KR102659125B1 - Cosmetic composition comprising probiotics cultivatied using dogo hot spring water - Google Patents

Abstract

The present invention relates to a culture containing a strain of the genus Lactobacillus cultured in a medium containing Dogo Hot Spring water; and a culture containing a strain of the genus Bifidobacterium cultured in a medium containing Dogo Hot Springs water as an active ingredient. The cosmetic composition of the present invention promotes the production of MMP-1 protein. It inhibits and promotes collagen production, and has the ability to inhibit tyrosinase and melanin production, making it excellent for skin whitening and wrinkle improvement.

Description

Cosmetic composition containing probiotics cultured using Dogo hot spring water {COSMETIC COMPOSITION COMPRISING PROBIOTICS CULTIVATIED USING DOGO HOT SPRING WATER}

The present invention relates to a cosmetic composition containing probiotics cultured using Dogo Hot Spring water, and more specifically, to a cosmetic composition containing probiotics cultured in a medium containing Dogo Hot Spring water and herbal medicine.

The skin is the largest organ in the body and is always in direct contact with the external environment, acting as a protective layer that protects the living body from various stimuli or dry environments. Compared to other organs, the creation and extinction of new cells occurs more actively. In addition, it protects the body from physical abrasions, prevents moisture loss inside the body, protects from ultraviolet rays generated by the sun, and plays a very important role in regulating body temperature.

Problems such as skin contamination, dryness, skin troubles, and skin immune system abnormalities occur due to various physical factors, external environmental factors, infections, etc. In particular, with modern rapid industrial development, pollution problems such as air pollution and water pollution have become more serious, and the strong heating and cooling of living spaces due to the improvement of residential environments has caused skin to be exposed to external environmental factors such as skin roughness, dryness, and aging. is greatly influenced by Human skin gradually becomes thinner, wrinkles increase, elasticity decreases, and spots and freckles increase due to exposure to ultraviolet rays due to intrinsic aging and photoaging. In particular, intrinsic aging occurs when the biosynthesis of biological proteins decreases as hormone secretion decreases or the function and activity of immune cells decreases, and this progresses rapidly due to the increase in reactive oxygen species.

It is known that humans continuously generate reactive oxygen species during the metabolic process, which acts as a direct or indirect cause of aging. Various antioxidants are used in cosmetics to prevent skin aging. Representative synthetic antioxidants include BHT (butylated hydroxytoluene), BHA (butylated hydroxyanisole), and tertiary butylhydroquinone (TBHQ). In general, synthetic antioxidants are widely used due to their excellent effectiveness and low price, but their use is restricted as they are known to cause not only self-toxicity but also hepatomegaly, fatty acid transformation, and cancer upon oral or skin contact.

Niacinamide and arbutin, which are used as whitening functional cosmetic ingredients, and retinol, which is used as a wrinkle-improving functional cosmetic ingredient, have problems with the whitening effect being reduced due to limitations in the mixing amount due to destruction of ingredients by oxidation and skin irritation. Recently, as various cosmetic compositions using natural substances have appeared on the market, consumers have become more interested in products using natural substances rather than existing products made of chemical substances. Accordingly, cosmetic compositions using various natural substances are being manufactured.

In addition, the wrinkle-improving effect of cosmetics is based on the effect of dermal layer improvement by various active ingredients in addition to the temporary effect of moisturizing by moisturizing factors, and the growth of retinol, vitamin C, and EGF, which are raw materials for representative wrinkle-improving cosmetics. Factor ingredients are well known to show anti-wrinkle efficacy by increasing collagen formation from human dermal fibroblasts. In order to prevent this phenomenon and maintain healthier and more beautiful skin, bioactive substances obtained from various known animals, plants, and microorganisms are added to cosmetics to maintain the skin's inherent functions and activate skin cells. Efforts have been made to effectively suppress aging.

In the field of cosmetics, research and development on functional cosmetics with whitening and wrinkle improvement effects are actively underway, and in particular, research using natural substances to avoid toxicity or irritation to the skin continues.

Korean Patent Publication No. 10-2021-0064818 Korean Patent No. 10-1896942

The purpose of the present invention is to provide a cosmetic composition that has a skin whitening or wrinkle improvement effect containing a probiotic culture cultured using Dogo hot spring water and herbal medicine extract as an active ingredient.

According to one aspect of the invention,

A culture containing a strain of the genus Lactobacillus cultured in a medium containing Dogo Hot Spring water; and

A cosmetic composition containing as an active ingredient a culture containing a strain of the genus Bifidobacterium cultured in a medium containing Dogo hot spring water is provided.

The medium containing the Dogo hot spring water may further include an herbal medicine extract including one or more selected from the group consisting of Mokdan bark extract, Gyejo axis extract, and Yeongyo extract.

The herbal medicine extract may be extracted with water, alcohol with 1 to 4 carbon atoms, or a mixed solvent thereof.

The medium containing the Dogo Hot Spring water may contain 10 to 40% (v/v) of the Dogo Hot Spring water.

The medium containing the Dogo hot spring water contains 1 to 5% (v/v) of the herbal medicine extract, and the herbal medicine extract may have a solid content of 10 to 20% (w/v).

The Dogo hot spring water contains 55 to 65 mg/L of calcium (Ca), 25 to 35 mg/L of sodium (Na), 10 to 20 mg/L of silicon (Si), 0.001 to 0.05 mg/L of lithium (Li), and strontium ( Sr) may contain 1.0 to 2.0 mg/L.

The Lactobacillus genus strains include Lactobacillus plantarum , Lactobacillus reuteri, Lactobacillus harbinensis , Lactobacillus casei, and Lactobacillus sakei ( Lactobacillus casei ). Among Lactobacillus sakei , Lactobacillus brevis, Lactobacillus pentosus, Lactobacillus fermentum , Lactobacillus acidophilus and Lactobacillus rhamnosus It can be any one selected.

The Bifidobacterium genus strains include Bifidobacterium longum , Bifidobacterium breve, Bifidobacterium lactis , and Bifidobacterium bifidum. It may be any one selected from ( Bifidobacterium bifidum ).

The cosmetic composition may be used for skin whitening or wrinkle improvement.

The cosmetic composition containing probiotics cultured using Dogo hot spring water of the present invention inhibits the production of MMP-1 protein, promotes collagen production, and has the ability to inhibit tyrosinase and melanin production, so it has excellent skin whitening and wrinkle improvement effects.

