JP2016074609A - External composition for skin and oral composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an external composition for skin or an oral composition derived from natural products with excellent biosafety and having effects on skin aging prevention, the improvement of alterations in skin color, the improvement of skin health, and inhibition of hair loss.SOLUTION: The invention provides a collagen synthesis promoter, a fibroblast activator, an estrogenic agent, and a proteasomal activity enhancer which contain as an active ingredient the extract of Vigna angularis which is a Fabaceae plant; and an external composition for skin or an oral composition which contain their agents.SELECTED DRAWING: None

Description

  The present invention relates to a functional material obtained from azuki bean, a leguminous plant, and blended in cosmetics (including quasi-drugs) and cosmetic or health oral compositions.

  In the skin, internal factors such as inactivation of cell growth / differentiation with age, decrease in hormone secretion, quantitative decrease in extracellular matrix components, active oxygen induced by sunlight (ultraviolet rays), air pollutants And chemical substances such as environmental hormones, allergens such as pollen, and external factors such as environmental stress are intricately intertwined, resulting in aging, rough skin, and changes in color.

  In recent years, it is known that proteasomes present in cells are also involved in aging of human cells. This proteasome is distributed in both cytoplasm and nucleus in eukaryotic cells, and is a huge enzyme complex that degrades proteins in cells. For example, the proteasome degrades proteins (crosslinked, glycated or carbonylated proteins) that have been degraded by ultraviolet rays or active oxygen, and prevents the degradation proteins from accumulating in the cells, maintaining and improving the function of cells such as skin. It is known to contribute to

  In order to prevent skin aging and keep the skin healthy and youthful, the use of various active ingredients has been proposed in the past, and cosmetics containing these active ingredients (beautifying skin ingredients, whitening ingredients, etc.) are now on the market. Has been. For example, antioxidants such as vitamin C, vitamin E and catalase; anti-inflammatory agents such as glycyrrhizic acid; various ultraviolet absorbers; cell activation such as α-hydroxycarboxylic acid, placenta extract, γ-amino-β-hydroxybutyric acid Agents; extracellular matrix components such as collagen, elastin and hyaluronic acid; and humectants such as urea. Further, arbutin, kojic acid, and the like are known as substances that suppress the occurrence of pigmentation such as skin spots, buckwheat, and melasma, and are widely used as active ingredients for whitening agents.

  As described above, functional materials having a skin-healing effect have been proposed based on various skin aging phenomenon mechanisms, but there are problems in terms of safety and effectiveness with respect to skin and the like. . Therefore, there is a demand for functional materials with improved points.

  As a result of intensive studies in view of the problems of the prior art, the present inventors have found that an extract of azuki bean, a leguminous plant, has an excellent collagen synthesis promoting action, fibroblast activation action, female hormone-like action. And proteasome activity enhancing action, and by interacting with these extracts, it has excellent anti-aging effect, skin healthening effect and whitening effect by blending the extract, and has excellent skin safety. It has been found that it is possible to provide compositions and oral compositions.

  Conventionally, the use of azuki bean or azuki bean-derived saponin as a moisturizer, skin barrier function improving agent has been known by the description of Patent Documents 1 to 4, etc., but the extract of azuki bean promotes collagen synthesis, It has not been known that it has a fibroblast activation action, a female hormone-like action and a proteasome activity enhancement action.

JP 2001-131047 JP 2001-288066 JP 2002-265324 A JP 2003-137768 A

The present invention is a collagen synthesis promoter containing an extract of azuki bean, a leguminous plant, as an active ingredient.
The present invention is a fibroblast activator containing an extract of azuki bean, a leguminous plant, as an active ingredient.
The present invention is a female hormone-like agent containing an extract of azuki bean, a leguminous plant, as an active ingredient.
The present invention is a proteasome activity enhancer containing an extract of azuki bean, a leguminous plant, as an active ingredient.
Moreover, this invention is a skin external composition or oral composition formed by mix | blending the said agent.

