KR102087904B1 - Cosmetic compositions containing bifida ferment lysate - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a bifidus extract. The composition comprises a bifidus extract, a star anise extract, a Hamamelis virginiana leaf extract, and a Phyllanthus emblica fruit extract, thereby having effects of alleviating skin wrinkles, improving skin whitening, and increasing a duration time.

Description

Cosmetic composition containing a bifidus extract {Cosmetic compositions containing bifida ferment lysate}

The present invention relates to a cosmetic composition including a bifidus extract having a skin wrinkle improvement effect, a whitening effect and a duration increase effect.

Skin aging is an irreversible phenomenon, a process of morphological, biochemical and physiological changes that parallels the aging of a human individual. Unlike the aging of other organs and organs in the body, skin aging is caused by extrinsin aging caused by UV, smoking, or other factors associated with lifestyle, in addition to intrinsic aging, a process of aging that is genetically determined. aging) process.

Skin aging due to exogenous and endogenous aging is characterized by clinical manifestations such as solar elastosis, dyschromatosis, roughness, and wrinkle formation. The anti-aging treatments in the field of skin care are focused on the improvement of the individual symptoms of skin aging. In addition to medical procedures and surgical treatments, the anti-aging mechanisms of functional products in the cosmetic field are also the same. can see.

The anti-wrinkle effect of cosmetics is based on the effect of improving the dermal layer by various active ingredients in addition to the temporary effect of moisturizing by moisturizing factors, and growth factor components such as retinol, vitamin C, and EGF, which are the raw materials of representative anti-wrinkle cosmetics. They are well known for their ability to improve wrinkles by increasing collagen angiogenesis from human dermal fibroblasts.

In order to prevent this phenomenon and maintain a healthier and more beautiful skin, by using physiologically active substances obtained from various known animals, plants, microorganisms, etc. in cosmetics, the unique function of the skin is maintained and the skin cells are activated by activating the skin. Efforts have been made to effectively inhibit aging.

In particular, many anti-aging and wrinkle improvement products using plant components have been developed. However, these plant extract-based cosmetics have a disadvantage that when applied to the skin, such as a paste applied to the solution as the solution evaporates, stiffness around the eyes occurs, and the average product effect duration is 6-7 hours per day. If you do outside activities all day long, the lasting power is almost gone.

In the field of cosmetics, research and development of functional cosmetics that have effects such as whitening, wrinkle improvement, and duration increase are being actively conducted. In particular, researches using natural materials are continued to avoid toxicity or irritation to the skin. .

Republic of Korea Patent No. 1743488 Republic of Korea Registered Patent No.

It is an object of the present invention to provide a cosmetic composition having a skin wrinkle improvement effect, a whitening effect and a duration increase effect, including a bifidus extract.

The cosmetic composition of the present invention for achieving the above object may include a bifidus extract, a star anise extract, a Virginia leaf extract and an Indian gooseberry fruit extract.

The cosmetic composition may further include a mixed extract of mulberry bark and sky root.

The bifidus extract may be a bifida ferment lysate (Bifida Ferment Lysate) which is an extract obtained by culturing the bifidus bacteria and crushing the cells with a homogenizer.

The star anise extract may be an extract obtained by fermenting star anise with bifidus bacteria.

The mixed extract of the mulberry bark and skytari root may be an extract fermented with a bifidus bacteria mixture of the mulberry bark and skytari root.

The mulberry bark and sky roots may be mixed in a weight ratio of 1: 0.2-0.8.

The cosmetic composition may be included as 5 to 30 parts by weight of star anise extract, 1 to 10 parts by weight of Virginia foliaceae extract and 10 to 40 parts by weight of Indian gooseberry fruit extract, based on 100 parts by weight of the bifidus extract, the mulberry bark and sky root The mixed extract may be mixed in an amount of 1 to 20 parts by weight based on 100 parts by weight of the bifidus extract.

The cosmetic composition is BB cream, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nourishing lotion, massage cream, nourishing cream, sunscreen cream, moisturizing cream, hand cream, foundation, essence, nutrition It can be used in combination with cosmetics selected from the crowd consisting of essences, mask packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

The cosmetic composition may be a composition having a skin wrinkle improvement effect, a whitening effect and a moisture retention duration increase effect.

The cosmetic composition of the present invention is composed of a natural material and is safe for the skin, and has an excellent skin wrinkle prevention and improvement effect, a whitening effect, and an improvement effect of sustaining moisture. More specifically, the composition of the present invention effectively inhibits the expression of MMP-1 and MMP-3 proteins directly acting on the production of skin wrinkles, and increases the expression of type I procollagen protein to prevent and improve skin wrinkles. Works effectively; Inhibits tyrosinase and inhibits the production of melamine, which can effectively act on whitening.

