KR101042690B1 - A composition comprising extract of Polystichum ovatopaleaceum var. coraiense H. Christ Sa.Kurata having anti-aging and anti-wrinkle activity - Google Patents

Abstract

The present invention relates to a skin external pharmaceutical composition and cosmetic composition for anti-aging and anti-wrinkle containing an extract of Chondohichomi (Polystichum ovatopaleaceum var. Coraiense (H. Christ) Sa.Kurata) as an active ingredient.

Chamonahichomi extract according to the present invention exhibits a cell protective action, elastase inhibitory effect, MMP-1 gene expression inhibitory effect, skin elasticity improvement effect and skin condition improvement effect ultimately anti-aging and wrinkles It can be usefully used as a composition and cosmetic composition.

Chondohichomi Extract, Cell Protection, Elastase, MMP-1, Collagen, Elastin, Wrinkle Relief, Skin Elasticity Improvement, Anti-Aging

Description

A composition comprising extract of Polystichum ovatopaleaceum var. coraiense (H. Christ) Sa.Kurata having anti-aging and anti-wrinkle activity}

The present invention relates to a composition for anti-aging and anti-wrinkle containing an extract of Chonnahichomi as an active ingredient, more specifically, cell protection, elastase inhibitory activity, matrix metallo-proteinase (MMP) The present invention relates to a pharmaceutical composition for external application of skin, which has anti-aging and anti-wrinkle effect through inhibition of gene expression, skin elasticity improvement and skin condition improvement.

Human skin is the organ that forms the outermost part of the body and is always exposed to the atmosphere, which is directly affected by sunlight, oxygen, bacteria, and various pollutants. In addition, oxygen occupies 21% of the atmosphere, and aerobic organisms on the earth breathe the rich oxygen in the atmosphere to secure energy through electron receptors in the body to maintain life. However, the oxygen, which is absolutely necessary for life support, is due to the physical, chemical, and environmental days of the body, such as enzymes, reduction metabolism, chemicals, pollutants, and photochemical reactions, resulting in superoxide anion radicals (O ₂ ˙-), When converted to highly reactive reactive oxygen species (ROS), such as hydroxy radicals (OH), hydrogen peroxide (H ₂ O ₂ ), or singlet oxygene (1O ₂ ), they react to free radical reactions. It is known to destroy major macromolecules of lipid-containing cells and to cause abnormalities in metabolism by reacting with cellular components and cell membranes (Harman, D., Ann. NY Acade. Sci., 673, pp126-141). , 1992).

In addition, aging refers to all physiological changes in the body that life on earth undergoes over time, and refers to life phenomena caused by a variety of factors depending on individual individuals. According to Harman's free radical theory, there are in vivo antioxidant defense mechanisms against these reactive oxygen species (ROS), but biomolecules such as lipids, proteins, and nucleic acids are oxidatively freed by free radicals. Damage causes an imbalance between oxidation and antioxidants, and oxidation products accumulate with age, causing aging (Harman, D., J. Gerontol., 11, pp 298-300, 1596; Richter et al., Proc. Natl. Acad. Sci. USA, 85, pp6465-6467, 1988; Oliver et al., J. Biol. Chem., 262, pp5488-5491, 1987; Brunk et al., Mutat.Res ., 27 5, 396-403, 1992).

In particular, skin aging is classified into two types: intrinsic aging and photoaging (EB Doris, Cosmetics & Toiletries., 111, pp31-37, 1996). It is a phenomenon that appears naturally with age due to genetic factors, and it is difficult to artificially control it. However, photoaging is a phenomenon caused by external environmental factors, and it can be said that artificial control is relatively easy. Representative factors of exogenous aging are the production of excess reactive oxygen species by ultraviolet rays in the skin exposed to ultraviolet rays and the reduction of antioxidant enzymes and antioxidants such as vitamin E, C, glutathione and ubiquinol. An imbalance is caused. The result is damage to biological components by lipid peroxidation, protein oxidation, proteolytic enzyme activation, chain and abnormal cross-linking and abnormal cross-linking of collagen and elastin, hyaluronic acid chain cutting, promoting melanin production, and DNA oxidation. Skin aging such as decreased elasticity, wrinkles and blemishes, and freckles (Oikarinen, A., Photodermatol.Photoimmunol.Photomed., 7 (1), pp3-4,1990; Yamauchi, M., J. Invest.Dermatol , 97, pp938-941, 1991).

