JP2003321377A - Antioxidant and skin care preparation obtained using the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an antioxidant exhibiting good preservation stability and an excellent antioxidant effect, and a skin care preparation. <P>SOLUTION: The antioxidant comprises as the effective ingredient extracts of one or more plants selected from the group of plants belonging to the families of Blechnaceae and Dryopteridaceae of the order of Filicales. The skin care preparation comprises the antioxidant. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to the family Blechnaceae (hereinafter, Latin notation indicating the family is omitted), the family Fern (Dryopterid).
aceae, the Latin notation for the family is omitted below)
Anti-aging agent containing an extract obtained from a plant belonging to as an active ingredient, a cell activating agent and a skin external preparation containing the same, in particular, a skin having an excellent anti-aging effect on the skin for improving wrinkles, sagging, etc. Regarding external preparations.

[0002]

2. Description of the Related Art Conventionally, for external skin preparations such as emulsions, creams, lotions, packs, detergents, dispersions, ointments, solutions, aerosols, patches, poultices, liniments, etc., they have a predetermined medicinal effect. Various medicinal components are added for the purpose of giving. For example, in order to prevent or ameliorate skin aging such as wrinkles and sagging of the skin caused by aging and exposure to ultraviolet rays, tension and deterioration of elasticity, cell activation of vitamin A, soybean extract, seaweed extract, etc. Agents have been added.

[0003]

However, these cell activators do not have sufficient anti-aging effect,
Alternatively, the desired drug effect may not be obtained due to deterioration in the formulation, and improvement thereof has been desired. The object of the present invention is to have a good anti-aging effect, good storage stability, and an anti-aging agent that can be stably compounded in a skin external preparation etc., with a cell activator and a good anti-aging effect, It is intended to provide an external preparation for skin having good storage stability.

[0004]

[Means for Solving the Problems] The present inventors have conducted diligent studies on components having an excellent antiaging effect and which can be used as a medicinal component of an external preparation for the skin. It was also found that an extract obtained from a fern plant belonging to the family Ferns has a high antiaging effect and is an excellent antiaging agent. And, it was found that this extract can be added to a skin external preparation as an anti-aging agent, and by combining with other medicinal components, a superior effect as a skin external preparation for anti-aging can be obtained. Was completed.

That is, in order to solve the above-mentioned problems, the present invention provides an anti-aging agent containing, as an active ingredient, an extract of one or more plants selected from the group of plants belonging to the family Fernidae and Fernidae. I will provide a.

[0006] As one embodiment of the present invention, the above-mentioned anti-aging agent which is a cell activator; wherein the plant is selected from the group of plants belonging to the genus Blechnum and the genus Polystichum. Anti-aging agent: a plant belonging to the genus Hleusidae (Blechnum) of the Shishigashira family is Blechnum fluviati
le) and Brechnum Discolor (Blechnum)
The above-mentioned anti-aging agent which is a plant selected from among the above;
The above-mentioned anti-aging agent, wherein the plant belonging to m) is Polystichum vestitum;

From another point of view, according to the present invention, an external preparation for skin containing the above-mentioned anti-aging agent; and a kind of a medicinal agent selected from other cell activating agents, antioxidants, humectants and ultraviolet ray inhibitors. Alternatively, the external preparation for skin containing two or more kinds is provided.

In the present specification, "antiaging agent"
The term "agent" means an agent having an anti-aging effect on living cell tissues. In particular, the "anti-aging agent" for the skin prevents wrinkles and sagging of the skin (including the scalp, lips, and mucous membranes) caused by aging, exposure to ultraviolet rays, etc. It means an agent showing an antiaging effect such as improvement. As long as these effects are exhibited, the mechanism for expressing the antiaging effect is not particularly limited. In the present specification, for example, a cell activating agent that exhibits an antiaging action by "cell activation" is also included in the range of "antiaging agent".

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION The anti-aging agent of the present invention contains, as an active ingredient, an extract of one or more plants selected from the group of plants belonging to the family Fernidae and Fernidae of the order Fern. The fernidae used in the present invention includes the genus Blechnum and the like, and examples thereof include, for example, Brecnum capense (B.
lechnum capnse, Blechnum discolor, Blechnum procerum
um), Bleknam Fluvia tile (Blechn
um fluviatile, Blechnum montanum, Blechnum durum, Blechnum amabil
e), Brechnum b
uchtienii), Blechnum castaneum, Blechnum colensoy (Blechnum colenso)
i), Breknam Psychadifolium (Blech
num cycadifolium), Blechnum hancocki
i), Brechnum
hastatum), Blechnum indicium (Blechnumindicum), Blechnum nigram (Blechnum nigram), Blechnum niponicum (Blechnum niponic)
um), Bleknum Oriente (Blechnu)
(oriental) and the like.

[0012] Further, the members of the fern order Ferns used in the present invention include Polystichchus.
m), etc., which belong to, for example, Polystice Vestimum (Polystic).
hum vestitum), polysticum poly brep balam (Polysichum poly)
blepbarum), Polystichum fibryrosopareaseumu (Polysichum fibr)
illosopalaceum), Polysiccum longiflorum (Polysichum lon)
gifrons), Polysticum Ober (P
Polysichum obae), Polysticum Ronchitis (Polysichum lonc)
hitis), Polysticium excimium (P
polystichum eximium), Polysticum Atkinsonii (Polysichum)
atkinsonii), Polysticum Piceopiumaceop
aleaceum) and the like. These fern plants were once used as folk medicines such as food, stomatitis medicine, stomach medicine and wound medicine.

In the fern plant extract used in the present invention, the extraction site is not particularly limited, and may be an extract of any part of the fern plant such as root, stem, stem, leaf and spore. Further, the skin external preparation of the present invention may be used as a mixture of extracts obtained from two or more parts of the fern plant, or an extract extracted from two or more parts with different solvents. You may mix and use 2 or more types.

The fern plant extract can be obtained by extracting one or more sites such as roots, stems, stems, leaves or spores with a suitable solvent. These portions may be subjected to a treatment such as drying, shredding, squeezing or fermentation, and then subjected to an extraction treatment. The extraction can be carried out by immersing the fern plant in a solvent for a predetermined time at low temperature or while heating. The extraction solvent is not particularly limited, and examples thereof include water; lower monohydric alcohols such as methyl alcohol and ethyl alcohol; liquid polyhydric alcohols such as glycerin, propylene glycol and 1,3-butylene glycol; ketones such as acetone; ethyl. One or more ethers such as ether; esters such as ethyl acetate; and the like can be used.

