JP2008260721A - External preparation for skin - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an external preparation for the skin, containing an extract of propolis containing isoflavone, having each activity of collagen production-promoting activity, melanin production-inhibiting activity and skin turning over-promoting activity, also having safety and hardly providing skin irritation. <P>SOLUTION: The external preparation for the skin contains the extract of the propolis containing the isoflavone, and obtained from Dalbergia ecastophyllum(L) Taub. classified in the genus Dalbergia of Leguminosae as an original plant. The external preparation for the skin is preferably a cosmetic. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a novel skin external preparation containing an extract of propolis that prevents or ameliorates skin symptoms such as wrinkles and tarmi caused by aging, exposure to ultraviolet rays, stress, and the like.

  Propolis is a resinous substance that is produced by mixing its own secretions with pollen, sap, and other substances collected from plants by honeybees, and is known as one of the materials that make up bees' nests. Flavonoids, minerals, amino acids, vitamins, beeswax, aromatic oils, organic acids, etc., and are broadly classified into poplar propolis and eucalyptus propolis depending on the plant of origin. Ingredients contained in propolis differ depending on the type of plant, but all of them are immune stimulating, antioxidant, anti-aging, anti-inflammatory, antitumor, antibacterial, and antiviral due to the physiological effects of flavonoids. It is said that there is an analgesic action. There have been proposals regarding propolis extracts, and water-soluble propolis powder with an increased flavonoid content (see Patent Document 1) and flavonoid content, while reducing the amount of lipid and other polymer insolubles There is a propolis agent (see Patent Document 2).

By the way, mechanisms that cause skin symptoms such as wrinkles, talmi, and skin keratinization due to aging, UV exposure, stress, etc. are, for example, functional deterioration due to damage of fibroblast genes caused by UV rays and fibrous properties such as collagen and elastin. It has been elucidated that it increases the mRNAw of the enzyme MMPs that cleave proteins, resulting in the reduction or degradation of matrix fibers. In addition, the occurrence of spots and freckles increases the melanin synthesis of pigment cells and increases the number of cells due to UV stimulation, and the pigmentation becomes darker due to keratinocytes actively taking in melanosomes, and the skin due to aging This occurs in conjunction with poor turnover homeostasis. Researches on external skin preparations effective for such skin symptoms have been conducted energetically in various directions, and many effective materials have been reported. For example, in recent years, coenzyme Q10 has been attracting attention in various fields, such as extracts derived from animals, components in plants, and components derived from microorganisms. There is also a proposal of a cosmetic containing an extract of propolis, such as a cosmetic containing a propolis-containing composition (see Patent Document 3) and a cosmetic containing a propolis extract and phenoxyethanol (see Patent Document 4). . In addition, isoflavones, a kind of flavonoid, have a female hormone-like action and have the property of binding strongly to the β-type receptor (ERβ) among the estrogen receptors distributed in the skin, bones, blood vessels, prostate, etc. In addition to the tyrosinase inhibitory action, the suppression of melanin production in the skin, the wrinkle and texture improving action by promoting the production of collagen fibers and hyaluronic acid, it has been elucidated that the sebaceous gland size is reduced by repeated application to the skin.
JP-A-10-179057 (Claim 4, [0017] Table) JP-A-8-214798 (Claim 3, [0034] Table 2) JP-A-9-67243 (Claims 1, [0004], [0012]) Japanese Patent Laid-Open No. 11-302147 (Claims 1, [0003] and [0024])

  However, all of the flavonoids contained in the propolis described in Patent Document 1 and Patent Document 2 relate to a kind of flavonoid flavonol or flavone, and no extract of propolis containing isoflavone has been known so far. The cosmetics described in Patent Document 3 relate to cosmetics useful for prevention of dandruff, hair growth and the like, and the cosmetics described in Patent Document 3 relate to cosmetics having sufficient antiseptic effect and safety. There is no known external preparation for skin containing an extract of propolis effective for skin symptoms such as wrinkles, tarmi, keratinization of the skin, spots and buckwheat. Further, the conventional propolis extract has a problem that it is irritating to the skin and is difficult to be applied externally.

