KR101996732B1 - Cosmetic composition comprising concentrate of Omegiju fermented using magma seawater for improving wrinkles, elasticity or moisturizing the skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving wrinkles, improving elasticity or moisturizing skin comprising an omega-based agricultural product fermented with lava waters and containing the active ingredient as an active ingredient, wherein the ome- It is manufactured by host fermentation method. The Omega concentrate inhibits the activity of elastase, collagenase and MMP-1, and increases collagen type 1 gene expression to increase collagen biosynthesis. It was also confirmed that the concentrate had an excellent moisturizing effect by increasing the expression of AQP-3 and filaggrin.

Description

Technical Field [0001] The present invention relates to a cosmetic composition for improving wrinkles, improving elasticity, or moisturizing skin including omega-concentrate fermented by using lava seawater, and more particularly, to a cosmetic composition for concentrating omega-fermented using magma seawater for improving wrinkles,

The present invention relates to a cosmetic composition for improving wrinkles, improving elasticity or moisturizing the skin, which comprises a concentrated omega-concentrate fermented by using lava seawater as an active ingredient.

Skin is the body organs most closely contacted with the external environment in the human body and has the function of protecting the inside of the human body and can be roughly divided into epidermis, dermis and hypodermis. The skin is a complex organ composed of cells of very diverse functions and materials with proper properties. The dermal layer is made up of elastic solid materials (fibers) and viscous liquid substances, which maintains the original skin elasticity . Thus, the skin exhibits a response due to a unique physical property to mechanical tension, which is called viscoelasticity. The reason why the skin has such a viscoelastic property is that collagen fibers and elastin fibers form a unique three-dimensional structure in the substrate of proteoglycans. Generally, as you get older, you may experience decreased skin function and atrophy due to ultraviolet rays, pollution, stress, etc., and the number of skin cells decreases or the skin thickness decreases. In this process, the elastic fibers of the skin are deformed, and the elasticity of the skin is reduced or the skin is stretched.

Collagen is a major component of the skin, of which type Ⅱ collagen accounts for 80% and type Ⅲ collagen accounts for 15%. When skin is exposed to ultraviolet rays for a long time, a large amount of active oxygen is produced in the skin, and thereby expression of matrix metalloproteinase (MMP-1), an enzyme that degrades collagen among matrix metalloproteinases (MMPs) The activity is promoted to reduce collagen synthesis, which in turn reduces skin elasticity and wrinkles. Therefore, it may be a good strategy to find a substance that inhibits the expression and activity of MMP-1 and promotes collagen synthesis in the development of new materials for prevention and treatment of skin aging (Journal of Biological Chemistry, 274, 21491-21494 , 1999; Journal of Investigative Dermatology, 107 (3), 404-11, 1996)

The human body is composed mostly of water, which plays an important role in the maintenance of life. The moisture content of the normal skin is 70% in the body and 20% in the stratum corneum, which supports the elasticity and flexibility of the skin. When the water content of the stratum corneum is 10% or less, it is known that the skin becomes dry and causes skin troubles. The moisture balance of the skin is controlled by the epidermis. The stratum corneum of the epidermis is an accumulation of dead cells, but plays a very important role in maintaining the moisture of the skin. The amount of moisture in the stratum corneum produced by regular keratinization is always 10-20%. This is a mixture of sweat and sebum, which protects the skin from the outside, preventing moisture from evaporating from the stratum corneum. Natural Moisturizing Factor (NMF) and dermal lipids capture and retain moisture. The sebaceous membrane, NMF, and dermis are acting to keep the skin hydrated. NMF is a hydrophilic hygroscopic substance that is produced during the keratinization process and plays an important role in skin moisturization. It is made of amino acids and holds moisture in the horny layer. 2-pyrrolidine-5-carboxylic acid (PCA) is also of particular importance in NMF. It forms a layer with a matrix filled between each cell and maintains the inner moisture (Skin Barrier Journal, 4, 71-80, 2002).

Aquaporin (AQP) is a membrane pore protein that acts as a water channel that selectively interrupts the passage of other ions or solutes. It is also called aquaglyceroporin by passing glycerol together with water. It is present in human keratinocyte to prevent the loss of water molecules and function as skin hydration, wound healing and homeostasis. Therefore, it is possible to confirm the moisturizing effect of the raw material by increasing the expression of AQP-3. It has also been reported that the amount of hyaluronic acid in human skin decreases with aging, which is considered to be one of the direct causes of decreased skin elasticity and moisture content due to aging. The synthesis of hyaluronic acid is proceeded by HAS (Hyaluronic Acid Synthase) and the moisturizing effect of the raw material can be confirmed by increasing the expression of HAS-2 (Journal of Drugs in Dematology, 6, s20-4, 2007; Experimental Dermatology, 18 (12), 1028-1035, 2009).

Filaggrin (FLG) is a precursor to the natural moisturizing factor (NMF), one of the main ingredients that maintain hydration in the skin. NMF is composed of amino acids and derivatives. It increases the elasticity of stratum corneum layer by ionic interaction between keratin. Previous studies have shown that when FLG, a precursor of NMF, plays an important role in moisturizing and elasticity of skin, mutation of skin causes skin diseases such as skin dryness, itching, and atopy. Thus, such an increase in FLG leads to an increase in the synthesis of NMF, a skin moisturizing factor, thus confirming its efficacy as a moisturizing indicator (Journal of Investigative Dermatology, 118, 678-685, 2002; Journal of Investigative Dermatology, 128, 2145 -2151, 2008).

There are Jeju Ome Technology (Ome Origin) and Moon Jeong-ju as the raw materials of the ripe rice. Ome technology is manufactured in Jeju Island with its own Takju and Yakju, and Takju is also known as a teddy bear. The manufacturing method of ome technology is as follows. First, wash your baby in water and immerse in water for 5 to 6 hours. Then, digest the powder with millet, millstone, chung whale, etc., and make the dough. After removing the dough by fist, It makes omegi rice cakes (hole rice cakes). Add the prepared omegi rice cakes (boiled rice cakes) into boiling water, boil them for about 30 minutes, cook them, add the cooked omegi rice cakes in a large bowl, cool them thoroughly with a bat and hands so that the shape of the rice cakes disappears. After boiling the yeast powder on the well-diced rice dough, boil it and pour the cooled water little by little, mix it well, fill it with about 80% of the drink, cover it and keep it warm. The fermented ome technique is divided into two layers, the clear wince part of the upper part is sake, and the lower part is supported by the sieve. This liquor is characterized by the distinctive aroma of ripe rice and a sour, yet mild taste. The well-made liquor has a rich flavor that sticks to the mouth while sticking to the mouth and a distinctive flavor that sticks to the mouth. Cheongju, which is made of yellow oil, is considered to be precious and used for feast, sacrifice, and gut. Meanwhile, tsukbaji (Donggangju, It is a drink, rich in nutrients, and can be eaten without a special drink. It was mixed with barley rice and stored in a scam bottle and used as a vinegar. In Jeju, there is the word "mooju", which means that it is derived from the meaning of "drinking alcoholic beverages" with a drink such as a teddy bear (Han, Yong Hee, Baeksan Publishing House, October 24, 1999).

On the other hand, there is a distillation shochu in Jeju. It is a dialect of Jeju province which refers to a ring which is used to distill soju. High-quality sake is produced by distilling ome technology to produce soju, and recently rice is used as a raw material.

Foxtail millet (Italian millet) is a long-cultivated plant grown in prefecture in all temperate regions of Asia. It is said to have been introduced in China prior to the transfer of rice. Its name is Setaria italica and its name is Seosuk. Among the grains, the seedling is the smallest and the storage stability is strong. There are various kinds, but there are 'Chijo' with persistence and 'mezzo' which does not. The nutritional composition of the broiler contains about 10% of protein and contains half of prolamin and glutelin. The prolamin contains less lysine, leucine, There are many. The sugar content is about 70%, most of which is starch. The shape of this starch is similar to the starch of rice. Other ingredients include moisture, lipids, fibrin, and ash. It is mainly used to make rice, rice cake, confectionery, syrup and soju mixed with rice. In addition, there is a therapeutic effect of reducing the fever of the disease by making it into the dead, but the reason is not known (Korean Food Dictionary, edited by Park Won-gi, Shin Kwang Publishing Co., 1991.4.20).