Since the present invention can be modified in various ways and can have various embodiments, specific embodiments will be illustrated in the drawings and described in detail in the detailed description. However, this is not intended to limit the present invention to specific embodiments, and should be understood to include all transformations, equivalents, and substitutes included in the spirit and technical scope of the present invention. In describing the present invention, if it is determined that a detailed description of related known technologies may obscure the gist of the present invention, the detailed description will be omitted.

Hereinafter, a cosmetic composition containing probiotics cultured using Dogo hot spring water of the present invention will be described.

The cosmetic composition of the present invention is a culture containing a strain of the genus Lactobacillus cultured in a medium containing Dogo hot spring water; And a culture containing a strain of the genus Bifidobacterium cultured in a medium containing Dogo Hot Spring water; characterized in that it contains as an active ingredient. The strain cultured in the culture is characterized in that it is used as an active ingredient in cosmetics without being removed.

Preferably, the medium containing the Dogo hot spring water may further include an herbal medicine extract including one or more selected from the group consisting of the Danbar bark extract, the extract from the axillary rhododendron, and the extract from the axillary rhododendron, and more preferably the extract of the herb extract, the extract of the axillary rhododendron, and Three types of complex herbal medicines including lotus leaf extract can be used. If any one of the three types is excluded, the wrinkle improvement or whitening effect may be significantly reduced compared to using the three types of combined herbal medicine.

The herbal medicine extract including the above-mentioned Mokdanpi, Gyejochuk, and Yeongyo may be extracted with water, alcohol with 1-4 carbon atoms, or a mixed solvent thereof, and preferably may be a hot water extract.

Mokdanpi is a medicinal herb made from the root bark of the peony (Paeonia suffruticosa Andrews) of the peony family and is used in the same way in China and Japan as in Korea. Mokdanpi is used for menstrual irregularities due to blood heat, menstrual pain, bruises, hematemesis, nosebleeds, spots, tingling bones due to heat, increased blood pressure, removal of stagnant blood, bruises, anti-inflammatory analgesics, boil treatment, early stages of appendicitis, etc. It is known to relieve chest tightness. It has been reported that its pharmacological effects include analgesic, sedative, antipyretic, anticonvulsant, anti-inflammatory, antithrombotic, antiallergic, suppressing gastric juice secretion, uterine mucosal congestion, and antibacterial effect. The appearance is a tubular piece of bark, the outer surface is dark brown or purple-brown, and there are long, horizontal, oval-shaped lateral root marks and vertical wrinkles. The inner surface is light gray-brown or dark purple and flat, and the curved surface is rough. White crystals are sometimes attached to the inner surface and the bent surface.

The gradation axis is the Manshurian fullmoon maple of the maple family, and its scientific name is Acer pseudo-sieboldianum (Paxton) Komarov. Deciduous broad-leaved small tree, about 8m tall, leaves are 7-10cm long, divided into 9-10 branches, hairs grow densely on the back and branches of young leaves, flowers are male and female, magenta in May, calyxes are There are 5-6 hairs and four petals. The bark part of the herbal medicine material is used, and the Gyejo axis extract of the present invention also uses the bark part.

Yeongyo refers to the dried fruit of Forsythia viridissima Lindley or Forsythia suspensa Vahl of the ash tree family. Yeongyo gets its name from the fact that the fruits form chambers similar to lotuses and protrude long from among various grasses. It has a unique smell, the taste is bitter, and the energy is slightly cold. It is known to have medicinal properties such as reducing fever, detoxifying, eliminating wind fever, and having the effect of relieving swollen boils or wounds or loosening knots or lumps. Antibacterial, anti-inflammatory, blood pressure lowering, hemostatic, liver healing, antipyretic, antiemetic, and diuretic effects have been reported as pharmacological effects. Main chemical components include forsythol, sterol compounds, saponin, and oleanolic acid.

The medium containing the Dogo Hot Spring water preferably contains 10 to 40% (v/v) of the Dogo Hot Spring water, and more preferably contains 25 to 35% (v/v). If it is less than 10% (v/v), the wrinkle improvement or whitening effect may be reduced, and if it is more than 40% (v/v), culturing probiotics may be difficult.

In addition, the medium containing the Dogo hot spring water preferably contains 1 to 5% (v/v) of the herbal medicine extract, and the herbal medicine extract preferably has a solid content of 10 to 20% (w/v), more preferably Contains 2 to 4% (v/v) of the herbal medicine extract, and the herbal medicine extract may have a solid content of 12 to 17% (w/v). If the herbal medicine extract is included in less than 1% (v/v), the wrinkle improvement or whitening effect may be reduced, and if it is more than 5% (v/v), skin toxicity may occur or probiotics may not be properly cultured. It may not be possible.

The Lactobacillus genus strains include Lactobacillus plantarum , Lactobacillus reuteri, Lactobacillus harbinensis , Lactobacillus casei, and Lactobacillus sakei ( Lactobacillus casei ). Among Lactobacillus sakei , Lactobacillus brevis, Lactobacillus pentosus, Lactobacillus fermentum , Lactobacillus acidophilus and Lactobacillus rhamnosus Any selected one is preferred, and more preferably Lactobacillus plantarum.

The Lactobacillus plantarum is a bacteria that produces useful metabolites for the human body and is safe enough to be used as a food raw material. The Lactobacillus Plantarum culture extract of the present invention is obtained by culturing the Lactobacillus Plantarum and disrupting the cells with a homogenizer, and contains metabolic components, cytoplasm, cell wall components, polysaccharides, etc. of Lactobacillus Plantarum. It is a fermented lysate.

In addition, the Bifidobacterium genus strains include Bifidobacterium longum , Bifidobacterium breve, Bifidobacterium lactis , and Bifidobacterium. Any one selected from Bifidobacterium bifidum is preferable, and more preferably, it may be Bifidobacterium longum .

Bifidobacterium longum is a Gram-positive lactic acid bacterium that exists in the human gastrointestinal tract. It has been isolated from the human body and proven to be safe, and is also known as a beneficial bacteria that improves the intestinal environment.

The Dogo hot spring water contains 55 to 65 mg/L of calcium (Ca), 25 to 35 mg/L of sodium (Na), 10 to 20 mg/L of silicon (Si), 0.001 to 0.05 mg/L of lithium (Li), and strontium ( Sr) 1.0 to 2.0 mg/L.

The cosmetic composition of the present invention is used for skin whitening or wrinkle improvement.

As used herein, the term “extract” includes not only crude extracts obtained by processing an extraction solvent, but also processed products of the extract. For example, the extract can be prepared in powder form by additional processes such as reduced pressure distillation and freeze-drying or spray drying.