  The present invention has been made in view of the above circumstances, and includes isoflavones, has a collagen production promoting action, a melanin production inhibiting action, and a skin turnover promoting action, has safety, and has no skin irritation. An object of the present invention is to provide a skin external preparation containing a propolis extract.

  As a result of searching for propolis originating from Dalbergia ecastophyllum (L) Taub. Focusing on the physiological activity of isoflavones, the present inventors completed the present invention. That is, the invention described in claim 1 is characterized in that it is derived from Dalbergia ecastophyllum (L) Taub., Which is classified into the genus Leguminosae, and contains an extract of propolis containing isoflavones. The topic is a topical skin preparation. In the present invention, the extract may be extracted with a mixed solvent of ethanol and water.

  The skin external preparation may be a cosmetic. The cosmetic may be a whitening agent, a skin conditioner or an activator.

  The topical skin preparation may be a quasi-drug.

  The above external preparation for skin may be an external medicine. The external medicine may have a skin turnover promoting action.

  The external preparation for skin of the present invention has collagen production promoting action, melanin production inhibiting action, skin turnover promoting action, and has safety and no skin irritation. This improves skin firmness and freshness, and can prevent and improve wrinkles, tarmi, dry skin, rough skin and sunburn, and is useful as a cosmetic, quasi-drug or topical medicine.

  The extract of propolis contained in the topical skin preparation of the present invention is derived from Dalbergia ecastophyllum (L) Taub., Which is classified into the genus Leguminosae, and the propolis containing the sap of the origin plant is used. It can be obtained by extraction. This propolis is produced by collecting bees from the sap of Dalbergia ecastophyllum (L) Taub. Distributed in the state of Aragoas, etc. in northeastern Brazil. Even though propolis is usually brown or dark yellow On the other hand, since it is red, it is commonly referred to as red propolis. The propolis extracted in the present invention is not limited to the above-mentioned Brazilian production, as long as the bees are produced by collecting sap of Dalbergia ecastophyllum (L) Taub.

  Production of the propolis extract is carried out by crushing the above-mentioned propolis bulk to obtain a ground crushed product, which is extracted with an extraction solvent. The extraction solvent is not particularly limited as long as isoflavone can be extracted as an extraction component, but organic solvent, water, mixed solvent of organic solvent and water, vegetable oil such as rice oil and rapeseed oil, synthetic oil such as myristic sanoctyldodecyl, or These mixed solvents can be exemplified. As the organic solvent, lower alcohols such as methanol, ethanol and isopropyl alcohol, polyols such as propylene glycol and butylene glycol, and a mixed solvent of these alcohols and water can also be used. Among these, a mixed solvent of ethanol and water is preferable, and the water ratio is preferably 5% by volume to 90% by volume, and more preferably 30% by volume to 60% by volume. When the water ratio is less than 5% by volume, resin, wax, colored substances, off-flavor substances, etc. are dissolved and contained. When the water ratio exceeds 90% by volume, the isoflavone extraction efficiency is lowered. The extraction solvent is preferably 2 parts by weight or more and 7 parts by weight or less with respect to 1 part by weight of the pulverized propolis mass. If the amount is less than 2 parts by weight, the extraction efficiency decreases, and if it exceeds 7 parts by weight, components other than physiologically active components such as isoflavones of propolis may be eluted. The temperature during extraction is preferably 20 ° C. or higher and 70 ° C. or lower. When the temperature is lower than 20 ° C, the extraction efficiency decreases. When the temperature exceeds 70 ° C, the organic solvent in the extraction solvent volatilizes, which may affect the extraction efficiency or decrease the active ingredient.

