KR102284899B1 - Cosmetic composition comprising mature peptides of Astragalus membranaceus for improving skin vitality - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin vitality containing an Astragalus propinquus peptide aged product, and as a low molecular weight peptide composition, obtained by proteolytically decomposing Astragalus roots and aging the roots in a Scutellaria baicalensis aging tank, of the present invention has excellent cell vitality and anti-wrinkle effects, the composition can be easily used as the cosmetic composition for skin improvement.

Description

Cosmetic composition comprising mature peptides of Astragalus membranaceus for improving skin vitality

The present invention relates to a cosmetic composition for improving skin vitality containing an aged astragalus-derived peptide having a molecular weight of 10,000 da or less.

The skin is a body organ that is in close contact with the external environment in the human body and has a function of protecting the inside of the human body, and can be roughly divided into three types: the epidermis, the dermis, and the hypodermis. The skin is a complex organ composed of cells with very diverse functions and materials with appropriate properties. The dermis layer maintains the original skin elasticity because elastic solid materials (fibers) and viscous liquid materials are combined. . Thus, the skin exhibits a reaction due to its unique physical properties to mechanical tension, which is called viscoelasticity. The reason that the skin has such viscoelasticity is because collagen fibers and elastin fibers form a unique three-dimensional structure in the matrix of proteoglycans. In general, as we age, the function of the skin deteriorates and atrophic changes occur due to ultraviolet rays, pollution, stress, etc., and the number of skin cells decreases or the thickness of the skin decreases. In this process, the elastic fibers of the skin are deformed, and the elasticity of the skin is reduced or the skin is stretched. Collagen is a major component constituting the skin, of which type I collagen accounts for 80% and type III collagen accounts for 15%. When the skin is exposed to ultraviolet rays for a long time, a large amount of reactive oxygen species is generated in the skin, resulting in the expression of matrix metalloproteinase-1 (MMP-1), an enzyme that degrades collagen among matrix metalloproteinases (MMPs), or The activity is promoted and collagen synthesis is lowered, which in turn leads to a decrease in skin elasticity and the creation of wrinkles.

Therefore, in order to prevent skin aging and damage caused by these internal and external factors to maintain healthier and more beautiful skin, various physiologically active substances are added through cosmetics to maintain the unique function of the skin and activate skin cells. Efforts to improve skin vitality by promoting skin metabolism are requested.

Astragalus membranaceus BUNGE (Astragalus membranaceus BUNGE) is a perennial herbaceous plant belonging to the legume family. The root is used as a medicine, and the active ingredients are folic acid and choline. It has excitatory action and diuretic action on the central nervous system, suppresses the occurrence of nephritis, delays the occurrence of proteinuria and cholesterol, and lowers blood pressure. This medicine is mild in weakness and sweet in taste. It has been widely used as a substitute for ginseng since ancient times because it shows remarkable efficacy for people who are easily tired, weak, have a low voice, have a weak pulse, and sweat a lot. It is also widely used for uterine effusion, gastric pitting, redness, and uterine bleeding, and it also promotes physical strength and increases the tension of the whole body muscles. Therefore, it is one of the most widely used medicines in Chinese medicine. However, it cannot be used when the fever is severe. In folklore, it is widely used because it is said that if you put this medicine in chicken and eat it for a month, you will not break a cold sweat and your stamina will be strengthened. A typical prescription is Bojungikgi-tang, which is often used for people who have no energy and have lost their appetite.

On the other hand, the present inventors selected astragalus as a composition that can improve skin vitality and used a proteolytic method as a method to process it, and the proteolytically obtained composition has excellent efficacy in enhancing the activity of mitochondria in skin cells By confirming the present invention was completed.

Republic of Korea Patent Registration No. 10-1663030 (Title of the invention: Cosmetic composition for preventing skin aging containing Astragalus peptide, Applicant: Cosmeca Korea Co., Ltd., Registration date: September 29, 2016) Republic of Korea Patent Registration No. 10-1415993 (Title of the invention: Composition for external application for skin containing Astragalus extract extracted from an extractor equipped with Onggi as an active ingredient, Applicant: AMOREPACIFIC, Registration date: July 01, 2014) Republic of Korea Patent No. 10-0858629 (Title of the invention: Cosmetic composition for enhancing skin vitality containing cypress extract, sea pine extract and white pine extract as active ingredients, Applicant: Koreana Cosmetics Co., Ltd., Registration date: September 09, 2008) Republic of Korea Patent No. 10-1219683 (Title of the invention: Aspergillus oryase and Astragalus fermented cereals having tyrosinase inhibitory activity and manufacturing method thereof, Applicant: Konkuk University Industry-Academic Cooperation Foundation, Registration date: 2013 January 02, 2017) Republic of Korea Patent Publication No. 10-2020-0074361 (Title of the invention: Composition for skin whitening and UV protection composition containing extract of fermented Astragalus fermented Astragalus as an active ingredient, Applicant: Republic of Korea (Director of Rural Development Administration), Publication date : June 25, 2020)