In addition, the herbal medicine extract including Mokdanpi, Gyejochuk, and Yeongyo of the present invention has a meaning that includes formulated processed products, such as herbal medicine powder, in a broad sense. Although experiments were conducted with herbal medicine in the present invention, those skilled in the art would be able to predict that the desired effect can be achieved even in the form of a processed herbal medicine.

Meanwhile, in this specification, the term “including as an active ingredient” means containing a sufficient amount to achieve the efficacy or activity of the ingredient containing the probiotic culture. As an example, the probiotic culture containing the probiotic is used at a concentration of 10 to 1500 ㎍/㎖, preferably 100 to 1000 ㎍/㎖. Since the probiotic culture is a natural product and has no side effects on the human body even when applied to the skin in excessive amounts, the upper quantitative limit of the probiotic culture included in the composition of the present invention can be selected within an appropriate range by a person skilled in the art.

The term "skin whitening" used in the present invention refers to whitening the skin. A person's skin color is determined by the concentration and distribution of melanin inside the skin. In addition to genetic factors, UV rays from the sun, fatigue, stress, etc. It is also influenced by environmental or physiological conditions. Melanin is synthesized in melanocytes in the epidermal layer of the skin. In melanosomes, an organelle within melanocytes, an enzyme called tyrosinase acts on tyrosine, a type of amino acid, to produce DOPA and dopaquinone. ) and then produced through a non-enzymatic oxidation reaction. If this synthesis of melanin occurs excessively within the skin, it darkens the skin tone and causes spots, freckles, etc. Therefore, by inhibiting the synthesis of melanin pigment by inhibiting the activity of tyrosinase in the skin, not only can skin whitening be achieved by brightening the skin tone, but also skin hyperpigmentation such as spots and freckles caused by ultraviolet rays, hormones, and genetic causes. It can improve calmness.

In addition, in the present invention, the term "wrinkle" refers to fine lines that occur as the skin ages, and may be caused by genes, a decrease in collagen present in the skin dermis, external environment, etc. In the present invention, “wrinkle improvement” refers to suppressing or inhibiting the formation of wrinkles on the skin, or alleviating wrinkles that have already formed.

The main functions of skin fibroblasts include the regeneration of damaged skin tissue through extracellular matrix synthesis and proliferation, and endogenous aging factors such as extrinsic aging factors such as photoaging, accumulation of damage caused by free oxygen, and cell aging due to telomere disruption. They decrease the physiological activity of skin fibroblasts within the dermal layer. Type I collagen, which accounts for more than 95% of the skin's extracellular matrix and plays a very important part in the physiological activity of fibroblasts through interaction and signal exchange with cell adhesion molecules and cytoskeleton When the synthesis of collagen decreases, the amount of collagen in the skin tissue decreases, which leads to a decrease in surface tension, which not only reduces skin elasticity and forms skin wrinkles, but also reduces physiologically active functions, including cell proliferation and regeneration functions. It becomes the cause of a vicious cycle.

Therefore, collagen in skin fibroblasts can be considered to be directly related to skin regeneration, skin elasticity, skin wrinkle formation, and tissue repair or regeneration when skin is damaged. In other words, when the synthesis of collagen or procollagen in skin fibroblasts is promoted or the synthesis of matrix metallo-proteinase (hereinafter referred to as 'MMP'), which decomposes collagen, is inhibited, skin elasticity is improved, skin regeneration, Improving skin wrinkles, wound healing, repair and regeneration of damaged skin tissue; and skin aging prevention effects.

The cosmetic composition of the present invention is characterized by increasing the expression of type I procollagen protein and suppressing the expression of MMP-1 protein. Specifically, the cosmetic composition of the present invention inhibits the expression of matrix metallo-proteinase-1, which promotes collagen decomposition, while increasing the production of type I procollagen, thereby increasing skin elasticity and skin regeneration. , improving skin wrinkles, wound healing, repair and regeneration of damaged skin tissue; and may have effects such as preventing skin aging. In other words, it can have the effect of preventing or improving skin wrinkles.

In addition, the cosmetic composition of the present invention may further include water-soluble vitamins, fat-soluble vitamins, high molecular weight peptides, high molecular weight polysaccharides, sphingo lipids, and seaweed extract.

The water-soluble vitamins may be any that can be mixed into cosmetics, but are preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, and vitamin H. and the like, and their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbic acid-2-phosphate, etc.) can also be used in the present invention. Included in water-soluble vitamins. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microbial cultures, enzyme methods, or chemical synthesis.

The fat-soluble vitamins can be any one as long as they can be mixed in cosmetics, but preferably include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E, etc., and their derivatives (ascorbin palmitate, ascorbic acid stearate) Bean, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinic acid (vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) are also included in the fat-soluble vitamins used in the present invention. do.

The polymer peptide may be any one as long as it can be mixed into cosmetics, but preferably includes collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymer peptide can be purified and obtained by conventional methods such as purification of microbial culture broth, enzyme method, or chemical synthesis, or can be usually purified and used from natural products such as dermis of pigs, cows, etc., and silk fiber of silkworms.

The polymer polysaccharide can be anything as long as it can be mixed in cosmetics, but preferably includes hydroxyethyl cellulose, xanthan gum, chondroitin sulfate or its salts (sodium salt, etc.). For example, chondroitin sulfate or its salt can usually be purified and used from mammals or fish.

The sphingolipid may be anything that can be mixed into cosmetics, but preferably includes ceramide, phytosphingosine, and sphingosaccharide lipid. The sphingolipids can usually be purified by conventional methods from mammals, fish, shellfish, yeast, or plants, or obtained by chemical synthesis.

The seaweed extract can be anything as long as it can be mixed in cosmetics, but preferably includes brown algae extract, red algae extract, green algae extract, etc., and also calrageenan, arginic acid, and arginic acid purified from these seaweed extracts. Sodium, potassium alginate, etc. are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purifying seaweed by a conventional method.

In addition to the above-mentioned essential ingredients, the cosmetic composition of the present invention may contain other ingredients normally blended in cosmetics as needed.

Other ingredients that may be added include oils and fats, moisturizers, emollients, ultraviolet absorbers, pH adjusters, fragrances, blood circulation promoters, cooling agents, antiperspirants, and purified water.

The oil and fat components include ester-based fats, hydrocarbon-based fats, silicone-based fats and oils, fluorine-based fats and oils, animal fats and vegetable oils, and the like.

