KR20230141961A - Antioxidant composition comprising ginger juice as an active ingredient - Google Patents

Abstract

The present invention relates to an antioxidant composition containing ginger juice as an active ingredient. The ginger juice of the present invention contains total polyphenols and total flavonoids, and as active ingredients, 4-gingerol and 6-gingerol. It was confirmed that it contained diol (6-gingediol) and 6-gingerol (6-gingerol). In addition, it was confirmed that it effectively scavenges DPPH radicals and reduces iron, and by confirming the total reducing power, it was confirmed that the antioxidant effect was excellent.

Description

Antioxidant composition comprising ginger juice as an active ingredient}

The present invention relates to an antioxidant composition containing ginger juice as an active ingredient.

In order for a living body to live, it not only needs to consume nutrients, but also breathe actively. The oxygen that enters through this breathing burns nutrients and releases energy, making brain activities including physical exercise possible. At this time, a small amount of active oxygen is released. Free radicals that are highly reactive with ROS are generated, and tissue damage caused by active oxygen and/or free radicals is caused by aging of the body or other internal and external factors. Oxidative stress is generally known to be an imbalance between cellular biochemical processes that produce and eliminate reactive oxygen species (ROS), and accumulation of ROS in neurons plays an important role in the pathogenesis of neurodegenerative disorders. . Active oxygen reacts with the SH group of proteins, causing loss of enzyme activity, forming cross-links, and oxidizing unsaturated fatty acids that make up DNA, RNA, enzymes, and cell membranes, forming peroxidized lipids. These peroxidized lipids are very toxic to the living body and cause diseases such as arteriosclerosis, blood circulation disorders, fatigue, diabetes, heart disease, liver failure, carcinogenesis, and cancer metastasis. Substances that prevent lipid peroxidation are called antioxidants.

Meanwhile, ginger ( Zingiber officinale Roscoe) is a plant native to Southeast Asia, the rhizome is fleshy, and it has a spicy taste and fragrant smell. In oriental medicine, the dried rhizome of ginger is used as a medicine. It treats chills, fever, headache, vomiting, sea water, and phlegm, and is also prescribed for abdominal pain, diarrhea, and stomachache caused by food poisoning. The pharmacological effects of ginger have been reported to include hyperlipidemia, anti-diabetic, and anti-cancer activities.

The purpose of the present invention is to provide an antioxidant food composition containing ginger ( Zingiber officinale ) juice as an active ingredient.

Another object of the present invention is to provide an antioxidant cosmetic composition containing ginger ( Zingiber officinale ) juice as an active ingredient.

Another object of the present invention is to provide an antioxidant feed composition containing ginger ( Zingiber officinale ) juice as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating diseases induced by oxidative stress, containing ginger ( Zingiber officinale ) juice as an active ingredient.

Another object of the present invention is to crush ginger ( Zingiber officinale ) and extract juice;

Filtering the squeezed ginger juice;

Drying the filtered ginger juice; and

Increasing 4-gingerol, 6-gingediol and 6-gingerol in ginger juice; To provide a method of increasing antioxidant activity in ginger juice containing.

In order to achieve the above object, the present invention provides an antioxidant food composition containing ginger ( Zingiber officinale ) juice as an active ingredient.

In addition, the present invention provides an antioxidant cosmetic composition containing ginger ( Zingiber officinale ) juice as an active ingredient.

In addition, the present invention provides an antioxidant feed composition containing ginger ( Zingiber officinale ) juice as an active ingredient.

Additionally, the present invention provides a pharmaceutical composition for preventing or treating diseases induced by oxidative stress, containing ginger ( Zingiber officinale ) juice as an active ingredient.

In addition, the present invention includes the steps of crushing and extracting ginger ( Zingiber officinale );

Filtering the squeezed ginger juice;

Drying the filtered ginger juice; and

It provides a method of increasing antioxidant activity in ginger juice, comprising: increasing 4-gingerol, 6-gingediol, and 6-gingerol in ginger juice.