It is an object of the present invention to provide a cosmetic composition for improving skin vitality containing an aged astragalus-derived peptide having a molecular weight of 10,000 da or less. Another object of the present invention is to provide a cosmetic composition having a skin regeneration, wrinkle improvement or moisturizing function containing the aged astragalus-derived peptide.

The present invention relates to a cosmetic composition containing Astragalus membranaceus BUNGE (Astragalus membranaceus BUNGE) peptide.

The peptide is characterized in that it has a molecular weight of 10,000 da or less.

The cosmetic composition is characterized in that it has a skin vitality improvement function.

Additionally, the cosmetic composition is characterized in that it has a skin regeneration, wrinkle improvement or moisturizing effect.

The composition enhances energy activity in mitochondria, and has an effect of increasing the expression of PGC-1α (Peroxisome proliferator-activated receptor gamma co-activator 1α), collagen type I, or elastin.

The astragalus peptide aged product is characterized in that it is prepared by the following method.

Preferably, (first step) performing primary proteolysis after adding water to Astragalus root and treating a mixture of pectinase and cellulase;

(Second step) performing secondary proteolysis by further treating with proteinase;

(Step 3) filtering the enzyme-treated product obtained after secondary proteolysis to obtain a low molecular weight peptide composition; and,

(Step 4) Aging in a golden aging tank after concentrating the low molecular weight peptide composition;

It can be prepared including.

The low molecular weight peptide composition of the third step is characterized in that it has a molecular weight of 10,000 da or less. Preferably, the composition has a molecular weight of 100 to 10,000 da.

In addition, in step 4, the low molecular weight peptide composition may be concentrated to a solid content of 80% by weight or more.

It is preferable to ripen for 70 to 100 days in the golden aging tank in step 4.

500 to 3000 parts by weight of water is preferably added based on 100 parts by weight of the astragalus root, and 0.5 to 3 parts by weight of each of the pectinase, cellulase and proteinase is preferably added based on 100 parts by weight of the astragalus root. At this time, it is better to use dried Astragalus roots and can be prepared by cutting them into 4-6cm lengths. The first proteolysis is preferably performed at about 37-42° C. for 18-30 hours. Secondary proteolysis is preferably performed at about 47-52 ° C for 18-30 hours. If these conditions are exceeded, the activity of each enzyme in the proteolysis process of Astragalus is not appropriate and proteolysis is not desired, so it may be difficult to obtain a peptide composition in an appropriate state.

Concentration after enzymatic treatment of the peptide is preferably performed by a reduced pressure concentration method.

The golden aging tank used in the present invention is characterized in that it is a container that is gold-plated inside, is made of porcelain material that is not glazed, and is in a state in which the peptide composition can be stored by closing the lid.

Hereinafter, the present invention will be described in detail.

In addition, the present invention provides a pharmaceutical composition for improving skin vitality containing astragalus peptide aged.

The pharmaceutical composition may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., oral dosage forms, external preparations, suppositories, and sterile injection solutions according to conventional methods, respectively. Carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, it is prepared using diluents or excipients, such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and such solid preparations include at least one excipient, for example, starch, calcium carbonate, sucrose or It is prepared by mixing lactose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid formulations for oral use include suspensions, solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, fragrances, preservatives, etc. may be included. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, and the like can be used.

The dosage of the pharmaceutical composition of the present invention will vary depending on the age, sex, and weight of the subject to be treated, the specific disease or pathological condition to be treated, the severity of the disease or pathological condition, the route of administration, and the judgment of the prescriber. Dosage determination based on these factors is within the level of the skilled artisan, and dosages generally range from 0.01 mg/kg/day to approximately 2000 mg/kg/day. A more preferred dosage is 1 mg/kg/day to 500 mg/kg/day. Administration may be administered once a day, or may be administered in several divided doses. The above dosage does not limit the scope of the present invention in any way.

The pharmaceutical composition of the present invention may be administered to mammals such as mice, livestock, and humans by various routes. Any mode of administration can be envisaged, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebrovascular injection. Since the extract of the present invention has almost no toxicity and side effects, it is a drug that can be safely used even when taken for a long period of time for the purpose of prevention.