In addition, the cosmetic composition of the present invention hardly aggregates the cosmetic composition due to sweat or sebum when applied for a long time, and has excellent adhesion to the skin.

The present invention relates to a cosmetic composition including a bifidus extract having a skin wrinkle improvement effect, a whitening effect and a water retention duration increase effect.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

The cosmetic composition of the present invention includes a bifidus extract, a star anise extract, a virginia perennial leaf extract, and an Indian gooseberry fruit extract, and may further include a mixed extract of mulberry bark and skyroot.

The Bifidobacteria genus, specifically Bifidobacteria lysate, is a bacterium that is safe enough to produce useful metabolites for humans and to be used as a food raw material. The bifido extract of the present invention cultures the bifidus bacteria and disrupts cells with a homogenizer. Bifida ferment lysate containing the metabolic components, cytoplasm, cell wall components, polysaccharides, and the like of Bifidobacteria.

The bifidus extract improves wrinkles and skin tone and supplies moisture and nutrition.

In addition, the star anise extract is used as a bifidus extract and virginia foliaceae extract to help skin whitening and improve wrinkles, is a fermentation extract fermentation of star anise fruit with bifidus bacteria. If the star anis extract is used as it is without fermentation into bifidus bacteria, it may have an effect on skin whitening but skin problems and psoriasis may occur.

The star anise extract is used in 5 to 30 parts by weight, preferably 10 to 20 parts by weight based on 100 parts by weight of bifidus extract. When the content of the star anise extract is less than the lower limit, even when used in combination with the bifidus extract and the Virginia foliar leaf extract has no whitening and anti-wrinkle effect, if the upper limit exceeds the adhesion may be reduced.

In addition, the virginia foliaceae extract is used with a mixed extract of star anise extract and mulberry bark and sky roots to improve wrinkles and when used as a BB cream, etc., it is a substance that improves the persistence and adhesion even if a long time flows. Virginia fernium leaf extract is preferably used without fermentation when fermented by Bifidus bacteria because the durability and adhesion is lowered.

The Virginia Pyunfu leaf extract is used in 1 to 10 parts by weight, preferably 3 to 5 parts by weight based on 100 parts by weight of bifidus extract. If the content of the virginia perennial leaf extract is less than the lower limit, wrinkles do not improve even when used with a mixed extract of star anise extract and mulberry bark and skyweed root, and the persistence and adhesion may be lowered. This can happen.

In addition, the Indian gooseberry fruit extract is used as a bifidus extract to supply moisture to the skin and improve the whitening effect. The Indian gooseberry fruit extract is preferably used without fermentation when fermented with Bifidobacteria because the whitening effect may be lowered.

The Indian gooseberry fruit extract is used in an amount of 10 to 40 parts by weight, preferably 15 to 25 parts by weight, based on 100 parts by weight of bifidus extract. When the content of the Indian gooseberry fruit extract is less than the lower limit, even when used together with the bifidus extract, the amount of water supplied to the skin may be reduced and the whitening effect may be lowered, and when the upper limit is exceeded, skin trouble may occur.

In addition, the mixed extract of the mulberry bark and sky root root can be used with bifidus extract and Virginia foliaceae extract to maintain moisture for a long time on the skin, and when used as a BB cream, etc. As a substance to improve, it is a fermented extract obtained by fermenting a mixture of mulberry bark and sky root with bifidus. Erythema may occur when the mixture of the mulberry bark and sky roots is used as it is without fermentation into bifidus bacteria. In addition, if the mulberry bark and the sky roots are fermented without mixing, and then mixed afterwards, the water retention duration and adhesion may be significantly lower than when the two substances are mixed and fermented.

The mixture of mulberry bark and sky roots is a mixture of mulberry bark and sky roots in a weight ratio of 1: 0.2-0.8, preferably 1: 0.3-0.5. If the content of the sky mulberry root is less than the lower limit on the basis of the mulberry bark, the persistence and adhesion may be lowered, and when the upper limit is exceeded, skin dryness may be caused.

The mixed extract of the mulberry bark and sky root root is used in 1 to 20 parts by weight, preferably 5 to 10 parts by weight with respect to 100 parts by weight of bifidus extract. When the content of the mixed extract is less than the lower limit, even when used in conjunction with the bifidus extract and Virginia foliar leaf extract is difficult to exert a long-term effect, the sustainability and adhesion may be lowered, if the upper limit is exceeded, the adhesion is lowered and skin itching occurs Can be.

In the present invention, the term "skin wrinkle" refers to a line produced by skin attenuation, which may be caused by a gene, a decrease in collagen present in the dermis of the skin, or an external environment. In the present invention, "skin improvement" refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating wrinkles already generated.