The ginger juice of the present invention contains total polyphenols and total flavonoids, and includes 4-gingerol, 6-gingediol, and 6-gingerol as active ingredients. I confirmed that I was doing it. In addition, it was confirmed that it effectively scavenges DPPH radicals and reduces iron, and by confirming the total reducing power, it was confirmed that it has excellent antioxidant effect, so it can be usefully used in related industries.

Figure 1 shows the results of analysis of the active ingredients of ginger juice of the present invention by HPLC-MS.
Figure 2 is a diagram confirming the structures of three types of phenolic compounds contained in ginger juice of the present invention.
Figure 3 is a diagram confirming the DPPH radical scavenging ability of ginger juice of the present invention.
Figure 4 is a diagram measuring the reducing power of ginger juice of the present invention using the Ferric Reducing Power Assay.
Figure 5 is a diagram confirming the total antioxidant capacity of ginger juice of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the attached drawings. In the following description, detailed descriptions of techniques well known to those skilled in the art may be omitted. Additionally, when describing the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description may be omitted. In addition, the terminology used in this specification is a term used to appropriately express preferred embodiments of the present invention, and may vary depending on the intention of the user or operator or the customs of the field to which the present invention belongs.

Therefore, definitions of these terms should be made based on the content throughout this specification. Throughout the specification, when a part is said to “include” a certain element, this means that it may further include other elements rather than excluding other elements, unless specifically stated to the contrary.

The present invention provides an antioxidant food composition containing ginger ( Zingiber officinale ) juice as an active ingredient.

In addition to containing the active ingredient of the present invention, the food composition of the present invention may contain various flavoring agents or natural carbohydrates as additional ingredients like a normal food composition.

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose, etc.; Disaccharides such as maltose, sucrose, etc.; and polysaccharides, such as common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. The above-described flavoring agents include natural flavoring agents (thaumatin), stevia extracts (e.g. rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.). The food composition of the present invention can be formulated in the same way as the pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, meat, chocolate, foods, confectionery, pizza, ramen, other noodles, gum, candy, ice cream, alcoholic beverages, vitamin complexes, health supplements, etc. There is.

In addition to the extract as an active ingredient, the food composition contains various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavors, colorants and thickening agents (cheese, chocolate, etc.), pectic acid and its salts, It may contain alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. In addition, the food composition of the present invention may contain pulp for the production of natural fruit juice, fruit juice beverages, and vegetable beverages.

The functional food composition of the present invention can be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, etc. for antioxidant purposes. In the present invention, 'health functional food composition' refers to food manufactured and processed using raw materials or ingredients with functionality useful to the human body in accordance with Act No. 6727 on Health Functional Food, and refers to food that is related to the structure and function of the human body. It means taking it for the purpose of controlling nutrients or obtaining useful health effects such as physiological effects. The health functional food of the present invention may contain common food additives, and its suitability as a food additive is determined in accordance with the general provisions and general test methods of the food additive code approved by the Food and Drug Administration, unless otherwise specified. Judgment is made according to specifications and standards. Items listed in the 'Food Additive Code' include, for example, chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; Natural additives such as dark pigment, licorice extract, crystalline cellulose, kaoliang pigment, and guar gum; Examples include mixed preparations such as sodium L-glutamate preparations, noodle additive alkaline preparations, preservative preparations, and tar coloring preparations. For example, the health functional food in the form of a tablet is made by granulating a mixture of the active ingredient of the present invention with excipients, binders, disintegrants and other additives in a conventional manner, and then adding a lubricant and compression molding, or The mixture can be directly compression molded. In addition, the health functional food in the form of tablets may contain flavoring agents, etc., if necessary. Among capsule-type health functional foods, hard capsules can be manufactured by filling a regular hard capsule with a mixture of the active ingredient of the present invention mixed with additives such as excipients, and soft capsules can be prepared by mixing the active ingredient of the present invention with additives such as excipients. It can be manufactured by filling the mixture with a capsule base such as gelatin. The soft capsule may contain plasticizers such as glycerin or sorbitol, colorants, preservatives, etc., if necessary. The health functional food in the form of a pill can be prepared by molding a mixture of the active ingredient of the present invention and excipients, binders, disintegrants, etc., using a known method. If necessary, it can be coated with white sugar or other coating agent. Alternatively, the surface can be coated with substances such as starch or talc. Health functional food in the form of granules can be manufactured into granules by mixing a mixture of excipients, binders, disintegrants, etc. of the active ingredients of the present invention by a known method, and may contain flavoring agents, flavoring agents, etc., if necessary. You can.

