KR20240081889A - Composition for improving skin comprising fermented Orostachys japonica as an active ingredient - Google Patents

Abstract

The present invention relates to a fermented product obtained by fermenting Orostachys japonica extract using microorganisms, which has excellent antioxidant effects and superior skin improvement effects, so it can be used as a cosmetic composition, health functional food composition, feed or feed additive composition. You can.

Description

Composition for improving skin comprising fermented Orostachys japonica as an active ingredient}

The present invention relates to a composition for improving skin containing fermented Japanese pine nuts as an active ingredient.

Signs of skin aging, such as a decrease in skin elasticity, dullness and fine wrinkles due to skin peroxidation, that appear with age are a very big concern for women, and there has been great interest in delaying or solving the problem.

Due to this problem, over the past 10 years, it has become a big concern for most women to keep their skin younger and maintain the clean and smooth skin they had in their late teens and early 20s, and the age is gradually getting younger, and even As men also began to take interest in improving their skin condition and preventing aging, interest in and demand for not only ingredients with excellent anti-aging effects but also cosmetics containing them increased explosively, leading to an explosion in anti-aging worldwide. Functional cosmetics that improve wrinkles and elasticity occupy a large portion of the skin care market.

Meanwhile, the skin is largely divided into three layers, from the outside to the epidermis, dermis, and subcutaneous tissue, and has the function of protecting the internal organs from changes in temperature and humidity, ultraviolet rays, and other physical and chemical stimuli from the external environment. there is. In particular, the epidermis plays an important role in preventing moisture evaporation from inside the human body. The epidermis is divided into the stratum corneum, stratum granulosum, stratum spinosum, and basal layer in order from the outside. The cells of the stratum corneum act like bricks, and the intercellular lipids between keratinocytes act like mortar to form the skin barrier. In addition, the keratinocytes of healthy people contain a high concentration of Natural Moisturing Factor (NMF), which helps the skin retain moisture. For example, substances such as amino acids are water-soluble, so they effectively bind with moisture and retain moisture in the skin. Prevents drying out.

However, these days, artificial temperature control of cooling/heating due to environmental changes or changes in lifestyle patterns, skin stress due to various stresses and environmental pollution that occur in social life, frequent face washing due to makeup habits, and natural skin damage due to age increase. The need for skin moisturizers is increasing because the moisture in the stratum corneum decreases due to various causes such as skin aging, which causes the skin to dry out, the surface to become rough, the skin to become dull, lose its luster, and appear dull.

In order to solve the above problems, the present invention confirmed the excellent antioxidant, skin barrier, and moisturizing effects on the skin by fermenting Japanese pine needles, and provides a cosmetic composition for improving skin, health functional food, feed, or feed additive composition using the same. I want to do it.

10-2006-0082784

The present invention seeks to provide a cosmetic composition for improving skin using fermented Wasong.

In addition, the present invention seeks to provide a health functional food composition for skin improvement using fermented Japanese pine nuts.

In addition, the present invention seeks to provide a feed composition for skin improvement using fermented Wasong.

Lastly, the present invention seeks to provide a feed additive composition for skin improvement using fermented wasabi.

The present invention relates to a cosmetic composition for skin improvement containing a fermented Japanese pine tree as an active ingredient, wherein the fermented Japanese pine tree extract is composed of Bacillus subtilis and Saccharomyces cerevisiae. Provided is a cosmetic composition for improving skin, which is obtained by complex fermentation.

In one embodiment of the present invention, the “Wasong extract” may be extracted with 60 to 100% (v/v) distilled ethanol, but is not limited thereto.

In one embodiment of the present invention, the "Wasong fermented product" may be a complex fermentation of Bacillus subtilis and Saccharomyces cerevisiae at a weight ratio of 1:1 to 10. However, it is not limited to this.

In one embodiment of the present invention, the “composition” may have an antioxidant effect, a skin barrier improvement effect, and a moisturizing effect, but is not limited thereto.

In addition, the present invention relates to a health functional food composition for skin improvement containing fermented Japanese pine needles as an active ingredient, wherein the fermented Japanese pine tree extract contains Bacillus subtilis and Saccharomyces cerevisiae ( Provides a health functional food composition for improving skin, which is complex fermented with Saccharomyces cerevisiae .

In one embodiment of the present invention, the “composition” may have an antioxidant effect, a skin barrier improvement effect, and a moisturizing effect, but is not limited thereto.