In addition, the present invention provides a health functional food for improving skin vitality, comprising astragalus peptide aging composition and food-logically acceptable food supplement additives. The health functional food of the present invention includes the form of tablets, capsules, pills, or liquids, and the food to which the extract of the present invention can be added includes, for example, various drinks, meat, sausage, bread, candy, Snacks, noodles, ice cream, dairy products, soups, ionized beverages, beverages, alcoholic beverages, gum, tea, and vitamin complexes.

In the formulation of the cosmetic composition of the present invention, It can be prepared in any formulation conventionally prepared in the art, and essence, lotion, emulsion, pack, hand cream, foot cream, lip balm, lipstick, eye shadow, eyeliner, eyebrow pencil, blusher, highlighter, general Lotion, skin, cream, serum, beauty soap, flexible lotion, medicinal lotion, whole body cleanser, cleansing foam, cleansing lotion, gel, cleansing oil, cleansing cream, shampoo, conditioner, hair treatment, hair lotion, cleansing tissue and cleansing water You can provide what is selected from.

The cosmetic composition of the present invention may further include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extract.

The water-soluble vitamin may be any as long as it can be formulated in cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, etc. and their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) are also water-soluble vitamins that can be used in the present invention. Included. Water-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a microbial culture, an enzymatic method or a chemical synthesis method.

The oil-soluble vitamins may be any as long as they can be formulated in cosmetics, but preferably include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (DL-alpha tocopherol, D-alpha tocopherol, D-alpha tocopherol) and the like. , their derivatives (ascorbine palmitate, ascorbine stearate, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinic acid, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a microbial culture, an enzyme or a chemical synthesis method, and the like.

The polymer peptide may be any compound as long as it can be blended into the cosmetic composition, and preferred examples include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. Polymeric peptides can be purified and obtained by conventional methods such as purification from a microbial culture solution, enzyme method or chemical synthesis method, or can be purified from natural products such as dermis of pigs or cattle, silk fibers of silkworms, and the like.

The high molecular weight polysaccharide may be any substance that can be blended into the cosmetic composition, but preferably hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) is mentioned. For example, chondroitin sulfate or a salt thereof can be purified and used from mammals or fish.

The sphingolipid may be any as long as it can be blended into the cosmetic composition, and ceramide, phytosphingosine, sphingoglycolipid and the like are preferred. The sphingolipid can be purified by a conventional method from mammals, fish, shellfish, yeast or plants, or can be obtained by chemical synthesis.

The seaweed extract may be any one as long as it can be blended into the cosmetic composition, but preferably brown algae extract, red algae extract, green algae extract, etc., and calrageenan purified from these seaweed extracts, arginic acid, sodium alginate , potassium alginate, etc. are also included in the seaweed extract used in the present invention. The seaweed extract can be obtained by purification from seaweed by a conventional method.

The cosmetic composition of the present invention may also contain other ingredients that are formulated in conventional cosmetics.

Other ingredients that may be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, disinfectants, antioxidants, plant extracts, pH adjusters, alcohols, colorants, fragrances, A blood circulation promoter, a cooling agent, a restrictive agent, purified water, etc. are mentioned.

Examples of the fat and oil component include ester fats and oils, hydrocarbon oils and fats, silicone oils and fats, fluorine oils and fats, animal oils and fats, and vegetable oils and fats.

Examples of ester-based oils and fats include glyceryl tri-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, stearic acid. Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine Diisopropyl acid, isoalkyl neopentanoate, tri (caprylic acid, capric acid) glyceryl, trimethylol propane tri-2-ethylhexanoate, trimethylol propane triisostearate, pentaelysitol tetra2-ethylhexanoate , cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isostea laurate Reel, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isostearate isopropyl, 2-ethylhexanoate cetoster Aryl, 2-ethyl stearyl hexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, di(caprylic acid, capric acid) propylene glycol, dicaprylic acid propylene glycol, dicaprylate Neopentyl glycol phosphate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate , octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleate, polyglycerol isostearate, Triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, dimalate Isostearyl, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, Cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 12-stealoyl Ester types, such as isocetyl hydroxy stearate, 12-stealoyl hydroxy stearyl stearate, and 12-stealoyl hydroxy stearate isostearyl, etc. are mentioned.

Examples of hydrocarbon-based oils and fats include hydrocarbon-based oils and fats such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax, petrolatum.

Examples of silicone oils and fats include polymethyl silicone, methylphenyl silicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylstealloxysiloxane copolymer, and alkyl Modified silicone oil, amino modified silicone oil, etc. are mentioned.

