JP2003252742A - Melanocyte dendrite formation inhibitor and skin care preparation containing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin care preparation having excellent melanocyte dendrite formation inhibitory effect, causing no deterioration in the preparation and having high bleaching effect and/or skin beautifying effect. <P>SOLUTION: The skin care preparation contains a melanocyte dendrite formation inhibitor with yeast extract as an active ingredient. Another version of the skin care preparation contains the yeast extract and at least one medicinal agent selected from a bleaching agent, antioxidant, anti-inflammatory agent, cell potentiator and ultraviolet inhibitor. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a melanocyte dendrite formation inhibitor and a skin external preparation containing the same. More specifically, the present invention relates to a melanocyte dendrite formation inhibitor characterized by using a yeast extract as an active ingredient, by containing this melanocyte dendrite formation inhibitor, dull skin, stains, The present invention relates to an external preparation for skin which is excellent in whitening effect and skin beauty effect, which prevents and improves pigmentation such as freckles, senile pigment spots, and liver spots, and improves the transparency of the skin.

[0002]

2. Description of the Related Art In response to the market demand for whitening products, which is increasing year by year, various attempts have been made to clarify the whitening mechanism and research and development of new whitening agents have been carried out. Melanin in the skin is produced by melanocytes, which are melanin-producing cells, but is produced by using tyrosine as a starting material and tyrosinase, which is a key enzyme for melanin production, as a catalyst. The reaction takes place in vesicles called melanosomes, and melanosomes mature through steps from type I to type IV. The melanosomes containing the matured melanin granules are transferred to the keratinocytes by depriving the adjacent keratinocytes of the dendrites of the melanocytes containing the melanosomes. Then, with the turnover process of keratinocytes, the collapse of melanosomes,
The melanin granules are dispersed, and finally keratinocytes containing melanin are exfoliated and excreted.
Abnormal pigmentation is a state in which melanin production is performed excessively as described above, and a large number of melanins are transferred to keratinocytes, resulting in the manifestation of pigmentation such as spots and freckles. . Conventionally, in order to prevent and improve skin blackening caused by sunburn etc., spots caused by pigmentation, freckles etc., agents having an activity inhibiting activity of tyrosinase (arbutin, licorice extract etc.), and reducing action of melanin Whitening agents such as drugs (ascorbic acid, etc.) that they have have been used.

[Problems to be Solved by the Invention]

Further, in recent years, attempts have been made to suppress pigment deposition by suppressing the transfer of melanosomes to keratinocytes by suppressing the formation of dendrites of melanocytes (Japanese Patent Laid-Open No. 10-287543). Open 20
01-199866, etc.). However, it has not been known so far that the yeast extract has the above-mentioned action and that the skin external preparation containing the yeast extract has an excellent whitening effect.

[0004]

Means for Solving the Problems As a result of intensive studies, the present inventors have found that a yeast extract has an inhibitory effect on melanocyte dendrite formation and is an excellent whitening ingredient. Then, it was found that this extract can be blended in a skin external preparation as a whitening and / or skin beautifying component, and by combining it with other medicinal components, a more excellent effect as a skin external preparation can be obtained, and the present invention has been completed. .

That is, the present invention provides a melanocyte dendrite formation inhibitor characterized by containing a yeast extract as an active ingredient. By containing this melanocyte dendrite formation inhibitor, It is intended to provide an external preparation for skin which is excellent in the whitening effect and the beautiful skin effect of preventing and improving pigmentation such as dullness of skin, spots, freckles, senile pigment spots or liver spots, and improving the transparency of the skin. The yeast extract according to the present invention is preferably an extract of wine yeast obtained during wine brewing.

The present invention also provides the following components (A) and (B) (A) a melanocyte dendrite formation inhibitor (B) according to claim 1 or 2, a whitening agent, an antioxidant and an anti-inflammatory agent. The present invention provides a skin external preparation characterized by containing one or more medicinal agents selected from a cell activating agent and an ultraviolet protective agent.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION Yeast used in the present invention is Sa
Sacchromyc belonging to the genus cchromyces
es cerevisiae or the like can be used.
The method for producing the yeast extract is not particularly limited, but, for example, a liquid obtained by autolyzing yeast or hydrolyzing it with a proteolytic enzyme or an acid or an alkali is centrifuged, and then a supernatant is obtained. Is obtained by filtration, concentration, or spray drying. The obtained extract is, if necessary, further purified by adsorption, decolorization, etc., using an ion exchange resin or the like, within a range that does not affect the effects of the present invention, in the form of a solution,
It can also be used in the form of paste, gel or powder.

The yeast extract of the present invention is preferably produced from wine yeast obtained during wine brewing. As an example, yeast at the end of wine brewing is collected, slurried and hydrolyzed, and then bacteria are crushed to crush the cells, and extraction is performed using, for example, water or hot water as an extraction solvent.
The obtained extract is centrifuged, the supernatant is microfiltered,
It can be obtained by performing a purification operation such as concentration under reduced pressure. In the extract of wine yeast thus obtained, proteins, amino acids, nucleic acids, which are components of the yeast itself,
In addition to containing vitamins and the like, it further contains useful components such as sugars, esters, lactones and higher alcohols produced during the wine brewing process. It is more preferable to use the extract of wine yeast obtained during wine brewing, from the viewpoint of suppressing melanocyte dendrite formation and exhibiting whitening and / or skin-beautifying effect when contained in a skin external preparation.