The fern plant extract may be blended as it is in an external preparation for skin, or may be used after being left as it is for a suitable period of time for aging. If necessary, purification treatment such as deodorization and decolorization can be performed by using filtration or an ion exchange resin, etc. within a range that does not affect the effects of the present invention. Further, a fraction having high activity can be taken out and used by using a separation means such as liquid chromatography.

As an example of a preferable method for extracting the fern plant, hydrous ethyl alcohol or 1,3-butylene glycol having a concentration of 0 to 100 vol% is used, and extraction is performed at room temperature or with heating for 1 to 5 days. After that, filtration is performed, and the obtained filtrate is left to stand for another week for aging to be aged, followed by filtration again. However, the extraction method is not limited to this.

The anti-aging agent of the present invention is not particularly limited in its form as long as it contains a fern plant extract as an active ingredient. It can be used in any form such as liquid form, paste form and gel form. Alternatively, a liquid extract or the like can be dried to be a solid form, or can be dried by spray drying or the like to be used as a powder.

The antiaging agent of the present invention exhibits an excellent antiaging effect exhibited by the fern plant extract as an active ingredient. The anti-aging agent of the present invention, in particular, prevents or ameliorate aging of the skin such as wrinkles and sagging of the skin caused by exposure to ultraviolet rays and the like, deterioration of tension and elasticity,
It has an excellent anti-aging effect on the skin. This anti-aging effect is presumed to be brought about by the cell stimulating effect and the like exhibited by the fern plant extract.

The content of the fern plant extract (dry solid) in the external preparation for skin of the present invention is preferably 0.00.
001 to 5 mass% (hereinafter simply indicated as "%"), and more preferably 0.001 to 0.5%. Within this range, the fern plant extract can be stably blended, and a high anti-aging effect (cell activation effect) can be exhibited. When the extract is used as the solution, the concentration of the extract is not limited as long as the content of the dry solid matter as the solute is within the above range.

The external preparation for skin of the present invention can be prepared by blending the fern plant extract as an anti-aging agent and a cell activating agent into various forms of bases according to a conventional method to prepare a preparation. Furthermore, by blending the fern plant extract in combination with one or more other medicinal agents, it is possible to prepare an external preparation for skin with a further enhanced antiaging effect.

As a preferred embodiment of the present invention, (A)
With one or more of the fern plant extract as a component,
Examples of the component (B) include a skin external preparation containing one or more kinds of medicinal agents selected from other cell activating agents, antioxidants, humectants, and UV inhibitors. As an embodiment of the present invention, the medicinal agent used as the component (B) is selected from other cell activating agents, antioxidants, moisturizers and UV inhibitors, and specific medicinal agents include Examples are shown below. In the following specific examples, the “derivative” includes an ester and a salt that can be formed. Further, those having two or more kinds of drug efficacy are shown as specific examples of each drug efficacy in duplicate.

(Cell activating agent) Cell activating agents include carotenoids (carotene, lycopene, astaxanthin, etc.), vitamin A and its derivatives (retinol palmitate, retinol acetate, etc., and their derivatives;
Retinal such as dehydroretinal and its derivatives), vitamin C and its derivatives (dipalmitic acid-L)
-L-ascorbic acid alkyl ester such as ascorbyl or tetraisopalmitic acid-L-ascorbyl, L-
Ascorbic acid phosphoric acid ester, L-ascorbic acid sulfuric acid ester, etc.), vitamin B and its derivatives (thiamine hydrochloride, thiamine sulfate, riboflavin, riboflavin acetate, pyridoxine hydrochloride, pyridoxine dioctanoate, flavin adenine dinucleotide, cyanocobalamin, Folic acid, nicotinic acid such as nicotinic acid amide and benzyl nicotinate, choline, etc.), ribonucleic acid and salts thereof,
Deoxyribonucleic acid and salts thereof, α- and γ-linolenic acid, xanthine and derivatives thereof (caffeine etc.), amino acids and derivatives thereof (serine, glutamic acid, theanine, hydroxyproline, pyrrolidonecarboxylic acid etc.),
Docosahexaenoic acid and its derivatives, eicosapentaenoic acid and its derivatives, citric acid, lactic acid, malic acid, succinic acid, almond extract, asparagus extract, apricot extract, ginkgo extract, yellowfin extract , Barley extract, kiwi extract, cucumber extract, shiitake extract, horsetail extract,
Senburi extract, soybean extract, jujube extract, Centella asiatica extract, capsicum extract, quince extract, tomato extract, garlic extract, carrot extract, hinokitiol, bukuro citrus extract, grape seed oil, Beech tree extract, beech bud extract, peach extract, eucalyptus extract, lily extract, lettuce extract, lemon extract, rosemary (mannen wax) extract, malt root extract, animal-derived extract (squid etc.) Mollusc extract, shell extract, shellfish extract, fish meat extract, poultry extract, royal jelly, silk protein and its degradation products, placenta extract, serum deproteinization extract, lactoferrin or its degradation products, etc.), yeast Examples include extracts, microbial fermentation metabolites (derived from lactic acid bacteria, bifidobacteria, etc.), and extracts of Ganoderma lucidum. In addition,
In specific examples of the extract, the names in parentheses are plant aliases, crude drug names, and the like.

Of these cell activating agents, particularly preferred are vitamin A and its derivatives and vitamin C.
And its derivatives, vitamin B and its derivatives, citric acid, malic acid, pyrrolidonecarboxylic acid, carrot extract, serum deproteinization extract, yeast extract, microbial fermentation metabolites (derived from lactic acid bacteria, bifidobacteria, etc.), Reishi extract Things can be mentioned.