The present invention uses an extract of azuki bean, which is a leguminous plant, as an active ingredient, and has the collagen synthesis promoting action, fibroblast activation action, female hormone-like action and proteasome activity enhancing action exhibited by the extract. According to, it can prevent and improve excellent rough skin, wrinkles and tarmi, activate cells, improve skin firmness and gloss, and prevent and improve pigmentation such as spots, freckles and liver spots. An external composition for skin and an oral composition can be provided. Moreover, the composition for external use of skin which has the effect of suppressing hair loss and making the scalp healthy can also be provided.

Hereinafter, preferred embodiments of the present invention will be described in detail.
The present invention relates to a collagen synthesis promoter, a fibroblast activator, a female hormone-like activator, and a proteasome activity enhancer comprising an extract of azuki bean (Vigna angularis or Phaseolus angularis) which is a legaceae (Fabaceae) plant It is. The azuki bean used in the present invention is not limited to a specific variety, and any variety may be used. In addition, in the present invention, as azuki bean extraction site, seeds, whole plants including seeds, or a combination of seeds and other sites (flowers, stems, roots, leaves, etc.) can be used.

  For the preparation of the extract, first, azuki bean (for example, seeds, whole plants including seeds, or a combination of seeds and other parts (flowers, stems, roots, leaves, etc.)) is washed in advance if necessary. After removing the foreign matter, the material is left as it is or dried, and then, if necessary, chopped or pulverized and brought into contact with an extraction solvent for extraction. The extraction method can be performed by contacting with an extraction solvent according to a conventional method such as a dipping method, but a supercritical extraction method or a steam distillation method can also be used.

  As an extraction solvent, water; lower alcohols such as methanol, ethanol, propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin; ethyl acetate, butyl acetate, methyl propionate, etc. Esters; Ketones such as acetone and methyl ethyl ketone; Ethers such as ethyl ether and isopropyl ether; Hydrocarbon solvents such as n-hexane, toluene and chloroform, and the like. These may be used alone or in combination of two or more. Used.

  Among these extraction solvents, water and lower alcohols are used in the present invention from the viewpoint of the effectiveness of the extract obtained, further from the viewpoint of skin irritation, and from the viewpoint that it can be widely applied to cosmetics. Or hydrophilic solvents such as polyhydric alcohols are preferred. Preferable examples of using this hydrophilic solvent include, for example, water, lower alcohols (especially ethanol), or polyhydric alcohols (especially 1,3-butylene glycol) alone, or water and lower alcohols. Examples include the use of a mixed solvent with (especially ethanol) or a mixed solvent of water and a polyhydric alcohol (especially 1,3-butylene glycol, glycerin). Among them, water alone or water with 1,3 -A mixed solvent of butylene glycol is particularly preferred.

  When the mixed solvent is used, for example, if the mixed solvent is water and 1,3-butylene glycol, the volume ratio (hereinafter the same) is 1: 1 to 20: 1, and the mixed solvent is water and ethanol. If it exists, it is preferable to set it as the range of 1: 1 to 20: 1 if it is 1: 1 to 25: 1 and the mixed solvent of water and glycerol.

  Moreover, the weight ratio of the dried part of adzuki beans (for example, seeds, whole plants including seeds, or a combination of seeds and other parts (flowers, stems, roots, leaves, etc.)) and the extraction solvent is preferably 1: 1. It is the range of -1: 50, More preferably, it is the range of 1: 5-1: 20.

  In preparing the extract, the pH is not particularly limited, but it is generally preferably in the range of 4-9. In this sense, if necessary, the extraction solvent is blended with an alkaline adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid. You may adjust so that it may become pH of.

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, water or 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent. The extraction temperature is preferably in the range of 0 ° C to 80 ° C, more preferably in the range of 0 ° C to 20 ° C, and the extraction time is preferably 1 hour to 1 week, more preferably 4 hours. ~ 3 days range.

  Prior to the extraction process of the present invention or in parallel with the extraction process, azuki bean may be hydrolyzed as necessary. This may improve the storage stability of the azuki bean extract and possibly more effectively use the extract as a cosmetic compounding agent.