In the prior art related to the manufacture of liquor using wheat flour, a distillation liquor consisting of rice straw or only a fermentation liquid of a fiber-containing substance such as grass or parsley has no flavor and is used as a beverage in order to solve problems of the prior art. (Korean Registered Patent No. 10-0001612), which comprises mixing rice and wheat bran by fermentation to prepare yeast, and making omegi rice cake with rice flour; Preparing yeast by mixing and fermenting seed yeast, lactic acid, omega rice cakes, and water with yeast; A step of immersing in osmagi rice cakes, entrance and fermentation for a predetermined period of time after addition of water; A two stage fermentation step of fermenting the fermented omegi rice cakes and koji added to the primary fermentation broth obtained in the fermentation stage 1 for a predetermined period of time after addition of water and koji; And a step of removing the seed from the secondary fermentation broth obtained in the second stage fermentation step, whereby a unique taste, aroma and color can be preserved for a long time. -2001-0017133). Korean Patent Laid-Open Publication No. 10-2014-0120402 discloses a process for producing omega pearls, wherein lava seawater having a salinity of 250 mg / l or less is used for producing omega pearls.

Lava seawater is a unique water resource possessed only by Jeju Island that has been aged for a long period of time as the seawater is naturally filtered through the basalt and siliceous layers and has been aged for a long time. Since the 1980s, the low temperature and cleanliness characteristics of lava seawater, Has been actively used as a breeding water for flounder farms and has been widely used since 5 to 6 years ago as a water for sauna and the like in terms of health and beauty. Lava seawater can be either used in desalinated lava waters that have been depleted of saline, or as collected, depending on the intended use.

Lava seawater contains more essential minerals (iron, manganese, zinc, molybdenum, etc.) as well as essential minerals such as sodium, magnesium, calcium and potassium than ordinary seawater, deep sea water and samdasu. Among them, vanadium which is known to stabilize insulin secretion, diabetes, hyperlipidemia, promotion of blood circulation, immunity enhancement, germanium having anticancer action, suppression of oxidation of fat, synergistic effect of maintaining heart and liver, The inclusion of selenium, which has the ability, anticancer, infertility, aging and cholesterol levels, is a feature of lava flows that have never been reported in deep ocean waters. In addition, these minerals are ionized, and ionized minerals are easily digested and absorbed by humans and other animals. In addition, lava seawater is a clean groundwater resource in which no E. coli, nitrate nitrogen, phosphorus phosphorus, phenol, etc. are detected, and harmful components such as arsenic, mercury and cadmium are not detected or lead is extremely small, There is no clean raw material.

Thus, the inventors of the present invention have been able to complete the present invention by confirming that the omega concentrate prepared by the traditional method of Jeju using such lava water has improved wrinkles, improved elasticity and moisturizing effect of the skin.

Korean Patent No. 10-1242442 (title of the invention: cosmetic composition containing fermentation crude extract, applicant: MacroCare Co., Ltd., registered on Mar. 06, 2013) Korean Patent No. 10-1347694 (Title of the Invention: Process for producing fermented product of desalted lava sea water and cosmetic composition using the fermented product obtained by the method and fermented product thereof) Applicant: Jeju Technopark, Foundation: Registration date: 2013 27) Korean Patent No. 10-0947637 (Name of the invention: Cosmetics containing desalted mineral water separated from lava sea water, Applicant: Biorand Co., Ltd., registered on Mar. 08, 2010) Korean Patent No. 10-1463012 (Name of the invention: composition for external application of skin using lava sea water minerals, Applicant: Jeju Techno Park Foundation, registered on November 12, 2014) Korean Patent No. 10-1402025 (Name of the invention: composition for external application for skin using desalted lava water) Applicant: Jeju Technopark, Foundation Date: May 26, 2014 Korean Patent Laid-Open No. 10-2014-0120402 (title of the invention: ome technology production method and ome technology manufactured therefrom, applicant: Seonhong Park, published on October 14, 2014) Korean Registered Patent No. 10-0001612 (Name of the invention: Method of Manufacturing Liquor, Applicant: Kim, Yi, Date of Registration: May 12, 1965) Korean Patent Laid-Open No. 10-2001-0017133 (the name of the invention: a manufacturing method of a liquor made from a wheat gluten meal, applicant: Huh Sung Rok, published on Mar. 05, 2001)

Lim Suk Won et al., 2 Understanding and Treatment of Skin Aging, Journal of Skin Barrier, 4, pp71-80, 2002. Han, Yong Hee Won, Baeksan Publishing House, October 24, 1999. Korean Food Dictionary, edited by Park Won-ki, Shin Kwang Publishing Co., 1991.4.20. Dumans M et al., Journal of Drugs in Dematology, 6, s20-4, 2007. Fraizer, S. et al., Journal of Investigative Dermatology, 107 (3), 404-11, 1996. Lee et al., J. Cosmetic Science, 49, 285-297, 1998. Nagase H and Woessner JF, Journal of Biological Chemistry, 274, 21491-21494, 1999. Sougrat R, et al., Journal of Investigative Dermatology, 118, 678-685, 2002. Hara-Chikuma M and Verkman AS. Journal of Investigative Dermatology, 128, 2145-2151, 2008. Tzellos TG et al., Experimental Dermatology, 18 (12), 1028-1035, 2009.

The present invention provides a cosmetic composition for improving the skin's wrinkles, improving elasticity or moisturizing, which comprises as an active ingredient an omega-concentrate fermented using lava seawater.

The present invention relates to a cosmetic composition for improving wrinkles, improving elasticity or moisturizing the skin, which comprises a concentrated omega-concentrate fermented by using lava seawater as an active ingredient.

The omega fermented by using the lava sea water was obtained by adding lava seaweed and leaven to a ripe rice cake and fermenting the omega concentrate. The concentrate of omega concentrate was obtained by removing alcohol from omega and concentrating it.

The omega fermented by using the lava water is produced by heating the rice gruel with steam to prepare and cooling the rice gruel; The following formula is prepared by adding lava seaweed and yeast to the rice cake and fermenting it; Adding a new lobster and lava seawater to the above-mentioned roots, fermenting the roots to produce an extract, and aging and filtering it.

More preferably, the step of heating the glutinous rice with steam at 90 to 120 ° C for 10 to 30 minutes to prepare and cool the soybean paste; In the formula, 100 parts by weight of high- And 20 to 40 parts by weight of yeast are added and fermented at 25 to 28 DEG C for 40 to 50 hours; 200 to 400 parts by weight of new capsicum and 100 to 160 parts by weight of fresh lava seawater were added to 100 parts by weight of the above foundation and fermented with stirring at 25 to 28 ° C for 40 to 50 hours to prepare an explant. Lt; 0 > C for 20 to 40 days, adding diatomaceous earth to the aged paste, and filtering with a filter.

The concentrate may be one in which the alcohol is removed from the omega extract and then concentrated.

The concentrate is effective to inhibit the activity of elastase or collagenase. The concentrate is also effective in increasing the expression of AQP-3 or Filaggrin.

The present invention can provide a cosmetic for improving the wrinkles of skin, improving elasticity or moisturizing the skin, which comprises a concentrate of omega-starch fermented by using the lava water. When the weight of the cosmetic is 100% by weight based on the total weight of the cosmetic, the cosmetic may contain 0.001 to 20% by weight of an omega extract.

The formulation of the cosmetic composition preferably comprises at least one of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizer cream, a hand cream, , Soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

In another aspect, the present invention provides a composition for improving wrinkles, improving elasticity, or moisturizing the skin comprising the omega concentrate fermented using the lava waters as an active ingredient.

Hereinafter, the present invention will be described in detail.

The lava water of the present invention is characterized in that the underground seawater is taken at a depth of 30 to 150 m, preferably 44.35 m, 86.35 m, or 126.35 m underground (based on an average sea level) by excavating 100 to 200 m underground in Kanglae- do.