Perfluoropolyether etc. are mentioned as fluorine-type fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, saffron oil, soybean oil, corn oil, rapeseed oil, passerine oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil , cottonseed oil, palm oil, kukui nut oil, wheat germ oil, rice germ oil, shea butter, cranberry colostrum, marcadeumia nut oil, meadow home oil, egg yolk oil, tallow oil, horse oil, mink oil, orange rapie oil, jojoba oil , animal or plant oils and fats, such as candelabra wax, carnauba wax, liquid lanolin, and hydrogenated castor oil.

Examples of the moisturizing agent include a water-soluble low-molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

As water-soluble low molecular weight moisturizers, serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n=2 or more), polyglycerol B (polymerization degree n=2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Cholesterol, cholesterol ester, etc. are mentioned as a fat-soluble low molecular weight humectant.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, and dextrin. can

Examples of the fat-soluble polymer include polyvinylpyrrolidone/eicocene copolymer, polyvinylpyrrolidone/hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and high molecular weight silicone.

Examples of the emollient include long-chain acylglutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

Nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, etc. are mentioned as surfactant.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE/POP (polyoxyethylene/polyoxypropylene) copolymer, POE/POP alkyl ether, polyether-modified silicone, lauric acid alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkyl amide sulfate, alkyl phosphate, POE alkyl phosphate, alkyl amide. Phosphate, alkyloylalkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkylsulfosuccinate, alkylsulfoacetate sodium, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. there is.

Examples of the cationic surfactant include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, chloride Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salt of lanolin derivative, etc. are mentioned.

Examples of the amphoteric surfactant include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amidesulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amidamine type, etc. Amphoteric surfactant etc. are mentioned.

Examples of organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide. inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine, and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene/styrene copolymer, and organic pigments such as silk powder, cellulose, CI pigment yellow and CI pigment orange, and composite pigments of these inorganic pigments and organic pigments.

Examples of the organic powder include metal soaps such as calcium stearate; alkyl phosphate metal salts such as sodium cetylate, zinc laurylate, and calcium laurylate; acylamino acid polyvalent metal salts such as calcium N-lauroyl-beta-alanine, zinc N-lauroyl-beta-alanine, and calcium N-lauroyl glycine; amidesulfonic acid polyvalent metal salts such as calcium N-lauroyl-taurine and calcium N-palmitoyl-taurine; N such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylizine, N-alpha-paritoylolnithine, N-alpha-lauroylarginine, N-alpha-hydrogenated beef fatty acid acylarginine, etc. -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, a divinylbenzene-styrene copolymer, ethylene tetrafluoride, etc. are mentioned.

As UV absorbers, para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate, benzyl cinnamate , para-methoxycinnamic acid-2-ethoxyethyl, para-methoxycinnamic acid octyl, dipara-methoxycinnamic acid mono-2-ethylhexane glyceryl, para-methoxycinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, uro Cannic acid, ethyl urokanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenonedisulfonate, dihydroxybenzophenone , tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)-1 and 3,5-triazine and 2-(2-hydroxy-5-methylphenyl)benzotriazole.

Examples of disinfectants include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc phyllithione, benzalkonium chloride, photosensitizer. So, No. 301, mononitroguaial sodium, undecyrenic acid, etc. are mentioned.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and ellisorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmalic acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, blending components that may be added other than this are not limited thereto, and any of the above components can be blended within a range that does not impair the purpose and effect of the present invention, but preferably 0.01 to 5 wt%, more preferably 0.01 to 5 wt%, based on the total weight may be blended in an amount of 0.01 - 3% by weight.

The cosmetic composition of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

The ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetics in addition to the extract as an active ingredient, for example, conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, and carrier.

When the cosmetic composition formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component. can be used

When the cosmetic composition formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon , propane/butane or propellants such as dimethyl ether.

When the cosmetic composition formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol. , 1,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microscopic Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the cosmetic composition formulation of the present invention is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol fatty acid ester and the like can be used.

The present invention relates to a cosmetic composition containing an aged product of Astragalus peptide, wherein the low-molecular-weight peptide composition of the present invention obtained by proteolytically decomposing Astragalus root and aging it in a golden aging tank has excellent cell vitality function and anti-wrinkle effect, It can be easily used as a cosmetic composition for skin improvement.

1 is the result of confirming the cell regeneration efficacy of the aged Astragalus peptide of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided so that this disclosure will be thorough and complete, and will fully convey the spirit of the invention to those skilled in the art.