(Antioxidant) In addition, as an antioxidant, vitamin E and its derivative (dl-α (β, γ) -tocopherol, dl-α-tocopherol acetate, nicotinic acid d)
Tocopherols such as 1-α-tocopherol, dl-α-tocopherol linoleate, dl-α-tocopherol succinate and its derivatives, ubiquinones, etc., vitamin A and its derivatives (retinol palmitate, retinol acetate, and the like). Derivatives, retinals such as dehydroretinal and their derivatives), carotenoids (carotene, lycopene, astaxanthin, etc.), quercetin, dibutylhydroxytoluene, butylhydroxyanisole, vitamin B and its derivatives (thiamine hydrochloride, thiamine sulfate, riboflavin, acetic acid) Riboflavin, pyridoxine hydrochloride, pyridoxine dioctanoate, flavin adenine dinucleotide, cyanocobalamin, folic acid, nicotinamide, nicotinic acid such as benzyl nicotinate , Etc.), vitamin C and derivatives thereof (dipalmitic acid-L-ascorbyl and tetraisopalmitic acid-L-ascorbyl etc., L-ascorbic acid alkyl esters, L-ascorbic acid phosphoric acid esters, L-ascorbic acid sulfuric acid esters Etc.), vitamin D
And its derivatives (ergocalciferol, cholecalciferol, dihydroxystanal, etc.), rutin, thiotaurine, taurine, hydroquinone and its derivatives, histidine, catechin and its derivatives, glabridine, glabren, liquiritin, isoliquiritin and licorice extract containing these. Substance, glutathione and its derivatives, gallic acid and its derivatives, cholesterol and its derivatives, superoxide dismutase, mannitol, cucumber extract, caycet extract, gentian (gentian) extract, gem ginger extract,
Hawthorn extract, peony extract, ginkgo extract,
Scutellaria baicalensis (carrot) extract, carrot extract, Maikai (Makai, Hermanus) extract, Sunpens (Kawara-Ketsumei) extract, Tormentilla extract, parsley extract, grape extract, Botan (Panpi) extract, Mokka (Bokeh) Extract, Melissa extract, Yashajitsu (Yasha) extract, Yukinoshita extract, Rosemary (mannen wax) extract, Lettuce extract, Tea extract (Oolong tea, black tea, green tea, etc.), Microbial fermentation metabolite, Seaweed extract , Ganoderma lucidum extract, eggshell membrane extract, placenta extract, Rakan fruit extract and the like. In the specific examples of the extract, the names in parentheses are plant aliases, crude drug names, and the like.

Among these antioxidants, particularly preferred are vitamin E and its derivatives, vitamin C and its derivatives, rutin, yashajitsu extract, yukinoshita extract, mite squid extract, superoxide dismutase, ginkgo extract. , Glutathione and its derivatives, histidine, mannitol, and carotenoids.

(Humectant) As the moisturizer, glycerin,
1,3-butylene glycol, proteins, derivatives thereof and hydrolysates thereof and salts thereof (collagen, elastin, keratin etc.), mucopolysaccharides and derivatives thereof (hyaluronic acid, chondroitin sulfate etc.), amino acids and derivatives thereof (histidine, Serine, glycine, theanine,
Aspartic acid, arginine, pyrrolidonecarboxylic acid, etc.), sugars (sorbitol, erythritol, trehalose, inositol, glucose, xylitol, sucrose and its derivatives, dextrin and its derivatives, honey, etc.), D-panthenol and its derivatives, glycolipids, Ceramide, hot spring water, armature extract, almond extract,
Ashitaba extract, avocado extract, altea extract, arnica extract, aloe extract, mung bean extract,
Scutellaria baicalensis (Ouchon) extract, Coptis chinensis extract, Hypericum perforatum extract, Odorichosou extract, Ononis extract, Chamomile extract, Oat extract, Grabridin, Grabrene, Liquiritin, Isoliquiritin and licorice extract containing these, raspberry extract , Honeysuckle extract, quince seed (quince) extract, clara (kujin) extract, gardenia extract, kumazasa extract, grapefruit extract, watercress extract, gentian (gentian) extract, genus ginger extract, Burdock extract, wheat extract, comfrey (spinach) extract, cinnamon extract, cactus extract, sabbath extract, salvia (sage) extract,
Hawthorn extract, diorhea extract, perilla extract, spirea extract, peony extract, ginger extract, ginger extract, birch extract, peppermint (peppermint, green mint, spearmint, etc.) extract, mallow (Pleurotus ostreatus) Extract, senkyu extract, mulberry extract, pacific (thyme) extract, camellia extract, Japanese tuki extract, euglena extract, dokudami extract, tormentilla extract, parsley extract, adzuki bean (yokuinin) Extract, Hamamelis (witch hazel) extract, rose extract, cypress extract,
Sunflower extract, coltsfoot extract, butchers bloom extract, grape extract, prune extract, loofah extract, bodige extract, peony extract, hop extract, pine extract, horse chestnut extract , Mukurodi extract, mucin, melissa extract, cornflower extract, yukinoshita extract, lily extract, lime extract, lavender extract, apple extract, gentian (ryutan) extract, phospholipids and their derivatives (soybean, Derived from egg yolk, etc.), Astragalus extract, Waremoko (Jiuyu) extract, Tea (Oolong tea, green tea, black tea, etc.) extract, Urea, Rakan fruit extract, Seaweed extract (kelp, mackerel, wakame, hijiki, hibamata, swallow, Brown algae such as Tororocombu, Kajime, Tsuruameme, Chigaiso, Honda straw, Giant kelp; Red seaweeds such as sa, giant ginkgo, giraffe, tsunomata, horse mackerel, usbanori, asakusanori, pine nuts, tosakamatsu, funori, gogonori, kamensou, igisu, ego nori; chlorella, aonori, donariera, chlorococcus moss, anana aosa. Green algae such as Casa Nori, Fusuzuzumo, Tamajusomo, Aedes aegypti, Aedoro and the like; cyanobacteria such as Spirulina) and the like. Furthermore, as a moisturizing (emollient) agent by sealing the skin surface, jojoba oil, macadamia nut oil, olive oil, apricot kernel oil, persic oil, safflower oil, sunflower oil, avocado oil,
Meduhome oil, camellia oil, almond oil, perilla oil,
Examples include sesame oil, borage (borage) oil, cocoa butter, and shea butter. By blending these moisturizers,
A higher antiaging effect can be exhibited. In addition,
In specific examples of the extract, the names in parentheses are plant aliases, crude drug names, and the like.

Of these moisturizers, amino acids and their derivatives, phospholipids and their derivatives, glycerin and 1,3-butylene glycol are particularly preferable.