In addition, the present invention relates to a feed composition for skin improvement comprising fermented Japanese pine needles as an active ingredient, wherein the fermented Japanese pine needles contain Bacillus subtilis and Saccharomyces cerevisiae extracts. ) Provides a feed composition for skin improvement, which is complex fermented with ).

Lastly, the present invention relates to a feed additive composition for skin improvement containing Japanese Japanese pine fermented product as an active ingredient, wherein the Japanese Japanese Japanese pine fermented product contains Japanese Japanese pine extract as Bacillus subtilis and Saccharomyces cerevisiae ( Provides a feed additive composition for skin improvement, which is complex fermented with Saccharomyces cerevisiae .

The present invention relates to a fermented product obtained by fermenting Orostachys japonica using Bacillus subtilis and Saccharomyces cerevisiae , which improves skin, especially antioxidant and skin It has the effect of improving barrier properties and moisturizing.

Figure 1 is a diagram confirming the DPPH radical scavenging ability to determine the antioxidant effect of fermented Wasong product in an embodiment of the present invention.
Figure 2 is a diagram confirming the ABTS radical scavenging ability to determine the antioxidant effect of fermented Wasong product in an embodiment of the present invention.
Figure 3 is a diagram showing FRAP assay performed to determine the antioxidant effect of fermented Wasong product in an embodiment of the present invention.
Figure 4 is a diagram confirming the increase in antioxidant biomarkers in skin keratinocytes of fermented wasong, according to an embodiment of the present invention.
Figure 5 is a diagram confirming the increase in skin barrier and moisturizing biomarkers in skin keratinocytes of fermented Wasong, according to an embodiment of the present invention.
Figure 6 is a diagram quantifying the increase or decrease in skin barrier and moisturizing biomarkers in skin keratinocytes of fermented Wasong (Aquaporin-3, Claudin-1) in one embodiment of the present invention.
Figure 7 is a diagram quantifying the increase/decrease of skin barrier and moisturizing biomarkers in skin keratinocytes of fermented Wasong (Filaggrin, HAS-3) according to an embodiment of the present invention.
Figure 8 is a diagram confirming the increase in skin barrier and moisturizing biomarkers in skin keratinocytes of Wasong fermented product (fermented with BS:SC at 1:2, 1:5, 1:10) in an embodiment of the present invention. am.

Terminology used in this specification is a term used to appropriately express preferred embodiments of the present invention, and may vary depending on the intention of the user or operator or the customs of the field to which the present invention belongs. Therefore, definitions of these terms should be made based on the content throughout this specification. Throughout the specification, when a part is said to “include” a certain element, this means that it may further include other elements rather than excluding other elements, unless specifically stated to the contrary.

Throughout this specification, '%' used to indicate the concentration of a specific substance is (w/w) % for solid/solid, (w/v) % for solid/liquid, and Liquid/Liquid is (v/v) %.

In one aspect, the present invention relates to a cosmetic composition for skin improvement comprising a fermented Japanese pine tree as an active ingredient, wherein the fermented Japanese pine tree extract is a mixture of Bacillus subtilis and Saccharomyces cerevisiae ( It relates to a cosmetic composition for improving skin, which is complex fermented with Saccharomyces cerevisiae .

" Orostachys japonica " of the present invention is a perennial herb of the dicotyledonous plant Rosaceae Sedum family. It is called Orostachys japonica because its shape growing on roof tiles resembles a pine flower, and is also used as a rock pine.

In the present invention, the “Wasong fermented product” is a complex fermentation of Bacillus subtilis and Saccharomyces cerevisiae at a weight ratio of 1:2 to 10. The fermented Japanese pine needles of the present invention have superior antioxidant effects, skin barrier improvement effects, and moisturizing effects compared to the fermented Japanese pine nuts using Bacillus subtilis or Saccharomyces cerevisiae alone. . In addition, the effect is much better than when Lactobacillus plantarum or Lactobacillus rhamnosus, which are widely used for fermentation, are fermented separately.

In the present invention, the extract can be obtained by extraction and separation from nature using extraction and separation methods known in the art, and "extract" as defined in the present invention is extracted using an appropriate solvent, For example, it includes crude extract, polar solvent soluble extract, or non-polar solvent soluble extract. The appropriate solvent for extracting the extract may be any organic solvent acceptable in cosmetics/food science/pharmaceutics, and water or organic solvents may be used, but are not limited thereto, for example, purified water, methanol. Alcohols with 1 to 4 carbon atoms including Methanol, Ethanol, Propanol, Isopropanol, Butanol, etc., Acetone, Ether, Benzene, chloroform Various solvents such as chloroform, ethyl acetate, methylene chloride, hexane, and cyclohexane can be used individually or in combination. As an extraction method, any one of the following methods can be selected and used: hot water extraction, cold needle extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, and compression. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method.