According to one embodiment of the present invention, the ginger juice may have an increased total polyphenol content.

According to one embodiment of the present invention, the ginger juice may have an increased total flavonoid content.

According to one embodiment of the present invention, the ginger juice may have an increased content of 4-gingerol, 6-gingediol, or 6-gingerol.

According to one embodiment of the present invention, the ginger juice may scavenge DPPH radicals.

According to one embodiment of the present invention, the ginger juice may reduce peroxide.

According to one embodiment of the present invention, the ginger juice may have a total antioxidant capacity of 350 to 400 mg AAE/g.

In addition, the present invention provides an antioxidant cosmetic composition containing ginger ( Zingiber officinale ) juice as an active ingredient.

Cosmetic compositions containing the active ingredients of the present invention can be used in a variety of ways for antioxidant purposes. Products to which this composition can be added include, for example, cosmetics such as various creams, lotions, skins, essences, etc., as well as shampoos, rinses, cleansers, face washes, soaps, treatments, packs, and beauty essences.

The cosmetic of the present invention includes a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular weight peptides, high molecular weight polysaccharides, sphingolipids, and seaweed extracts.

Water-soluble vitamins may be any that can be mixed into cosmetics, but preferably include vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, and vitamin H. and their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbic acid-2-phosphate, etc.) are also water-soluble vitamins that can be used in the present invention. Included. Water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microbial cultures, enzymatic methods, or chemical synthesis.

Oil-soluble vitamins may be any that can be mixed into cosmetics, but preferably include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), etc. , their derivatives (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinic acid, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microbial cultures, enzymatic or chemical synthesis.

The polymer peptide may be any peptide that can be blended into cosmetics, but preferably includes collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. High molecular weight peptides can be purified and obtained by conventional methods such as purification from microbial culture media, enzymatic methods, or chemical synthesis, or they can usually be purified and used from natural products such as dermis of pigs or cows or silk fiber of silkworms.

The high molecular polysaccharide may be any one that can be blended into cosmetics, but preferably includes hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or its salts (sodium salt, etc.). For example, chondroitin sulfate or its salt can usually be purified and used from mammals or fish.

The sphingolipid may be any one that can be incorporated into cosmetics, but preferably includes ceramide, phytosphingosine, and sphingolipid. Sphingolipids can usually be purified by conventional methods from mammals, fish, shellfish, yeast, or plants, or obtained by chemical synthesis.

The seaweed extract may be any one that can be blended into cosmetics, but preferably includes brown algae extract, red algae extract, green algae extract, etc. Also, calrageenan, arginic acid, sodium alginate, etc. purified from these seaweed extracts. Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purifying seaweed by a conventional method.

In addition to the above-mentioned essential ingredients, the cosmetic of the present invention may contain other ingredients usually blended in cosmetics as needed. Other ingredients that may be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, disinfectants, antioxidants, plant extracts, pH adjusters, alcohol, colorants, fragrances, Examples include blood circulation promoters, cooling agents, antiperspirants, and purified water. Examples of oil and fat components include ester-based fats and oils, hydrocarbon-based fats and oils, silicone-based fats and oils, fluorine-based fats and oils, animal fats and oils, and vegetable fats and oils.