(Ultraviolet Preventing Agent) As an ultraviolet preventing agent,
2-Ethylhexyl paramethoxycinnamate, 4-te
rt-Butyl-4′-methoxydibenzoylmethane, oxybenzone and its derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, 2-hydroxy-4
-Methoxybenzophenone-5-sulfonate, etc.), titanium oxide, particulate titanium oxide, zinc oxide, particulate zinc oxide and the like. Particularly, fine particles of the inorganic powder such as titanium oxide and zinc oxide exhibit higher effects.

Among these ultraviolet ray inhibitors, particularly preferable are 2-ethylhexyl paramethoxycinnamate, titanium oxide, fine particle titanium oxide, zinc oxide and fine particle zinc oxide.

The compounding amount of the above-mentioned component (B) component in the external preparation for skin of the present invention varies depending on the kind of the drug, but it is preferably within the following range. Within this range, when combined with the fern plant extract of the component (A), the formulation and the stability of the component (A) in the formulation over time are not affected, and a higher antiaging effect of the present invention is obtained. Can be demonstrated.

That is, the blending amount of the other cell activator in the external preparation for skin of the present invention is preferably 0.00001 to 10.
%, And more preferably 0.0001 to 5%. When the extract is used as it is, the dry solid content may be within this range. Within this range, a skin external preparation that exhibits a more excellent cell activation effect and an excellent antiaging effect on the skin such as improving wrinkles and sagging can be obtained.

The content of the antioxidant in the external preparation for skin of the present invention is preferably in the range of 0.00001 to 5%, more preferably 0.0001 to 3%. When the extract is used as it is, the dry solid content may be within this range. Within this range, an excellent anti-oxidant effect is exerted, damage to the skin dermis due to active oxygen or the like is prevented, and a skin external preparation having a superior anti-aging effect is obtained.

The amount of the moisturizing agent in the external preparation for skin of the present invention is preferably 0.00001 to 25%, more preferably 0.0001 to 20%. When the extract is used as it is, the dry solid content may be within this range. Within this range, a skin external preparation that exhibits an excellent moisturizing effect, suppresses the formation of wrinkles due to drying, etc., and exhibits a more excellent antiaging effect can be obtained.

The compounding amount of the ultraviolet protective agent in the external preparation for skin of the present invention is preferably 0.001 to 30%, more preferably 0.01 to 25%. Within this range, an excellent anti-UV effect is exhibited, and by preventing wrinkles and sagging due to ultraviolet rays, a skin external preparation exhibiting an excellent anti-aging effect can be obtained.

These other cell activators, antioxidants, moisturizers and UV inhibitors can be used alone or in combination of two or more.

Examples of the compounding form of the external preparation for skin of the present invention are not particularly limited, and include, for example, emulsions, creams, lotions, beauty essences, packs, detergents, makeup cosmetics, dispersions, ointments, liquids, It may be any form of cosmetics such as aerosols, patches, poultices, liniments and the like, or external medicines.

Further, the external preparation for skin of the present invention includes the above-mentioned (A)
In addition to the component (B), various components usually used in cosmetics, quasi drugs, external medicines, etc., that is, water, alcohols, oils, surfactants, thickeners, powders, chelating agents, p
H regulators, various medicinal agents, extracts derived from plants and animals, microorganisms,
Fragrances and the like can be appropriately added within a range that does not impair the effects of the present invention. Of these, specific examples are shown below (in parentheses after the plant name, the plant alias, crude drug name, etc. are described).

As the alcohol, monohydric alcohols or polyhydric alcohols such as ethyl alcohol can be used for the purpose of dissolution, refreshing feeling, antiseptic, moisturizing, etc., as long as they do not overlap with the essential components.

The oil agent can be used irrespective of its origin and properties in order to improve usability and usability.
For example, liquid paraffin, squalane, triglyceride oil, ester oil, waxes, fatty acids, higher alcohols,
Examples include silicone oil, fluorine-based oil, and various waxes.

The surfactant is used for emulsification and solubilization of oils and the like, and anionic, cationic, nonionic and amphoteric surfactants can be used.

As the thickener, carboxyvinyl polymer, carrageenan, agar, xanthan gum, dextrin fatty acid ester, organically modified clay mineral, etc., whether they are chemically synthesized products or natural products, may be used as long as they do not overlap with the essential components. It is possible. Further, these components can be used not only for adjusting the viscosity of the system, but also for gelling, moisturizing, film formation and the like.

The powder may be compounded or surface-treated in order to improve usability and usability regardless of shape, particle size, presence or absence of porosity, crystal structure and the like.
Inorganic powders such as talc, mica, sericite, silicic acid anhydride, organic powders such as nylon powder, fish scale foil, pearl pigments such as bismuth oxychloride, iron oxide, carbon black, inorganic pigments such as ultramarine, tar pigments and The lake, natural pigment, etc. are used depending on the application.

In order to prevent the quality deterioration of the components in the system, EDT
A chelating agent such as A and a pH adjusting agent with a buffer such as lactic acid-sodium lactate can also be used.

Antibacterial agents and bactericides include benzoic acid, sodium benzoate, for the purpose of preventing and improving acne and the like.
Paraoxybenzoic acid ester, parachlorometacresol, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol and the like are used.

The whitening agent is used for the purpose of suppressing the production of melanin, preventing and improving pigmentation such as dull skin, spots, freckles or senile pigment spots and liver spots, and improving the transparency of the skin. . Examples include sempukuka extract, agarose, oligosaccharides, cysteine and its derivatives, acerola extract, ibukitrano extract, age extract, eleuthero extract, pea extract and other endothelin antagonists, camu camu extract, orange. Examples include extracts, coffee extracts, linoleic acid and vegetable oils containing linoleic acid, gokahi extracts, rice extracts, rice bran extracts, white lily extracts, blackcurrant extracts, spinach extracts, molasses extracts and the like.

Examples of the anti-inflammatory agent include glycyrrhizic acid and its derivatives, glycyrrhetinic acid and its derivatives, sulfur and its derivatives, nettle extract, ginkgo biloba extract, turmeric extract, elder extract, cattail (mudder) extract, mugwort extract. Examples include extracts. By incorporating an anti-inflammatory agent, effects such as suppression of skin pigmentation due to inflammation and prevention of aging can be imparted.

