more than 3 days, the growth of the bacteria was observed to determine the MIC (minimum blocking concentration). The experimental results are shown in Table 4 as antimicrobial MIC results (unit:%) of the bark and germinated soybean extract.

Germinated Bean Extract Magnolia Bark Extract Germinated Bean Extract: Magnolia Bark Extract 7: 3 1: 1 3: 7 Pseudomonas aeruginosa (ATCC 6538) 1.20 0.120 0.41 0.35 0.25 Escherichia coli (ATCC 8739) 1.30 0.135 0.48 0.42 0.28 Pseudomonas aeruginosa (ATCC 15522) 1.20 0.113 0.46 0.31 0.26 Candida (ATCC 10231) 2.60 0.35 0.64 0.51 0.47 Soil Mold (ATCC 9642) 3.10 0.43 0.65 0.53 0.48 Acne Bacteria ( ATCC  6919) 4.30 0.75 0.55 0.10 0.35

As the results of the above experiments, the magnolia bark extract itself was superior to the acne bacteria, but when combined with the magnolia bark extract and germinated soybean extract alone, the antibacterial activity against not only pathogenic bacteria but also acne bacteria in particular. You can see this power. In addition, when the ratio of the germinated soybean extract and magnolia bark extract is 1: 1, it can be seen that the most synergistic effect is excellent.

Example  5 -6 and Comparative example  One

As such, various compositions were prepared using the germinated bean extract and the magnolia bark extract having excellent effects on the treatment and prevention of acne skin.

division Raw material name Content (weight) Control Example 5 Example 6 Comparative Example 1 Awards Purified Water Glycerine Propylene Glycol Butylene Glycol Xanthan Gum Total 100 2.0 3.0 5.0 0.1 Total 100 2.0 3.0 5.0 0.1 Total 100 2.0 3.0 5.0 0.1 Total 100 2.0 3.0 5.0 0.1 Yu Sang Hexyllaurate Cyclomethicone Glyceryl Stearate / Fiji 100 Stearate Fiji 40 Stearate 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Efficacy Ingredient Germinated Bean Extract Magnolia Bark Extract -- 5.0- 2.5 2.5 -5.0 additive Incense preservative Proper quantity Proper quantity Proper quantity Proper quantity

In the production methods of Comparative Examples 1 and 5 to 6, the oily ingredients were mixed at the weight ratios shown in Table 5, and heated and stirred at 70 ° C. to make them uniform. In a separate mixer, the water phase components are mixed in the weight ratios shown in Table 2, and 70 ° C. is added to the oil phase components with 70 ° C. warm stirring, and emulsified with a homomixer 4000 RPM for 4 minutes. After cooling, the potent ingredient and the additive were added at about 50 ° C, and again emulsified at 4000RPM for 4 minutes using a homomixer, followed by cooling at 28 ° C.

Example  7: skin primary irritation test

In order to check the skin irritation of the control group, Comparative Example 1, and Examples 5 to 6, 50 healthy adults and men and women were placed on the back region of a predetermined amount (0.2 g) for 24 hours, and then the pin chamber was After 4 hours of removal, the skin changes were visually read and the results are shown in Table 6.

sample Number of subjects Judgment result Stimulus + + +/- － Control 50 - 3 6 41 0.24 Example 5 50 - - One 49 0.02 Example 6 50 - - 2 48 0.04 Comparative Example 1 50 - 2 7 41 0.22

Criteria for the results described in Table 6 above are as follows.

-: No erythema or unusual phenomenon

+/-: slightly reddish

+: Significantly redder than the surroundings

++: reddening and swelling worse than the surroundings

Stimulus = [{(±) Number X 1} + {(+) Number X 2} + {(++) Number X 3}] / Number of Subjects

As shown in Table 6, when the germinated soybean extract having an anti-inflammatory and antioxidant effect was found to be lower than that of the general cosmetic compositions as a control, the irritation was lowest, and the antibacterial to acne bacteria Stimulation of the magnolia bark extract was similar to that of the control. As a result, it was confirmed that the germinated bean extract or the mixture of the germinated bean extract and the magnolia bark extract was safe for the skin.

Example  8: Evaluate Acne Relief

In order to see the acne alleviating effect of the control group, Comparative Examples 1 and 5 and Example 6, a blind test was performed on 60 healthy males and females between 20 and 30 years old with acne. .