Example 1. Preparation of Astragalus Peptide Aging Composition

Wash 100 g of dried Astragalus root purchased at Seoul Yangnyeong Market, cut into 5 cm or less, and add 1,000 g of purified water. Here, 1 g of Aspergillus- derived pectinase and 1 g of cellulase were mixed, extracted at 40 °C for 24 hours, and proteolysis was performed. After that, 1 g of Bacillus- derived protease was added and further proteolytically decomposed at 50° C. for 24 hours. The enzyme-treated product obtained by proteolysis was first treated with a filter cloth filtration to obtain a polymer having a molecular weight cutoff of 10,000 to 300,000 da, and then passed through an ultra-filter to obtain a final low molecular weight peptide composition having a molecular weight of 10,000 da or less. Next, the peptide composition was concentrated with a rotary vacuum concentrator to prepare a concentrate having a solid content of 80% by weight or more, and the concentrate was aged in a golden aging tank for 88 days. The golden aging tank used at this time is one in which the inside of the pottery is coated with gold using a harp. The aging temperature was maintained at 1~4℃ or less, and the humidity was maintained at 15% or less.

Example 2. Aging composition prepared by adjusting the aging time to 70 days

In the process of Example 1, the aging period in the golden aging tank was adjusted to 70 days.

Example 3. Aging composition prepared by adjusting the aging time to 100 days

In the process of Example 1, the aging period in the golden aging tank was adjusted to 100 days.

Comparative Example 1. Astragalus peptide aging composition prepared by performing aging in a general container

Aged composition was obtained by performing the aging process in the same manner as in Example 1, except that it was aged using a polyethylene container instead of a golden aging tank.

Comparative Example 2. Astragalus hot water extract

Wash 100 g of dried Astragalus root purchased at Seoul Yangnyeong Market, cut into 5 cm or less, and add 1,000 g of purified water. After that, extraction was carried out for 24 hours without enzymatic treatment. This was filtered with a filter paper and concentrated under reduced pressure to obtain a hot water extract of Astragalus.

Comparative Example 3. Composition without aging

After concentrating the peptide composition after proteolysis and filtration in the process of Example 1, aging was not performed separately, and the concentrate was immediately prepared as an experimental sample.

Comparative Example 4. Composition prepared by performing short aging

In the process of Example 1, the aging period in the golden aging tank was shortened to 40 days. Other than that, the aged product was prepared in the same manner as in Example 1.

Comparative Example 5. Composition i prepared by adjusting the enzyme treatment step

In the process of Example 1, the enzyme mixture of pectinase and cellulase and protease were exchanged for treatment. That is, protease was treated in the first enzymatic treatment step, and an enzyme mixture of pectinase and cellulase was treated in the second enzymatic treatment step. Other than that, the aged product was prepared in the same manner as in Example 1.

Comparative Example 6. Composition ii prepared by adjusting the enzymatic treatment step

In the first enzymatic treatment step of Example 1, an enzyme mixture of pectinase and protease was treated, and in the second enzymatic treatment step, cellulase was treated. Other than that, the aged product was prepared in the same manner as in Example 1.

Comparative Example 7. Composition iii prepared by adjusting the enzymatic treatment step

In the process of Example 1, trypsin and chymotrypsin (10:1, w/w) were used instead of the enzyme mixture of pectinase and cellulase. Other than that, the aged product was prepared in the same manner as in Example 1.

Experimental Example 1. Skin revitalizing confirmation (Mitochondrial biogenesis)

In this experimental example, the skin cell vitality or mitochondrial biosynthesis efficacy of the compositions of Examples 1 to 3 and Comparative Examples 1 to 7 were measured.

Fibroblast cells were inoculated on a cell plate and cultured for 24 hours, washed with HBSS, ^{irradiated with UVB 50 mJ/cm 2} , replaced with serum-free medium, and the sample was treated with cells so that the final concentration was 100 μg/ml. time incubation. Cells were collected with QIAzol™ Lysis Reagent (Qiagen) and RNA was isolated according to the manufacturer's method. After quantifying the isolated RNA, cDNA was synthesized and real-time PCR was performed. This is shown in Table 1 below.

sample (100 μg/ml) Collagen type I expression (%) untreated group 100.0 UVB 50 mJ/cm2 16.0 UVB 50 mJ/cm 2 + Example 1 28.5 UVB 50 mJ/cm 2 + Example 2 26.7 UVB 50 mJ/cm 2 + Example 3 27.1 UVB 50 mJ/cm 2 + Comparative Example 1 22.1 UVB 50 mJ/cm 2 + Comparative Example 2 21.4 UVB 50 mJ/cm 2 + Comparative Example 3 23.4 UVB 50 mJ/cm 2 + Comparative Example 4 22.2 UVB 50 mJ/cm 2 + Comparative Example 5 19.3 UVB 50 mJ/cm 2 + Comparative Example 6 23.0 UVB 50 mJ/cm 2 + Comparative Example 7 22.9 UVB 50 mJ/cm 2 + EGCG (2.5 μg/ml) 24.6

As confirmed in Table 1, it was confirmed that the compositions of Examples 1 to 3 had excellent efficacy in increasing the expression of PGC-1α compared to the compositions of Comparative Examples 1 to 7, and by increasing the expression of PGC-1α, which decreases with age, It can be said that it is a cosmetic composition that helps skin vitality by creating young mitochondria and inducing energy generation.