The blood circulation promoter is used for the purpose of preventing wrinkles and a decrease in elasticity by promoting blood flow in the skin, and examples include capsicum tincture, γ-oryzanol and the like, and examples of the enzyme include lipase, papain and the like. To be By blending these, a higher antiaging effect can be exhibited.

[0046]

The present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to the following examples.

(Example 1: Production of fern plant extract) Blechnum fluviatile (Blechnum flu) of the genus Blechnum (Blechnum)
viale) and Bleknam Discolor (Bl)
echnum discolor), Polystichum vestitum (Polystichum) of the genus Polystichum
um), for each of these fern plants, to each 500 g of the mixture of leaves and stems, purified water, 50 vol.
3 L of% hydrous ethyl alcohol or ethyl alcohol was added separately, and the mixture was extracted at room temperature or with heating for 1 day. After that, the mixture is filtered, the solvent is distilled off from the obtained filtrate, and the mixture is dried to dryness.
A vol% hydrous ethyl alcohol extract and an ethyl alcohol extract were obtained.

(Example 2: Cell activation test by cell culture) Human newborn-derived fibroblasts NB1RGB were used. Two
Proper amount of culture medium in a 4-well plate, fibroblast NB1RG
B was seeded and left standing at 37 ° C. in a carbon dioxide concentration of 5 vol%. On the next day, the final concentration of the purified water extract of each fern plant obtained in Example 1, 50 vol% hydrous ethyl alcohol extract and ethyl alcohol extract was 0.
(Control), 100, 1000, 10000 μg / mL of the sample preparation solution was added and mixed. On the 4th day of culture, the medium was exchanged, and the sample preparation solution was added again. On the next day, the medium was removed, the cells were washed with a phosphate buffer and then collected, and the number of fibroblasts NB1RGB grown in each sample preparation was evaluated as a cell growth rate compared with a control.

(Evaluation Criteria) The number of cells grown by adding each sample preparation solution was compared with the number of control cells, and the cell activation effect was evaluated using the cell growth rate as an index. The number of cells is
Counting was performed using a hemocytometer. These results are shown in Table 1.
It was shown to.

(Result)

[Table 1]

As is clear from the results of Table 1, it was confirmed that the fern plant extract used as the external preparation for skin in the present invention has a high cell activating effect on human newborn-derived fibroblasts NB1RGB. Was given. Therefore, by applying the fern plant extract to the skin, a very excellent anti-aging effect can be exerted, and wrinkles and sagging of the skin caused by aging, exposure to ultraviolet rays, etc. can be effectively improved.

(Example 3: Cream) Each cream having the composition shown in Table 2 below was prepared by the following method. Components (1) to (6) and (12) are mixed and heated to 70 ° C.
The component (14) maintained at 70 ° C. was added to the mixture maintained at 1 to emulsify. In this emulsion, components (7) to (11)
After adding (13) and (13), the mixture was cooled to obtain a cream.

(Test method) 35 per test cream
~ 15 women aged 59 years old will be on the panel every morning and evening.
After washing the face once and for 12 weeks, an appropriate amount of the test cream was applied to the face. The effect of improving wrinkles by application was evaluated according to the following criteria, and the number of people who meet each evaluation criterion is shown in Table 2.

(Evaluation criteria) <Evaluation> <Contents> Effective The wrinkles on the skin have disappeared. Somewhat effective Wrinkles on the skin are less noticeable. Ineffective No change from before use.

(Composition and Results)

[Table 2]

From the results shown in Table 2, the product of the present invention containing the fern plant extract can improve the wrinkles of the skin by applying these to the skin, and the effect of producing a beautiful skin with tension. It became clear that there is.

(Example 4: Cream) Each cream having the composition shown in Table 3 was prepared by the following method. Ingredient (1) ~
(6) and (9) to (13) are mixed and heated to 70 ° C.
Component (15) maintained at 70 ° C. was added to the mixture maintained at 70 ° C. to emulsify. Components (7) to (8) and (14) were added to this emulsion and then cooled to obtain a cream. Evaluation of the wrinkle-reducing effect of each creamy that contains this fern plant extract (purified water extract of Blechnum discolor of Shishigashira family) and a cell activating agent, antioxidant, moisturizing agent or UV inhibitor Then
Table 3 shows the number of people who meet each evaluation criterion. The test method and evaluation criteria were the same as in Example 3.

(Composition and Results)

[Table 3]

As shown in the results in Table 3, Blechnum dscar (Blechnum d
The product 2 of the present invention containing a purified water extract of iscolor)
It is clear that by applying these to the skin, the cream of No. 1 can improve the wrinkles of the skin and make the skin firm and beautiful. By applying a cream containing a combination of an oxidant, a moisturizer, and an anti-UV agent to the skin, it exhibits a more excellent skin wrinkle improving effect as compared to the case of applying the cream containing each of them alone. It was revealed that the skin will be firm and beautiful.

(Example 5: Lotion) The following components (1) to (7)
A solution prepared by mixing and dissolving and a solution prepared by mixing and dissolving the following components (8) to (11) were mixed and made uniform to obtain a lotion. (Component) (%) (1) Glycerin 10.0 (2) 1,3-Butylene glycol 6.0 (3) Purified water extract of Blechnum fluviatile * 1 0.001 (4) Glycyrrhizic acid Dipotassium * 2 0.1 (5) Citric acid 0.1 (6) Sodium citrate 0.3 (7) Purified water Remaining amount (8) Polyoxyethylene hydrogenated castor oil (60 EO) 0.5 (9) ) Ethyl alcohol 8.0 (10) Preservative Suitable amount (11) Perfume Suitable amount * 1 What was produced in Example 1 * 2 Maruzen Pharmaceutical Co., Ltd.

(Example 6: Lotion) The following components (1) to (7)
A solution prepared by mixing and dissolving and a solution prepared by mixing and dissolving the following components (8) to (11) were mixed and made uniform to obtain a lotion. (Component) (%) (1) Glycerin 10.0 (2) 1,3-butylene glycol 6.0 (3) 50 vol% hydrous ethyl alcohol extract of Blechnum discolor (Blechnum discolor) * 1 0.05 (4 ) Squid extract * 2 0.5 (5) Lactic acid 0.1 (6) Sodium lactate 0.3 (7) Purified water Remaining amount (8) Polyoxyethylene hydrogenated castor oil (60 EO) 0.5 ( 9) Ethyl alcohol 8.0 (10) Preservative Suitable amount (11) Perfume Suitable amount * 1 What was produced in Example 1 * 2 Maruzen Pharmaceutical Co., Ltd.