The ester-based oils include glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, Butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, Diisopropyl adipate, isoalkyl neopentanoate, tri(caprylic, capric acid)glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, pentaeli tetra2-ethylhexanoate Slitol, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, lauric acid Sostearyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, 2-ethylhexane acid wash. Tostearyl, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanate, ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl dioctanoate. Glycol, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyl neodecanoate. Decyl, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleic acid ester, polyglycerol isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, Triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, hydroxystearic acid 2- Ethylhexyl, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, hydroxycholesteryl stearate, cholesteryl oleate. Steryl, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, isocetyl 12-stealoyl hydroxystearate, 12-stealoyl hydroxystearate, 12-stealo Ester systems, such as isostearyl hydroxystearate, etc. are mentioned.

Examples of the hydrocarbon oil include squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax, and petroleum jelly.

The silicone oils include polymethyl silicone, methylphenyl silicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane methylcetyloxysiloxane copolymer, dimethylsiloxane methylstealoxysiloxane copolymer, alkyl Modified silicone oil, amino-modified silicone oil, etc. can be mentioned.

Examples of the fluorine-based oil include perfluoropolyether.

The animal or plant oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, cottonseed oil, palm oil, Cuckoo nut oil, wheat germ oil, rice germ oil, shea butter, moonshine colostrum, marker damia nut oil, egg yolk oil, beef tallow, horse oil, mink oil, orange rape oil, jojoba oil, candelier wax, carnaba wax, liquid ranol. and animal or plant oils such as hydrogenated castor oil.

Examples of the moisturizing agent include water-soluble low-molecular-weight moisturizers, oil-soluble molecular moisturizers, water-soluble polymers, and oil-soluble polymers.

The water-soluble low-molecular-weight moisturizers include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (degree of polymerization n = 2 or more), Examples include polypropylene glycol (degree of polymerization n = 2 or more), polyglycerin B (degree of polymerization n = 2 or more), lactic acid, and lactate salts.

Examples of the fat-soluble low-molecular-weight moisturizer include cholesterol and cholesterol ester.

The water-soluble polymers include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. can be mentioned.

Examples of the oil-soluble polymer include polyvinylpyrrolidone eicosene copolymer, polyvinylpyrrolidone hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollient agent include long-chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

The ultraviolet absorbers include para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, Benzyl cinnamic acid, paramethoxycinnamic acid-2-ethoxyethyl, octyl paramethoxycinnamic acid, mono-2-ethylhexane glyceryl diparamethoxycinnamic acid, isopropyl paramethoxycinnamic acid, diisopropylㆍdiisopropylcinnamic acid ester mixture , Urocanic acid, ethyl urocanic acid, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone disulfonate sodium, dihydroxy Benzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy) -1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc. are mentioned.

Examples of the pH adjusting agent include citric acid, sodium citrate, malic acid, sodium malate, fumal acid, sodium fumalate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, and the like.

Examples of the pH adjusting agent include citric acid, sodium citrate, malic acid, sodium malate, fumal acid, sodium fumalate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, and the like.

Cosmetics manufactured containing the cosmetic composition of the present invention may take the form of a solution, emulsion, viscous mixture, etc.

In addition, the ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the ingredients as active ingredients, for example, conventional auxiliaries such as stabilizers, pigments, and natural fragrances, and It may further contain a carrier.

The cosmetic composition of the present invention can be used in a variety of ways, such as cosmetics or facial cleansers that have skin whitening or wrinkle improvement effects.

Products to which the cosmetic composition of the present invention can be added include, for example, BB cream, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, and ultraviolet rays. There are cosmetics such as blocking cream, moisture cream, hand cream, foundation, essence, nutritional essence, and mask pack, as well as soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier ingredient. You can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. , butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, fatty esters of glycerol, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

Hereinafter, preferred embodiments are presented to aid understanding of the present invention. However, the following examples are merely illustrative of the present invention, and it is clear to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention. It is natural that such variations and modifications fall within the scope of the attached patent claims.

[Example]

Preparation Example A: Mok Danbark Extract

Cut the wood bark into small pieces, perform hot water extraction at 100°C for 4 hours at a ratio of 1:10 (w/v) with water, filter through non-woven fabric to remove impurities, and concentrate under reduced pressure to have a solid content of 15% or more to prepare wood bark extract. did.

Preparation Example B: Extract of Gyejo axis

An extract of the extract of the extract of the extract was prepared under the same conditions as Preparation Example A, except that the bark of the extract was used instead of the bark of the extract.

Preparation Example C: Yeongyo Extract

Forsythiae Fructus extract was prepared by extraction under the same conditions as Preparation Example A, except that Forsythiae Fructus was used instead of the bark of the tree.

Preparation Example 1: Lactobacillus Plantarum culture using Dogo hot spring water

Lactobacillus plantarum strain was inoculated with 1 ml of Lactobacillus plantarum (10 ⁸ CFU/ml) in a mixed medium mixed with 70 ml of MRS Broth liquid medium and 30 ml of Dogo Hot Spring water, and incubated at 37°C for 30 hours. After fermentation, the culture was disrupted with a homogenizer to obtain a Lactobacillus plantarum culture.

Preparation Example 2: Bifidobacterium longum culture using Dogo hot spring water

A Bifidobacterium longum culture was obtained by culturing under the same conditions as Preparation Example 1, except that Bifidobacterium longum strain was used instead of Lactobacillus plantarum.

Preparation Example 3: Lactobacillus plantarum culture using Dogo hot spring water + herbal medicine complex extract

Preparation Example 1, except that instead of the mixed medium mixed with 70 ml of MRS Broth liquid medium and 30 ml of Dogo Hot Spring water, a mixed medium mixed with 70 ml of MRS Broth liquid medium, 27 ml of Dogo Hot Spring water, and 3 ml of herbal medicine complex extract was used. Lactobacillus plantarum culture was obtained under the same conditions. Here, the herbal medicine complex extract is a mixture of the Mokdan bark extract from Preparation Example A, the Gyejool axil extract from Preparation Example B, and the perilla extract from Preparation Example C at a volume ratio of 1:1:1.

Preparation Example 4: Bifidobacterium longum culture using Dogo hot spring water + herbal medicine complex extract

A Bifidobacterium longum culture was obtained by culturing under the same conditions as Preparation Example 3, except that Bifidobacterium longum strain was used instead of Lactobacillus plantarum.

Comparative Preparation Example 1: Lactobacillus Plantarum cell removal culture using Dogo hot spring water

After fermentation, the culture was prepared in the same manner as in Preparation Example 1, except that instead of treating the culture with a homogenizer to crush the cells, the culture was centrifuged to obtain a filtrate from which the cells were removed.