Experimental example 2. JC -1 Confirmation of cell activation effect through staining

To verify the change in mitochondrial cell membrane potential, keratinocytes (human keratinocytes) were dispensed in a 35 mm plate and stabilized for 24 hours, then replaced with a serum-free medium and the sample was processed. After 1 hour, after washing with HBSS (Hank's Balanced Salt solution) buffer, fresh HBSS was added, and UVB 50 mJ/cm 2 was irradiated. After replacing the medium with serum-free medium, the sample was treated and _{incubated at 37° C., 5% CO 2 in an} incubator for 24 hours. After removal of the medium, 1 μg/ml of JC-1 solution was treated and _{reacted for 2 hours in a 37° C., 5% CO 2} incubator in the absence of light, and then the medium was removed and observed with a fluorescence microscope.

In the case of healthy mitochondria, the ratio of Red:Green is confirmed to be 1:1 through staining using the JC-1 solution, but if the stress that can induce mitochondrial dysfunction is applied or when cell damage or death is induced, Red As with the :Green ratio of 0.5:1, there is no change in the Green value, and the purely Red value is significantly reduced. On the other hand, the experimental results are numerically shown in Table 2.

sample (50 μg/ml) Cell activation effect (%) untreated group 100.0 UVB 50 mJ/cm2 78.0 UVB 50 mJ/cm 2 + Example 1 94.5 UVB 50 mJ/cm 2 + Example 2 92.3 UVB 50 mJ/cm 2 + Example 3 91.2 UVB 50 mJ/cm 2 + Comparative Example 1 79.2 UVB 50 mJ/cm 2 + Comparative Example 2 76.4 UVB 50 mJ/cm 2 + Comparative Example 3 83.0 UVB 50 mJ/cm 2 + Comparative Example 4 84.1 UVB 50 mJ/cm 2 + Comparative Example 5 82.3 UVB 50 mJ/cm 2 + Comparative Example 6 78.9 UVB 50 mJ/cm 2 + Comparative Example 7 82.3 UVB 50 mJ/cm2 + Adenosine (7㎍/㎖) 88.4

As a result, it is confirmed that the compositions of Examples 1 to 3 inhibit the damage of mitochondria increased through UVB damage and maintain the mitochondria in a more healthy state.

Experimental Example 3. Confirmation of cell regeneration ability effect through wound healing test

Fibroblasts were inoculated into a 60 mm plate and cultured for 24 hours. After incubation, a part of the cell plate was scraped in a straight line and the sample was treated by concentration while replacing it with a serum-free medium. After culturing for 3 days, the difference was visually confirmed by photographing the cells under a microscope (Leica). Representatively, a photograph is taken and shown in FIG. 1 .

As a result, it can be confirmed that the cell regeneration ability of the Example 1 composition-treated group of the present invention is superior to that of the Comparative Example 2 composition.

Experimental Example 4. Confirmation of increase in collagen type I expression

In this experimental example, the skin wrinkle improvement or elasticity efficacy of the compositions of Examples 1 to 3 and Comparative Examples 1 to 7 was measured.

Fibroblast cells were inoculated on a cell plate and cultured for 24 hours, then the cultured cell medium was replaced with a serum free medium, and the sample was treated with the cells so that the final concentration was 50 μg/ml and cultured for 24 hours. Cells were collected with QIAzol™ Lysis Reagent (Qiagen) and RNA was isolated according to the manufacturer's method. After quantifying the isolated RNA, cDNA was synthesized and real-time PCR was performed. This is shown in Table 3 below.

sample (50 μg/ml) Collagen type I expression (%) untreated group 100.0 Example 1 139.6 Example 2 142.2 Example 3 145.2 Comparative Example 1 118.3 Comparative Example 2 121.9 Comparative Example 3 135.2 Comparative Example 4 136.2 Comparative Example 5 120.2 Comparative Example 6 121.3 Comparative Example 7 122.3 Vitamin C 114.6

As a result of confirming the expression of collagen type I in each sample, it can be seen that the expression of collagen type I is significantly increased in the compositions of Examples 1 to 3, thereby having a skin elasticity effect.