Therefore, when free radicals or free radicals are generated by ultraviolet rays or external environmental factors, and the active membranes or free radicals attack the cell membranes, the skin causes inflammation and the protein or genetic material is destroyed. Skin aging occurs through a series of skin aging mechanisms that form wrinkles and the like. If we can suppress all the stages of skin aging mechanism, we can fundamentally prevent skin aging, but until now, we have not developed such an ideal anti-aging agent and antioxidant which removes free radicals and free radicals produced by external factors. The most active research is to find an inhibitor of elastase, one of the representative enzymes involved in skin aging.

Elastase is known to be the only degrading enzyme that degrades elastin, an insoluble elastic fibrous protein in animal connective tissue that maintains skin elasticity (Wiedow, O., Schroder, JM, EJ Biol. Chem., 265 (5), p14791, 1990). Elastase is overproduced by increasing age or by external skin irritation such as ultraviolet rays. Over-produced elastase excessively breaks down elastin, weakening collagen-to-collage bonds and creating wrinkles on the skin. In particular, after the age of 40, the elasticity of the skin is drastically reduced due to the action of elastase, as the action of the elastase increases with age, the elastin fibers are partially lost or aggregated, and the collagen fibers are reduced. to be. By inhibiting such elastase activity, wrinkles on the skin can be suppressed (Bissett, DL, Photochem. Photobiol., 46, pp 367-378, 1987; Bizot-Foulon V., Godeat G., W. Int. J. Cos. Sci., 17, pp 255-264, 1995).

In addition, MMP (matrix metalloproteinase) is a metalloproteinase having zinc at its active center and is secreted in the form of latent protease (zymogen) in vivo. In order to have enzymatic activity, structural modifications occur, resulting in cleavage and activation of amino terminal sites, and activated MMPs are regulated by inhibitors such as 2-macroglobulin or TIMP (tissue inhibitors of metalloproteinase), and keratinocytes in the skin. Many cells, including keratinocytes and fibroblasts, secrete MMP (Fisher, GJ et al., Photochem. Photobiol. 69, 154, 1999). In one UV irradiation, MMP activity in the skin is increased, and collagen in the skin is significantly disrupted, so that MMPs affect collagen breakdown of the dermis and play an important role in photoaging.

Chonnadohichomi (Scientific name: Polystichum ovatopaleaceum var. Coraiense (H.Christ) Sa.Kurata) is a perennial herb that grows on Jeju Island, and has a length of 20-40 cm in length, with constriction, grooves on the surface, dense scales, and The small scales are 2-4mm long. The lobes are two upper right lobes, the ovate is long oval, 40-100cm long and 15-30cm wide, without narrowing or narrowing at the bottom, with dense scales on the back of the axial axis, and the surface is light green. The back side is light green, and the scales on the back side of the axial axis are ovate lanceolate or lanceolate, and the tip is long like tail. The post is linear, without stem and pointed at the end. A postal egg is an oval long oval, dull but thorny, with thorny or soft toothed edges and a short band (National Species Knowledge System).

On the other hand, there is no example of the prior invention regarding the extract of Cinnamyochomi, and the content of the composition containing Cinnamyochomi extract with antioxidant and anti-wrinkle effect has not been published or known anywhere.

Therefore, the inventors of the present invention have antioxidative and elastase inhibitory effects by scavenging free radicals in relation to natural products and skin aging, and as a result of diligent research efforts to search for substances that help to improve skin wrinkles, From the ultra-mime extract confirmed the excellent antioxidant effect and wrinkle improvement effect was completed the present invention.

Therefore, the main object of the present invention is anti-aging and wrinkles containing the extract of Chinnadohichomi having excellent cell protective action, elastase inhibitory effect, MMP-1 gene expression inhibitory effect, skin elasticity improvement effect and skin condition improvement effect It is to provide an external skin pharmaceutical composition or cosmetic composition for improvement.

According to an aspect of the present invention, the present invention provides an external anti-aging and anti-wrinkle pharmaceutical composition containing an extract of Polyhumhumovavalealeum var.coraiense (H.Christ) Sa.Kurata as an active ingredient. do. The present invention is to search for a substance that is effective in cell metabolism activity, skin elasticity and skin aging, the result of searching for an effective substance among various natural substances of nature, the extract of Mt. And it has been found to exhibit the effect of cell proliferation, anti-wrinkle action, skin elasticity improvement and the like.