  Subsequently, the propolis extract obtained above is preferably subjected to solid-liquid separation by centrifugation, filtration, a combination thereof or the like. The extract of propolis can be used as an extract as it is for a topical skin preparation, but it may be concentrated by reduced pressure, ultrafiltration, etc., or powdered by a vacuum freeze dryer or spray dryer. .

  The propolis extract according to the present invention contains isoflavones. Isoflavones have a higher bioavailability in the aglycone type than in the glycoside type, and there is a report that this tendency is the same in the case of transdermal absorption. Therefore, isoflavones contained in propolis are Most of them are aglycone-type because they pass through metabolism in the plant, and a high utilization rate can be expected. The skin external preparation of the present invention contains isoflavones and thus has a collagen production promoting action, a melanin production inhibiting action, and a skin turnover promoting action. By these actions, skin firmness and freshness due to whitening effect and improvement of skin moisturizing power can be obtained, and wrinkles, tarmi, dry skin, rough skin and sunburn can be prevented and improved. Therefore, the skin external preparation of the present invention can be used as a quasi-drug of makeup cosmetics, hair cosmetics, toiletry products, bath preparations and medicinal cosmetics as well as basic cosmetics. In particular, it is useful as a whitening agent, skin conditioner or activator as a basic cosmetic. Moreover, since it has an action of promoting skin turnover, it can also be used as an external medicine used for skin symptoms such as burns that have damaged the skin.

  Cosmetics to which the external preparation for skin of the present invention can be used include face wash such as lotion, milky lotion, beauty essence, general cream, cleansing cream, pack, shaving cream, sun cream, sunscreen cream, sunscreen lotion, suntan It can be used for various cosmetics such as lotions, cosmetic soaps, foundations, funny, powders, lipsticks, lip balms, eyeliners, eye creams, eye shadows, mascaras, bath cosmetics, shampoos, rinses, hair dyes, hair cosmetics and the like. The blending amount of the cosmetic composition may be 0.001 to 100% by mass (stock solution), but is preferably 0.01 to 10% by mass.

  The skin external preparation of the present invention can be arbitrarily selected without particular limitation from among bases, drugs, or additives that are usually used in cosmetics, quasi drugs, and pharmaceutical skin external preparations. It can be blended and manufactured. For example, protein and protein hydrolysis such as collagen, collagen hydrolyzate, gelatin, gelatin hydrolyzate, elastin, elastin hydrolyzate, lactoferrin, keratin, keratin hydrolysate, casein, albumin, royal jelly derived protein hydrolysate The thing can be illustrated. Hereinafter, bases, drugs, additives and the like that can be blended are exemplified.

  Natural polymers such as sodium hyaluronate, xanthan gum, carrageenan, guar gum, alginic acid and salts thereof, pectin, chondroitin sulfate and salts thereof, water-soluble chitin, chitosan derivatives and salts thereof, deoxyribonucleic acid, gum arabic and tragacanth gum and derivatives thereof.

  Plant extracts such as licorice extract, aloe extract, chamomile extract, perilla extract. Microbial substances such as yeast extract. Seaweed powder and seaweed extract. Animal origin such as placenta extract. Among carboxyvinyl polymers and salts thereof, polyacrylic acid and salts thereof, acidic polymers such as carboxymethyl cellulose and salts thereof, polyvinyl alcohol, hydroxyethyl cellulose, hydroxypropyl cellulose, methyl cellulose, polyethylene glycol, polyvinyl pyrrolidone, nitrocellulose polyvinyl methyl ether, etc. Polymer.

  Cationic polymers such as cationized cellulose, polyethyleneimine, and cationized guar gum. Lower alcohols such as ethanol and isopropyl alcohol. UV absorbers such as paraaminobenzoic acid UV absorbers, salicylic acid UV absorbers, cinnamic acid UV absorbers, anthraniere acid UV absorbers, and benzophenone UV absorbers.