The step of fermenting omega fermented with lava water used in the present invention comprises the steps of: heating glutinous rice with steam to prepare and cooling it; The following formula is prepared by adding lava seaweed and yeast to the rice cake and fermenting it; And adding a new lobster and lava seawater to the above-mentioned beads and fermenting the same to produce an extract, and aging and filtering the same.

The step of heating the millet with steam and preparing the millet is cooling the millet by heating the millet at 90 to 120 ° C for 10 to 30 minutes.

The liquor was prepared by adding lava seaweed and leaven to lobster and fermenting 100% And 20 to 40 parts by weight of yeast are added and fermented at 25 to 28 DEG C for 40 to 50 hours. If the fermentation is not carried out well, it is not preferable to use it as a raw material for the lyophilization.

In the step of aging and filtering, 200 to 400 parts by weight of high sugar meal and 100 to 160 parts by weight of lava seawater are added to 100 parts by weight of lecithin, , And fermenting at 25 to 28 DEG C for 40 to 50 hours with stirring. The aging step is carried out by maintaining the paste at 22 to 28 ° C for 20 to 40 days. The filtration step is performed by adding diatomaceous earth to the aged paste and filtering with a filter. It is not suitable to be used as cosmetics because it does not exhibit the effect of improving wrinkles because the fermentation is not sufficiently carried out if it is used as a base of ome.

The above process is more preferably as follows.

[The step of heating the millet to steam to make and cook it]

It is a step to build rice cake and cool down by adding steamed rice to millet. The steam temperature is 90 to 120 캜, preferably 100 to 120 캜. The steam heating time is 10 to 30 minutes, preferably 10 to 20 minutes. As the above-mentioned meats, rice mezzo or rice can be used. At a temperature and a time outside the above-mentioned range, the temperature and the time are preferable because no hot pepper suitable for omega production is produced.

[The formula is the step of making the molasses by adding the lava sea water and the yeast to the rice cake and fermenting it]

The process is to mix lava seaweed and fermentation source with high sugar content to make a lumber.

The fermentation source is a microorganism that causes a fermentation action, and the fermenting bacteria themselves such as fungus fungi, yeast, lactic acid bacteria and the like may be used, or dried yeast may be used by naturally propagating the fermenting bacteria in the grain. In this case, the use of the yeast is effective for the production of omega strains by activating the fermenting bacteria.

The yeast is a fermentation enzyme produced by pulverizing a grain, which is a mixture of rice, barley, corn, sorghum, barley, wheat, etc., alone or in combination, and drying fungi, yeast, lactic acid bacteria and the like in a natural state. Nuruk can be either commercially available or sold or can be manufactured directly. Specifically, 10 to 30 parts by weight of purified water is added to 100 parts by weight of the ground cereal, and the mixture is kneaded, wrapped in a cloth, straw, grass leaf, etc., Then, it can be manufactured by drying in a drier which is kept at a temperature of 40 to 60 ° C for 3 to 5 days after fermentation for 7 to 40 days by placing straw or mugwort on a thermostat maintained at a temperature of 30 to 40 ° C. In recent years, rather than natural fermentation, the fermentation broth is directly added to the fermentation broth to produce the fermentation broth, and the fermentation broth is smoothly distributed on the market. Such commercially available fermentation broth can also be used in the present invention.

When the fermenting bacteria are used as the fermenting bacteria themselves, it is preferable to add 60 to 100 parts by weight of lava seawater and 30 to 60 parts by weight, preferably 45 to 50 parts by weight, of fermentation source based on 100 parts by weight of roasted rice. If the amount of lava seawater is less than 60 parts by weight, 100 parts by weight of the lobster may not be well fermented. If the amount of the lava seawater exceeds 100 parts by weight, the fermentation may not be performed well. When the fermentation source is used as yeast, it is preferable to add 20 to 40 parts by weight, preferably 25 to 30 parts by weight, based on 100 parts by weight of roasted rice. If the amount is smaller than the above range, the fermentation is insufficient or the fermentation amount is excessive. The above-mentioned grafting may include fermentation at 25 to 28 ° C for 40 to 50 hours, preferably 47 to 49 hours. The temperature and the fermentation time are the most suitable temperature and fermentation time in the production of the present invention. If the temperature and the fermentation time are outside the above ranges, there is a problem that the preparation of the bees is insufficient or the taste of the final liquor deteriorates.

[A step of adding a new rice cake and lava seaweed and fermenting it to produce an extract, followed by aging and filtering]

The preparation of the lyophilized product is a step of adding lyophilized lava and lava water to the above-mentioned incision and making the lyophilization through fermentation. 200 to 400 parts by weight, preferably 300 to 400 parts by weight, of liquorice is added to 100 parts by weight of the foundation, and 100 to 160 parts by weight, preferably 120 to 150 parts by weight of lava water is added, Followed by fermentation at 28 ° C for 6 to 30 hours with stirring for 40 to 50 hours. Next, you can mature the eruption. Specifically, it is accomplished by aging at 22 to 28 ° C for 20 to 40 days, preferably 30 days. If it is out of the above range, aging can not be carried out, so that it may not be possible to produce omega seeds suitable for use as a raw material of cosmetic materials in the present invention. During the aging process, the aging process is carried out without further agitation.

Diatomaceous earth can be added to the aged paste and filtered using a filter. By carrying out this process, it is possible to manufacture omega fermented with lava sea water.

On the other hand, when the omega starch is produced, a process of removing fermentation odor and alcohol components is required for easy use as a raw material of cosmetic ingredients. Therefore, the omegine can be prepared from the omega concentrate used in the present invention by removing the alcohol through heating and concentrating it. The alcohol and fermentation odor generated in the fermentation are removed during the heating process for concentrating the omega host. If it is out of the above range, the fermentation odor is not removed or the alcohol remains, so that it can not be used as a cosmetic product. The concentration process is preferably carried out at 50 ° C to 70 ° C for 1 to 3 days, preferably for 2 days to remove the fermentation residue, and then the reduced pressure is concentrated at 50 to 80 ° C, preferably 55 to 65 ° C So that the alcohol produced during fermentation can be removed. The concentrate can be preserved or used even in a liquid state in a state where the alcohol is almost removed, but it is more preferable that the concentrate is prepared in a powder state in order to remove the fermentation process in the most desirable state. Before using it as a raw material for cosmetics, And the like.

The omega-concentrate of the present invention inhibits the activity of elastase, collagenase and MMP-1 and increases Collagen type 1 gene expression to increase collagen biosynthesis. It was also confirmed that the concentrate had an excellent moisturizing effect by increasing the expression of AQP-3 and filaggrin.

The present invention relates to a cosmetic composition for improving wrinkles, improving elasticity or moisturizing the skin, which comprises a concentrated omega-concentrate fermented by using lava seawater as an active ingredient.

The present invention can provide a cosmetic comprising the cosmetic composition containing the omega-concentrate fermented by using the lava water as a raw material. The cosmetic composition may contain 0.001 to 20% by weight of a cosmetic composition raw material containing omega concentrate fermented by using the lava sea water based on the total weight of the cosmetic composition as 100% by weight.

The formulations of the cosmetics are not particularly limited, but preferably include skin lotions, skin softeners, skin toners, astringents, lotions, milk lotions, moisturizing lotions, nutritional lotions, massage creams, nutritional creams, , Nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

The cosmetic of the present invention may further comprise a component selected from the group consisting of water-soluble vitamins, oiliness vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The water-soluble vitamin is not particularly limited as long as it can be compounded in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, And their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbate-2-phosphate etc.) can also be added to water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

As the vitality-imparting vitamins, vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) and the like can be exemplified, Alpha-tocopherol vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl laurate, and the derivatives thereof (palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbin, dl-alpha tocopherol acetate, Ether, etc.) are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in the cosmetic, and preferably collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymer polysaccharide may be any compound as long as it can be compounded in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

Seaweed extract can be any compound that can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of brown algae extract, red algae extract, green algae extract, and the like. Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic composition of the present invention may further contain other components to be blended with ordinary cosmetics.

Examples of the compounding ingredients that may be added include organic solvents such as a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, A blood circulation accelerator, a cold agent, an antiperspirant agent, and purified water.

Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.

Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Butyl isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri , Isostearyl stearate, isostearyl stearate, isodecyl stearate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isopropyl stearate, isopropyl stearate, isopropyl stearate, -Hexyl stearate, stearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propyleneglycol propionate, propyleneglycol propionate, dicaproic acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyldodecyl Octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, Stearoyl hydroxystearic acid isostearyl, 12-stearoyl stearyl hydroxystearate, 12-stearo And monohydroxystearic acid and esters such as sostearyl.

Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .

Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE (Polyoxyethylene / polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, polyether-modified silicone, polyoxyethylene-polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium chloride, Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p- (carbo-2'-ethylhexyl- , 3,5-triazine, 2- (2-hydroxy Methyl-5-methylphenyl) benzotriazole, and the like.

Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, any of the above components may be blended within the range not to impair the objects and effects of the present invention, but is preferably 0.01 to 5% by weight, more preferably 0.01 to 5% by weight, May be formulated in an amount of 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetics in addition to the above-mentioned extracts. For example, conventional auxiliary substances such as stabilizers, solubilizers, vitamins, .

When the cosmetic formulation of the present invention is a paste, cream or gel, animal fibers, plant fibers, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the cosmetic formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of spray, a mixture of chlorofluorohydrocarbons, Propane / butane or dimethyl ether.

When the cosmetic preparation of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the cosmetic preparation of the present invention is in the form of a suspension, it may contain a carrier liquid such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the cosmetic preparation of the present invention is a cleansing agent containing an interfacial active agent, it is preferable to use, as the carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.

In another aspect, the present invention provides a pharmaceutical composition for improving the skin's wrinkles, improving elasticity or moisturizing, which comprises as an active ingredient a concentrate of omega-starch fermented with lava seawater. The content of the extract contained in the pharmaceutical composition is not particularly limited. The pharmaceutical compositions may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterilized injection solutions according to conventional methods. Examples of carriers, excipients and diluents that can be contained in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , Methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient, such as starch, calcium carbonate, sucrose, Lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The dosage of the pharmaceutical composition of the present invention will depend on the age, sex, body weight of the subject to be treated, the particular disease or condition to be treated, the severity of the disease or condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of ordinary skill in the art and generally the dosage ranges from 0.01 mg / kg / day to approximately 2000 mg / kg / day. A more preferable dosage is 1 mg / kg / day to 500 mg / kg / day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, livestock, humans, and the like in various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine dural or intracerebral injection. Since the extract of the present invention has little toxicity and side effects, it can be safely used even for long-term administration for the purpose of prevention.

The present invention relates to a cosmetic composition for improving wrinkles, improving elasticity or moisturizing skin comprising an omega-based agricultural product fermented with lava waters and containing the active ingredient as an active ingredient, wherein the ome- It is manufactured by host fermentation method. The Omega concentrate inhibits the activity of elastase, collagenase and MMP-1, and increases collagen type 1 gene expression to increase collagen biosynthesis. It was also confirmed that the concentrate had an excellent moisturizing effect by increasing the expression of AQP-3 and filaggrin.

In recent years, a cosmetic composition having enhanced moisturizing power, elasticity enhancement, and anti-inflammation of skin containing desalted mineral water prepared by desalting lava seawater has been spotlighted as a raw material for beverage, liquor, medicine, (Korean Patent No. 10-1463012) for skin whitening activity, wrinkle-reducing activity and UV-blocking activity of lava sea water minerals (Korean Patent No. 10-1463012) As described in the present invention, there is no previous literature disclosing that omega seeds prepared by the traditional method of Jeju using lava untreated seawater have improved wrinkles, improved elasticity or moisturizing effect of the skin.

Fig. 1 shows the HPLC spectra of omega-concentrate fermented using lava water of Example 1 of the present invention.
Fig. 2 shows the HPLC spectra of the concentrated omega-concentrate fermented using the purified water of Comparative Example 1 of the present invention.
Figure 3 shows HPLC spectral results for tryptophan standards.
Fig. 4 shows the results of comparing the expression of Collagen type 1 gene in fibroblasts treated with omega-concentrate of Example 1 and Comparative Example 1. Fig.
Fig. 5 shows the results of comparing MMP-1 gene expression in fibroblasts treated with omega-concentrate of Example 1 and Comparative Example 1. Fig.
Fig. 6 shows the results of comparing mRNA gene expression to aquaporin-3 (AQP-3) in fibroblasts treated with omega-concentrate of Example 1 and Comparative Example 1. Fig.
FIG. 7 shows the results of the expression of the filaggrin (FLG) protein in the fibroblasts treated with the omega concentrate of Example 1 and Comparative Example 1 (upper-western blotting protein electrophoresis band and lower-band intensity quantification) .

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.

Production Example 1. Lava Seawater Intake>

Lava seawater brine was excavated using a sterilization filter at a depth of 150m underground (at an average sea level of 44.35m) at Handongri, Bukjeju-gun, eastern Jeju Island. The eradicated lava water (unsalted salt water) was used for the subsequent manufacture of omega seeds. The mineral composition of the eradicated lava sea water is shown in Table 1 below.

Minerals and heavy metals Concentration in lava sea water (ppm) Na 10190 Ma 1323 Ca 403 K 388 Mo 0.020 V 0.021 Ge 0.005 Br 71.2 Sr 10.2 B 0.58 Mn 0.002 Pb ND F ND As ND Cr ND CD ND Ar 0.0065

≪ Example 1: Preparation of concentrate of lava sea water ome &

Milk powder was steamed at 100 ° C for 20 minutes to prepare a rice cake. Nuruk was prepared by adding 20 g of purified water to 100 g of rice, kneading it, wrapping it in a cloth, putting it in a mold, putting it on the foot, covering the straw in a thermostat kept at 35 ° C, fermenting it for 20 days and drying it for 4 days in a dryer maintained at 50 ° C .

80 g of lava sea water and 30 g of Nuruk were added to 100 g of millet headed rice and fermented at 25 캜 for 48 hours to prepare a slab. For the preparation of the paste, 300 g of new capsicum and 130 g of fresh lava water were added on the basis of 100 g of the above-mentioned foundation, and the mixture was fermented at 25 캜 for 6 hours with stirring for 45 hours. The fermented paste was aged at 25 ℃ for 30 days. Diatomaceous earth was added to the aged paste and filtered through a filter to prepare omega seeds. The prepared omega host was heated at 60 DEG C for 2 days to remove alcohol and concentrated under reduced pressure at 60 DEG C to prepare omega concentrate in powder form. The powdered omega concentrate thus obtained is in a state suitable for use as a raw material for cosmetics by removing the fermentation odor.

≪ Comparative Example 1: Production of omega-concentrate fermented with purified water >

The omega concentrate powder was prepared in the same manner as in Example 1 except that lemon waters were prepared using purified water instead of lava waters.

≪ Comparative Example 2 > Preparation of desalted lava sea water powder &

Desalinated lava water was prepared by an electrodialysis method as disclosed in Korean Patent No. 10-0982351 (patent owner: SK Bioland), and the desalted lava water was concentrated under reduced pressure at 60 ° C to prepare a powder.

≪ Comparative Example 3 > Preparation of Concentrates of Fermented Milk of Ulmus japonica &

1kg of millet was crushed and powdered, and the same amount of purified water was added and it was called at room temperature for 6 hours. After sterilization at 121 캜 for 30 minutes, 50 g of yeast (the same as that used in Example 1) was added based on 100 g of sterilized millet and fermented for 48 hours. After fermentation, the mixture was filtered using 4 ㎛ paper filter paper, and the solids were removed to obtain a liquid fermented product. The filtrate was concentrated under reduced pressure at 60 ° C to prepare a powder.

≪ Comparative Example 4: Production of concentrate of fermented seaweed yeast &

50 g of sucrose and 550 g of MRS broth (DB Difco) are added to 10 kg of lava sea water to prepare an MRS liquid medium. The MRS liquid medium was supplemented with 10 g of koji (the same as that used in Example 1), pre-cultured, re-inoculated into the new culture, and cultured for 3 days. After culturing, the mixture was filtered using a 4 μm paper filter paper, and the solids were removed to obtain a liquid fermented product. The filtrate was concentrated under reduced pressure at 60 ° C. to prepare a powder.