  In the case of subjecting the extract to an enzymatic hydrolysis treatment, examples of the enzyme include proteolytic enzymes such as actinase, papain and pepsin, starch degrading enzymes such as glucoamylase, α-amylase and β-amylase, cellulase, hemicellulase and pectinase. One or two or more selected from the group of enzymes of lipolytic enzymes such as fibrinolytic enzymes and lipases may be used, but one or more selected from these enzyme groups, respectively. It is more preferable to use a combination of these enzymes.

  The amount of enzyme added is, for example, preferably in the range of 0.01 to 50% by weight, and more preferably in the range of 0.1 to 10% by weight, based on the solid content of the extracted part of azuki bean. .

  In general, the extract prepared as described above may be adjusted to a pH of 4 to 8 and used as it is as a cosmetic compounding agent as it is or as a desired concentration by vacuum concentration or the like. You may do it. In addition, the extract may be dried by a conventional method such as spray drying.

  In addition, the extract prepared as described above may be refrigerated for a certain period of time in order to enhance storage stability and the like, and the supernatant may be used as a cosmetic compounding agent.

  Cosmetics (including quasi-drugs) containing the azuki bean extract of the present invention include, for example, emulsion, cream, lotion, essence, pack, lipstick, foundation, liquid foundation, makeup press powder, cheek red, white powder, Facial cleansers, body shampoos, hair shampoos, soaps, and the like are included, and hair restorers and bath agents are also included, but of course not limited thereto. In addition, as cosmetic oral compositions, beverages such as beauty drinks, energy drinks, sports drinks, near water, vitamin drinks, mineral drinks, alcoholic drinks; various soups (including powdered soups), dairy products, jelly, candy , Tablets, gums and other foods; tablets, liquids, granules or jelly-like health foods / beverages, etc., but the present invention is not limited to these, and can be incorporated into foods and drinks that can be taken orally can do.

  The amount of the extract of the present invention in cosmetics is generally 0.002 to 1.0% by weight (solid content% by weight, the same applies hereinafter), preferably 0 as the solid content of the extract in the case of basic cosmetics. In the case of makeup cosmetics, generally in the range of 0.002 to 1.0% by weight, preferably in the range of 0.02 to 0.2% by weight. The case is generally in the range of 0.002 to 10.0% by weight, preferably 0.02 to 7.0% by weight. In the case of hair cosmetics, the solid content of the extract is generally 0.00001 to 5.0% by weight, preferably 0.0001 to 3.0% by weight. Moreover, the compounding quantity of the extract of this invention in an oral composition has the preferable range of 0.1 to 15 weight% as solid content of an extract.

  In cosmetics, in addition to the extract of azuki bean, an essential ingredient, ingredients usually used in cosmetics such as oily ingredients, surfactants (synthetic and natural products), moisturizers, thickeners, antiseptics and sterilizers An agent, a powder component, an ultraviolet absorber, an antioxidant, a pigment, a fragrance and the like can be appropriately blended as necessary. Moreover, as long as the effectiveness and characteristics of the extract of azuki bean are not impaired, it may be combined with other physiologically active ingredients.

  Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

  Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

Examples of emulsifiers and emulsifiers include stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, and soybean-derived water-soluble Polysaccharides, soy-derived protein and polysaccharide complex, lanolin or derivatives thereof, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.) saponins and derivatives thereof , Lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented cereals (wheat, legumes, millet, etc.) and the like can also be blended.

Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, mucopolysaccharides (for example, hyaluron). Acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen and derivatives thereof, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) Examples include extracts, various amino acids, and derivatives thereof.

Examples of the thickener include brown algae such as alginic acid, agar, carrageenan and fucoidan, green algae or red algae-derived components; silane root (white) extract; pectin, locust bean gum, aloe polysaccharides, alkaligenes-producing polysaccharides, etc. Polysaccharides; gums such as xanthan gum, tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer and acrylic acid / methacrylic acid copolymer; hyaluronic acid And its derivatives; polyglutamic acid and its derivatives.

Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Ethanol derived from plants such as Luconium, Salicylic acid, Ethanol, Undecylenic acid, Phenols, Jamal (Imidazodenyl urea), 1,2-pentanediol, various essential oils, bark dry product, radish fermentation liquor, sugarcane, etc. Examples include 3-butylene glycol.

Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powder, grains (rice, wheat, corn, millet, etc.) powder, beans (soybean, azuki bean, etc.) powder, etc.

Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (for example, vitamin E nicotinate, vitamin E linoleate, etc.).

  Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, ellagic acid and derivatives thereof, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof, 4 -Methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate) , Adenosine monophosphate), and these may be blended singly or in combination.

  Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyltocopherylmaleic acid, ascorbyltocopherylphosphate K, myristyl 3-glyceryl ascorbi Acids, ascorbic acid sugar derivatives such as caprylyl 2-glyceryl ascorbic acid, acylated 6-positions of these ascorbic acid sugar derivatives (acyl group is hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate L-ascorbic acid tetrafatty acid esters such as L-ascorbic acid tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or Acylated derivatives thereof, glycerin derivatives of ascorbic acid such as bisglyceryl ascorbic acid, aminopropyl L-ascorbic acid phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl Examples of hydroquinone derivatives include scorbyl phosphate, arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), and tranexamic acid derivatives such as tranexamic acid esters (for example, tranexam). Acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), amides of tranexamic acid (for example, tranexamic acid methylamide) and the like. Examples of resorcinol derivatives include 4-n-butylresorcinol, Examples of 2,5-dihydroxybenzoic acid derivatives such as 4-isoamylresorcinol include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propyl. Examples of nicotinic acid derivatives include nicotinic acid amide and benzyl nicotinate, and α-hydroxy acids include, for example, lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid. .

  Examples of the physiologically active component include placenta extract, Sakuhakuhi extract, Yukinoshita extract, perilla extract, rice bran extract or hydrolyzate thereof, white coconut extract or hydrolyzate thereof, fermented white coconut, peony Extract or hydrolyzate thereof, lactic acid bacteria fermented rice, murasakixikib extract, lotus seed extract or hydrolyzate thereof, lotus seed fermented product, party extract or hydrolyzate thereof, pearl hydrolyzate, pearl seed fermented product, Royal jelly fermented product, sake lees extract or ceramide contained therein, sake lees fermented product, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract, etc. It is done. Also, coral grass extract, rice leaf extract or hydrolyzate thereof, eggplant (water eggplant, long eggplant, Kamo eggplant, rice eggplant etc.) extract or hydrolyzate thereof, apricot fruit extract, catamen giraffe Extracts of seaweeds such as seaweed, extracts of marine flowering plants such as sea cucumber, fermented soymilk, jellyfish water, rice extract or hydrolyzate thereof, rice fermentation extract, germinated rice extract or hydrolyzate thereof, germinated rice Fermented product, black bean extract or hydrolyzate thereof, damask rose flower extract, bamboo shoot peel extract, linoleic acid and its derivatives or processed products (such as liposomal linoleic acid), animal or fish collagen And derivatives thereof, elastin and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and derivatives thereof, allantoin, dii Propylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, red ginseng extract, beetroot extract, loofah extract, anaosa extract, peach extract, peach extract , Kiwi extract, sunflower extract, Zizyphus joazeiro extract, paudarco bark extract, dairy lily extract or ferment, hibiscus flower extract or ferment, hagoromogusa extract, cherimoya extract, mango Extract, mangosteen extract, funori extract, oolong tea extract, red rich extract, purple orchid extract, yam peel or seed coat extract or hydrolysate, safflower flower extract, casablanca extract, sweet potato extract or fermentation , Guava leaf extract, dokudami extract, evening birch extract, aloe extract, strawberry flower Distillate, there is an apple extract or the like.

  Next, the present invention will be described more specifically with reference to production examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Preparation of extract of adzuki seeds 1200 g of a mixed solvent (volume ratio 1: 1) of purified water and 1,3-butylene glycol was added to 150 g of dried adzuki seeds. After adding the mixed solvent, extraction was performed at 4 ° C. The extract was filtered to obtain 820 g of a pale yellow transparent azuki bean seed extract (solid content concentration 1.1%).