In the pharmaceutical composition of the present invention, the true Nachochochomi extract may be contained in 0.01 to 50% by weight based on the total weight of the composition, preferably contained in 0.01 to 20% by weight.

In the pharmaceutical composition of the present invention, the cinnamyochomi extract inhibits elastase activity and inhibits the expression of Matrix metalloproteinase (MMP) -1 to prevent anti-aging and wrinkles. It improves. In the embodiment of the present invention it was confirmed that the excellent elastase inhibitory effect, MMP-1 gene expression inhibitory effect, skin wrinkle improvement and skin elasticity enhancing effect of the extract, there is no problem in safety when applied to the skin. .

In the pharmaceutical composition of the present invention, the true Nachochochomi extract can be extracted using a plant extraction method commonly used in the art, preferably water, lower alcohol having 1 to 4 carbon atoms, propylene glycol, It may be extracted in the form of crude extract by extracting with a solvent selected from the group consisting of butylene glycol and glycerin or a mixed solvent thereof. In addition, the solid extract of the crude extract may be dissolved in purified water and then extracted with any one solvent selected from the group consisting of hexane, ethyl acetate, butanol, and water in the form of a soluble extract.

The extracts include crude extracts of true Nachochomi, polar solvent soluble extracts or nonpolar solvent soluble extracts. The crude extract is water, including purified water, lower alcohols having 1 to 4 carbon atoms or at least one solvent selected from the group consisting of propylene glycol, butylene glycol and glycerin, preferably water, ethanol, methanol, butylene glycol, propylene glycol Or extracts soluble in glycerin.

In addition, the polar solvent soluble extract as defined herein is an extract soluble in water, methanol, butanol or a solvent selected from a mixed solvent thereof, preferably water or butanol, and the nonpolar solvent soluble extract is hexane, chloroform, methylene chloride and acetic acid. Extracts soluble in a solvent selected from ethyl, preferably hexane or ethyl acetate.

In the pharmaceutical composition of the present invention, the external skin pharmaceutical composition may be used in any external skin formulation that can be used in the art, and preferably creams, gels, patches, sprays, ointments, warning agents, lotions, lini It is preferable that the formulation of any one selected from cement, pasta and cataplasma.

According to another aspect of the invention, the present invention provides an anti-aging and anti-wrinkle cosmetic composition containing an extract of Chondohichomi (Polystichum ovatopaleaceum var. Coraiense (H.Christ) Sa.Kurata) as an active ingredient . In the embodiment of the present invention as a result of applying directly to the human skin by combining the extract of true Nachochomi of the present invention, the effect of improving the skin condition due to the apparent skin elasticity and aging was observed. The cosmetic composition of the present invention can be used as a raw material of functional cosmetics because it contains an extract of Chonnahichomi that can increase skin elasticity, have anti-wrinkle effect, and prevent skin aging, as a cell protective action.

In the composition of the present invention, the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence It is preferable that the formulation is any one selected from pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

Hereinafter, the present invention will be described in more detail.

First, the preparation of Chamnachochomi extract comprises at least one solvent selected from the group consisting of lower alcohols having 1 to 4 carbon atoms or propylene glycol and butylene glycol, including purified water as the extractant by slicing Chamonachomi, preferably Is an amount of 1 to 30 times, preferably 5 to 25 times, more preferably 10 to 20 times the volume of the extract available in water, ethanol, methanol, butylene glycol, propylene glycol or glycerin, room temperature or cooling condenser The extraction method of stirring extraction, cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, heating extraction, etc. in the state of preventing the solvent from being evaporated, preferably extraction by stirring extraction or heating extraction, It can be prepared by filtration to deposit for 1 to 15 days at 1 to 10 ℃. After repeating the above process 1 to 7 times, preferably 1 to 3 times, concentrated under reduced pressure and dried to obtain the crude extract of the present invention (crude extract).