(Example 7: Emulsion) The following components (13) to (1)
7) was heated and mixed, and the following components (1) to (12) were heated and mixed to the mixture maintained at 70 ° C., and the mixture maintained at 70 ° C. was added and mixed to uniformly emulsify. After cooling this emulsion, components (18) to (23) were added and uniformly mixed to obtain an emulsion. (Component) (%) (1) sorbitan monostearate 0.3 (2) polyoxyethylene sorbitan monooleate (20 EO) 0.1 (3) lipophilic glyceryl monostearate 0.2 (4) ) Stearic acid 0.5 (5) Cetanol 0.5 (6) Squalane 3.0 (7) Liquid paraffin 4.0 (8) Glyceryl tri-2-ethylhexanoate 2.0 (9) Methyl polysiloxane 1. 0 (10) Hydrogenated soybean phospholipid 0.1 (11) Nicotinic acid dl-α-tocopherol * 1 0.05 (12) Preservative Suitable amount (13) Carboxyvinyl polymer aqueous solution (1.0%) 10.0 ( 14) Sodium hydroxide 0.05 (15) Glycerin 5.0 (16) 1,3-Butylene glycol 7.0 (17) Purified water Remaining amount (18) Ethyl alcohol 0.0 (19) Ethyl alcohol extract of Blechnum discolor * 2 0.0001 (20) Ginkgo extract * 3 3.0 (21) Magnesium phosphate L-ascorbyl * 4 0.1 (22) ) Hemp cellulose powder 3.0 (23) Perfume proper amount * 1 Made by Eisai Co., Ltd. * 2 Manufactured in Example 1 * 3 Made by Tokiwa Plant Science Research Institute * 4 Made by Nikko Chemicals

(Example 8: Emulsion) The following components (13) to (1)
7) was heated and mixed, and the following components (1) to (12) were heated and mixed to the mixture maintained at 70 ° C., and the mixture maintained at 70 ° C. was added and mixed to uniformly emulsify. After cooling this emulsion, the components (18) to (22) were added and uniformly mixed to obtain an emulsion. (Component) (%) (1) sorbitan monostearate 0.3 (2) polyoxyethylene sorbitan monooleate (20 EO) 0.1 (3) lipophilic glyceryl monostearate 0.2 (4) ) Stearic acid 0.5 (5) Cetanol 0.5 (6) Squalane 3.0 (7) Liquid paraffin 4.0 (8) Glyceryl tri-2-ethylhexanoate 2.0 (9) Methyl polysiloxane 1. 0 (10) Hydrogenated soybean phospholipid 0.1 (11) Rutin * 1 0.2 (12) Preservative proper amount (13) Aqueous carboxyvinyl polymer solution (1.0%) 10.0 (14) Sodium hydroxide 0 .05 (15) Glycerin 5.0 (16) 1,3-Butylene glycol 7.0 (17) Purified water Remaining amount (18) Ethyl alcohol 5.0 (19) Brennamdi 50 vol% hydrous ethyl alcohol extract of color (Blechnum discolor) * 2 0.1 (20) Sophoraceae extract * 3 1.0 (21) Silicic anhydride 3.0 (22) Perfume suitable amount * 1 Sigma * 2 Manufactured in Example 1 * 3 Maruzen Pharmaceutical Co., Ltd.

All of Examples 5 to 8 have excellent stability over time,
When applied to the skin, it was a lotion and an emulsion having an excellent anti-aging effect, which improved wrinkles and sagging of the skin due to sunburn, aging, etc., and made the skin firm and beautiful.

(Example 9: Ointment) A part of the following components (5), (6) and (7) was heated and mixed, and the mixture maintained at 75 ° C. was heated and mixed with the following components (1) to (4). And slowly added to the mixture maintained at 75 ° C. While cooling this mixture, (8) to (10) dissolved in the balance of the component (7) were added to obtain an ointment. (Component) (%) (1) Stearic acid 18.0 (2) Cetanol 4.0 (3) dl-α-tocopherol * 1 0.2 (4) Preservative A suitable amount (5) Triethanolamine 2.0 ( 6) Glycerin 5.0 (7) Purified water Remaining amount (8) Ethyl alcohol extract of Blechnum discolor * 2 0.05 (9) Dipotassium glycyrrhizinate * 3 0.5 (10) Yeast extract 0.5 * 1 Manufactured by Eisai * 2 Manufactured in Reference Example 1 * 3 Manufactured by Maruzen Pharmaceutical Co., Ltd.

(Example 10: Ointment) The following components (4) and (5)
And a part of (6) was heated and mixed, and the mixture maintained at 75 ° C. was gradually added to the mixture of the following components (1) to (3) heated and mixed and maintained at 75 ° C. While cooling this mixture, (7) to (10) dissolved in the rest of the component (6) were added to obtain an ointment. (Component) (%) (1) Stearic acid 18.0 (2) Cetanol 4.0 (3) Preservative Appropriate amount (4) Triethanolamine 2.0 (5) Glycerin 5.0 (6) Purified water Remaining amount (7) Purified water extract of Polystichum vestitum * 1 0.001 (8) Touki extract * 2 0.3 (9) Sodium pyrrolidonecarboxylate * 3 0.3 (10) Tetraiso L-Ascorbyl valmitate * 4 0.01 * 1 Manufactured in Example 1 * 2 Nippon Powder Chemicals * 3 Ajinomoto Co. * 4 Nikko Chemicals

Examples 9 and 10 were ointments having excellent stability over time, and when applied to the skin, wrinkles and sagging of the skin were improved, and the skin became firm and beautiful, and had an excellent antiaging effect.