  Vitamin A, vitamin B group, vitamin C, L-ascorbyl magnesium phosphate and derivatives thereof, vitamin D group, acetic acid d-α-tocophenol, vitamin E group, folic acid, β-carotene, γ-oryzanol, nicotinic acid, Vitamins such as pantothenic acids, biotins and ferulic acid.

  Anti-inflammatory agents such as glycyrrhizic acid and its salts, guaiazulene and its derivatives, and allantoin. Antioxidants such as stearic acid ester, nordihydrogua ceretenoic acid, dibutylhydroxytoluene, butylhydroxyanisole, parahydroxyanisole, gallic propyl, sesamol, sesamolin, gossypol.

  Preservatives such as parabenzoates such as methyl parabenzoate, ethyl parabenzoate, propyl parabenzoate and butyl parabenzoate, sorbic acid, dehydroacetic acid, phenoxyethanol and benzoic acid.

  Metal ion sequestering agents such as edetic acid and edetic acid and its salts, phytic acid, hydroxyethanedisulfonic acid and the like.

Polyhydric alcohols such as glycerin, 1,3-butylene glycol and propylene glycol. Amino acids such as L-aspartic acid, DL-alanine, L-arginine, L-cysteine, L-glutamic acid, glycine, and salts thereof.

  Sugars such as maltitol, sorbitol, xylobiose, N-acetyl-D-glucosamine, and honey. Organic acids such as citric acid, lactic acid, α-hydroxyacetic acid, pyrrolidone carboxylic acid, and salts thereof.

  Bases such as aqueous ammonia, monoethanolamine, triethanolamine, sodium hydroxide, potassium hydroxide. Hydrocarbons such as liquid paraffin, squalane and petrolatum.

  Fats and oils such as olive oil, palm oil, evening primrose oil, jojoba oil, castor oil, and hardened castor oil. Fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, and behenic acid.

  Higher alcohols such as myristyl alcohol, cetanol, cetostearyl alcohol, stearyl alcohol, and behenyl alcohol; Esters such as isopropyl myristate, isopropyl palmitate, cetyl octanoate, glyceryl trioctanoate, octyldodecyl myristate, octyl stearate and stearyl stearate. Phospholipids such as lecithin and its derivatives.

  Animal and plant-derived lipids such as bovine bone marrow fat and bovine brain lipid. Alkyl sulfates such as ammonium lauryl sulfate, ethanolamine lauryl sulfate, sodium lauryl sulfate, and triethanolamine lauryl sulfate;

  Polyoxyethylene (2EO) lauryl ether triethanolamine sulfate (EO is ethylene oxide, the number before EO indicates the number of moles of ethylene oxide added), polyoxyethylene (3EO) alkyl (carbon number 11-15) Or a mixture of two or more thereof) polyoxyethylene alkyl sulfates such as sodium ether sulfate.

  Alkylbenzenesulfonates such as sodium laurylbenzenesulfonate and triethanolamine laurylbenzenesulfonate.

  Polyoxyethylene alkyl ether sulfates such as sodium polyoxyethylene (3EO) tridecyl ether acetate.

  Coconut oil fatty acid sarcosine sodium, lauroyl sarcosine triethanolamine, sodium lauroylmethyl-L-glutamate, coconut oil fatty acid-sodium L-glutamate, coconut oil fatty acid-L-glutamate triethanolamine. N-acyl amino acid salts such as sodium coconut oil fatty acid methyl taurine sodium and sodium lauroyl methyl taurate, ether sulfate sodium alkane sulfonate, hydrogenated coconut oil fatty acid sodium glyceryl sulfate, hydrogenated coconut oil fatty acid sodium glycerin sulfate, undecinoylamido ethyl sulfosuccinate Sodium, sodium octylphenoxy dientoxyethyl sulfonate, disodium aminosulfosuccinate, dioctyl sodium sulfosuccinate, disodium lauryl sulfosuccinate, polyoxyethylene alkyl (carbon chain 12-15) ether phosphate (8-10EO), Polyoxyethylene oleyl ether sodium phosphate, polyoxyethylene cetyl ether sodium phosphate, polyoxyethylene sulfosuccinate lauryl dinato Um, sodium polyoxyethylene lauryl ether phosphate, sodium lauryl sulfoacetate, anionic surfactants such as sodium tetradecene sulfonate.