The main functions of skin fibroblasts include the regeneration of damaged skin tissue through extracellular matrix synthesis and proliferation.Exogenous aging factors such as photoaging, accumulation of damage by free oxygen, and endogenous aging factors due to cell aging due to telomeres breakdown They lower the physiological activity of dermal fibroblasts in the dermis. Type I collagen, which accounts for more than 95% of the extracellular matrix of the skin, plays a very important part in the physiological activity of fibroblasts through interactions and signal exchanges with cell adhesion molecules and cytoskeleton. When the synthesis of is decreased, the amount of collagen in the skin tissue is reduced, thereby reducing the surface tension, thereby reducing skin elasticity and skin wrinkles, as well as reducing the biologically active functions including the proliferation and regeneration of cells. Cause a vicious cycle.

Therefore, collagen of dermal fibroblasts may be directly related to the repair or regeneration of tissues during skin regeneration, skin elasticity, skin wrinkle formation and skin damage. That is, if the synthesis of collagen or procollagen of skin fibroblasts or the synthesis of matrix metallo-proteinase (hereinafter referred to as 'MMP') that degrades collagen is inhibited, skin elasticity improvement, skin regeneration, Skin wrinkle improvement, wound healing, repair and regeneration of damaged skin tissue; And skin aging prevention.

The composition comprising the bifidus extract of the present invention is characterized by increasing the expression of type I procollagen protein and inhibiting the expression of MMP-1 protein. Specifically, the composition comprising the bifidus extract of the present invention increases the production of type I procollagen, while suppressing the expression of matrix metallo-proteinase, which promotes collagen degradation, the skin elasticity Increase, skin regeneration, skin wrinkle improvement, wound healing, repair and regeneration of damaged skin tissue; And skin anti-aging effects. That is, it may have an effect of preventing or improving skin wrinkles.

In addition, in the present invention, the term "whitening" means that the skin is beautiful and white, and the skin color of a person is determined by the concentration and distribution of melanin inside the skin. In addition to the genetic factors, solar ultraviolet rays, fatigue, stress, etc. It is also affected by environmental or physiological conditions. Melanin is synthesized in the melanocytes in the epidermal layer of the skin, and the enzyme tyrosinase acts on tyrosine, a type of amino acid, in the melanocytes (melanosome), the dopa (DOPA) and dopaquinone. It is made through non-enzymatic oxidation. When such synthesis of melanin occurs excessively in the skin, the skin tone is darkened, and the appearance of spots, freckles, and the like. Therefore, by inhibiting the synthesis of melanin pigment by inhibiting tyrosinase activity in the skin, not only can brighten the skin tone to realize skin whitening, but also hyperpigmentation of skin such as blemishes and freckles caused by ultraviolet rays, hormones and genetic causes Can improve deposition.

In addition, the cosmetic composition of the present invention may further include a water-soluble vitamin, a fat-soluble vitamin, a polymer peptide, a polymer polysaccharide, a sphingolipid and a seaweed extract.

The water-soluble vitamins may be any one that can be formulated in cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H And salts thereof (thiamine hydrochloride, sodium ascorbate salt) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the present invention. Included in water-soluble vitamins. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, microbial culture purification, enzymatic or chemical synthesis.

The fat-soluble vitamins may be any one that can be formulated in cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E, and the like, and derivatives thereof (ascorbic palmitate, ascorbic acid stearate). Bin, dipalmitinate, ascorbin acetate, dl-alpha tocopherol, nicotinic acid dl-alpha tocopherolvitamin E, DL-pantotenyl alcohol, D-pantothenyl alcohol, pantothenylethyl ether, etc.) are also included in the fat-soluble vitamins used in the present invention. do.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics, and preferably collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like. The polymer peptide can be purified and obtained by a conventional method such as a purification method of microbial culture solution, an enzyme method or a chemical synthesis method, or can be purified and used from natural products such as dermis such as pigs and cows and silk fibers of silkworms.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt can be normally purified from a mammal or fish.

The sphingolipide may be any compound as long as it can be incorporated into cosmetics, and preferably, ceramide, phytosphingosine, sphingose sugar lipid and the like can be given. The sphingolipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any one that can be formulated in cosmetics, but preferably may include brown algae extract, red algae extract, green algae extract, and the like, and calginin, arginic acid, arginic acid purified from these seaweed extracts. Sodium, potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

The cosmetic composition of the present invention may be blended with the above essential components, if necessary, with other ingredients normally formulated into cosmetics.

Examples of the blending component that may be added include oil or fat components, moisturizers, emollients, ultraviolet absorbers, pH adjusters, flavoring agents, blood circulation accelerators, cooling agents, limiting agents, purified water and the like.