Comparative Preparation Example 2: Bifidobacterium longum cell removal culture using Dogo hot spring water

After fermentation, the culture was prepared in the same manner as Preparation Example 2, except that instead of treating the culture with a homogenizer to crush the cells, the culture was centrifuged to obtain a filtrate from which the cells were removed.

Comparative Preparation Example 3: Lactobacillus Plantarum cell removal culture using Dogo hot spring water + herbal medicine complex extract

After fermentation, the culture was prepared in the same manner as in Preparation Example 3, except that instead of treating the culture with a homogenizer to crush the cells, the culture was centrifuged to obtain a filtrate from which the cells were removed.

Comparative Preparation Example 4: Bifidobacterium cell removal longum culture using Dogo hot spring water + herbal medicine complex extract

A Bifidobacterium longum culture was obtained by culturing under the same conditions as Preparation Example 4, except that Bifidobacterium longum strain was used instead of Lactobacillus plantarum .

Example 1: Mixed culture using Dogo hot spring water

A mixed culture was prepared by mixing the Lactobacillus plantarum culture of Preparation Example 1 and the Bifidobacterium longum culture of Preparation Example 2 at a volume ratio of 1:1.

Example 2: Mixed culture using Dogo hot spring water + complex herbal medicine

A mixed culture was prepared by mixing the Lactobacillus Plantarum culture of Preparation Example 3 and the Bifidobacterium longum culture of Preparation Example 4 at a volume ratio of 1:1.

Comparative Example 1: Mixed culture using Asan hot spring water

A mixed culture was prepared under the same conditions as in Example 1, except that Asan hot spring water was used instead of Dogo hot spring water.

Comparative Example 2: Mixed culture using Asan hot spring water + complex herbal medicine

A mixed culture was prepared under the same conditions as in Example 1, except that Asan hot spring water was used instead of Dogo hot spring water.

Comparative Example 3: Mixed culture using Onyang hot spring water

A mixed culture was prepared under the same conditions as in Example 1, except that Onyang Hot Spring water was used instead of Dogo Hot Spring water.

Comparative Example 4: Mixed culture using Onyang hot spring water + complex herbal medicine

A mixed culture was prepared under the same conditions as in Example 1, except that Onyang Hot Spring water was used instead of Dogo Hot Spring water.

Comparative Example 5: Bacteria removal mixed culture using Dogo hot spring water

A mixed culture was prepared by mixing the Lactobacillus Plantarum cell-removed culture of Comparative Preparation Example 1 and the Bifidobacterium longum cell-removed culture of Comparative Preparation Example 2 at a volume ratio of 1:1.

Comparative Example 6: Bacteria removal mixed culture using Dogo hot spring water and complex herbal medicine

A mixed culture was prepared by mixing the Lactobacillus Plantarum cell-removing culture of Comparative Preparation Example 3 and the Bifidobacterium longum cell-removing culture of Comparative Preparation Example 4 at a volume ratio of 1:1.

Comparative Example 7: Mixed culture using Dogo hot spring water + Mokdan bark

A mixed culture was prepared under the same conditions as in Example 2, except that the extract from Preparation Example A was used alone instead of the complex herbal medicine extract.

Comparative Example 8: Mixed culture using Dogo hot spring water + gradation axis

A mixed culture was prepared under the same conditions as in Example 2, except that the Gyejo axis extract of Preparation Example B was used alone instead of the complex herbal medicine extract.

Comparative Example 9: Mixed culture using Dogo Hot Spring water + Yeongyo

A mixed culture was prepared under the same conditions as in Example 2, except that the Yeonji extract of Preparation Example C was used alone instead of the complex herbal medicine extract.

Comparative Example 10: Mixed culture using Dogo hot spring water + Mokdanpi/Gyejo axis

A mixed culture was prepared under the same conditions as in Example 2, except that instead of the complex herbal medicine extract, an extract obtained by mixing the Mokdan bark extract from Preparation Example A and the Gyejo axis extract from Preparation Example B at a volume ratio of 1:1 was used.

Comparative Example 11: Mixed culture using Dogo hot spring water + Mokdanpi/Yeongyo

A mixed culture was prepared under the same conditions as in Example 2, except that instead of the complex herbal medicine extract, an extract obtained by mixing the Mokdan bark extract of Preparation Example A and the Yeongyo extract of Preparation Example C at a volume ratio of 1:1 was used.

Comparative Example 12: Mixed culture using Dogo hot spring water + gray axis/bridge

A mixed culture was prepared under the same conditions as in Example 2, except that instead of the complex herbal medicine extract, an extract obtained by mixing the Yeongyo extract of Preparation Example B and the Yeongyo extract of Preparation Example C at a volume ratio of 1:1 was used.

Comparative Example 13: Single culture using Dogo hot spring water + complex herbal medicine

Lactobacillus Plantarum single culture prepared according to Preparation Example 3 was used.

Comparative Example 14: Single culture using Dogo hot spring water + complex herbal medicine

Bifidobacterium longum single culture prepared according to Preparation Example 4 was used.

The culture preparation conditions of Preparation Examples 1 to 4, Comparative Preparation Examples 1 to 4, Examples 1 and 2, and Comparative Examples 1 to 12 are summarized in Tables 1 and 2 below.

division Hot spring water with badge Fermentation strain Herbal medicine including medium Whether the culture contains bacteria Manufacturing Example 1 Dogo hot spring water Lactobacillus Plantarum doesn't exist include Production example 2 Dogo hot spring water Bifidobacterium longum doesn't exist include Production example 3 Dogo hot spring water Lactobacillus Plantarum 3 types of combination
(Production Examples A, B, C) include Production example 4 Dogo hot spring water Bifidobacterium longum 3 types of combination
(Production Examples A, B, C) include Comparative Manufacturing Example 1 Dogo hot spring water Lactobacillus Plantarum doesn't exist Not included Comparative Manufacturing Example 2 Dogo hot spring water Bifidobacterium longum doesn't exist Not included Comparative Manufacturing Example 3 Dogo hot spring water Lactobacillus Plantarum 3 types of combination
(Production Examples A, B, C) Not included Comparative Manufacturing Example 4 Dogo hot spring water Bifidobacterium longum 3 types of combination
(Production Examples A, B, C) Not included