  Stearyldimethylammonium chloride, di (polyoxyethylene) oleylmethylammonium chloride, stearyldimethylbenzylammonium chloride, stearyltrimethylammonium chloride, tri (polyoxyethylene) stearylammonium chloride, polyoxypropylenemethyldiethylammonium chloride, myristyldimethylbenzylammonium chloride Cationic surfactants such as lauryltrimethylammonium chloride.

  2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, sodium undecylhydroxyethylimidazolinium betaine, undecyl-N-hydroxyethyl-N-carboxymethylimidazolinium betaine, stearyl dihydroxyethyl betaine, palm Oil fatty acid amidopropyl betaine, coconut oil alkyl-N-carboxyethyl-N-hydroxyethyl imidazolinium betaine sodium, coconut oil alkyl-N-carboxymethoxyethyl-N-carboxymethylimidazolinium disodium lauryl sulfate, N-coconut Amphoteric surfactants such as oil fatty acid acyl-L-arginine ethyl-DL-pyrrolidone carboxylate.

  Polyoxyethylene alkyl (carbon number 12-14) ether (7EO), polyoxyethylene octyl phenyl ether, polyoxyethylene oleyl ether, polyoxyethylene oleic acid glycerin, polyoxyethylene stearyl ether, polyoxyethylene cetyl ether, polyoxy Ethylene cetyl stearyl diether, polyoxyethylene sorbitol lanolin (40EO), polyoxyethylene nonylphenyl ether, polyoxyethylene polyoxypropylene cetyl ether, polyoxyethylene polyoxypropylene decyl tetradecyl ether, polyoxyethylene lanolin, polyoxy Nonionic surfactants such as ethylene lanolin alcohol and polyoxypropylene stearyl ether.

  Isostearic acid diethanolamide, undecylenic acid monoethanolamide, oleic acid diethanolamide, beef tallow fatty acid monoethanolamide, hardened tallow fatty acid diethanolamide, stearic acid diethanolamide, stearic acid diethylaminoethylamide, stearic acid monoethanolamide, myristic acid diethanolamide, Thickeners such as coconut oil fatty acid diethanolamide, coconut oil fatty acid ethanolamide, lauric acid isopropanolamide, lauric acid ethanolamide, lauric acid diethanolamide, and lanolin fatty acid diethanolamide.

  Silicon oils such as linear or cyclic methylpolysiloxane, methylphenylpolysiloxane, dimethylpolysiloxane polyethylene glycol copolymer, dimethylpolysiloxane polypropylene copolymer, amino-modified silicone oil, and quaternary ammonium-modified silicone oil. Thioglycolic acid and its salts. Cysteamine and its salts. Peroxides such as hydrogen peroxide, persulfate, perborate and urea peroxide. Bromates such as sodium bromate and potassium bromate.

  Others, pH adjusters, colorants, fragrances, stabilizers, refreshing agents, blood flow promoters, keratolytic agents, astringents, wound treatment agents, foam boosters, oral preparations, deodorants / deodorants, antiallergic agents It can also mix | blend with an agent and a cell activator.

  EXAMPLES Next, although an Example is given and this invention is demonstrated, this invention is not limited to a following example.