There is no limitation to the method for producing the extract of the present invention, and any known method can be used. For example, the extract included in the composition of the present invention can be prepared in powder form from the primary extract extracted by the above-described hot water extraction or solvent extraction method by additional processes such as reduced pressure distillation and freeze-drying or spray drying. In addition, the primary extract was further purified into fractions using various chromatographies such as silica gel column chromatography, thin layer chromatography, and high performance liquid chromatography. You can also get it. Therefore, in the present invention, extract is a concept that includes all extracts, fractions, and purifications obtained at each stage of extraction, fractionation, or purification, as well as their dilutions, concentrates, or dried products.

The composition containing the fermented Japanese pine needles of the present invention can be used in various ways for skin improvement, especially antioxidant, skin barrier improvement, moisturizing, etc. Products to which this composition can be added include, for example, cosmetics such as various creams, lotions, skins, and essences, as well as shampoos, rinses, cleansers, face washes, soaps, treatments, packs, and beauty essences.

The cosmetic of the present invention may include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular weight peptides, high molecular weight polysaccharides, sphingolipids, and seaweed extracts.

Water-soluble vitamins may be any that can be mixed into cosmetics, but preferably include vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, and vitamin H. and their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbic acid-2-phosphate, etc.) are also water-soluble vitamins that can be used in the present invention. Included. Water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microbial cultures, enzymatic methods, or chemical synthesis.

Oil-soluble vitamins may be any that can be mixed into cosmetics, but preferably include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), etc. , their derivatives (ascorbine palmitate, ascorbine stearate, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinate, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microbial cultures, enzymatic or chemical synthesis.

The polymer peptide may be any peptide that can be blended into cosmetics, but preferably includes collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. High molecular weight peptides can be purified and obtained by conventional methods such as purification from microbial culture media, enzymatic methods, or chemical synthesis, or they can usually be purified and used from natural products such as dermis of pigs or cows or silk fiber of silkworms.

The polymer polysaccharide may be any one that can be blended into cosmetics, but preferably includes hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or its salts (sodium salt, etc.). For example, chondroitin sulfate or its salt can usually be purified and used from mammals or fish.

The sphingolipid may be any one that can be blended into cosmetics, but preferred examples include ceramide, phytosphingosine, and sphingolipid. Sphingolipids can usually be purified by conventional methods from mammals, fish, shellfish, yeast, or plants, or obtained by chemical synthesis.

The seaweed extract can be any one that can be mixed into cosmetics, but preferably includes brown algae extract, red algae extract, green algae extract, etc. Also, calrageenan, arginic acid, sodium alginate, etc. purified from these seaweed extracts. Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purifying seaweed by a conventional method.

In addition to the above essential ingredients, the cosmetic of the present invention can be blended with other ingredients that are usually blended in cosmetics as needed. Other ingredients that may be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, disinfectants, antioxidants, plant extracts, pH adjusters, alcohol, colorants, fragrances, Examples include blood circulation promoters, cooling agents, antiperspirants, and purified water. Examples of oil and fat components include ester-based fats and oils, hydrocarbon-based fats and oils, silicone-based fats and oils, fluorine-based fats and oils, animal fats and oils, and vegetable fats and oils.

Ester-based oils include glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid. Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine. Diisopropyl acid, isoalkyl neopentanoate, tri(caprylic, capric acid)glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, pentaelislitol tetra2-ethylhexanoate , cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, isostear laurate. Reyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, cetoste 2-ethylhexanoate. Aryl, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanate, ethylene glycol dioleate, propylene glycol dicapric acid, di(capryl, capric acid) propylene glycol, propylene glycol dicaprylate, dicapric acid Neopentyl glycol prinate, neopentyl glycol dioctanate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostear octanoate. Reyl, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleic acid ester, polyglycerol isostearic acid ester. , triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, malic acid. Diisostearyl acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate. , hydroxycholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 12-stealoyl hydroxystearate isocetyl, 12-steallo. Ester systems such as stearyl monohydroxystearate and isostearyl 12-stealoylhydroxystearate can be mentioned.

Examples of hydrocarbon oils include squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax, and petroleum jelly.

Silicone oils include polymethyl silicone, methylphenyl silicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane and methylcetyloxysiloxane copolymer, dimethylsiloxane and methylstealoxysiloxane copolymer, and alkyl. Modified silicone oil, amino-modified silicone oil, etc. can be mentioned.