Experimental example 5. Elastin Confirmation of increased expression

In this experimental example, the skin wrinkle improvement or elasticity efficacy of the compositions of Examples 1 to 3 and Comparative Examples 1 to 7 was measured.

Fibroblast cells were inoculated on a cell plate and cultured for 24 hours, then the cultured cell medium was replaced with a serum free medium, and the sample was treated with the cells so that the final concentration was 100 μg/ml and cultured for 24 hours. Cells were collected with QIAzol™ Lysis Reagent (Qiagen) and RNA was isolated according to the manufacturer's method. After quantifying the isolated RNA, cDNA was synthesized and real-time PCR was performed. This is shown in Table 4 below.

sample (100 μg/ml) Elastin expression level (%) untreated group 100.0 Example 1 274.8 Example 2 283.4 Example 3 262.3 Comparative Example 1 132.3 Comparative Example 2 101.0 Comparative Example 3 172.3 Comparative Example 4 182.8 Comparative Example 5 192.3 Comparative Example 6 146.0 Comparative Example 7 124.2 Vitamin C 134.5

As a result of confirming the expression of elastin in each sample, it can be seen that the expression of elastin is significantly increased in the compositions of Examples 1 to 3 to have skin elasticity effects.

Experimental example 6. Confirmation of increased HAS-2 expression

In this experimental example, the skin moisturizing effect of the compositions of Examples 1 to 3 and Comparative Examples 1 to 7 was measured.

Fibroblast cells were inoculated on a cell plate and cultured for 24 hours, then the cultured cell medium was replaced with a serum-free medium, and the sample was treated with the cells so that the final concentration was 100 μg/ml and cultured for 24 hours. Cells were collected with QIAzol™ Lysis Reagent (Qiagen) and RNA was isolated according to the manufacturer's method. After quantifying the isolated RNA, cDNA was synthesized and real-time PCR was performed. This is shown in Table 5 below.

sample (100 μg/ml) HAS-2 expression level (%) untreated group 100.0 Example 1 144.0 Example 2 156.2 Example 3 162.1 Comparative Example 1 111.2 Comparative Example 2 100.1 Comparative Example 3 125.2 Comparative Example 4 123.4 Comparative Example 5 121.2 Comparative Example 6 104.7 Comparative Example 7 123.2

As a result of confirming the expression of HAS-2 related to moisturizing in each sample, it can be seen that the value is significantly increased in the compositions of Examples 1 to 3.

Experimental example 7. Effect of promoting cellular energy activity

This experiment was conducted using the samples obtained in Examples 1 to 3 and Comparative Examples 1 to 7. The experiment proceeded as follows. ^{First, normal human fibroblasts were inoculated to 1×10 6} cells in each well of a 12-well microplate (Nunc, Denmark), and cultured in DMEM medium (Sigma, USA) at 37° C. for 24 hours. Subsequently, the experiment in which each composition was replaced with a serum-free DMEM medium at a final concentration of 100 μg/ml, and the control group replaced with a serum-free DMEM medium without each extract were further cultured for 48 hours. . After culturing, cells were obtained from each well, and the degree of energy activity in each cell was measured using a cell energy assay kit (ATP assay kit, Amersham, USA). The energy activity promoting effect of each extract is shown in Table 6 below.

sample (100 μg/ml) Skin cell energy activation effect (%) untreated group 100.0 Example 1 176.3 Example 2 183.0 Example 3 163.9 Comparative Example 1 132.3 Comparative Example 2 124.2 Comparative Example 3 135.3 Comparative Example 4 134.4 Comparative Example 5 136.0 Comparative Example 6 121.5 Comparative Example 7 102.2

As a result of confirming the HAS-2 expression in each sample, it can be seen that the HAS-2 expression is significantly increased in the compositions of Examples 1 to 3 to have a skin moisturizing effect.

cosmetics manufacturing example One. skin

Using the raw material of the aged product of Example 1 dissolved in 1,3-butylene glycol to 2.0% (w/w), the skin was prepared with the composition shown in Table 7 below as follows. Glycerin, butylene glycol, propylene glycol, and a preservative were sequentially added to purified water, stirred and dissolved to obtain a mixed solution. Polyoxyethylene hydrogenated castor oil was heated to about 60° C. to dissolve, and then a fragrance was added to dissolve it, and then added to the mixture. Finally, Example 1 aged 2% (w/w) aqueous solution, ethanol, triethanolamine, and a dye were added, stirred sufficiently, and then aged.