In addition, the polar solvent and the non-polar solvent soluble extract of the present invention is dispersed in the water of 2 to 30 times, preferably 8 to 25 times, more preferably 10 to 20 times the volume of the crude extract obtained above. After the addition, using a nonpolar solvent such as hexane, ethyl acetate, chloroform, or a polar solvent such as butanol, ethanol, and the like, the volume is added to the same volume in sequence and fractionated 1 to 5 times, preferably 2 to 4 times, and the true content of the present invention. A soluble extract or a non-polar solvent soluble extract of nadohichomi polar solvent can be obtained.

The skin external pharmaceutical composition or cosmetic composition for anti-aging and wrinkle improvement of the present invention comprises 0.1 to 50% by weight of the crude extract or a soluble extract fractionated therefrom based on the total weight of the composition.

The crude extract of Chonnahichomi or the soluble extract extracted from it showed excellent antioxidant effect, wrinkle improvement effect and skin elasticity effect, and also confirmed that there is no problem in safety when applied to the skin.

The present invention prepared a skin external pharmaceutical composition for anti-aging and anti-wrinkle using the crude extract of Chonnahichomi obtained by the above production method or a soluble extract extracted from it as an active ingredient.

The skin external pharmaceutical composition containing the crude extract of the present invention or a soluble extract extracted from the extract as an active ingredient may further include appropriate carriers, excipients and diluents commonly used in the preparation of pharmaceutical compositions.

The pharmaceutical dosage form of the crude extract of Chamonachochomi of the present invention or the soluble extract extracted from the fraction may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds. But can be used as a suitable assembly.

Carriers, excipients and diluents that may be included in the compositions of the present invention include lactose, dextrose, sucrose, oligosaccharides, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium Silicates, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used.

The composition of the present invention is a skin external preparation formulation which can be applied to the skin, and is prepared as a pharmaceutical composition in the form of an external preparation for skin such as cream, gel, patch, spray, ointment, warning, lotion, linen, pasta or cataplasma. However, the present invention is not limited thereto.

The preferred dosage of the composition of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. Topical administration may be administered once a day or may be divided several times.

In addition, the present invention provides a cosmetic composition containing a crude extract of Myeonnahichomi or a soluble extract extracted from it as an active ingredient.

Extracts of the present invention or fractions fractionated therefrom may be used in various ways, such as cosmetics or face washes having an anti-aging and anti-wrinkle effect. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, skins, and the like, cleansing agents, face washes, soaps, treatments, and essences.

The cosmetic composition of the present invention may include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extracts.

The water-soluble vitamin can be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. And salts thereof (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) can also be used in the present invention. Included in The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl) Ethyl ether) and the like are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound that can be blended into cosmetics, but preferably ceramide, phytosphingosine, sphingosaccharide lipid, and the like. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound that can be formulated in cosmetics, but may preferably include brown algae extract, red algae extract, green algae extract, and the like, and calginane, arginate and sodium arginate purified from these seaweed extracts. , Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

The cosmetic of the present invention may be blended with the above essential components, if necessary, with other ingredients normally blended into the cosmetic.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

Examples of the ester-based oils and fats include glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristin, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid. Butyl acid, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octylate dodecyl, isostearic acid isetyl, diethyl sebacinate, adidi Diisopropyl phosphate, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylolpropane, trimethyl stearate trimethylol propane, tetra 2-ethylhexanopentane Tol, cetyl caprylate, lauric acid decyl, hexyl laurate, myristic acid decyl, myristic acid myristyl, myristic acid cetyl, stearyl stearate, decyl oleate, ricinole Sancetyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid , 2-ethylhexanoate cetostearyl, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid) , Propylene glycol dicaprylic acid, neopentyl glycol dicapric acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl tritridecyl, glyceryl triisopalmitate, glyceryl triisostearate, octyl neopentane Dodecyl, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearic acid isostere Reel, isostearic acid octyldecyl, polyglycerol oleic acid ester, polyglycerol isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauric lactate, myritic lactate, cetyl lactate, octyl lactate , Triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, diethyl 2-ethylhexyl succinate, diisobutyl adipic acid, sebacic acid di Isopropyl, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearic acid, oleate Steyl, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-s Ester type | system | groups, such as the alloyl hydroxy stearic acid isostearyl, etc. are mentioned.

Examples of the hydrocarbon-based oils and fats include hydrocarbon-based oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and vaseline.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane and methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, Alkyl modified silicone oil, amino modified silicone oil, etc. are mentioned.