Examples of fluorine-based fats and oils include perfluoropolyether.

Animal or plant oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, birdflower oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, and grape seed oil. , cottonseed oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, walnut colostrum oil, marker damia nut oil, meadow oil, egg yolk oil, beef tallow, horse oil, mink oil, orange rape oil, jojoba oil. , animal or plant oils such as candelier wax, carnaba wax, liquid lanolin, and hydrogenated castor oil.

Moisturizers include water-soluble low-molecular-weight moisturizers, oil-soluble molecular moisturizers, water-soluble polymers, and fat-soluble polymers.

Water-soluble low-molecular-weight moisturizers include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (degree of polymerization n = 2 or more), and polypropylene. Examples include glycol (degree of polymerization n = 2 or more), polyglycerin B (degree of polymerization n = 2 or more), lactic acid, lactate, etc.

Examples of fat-soluble low-molecular-weight moisturizers include cholesterol and cholesterol esters.

Water-soluble polymers include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water-soluble chitin, chitosan, and dextrin. You can.

Examples of oil-soluble polymers include polyvinylpyrrolidone and eicosene copolymers, polyvinylpyrrolidone and hexadecene copolymers, nitrocellulose, dextrin fatty acid ester, and polymer silicone. Examples of emollients include long-chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

Examples of surfactants include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE. Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE and POP (polyoxyethylene and polyoxypropylene) copolymer, POE and POP alkyl ether, polyether modified silicone, lauric acid. Examples include alkanolamide, alkylamine oxide, and hydrogenated soybean phospholipid.

Anionic surfactants include fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkyl amide sulfate, alkyl phosphate, POE alkyl phosphate industry, alkyl amide. Phosphate, alkyloyl alkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkyl sulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. there is.

Cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, and chloride. Examples include benzalkonium, diethylaminoethylamide stearate, dimethylaminopropyl stearate, and quaternary ammonium salts of lanolin derivatives. Amphoteric surfactants include carboxy beta type, amide beta type, sulfo beta type, hydroxy sulfo beta type, amide sulfo betaine type, phosphobetain type, aminocarboxylate type, imidazoline derivative type, and amidamine type. Amphoteric surfactants, etc. can be mentioned.

Organic and inorganic pigments include silicic acid, anhydrous silicic acid, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, and aluminum oxide. Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene and styrene copolymer, Examples include organic pigments such as silk powder, cellulose, CI pigment yellow, and CI pigment orange, and complex pigments of these inorganic pigments and organic pigments.

Examples of organic powder include metal soap such as calcium stearate; Alkyl phosphate metal salts such as sodium zinc cetilate, zinc laurylate, and calcium laurylate; Acyl amino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroyl glycine calcium; Amidesulfonic acid polyvalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylizine, N-alpha-paritoylolnithine, N-alpha-lauroylarginine, N-alpha-hydrogenated beef tallow fatty acid acylarginine, etc. -Acyl basic amino acid; N-acyl polypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Examples include polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene and styrene copolymers, and ethylene tetrafluoride.

UV absorbers include para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, and benzyl cinnamate. , paramethoxycinnamic acid-2-ethoxyethyl, octyl paramethoxycinnamic acid, mono-2-ethylhexane glyceryl diparamethoxycinnamic acid, isopropyl paramethoxycinnamic acid, diisopropyl and diisopropylcinnamic acid ester mixture, Urocanic acid, ethyl urocanic acid, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone disulfonate sodium, dihydroxybenzoate Phenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)- 1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc.

Disinfectants include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zincphyllithione, benzalkonium chloride, and photosensitive. Sub-No. 301, sodium mononitroguaiacol, undecirenic acid, etc. can be mentioned.

Antioxidants include butylhydroxyanisole, propyl gallate, and elisorbic acid.

Examples of pH adjusters include citric acid, sodium citrate, malic acid, sodium malate, fumal acid, sodium fumalate, succinic acid, sodium succinate, sodium hydroxide, and sodium monohydrogen phosphate.

Examples of alcohol include higher alcohols such as cetyl alcohol.

Additionally, the ingredients that may be added are not limited to this, and any of the ingredients can be blended within the range that does not impair the purpose and effect of the present invention.

The cosmetic of the present invention may take the form of a solution, emulsion, viscous mixture, etc.

The ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions as active ingredients, for example, conventional auxiliaries and carriers such as stabilizers, solubilizers, vitamins, pigments, and fragrances. Includes.