Examples of the fat or oil components include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

Examples of the ester fats and oils include glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristin, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, and isocetyl isostearate, Butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristinate, isostearyl palmitate, octytilate octylate, isocetyl isostearate, diethyl sebacinate, Diisopropyl adipic acid, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylolpropane, trimethyl tripropol triisostearate, pentaelyl tetraethyl hexanoate Slitol, Cetyl Caprylate, Decyl Laurin, Hexyl Lauric Acid, Mycetic Decyl, Myristin Myristyl, Myristin Cetyl, Stearyl Stearate, Decyl Oleate, Ricinooleic Acid Cetyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoic acid cetostearyl, 2-ethylhexanoic acid stearyl, hexyl isostearic acid, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol dicaprylic acid, neopentyl glycol dicapric acid Neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitine, glyceryl triisostearate, octyl dodecyl neopentane, isostearyl octanoate, octyl isonanoate , Hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, octyldecyl isostearate, polygly Linoleic acid ester, polyglycerol isostearic acid ester, triisoceyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, octyl lactate, octyl lactate, triethyl citrate, acetyl triethyl citrate, Acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, diethyl 2-ethylhexyl, diisobutyl adipicate, diisopropyl sebacinate, dioctyl sebacate, stearic acid Cholesteryl, isostearic acid cholesteryl, hydroxystearate cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitsteryl, oleic acid pitsteryl, 12-steloylhydroxy Isocetyl Stearate, 12-Steloylhydroxystearate Stearyl, 12-Steloylhydroxystearate Isostea And the like ester, such as.

Examples of the hydrocarbon-based oils and fats include hydrocarbon oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and vaseline.

Examples of the silicone-based fats and oils include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane methylcetyloxysiloxane copolymer, dimethylsiloxane methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as said fluorine-based fat or oil.

The animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, cottonseed oil, palm oil, Cucumber nut oil, wheat germ oil, rice germ oil, shea butter, moon colostrum oil, marker demia nut oil, egg yolk oil, tallow, horse oil, mink oil, orange rape oil, jojoba oil, canderry wax, carnauba wax, liquid ranol And animal or plant fats and oils such as hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

As the water-soluble low molecular moisturizer, serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), Polypropylene glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be mentioned.

Examples of the fat-soluble polymers include polyvinylpyrrolidone eicosene copolymer, polyvinylpyrrolidone hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, polymer silicone, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl ester, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate, Cinnamic acid benzyl, paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexane glyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture , Urocanoic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone disulfonate, dihydroxy Benzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2'-ethylhexyl-1'-oxy) -1,3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium fmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium fmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Cosmetics prepared by containing the composition of the present invention may take the form of solutions, emulsions, viscous mixtures and the like.

In addition, the components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the components as an active ingredient, for example, conventional auxiliaries such as stabilizers, pigments and natural flavors and It may further comprise a carrier.

The composition of the present invention may be used in various ways, such as cosmetics or face wash having a skin wrinkle improvement effect, a whitening effect and a duration increase effect.

As a product to which the composition of the present invention can be added, for example, BB cream, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, sunscreen Cosmetics such as creams, moisturizer creams, hand creams, foundations, essences, nutrition essences, mask packs, and soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal carriers, plant fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

Hereinafter, preferred examples are provided to help the understanding of the present invention, but the following examples are merely for exemplifying the present invention, and various changes and modifications within the scope and spirit of the present invention are apparent to those skilled in the art. It is natural that such variations and modifications fall within the scope of the appended claims.

Example 1.

A cosmetic composition was prepared including 100 parts by weight of bifidus extract, 12 parts by weight of star anise extract, 5 parts by weight of Virginia foliate extract and 17 parts by weight of Indian gooseberry fruit extract.

The bifidus extract was purchased from Aju Cosmetics Co., Ltd., and the Staranis extract was inoculated with 1 ml of Bifidus strain (Difco BL agat, Ref 210095 activated 10 ⁸ CFU / ml) in 10% solution. After fermentation at 37 ° C. for 2 days, 1 kg of purified water was added to the fermented product, and then obtained by ultrasonic extraction (Sonification) for 30 minutes using an ultrasonic extractor. In addition, the Virginia pianaceae leaf extract and the Indian gooseberry fruit extract was obtained by adding 1 kg of purified water to each of the Virginia prunus leaf and Indian gooseberry fruit, and then ultrasonically extracted for 30 minutes using an ultrasonic extractor.