≪ Comparative Example 5: Production of concentrate of fermented seaweed fermentation product of desalted lava sea &

The desalted lava sea water was prepared by the electrodialysis method disclosed in Korean Patent No. 10-0982351 (patent owner: SK Bioland), and the fermented product obtained by performing the fermentation process as in Comparative Example 3 with the desalted lava sea water Respectively.

≪ Comparative Example 6 > Preparation of fermentation broth powder prepared by general yeast fermentation method >

A fermentation broth was prepared in the same manner as in Example 1 except that white rice was used instead of millet, and a fermentation broth was prepared using purified water instead of lava sea water.

Experimental Example 1. Comparison of Fermentation Metabolites:

In order to confirm the difference between the omega concentrate fermented using lava seawater prepared in Example 1 and Comparative Example 1 and the omega concentrate fermented with purified water, the fermentation metabolites Respectively.

As a result, as shown in Fig. 1, it was confirmed that the omega concentrate fermented with lava seawater greatly increased in various components compared to the omega concentrate fermented with purified water of Fig. It was confirmed from the results of FIG. 3 that one of these is tryptophane, which is an essential amino acid component. As a result, it can be seen that the omega concentrate fermented using lava sea water containing a large amount of minerals has a significant difference in the oil-soluble components in the composition as compared with the omega concentrate fermented using purified water.

Column Shiseido CAPCELL PACK C18 Column, 5 탆 4.6 x 250 mm Mobile phase 20% methanol Detector UV 220nm Flow rate 1 ml / min Injection volume 20 쨉 l Column temperature
(Column Temprature) 35 ℃

Experimental Example 2: Elastase inhibitory effect confirmation [

For the compositions of Example 1 and Comparative Examples 1 to 6, the inhibitory effect of elastase activity was measured as described below (Lee et al., J. Cosmetic Science, 49, 285-297 , 1998).

(Elastase substrate Succ-Ala-Ala-Ala-p-nitroanilide standard product (SIGMA, S4760) was added to 60 mu l of the buffer solution (0.267 M Tris solution was adjusted to a pH of 8.0 with 0.267 M hydrochloric acid (Prepared by diluting each of the samples of Examples 1 to 4 with purified water to a predetermined concentration) were mixed and the resulting mixture was mixed with an enzyme solution (10 쨉 g / ml, pork pancreas elastase standard: Porcine Pancreatic Elastase, SIGMA, E-1250) was added, and the mixture was shaken and reacted at 25 ° C for 15 minutes. The amount of p-nitroaniline produced was measured at a wavelength of 410 nm. The absorbance C was measured using the solution obtained by manipulating the same method as that of the reference solution by adding 20 μl of the substrate solution, 20 μl of purified water and 100 μl of the sample to 60 μl of the buffer solution. The absorbance A was measured using a liquid prepared by diluting 100 μl of purified water instead of the sample with the same procedure as the sample solution. The absorbance A was measured, and 120 μl of purified water was added in place of the enzyme solution and the sample solution. , And the absorbance D thereof is measured, and the elastase inhibiting rate (%) is obtained according to the formula (1).

Equation 1

Elastase inhibition rate (%) = [1 - (enzyme activity of each extract concentration / enzyme activity of control group)] x 100

The ELASase inhibitory activity IC ₅₀ of Table 3 below represents the concentration (쨉 g / ml) of the substance required to inhibit the activity of the elastase by 50%. The results of the extracts of Example 1 and Comparative Examples 1 to 6 are shown in Table 3 below. Oleanolic acid was used as a control for comparison of activities.

sample IC ₅₀ ([mu] g / ml) Oleanolic acid 7.5 Example 1 10.2 Comparative Example 1 31.5 Comparative Example 2 115.1 Comparative Example 3 47.5 Comparative Example 4 57.9 Comparative Example 5 49.2 Comparative Example 6 72.5

From the results of Table 3, it can be seen that the omega concentrate fermented by using the lava seawater of Example 1 showed an excellent inhibitory effect on elastase compared with the other omega concentrate, and thus the effect of improving wrinkles was the best.

<Experimental Example 3> Measurement of moisture resistance>

Moisturizing effects of the compositions of Example 1 and Comparative Examples 1 to 6 were measured by using a corneometer CM 825 (Corneometer CM 825, Courate + Khazaka) as follows, and each distilled water was used as a control . The powdery compositions of Example 1 and Comparative Examples 1 to 6 used in the experiment were dissolved in purified water to 2.0% (w / w) at room temperature and then used in the following experiment.

Eighteen women in their twenties were divided into 2 x 2 ㎠, 8 compartments, washed several times with water, and water was removed without stimulation. After lapse of 15 minutes, 10 μl of each of the two fractions of the omega-fermented soybean extract of Example 1 and Comparative Example 1 and the extract solution of Comparative Examples 2 to 6 was uniformly applied. After 2 hours, measurements were made 5 times per compartment using a Corneometer CM 825 (Corneometer CM 825, Courate + Khazaka). Experiments were carried out in a constant temperature and humidity room with a room temperature of 21 ± 2 ° C and a relative humidity of 50 ± 5%. An arbitrary unit (AU) is displayed by pressing the probe of the corneometer in close contact with the surface of the measurement site and lightly pressing it. The measured values were averaged by the following formula (2), and the average values of the subjects were shown in Table 4.

Equation 2

Moisture (Corneometer touch, Corneometer Value) = △ S - S

S: Cordneome touch before sample application

△ S: 2 hours after sample application,

In order to more clearly demonstrate the efficacy against moisturizing, the following experiment carried out was conducted in the same manner as in Example 1 except that the omega concentrate (Example 1) fermented using lava seawater and the omega concentrate (Comparative Example 1) .

As a result of the experiment, it is confirmed that the composition of Example 1 has higher water holding capacity than that of the distilled water used as a control and the compositions of Comparative Examples 1 to 6, as shown in Table 4 below.

object Moisture power Control Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Subject 1 2.7 27.6 19.4 11.1 9.9 10.5 11.3 13.7 Subject 2 4.3 27.1 17.9 10.7 8.9 9.2 9.8 15.1 Target person 3 2.9 26.7 19.1 9.9 11.1 11.5 10.8 11.9 Subject 4 3.2 24.4 19.7 8.1 8.9 10.0 9.3 14.1 Subject 5 3.8 25.8 18.7 11.5 11.9 9.7 10.5 13.2 Subject 6 3.1 32.1 18.1 8.7 9.1 8.8 11.1 15.4 Target person 7 4.1 39.1 18.4 12.7 8.1 8.5 9.8 11.9 Target person 8 1.7 34.3 17.1 11.6 11.5 11.5 10.9 11.7

On the other hand, the subsequent experiments were conducted using the concentrate of the lava sea water omega concentrate of Example 1 and the concentrated concentrate of the omega concentrate of Comparative Example 1.

EXPERIMENTAL EXAMPLE 4. Confirmation of cytotoxicity test:

To measure the wrinkle effect and the moisturizing effect of the concentrate of the lava sea water omega main concentrate of Example 1 and the concentrate of the omega concentrate of the purified water of Comparative Example 1, toxicity tests (MTT assay) were performed in keratinocytes (HaCaT) and fibroblasts Respectively. MTT assay is an experimental method widely used for cell proliferation and toxicity by measuring the number of living cells. The living cells are soluble in mitochondria and have a yellow salt, MTT (3- [4,5-dimethlythiazole-2-yl] -diphenyl tetrazolium bromide) is reduced to a blue formazan derivative which is insoluble in water by succinate dehydrogenase or mitochondrial dehydrogenase. (100 U / ml), streptomycin (100 μg / ml) and DMEM containing 10% FBS (Fetal Bowen Serum) were inoculated on the bottom of the culture dish, (Using Dulbecco's modified Eagle's medium-HaCaT cell) and IMDM (using Iscove's modified dulbecco's medium-fibroblast cell) medium, and cultured in an incubator containing 5% carbon dioxide at 37 ° C. HaCaT cells and fibroblasts were diluted to a concentration of 1 × 10 ⁵ cells / ml on a 24-well plate and inoculated with 1 ml of each, and cultured for 24 hours. After the cultivation, the medium was removed, and the medium containing no serum was added thereto, and the extracts of Example 1 and Comparative Example 1 were treated for each concentration. After incubation for one day in an incubator containing 5% carbon dioxide at 37 ° C, all of the medium is removed, and the medium is diluted 10 times with 2.5 mg / ml MTT solubilization solution and cultured in a 4-hour incubator. After 4 hours, the supernatant was removed, and 1 ml of DMSO (dimethyyl sulfoxide) solution was added, and the resulting MTT formazan crystals were measured for absorption at 570 nm. The control used was DMSO (dimethyyl sulfoxide) used as a dissolution solvent. The toxicity test results are shown in Table 5 and Table 6.