The cosmetic composition for skin whitening and skin aging prevention of the present invention can be manufactured in any formulation commonly manufactured in the art, for example, emulsion, cream, lotion, pack, foundation, lotion, beauty essence, hair cosmetics, etc. You can.

Specifically, the cosmetic composition of the present invention includes skin lotion, skin softener, skin toner, milk lotion, astringent, lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, foundation, essence, nutritional essence, It includes formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, hair lotions, hair tonics, hair essences, hair shampoos, hair rinses, hair treatments, body lotions, and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier ingredient. You can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. /May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide. Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative, or ethoxylated glycerol fatty acid ester can be used.

In one aspect, the present invention relates to a health functional food composition for skin improvement comprising fermented Japanese pine needles as an active ingredient, wherein the fermented Japanese pine needles contain ethanol extracts of Japanese pine nuts, Bacillus subtilis and Saccharomyces cereus. It relates to a health functional food composition for improving skin, which is complex fermented with Saccharomyces cerevisiae .

In addition to containing the active ingredient, the health functional food composition of the present invention may contain various flavoring agents or natural carbohydrates as additional ingredients like a typical food composition.

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose, etc.; Disaccharides such as maltose, sucrose, etc.; and polysaccharides, such as common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. The above-described flavoring agents include natural flavoring agents (thaumatin), stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.), and synthetic flavoring agents (saccharin, aspartame, etc.). The food composition of the present invention can be formulated in the same manner as the pharmaceutical composition below and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, meat, chocolate, foods, confectionery, pizza, ramen, other noodles, gum, candy, ice cream, alcoholic beverages, vitamin complexes, health supplements, etc. There is.

In addition, the food composition contains, in addition to the extract as the active ingredient, various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavors, colorants and thickening agents (cheese, chocolate, etc.), pectic acid, and salts thereof. , alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. In addition, the food composition of the present invention may contain pulp for the production of natural fruit juice and fruit juice drinks and vegetable drinks.

The health functional food composition of the present invention can be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, etc. In the present invention, 'health functional food composition' refers to a food manufactured and processed using raw materials or ingredients with functionality useful to the human body in accordance with Act No. 6727 on Health Functional Food, and has It means taking it for the purpose of controlling nutrients or obtaining useful health effects such as physiological effects. The health functional food of the present invention may contain common food additives, and its suitability as a food additive is determined according to the general rules and general test methods of the food additive code approved by the Ministry of Food and Drug Safety, unless otherwise specified. Determination is made according to relevant standards and standards. Items listed in the 'Food Additive Code' include, for example, chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; Natural additives such as dark pigment, licorice extract, crystalline cellulose, kaoliang pigment, and guar gum; Examples include mixed preparations such as sodium L-glutamate preparations, noodle additive alkaline preparations, preservative preparations, and tar coloring preparations. For example, health functional foods in the form of tablets are prepared by granulating a mixture of the active ingredient of the present invention with excipients, binders, disintegrants and other additives in a conventional manner, adding a lubricant, etc., and compression molding, or The mixture can be directly compression molded. Additionally, the health functional food in tablet form may contain a flavoring agent, etc., if necessary. Among capsule-type health functional foods, hard capsules can be manufactured by filling a regular hard capsule with a mixture of the active ingredient of the present invention mixed with additives such as excipients, and soft capsules can be prepared by mixing the active ingredient of the present invention with additives such as excipients. It can be manufactured by filling the mixture with a capsule base such as gelatin. The soft capsule may contain plasticizers such as glycerin or sorbitol, colorants, preservatives, etc., if necessary. The health functional food in the form of a ring can be prepared by molding a mixture of the active ingredient of the present invention and excipients, binders, disintegrants, etc. by a previously known method, and can be coated with white sugar or other coating agent as needed. Alternatively, the surface can be coated with substances such as starch or talc. Health functional food in the form of granules can be manufactured into granules by mixing a mixture of excipients, binders, disintegrants, etc. of the active ingredients of the present invention by a known method, and may contain flavoring agents, flavoring agents, etc., if necessary. You can.

In one aspect, the present invention relates to a feed composition for skin improvement comprising fermented Japanese pine needles as an active ingredient, wherein the fermented Japanese pine needles contain extracts of Japanese pine nuts, Bacillus subtilis and Saccharomyces cerevisiae ( It relates to a feed or feed additive composition for skin improvement, which is complex fermented with Saccharomyces cerevisiae .