Ester-based oils include glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid. Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine. Diisopropyl acid, isoalkyl neopentanoate, tri(caprylic, capric acid)glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, pentaelislitol tetra2-ethylhexanoate , cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, isostear laurate. Reyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, cetoester 2-ethylhexanoate Aryl, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanate, ethylene glycol dioleate, propylene glycol dicapric acid, di(capryl, capric acid) propylene glycol, propylene glycol dicaprylate, dicapric acid Neopentyl glycol prinate, neopentyl glycol dioctanate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostear octanoate. Reyl, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleic acid ester, polyglycerol isostearic acid ester. , Triisocetyl citrate, Triisoalkyl citrate, Triisooctyl citrate, Lauryl lactate, Myristyl lactate, Cetyl lactate, Octyldecyl lactate, Triethyl citrate, Acetyltriethyl citrate, Acetyltributyl citrate, Trioctyl citrate, Mal. Diisostearyl acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate. , cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 12-stealoyl hydroxystearate isocetyl, 12-stealoyl Ester systems such as stearyl monohydroxystearate and isostearyl 12-stealoylhydroxystearate can be mentioned.

Hydrocarbon-based fats and oils include squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax, and petroleum jelly.

Silicone oils include polymethyl silicone, methylphenyl silicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane and methylcetyloxysiloxane copolymer, dimethylsiloxane and methylstealoxysiloxane copolymer, and alkyl. Modified silicone oil, amino-modified silicone oil, etc. can be mentioned.

Examples of fluorine-based fats and oils include perfluoropolyether.

Animal or plant oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, birdflower oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, and grape seed oil. , cottonseed oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, walnut colostrum oil, marker damia nut oil, meadow oil, egg yolk oil, beef tallow, horse oil, mink oil, orange rape oil, jojoba oil. , animal or plant oils such as candelier wax, carnaba wax, liquid lanolin, and hydrogenated castor oil.

Moisturizers include water-soluble low-molecular-weight moisturizers, oil-soluble molecular moisturizers, water-soluble polymers, and fat-soluble polymers.

Water-soluble low-molecular-weight moisturizers include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (degree of polymerization n = 2 or more), and polypropylene. Examples include glycol (degree of polymerization n = 2 or more), polyglycerin B (degree of polymerization n = 2 or more), lactic acid, lactate, etc.

Examples of fat-soluble low-molecular-weight moisturizers include cholesterol and cholesterol esters.

Water-soluble polymers include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. You can.

Examples of oil-soluble polymers include polyvinylpyrrolidone and eicosene copolymers, polyvinylpyrrolidone and hexadecene copolymers, nitrocellulose, dextrin fatty acid ester, and polymer silicone. Examples of emollients include long-chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

Examples of surfactants include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE. Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE and POP (polyoxyethylene and polyoxypropylene) copolymer, POE and POP alkyl ether, polyether modified silicone, lauric acid. Examples include alkanolamide, alkylamine oxide, and hydrogenated soybean phospholipid.

Anionic surfactants include fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkylnaphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkyl amide sulfate, alkyl phosphate, POE alkyl phosphate industry, alkyl amide. Phosphate, alkyloyl alkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkyl sulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. there is.

Cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, and cationic surfactant. Examples include benzalkonium, diethylaminoethylamide stearate, dimethylaminopropyl stearate, and quaternary ammonium salts of lanolin derivatives. Amphoteric surfactants include carboxy beta type, amide beta type, sulfo beta type, hydroxy sulfo beta type, amide sulfo betaine type, phosphobetain type, aminocarboxylate type, imidazoline derivative type, and amidamine type. Amphoteric surfactants, etc. can be mentioned.

Organic and inorganic pigments include silicic acid, anhydrous silicic acid, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, and aluminum oxide. Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenolic resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene and styrene copolymer, Examples include organic pigments such as silk powder, cellulose, CI pigment yellow, and CI pigment orange, and complex pigments of these inorganic pigments and organic pigments.