[Example 1] (Production of propolis extract)
100 g of the propolis bulk was coarsely crushed and immersed in 600 ml of a mixture of ethanol and purified water 1: 1, followed by heating under reflux for about 1 hour in an Erlenmeyer flask equipped with a reflux condenser. After cooling, solid-liquid separation was performed using a high-speed centrifuge, and the supernatant was recovered. Further, the supernatant was filtered using a 0.45 μm membrane filter to obtain a clear extract (extract). The dry powder was produced using the extract using a vacuum freeze dryer.

[Example 2] (Quantification of isoflavones in propolis extract)
2 ml of the propolis extract obtained in Example 1 was taken and made up to 50 ml with methanol. Next, this was filtered through a 0.45 μm membrane filter and quantified by HPLC.
The operating conditions for HPLC are shown below and in Table 1. The results of HPLC are shown in Table 2 and FIG. Table 2 shows the amount of isoflavone per 100 g of propolis extract.
<HPLC operating conditions>
Column: Develosil ODS-HG-5 (4.6 × 250mm, 5μm)
Flow rate: 1mL / min
Analysis time: 45min
Injection volume: 10μL
Detector: Jasco UV-2075 Plus
UV: 268nm
Pump: Jasco PU-2089 Plus
Column oven: Jasco Co-2060 Plus
Mobile phase conditions: gradient according to Table 1
A: Methanol: Water: Acetic acid = 30: 70: 1 (v / v / g)
B: Methanol: Water: Acetic acid = 90: 10: 1 (v / v / g)

  As is clear from Table 2 and FIG. 1, the propolis extract contained the isoflavone formononetin and biocanin A.

[Example 3] (Acute oral administration toxicity test of propolis extract)
The propolis extract obtained above was administered to Sprague Dawley (Sam: TacN SDBR) male and female rats by single acute oral administration, the toxicity was observed, and the approximate lethal dose was examined. The test substance was administered at a dose of 2000 ml / kg, and 2000 ml / kg of water for injection was administered to the control group.

  As a result of the above, there were no deaths in any of the male and female groups in the test substance administration group and the control group throughout the observation period, and no change in general condition was observed. In both groups, a steady increase in body weight was observed in all cases. In the planned necropsy of surviving animals, no abnormalities were observed in all male and female cases in the test substance-administered group and the control group. From this result, in a single oral dose toxicity test of propolis extract in rats, no signs of toxicity considered to be due to the test substance were observed, the approximate lethal dose was 2000 ml / kg or more for both males and females, and the propolis extract was The safety was judged to be high.

[Example 4] (Primary skin irritation test of propolis extract)
Three female Japanese white rabbits weighing approximately 2.8 kg (20 weeks of age) were used, and on the day before the test, the dorsal skin was cut with a clipper for animals in an area of 12 × 12 cm across the midline. The cocoon was fixed, and a propolis extract was dropped into a 2 × 2 cm square at a rate of 0.03 ml per site. Judgment was made at 24, 48, and 72 hours after application by the primary irritation scoring method using erythema and edema as indices.

  As a result of the above determination, no erythema or edema was observed as a result of determination over 24, 48, and 72 hours after application of the propolis extract. Therefore, the primary skin irritation test of the propolis extract was judged negative.

[Example 5] (Examination of collagen production promoting action of propolis extract)
The collagen production effect of the propolis extract was examined by ELISA using normal human fibroblasts W1-38.

  W1-38 cells suspended in DMEM medium supplemented with 10% FBS were cultured until they became confluent (48 hours). Discard the medium and wash the cells three times with PBS. To the cells, add DMEM medium (without FBS) with a pre-concentrated lyophilized powder of propolis extract added, and incubate for 48 hours. Determine the amount of collagen distributed in the medium. Measurement was performed using TAKARA Procollagenn type IC-Peptide (PIP) EIA Kit (Takara Bio Inc.). The control was a dry powder-free section of propolis extract. The results are shown in FIG.

  In FIG. 2, the amount of collagen produced was larger in the group to which the propolis extract was added than in the control group, and the amount of collagen produced increased in proportion to the concentration. When the propolis extract was at a concentration of 1000 ppm, the control ratio exceeded 200%. Therefore, it became clear that the extract of propolis promotes collagen production of fibroblasts.