Perfluoro polyether etc. are mentioned as said fluorine-based fat or oil.

Examples of the animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grapes. Seed oil, cottonseed oil, palm oil, cooing nut oil, wheat germ oil, rice germ oil, shea butter, walnut colostrum, marker demi nut nut, meadow home oil, egg yolk oil, Uji oil, horse oil, mink oil, orange rape oil, Animal or plant fats and oils, such as jojoba oil, canderary wax, carnava wax, liquid lanolin, and hardened castor oil.

As said moisturizer, a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, a fat-soluble polymer, etc. are mentioned.

Examples of the water-soluble low molecular moisturizer include serine, glutamine, sorbitol, mannitol, pyrrolidone sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), poly Propylene glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymers include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be mentioned.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

As said emollient agent, long-chain acyl glutamic acid cholesteryl ester, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, etc. are mentioned.

As said surfactant, a nonionic surfactant, anionic surfactant, cationic surfactant, an amphoteric surfactant, etc. are mentioned.

Examples of the nonionic surfactant include self-emulsifying monoglycerate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE-POP (polyoxyethylene polyoxypropylene) copolymer, POE-POP alkyl ether, polyether modified silicone, laurin Acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

As the anionic surfactant, fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkylnaphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphorus salt, alkyl Amide phosphate, alkyloylalkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate salt, sodium alkyl sulfo acetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. Can be.

Examples of the cationic surfactant include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyl trimethylammonium chloride, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzyl ammonium bromide, behenyltrimethylammonium, Benzalkonium chloride, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of the amphoteric surfactant include a carboxybetaine type, an amidebetaine type, a sulfobetaine type, a hydroxysulfobetaine type, an amide sulfobetaine type, a phosphobetaine type, an aminocarboxylate type, an imidazoline derivative type, an amideamine type, and the like. Amphoteric surfactants; and the like.

Examples of the organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengal, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, and oxide Inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, coloramine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As said organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

As said ultraviolet absorber, paraamino benzoic acid, ethyl paraamino benzoate, amyl paraamino benzoate, octyl paraamino benzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid, and cinnamic acid Benzyl, paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, Urocanoic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenonedisulfonic acid sodium salt, dihydroxybenzo Phenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2'-ethylhexyl-1'-oxy)- 1,3,5-triazine, 2- (2- And the like can be de-hydroxy-5-methylphenyl) benzotriazole.

Examples of the fungicides include hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcinol, isopropylmethylphenol, azulene, salicylic acid, zinphylthione, benzalkonium chloride, Photosensitizer No. 301, mononitrourecosodium sodium, undecylenic acid, etc. are mentioned.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium fmarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, and the like.

Higher alcohols, such as cetyl alcohol, are mentioned as said alcohol.

In addition, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5% weight percentage with respect to a total weight, More preferably 0.01-3% by weight.

The cosmetics prepared by containing the extract of Chamonahizomi of the present invention may take the form of a solution, an emulsion, a viscous mixture, and the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in pharmaceutical compositions and cosmetic compositions in addition to the compound as an active ingredient, for example, stabilizers, solubilizers, vitamins, pigments and flavors and Such conventional adjuvants and carriers.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example 1 Preparation of Chomyeonchochomi Crude Extract

Example 1-1. Preparation of Methanol Hichomi Root 95% Ethanol Soluble Crude Extract (POC-1)

1 kg of dried Chonnahichomi root was added to 10 L of 95% (mass%) ethanol, refluxed with an extractor for 3 hours, and then slowly cooled to room temperature. After cooling, the resultant was filtered using filter paper (Whatman No. 5, Whatman International Ltd, UK), and the filtrate was concentrated using a rotary concentrator to obtain 95.6 g of extract (POC-1).

Example 1-2. To 1-11.

To extract the solvent in the same manner as in Example 1-1 using the solvent of Table 1 and the results are shown in Table 1 below.

TABLE 1

Example 1-12. Preparation of Chonnahichomi Eggplant 95% Ethanol Soluble Crude Extract (POC-12)

1 kg of dried true Nachochomi eggplant was added to 10 L of 95% (mass%) ethanol, refluxed with an extractor for 3 hours, and then slowly cooled to room temperature. After cooling, the resultant was filtered using filter paper (Whatman No. 5, Whatman International Ltd, UK), and the filtrate was concentrated using a rotary concentrator to obtain 76.4 g of extract (POC-12).