(Example 11: Pack) The following components (1) to
To the solution prepared by mixing (6) and heating at 70 ° C., the solution prepared by mixing and dissolving the following components (7) and (8) was added and mixed. After cooling this mixed solution, the component (9)-
(11) was uniformly dispersed to obtain a pack. (Component) (%) (1) Polyvinyl alcohol 15.0 (2) Silicic anhydride 0.5 (3) Polyethylene glycol 0.5 (4) Polyoxypropylene methyl glucoside 5.0 (5) Glycerin 5.0 ( 6) Purified water Residual amount (7) Ethyl alcohol 10.0 (8) Preservative Appropriate amount (9) Purified water extract of Blechnum fluviatile * 1 0.0001 (10) Blechnum discolor Ethyl alcohol extract of * 1 0.0001 (11) Perfume Suitable amount * 1 Produced in Example 1

(Example 12: Pack) The following components (1) to
To the solution prepared by mixing (7) and heating to 70 ° C., the solution prepared by mixing and dissolving the following components (8) and (9) was added and mixed. After cooling this mixture, the component (10)
(13) were uniformly dispersed to obtain a pack. (Component) (%) (1) Polyvinyl alcohol 15.0 (2) Silicic anhydride 0.5 (3) Polyethylene glycol 0.5 (4) Polyoxypropylene methyl glucoside 5.0 (5) Glycerin 5.0 ( 6) Purified water Residual amount (7) Orange flower water * 1 10.0 (8) Ethyl alcohol 20.0 (9) Preservative Suitable amount (10) Polysticum vesitum purified water extract * 2 0.01 (11) Licorice extract * 3 0.5 (12) Wheat extract * 4 0.5 (13) Appropriate amount of flavor * 1 Made by Esperis * 2 Made by Example 1 * 3 Made by Maruzen Pharmaceutical * 4 Made by Seiwa Kasei

Examples 11 and 12 were excellent in stability over time, and when applied to the skin, the packs improved wrinkles and sagging of the skin to make the skin firm and beautiful, and were excellent in the antiaging effect.

Example 13 Liquid Foundation Components (13) to (18) were added to a solution obtained by heating and mixing and dissolving the following components (1) to (7), and the mixture was uniformly mixed and maintained at 70 ° C. The components (8) to (12) below were uniformly dissolved in this mixture, and the mixture was added to the mixture maintained at 70 ° C. to uniformly emulsify the mixture. After cooling this emulsion, the component (19)-
(22) was added to obtain a liquid foundation. (Component) (%) (1) Dipentaerythritol fatty acid ester 2.0 (2) Liquid paraffin 5.0 (3) Stearic acid 2.0 (4) Cetanol 1.0 (5) Self-emulsifying monostearic acid Glyceryl 1.0 (6) 2-Ethylhexyl para-methoxycinnamate 8.0 (7) Preservative Appropriate amount (8) Glycerin 5.0 (9) Triethanolamine 1.0 (10) Carboxymethylcellulose 0.2 ( 11) Bentonite 0.5 (12) Purified water Remaining amount (13) Titanium oxide 6.0 (14) Fine particle titanium oxide 2.0 (15) Fine particle zinc oxide 5.0 (16) Mica 2.0 (17) Talc 4.0 (18) Colored pigment 4.0 (19) Polysticum vestimum purified water extract * 1 0.1 ( 0) Mannitol * 2 0.5 (21) Nicotinic acid amide * 3 0.5 (22) as prepared in Perfume qs * 1 Example 1 * 2 Sigma * 3 Sigma

(Example 14: Liquid foundation) Components (15) to (22) were added to a solution in which the following components (1) to (9) and (24) were mixed and dissolved, and the mixture was mixed evenly, and
Maintained at 0 ° C. This mixture is mixed with the following components (10)-
(14) and (25) were uniformly dissolved, added to the mixture maintained at 70 ° C, and uniformly emulsified. After cooling this emulsion, components (23) and (26) were added to obtain a liquid foundation. (Component) (%) (1) Dipentaerythritol fatty acid ester 2.0 (2) Liquid paraffin 5.0 (3) Stearic acid 2.0 (4) Cetanol 1.0 (5) Self-emulsifying monostearic acid Glyceryl 1.0 (6) -2-Ethylhexyl paramethoxycinnamate 8.0 (7) 4-tert-Butyl-4′-methoxydibenzoylmethane 2.0 (8) Retinol palmitate * 1 0.2 ( 9) Preservative A suitable amount (10) Glycerin 5.0 (11) Triethanolamine 1.0 (12) Carboxymethyl cellulose 0.2 (13) Bentonite 0.5 (14) Purified water Remaining amount (15) Titanium oxide 6. 0 (16) Fine particle titanium oxide 2.0 (17) Fine particle zinc oxide 5.0 (18) Mica 2.0 (19) Talc 4.0 (20) Yellow iron oxide 1.0 ( 1) black iron oxide 0.05 (22) red iron oxide 0.5 (23) purified water extract of Blechnum fluviatile * 2 0.01 (24) dl-α-tocopherol acetate * 3 0.5 ( 25) Magnesium phosphate-L-ascorbyl * 4 0.5 (26) Perfume proper amount * 1 manufactured by Nippon Roche * 2 manufactured by Example 1 * 3 manufactured by Eisai * 4 manufactured by Nikko Chemicals

(Example 15: Oily foundation) Components (8) to (12) were added to a solution obtained by heating and dissolving the following components (1) to (7) and (13) to (17) at 80 ° C to homogenize them. The mixture was mixed and solidified by cooling to obtain an oily foundation. (Component) (%) (1) Candelilla wax 4.0 (2) Paraffin wax 5.0 (3) Vaseline 5.0 (4) Methyl polysiloxane 15.0 (5) Squalane 25.0 (6) Tri Diglyceryl isostearate Residual amount (7) Organically modified bentonite 3.0 (8) Glycerin 0.5 (9) Titanium oxide 10.0 (10) Sericite 5.0 (11) Nylon powder 5.0 (12) Coloring Pigment Appropriate amount (13) Alkyl-modified carboxyvinyl polymer 0.5 (14) Ethyl alcohol extract of Blechnum discolor * 1 0.02 (15) Acetic acid dl-α-tocopherol * 2 0.1 (16) Stearyl glycyrrhetinate * 3 0.1 (17) Perfume Suitable amount * 1 What was produced in Example 1 * 2 Eisai Ltd. * 3 product of Maruzen Pharmaceuticals Co., Ltd.