Example 2. Adding a Mixed Extract of Mulberry Bark and Skyweed Root

Example 1 was carried out in the same manner as in Example 1, to the composition of Example 1 was added to the mixed extract of mulberry bark and sky root to 8 parts by weight to prepare a cosmetic composition.

The mixed extract of the mulberry bark and sky root roots is bifidus strain (Difco BL agat, Ref 210095 10% solution activated bifidus bacteria in 100 ml of a mixture of mulberry bark and sky root roots in a weight ratio of 1: 0.5 10 ⁸ CFU / ml 1 ml was inoculated, fermented at 37 ° C. for 2 days, and then 1 kg of purified water was added to the fermented product, followed by ultrasonic extraction for 30 minutes using an ultrasonic extractor.

Comparative Example 1. Ozone extract

The same procedure as in Example 1 was carried out, but the cosmetic composition was prepared without using a star anise extract.

Comparative Example 2. Star anise extract _ skipped fermentation

Example 1 was carried out in the same manner as in Example 1, but instead of the fermented staris extract fermented by a bifidus, a cosmetic composition was prepared using the fermented staris extract.

The fermented star anise extract was obtained by adding 1 kg of purified water to star anis and then ultrasonically extracting for 30 minutes using an ultrasonic extractor.

Comparative Example 3 Virginia Prunus Leaf Extract Omitted

The same procedure as in Example 1 was carried out, but the cosmetic composition was prepared without using the Virginia leaf extract.

Comparative Example 4. Indian Gooseberry fruit extract omitted

The same procedure as in Example 1 was carried out, but a cosmetic composition was prepared without using an Indian gooseberry fruit extract.

<Test Example>

All statistical analyzes were performed using SPSS version 18.0 (IBM, Chicago, IL, USA). One-way ANOVA using Duncan's post-hoc test was used to determine the difference in mean values between the experimental groups (p <0.05). Each experimental data is expressed as mean ± standard deviation (SD).

Test Example 1. MTT assay

Cytotoxicity was measured using MTT 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide) reduction method.

The cultured HaCaT cells were mixed well in DMEM medium, adjusted to 1 × 10 ⁵ cells / mL, and then 1 mL of the prepared cells were added to 24-well culture plates and cultured for 3-4 days. When the cells grew about 80%, treated with fetal calf serum and antibiotic-free medium with various concentrations of samples (0, 25, 50, 100, 300, 400, 500, 1000 μg / mL), and then 24 hours. The culture was further performed, and the survival rate of the cultured cells was measured using the MTT reduction method.

MTT solution (5 mg / mL) was added to each well in about 10/10 of the growth medium volume, and then incubated for 4 hours at 37 ° C. Then, be careful not to go out formazan produced by reducing MTT along the medium, remove the medium, and leave the medium for 30 minutes to completely remove the remaining medium, and then dissolved the sample using DMSO and absorbance at 570 nm Was measured. Cell viability was calculated as in Equation 1 below, and is shown in Table 1 below.

[Equation 1]

% Cell viability = [absorbance of sample treated / absorbed of control] x 100

division Concentration (µg / ml) 10 100 No treatment 100 ± 1.1 Example 1 97.8 ± 2.5 92.1 ± 1.3 Example 2 96.1 ± 0.6 91.3 ± 2.9 Comparative Example 1 96.9 ± 1.8 90.4 ± 0.8 Comparative Example 2 97.1 ± 0.7 91.7 ± 1.2 Comparative Example 3 96.3 ± 1.1 89.8 ± 1.0 Comparative Example 4 95.6 ± 2.4 90.5 ± 0.9

As shown in Table 1, it was confirmed that the compositions of Comparative Examples 1 to 4 as well as the cosmetic composition prepared according to Examples 1 and 2 of the present invention can be used safely on human skin.

Test Example 2 Suppression of Wrinkle Formation

2-1. Inhibition of Synthesis of MMP-1 Protein

CCD-986sk was incubated at a concentration of 1.5 × 10 ⁵ cells / ml until reaching about 80% confluency in a 35 mm dish. And after removing the original medium and washed with PBS to remove the serum component, and then irradiated with UVA (6.3J / ㎠) for 24 hours using a UV light source (Coralife, 35W, UV) equipped with a filter (凋謝).

After UVA irradiation, the samples according to Examples or Comparative Examples of the present invention were administered in DMEM medium without FBS, at concentrations of 0.1, 10, and 100 ug / ml, respectively, and then cultured for 24 hours. The cultured medium was dispensed into 96 well plates and reacted at 4 ° C. overnight.

MMP-1 expression level induced by UVA irradiation was carried out using the method used by Dunsmore et al.