The acne mitigation effect evaluation was randomly selected by 20 people divided into three groups, for each group was applied to the control group and Example 5 contained in the same container 2 times twice a day for 2 weeks, each group In this case, the control group and Comparative Example 1 were applied twice a day for 2 weeks, and the control group contained in the same container and Example 6 were applied twice a day for 2 weeks.

Evaluation of acne relief effect was carried out by combining a questionnaire and a naked eye survey on whether the acne symptoms worsened and alleviated through an interview after the application for 2 weeks, and the results are shown in Table 7 below.

As shown in Table 7, the acne relieving effect is very excellent when the group 1 contains only the germinated soybean extract and the group 2 contains only the magnolia bark extract and the group 3 contains only the magnolia bark extract and the germinated soybean extract. It can be seen.

As a result of the experiments of the present inventors, it was found that it is effective to simultaneously apply antimicrobial raw materials resistant to acne bacteria as well as anti-inflammatory and antioxidant raw materials to acne treatment and prevention products. The reason is that acne-prone skin is mostly susceptible to acne bacteria and is easily infected with most inflammation. In addition, as a result of experimenting with the raw material having an anti-inflammatory effect, it was confirmed that the germinated soybean extract effectively worked, and its effective content was 0.001 to 20.0 wt%.

Hereinafter, an example of the preparation of a pharmaceutical composition containing the compound of the present invention will be described, but the present invention is not intended to be limited thereto, but is intended to be described in detail.

Formulation Example 1. Powder  Produce

Buddlezasaponin IV 20 mg

Lactose 100 mg

Talc 10 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 2 Preparation of Tablet

Buddlezasaponin IV 10 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, tablets are prepared by tableting according to a conventional method for preparing tablets.

Formulation Example 3 Preparation of Capsule

Buddlezasaponin IV 10 mg

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium Stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

Formulation example  4. Manufacture of health drinks

Buddlezasaponin IV 100 mg

15 g of vitamin C

100 g of vitamin E (powder)

Iron lactate 19.75 g

3.5 g of zinc oxide

Nicotinamide 3.5 g

0.2 g of vitamin A

0.25 g of vitamin B1

0.3 g of vitamin B2

Water quantification

After mixing the above components according to a conventional healthy beverage production method, and then stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 l container, sealed sterilized and then refrigerated and stored in the present invention For the preparation of healthy beverage compositions.

Although the composition ratio is mixed with a component suitable for a favorite beverage in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

Claims (17)

Antimicrobial composition comprising germinated bean extract as an active ingredient. The antimicrobial composition of claim 1, wherein the extract is prepared by using water, alcohol having 1 to 5 carbon atoms, propylene glycol, 1,3-butylene glycol, or a mixed solvent thereof. The method of claim 1, wherein the antimicrobial composition is E. coli, Salmonella typhimurium, Staphyllococcus aureus , Candida albican ), Earth fungus ( Aspergillus) niger ) or Propionibacterium acnes ) having an antimicrobial activity against at least one selected from the group consisting of. A medicament comprising the composition according to any one of claims 1 to 3. A health functional food comprising the composition according to any one of claims 1 to 3. Cosmetic composition comprising a composition according to any one of claims 1 to 3. delete delete delete Pharmaceutical composition for preventing and treating acne comprising a germinated soybean extract and magnolia bark extract as an active ingredient. The method of claim 10, wherein the germinated soybean extract is prepared using water, alcohol having 1 to 5 carbon atoms, propylene glycol, 1,3-butylene glycol or a mixed solvent thereof as an extraction solvent Pharmaceutical composition. delete The method of claim 10, wherein the magnolia bark extract is prepared using water, alcohol having 1 to 5 carbon atoms, propylene glycol, 1,3-butylene glycol, or a mixed solvent thereof as an extraction solvent. Pharmaceutical composition. The pharmaceutical composition of claim 10, wherein the composition comprises 0.001% to 50.0% by weight of the germinated bean extract and 0.001% to 20.0% by weight of the magnolia bark extract based on the total composition. The pharmaceutical composition for preventing and treating acne according to claim 10, wherein the mixing ratio of the germinated bean extract and the magnolia bark extract is 7: 3 to 3: 7 by weight. Acne improvement cosmetic composition comprising a germinated soybean extract and magnolia bark extract as an active ingredient. A dietary supplement for acne improvement comprising germinated bean extract and magnolia bark extract as active ingredients.