Toxicity test results in fibroblast cell Sample Conc. (占 퐂 / ml) Cell viability (%) Control 100.00

Example 1 One 112.73 10 105.06 25 118.94 50 108.44 100 115.84 250 119.41 500 126.46

Comparative Example 1 One 94.69 10 96.88 25 96.18 50 97.63 100 99.18 250 105.50 500 113.16

Toxicity test results on keratinocyte HaCaT cells Sample Conc. (占 퐂 / ml) Cell viability (%) Control 100.00

Example 1 One 100.56 10 100.65 25 98.94 50 100.47 100 97.00 250 96.42 500 92.00

Comparative Example 1 One 96.87 10 96.80 25 96.13 50 94.32 100 91.10 250 90.34 500 89.98

The concentrate of the lava sea water omega-concentrate of Example 1 and the concentrate of the omega-concentrate of the purified water of Comparative Example 1 did not show any toxicity at a concentration of 500 μg / ml or less.

Experimental Example 5: Confirmation of collagen biosynthesis effect [

In order to measure the promoting effect of the collagen synthesis promoting effect of the concentrate of the lava sea water omega of Example 1 and the concentrate of the omega-concentrate of the purified water of Comparative Example 1, a commercially available human fibroblast was treated with a sample for Collagen type I gene expression experiment Respectively. The human normal fibroblast (CCD-985sk) purchased from the American Type Culture Collection (ATCC) together with IMDM (Iscove's Modified Dulbesco's Media) medium containing 10% fetal bovine serum was dispensed into a 60 mm dish at a concentration of 10,000 cells / well And cultured for 24 hours at 37 ° C and 10% CO ₂ . After 24 hours, the samples are diluted in IMDM medium containing no fetal bovine serum for each day, and the cells are collected with TRIzol (Invitrogen, USA) to isolate the RNA. The isolated RNA was quantitated using Qubit fluorimeter with RNA BR Assay kit, amplified using Real-Time PCR: 7500 Fast with Power SYBR Green PCR Master Mix (Applied Biosystems, USA) Respectively. The collagen type I and β-Actin primers used in the PCR were synthesized in Cosmos TECH (Korea). The primer sequences are shown in Table 7, and the experimental results are shown in Table 8 and FIG.

Type I collagen beta -actin Sense 5'-AGC AAG AAC CCC AAG GAC AA-3 ' 5'-GGC ACC CAG CAC AAT GAA G-3 ' Antisense 5'-CGA ACT GGA ATC CAT CGG TC-3 ' 5'-CCG ATC CAC ACG GAG TAC TTG-3 '

Sample Conc.
(占 퐂 / ml) Col-I Expression rate (%) Stdev. Control 100.00 0 Vitamin C 100 118.56 4.57 Example 1 500 153.47 7.48 Comparative Example 1 500 132.99 2.27

Referring to the results of Table 8 and FIG. 4, the concentrate of omega main fermented by using lava seawater was significantly larger than that of omega concentrate fermented by using normal purified water at a concentration of 500 μg / ml, It is good to know.

Experimental Example 6 Confirmation of Inhibitory Effect on Collagenase (MMP-1)

In order to measure the promoting effect of the collagen synthesis promoting effect of the concentrate of the lava sea water omega-concentrate of Example 1 and the concentrate of the omega-concentrate of the purified water of Comparative Example 1, a commercially available human fibroblast was treated with a sample. Human normal fibroblasts (CCD-985sk) were mixed at a density of 1 × 10 ⁶ cells / dish with IMDM (Iscove's Modified Dulbesco's Media) medium containing 10% fetal bovine serum in a 60 mm dish, ₂ &lt; / RTI &gt; After 24 hours, the medium was removed, and 3 ml of HBSS (Hank's Balanced salt solution) was added. After treatment with UVA 6 J / cm ² , the sample was diluted with IMDM medium containing no fetal bovine serum TRIzol (Invitrogen, USA). The isolated RNA was quantitated using Qubit fluoremeter with RNA BR Assay kit and cDNA (complementary DNA) was synthesized and real-time PCR was performed. For cDNA synthesis, cDNA synthesis kit (High Capacity RNA to cDNA Kit, Applied Biosystems, USA) was used. Real-Time PCR: 7500 Fast with Power SYBR Green PCR Master Mix (Applied Biosystems, USA) was used to amplify the gene and quantitatively analyze the amplified product. The MMP-1 and β-Actin primers used in the PCR were synthesized and used in Cosomjin TEX (Korea). The primer sequences are shown in Table 9, and the experimental results are shown in Table 10 and FIG.

Condition Collagenase (MMP-1) beta -actin Sense 5'-CCA CCT ATC ACT TCA GAT CAA CAA A-3 ' 5'-GGC ACC CAG CAC AAT GAA G-3 ' Antisense 5'-TTC CTG TAG GCC AGT GTC AAA AT-3 ' 5'-CCG ATC CAC ACG GAG TAC TTG-3 '

Sample Name Average Stdev. Control 27.80 1.66 UVA (6 J / cm ² ) 100 0 UVA (6 J / cm ² ) + EGCG 5 μg / ml 56.78 3.21 UVA (6J / cm ²⁾ + Example 1 (500 ㎍ / ㎖) 74.78 5.81 UVA (6 J / cm ² ) + Comparative Example 1 (500 쨉 g / ml) 93.68 3.14

The expression of the MMP-1 gene in the concentrate of the lava sea water omega-concentrate of Example 1 and the concentrate of the omega-concentrate of the purified water of Comparative Example 1 was confirmed. The concentrate of the lava sea water omega- 1 activity. Thus, it can be seen that the concentrate of the lava sea water omega of Example 1 can be used as a cosmetic composition having excellent anti-wrinkle effect.

Experimental Example 7. AQP-3 (AQP-3) Expression Test [

In order to measure the moisturizing effect of the concentrate of the lava sea water omega-concentrate of Example 1 and the concentrate of the omega-concentrate of the purified water of Comparative Example 1, a commercially available human fibroblast was treated with a sample. HaCaT cells were cultured in a 60 mm dish at a density of 1 × 10 ⁶ cells / dish with DMEM supplemented with 10% fetal bovine serum (Dulbecco's modified Eagle's medium) at 37 ° C and 10% CO ₂ for 24 hours. After 24 hours, the samples are diluted in DMEM medium containing no fetal bovine serum and treated for one day. Cells are collected with TRIzol (Invitrogen, USA) to isolate RNA. The isolated RNA was quantitated using Qubit fluoremeter with RNA BR Assay kit and cDNA (complementary DNA) was synthesized and real-time PCR was performed. For cDNA synthesis, cDNA synthesis kit (High Capacity RNA to cDNA Kit, Applied Biosystems, USA) was used. Real-Time PCR: 7500 Fast with Power SYBR Green PCR Master Mix (Applied Biosystems, USA) was used to amplify the gene and quantitatively analyze the amplified product. AQP-3 and β-Actin primers used in PCR were synthesized and used in Bioneer (Korea). The primer sequences are shown in Table 11, and the experimental results are shown in Table 12 and FIG.