division Hot spring water with badge fermentation strain Herbal medicine including medium Whether the culture contains bacteria Example 1 Dogo hot spring water 2 types doesn't exist include Example 2 Dogo hot spring water 2 types 3 types of combination
(Production Examples A, B, C) include Comparative Example 1 Asan hot spring water 2 types doesn't exist include Comparative Example 2 Asan hot spring water 2 types 3 types of combination
(Production Examples A, B, C) include Comparative Example 3 Onyang hot spring water 2 types doesn't exist include Comparative Example 4 Onyang hot spring water 2 types 3 types of combination
(Production Examples A, B, C) include Comparative Example 5 Dogo hot spring water 2 types doesn't exist Not included Comparative Example 6 Dogo hot spring water 2 types 3 types of combination
(Production Examples A, B, C) Not included Comparative Example 7 Dogo hot spring water 2 types 1 type
(Manufacturing Example A: Mok Danskin) include Comparative Example 8 Dogo hot spring water 2 types 1 type
(Manufacturing example B: gradation axis) include Comparative Example 9 Dogo hot spring water 2 types 1 type
(Manufacturing example C: soft bridge) include Comparative Example 10 Dogo hot spring water 2 types 2 types
(Manufacturing example A, B: wooden hem, gray scale) include Comparative Example 11 Dogo hot spring water 2 types 2 types
(Manufacturing example A, C: wooden lining, soft bridge) include Comparative Example 12 Dogo hot spring water 2 types 2 types
(Manufacturing example B, C: Gradient shaft, bridge) include Comparative Example 13 Dogo hot spring water single
(Lactobacillus plantarum) 3 types of combination
(Production Examples A, B, C) include Comparative Example 14 Dogo hot spring water single
(Bifidobacterium longum) 3 types of combination
(Production Examples A, B, C) include

For reference, the results of comparing the mineral components and contents of Dogo Hot Spring water used as a medium component in the examples, which are the three major hot spring waters distributed in the Asan region, and Asan hot spring water and Onyang hot spring water used in the comparative examples are shown in Table 3 below. . The results in Table 3 are quoted from a publicly announced project report related to “Development of skin improvement cosmetics using hot spring water in Chungnam region.”

[Experimental example]

All statistical analyzes were performed using SPSS version 18.0 (IBM, Chicago, IL, USA). Differences in mean values between experimental groups (p <0.05) were determined using one-way ANOVA with Duncan's post-hoc test. Each experimental data was expressed as mean ± standard deviation (SD).

Experimental Example 1: Cytotoxicity analysis

Cytotoxicity was measured using the MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction method. The cultured HaCaT cells were mixed well in DMEM medium and adjusted to 1x10 ⁵ cells/mL, and then 1 mL of prepared cells were added to a 24-well culture plate and cultured for 3 days. When the cells had grown to about 80%, samples of Examples 1 and 2 and Comparative Examples 1 to 4 (0, 25, 50, 100, 300, 400, 500, 1000) were added at different concentrations to medium without fetal bovine serum and antibiotics. After treatment with ㎍/㎖), the cells were cultured for an additional 24 hours, and the survival rate of the cells upon completion of the culture was measured using the MTT reduction method.

Approximately 1/10 of the volume of growth medium was added with MTT solution (5 mg/ml) to each well, and then cultured at 37°C for another 4 hours. Afterwards, the medium was removed, being careful not to lose the formazan produced by reducing MTT, and left at room temperature for 30 minutes to completely remove the remaining medium. Then, the sample was dissolved using DMSO and then analyzed at 570 nm. The absorbance was measured. Cell survival rate was calculated according to Equation 1 below, and the results are shown in Table 4.

[Equation 1]

Cell viability (%) = [Absorbance of sample treatment group/Absorbance of control group] x 100

division Concentration (㎍/㎖) 10 100 Untreated 100±1.1 Example 1 97.8±1.3 93.1±1.4 Example 2 95.7±1.9 90.3±0.5 Comparative Example 1 98.2±0.5 92.1±0.9 Comparative Example 2 96.1±2.4 91.3±1.0 Comparative Example 3 97.7±0.5 92.8±1.1 Comparative Example 4 96.3±1.0 90.5±0.8

Accordingly, it was confirmed that not only the cultures according to Examples 1 and 2 of the present invention, but also the cultures of Comparative Examples 1 to 4 can be safely used on human skin. Since the cultures of Comparative Examples 5 to 12 were included in the components of Examples 1 and 2 or other comparative examples, separate cytotoxicity was not performed.

Experimental Example 2: Analysis of the effect of inhibiting MMP-1 synthesis

CCD-986sk was cultured in a 35 mm dish at a concentration of 1.5X10 ⁵ cells/ml until reaching approximately 80% confluency. After removing the original medium, it was washed with PBS to remove serum components in the medium, and then UVA (6.3 J/cm2) was irradiated for 24 hours using a UV light source (Coralife, 35W, UV) equipped with a filter. . After UVA irradiation, the samples of Examples or Comparative Examples of the present invention were administered at concentrations of 10 and 100 μg/ml, respectively, to DMEM medium without FBS, and then cultured for 24 hours. The medium cultured in this way was dispensed into a 96 well plate and reacted at 4°C overnight. Measurement of MMP-1 expression level induced by UVA irradiation was performed using the method used by Dunsmore et al.

First, the medium was washed three times with TBS (phosphate buffered saline + 0.05% Tween20) and then blocked with 3% PBS at 37°C for 1 hour. Afterwards, a solution of the primary antibody (monoclonal anti-MMP-1 antibody) diluted with blocking buffer at a volume ratio of 1:3000 was added and reacted at 37°C for 90 minutes. After washing with PBS, a solution of secondary antibody (alkaline phosphatase conjugated goat anti-mouse IgG) diluted with blocking buffer at a volume ratio of 1:3,000 was added and reacted at 37°C for 90 minutes. After washing with PBS, alkaline phosphatase substrate solution (1 mg/ml, p-mittrophenylphosphate in diethanolamine buffer) was added and reacted at room temperature for 30 minutes, and then the reaction was stopped with 3N NaOH. Next, absorbance was measured at 405 nm using a microplate reader, and the results are shown in Table 5 below.

division Concentration (㎍/㎖) 10 100 Untreated 8760±15 Example 1 6820±42 5320±94 Example 2 4802±82 3306±38 Comparative Example 1 7140±90 6206±72 Comparative Example 2 5390±101 3828±45 Comparative Example 3 6900±42 6724±88 Comparative Example 4 5208±70 4109±54 Comparative Example 5 7840±110 6722±72 Comparative Example 6 6257±75 6563±49 Comparative Example 7 6724±40 5327±49 Comparative Example 8 6520±66 5890±121 Comparative Example 9 6054±79 5807±20 Comparative Example 10 5520±61 5090±102 Comparative Example 11 6254±33 5944±51 Comparative Example 12 6800±43 5583±99 Comparative Example 13 7504±47 7210±43 Comparative Example 14 7663±58 7123±28