Examples of organic powder include metal soap such as calcium stearate; Alkyl phosphate metal salts such as sodium zinc cetilate, zinc laurylate, and calcium laurylate; Acyl amino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroyl glycine calcium; Amidesulfonic acid polyvalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylizine, N-alpha-paritoylolnithine, N-alpha-lauroylarginine, N-alpha-hydrogenated beef tallow fatty acid acylarginine, etc. -Acyl basic amino acid; N-acyl polypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Examples include polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene and styrene copolymers, and ethylene tetrafluoride.

UV absorbers include para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, and benzyl cinnamate. , paramethoxycinnamic acid-2-ethoxyethyl, octyl paramethoxycinnamic acid, mono-2-ethylhexane glyceryl diparamethoxycinnamic acid, isopropyl paramethoxycinnamic acid, diisopropyl and diisopropylcinnamic acid ester mixture, Urocanic acid, ethyl urocanic acid, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone disulfonate sodium, dihydroxybenzoate Phenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)- 1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc. are mentioned.

Disinfectants include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zincphyllithione, benzalkonium chloride, and photosensitive. Sub-No. 301, mononitroguaiacol sodium, undecirenic acid, etc. can be mentioned.

Antioxidants include butylhydroxyanisole, propyl gallate, and elisorbic acid.

Examples of pH adjusters include citric acid, sodium citrate, malic acid, sodium malate, fumal acid, sodium fumalate, succinic acid, sodium succinate, sodium hydroxide, and sodium monohydrogen phosphate.

Examples of alcohol include higher alcohols such as cetyl alcohol.

Additionally, the ingredients that may be added are not limited to this, and any of the above ingredients can be blended within the range that does not impair the purpose and effect of the present invention.

The cosmetic of the present invention may take the form of a solution, emulsion, viscous mixture, etc.

The ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions as active ingredients, for example, conventional auxiliaries and carriers such as stabilizers, solubilizers, vitamins, pigments, and fragrances. Includes.

The antioxidant cosmetic composition of the present invention can be prepared in any formulation commonly manufactured in the art, and examples include emulsions, creams, lotions, packs, foundations, lotions, beauty essences, hair cosmetics, etc.

Specifically, the cosmetic composition of the present invention includes skin lotion, skin softener, skin toner, milk lotion, astringent, lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, foundation, essence, nutritional essence, It includes formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, hair lotions, hair tonics, hair essences, hair shampoos, hair rinses, hair treatments, body lotions, and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier ingredient. You can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. /May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide. Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative, or ethoxylated glycerol fatty acid ester can be used.

In addition, the present invention provides an antioxidant feed composition containing ginger ( Zingiber officinale ) juice as an active ingredient.

The feed composition of the present invention has excellent antioxidant effects, so it can be expected to improve the health of animals, improve the weight gain and meat quality of livestock, and increase milk production and immunity. The feed composition of the present invention can be manufactured in the form of fermented feed, compounded feed, pellet form, silage, etc. The fermented feed can be manufactured by fermenting organic matter by adding various microorganisms or enzymes other than the peptide of the present invention, and the compounded feed can be manufactured by mixing various types of general feed with the peptide of the present invention. Pellet-type feed can be manufactured by applying heat and pressure to the above-mentioned mixed feed, etc. in a pellet machine, and silage can be manufactured by fermenting the green feed with the microorganism according to the present invention. Wet fermented feed collects and transports organic matter such as food waste, mixes excipients for sterilization and moisture control at a certain ratio, and then ferments for more than 24 hours at a temperature suitable for fermentation to ensure that the moisture content is about 70%. It can be manufactured by adjusting it. Fermented dried feed can be manufactured by adjusting wet fermented feed to an additional drying process so that the moisture content is about 30% to 40%.

The feed composition of the present invention may further include ingredients added to conventional feed. Examples of ingredients added to such feed may include grain powder, meat powder, and beans. In the above, the grain powder may be one or more selected from rice flour, wheat flour, barley flour, and corn flour. In the above, the meat powder may be a meat powder obtained by pulverizing any one or more selected from chicken, beef, pork, and ostrich meat. In the above, the pulses may be one or more types selected from soybeans, kidney beans, peas, and black beans.