First, the medium was washed three times with TBS (phosphate buffered saline + 0.05% Tween20), and then blocked with 3% PBS at 37 ° C. for 1 hour. Thereafter, a solution diluted in a weight ratio of 1: 3000 with a blocking buffer of a primary antibody (monoclonal anti-MMP-1 antibody) was added and reacted at 37 ° C. for 90 minutes. After washing with PBS, a solution diluted with a weight ratio of 1: 3,000 as a blocking buffer using a secondary antibody (alkaline phosphatase conjugated Goat anti-mouse IgG) was added and reacted at 37 ° C. for 90 minutes. After washing with PBS, alkaline phosphatase substrate solution (1 mg / ml, p-mitrophenylphosphate in diethanolamine buffer) was added thereto, followed by reaction at room temperature for 30 minutes, and then the reaction was stopped with 3N NaOH. Next, the absorbance was measured at 405 nm using a microplate reader.

division Concentration (µg / ml) 10 100 No treatment 8620 Example 1 6410 5020 Example 2 5920 4610 Comparative Example 1 7420 7100 Comparative Example 2 6950 6630 Comparative Example 3 7150 7050 Comparative Example 4 7060 6850

As shown in Table 2, the cosmetic compositions prepared according to Examples 1 and 2 of the present invention, compared to the untreated group and the compositions of Comparative Examples 1 to 4 effectively inhibit the production of MMP-1 protein to prevent skin wrinkles And it can be effectively used for improvement.

In particular, it was confirmed that the cosmetic composition of Example 2 more effectively inhibits the production of MMP-1 protein than the cosmetic composition of Example 1.

2-2. Promote the synthesis of type 1 procollagen protein

In order to confirm the skin wrinkle improvement effect of the cosmetic composition of the Examples and Comparative Examples, the amount of protein collagen to maintain the elasticity of the skin was measured.

Fibroblasts were dispensed in 48 well plates at a density of 1 × 10 ⁴ cells / well, incubated for 24 hours in DMEM medium containing 10% FBS, and then the samples of Examples or Comparative Examples were added at 50 and 100 μg / mL. Incubated for 24 hours in serum medium. After incubation, the supernatant of each well was collected, and the procollagen type I C-peptide (PICP) was measured using a procollagen type I C-peptide EIA kit (MK101, Takara, Japan) to convert ng / ml. The amount of collagen synthesized thereby was measured.

division Concentration (µg / ml) 10 100 No treatment 40.3 Example 1 59.4 80.3 Example 2 66.9 88.9 Comparative Example 1 45.8 49.6 Comparative Example 2 51.0 60.8 Comparative Example 3 48.2 53.5 Comparative Example 4 50.8 62.5

In order to confirm the effect of improving skin wrinkles, the amount of protein collagen to maintain the elasticity of the skin was measured.

As shown in Table 3, the cosmetic composition prepared according to Examples 1 and 2 of the present invention was confirmed to promote the production of type 1 procollagen compared to the composition of the untreated group and Comparative Examples 1 to 4.

In particular, it was confirmed that the cosmetic composition of Example 2 promotes the production of type 1 procollagen as compared to the cosmetic composition of Example 1.

That is, the cosmetic composition prepared according to Examples 1 and 2 of the present invention promotes the production of type 1 procollagen and inhibits the expression of collagen degrading enzyme MMP-1, thereby effectively preventing wrinkle formation, It was confirmed that there is an effect to improve the generated wrinkles.

Test Example 3 Whitening Effect

3-1. Tyrosinase Inhibitory Effect

For the cosmetic compositions of Examples and Comparative Examples, the determination of tyrosinase enzyme activity inhibitory activity, which is a representative method of the whitening efficacy evaluation test, was performed using the dopachrome method as follows.

150 μl mushroom tyrosinase-150 unit, 225 μl 2.5 mM L-tyrosine, 225 μl 0.4 M hepes buffer (pH 6.8); After mixing 300 μl of ethanol solution or sample (0.5, 1 and 5 mg / ml) solution, the absorbance was measured at 475 nm after incubation for 15 minutes and before incubation. Tyrosinase inhibitory activity was calculated by the following equation (3), the results are shown in FIG.

[Equation 2]

      Tyrosinase inhibition (%) = (C-D)-(A-B) / (C-D) X 100

      (A: absorbance after culturing the sample containing solution, B: absorbance before culturing the sample containing solution, C: absorbance before culturing the sample free solution, D: absorbance after culturing the sample free solution)

division Concentration (µg / ml) 10 100 No treatment 88.7 Example 1 58.4 71.2 Example 2 59.6 77.4 Comparative Example 1 75.6 88.2 Comparative Example 2 66.8 83.5 Comparative Example 3 71.5 86.4 Comparative Example 4 68.7 87.6

As shown in Table 4, the cosmetic composition prepared according to Examples 1 and 2 of the present invention was confirmed that the tyrosinase inhibitory effect is superior to the composition of the untreated group and Comparative Examples 1 to 4.