Raw material weight% Example 1 2% (w/w) aqueous solution of aged water 2.0 glycerin 3.0 butylene glycol 2.0 propylene glycol 2.0 Polyoxyethylene fortified perm oil 1.0 ethanol 10.0 triethanolamine 0.1 antiseptic a very small amount pigment a very small amount Spices a very small amount Purified water To 100

Cosmetics Preparation Example 2. Nutritional Lotion

A nutrient lotion was prepared as follows with the composition shown in Table 8 below, using the aged product of Example 1 dissolved in 1,3-butylene glycol to 2.0% (w/w) as a raw material.

Propylene glycol, carboxypolymer, preservative, and purified water are mixed and stirred at 80 ~ 85°C and heated to between 80 and 85°C, put into the manufacturing unit, and then the emulsifier works, beeswax, polysorbate 60, sorbitan sesquioleate, liquid paraffin, sorbitan stearate , lipophilic monostearic acid glycerin, stearic acid, glyceryl stearate/PEG-400 stearate, and triethanolamine were dissolved by heating between 80 and 85° C. and then emulsified. After the emulsification is finished, the mixture is cooled to 50° C. while stirring using a stirrer, and then a fragrance is added, a color is added after cooling to 45° C., and a 2% (w/w) aqueous solution of the aged product of Example 1 is added at 35° C. to 25° C. It was then cooled and aged.

Raw material weight% Example 1 2% (w/w) aqueous solution of aged water 2.0 beeswax 1.0 Polysorbate 60 1.5 Sorbitan Sesquiolate 0.5 liquid paraffin 10.0 Sorbitan Stearate 1.0 lipophilic monostearate glycerin 0.5 stearic acid 1.5 glyceryl stearate/PEG-400 stearate 1.0 propylene glycol 3.0 carboxy polymer 0.1 triethanolamine 0.2 antiseptic a very small amount pigment a very small amount Spices a very small amount Purified water To 100

Cosmetic Preparation Example 3. Cream

A nutritious cream was prepared as follows with the composition of Table 9 below, using as a raw material the aged product of Example 1 dissolved in 1,3-butylene glycol to 2.0% (w/w). Carboxyvinyl polymer, butylene glycol, glycerin, and purified water are mixed and stirred at 80~85°C and heated to between 80 and 85°C, put into the manufacturing unit, and then the emulsifier works, stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate Rate, sorbitan sesquioleate, glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate, wax, liquid paraffin, squalane, caprylic / capric triglyceride by heating between 80 ~ 85 ℃ to dissolve Triethanolamine was added and stirred to put into the production unit and emulsified. After the completion of emulsification, the mixture was cooled to 35° C. while stirring using a stirrer, and a 2% (w/w) aqueous solution of the aged product of Example 1 was added, cooled to 25° C., and then aged.

Raw material content (g) Example 1 2% (w/w) aqueous solution of aged water 2.0 stearic acid 2.0 cetyl alcohol 2.0 Glyceryl Monostearate 2.0 Polyoxyethylene sorbitan monostearate 0.5 Sorbitan sesquioleate 0.5 Glyceryl Monostearate/Glyceryl Stearate/Polyoxyethylene Stearate 1.0 wax 1.0 liquid paraffin 4.0

Claims (9)

Astragalus membranaceus BUNGE) A cosmetic composition comprising an aged peptide. According to claim 1,
A cosmetic composition, characterized in that the composition has a skin vitality improvement function. 3. The method of claim 2,
Cosmetic composition, characterized in that the composition has a function of promoting energy activity in mitochondria. According to claim 1,
Cosmetic composition, characterized in that the composition has skin regeneration, wrinkle improvement or moisturizing effect. (First step) adding water to Astragalus root and performing primary proteolysis after treatment with a mixture of pectinase and cellulase;
(Second step) performing secondary proteolysis by further treating with proteinase;
(Step 3) filtering the enzyme-treated product obtained after secondary proteolysis to obtain a low molecular weight peptide composition; and,
(Step 4) Aging in a golden aging tank after concentrating the low molecular weight peptide composition;
Astragalus membranaceus BUNGE (Astragalus membranaceus BUNGE), characterized in that it is prepared, including a method for producing a mature peptide. 6. The method of claim 5,
The low-molecular-weight peptide composition of the third step is a method for producing a mature Astragalus peptide, characterized in that it is a peptide having a molecular weight of 10,000 da or less. 6. The method of claim 5,
A method for producing a mature Astragalus peptide, characterized in that the low molecular weight peptide composition in step 4 is concentrated to 80% by weight or more of the solid content. 6. The method of claim 5,
Method for producing astragalus peptides, characterized in that the aging for 70 to 100 days in the golden aging tank in step 4. A cosmetic composition, characterized in that prepared by any one of claims 5 to 8.

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