In addition to grain powder, meat powder, and pulses, which are the ingredients added to the conventional feed mentioned above, the feed composition of the present invention can add one or more selected from nutrients and minerals to increase the nutritional value of the feed, and feed quality. To prevent deterioration, it may contain one or more selected from anti-fungal agents, antioxidants, anti-coagulants, emulsifiers, and binders.

Additionally, the present invention provides a pharmaceutical composition for preventing or treating diseases induced by oxidative stress, containing ginger ( Zingiber officinale ) juice as an active ingredient.

The pharmaceutical composition of the present invention may further include adjuvants in addition to the active ingredients. The auxiliary agent may be any one known in the art without limitation, but the effect may be increased by further including, for example, Freund's complete auxiliary agent or incomplete auxiliary agent.

The pharmaceutical composition according to the present invention can be prepared by incorporating the active ingredient into a pharmaceutically acceptable carrier. Here, pharmaceutically acceptable carriers include carriers, excipients, and diluents commonly used in the pharmaceutical field. Pharmaceutically acceptable carriers that can be used in the pharmaceutical composition of the present invention include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, Examples include calcium phosphate, calcium silicate, cellulose, methyl cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The pharmaceutical composition of the present invention can be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, or sterile injection solutions according to conventional methods. .

When formulated, it can be prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and such solid preparations contain the active ingredient plus at least one excipient, such as starch, calcium carbonate, sucrose, lactose, and gelatin. It can be prepared by mixing etc. Additionally, in addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral administration include suspensions, oral solutions, emulsions, and syrups. In addition to the commonly used diluents such as water and liquid paraffin, they contain various excipients such as wetting agents, sweeteners, fragrances, and preservatives. You can. Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, and suppositories. Non-aqueous solvents and suspensions include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. As a base for suppositories, witepsol, tween 61, cacao, laurel, glycerogelatin, etc. can be used.

The pharmaceutical composition according to the present invention can be administered to an individual through various routes. All modes of administration are contemplated, for example, by oral, intravenous, intramuscular, subcutaneous, or intraperitoneal injection.

The dosage of the pharmaceutical composition according to the present invention is selected taking into account the age, weight, gender, physical condition, etc. of the individual. It is obvious that the concentration of the active ingredient included in the pharmaceutical composition can be selected in various ways depending on the target, and is preferably included in the pharmaceutical composition at a concentration of 0.01 to 5,000 μg/ml. If the concentration is less than 0.01 ㎍/ml, pharmaceutical activity may not appear, and if it exceeds 5,000 ㎍/ml, it may be toxic to the human body.

According to one embodiment of the present invention, the diseases induced by oxidative stress include aging, chronic alcoholism, atherosclerosis, cancer, coronary heart disease, cataracts, diabetes, Down syndrome, hepatitis, ischemic or reperfusion damage, and rheumatic diseases. It may be a disease selected from the group consisting of arthritis, arthrosis, renal failure, inflammatory response, various neurodegenerative diseases, stroke, and myocardial infarction.

In addition, the present invention includes the steps of crushing and extracting ginger ( Zingiber officinale );

Filtering the squeezed ginger juice;

Drying the filtered ginger juice; and

Increasing 4-gingerol, 6-gingediol and 6-gingerol in ginger juice; Provides a method of increasing antioxidant activity in ginger juice containing.

Hereinafter, the present invention will be described in more detail by examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited to these examples.

<Preparation Example 1> Preparation of ginger juice

In order to obtain the ginger juice of the present invention, ginger ( Zingiber officinale Roscoe) was ground at room temperature, and the ground product was filtered to obtain ginger juice. The obtained ginger juice was placed in a drying oven and dried to obtain dried ginger juice (GJ).