Examples of the extraction solvent that can be used in the production of the yeast extract of the present invention include water, lower monohydric alcohols (methyl alcohol, ethyl alcohol, propyl alcohol, butyl alcohol, etc.) and liquid polyhydric alcohols (propylene glycol). , Butylene glycol, glycerin, etc.), ethers (diethyl ether, tetrahydrofuran, etc.), ketones (acetone, etc.), and the like, and one kind or a mixture of two or more kinds thereof can be used.

The content of the yeast extract in the external preparation for skin of the present invention is preferably 0.00001 as a dry solid content.
˜5% by mass (hereinafter simply referred to as “%”), and more preferably 0.0001 to 2%. Within this range, the extract can be stably blended, the melanocyte dendrite formation can be effectively suppressed, and a high whitening and / or skin beautifying effect can be exhibited. When used as an extraction liquid, the concentration of the extraction liquid is not limited as long as the content of the dry solid as a solute is within the above range.

The yeast extract of the present invention can be formulated into a skin external preparation of various forms according to a conventional method as a whitening and skin-refining component by inhibiting the formation of melanocyte dendrites. By combining with other medicinal components, a skin external preparation having more excellent whitening and / or skin beautifying effect can be obtained.

In the present invention, yeast extract (component (A))
Other medicinal agents used in combination with (component (B))
Is selected from a whitening agent, an antioxidant, an anti-inflammatory agent, a cell activator, and an anti-UV agent, and specific medicinal agents include, for example, those shown below. Here, the "derivative" includes a formable ester or salt. In addition, in the parenthesis after the plant name, the alias of the plant, the herbal medicine name, etc. are described.

(Whitening Agent) As a whitening agent, vitamin C and its derivatives (dipalmitic acid-L-ascorbyl, tetraisopalmitic acid-L-ascorbyl etc. L-ascorbic acid alkyl ester, L-ascorbic acid phosphoric acid ester , L-ascorbic acid sulfate, etc.), placenta extract, glabridin, glabrene, liquiritin,
Isoliquiritin and licorice extract containing these, Yokuinin (pearl barley) extract, Scutellaria baicalensis (Ougon) extract, seaweed extract (kelp, macaw, wakame, hijiki, hibamatata, shimeji, torocombu, kajime, tsurame, chigaiso, hondawara, Brown algae such as giant kelp; red algae such as agar beetle, giant cucumber, giraffe, tsunomata, horse mackerel, Usbanori, Asakusanori, Matsunori, Tosakamatsu, Funori, gono-ori, kaimensou, igisu, ego-nori , Green algae such as kawaranori, marimo, cynomolgus, scorpionfish, futojuzumo, tamajuzumo, human aegusa, amidoro, etc .; cyanobacteria such as spirulina), sempukuka extract, grape extract, wheat extract, tomato Distillate, carotenoids (carotene, lycopene, astaxanthin, etc.), agarose,
Oligosaccharides, hydroquinone and its derivatives,
Cysteine and its derivatives, asparagus extract, ibukitoranoo extract, Neubara (Ageitsu) extract, eleuthero extract, pea extract, chamomile extract, cabbage extract, orange extract, raspberry extract,
Kiwi extract, Clara (kujin) extract, Coffee extract, Sesame oil, Perilla oil, Gokahi extract, Rice extract, Rice bran extract, Saishin extract, Hawthorn extract, Sunpen's (Kawaketsumei) extract, Peony extract ,
White lily extract, mulberry extract, Touki extract, beech extract, beech sprout extract, blackcurrant extract, hop extract, mica (Maikai, Hermanus) extract, mokka (bokeh) extract, Examples include Yukinoshita extract, tea extract (oolong tea, black tea, green tea, etc.), Reishi extract, soybean extract, molasses extract, Rakan fruit extract and the like.

Among these whitening agents, particularly preferable are vitamin C and its derivatives, licorice extract, Yokuinin (coix barley) extract, wheat extract, mulberry (sophoraceae) extract, seaweed extract, tea extract. Things can be mentioned.