Production Example 2 Preparation of azuki bean whole extract 1200 g of a mixed solvent (volume ratio 1: 1) of purified water and 1,3-butylene glycol was added to 150 g of a dried bean whole plant containing seeds. After adding the mixed solvent, extraction was performed at 4 ° C. The extract was filtered to obtain 780 g of a pale yellow transparent whole plant extract of azuki bean (solid content concentration 0.8%).

Example 1. Lotion [A component] part Olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[B component]
Extract of Production Example 1 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide Appropriate amount Purified water Amount that makes the total amount 100 parts [C component]
Perfume Appropriate A component and B component were each heated to 80 ° C. or higher, then B component was added to A component and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Formulation Example 2 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 2 was used instead of the extract of Production Example 1 contained in the component B of Formulation Example 1.

Formulation Example 3 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[B component]
Extract of Production Example 1 3.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Dipotassium glycyrrhizinate 0.5
Amount of purified water totaling 100 parts
[C component]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Formulation Example 4 Emulsion Emulsion in the same manner as in Formulation Example 3 except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside in Formulation Example 3 Got.

Formulation Example 5 Emulsion In the same manner as in Formulation Example 3, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside, Obtained.

Formulation Example 6 Emulsion In the same manner as in Formulation Example 3, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. An emulsion was obtained.

Formulation Example 7 Emulsion In the same manner as in Formulation Example 3, except that 2.0 parts of chamomile extract is used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the B component of Formulation Example 3. An emulsion was obtained.

Formulation Example 10 Emulsion In the same manner as in Formulation Example 3, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. An emulsion was obtained.

Formulation Example 11 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[B component]
Extract of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Arbutin 3.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts

Formulation Example 12. Lotion [Ingredient] part Extract of Production Example 1 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume Appropriate amount Potassium hydroxide Appropriate amount Purified water An amount that makes the total amount 100 parts The above ingredients were mixed to obtain a lotion.

Formulation Example 13 Lotion A lotion was obtained in the same manner as in Formulation Example 12 except that 10.0 parts of the extract of Production Example 2 was used instead of the extract of Production Example 1 in the ingredients of Formulation Example 12.

Formulation Example 14. Essence [Ingredients] part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Extract of Production Example 1 5.0
Citric acid 0.3
Sodium citrate 0.6
Purified water in an amount of 100 parts Hyaluronic acid was dissolved in purified water, and then the remaining raw materials were sequentially added and dissolved by stirring to obtain a transparent essence.

Formulation Example 15. Essence Essence was obtained in the same manner as in Formulation Example 14 except that Extract 5.0 of Production Example 2 was used instead of the extract of Production Example 1 among the ingredients of Formulation Example 14.

Example 16 Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[B component]
Extract of Production Example 1 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [C component]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Formulation Example 17. Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[B component]
Extract of Production Example 2 5.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Formulation Example 18. Hair restorer [ingredients] part Dipotassium glycyrrhizinate 0.1
Mononitroguaiacol sodium 0.02
Pyridoxine hydrochloride 0.03
l-Menthol 0.8
Japanese cypress raft root extract 0.3
Brown algae extract 0.3
Panax ginseng extract 0.3
Gentian extract 2.0
Extract of Production Example 1 3.5
Trimethylglycine 0.5
Lactic acid 0.2
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Polyoxyethylene hydrogenated castor oil 0.4
L-arginine appropriate amount ethanol 20
The amount in which the total amount of purified water is 100 parts The above ingredients were sufficiently stirred and mixed to obtain a hair restorer.

Formulation Example 19. Hair shampoo [component A] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[B component]
Citric acid 0.1
Extract of Production Example 1 2.0
1,3-butylene glycol 2.0
Purified water Total amount of 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component, stirring is continued and cooled to room temperature to obtain a hair shampoo It was.

Example 20. Hair conditioner [Component A] Part Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
[Component B] Part Extract of Production Example 1 2.0
1,3-butylene glycol 5.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component, and stirring is continued to cool to room temperature to obtain a hair conditioner It was.