The feed or feed additive composition of the present invention improves the skin of animals and has an antioxidant effect, so it can be expected to improve the health of the animal. The feed composition of the present invention can be manufactured in the form of fermented feed, compounded feed, pellet form, silage, etc. The fermented feed can be manufactured by fermenting organic matter by adding various microorganisms or enzymes other than the peptide of the present invention, and the compounded feed can be manufactured by mixing various types of general feed with the peptide of the present invention. Pellet-type feed can be manufactured by applying heat and pressure to the above-mentioned mixed feed, etc. in a pellet machine, and silage can be manufactured by fermenting the green feed with the microorganism according to the present invention. Wet fermented feed collects and transports organic matter such as food waste, mixes excipients for sterilization and moisture control in a certain ratio, and then ferments for more than 24 hours at a temperature suitable for fermentation to ensure that the moisture content is about 70%. It can be manufactured by adjusting it. Fermented dried feed can be manufactured by adjusting wet fermented feed to an additional drying process so that the moisture content is about 30% to 40%.

The feed composition of the present invention may further include ingredients added to conventional feed. Examples of ingredients added to such feed may include grain powder, meat powder, and beans. In the above, the grain powder may be one or more selected from rice flour, wheat flour, barley flour, and corn flour. In the above, the meat powder may be a meat powder obtained by pulverizing any one or more selected from chicken, beef, pork, and ostrich meat. In the above, the pulses may be one or more types selected from soybeans, kidney beans, peas, and black beans.

In addition to grain powder, meat powder, and pulses, which are the ingredients added to the conventional feed mentioned above, the feed composition of the present invention can add one or more selected from nutrients and minerals to increase the nutritional value of the feed, and feed quality. In order to prevent deterioration, it may contain one or more selected from anti-fungal agents, antioxidants, anti-coagulants, emulsifiers, and binders.

Hereinafter, the present invention will be described in more detail through examples and experimental examples. However, the following examples and experimental examples are provided as examples of the present invention, and if a detailed description of a technique or configuration well known to those skilled in the art is judged to unnecessarily obscure the gist of the present invention, the detailed description is omitted. It can be done, and the present invention is not limited thereby. The present invention is capable of various modifications and applications within the description of the claims described below and the scope of equivalents interpreted therefrom.

<Example 1> Preparation of fermented Wasong product

1-1. Manufacture of Wasson extract

100 g of dried Orostachys japonicus was mixed with 1 L of 90% (v/v) alcohol ethanol, extracted at 40°C under reduced pressure to 0.04 Mpa, extracted for 4 hours, and then freeze-dried to prepare an extract of Orostachys japonicus.

1-2. Manufacture of Wasong fermented product

Bacillus subtilis (ATCC33234) and Saccharomyces cerevisiae (ATCC4126) strains were each subcultured in standard medium, and then the initial number of bacteria was adjusted to 10 ⁸ and used in the experiment. For comparative experiments , Lactobacillus plantarum and Lactobacillus rhamnosus strains were each subcultured in standard media, and then the initial number of bacteria was adjusted to 10 ⁸ and used in the experiment.

3% (w/w) glucose was sterilized at 121°C for 15 minutes and then cooled to room temperature. 100 mg of the Japanese pine extract prepared in Example 1-1 and each strain were added at a concentration of 2 × 10 ⁹ CFU/mL at the weight ratio shown in Table 1, and then fermented at 37°C for 72 hours. After fermentation, it was sterilized at 121°C for 15 minutes, cooled to room temperature, and filtered. Afterwards, it was freeze-dried to produce fermented Wasong product.

Manufacturing example composition One Lactobacillus plantarum ( Lactobacillus plantarum ) fermented product 2 Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) fermented product 3 Bacillus subtilis ( Bacillus subtilis ) fermented product 4 Saccharomyces cerevisiae fermented product 5 Bacillus subtilis: Saccharomyces cerevisiae (1:2) Wasong fermented product 6 Bacillus subtilis: Saccharomyces cerevisiae (1:5) Wasong fermented product 7 Bacillus subtilis: Saccharomyces cerevisiae (1:10) Wasong fermented product 8 Wasong extract control group ascorbic acid

<Example 2> DPPH free radical scavenging activity (antioxidant) of fermented Wasong product

DPPH was dissolved in ethanol at a concentration of 0.2mM, 100μL of the DPPH solution and 100μL of each prepared sample were reacted for 20 minutes, and the absorbance was measured at 517nm with a microplate reader. DPPH free radical scavenging ability was calculated using the following equation. At this time, ascorbic acid was used as the positive control group.