Example 1-13. To 1-17.

Using the solvent described in Table 2 below and extracted in the same manner as in Example 1-12 and the results are shown in Table 2 below.

TABLE 2

Example  2. Really hitchomi  Preparation of Nonpolar Solvent Soluble Extract

2-1. Really hitchomi  n- Hexane  Soluble extract ( POC -18)

30 g of the extract (POC-6) solid obtained in Example 1-6 was dispersed in 500 ml purified water, and 500 ml of n-hexane was added thereto, shaken for 2 to 3 hours at room temperature, and then divided into an upper layer and a lower layer. The supernatant (n-hexane) is recovered. The extract obtained by performing the same method three times was concentrated under reduced pressure to obtain 4.8 g of an extract fraction (POC-18) in powder form.

2-2. Really hitchomi  Soluble ethyl acetate extract ( POC -19)

500 ml of ethyl acetate was added to the lower layer solution (water layer) obtained in Example 2-1, shaken and left for about 2 to 3 hours at room temperature. The mixture was partitioned into an upper layer and a lower layer to recover the supernatant solution (ethyl acetate). The extract obtained by performing the same method three times was concentrated under reduced pressure to obtain 6.3 g of an extract fraction (POC-19) in powder form.

Example  3. Really hitchomi  Polar solvent soluble Radish  Produce

3-1. Really hitchomi  n- Butanol  Soluble extract ( POC -20)

500 ml of n-butanol was added to the lower layer liquid (water layer) obtained in Example 2-2, and the mixture was left to stand at room temperature for 2 to 3 hours, and then divided into an upper layer and a lower layer to recover the supernatant liquid (butanol). The extract obtained by performing the same method three times was concentrated under reduced pressure to obtain 12.8 g of an extract fraction (POC-20) in powder form.

3-2. Really hitchomi  Water soluble extract ( POC -21)

The lower layer solution (water layer) obtained in Example 3-1 was concentrated under reduced pressure to obtain 11.3 g of an extract fraction (POC-21) in powder form.

Experimental Example  One. Really hitchomi  Cytotoxicity of Extracts

Human skin fibroblasts were cultured to measure the cytotoxicity of the extract of Mt. Nachomichomi, and MTT assay method described in the literature (AA Van de Loosdrecht, E. Nennie, GJ Ossenkoppele, and RH Beelen, Langenhuijsen MM. J. Immunol.Method, 141 (1), 15, 1991).

MTT assay is an assay used to measure cell proliferation and toxicity. It is yellow water-soluble salt MTT (3- (4,5-dimethylthiazol-2-yl) by succinate dehydrogenase or mitochondrial dehydrogenase in mitochondria of living cells. The formazan derivative produced by the method of reducing -2,5-diphenyltetrazolium bromide) to a water-insoluble purple formazan derivative was measured by dissolving a solubilizer (DMSO or 2-propanol) and measuring the absorbance.

Human fibroblasts (CRL-2076, ATCC, USA) were seeded in 24-well culture plates at a concentration of 1 × 10 5 cells / well. Medium was used IMDM (Iscove's Modified Dulbecco's Medium; GIBCO, USA) containing 10% fetal bovine serum. After 24 hours of inoculation, the cells were replaced with IMDM without serum and incubated in a 37%, 5% CO ₂ incubator for 1 day after the addition of the extracts. MTT solution (2.5 mg / mL) was added 0.1 mL per well to remove the MTT solution after 4 hours and DMSO was added 1 mL per well. After dissolving the formazan derivative at room temperature, the absorbance was measured at 570 nm, and the difference between the negative control and the absorbance was shown in Table 3.

[Table 3]

As shown in the results of Table 3, it was confirmed that Mt. nachochomi extract is not toxic to fibroblasts.

Experimental Example  2. Free radical Scatters

Using the stable DPPH (1,1-Diphenyl-2-picrylhydrazyl) by improving the method of Fujita and the like, the free radical scavenging effect of the extracts and fractions of Examples 1 to 21 It was measured as follows (MS Blois, Antioxidant determinations by the use of a stable free radical, Nature, 29, pp 1199-1200, 1958).