Formulation Example 21. Beverage [Ingredients] part Extract of Production Example 1 10.0
Collagen 8.0
Citric acid 0.1
Sweetener (sucrose) 0.01
Antioxidant (Vitamin C) 0.01
Amount of purified water totaling 100 parts

Formulation Example 22. Tablet [Ingredient] part Extract of Production Example 2 20.0
Vitamin C 20.0
Fatty acid ester 10.0
Calcium lactate 20.0
Lactose 30.0
After the above-mentioned parts by weight of each component were mixed, they were pressure-molded to obtain tablets.

Test Example 1 Evaluation test of collagen synthesis promotion effect Human dermis-derived fibroblasts NB1RGB are seeded at 1 × 10 ⁴ cells / hole in a 96-well microplate containing 0.5% NCS-containing Eagle's minimum essential medium, 37 ° C., 5.0 After pre-culturing for 1 day under the condition of% CO ₂ , the extract of Production Example 1 was added as a sample solution. The sample solution was added so that the extract of Production Example 1 had a final concentration of 1.0% and 2.0% as a solution with respect to the total amount of the medium. After adding the sample solution, the cells were further cultured for 5 days under the same conditions as the preculture. Next, after removing the medium and fixing the cells with cold methanol and cold ethanol, staining was performed with a saturated picric acid aqueous solution containing 0.1% sirius red. After washing with purified water, extraction was performed with a 0.1% NaOH: methanol = 1: 1 solution, and the amount of collagen was measured at a wavelength of 540 nm using a microplate reader (Model 680, manufactured by Bio-Rad). For the control (Control) to which the same concentration of 1,3-butylene glycol was added instead of the sample solution, the same operation as above was performed, and the relative value of the amount of collagen at the time of each sample addition to the amount of collagen obtained here was determined. The fibroblast collagen synthesis rate (%) was obtained. Moreover, in order to confirm whether the test system is functioning normally, the same test was also performed when 1 mM magnesium ascorbate phosphate (APM) was added as a positive control instead of the sample solution.

The results of Test Example 1 are shown in Table 1.
[Table 1]

As shown in Table 1, it was confirmed that the extract according to Production Example 1 of the present invention has an excellent collagen synthesis promoting action in a concentration-dependent manner.

Test Example 2 Evaluation test of fibroblast activation effect Human dermis-derived fibroblasts NB1RGB were seeded at 1 × 10 ⁴ cells / hole in a 96-well microplate containing 0.5% NCS-containing Eagle's minimum essential medium, 37 ° C., 5. After pre-culturing for 1 day under the condition of 0% CO ₂ , the extract of Production Example 1 was added as a sample solution. The sample solution was added so that the extract of Production Example 1 had a final concentration of 1.0% and 2.0% as a solution with respect to the total amount of the medium. After adding the sample solution, the cells were further cultured for 3 days under the same conditions as the preculture. Next, the medium was removed, 0.03% MTT was added, and the mixture was kept at 37 ° C. for 1 hour, and the produced formazan was extracted with isopropanol, and a microplate reader (Model 680, manufactured by Bio-Rad) was used. The MTT value was measured at a wavelength of 570-630 nm. The same operation as described above was performed for the control to which the same concentration of 1,3-butylene glycol was added instead of the sample solution, and the relative value of the MTT value at the time of each sample addition to the MTT value obtained here was calculated. The fibroblast MTT activity rate (%) was determined. Moreover, in order to confirm whether the test system is functioning normally, the same test was also performed when 100 mM glucose was added as a positive control instead of the sample solution.

The results of Test Example 2 are shown in Table 2.
[Table 2]

As shown in Table 2, it was confirmed that the extract according to Production Example 1 of the present invention has an excellent fibroblast activation action depending on the concentration.