[Equation 1]

DPPH inhibition(%) = [1-(Abs _sample /Abs _control )] × 100

As shown in Figure 1, Lactobacillus plantarum, Lactobacillus rhamnosus, Bacillus subtilis ( BS), and Saccharomyces cerevisiae ( SC) It was confirmed that the DPPH free radical scavenging ability of the Wasong fermented product obtained by complex fermentation of Bacillus subtilis and Saccharomyces cerevisiae at a weight ratio of 1:2 to 10 was significantly superior to that of the Wasong fermented product used individually.

<Example 3> ABTS free radical scavenging activity (antioxidant) of fermented Wasong product

ABTS solution was prepared by mixing 7mM ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) and 2.45mM potassium persulfate in a 1:1 ratio and storing in the dark for 16 hours. The ABTS solution was diluted in 50% (v/v) methanol and adjusted to an absorbance of 0.7 ± 0.02 at 745 nm. After reacting 180 μL of ABTS solution and 20 μL of each prepared sample for 10 minutes, the absorbance was measured at 745 nm with a microplate reader. ABTS free radical scavenging ability was calculated using the following equation. At this time, ascorbic acid was used as the positive control group.

[Equation 2]

ABTS inhibition(%) = [1-(Abs _sample /Abs _control )] × 100

As shown in Figure 2, Lactobacillus plantarum, Lactobacillus rhamnosus, Bacillus subtilis ( BS), and Saccharomyces cerevisiae ( SC) It was confirmed that the ABTS free radical scavenging ability of the Wasong fermented product obtained by complex fermentation of Bacillus subtilis and Saccharomyces cerevisiae at a weight ratio of 1:2 to 10 was significantly superior compared to the fermented product used individually.

<Example 4> FRAP assay (antioxidant) of fermented Japanese pine needles

Acetate buffer (300mM, pH3.6): TPTZ (10mM) sample was dissolved in HCl (40mM) and mixed with FeCl3.6H2O (20mM) in a ratio of 10:1:1 and stored at 37°C in the dark to prepare FRAP solution. 190ul of the FRAP solution and 10ul of each prepared sample were reacted at 37°C for 10 minutes, and then the absorbance was measured at 593nm using a microplate reader.

As shown in Figure 3, Lactobacillus plantarum, Lactobacillus rhamnosus, Bacillus subtilis ( BS), and Saccharomyces cerevisiae ( SC) Compared to the Wasong fermented product used individually, the Wasong fermented product co-fermented with Bacillus subtilis and Saccharomyces cerevisiae at a weight ratio of 1:2 to 10 showed high FRAP reducing power, especially at a weight ratio of 1:5. It was confirmed that the highest FRAP reducing power was shown in the Wasong fermented product complex-fermented with .

<Example 5> Measurement of antioxidant improvement index

HaCaT cells were distributed at 1 × 10 ⁶ cell/mL in a 6 well plate and cultured for 24 hours. Each well was treated with 10 ng/mL of TNF-α and 10 ng/mL of IFN-γ diluted in serum-free DMEM for 24 hours. The medium was removed and washed twice with DPBS. Cells were destroyed using Trizol, chloroform was added, and centrifuged. The supernatant of the clear layer was collected, an equal amount of isopropyl alcohol was added, and centrifuged. After obtaining the pellet, it was washed with 70% (v/v) ethanol aqueous solution.

The mRNA expression levels of antioxidant-related genes SOD-1, SOD-2, CAT, and NRF-2 were confirmed using RT-PCR using cDNA obtained from the RNA collected according to the above method using a cDNA synthesis kit. The primers used are listed in Table 2 below. PCR was performed for 30 cycles of 30 seconds at 95°C, 30 seconds at 56-60°C, and 30 seconds at 72°C. The product was confirmed through electrophoresis on a 1.2% agarose gel.

SOD-1 forward 5-TGCAGGGCATCATCAATTTCG-3 reverse 5-TGGGGCGATCCCAATTACACC-3 SOD-2 forward 5-TGCTGGAAGCCATCAAACGT-3 reverse 5-TAGTAAGCGTGCTCCCACAC-3 CAT forward 5-TAGCCTTCGACCCAAGCAAC-3 reverse 5-TCACATAGAATGCCCGCACC-3 NRF-2 forward 5-TGAGCAAGTTTGGGAGGAGC-3 reverse 5-TCTGCTGTGCTTTCAGGGTG-3 GAPDH forward 5-ATCATCAGCAATGCCTCCTG-3 reverse 5-CCTGCTTCACCACCTTCTTG-3

As shown in Figure 4, the antioxidant-related factor SOD-1 in Wasong fermented product obtained by complex fermentation of Bacillus subtilis (BS) and Saccharomyces cerevisiae ( SC) at a weight ratio of 1:5 , SOD-2, CAT, and NRF-2 were all found to have the highest increase in expression.