In particular, it was confirmed that the cosmetic composition of Example 1 is more excellent in tyrosinase inhibitory effect than the cosmetic composition of Example 2.

3-2. Melanin production inhibitory effect

B16F1 cells, which are mouse-derived melanocytes, produce melanin, which is biosynthesized from the melanosomes, which are organelles in the melanocytes in the basal epidermal layer, which can be compared by measuring the absorbance of the visible region.

After inoculating B16F1 cells, the samples were treated for 3 days, the medium was removed, the cells were washed with PBS, 1 ml of 1 N NaOH was added to each well, followed by stirring to melt the cell membranes to dissolve the melanin component. The amount produced was compared by measuring the absorbance at 400 nm, which is the shortest blue visible light region.

division Concentration (µg / ml) 10 100 No treatment 40.9 Example 1 53.4 61.2 Example 2 66.9 74.8 Comparative Example 1 40.6 43.1 Comparative Example 2 49.4 54.2 Comparative Example 3 45.3 49.9 Comparative Example 4 46.1 50.6

As shown in Table 5, it was confirmed that the cosmetic composition prepared according to Examples 1 and 2 of the present invention showed excellent melanin production inhibitory ability as compared to the composition of the untreated group and Comparative Examples 1 to 4.

In particular, it was confirmed that the cosmetic composition of Example 2 is more excellent melanin production inhibitory effect than the cosmetic composition of Example 1.

Test Example 4 Moisture Sustainability, Non-Bulge Sustainability and Adhesion

Twenty women aged 20 to 40 years were included in the experiment. BB creams were prepared with the cosmetic compositions of Examples and Comparative Examples and then provided to a total of 20 test subjects, and scored for adhesion. In addition, the same test subjects were allowed to alternately use the cosmetic compositions of Examples and Comparative Examples one day, and a questionnaire was conducted for each of the moisture persistence and non-aggregation persistence after 4 days.

As the cosmetic composition of the comparative example, the cosmetic compositions of Comparative Examples 2 and 4, which are effective in wrinkle improvement and whitening, were used in the Comparative Examples.

Moisture persistence

Division (Unit: person (%)) Concentration (µg / ml) 10 100 Example 1 Very good 4 (20%) 5 (25%) good 10 (50%) 10 (50%) usually 6 (30%) 5 (25%) Bad -(0%) -(0%) Very bad -(0%) -(0%) Example 2 Very good 6 (30%) 7 (35%) good 11 (55%) 11 (55%) usually 3 (15%) 2 (10%) Bad -(0%) -(0%) Very bad -(0%) -(0%) Comparative Example 2 Very good -(0%) -(0%) good 3 (15%) 2 (10%) usually 5 (25%) 3 (15%) Bad 10 (50%) 13 (65%) Very bad 2 (10%) 2 (10%) Comparative Example 4 Very good -(0%) -(0%) good 4 (20%) 3 (15%) usually 7 (35%) 6 (30%) Bad 8 (40%) 10 (50%) Very bad 1 (5%) 1 (5%)

As shown in Table 6, the cosmetic composition prepared according to Examples 1 and 2 of the present invention was confirmed that the satisfaction of the moisture persistence is higher than the compositions of Comparative Examples 2 and 4.

Unsustainable Sustainability

Division (Unit: person (%)) Concentration (µg / ml) 10 100 Example 1 Very good 5 (25%) 6 (30%) good 9 (45%) 11 (55%) usually 6 (30%) 3 (15%) Bad -(0%) -(0%) Very bad -(0%) -(0%) Example 2 Very good 7 (35%) 8 (40%) good 10 (50%) 11 (55%) usually 3 (15%) 1 (5%) Bad -(0%) -(0%) Very bad -(0%) -(0%) Comparative Example 2 Very good -(0%) -(0%) good -(0%) 1 (5%) usually 6 (30%) 7 (35%) Bad 11 (55%) 9 (45%) Very bad 3 (15%) 3 (15%) Comparative Example 4 Very good -(0%) -(0%) good 1 (5%) 2 (10%) usually 10 (50%) 12 (60%) Bad 7 (35%) 6 (30%) Very bad 2 (10%) -(0%)

As shown in Table 7, the cosmetic composition prepared according to Examples 1 and 2 of the present invention was confirmed to have a higher satisfaction with the sustainability that does not aggregate for a long time compared to the compositions of Comparative Examples 2 and 4.