According to this, the culture of Example 2, which included a mixed culture using two types of fermentation strains and three types of complex herbal medicines in the culture, was measured to have a very high inhibitory effect on the synthesis of MMP-1 protein compared to the other experimental groups, and 3 Even when one or two types of herbal medicines rather than complex herbal medicines were included in the medium, the inhibitory effect on MMP-1 protein synthesis did not show a significant difference compared to Example 1 in which the herbal medicines were excluded. In addition, Comparative Examples 2 and 4, which contained three types of composite herbal medicine and Asan or Onyang hot spring water, also showed excellent effects in inhibiting the synthesis of MMP-1 protein, but showed a relatively lower effect compared to Example 2.

In addition, in the case of the cultures of Comparative Examples 5 and 6 in which the bacteria were removed from the culture, the inhibitory effect on the synthesis of MMP-1 protein was measured to be lower than that in Examples 1 and 2 containing the bacteria, and when only one type of fermentation strain was used, It appears to be effective in improving wrinkles as it has the lowest inhibitory effect on the synthesis of MMP-1 protein, a collagen decomposing enzyme.

Experimental Example 3: Analysis of collagen synthesis promotion effect

In order to confirm the skin wrinkle improvement effect of the cosmetic compositions of Examples and Comparative Examples, the production amount of collagen, a protein that maintains skin elasticity, was measured. Fibroblasts were distributed in a ⁴⁸ well plate at a density of 1 Cultured in serum medium for an additional 24 hours. After incubation, the supernatant from each well was collected, and the amount of procollagen type I C-peptide (PICP) was measured using a procollagen type I C-peptide EIA kit (MK101, Takara, Japan) and converted to ng/ml. , the amount of collagen synthesized was measured, and the results are shown in Table 6.

division Concentration (㎍/㎖) 50 100 Untreated 40.1±0.8 Example 1 60.8±1.7 75.9±1.4 Example 2 82.2±1.2 92.4±0.8 Comparative Example 1 49.8±1.2 52.9±1.5 Comparative Example 2 77.7±0.8 88.2±1.3 Comparative Example 3 47.5±1.1 55.7±0.3 Comparative Example 4 80.6±0.5 90.2±0.7 Comparative Example 5 49.5±1.3 54.7±0.4 Comparative Example 6 50.6±0.8 52.8±0.8 Comparative Example 7 61.8±1.0 73.5±1.6 Comparative Example 8 58.7±0.6 65.8±2.1 Comparative Example 9 58.9±1.8 67.0±0.7 Comparative Example 10 68.3±1.4 79.0±0.6 Comparative Example 11 62.2±1.1 67.6±1.2 Comparative Example 12 60.8±0.7 64.0±1.4 Comparative Example 13 43.9±1.1 47.8±0.7 Comparative Example 14 42.3±1.2 48.0±0.5

Accordingly, it was confirmed that the culture prepared according to Example 2 of the present invention had a very excellent collagen production promotion effect compared to the untreated group and comparative examples. On the other hand, it was confirmed that the culture using one type of strain or the culture without using three types of composite herbal medicine showed a relatively low collagen production ability compared to Example 2. In addition, in the case of Comparative Example 2, which contained three types of complex herbal medicines and contained Asan Hot Spring water rather than Dogo Hot Spring water, and Comparative Example 4, which contained Onyang Hot Spring water, the collagen production ability was shown to be higher than that of the other comparative examples, but in Example 2 Compared to this, it showed relatively low collagen production ability.

Experimental Example 4: Tyrosinase inhibition activity analysis

The tyrosinase inhibitory ability of the cultures of Examples and Comparative Examples was measured by the method of Yagi et al. (1986). Melanin exists in the basal layer of the epidermis, and melanin synthesis is regulated by tyrosinase in the intracellular melanosomes of melanocytes. Therefore, inhibiting tyrosinase activity can reduce melanin synthesis and pigmentation. 0.2 ml of 110 Unit/ml mushroom tyrosinase was added to a mixture of 0.2 ml of substrate solution and 0.1 ml of sample solution in which 10 mM L-DOPA was dissolved in 0.5 ml of 1.15M sodium phosphate buffer at pH 6.8 in a test tube. Afterwards, the reaction was carried out at 25°C for 2 minutes, and the absorbance of DOPA chrome produced in the reaction solution was measured at 490 nm using a microplate reader. The tyrosinase inhibitory activity was expressed as the rate of decrease in absorbance of the group with and without the addition of the sample solution, and the results are shown in Table 7 below.

division Treatment concentration (%(w/v)) 50 100 Example 1 3.3±0.9 6.4±1.3 Example 2 7.9±0.7 13.7±1.0 Comparative Example 1 3.1±0.8 8.5±1.7 Comparative Example 2 6.5±0.2 11.5±0.4 Comparative Example 3 3.8±0.4 8.7±0.7 Comparative Example 4 6.1±0.6 10.9±0.6 Comparative Example 5 2.1±0.7 6.8±0.7 Comparative Example 6 3.2±0.4 8.1±0.3 Comparative Example 7 3.3±0.2 6.7±0.1 Comparative Example 8 2.4±0.3 6.8±1.1 Comparative Example 9 2.9±0.9 5.9±0.6 Comparative Example 10 4.8±0.2 8.6±0.1 Comparative Example 11 4.0±0.4 8.6±0.3 Comparative Example 12 4.2±0.7 8.4±0.6 Comparative Example 13 3.8±0.2 6.7±0.8 Comparative Example 14 3.6±0.4 7.1±0.5

According to this, the culture of Example 2 of the present invention was found to have the highest tyrosinase inhibitory activity, and Comparative Example 2, which included three types of composite herbal medicine and Asan Hot Spring water rather than Dogo Hot Spring water, included Onyang Hot Spring water. In the case of Comparative Example 4, the tyrosinase inhibitory activity was measured at a higher level compared to other comparative examples, but was measured at a relatively low level compared to Example 2. On the other hand, when only one fermentation strain or two or less types of herbal medicine were used, the tyrosinase inhibitory activity was measured to be relatively low.