<Example 1> Component analysis of ginger juice

<1-1> Total polyphenol content analysis

To analyze total polyphenols, 200 μl of the obtained ginger juice was mixed with 1 ml of Folin-Ciocalteu reagent (0.2N) and reacted for 5 minutes. Afterwards, 800 μl of aqueous sodium carbonate solution (7.5% w/v) was added and reacted for 1 hour. After completion of the reaction, absorbance was measured at 760 nm. To quantify the total phenol content, the absorbance of the blank was measured at 760 nm, and gallic acid (GA) was used as a standard material to obtain a standard curve for quantification. The total polyphenol content was expressed as mg GAE/g Extract using the content of gallic acid, a standard substance.

<1-2> Total flavonoid content analysis

To analyze total flavonoids, 200 μl of ginger juice at a concentration of 500 μg/ml, 1 ml of distilled water, and sodium nitrate (5% w/v) were mixed and reacted for 6 minutes. Afterwards, 120 μl of aluminum chloride (10% w/v) was added and reacted for 5 minutes. After completion of the reaction, 400 μl of NaOH (1M) was added to make it basic. Afterwards, the absorbance was measured at 430 nm. To quantify the total flavonoid content, the absorbance of the blank was measured at 430 nm, and quercetin (QE) was used as a standard material to obtain a standard curve for quantification. The total flavonoid content was expressed as mg QE/g Extract using the content of quercetin, a standard substance.

The results of total polyphenol and total flavoid content analysis are shown in Table 1 below.

Total polyphenols
(mg GAE/g Extract) Total flavonoids
(mg QE/g Extract) ginger juice 18.48 ± 1.14 7.26 ± 2.05

As a result, it was confirmed that 18.48 mg of total polyphenols were contained per 1 g of ginger juice, and 7.26 mg of total flavonoids were confirmed to be contained per 1 g of ginger juice.

<1-3> Qualitative and semi-quantitative analysis of ginger juice

The content of the compounds in ginger juice was analyzed through qualitative and semi-quantitative analysis of phenolic compounds in ginger juice. Specifically, qualitative analysis of phenolic compounds was measured using a high-performance liquid chromatography (HPLC) system coupled with a mass spectrometer (Waters Alliance 2695 system, Milford, MA, USA). HPLC analysis was performed using a reversed-phase C18 column (250 A reel (40:50:10 v/v/v) was used, and phenolic compounds were eluted with 88% Solvent A and 12% Solvent 2 for 20 minutes. The flow rate of the eluent was 20 μl, and elution was performed at 1 ml/min. The standard solution and extract of ginger juice were dissolved in methanol and filtered using a millipore membrane (0.45 μm).

As a result, as shown in Figure 1, three types of active ingredients in ginger juice were confirmed, and the contents of the three active ingredients are shown in Table 2 below.

As a result, 4-gingerol, 6-gingediol, and 6-gingerol were detected as active ingredients contained in ginger juice.

In addition, the structures of 4-gingerol, 6-gingediol, and 6-gingerol identified in Table 2 were confirmed and shown in Figure 2.

<Example 2> Confirmation of antioxidant effect of ginger juice

<2-1> Confirmation of DPPH radical scavenging ability

In order to confirm the antioxidant effect of the ginger juice of the present invention, the DPPH radical scavenging ability was confirmed. Specifically, ginger juice was prepared at concentrations of 0.025, 0.05, 0.1, 0.2, and 0.4 mg/ml, and then 200 μl was mixed with 1.8 ml of DPPH and reacted for 30 minutes. Afterwards, the absorbance of the reaction mixture was measured at 517 nm, and the DPPH radical scavenging ability was calculated using Equation 1 below. In the equation below, the Abs control used the absorbance of DPPH, and the Abs sample used the absorbance value measured after the reaction between DPPH and ginger juice. As a positive control, ascorbic acid (AA), known as a natural antioxidant, was used.

As a result, as shown in Figure 3, it was confirmed that ginger juice scavenges DPPH radicals in a concentration-dependent manner.