Test Example 3 Effect of female hormone-like action Human breast adenocarcinoma cells MCF-7 were seeded at 4 × 10 ³ cells / well in a 96-well microplate containing Dulbecco's modified Eagle's minimum essential medium containing 10% FBS (charcoal dextrin treatment) at 37 ° C. Then, after pre-culturing for 1 day under the condition of 5.0% CO ₂ , the extract of Production Example 1 was added as a sample solution. The sample solution was added so that the extract of Production Example 1 had a final concentration of 1.0% and 2.0% as a solution with respect to the total amount of the medium. After adding the sample solution, culturing for 5 days, discarding the supernatant, washing once with PBS (−), adding 100 μl / well of hoechst33342 reagent diluted 100 times with PBS (−), and then at 37 ° C. for 1 hour Incubate and fluorescently stain the DNA. Thereafter, the fluorescence intensity (excitation: 355 nm, emission: 460 nm: fluorescence microplate reader (Fluoroscan Ascent, manufactured by Thermo Fisher Scientific)) was measured, and the amount of DNA was determined. The group obtained by adding the same concentration of 1,3-butylene glycol instead of the sample solution of Production Example 1 was used as a control, and the fluorescence intensity (DNA amount) obtained here was controlled. The relative value of the fluorescence intensity of each sample addition group with respect to () was determined and used as the cell growth rate (%). Moreover, in order to confirm whether the test system is functioning normally, the same test was also performed when 0.1 nM estradiol was added as a positive control instead of the sample solution.

The results of Test Example 3 are shown in Table 3.
[Table 3]

As shown in Table 3, it was confirmed that the extract according to Production Example 1 of the present invention has a remarkable female hormone-like effect.

Test Example 4 Effect of enhanced proteasome activity Human dermis-derived fibroblasts NB1RGB are seeded at 1 × 10 ⁴ cells / hole in a 96-well microplate containing 0.5% NCS-containing Eagle's minimum essential medium, and 37 ° C., 5.0% CO 2 After pre-culturing for 1 day under the conditions of ₂ , the extract of Production Example 1 was added as a sample solution. The sample solution was added so that the extract of Production Example 1 had a final concentration of 1.0% and 2.0% as a solution with respect to the total amount of the medium. After adding the sample solution, the cells were further cultured for 3 days under the same conditions as the preculture. Thereafter, the cell culture supernatant of each test section was removed, and 100 μL of cell lysate (Tris-HCL buffer (pH 8.0) containing 1% Tween 20: 12 mM trishydroxymethylaminomethane, 5 mM EDTA, 150 mM NaCl, hydrochloric acid) was added. A crude enzyme solution was obtained by disrupting the cells. 50 μL of the crude enzyme solution was placed on a separate plate, and 10 μL of a proteasome activity inhibitory solution (a cell lysate containing 2 mM epigallocatechin gallate) was added thereto, which was used for non-proteasome activity measurement. In addition, 10 μL of cell lysate was added to the remaining crude enzyme solution to adjust the volume, and this was used for measuring the total substrate resolution. To each plate, 10 μL of a buffer containing 65 μM Suc-Leu-Leu-Val-Tyr-AMC as a substrate was added and reacted at 37 ° C. for 1 hour. After completion of the reaction, fluorescence intensity (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) was measured using a fluorescence plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems). The value obtained by subtracting the non-proteasome activity measurement result from the obtained total substrate resolution measurement result was defined as the proteasome activity. In the case of adding no sample (control: Control) in which PBS (−) was added instead of the sample solution of Production Example 1, the same operation as described above was performed, and the proteasome at the time of adding each sample to the proteasome activity obtained here The relative value of the activity was determined and used as the proteasome activity rate (%).

The results of Test Example 4 are shown in Table 4.
[Table 4]

As shown in Table 4, it was confirmed that the extract according to Production Example 1 of the present invention has a significantly superior proteasome activity enhancing effect.

Claims (6)

A collagen synthesis promoter containing an extract of azuki bean, a leguminous plant, as an active ingredient. A fibroblast activator containing an extract of azuki bean, a leguminous plant, as an active ingredient. A female hormone-like agent containing as an active ingredient an extract of azuki bean, a leguminous plant. A proteasome activity enhancer containing an extract of azuki bean, a leguminous plant, as an active ingredient. An external composition for skin comprising the agent according to any one of claims 1 to 4. The oral composition containing the agent of any one of Claims 1-4.