Experimental Example 5: Analysis of melanin production inhibition ability

B16F1 cells, which are mouse-derived melanocytes, produce melanin. Melanin is biosynthesized in melanosome, an organelle within melanocytes present in the basal layer of the epidermis, and the amount produced can be compared by measuring the absorbance in the visible light range. After inoculating B16F1 cells, the sample was processed for 3 days, the medium was removed, the cells were washed with PBS, 1 ml of 1 N NaOH was added to each well, and the cell membrane was dissolved by stirring, thereby dissolving the melanin component and releasing it in the visible light region. The amount of melanin produced was measured by measuring the absorbance at 400 nm, which is the blue visible light range with the shortest wavelength.

The amount of melanin was measured by expressing the absorbance per unit cell (10 ⁶ cells), and the amount of melanin production relative to the control group (untreated group) was calculated as an inhibition rate (%), and the results are summarized in Table 8.

Classification (unit: %) Concentration (㎍/㎖) 50 100 Untreated 40.5±0.6 Example 1 46.7±0.4 53.7±0.4 Example 2 68.2±0.8 75.5±0.9 Comparative Example 1 44.5±0.4 47.7±0.2 Comparative Example 2 60.0±1.2 71.2±0.8 Comparative Example 3 43.7±0.9 48.5±1.0 Comparative Example 4 61.4±0.5 72.1±0.2 Comparative Example 5 36.2±0.9 45.7±0.6 Comparative Example 6 42.0±0.2 48.7±0.4 Comparative Example 7 45.0±0.7 53.6±0.9 Comparative Example 8 42.1±1.2 49.3±0.4 Comparative Example 9 48.0±1.1 48.7±1.2 Comparative Example 10 50.1±0.8 58.6±0.9 Comparative Example 11 42.1±0.5 49.3±0.2 Comparative Example 12 42.8±0.5 48.6±0.6 Comparative Example 13 38.9±0.9 47.7±0.5 Comparative Example 14 36.8±0.4 49.1±0.5

According to this, the culture of Example 2 was found to have the best ability to inhibit melanin production, Comparative Example 2 containing three types of composite herbal medicine and containing Asan Hot Spring water rather than Dogo Hot Spring water, and Comparative Example 4 containing Onyang Hot Spring water. In this case, the melanin production inhibition ability was measured at a higher level compared to other comparative examples, but was relatively low compared to Example 2. In addition, when only one type of fermented strain was used or two or less types of herbal medicine were used, the ability to inhibit melanin production was found to be significantly reduced.

A formulation example of a cosmetic composition suitable for applying the present invention is presented.

Formulation Example 1: Toner

A formulation example of a lotion using the cosmetic composition of Example 2 is shown in Table 9 below.

ingredient Content (% by weight) Composition of Example 2 5.0 glycerin 6.0 1,3-butylene glycol 3.0 PEG 1500 1.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium Hyaluronate 5.0 ethanol 10.0 Polysorbate 20 0.2 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount Sum 100

Formulation Example 2: Lotion

A formulation example of a lotion using the cosmetic composition of Example 2 is shown in Table 10 below.

ingredient Content (% by weight) Composition of Example 2 3.0 propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocopheryl Acetate 3.0 liquid paraffin 5.0 squalane 3.0 macadami nut oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxy vinyl polymer 1.0 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount Sum 100

Formulation Example 3: BB Cream

A formulation example of a BB cream using the cosmetic composition of Example 2 is shown in Table 11 below.

ingredient Content (% by weight) Composition of Example 2 1.0 Lipophilic glycerin monostearate 1.5 cetearyl alcohol 1.5 stearic acid 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 Isostearyl isostearate 5.0 squalane 5.0 mineral oil 35.0 Dimethicone 0.5 Hydroxyethylcellulose 0.12 glycerin 6.0 Triethanolamine 0.7 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount Sum 100

Formulation Example 4: Essence

A formulation example of an essence using the cosmetic composition of Example 2 is shown in Table 12 below.

ingredient Content (% by weight) Composition of Example 2 1.5 glycerin 10.0 Betaine 5.0 PEG 1500 2.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethylcellulose 0.1 Sodium Hyaluronate 8.0 Carboxy vinyl polymer 0.2 Triethanolamine 0.18 octyldodecanol 0.3 Octyldodeceth-16 0.4 ethanol 6.0 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount Sum 100

Formulation Example 5: Emulsion for mask pack

A formulation example of a mask pack emulsion using the cosmetic composition of Example 2 is shown in Table 13 below.

ingredient Content (% by weight) Composition of Example 2 1.0 polyvinyl alcohol 15.0 cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 Cyclodextrin 0.15 DL-panthenol 0.4 allantoin 0.1 Monoammonium glycyrrhizinate 0.3 nicotinamide 0.5 ethanol 6.0 PEG 40 Hydrogenated Castor Oil 0.3 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount Sum 100

Although the embodiments of the present invention have been described above, those skilled in the art can add, change, delete or add components without departing from the spirit of the present invention as set forth in the patent claims. The present invention may be modified and changed in various ways, and this will also be included within the scope of rights of the present invention.

Claims (9)

A culture containing a strain of the genus Lactobacillus cultured in a medium containing Dogo Hot Spring water; and
Contains as an active ingredient a culture containing a strain of the genus Bifidobacterium cultured in a medium containing Dogo Hot Spring water,
The medium containing the Dogo hot spring water includes Mokdan bark extract, Gyejo axis extract, and Yeongyo extract,
The strain of the Lactobacillus genus is Lactobacillus plantarum,
A cosmetic composition for skin whitening or wrinkle improvement, wherein the Bifidobacterium genus strain is Bifidobacterium longum. delete According to paragraph 1,
A cosmetic composition, characterized in that the herbal medicine extract is extracted with water, alcohol with 1 to 4 carbon atoms, or a mixed solvent thereof. According to paragraph 1,
A cosmetic composition characterized in that the medium containing the Dogo hot spring water contains 10 to 40% (v/v) of the Dogo hot spring water. According to paragraph 1,
A cosmetic composition characterized in that the medium containing the Dogo hot spring water contains 1 to 5% (v/v) of the herbal medicine extract, and the herbal medicine extract has a solid content of 10 to 20% (w/v). According to paragraph 1,
The Dogo hot spring water contains 55 to 65 mg/L of calcium (Ca), 25 to 35 mg/L of sodium (Na), 10 to 20 mg/L of silicon (Si), 0.001 to 0.05 mg/L of lithium (Li), and strontium ( A cosmetic composition comprising Sr) 1.0 to 2.0 mg/L. delete delete delete