Add 2 mL of Cham-nachochomi extract and fractions fractionated from it to 1 mL of 0.2 mM DPPH solution (SIGMA, D9132) for each concentration, react for 10 minutes at room temperature, and measure the absorbance at 525 nm. Find (%). In this case, Blanc uses ethanol instead of DPPH, and the control group adds 2 mL of ascorbic acid solution at the same concentration as the experimental group.

Free radical scavenging activity SC50 was expressed as the concentration of the extract and the compound required for 50% scavenging of free radicals (µg / ml), and the results of the extracts of Ms. nachomichomi are shown in Table 1 below, Ms. nachomichomi Results for fractions fractionated from are shown in Table 4 below.

[Table 4]

As a result of the experiment, as shown in Table 4, the extract and fractions of the present invention, Nachochochomi extract showed lower antioxidant activity than the strong antioxidant used as control, but showed similar values or antioxidant properties. It showed a high free radical scavenging ability was confirmed an excellent antioxidant effect.

Experimental Example  3. Elastase  Inhibitory effect measurement

In order to determine the elastase inhibitory effect of the Cinnamyochomi extract was tested as follows (James A.E.K., Timothy D.W. and Gordon L. Biochemistry., 35, 9090-9096, 1996). The 0.267 M Tris solution was prepared at pH 8.0, the elastase substrate was prepared with the Succ-Ala-Ala-Ala-p-nitroanilide standard at a concentration of 8.8 mM, and the porcine pancreatic elastase. An enzyme solution was prepared at a concentration of 10 μg / mL of the standard product. Absorbance A was measured using 100 μL of purified water instead of the sample in the same manner as the test solution, and the absorbance A was measured using 120 μL of purified water instead of the enzyme solution and the sample solution. The absorbance D was measured.

To 60 μL of the buffer solution prepared above, 20 μL of the substrate solution and 100 μL of the sample of Chonnahichomi extract were mixed, 20 μL of the enzyme solution was added and reacted at 25 ° C. for 15 minutes, and the amount of p-nitroaniline was produced at 410 nm. Absorbance B was measured. 20 μL of the substrate solution, 20 μL of purified water, and 100 μL of the sample were added to 60 μL of the buffer solution, and the absorbance C was measured using the solution obtained in the same manner as the control solution.

[Equation 1]

% Elastase inhibition = [1-(enzyme activity of control plant extract at each concentration / enzyme activity of control)] X 100

Elastase Inhibitory Activity IC50 was expressed as the concentration of extract and compound required to inhibit the activity of elastase by 50% (µg / mL), and the rate of elastase inhibition was calculated by Equation 1 below. Table 5 shows.

TABLE 5

As a result of the experiment, as shown in Table 5, the extract of Cham, Nachochomi of the present invention showed a similar or superior elastase inhibitory effect compared to the control group, and was effective in improving wrinkles.

Experimental Example  4. Really hitchomi  Extract and In fractions  by MMP -One mRNA  Expression inhibition effect measurement

MMP-1 (Matrix Metallo- Proteinase-1), which promotes collagen breakdown in the skin, is stimulated by UVA and subjected to reverse transcription polymerase chain reaction (RT-PCR) to see the inhibitory effect of MMP-1 gene expression. . Human fibroblasts were inoculated in a 60 mm cell culture dish at a density of 1 × 10 ⁶ , and then cultured in a 37 ° C., 5% CO ₂ incubator for 24 hours. After irradiating with UVA 6J, the extract of Chamdohichomi extract was added for each concentration and fraction and incubated for 24 hours, and 1 mL of trizol (Trizol, invitrogen, USA) was added to the cells and separated according to RNA separation method of Invitrogen. . RNA was quantified at 260 nm using an ultraviolet detector, followed by RT-PCR. RT-PCR was used RT-PCR Kit (All-in-one RT-PCR Kit, SuperBio, Korea), primers and reaction conditions are shown in Tables 6 and 7 below, the experiment was performed by the kit method .

TABLE 6 MMP-1 Primer

TABLE 7 Actin primer

The results of measuring the inhibitory effect of MMP-1 expression were expressed by correcting the actin gene, and EGCG (Epigallocatechin-3-gallate) was used as a control for the activity comparison. The results are shown in Table 8 below.