Adhesion

Division (Unit: person (%)) Concentration (µg / ml) 10 100 Example 1 Very good 6 (30%) 8 (40%) good 10 (50%) 11 (55%) usually 4 (20%) 1 (5%) Bad -(0%) -(0%) Very bad -(0%) -(0%) Example 2 Very good 8 (40%) 11 (55%) good 10 (50%) 8 (40%) usually 2 (10%) 1 (5%) Bad -(0%) -(0%) Very bad -(0%) -(0%) Comparative Example 2 Very good -(0%) -(0%) good -(0%) -(0%) usually 3 (15%) 2 (10%) Bad 13 (65%) 11 (55%) Very bad 4 (20%) 7 (35%) Comparative Example 4 Very good -(0%) -(0%) good 1 (5%) -(0%) usually 9 (45%) 12 (60%) Bad 10 (50%) 5 (25%) Very bad -(0%) 3 (15%)

As shown in Table 8, the cosmetic composition prepared according to Examples 1 and 2 of the present invention was confirmed that the satisfaction of the adhesion of the cream adheres to the skin is higher than the compositions of Comparative Examples 2 and 4.

An example of preparation of a cosmetic composition suitable for applying the present invention will be presented.

Preparation Example 1: Lotion

Preparation examples of the lotion using the cosmetic composition of Example 2 are shown in Table 9.

ingredient Content (% by weight) Composition of Example 2 5.0 glycerin 6.0 1,3-butylene glycol 3.0 Fiji 1500 1.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaluronate 5.0 ethanol 10.0 Polysorbate 20 0.2 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Production Example  2: lotion

A preparation example of a lotion using the cosmetic composition of Example 2 is shown in Table 10 below.

ingredient Content (% by weight) Composition of Example 2 3.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocopheryl Acetate 3.0 Floating paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxy Vinyl Polymer 1.0 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Production Example  3: bb cream

The preparation example of BB cream using the cosmetic composition of Example 2 is as Table 11 below.

ingredient Content (% by weight) Composition of Example 2 1.0 Lipophilic Monostearate Glycerin 1.5 Cetearyl Alcohol 1.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl isostearate 5.0 Squalane 5.0 Mineral oil 35.0 Dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 Triethanolamine 0.7 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Production Example  4: essence

Preparation of the essence using the cosmetic composition of Example 2 is shown in Table 12 below.

ingredient Content (% by weight) Composition of Example 2 1.5 glycerin 10.0 Betaine 5.0 PEG 1500 2.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl Cellulose 0.1 Sodium hyaluronate 8.0 Carboxy Vinyl Polymer 0.2 Triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeceth-16 0.4 ethanol 6.0 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Production Example  5: mask For pack  latex

The preparation example of the mask pack emulsion using the cosmetic composition of Example 2 is as Table 13 below.

ingredient Content (% by weight) Composition of Example 2 1.0 Polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 Cyclodextrin 0.15 DL-panthenol 0.4 Allantoin 0.1 Glycyrisin Monomonium Ammonium 0.3 Nicotinamide 0.5 ethanol 6.0 PEG 40 Cured Castor Oil 0.3 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Claims (10)

A cosmetic composition having a skin wrinkle improvement effect, a whitening effect and an increase in water retention duration, including a bifidus extract, a star anise extract, fermentation of star anise with bifidus bacterium, a Virginia rhododendron leaf extract, and an Indian gooseberry fruit extract. According to claim 1, wherein the cosmetic composition is a cosmetic composition, characterized in that it further comprises a mixed extract of mulberry bark and sky root. The cosmetic composition according to claim 1, wherein the bifidus extract is bifida ferment lysate, which is an extract obtained by culturing bifidus bacteria and crushing cells with a homogenizer. delete The method of claim 2, wherein the mulberry bark and sky root mixture extract is a cosmetic composition, characterized in that the extract of fermenting the mixture of mulberry bark and sky tree root with bifidus. The cosmetic composition according to claim 2, wherein the mulberry bark and the skyroot are mixed in a weight ratio of 1: 0.2-0.8. The cosmetic composition of claim 2, wherein the mixed extract of the mulberry bark and the sky root is mixed in an amount of 1 to 20 parts by weight based on 100 parts by weight of the bifidus extract. According to claim 1, wherein the cosmetic composition is 5 to 30 parts by weight of star anise extract, 1 to 10 parts by weight of Virginia leaf perennial extract and 10 to 40 parts by weight of Indian gooseberry fruit extract based on 100 parts by weight of bifidus extract Cosmetic composition. According to claim 1, wherein the cosmetic composition is BB cream, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, sunscreen cream, moisturizing cream, hand cream , Foundation, essence, nutrition essence, mask pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and a cosmetic composition, characterized in that it is used in combination with a cosmetic selected from the group consisting of a body cleanser. delete