[Example 6] (Examination of melanin production inhibitory action of propolis extract)
The culture was carried out for 7 days in a medium in which a predetermined amount of propolis extract was added to B16 melanoma 4A5 cells. The cultured cells were centrifuged, and the state of the cell pellet was observed from the bottom of the centrifuge tube. The results are shown in FIG.

  As is clear from FIG. 3, the propolis extract suppressed cell blackening in a concentration-dependent manner. Therefore, it was found that the propolis extract has a melanin production inhibitory effect and a whitening effect.

[Example 7] (Examination of skin turnover promoting action (metabolism promoting effect) of propolis extract)
Vaseline containing the fluorescent reagent dansyl chloride was patched to the upper arm for 24 hours, and then the stock solution of propolis extract was applied to the test part twice a day to observe the disappearance of fluorescence intensity of dansyl chloride.

  As a result of the above, the brightness due to the remaining dansyl chloride is lower in the application part of the propolis extract compared to the untreated section, and by applying the propolis extract, skin turnover is promoted, rough skin, dry skin, It has been found that it is possible to reduce stains, stains, etc.

[Example 8] (Preparation and sensory test of lotion)
A composition according to the formulation shown in Table 3 below was stirred at 80 ° C. and then cooled to room temperature to prepare a skin lotion to obtain a product. All of the obtained lotions were stable. Further, a lotion was prepared in the same manner as in Table 3 except that no propolis extract was added, and this was used as a comparative product. This lotion was subjected to a sensory test by five expert panelists. The evaluation was carried out in five grades for the following items. Table 4 shows the average score of five panelists.
(1) Moist feeling of skin 2. Slightly soft 2. Normal 4. Slightly moist 5. Moist (2) Smooth skin Roughness Somewhat rough. Normal 4. Slightly smooth 5. Smooth (3) Sticky skin Sticky 2. Slightly sticky 2. Normal 4. Slightly refreshing 5. Refreshing

  From Table 4, it was found that the example product obtained moisture retention and skin smoothness compared to the comparative product.

[Example 9] (Preparation of lotion and sensory test)
According to the formulation shown in Table 5 below, (4), (7), (8), (9) and (10) are added to purified water and dissolved by heating. Separately, (1), (2), (3) , (5), (6), (11) were heated to 70 ° C. and mixed. This oil phase was added to the previously prepared aqueous layer and emulsified using a homomixer, then degassed and cooled to prepare a lotion, which was used as a product. Further, a lotion was adjusted in the same manner as in Table 5 except that no propolis extract was added, and this was used as a comparative product.

  The evaluation of the panelists was that the implemented product was a lotion that felt more moist than the comparative product.

It is a HPLC chromatogram of the extract of propolis. It is the graph which showed the collagen production of the extract of propolis with ratio (%) with respect to control. It is the figure which observed the state of the cell pellet from the centrifuge tube bottom part.

Claims (9)

  A skin external preparation characterized by containing a propolis extract containing isoflavones, which originates from Dalbergia ecastophyllum (L) Taub.   The skin external preparation according to claim 1, wherein the extract is extracted with a mixed solvent of ethanol and water.   The skin external preparation according to claim 1 or 2, wherein the skin external preparation is a cosmetic.   The skin external preparation according to claim 3, wherein the cosmetic is a whitening agent.   The skin external preparation according to claim 3, wherein the cosmetic is a skin conditioner.   The external preparation for skin according to claim 3, wherein the cosmetic is an activator.   The skin external preparation according to claim 1 or 2, wherein the skin external preparation is a quasi-drug.   The skin external preparation according to claim 1 or 2, wherein the skin external preparation is an external medicine.   The external preparation for skin according to claim 8, wherein the external preparation has an action for promoting skin turnover.

Images