Aquaporin-3 (AQP-3) beta -actin Sense 5'-GGG ACC AGT CGG AAG GGA T-3 ' 5'-GGC ACC CAG CAC AAT GAA G-3 ' Antisense 5'-CAC AGA TGG ACA GGC TGC CT-3 ' 5'-CCG ATC CAC ACG GAG TAC TTG-3 '

Sample Name Conc. Average Stdev. Control 100 0 Retinoic acid (nM) 50 124.39 4.82
Example 1 (占 퐂 / ml) 100 127.21 2.03 250 133.62 1.98 500 142.41 1.59
Comparative Example 1 (占 퐂 / ml) 100 108.87 8.45 250 124.07 6.84 500 124.40 6.09

As a result of the AQP-3 gene expression analysis to determine the moisturizing effect of the concentrate of the lava sea water omega-concentrate of Example 1 and the concentrate of the omega-concentrate of the purified water of Comparative Example 1, the AQP- The concentration of omega - concentrate of 100 ㎍ / ㎖ was higher than that of retinoic acid, which was used as a control, and it was found that the expression level of the concentrate was significantly increased. Able to know.

EXPERIMENTAL EXAMPLE 8 Investigation of Protein Expression of filaggrin [

In order to measure the moisturizing effect of the concentrate of the lava sea water omega-concentrate of Example 1 and the concentrate of the omega-concentrate of the purified water of Comparative Example 1, a commercially available human fibroblast was treated with a sample. In a 60-mm dish, DMEM (Dulbecco's modified Eagle's medium) containing 10% fetal bovine serum and HaCaT cells were aliquoted at 1 × 10 ⁶ cells / dish and cultured at 37 ° C. and 10% CO ₂ for 24 hours. After 24 hours, samples are diluted in DMEM medium without fetal bovine serum and cultured for 72 hours. Cells are scraped with a cell scrapper, and the pellet is submerged by centrifugation. Protein extraction solution is added, incubated, and protein is quantified using Qubit ™ fluorometer. Loading buffer is added to the quantified protein, and then loaded on SDS-PAGE. iBlot Dry Blotting system to block after transfer. Transfer paper to the size of the band after the altar (FLG (SC-66192, santacruz)) and second (GAM-HRP (170-6516, Bio-rad)) antibody treatment. The band was photographed with Chemi doc LuminoGraph II (ATTO) after treatment with the antibody, treated with west save cold ECL solution, quantified and quantified, and the results are shown in Table 13 and FIG.

sample conc. (占 퐂 / ml) Quantity value Filaggrin / GAPDH (%) Control One 100.0
Example 1 100 1.234 123.4 250 1.881 188.1 500 2.191 219.1
Comparative Example 1 100 1.027 102.7 250 1.203 120.3 500 1.264 126.4

The amount of the filaggrin protein expressed in the concentrate of the lava sea water omega-concentrate of Example 1 and the concentrate of the concentrate of the omega-concentrate of Comparative Example 1 was checked. As a result, the expression level of the concentrate of the lava seawater omega- It was confirmed that the concentrate had excellent moisturizing effect.

<Production Example of Cosmetics 1. Lotion>

(Skin lotion) of Formulation Example 1 was prepared with the composition shown in the following Table 14, using the material obtained by dissolving the concentrate of Example 1 to 2.0% (w / w) as follows. Glycerin, butylene glycol, propylene glycol, and an antiseptic were added to purified water in this order and stirred to dissolve to obtain a mixed solution. The polyoxyethylene hardened castor oil was dissolved by heating at about 60 DEG C, and then the perfume was added to dissolve and then added to the above mixed solution. Finally, a 2% aqueous solution of the concentrate of Example 1, ethanol, triethanolamine, and pigment were added, stirred sufficiently, and aged. Thus, the final concentration of the example concentrate in Formulation Example 4 was 0.02%, which was prepared at the same concentration in subsequent Formulation Examples 2-6.

In the case of Comparative Formulation Example 1, except for the 2% aqueous solution of the concentrate of Example 1, the remaining amount was added with purified water, followed by sufficiently stirring and aging.

number Raw material Formulation Example 1 (% by weight) Comparative Formulation Example 1 (% by weight) One Example 1 A 2% aqueous solution of the concentrate 2.0 - 2 glycerin 3.0 3.0 3 Butylene glycol 2.0 2.0 4 Propylene glycol 2.0 2.0 5 Polyoxyethylene-enriched free radical 1.0 1.0 6 ethanol 10.0 10.0 7 Triethanolamine 0.1 0.1 8 antiseptic a very small amount a very small amount 9 Pigment a very small amount a very small amount 10 Spices a very small amount a very small amount 11 Purified water To 100 To 100

<Cosmetic Production Example 2. Nutrition Lotion>

The concentrate of Example 1 was dissolved in 2.0% (w / w), and the nutrient lotion of Formulation Example 2 was prepared as follows in the form of the following Table 15, using as a raw material.

Propylene glycol, carboxy polymer, preservative, and purified water were heated to 80 to 85 ° C while being mixed and stirred. Then, the mixture was charged into a manufacturing part, and then an emulsifier was added thereto. Then, wax, polysorbate 60, sorbitan sesquioleate, liquid paraffin, sorbitan stearate Glyceryl monostearate glycerin, stearic acid, glyceryl stearate / phage-400 stearate, and triethanolamine were heated and dissolved at a temperature of 80 to 85 ° C, followed by emulsification. After the emulsification was completed, the mixture was cooled to 50 캜 with stirring using a stirrer, and the fragrance was added thereto. The mixture was cooled to 45 캜 and then added with a dye. A 2% aqueous solution of the concentrate of Example 1 was added thereto at 35 캜, . In the case of Comparative Formulation Example 2, except for the 2% aqueous solution of the concentrate of Example 1, the remaining amount was added with purified water, followed by cooling to 25 캜 and aging.

number Raw material Formulation Example 2
(weight%) Comparative Formulation Example 2
(weight%) One Example 1 A 2% aqueous solution of the concentrate 2.0 - 2 Wax 1.0 1.0 3 Polysorbate 60 1.5 1.5 4 Sorbitan sesquioleate 0.5 0.5 5 Liquid paraffin 10.0 10.0 6 Sorbitan stearate 1.0 1.0 7 Pro-type glycerin monostearate 0.5 0.5 8 Stearic acid 1.5 1.5 9 Clicryl stearate / FAGE-400 stearate 1.0 1.0 10 Propylene glycol 3.0 3.0 11 Carboxy polymer 0.1 0.1 12 Triethanolamine 0.2 0.2 13 antiseptic a very small amount a very small amount 14 Pigment a very small amount a very small amount 15 Spices a very small amount a very small amount 16 Purified water To 100 To 100

<Production Example of Cosmetics 3. Nutritional Cream>

The concentrate of Example 1 was dissolved to 2.0% (w / w), and the nutritional cream of Formulation Example 3 was prepared as follows in the composition shown in Table 16, using as a raw material. Carboxyvinyl polymer, butylene glycol, glycerin, and purified water are heated to a temperature of 80 to 85 ° C while mixing and stirring. Then, the mixture is put into a manufacturing section, and then an emulsifying agent is caused to react with an emulsifying agent such as stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate Glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate, wax, liquid paraffin, squalane, caprylic / capric triglyceride at 80 to 85 ° C to dissolve Triethanolamine was added and stirred into the production part and emulsified. When the emulsification was completed, the mixture was cooled to 35 DEG C with stirring using an agitator, and a 2% aqueous solution of the concentrate of Example 1 was added thereto, followed by cooling to 25 DEG C and aging. In the case of Comparative Formulation Example 3, except for the 2% aqueous solution of the concentrate of Example 1, the remaining amount was added with purified water, followed by cooling to 25 캜 and aging.

number Raw material Formulation Example 3
(weight%) Comparative Formulation Example 3
(weight%) One Example 1 A 2% aqueous solution of the concentrate 2.0 - 2 Stearic acid 2.0 2.0 3 Cetyl alcohol 2.0 2.0 4 Glyceryl monostearate 2.0 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 0.5 6 Sorbitan sesquioleate 0.5 0.5 7 Glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate 1.0 1.0 8 Wax 1.0 1.0 9 Liquid paraffin 4.0 4.0 10 Squalane 4.0 4.0 11 Caprylic / capric triglyceride 4.0 4.0 12 Carboxyvinyl polymer 0.3 0.3 13 Butylene glycol 5.0 5.0 14 glycerin 3.0 3.0 15 Triethanolamine 0.5 0.5 16 Purified water To 100 To 100