<2-2> Check iron reducing power

In order to confirm the antioxidant effect of the ginger juice of the present invention, the reducing power was confirmed. Specifically, we wanted to analyze iron reducing power. After preparing ginger juice at concentrations of 25, 50, 100, 200, and 400 mg/ml, 500 μl of ginger juice of each concentration was mixed with 1.25 ml of phosphate buffer (0.2M; pH 6.6) and 1.25 ml of ginger juice. It was mixed with K ₃ Fe(CN) ₆ (1% w/v) and reacted at 50°C for 20 minutes. After completion of the reaction, the mixture was cooled, and 1.25 ml of trichloroacetic acid (10% w/v) was added to terminate the reaction. Afterwards, the mixture _was centrifuged at 1107 Mixed. To measure reducing power, the absorbance of the mixture was measured at 700 nm relative to the blank.

As a result, as shown in Figure 4, it was confirmed that ginger juice reduces iron in a concentration-dependent manner.

<2-3> Check total antioxidant capacity (TAC)

We sought to confirm the total antioxidant power of the ginger juice of the present invention. Specifically, molybdenum (VI), which exists in the form of molybdate (MoO ₄ ^2- ), is reduced to molybdate Mo(V) MoO ²⁺ and forms a green complex of phosphate/Mo(V) at acidic pH. Total antioxidant power was measured based on this. 300 μl of ginger juice was mixed with 3 ml of reagent solution (0.6 M sulfuric acid, 28 mM sodium phosphate, 4 mM ammonium molybdate). Afterwards, it was reacted at 95°C for 90 minutes. After completion of the reaction, it was cooled, and the absorbance of the blank was measured at 695 nm. The total amount of antioxidants was expressed as mg equivalent ascorbic acid/mg dry weight (mg EAA/g DW).

As a result, as shown in Figure 5, it was confirmed that the total antioxidant power of ginger juice was 348.70 mg AAE/g Extract, confirming the total antioxidant power of ginger juice.

Therefore, the ginger juice of the present invention contains total polyphenols and total flavonoids, and as active ingredients, 4-gingerol, 6-gingediol, and 6-gingerol. It was confirmed that it contains. In addition, it was confirmed that it effectively scavenges DPPH radicals and reduces iron, and by confirming the total reducing power, it was confirmed that the antioxidant effect was excellent.

Claims (12)

An antioxidant food composition containing ginger ( Zingiber officinale ) juice as an active ingredient. According to clause 1,
The ginger juice is a food composition with an increased total polyphenol content. According to clause 1,
The ginger juice is a food composition with an increased total flavonoid content. According to clause 1,
The ginger juice is a food composition with an increased content of 4-gingerol, 6-gingediol, or 6-gingerol. According to clause 1,
The ginger juice is a food composition that scavenges DPPH radicals. According to clause 1,
The ginger juice is a food composition that reduces peroxide. According to clause 1,
A food composition wherein the ginger juice has a total antioxidant capacity of 350 to 400 mg AAE/g. An antioxidant cosmetic composition containing ginger ( Zingiber officinale ) juice as an active ingredient. Antioxidant feed composition containing ginger ( Zingiber officinale ) juice as an active ingredient. A pharmaceutical composition for preventing or treating diseases induced by oxidative stress, comprising ginger ( Zingiber officinale ) juice as an active ingredient. According to clause 10,
Diseases induced by the oxidative stress include aging, chronic alcoholism, atherosclerosis, cancer, coronary heart disease, cataracts, diabetes, Down syndrome, hepatitis, ischemic or reperfusion damage, rheumatoid arthritis, arthritis, renal failure, inflammatory reactions, A composition that is a disease selected from the group consisting of various neurodegenerative diseases, strokes, and myocardial infarction. Grinding and extracting ginger ( Zingiber officinale );
Filtering the squeezed ginger juice;
Drying the filtered ginger juice; and
Increasing 4-gingerol, 6-gingediol and 6-gingerol in ginger juice; Method for increasing antioxidant activity in ginger juice containing.