<Example 6> Measurement of skin barrier and moisturizing improvement index

HaCaT cells were distributed at 1 × 10 ⁶ cell/mL in a 6 well plate and cultured for 24 hours. Each well was treated with 10 ng/mL of TNF-α and 10 ng/mL of IFN-γ diluted in serum-free DMEM for 24 hours. The medium was removed and washed twice with DPBS. Cells were destroyed using Trizol, chloroform was added, and centrifuged. The supernatant of the clear layer was collected, an equal amount of isopropyl alcohol was added, and centrifuged. After obtaining the pellet, it was washed with 70% (v/v) ethanol aqueous solution.

The RNA collected according to the above method was used as cDNA obtained using a cDNA synthesis kit, and the mRNA of Aquaporin-3, Claudin-1, Filaggrin, and HAS-3 were used as measurement indicators to measure the effect of improving skin barrier and moisturization using RT-PCR. The expression level was confirmed. The primers used are listed in Table 3 below. PCR was performed for 30 cycles of 30 seconds at 95°C, 30 seconds at 56-60°C, and 30 seconds at 72°C. The product was confirmed through electrophoresis on a 1.2% agarose gel.

Aquaporin-3 forward 5-AGATGCTCCACATCCGCTAC-3 reverse 5-ATTCCAGCACCCCAAGAAGGC-3 Claudine-1 forward 5-GGCAGATCCAGTGCAAAGTC-3 reverse 5-CAAAGTAGGGCACCTCCCAG-3 Filaggrin forward 5-TCGGCAAATCCTGAAGAATCC-3 reverse 5-CTGTTTCTCTTGGGCTCTTGG-3 HAS-3 forward 5-ACCAGGAGGACCCTGACTAC-3 reverse 5-CCAGGACTCGAAGCATCTCG-3 GAPDH forward 5-ATCATCAGCAATGCCTCCTG-3 reverse 5-CCTGCTTCACCACCTTCTTG-3

As shown in Figures 5 to 8, the skin barrier and It was confirmed that the expression of Aquaporin-3, Claudin-1, Filaggrin, and HAS-3, all measurement indicators for measuring the moisturizing improvement effect, increased the highest.

As seen above, specific embodiments of the present invention have been described in detail, but those skilled in the art who understand the spirit of the present invention may add, change, delete, etc. other components within the scope of the same spirit, thereby creating other regressive inventions. However, other embodiments that are included within the scope of the present invention can be easily proposed. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive. The scope of the present invention is indicated by the scope of the claims described below rather than the detailed description above, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts are included in the scope of the present invention. It should be interpreted as

Claims (8)

It relates to a cosmetic composition for skin improvement containing fermented Wasson as an active ingredient,
The fermented Japanese pine tree is a cosmetic composition for improving skin, which is obtained by complex fermentation of Japanese pine extract with Bacillus subtilis and Saccharomyces cerevisiae .
According to paragraph 1,
A cosmetic composition for improving skin, wherein the Wasong extract is extracted with 60 to 100% (v/v) alcohol ethanol.
According to paragraph 1,
The fermented Wasong product is a cosmetic composition for improving skin, which is a complex fermentation of Bacillus subtilis and Saccharomyces cerevisiae at a weight ratio of 1:1 to 10.
According to paragraph 1,
The composition is a cosmetic composition for improving skin, which has an antioxidant effect, a skin barrier improvement effect, and a moisturizing effect.
It relates to a health functional food composition for skin improvement containing fermented Wasong as an active ingredient,
The fermented Japanese pine tree is a health functional food composition for improving skin, which is obtained by complex fermentation of Japanese pine extract with Bacillus subtilis and Saccharomyces cerevisiae .
According to clause 5,
The composition is a health functional food composition for improving skin, which has an antioxidant effect, a skin barrier improvement effect, and a moisturizing effect.
It relates to a feed composition for skin improvement containing fermented Wasong as an active ingredient,
The fermented Japanese pine tree is a feed composition for improving skin, which is obtained by complex fermentation of Japanese pine extract with Bacillus subtilis and Saccharomyces cerevisiae .
It relates to a feed additive composition for skin improvement containing fermented Japanese pine needles as an active ingredient,
The fermented Japanese pine tree is a feed additive composition for skin improvement, which is obtained by complex fermentation of the Japanese pine extract with Bacillus subtilis and Saccharomyces cerevisiae .