<Manufacturing Example of Cosmetics 4. Massage Cream>

Example 1 Massage creams of Formulation 4 containing a 2% aqueous solution composition of a concentrate were prepared as follows in the composition of Table 17 below. The mixture of carboxyvinyl polymer, butylene glycol, glycerin, antiseptic and purified water was mixed and stirred at 80 to 85 ° C., and the mixture was put into a manufacturing part. Then, an emulsifying agent was added thereto and wax, polysorbate 60, sorbitan sesquioleate, Fins, squalane, and glycerin monostearate having a proton type were heated to 80 to 55 ° C and emulsified. After the emulsification was completed, the mixture was cooled to 50 ° C with stirring using an agitator, and then the perfume was added thereto. After cooling to 45 ° C, the dye was added. When the mixture was cooled to 35 ° C, 2% aqueous solution of the concentrate of Example 1 was added, And then aged. In the case of Comparative Formulation Example 4, it was prepared in the same manner as in Formulation Example 4 except that the 2% aqueous solution of the concentrate of Example 1 was omitted.

number Raw material Formulation Example 4
(weight%) Comparative Formulation Example 4
(weight%) One Example 1 A 2% aqueous solution of the concentrate 2.0 - 2 Wax 10.0 10.0 3 Polysorbate 60 1.5 1.5 4 Sorbitan sesquioleate 0.8 0.8 5 Liquid paraffin 40.0 40.0 6 Squalane 5.0 5.0 7 Pro-type glycerin monostearate 1.0 1.0 8 Carboxyvinyl polymer 0.2 0.2 9 Butylene glycol 6.0 6.0 10 glycerin 6.0 6.0 11 Triethanolamine 0.2 0.2 12 antiseptic a very small amount a very small amount 13 Pigment a very small amount a very small amount 14 Spices a very small amount a very small amount 15 Purified water To 100 To 100

<Cosmetic Production Example 5. Essence>

Example 1 The essence of Formulation 5 containing a 2% aqueous solution composition of a concentrate was prepared as follows in the composition of Table 18 below. Sitosterol, polyglyceryl 2-olate, ceramide, stearase-4 and cholesterol were homogenized at a constant temperature to be referred to as nonionic amphipathic lipids. The non-ionic amphiphilic lipids were mixed with the extract powder of Example 1, dicetyl phosphate, concentrated glycerin and purified water, homogenized at a constant temperature, passed through a microfluidizer, and then macadamia oil was gradually added at a constant temperature to homogenize And then passed again to the microfluidizer. Then, carboxyvinyl polymer, xanthan gum, preservative and perfume were added, dispersed, stabilized and aged. After the emulsification was completed, the perfume was added thereto. The mixture was cooled to 45 캜, and the dye was added. When the mixture was cooled to 35 캜, a 2% aqueous solution of the concentrate of Example 1 was added thereto and then cooled to 25 캜. Comparative Example 5 was prepared in the same manner as in Formulation Example 5 except that the 2% aqueous solution of the concentrate of Example 1 was omitted.

number Raw material Formulation Example 5
(weight%) Comparative Formulation Example 5
(weight%) One Example 1 A 2% aqueous solution of the concentrate 2.0 - 2 Sitosterol 1.7 1.7 3 Polyglyceryl 2-oleate 1.5 1.5 4 Ceramide 0.7 0.7 5 Steareth-4 1.2 1.2 6 cholesterol 1.5 1.5 7 Dicetyl phosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Macadamia oil 15.0 15.0 10 Carboxyvinyl polymer 0.2 0.2 11 Xanthan gum 0.2 0.2 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water To 100 To 100

<Cosmetic Production Example 6. Emulsion type foundation>

The emulsified type foundation of Formulation Example 6 containing a 2% aqueous solution of the concentrate of Example 1 in which the extract of Example 1 was dissolved was prepared as follows in the composition of Table 19 below. And the mixture is heated to 50 to 55 ° C while stirring, and then the resulting mixture is reacted with an emulsifying agent and reacted with beeswax, cyclomethicone, polyvinylpyrrolidone, Liquid paraffin, squalane, stearic acid, lipophilic monostearic acid glycerin, and macadamia oil are heated to between 50 and 55 ° C and emulsified. When the emulsification is completed, the mixture is cooled to 45 ° C with stirring using an agitator, and the fragrance is added. After cooling to 35 ° C, a 2% aqueous solution of the concentrate of Example 1 is added and cooled to 25 ° C to be aged. Comparative Example 6 was prepared in the same manner as in Formulation Example 6 except that the 2% aqueous solution of the concentrate of Example 1 was omitted.

number Raw material Formulation Example 6
(weight%) Comparative Formulation Example 6
(weight%) One Example 1 A 2% aqueous solution of the concentrate 2.0 - 2 Wax 2.0 2.0 3 Cyclomethicone 2.0 2.0 4 Flow parison pin 5.0 5.0 5 Squalane 5.0 5.0 6 Stearic acid 2.0 2.0 7 Lipophilic monostearic acid glycerin 3.0 3.0 8 Macadamia oil 4.0 4.0 9 glycerin 4.0 4.0 10 Propylene glycol 3.0 3.0 11 Butylene glycol 3.0 3.0 12 Triethanolamine 1.0 1.0 13 Aluminum magnesium silicate 0.5 0.5 14 Pigment 12.0 12.0 15 antiseptic a very small amount a very small amount 16 Spices a very small amount a very small amount 17 Purified water To 100 To 100

<Experimental Example 9> Measurement of improvement effect of skin elasticity by reduction of wrinkle depth>

Formulation Example 3 and Comparative Formulation Example 3 were divided into two groups of 20 females in order to test the effect of containing omega-concentrate fermented using lava waters against skin wrinkles. After cleansing, it was applied twice in the morning and evening. The nutritional cream of Comparative Formulation Example 3 (Comparative Formulation Example 3) was used as a control group at the right eye margin. After 15 days and 30 days of application, the skin wrinkle depth on the skin surface was measured using Cutometer SEM 474. The skin elasticity measurement by Cutometer SEM 474 measures the depth of the wrinkles. The smaller the value, the better the resilience. Table 20 shows the average values of the elasticity of the 20 testees.

sample Skin elasticity (reduced wrinkle depth) Effect (%) 0 days 15th 30 days Formulation Example 3 0 7.5 25.3 Comparative Formulation Example 3 0 2.4 9.4

As a result of the measurement, as shown in Table 20, the cosmetic product containing the omega concentrate fermented with lava sea water (Formulation Example 3) was 28.3% after 30 days of application, And 9.4%, respectively, the wrinkle suppressing effect is excellent.

Claims (10)

A cosmetic composition comprising, as an active ingredient, a concentrate obtained by removing alcohol from omega fermented by using lava sea water,
The omega starch fermented by using the lava water,
Heating the millet with steam at 90 to 120 캜 for 10 to 30 minutes to prepare and cooling the cooked rice;
In the formula, 100 parts by weight of high- And 20 to 40 parts by weight of yeast are added and fermented at 25 to 28 DEG C for 40 to 50 hours;
200 to 400 parts by weight of new capsicum and 100 to 160 parts by weight of fresh lava seawater were added to 100 parts by weight of the above foundation and fermented with stirring at 25 to 28 ° C for 40 to 50 hours to prepare an explant. And then adding the diatomaceous earth to the aged skin and filtering the skin with a filter. The cosmetic composition for improving the skin's wrinkles, improving elasticity or moisturizing skin. delete delete delete The method according to claim 1,
Wherein the concentrate is effective for inhibiting the activity of elastase or collagenase. The cosmetic composition for improving the wrinkles, elasticity, or moisturizing properties of the skin. The method according to claim 1,
Wherein the concentrate is effective for increasing the expression of AQP-3 or filaggrin. A cosmetic composition for improving the wrinkles, elasticity or moisturizing properties of the skin, which comprises the cosmetic composition according to any one of claims 1, 5 and 6. 8. The method of claim 7,
Characterized in that 0.001 to 20% by weight of omega-concentrate is contained in the cosmetic composition based on 100% by weight of the total cosmetics. 8. The method of claim 7,
The cosmetic may be at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizer cream, a hand cream, a foundation, , A cleansing lotion, a cleansing cream, a body lotion, and a body cleanser. delete

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