[Table 8]

Manufacturing example  1. flexible lotion ( skin Manufacturing

Table 9 shows an example of the preparation of the flexible lotion (skin) containing Mt.

TABLE 9

Manufacturing example  2. Preparation of nutrient lotion (lotion)

Formulation examples of the lotion containing Chinnamyochomi extract are shown in Table 10 below.

TABLE 10

Manufacturing example  3. Preparation of nutrition cream

An example of the preparation of the nourishing cream containing the extract of Chonnahichomi is shown in Table 11 below.

TABLE 11

Manufacturing example  4. Preparation of Hydrophilic Ointment

TABLE 12

clinical Test Example  1. Improve skin elasticity

In order to examine the effect of Cham-nachochomi extract on skin wrinkles, the lotion of Formulation Example 2 and Comparative Formulation Example 2 of Preparation Example 2 was used as a female layer of 20-55 years old, and the owner of normal, oily, dry, and complex skin 20 1 week, 2 weeks, 3 weeks, and 4 weeks after application of the lotion of Example 2 on the left side of the face and the lotion of Comparative Example 2 on the right side of the face. To measure the elasticity of the skin using a skin elasticity measuring device (Ballistometer) is shown in Table 13 below.

TABLE 13

As a result, as shown in Table 13, it was found that the lotion of Example 2 containing the true extract of the present invention, the skin elasticity effect is very excellent by the skin condition improvement effect compared to the lotion of Comparative Example 2 Could.

Clinical Experiment  2. Experiment to confirm skin condition improvement effect

In order to confirm the effect of improving skin condition on the nutritional cream of Example 3 and Comparative Formula 3, the following clinical confirmation experiment was carried out. Fifty-five owners of normal, oily, dry, and combination skins were composed of 25% of women aged 20 to 55 years old to investigate the improvement of skin condition and the skin's oil and moisture status. To the same person, the nutrition cream of Formulation Example 3 was applied to the upper part of the face, and the nutrition cream of Comparative Example 3 was applied to the skin once a day for 20 days once a day after washing the face every morning. The degree was measured. The test results are shown in Table 14.

[Table 14]

As a result of the investigation, it was found that the skin improvement effect was superior to that of Comparative Example 3, which was prepared without the addition of the cosmetic extract of Formulation Example 3, which was prepared by adding the extract.

As described above, according to the present invention, Cham, Nachochomi extract has antioxidant, anti-elastase inhibitory effect, collagenase (MMP-1) expression inhibition and tyrosinase inhibitory effect such as antioxidant, anti-wrinkle effect, whitening It can be seen that it is a natural material with excellent efficacy and no stability problems. Therefore, the pharmaceutical composition and the cosmetic composition using the true Nachochochomi extract of the present invention may be used in various fields.

Claims (8)

A skin external pharmaceutical composition for improving wrinkles, comprising Chonnahichomi (Polystichum ovatopaleaceum var. Coraiense (H.Christ) Sa.Kurata) extract as an active ingredient. According to claim 1, wherein the Cinnamyochomi extract external cosmetic composition for skin, characterized in that contained in 0.01 to 50% by weight based on the total weight of the composition. The method of claim 1, wherein the cinnamyochomi extract is an external composition for skin, characterized in that to suppress the elastase (Elastase) activity to improve the wrinkles. The method of claim 1, wherein the extract is true Nachochochomi extract, characterized in that the crude extract extracted with a solvent selected from the group consisting of water, lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol and glycerin or a mixed solvent thereof Skin external pharmaceutical composition. According to claim 4, wherein the pharmaceutical composition is an external skin pharmaceutical composition, characterized in that the solid extract of the crude extract containing a soluble extract extracted with any one solvent selected from the group consisting of hexane, ethyl acetate, butanol, and water Composition. The method of any one of claims 1 to 5, wherein the external skin pharmaceutical composition is selected from creams, gels, patches, sprays, ointments, warnings, lotions, linings, pastas and cataplasmas. Skin composition for external use, characterized in that the formulation. A cosmetic composition for external skin improvement for wrinkles, comprising Chondohichomi (Polystichum ovatopaleaceum var. Coraiense (H.Christ) Sa.Kurata) extract as an active ingredient. According to claim 7, wherein the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence Cosmetic composition, characterized in that the formulation of any one of a pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.