(Example 16: Sunscreen Emulsion) To a mixture prepared by mixing and dispersing the following components (1) to (12), a solution prepared by mixing and dissolving the following components (13) to (16) was added and uniformly emulsified. . In this emulsion, components (17) and (1
8) was added to obtain a sunscreen emulsion. (Component) (%) (1) Polyoxyalkylene-modified organopolysiloxane 1.0 (2) Dimethylpolysiloxane 5.0 (3) Octamethylcyclotetrasiloxane 20.0 (4) Isotridecyl isononanoate 5.0 (5 ) 2-Ethylhexyl paramethoxycinnamate 5.0 (6) Preservative Amount (7) Perfume Amount (8) Fine particle titanium oxide 10.0 (9) Fine particle zinc oxide 10.0 (10) Zirconium oxide 5.0 (11) Polystyrene powder 3.0 (12) Trimethylsiloxysilicic acid 0.5 (13) Dipropylene glycol 3.0 (14) Ethyl alcohol 10.0 (15) Purified water Residual amount (16) Salt 0.2 ( 17) Purified water extract of Blechnum discolor * 1 0.001 (18) Seaweed extraction * 2 3.0 * 1 Example 1 thing was prepared in * 2 Phillip lock Ray Co., Ltd.

(Example 17: Sunscreen Emulsion) To a mixture prepared by mixing and dispersing the following components (1) to (12), a solution prepared by mixing and dissolving the following components (13) to (16) was added and uniformly emulsified. . Components (17) to (19) were added to this emulsion to obtain a sunscreen emulsion. (Component) (%) (1) Polyoxyalkylene-modified organopolysiloxane 1.0 (2) Dimethylpolysiloxane 5.0 (3) Octamethylcyclotetrasiloxane 20.0 (4) Isotridecyl isononanoate 5.0 (5 ) 2-Ethylhexyl para-methoxycinnamate 10.0 (6) Preservative Suitable amount (7) Perfume Suitable amount (8) Fine particle titanium oxide 8.0 (9) Fine particle zinc oxide 7.0 (10) Zirconium oxide 5.0 (11) Polystyrene powder 3.0 (12) Trimethylsiloxysilicic acid 0.5 (13) Dipropylene glycol 3.0 (14) Ethyl alcohol 10.0 (15) Purified water Residual amount (16) Salt 0.2 ( 17) 50 vol% hydrous ethyl alcohol extract of Blechnum discolor * 10 01 (18) superoxide dismutase * 2 3.0 (19) Green tea extract * 3 0.5 * 1 Example 1 as prepared in * 2 Sigma * 3 Maruzen Pharmaceuticals Co., Ltd.

Examples 13 to 17 have excellent stability over time, and when applied to the skin, they improve wrinkles and sagging of the skin due to sunburn, aging, etc., and have excellent anti-aging effects, such as liquid foundation, oil foundation and sunscreen. It was an emulsion.

[0077]

INDUSTRIAL APPLICABILITY As described above, the present invention exhibits an excellent anti-aging effect and an excellent cell activating effect by containing a specific fern plant extract as an active ingredient, and an anti-aging agent and a cell activating agent. Is. The external preparation for skin containing the antiaging agent and the cell activating agent of the present invention exerts a high effect of improving wrinkles, and is effective for improving wrinkles and sagging of the skin due to sunburn, aging and the like. Furthermore, a specific fern plant extract and other cell activating agents, antioxidants, moisturizers, topical skin preparations of the present invention containing a medicinal component such as an anti-UV agent have a more excellent antiaging effect. Is to have. Therefore, the external preparation for skin of the present invention can be advantageously used as cosmetics and pharmaceuticals for the purpose of preventing aging.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Taku Hoshino             Kose Co., Ltd. 48-18 Sakaemachi, Kita-ku, Tokyo             -In Research Division (72) Inventor Akira Inomata             Kose Co., Ltd. 48-18 Sakaemachi, Kita-ku, Tokyo             -In Research Division (72) Inventor Masatake Yoshida             11-7 Nihonbashi Tomizawa-cho, Chuo-ku, Tokyo             Inside the medicinal industry (72) Inventor Masayo Fujimoto             11-7 Nihonbashi Tomizawa-cho, Chuo-ku, Tokyo             Inside the medicinal industry (72) Inventor Taketsugu Kodaira             11-7 Nihonbashi Tomizawa-cho, Chuo-ku, Tokyo             Inside the medicinal industry F-term (reference) 4C083 AA032 AA111 AA112 AA122                       AB032 AB172 AB212 AB232                       AB242 AB432 AB442 AC022                       AC062 AC102 AC122 AC132                       AC212 AC242 AC302 AC352                       AC422 AC432 AC442 AC542                       AC852 AD022 AD042 AD072                       AD092 AD112 AD152 AD162                       AD172 AD202 AD262 AD272                       AD392 AD532 AD572 AD622                       AD642 AD662 BB46 BB47                       BB51 CC01 CC02 CC04 CC05                       CC07 CC12 DD22 DD23 DD27                       DD31 EE06 EE12                 4C088 AA18 AC05 AC06 AC13 AC18                       AD16 BA05 BA09 BA10 CA11                       MA07 NA14 ZA89

Claims (7)

[Claims] 1. The family Blechna (Blechna)
ceae) and the family Fern (Dryopteridace)
An anti-aging agent comprising as an active ingredient one or more plant extracts selected from the group of plants belonging to ae). 2. The anti-aging agent according to claim 1, which is a cell activator. 3. The plant is the family Blechidae (Blech).
The anti-aging agent according to claim 1 or 2, which is selected from the group of plants belonging to the genus (Naceae) of the genus Blechnum and the genus Polystichum of the family Dryopteridaceae. 4. The family Blechnaceae
ae) Plants belonging to the genus Blechnum are Blechnum fulviatile (Blechnu).
The anti-aging agent according to claim 3, which is a plant selected from m fluviatile) and Blechnum discolor. 5. The Dryopteridae.
ceae) a plant belonging to the genus Polystichum (Polyacetum)
The anti-aging agent according to claim 3 or 4, which is lystichum vestitum). 6. A skin external preparation containing the anti-aging agent according to any one of claims 1 to 5. 7. The external preparation for skin according to claim 6, which further contains one or more kinds of medicinal agents selected from other cell activators, antioxidants, moisturizers and UV inhibitors.