(Antioxidant) As an antioxidant, vitamin E and its derivatives (dl-α (β, γ) -tocopherol, dl-α-tocopherol acetate, nicotinic acid-dl)
-Α-tocopherol, linoleic acid-dl-α-tocopherol, tocopherols such as dl-α-tocopherol succinate and its derivatives, ubiquinones, etc., vitamin A and its derivatives (retinol palmitate, retinol acetate, and the like) Derivatives, retinals such as dehydroretinal and their derivatives), carotenoids (carotene, lycopene, astaxanthin, etc.), quercetin, dibutylhydroxytoluene, butylhydroxyanisole, vitamin B and its derivatives (thiamine hydrochloride, thiamine sulfate, riboflavin, acetic acid) Riboflavin, pyridoxine hydrochloride, pyridoxine dioctanoate, flavin adenine dinucleotide, cyanocobalamin, folic acid, nicotinamide, nicotinic acid such as benzyl nicotinate , Etc.), vitamin C and derivatives thereof (dipalmitic acid-L-ascorbyl and tetraisopalmitic acid-L-ascorbyl etc., L-ascorbic acid alkyl esters, L-ascorbic acid phosphoric acid esters, L-ascorbic acid sulfuric acid esters Etc.), vitamin D
And its derivatives (ergocalciferol, cholecalciferol, dihydroxystanal, etc.), rutin, thiotaurine, taurine, hydroquinone and its derivatives, histidine, catechin and its derivatives, glabridine, grabrine, liquiritin, isoliquiritin and licorice extract containing these. Products, glutathione and its derivatives, gallic acid and its derivatives, cucumber extract,
Caykto extract, gentian (gentian) extract,
Genus ginger extract, cholesterol and its derivatives, hawthorn extract, peony extract, superoxide dismutase, ginkgo extract, Scutellaria baicalensis (Gougon) extract, carrot extract, Maikaika (Makai, Hermanus) extract, Sunpenz (Kawaratsukitsumei) extract Substance, Tormentilla extract, parsley extract, grape extract, peony extract, mannitol,
Mocca (bokeh) extract, Melissa extract, Yashajitsu (Yasha) extract, Yukinoshita extract, Rosemary (mannen wax) extract, Lettuce extract, Tea extract (Oolong tea, black tea, green tea, etc.), Seaweed extract, Examples include Ganoderma lucidum extract, eggshell membrane extract, placenta extract, Rakan fruit extract and the like.

Of these antioxidants, particularly preferred are vitamin E and its derivatives, vitamin C and its derivatives, rutin, yashajitsu extract, yukinoshita extract, mica squid extract, superoxide dismutase, ginkgo extract. , Glutathione and its derivatives, histidine, mannitol, and carotenoids.

(Anti-inflammatory agent) As an anti-inflammatory agent, glycyrrhizic acid and its derivative, glycyrrhetinic acid and its derivative, vitamin B and its derivative (thiamine hydrochloride, thiamine sulfate, riboflavin, riboflavin acetate, pyridoxine hydrochloride, pyridoxine diacid) Octanoate, flavin adenine dinucleotide, cyanocobalamin, folic acid, nicotinic acid amide, nicotinic acid such as benzyl nicotinate, choline, etc.), aloe extract, ashitaba extract, altea extract, arnica extract, sulfur and its Derivatives, nettle extract, Chinese mackerel extract, turmeric extract, yellowfin extract, Hypericum extract, chamomile extract, comfrey extract, honeysuckle extract, watercress extract , S Beer (Sage) extract, Waremoko (Jiuyu) extract, Perilla extract, Birch extract, Elderberry extract, Cattail (Physalis) extract, Mukuroji extract, Eucalyptus extract, Artemisia extract, Astragalus extract, Chondroitin Examples thereof include sulfuric acid and its derivatives, zinc oxide and the like.

Among these anti-inflammatory agents, glycyrrhizic acid and its derivatives, glycyrrhetinic acid and its derivatives, vitamins B and their derivatives are particularly preferable.

(Cell activating agent) As the cell activating agent, vitamin A and its derivatives (retinol palmitate, retinol acetate and other retinol and its derivatives, dehydroretinal and other retinal and its derivatives, etc.), carotenoids (carotene, lycopene, Astaxanthin, etc.), vitamin B and its derivatives (thiamine hydrochloride, thiamine sulfate, riboflavin, riboflavin acetate, pyridoxine hydrochloride, pyridoxine dioctanoate, flavin adenine dinucleotide, cyanocobalamin, folic acid, nicotinamide, benzyl nicotinate, etc. Nicotinic acid, choline, etc.), vitamin C and its derivatives (dipalmitic acid-L-ascorbyl and tetraisopalmitic acid-L-
L-ascorbic acid alkyl ester such as ascorbyl, L-ascorbic acid phosphoric acid ester, L-ascorbic acid sulfate, etc.), ribonucleic acid and its salt, deoxyribonucleic acid and its salt, α- and γ-linolenic acid, xanthine and its Derivatives (caffeine, etc.), almond extracts, asparagus extracts, amino acids and their derivatives (serine, glutamic acid, theanine, hydroxyproline, pyrrolidonecarboxylic acid, etc.), apricots (kyonin)
Extract, Ginkgo biloba extract, docosahexaenoic acid and its derivatives, eicosapentaenoic acid and its derivatives, yellowfin (Oafaku) extract, barley (Bakuga) extract, malt root extract, kiwi extract, cucumber extract, citric acid,
Lactic acid, malic acid, succinic acid, shiitake extract, horsetail extract, zebra extract, soybean extract, jujube extract, pepper extract, capsicum extract, tomato extract, garlic extract Substance, carrot extract, hinokitiol, butterbur extract, grape seed oil, beech extract, beech sprout extract, peach extract, eucalyptus extract, lily extract, lettuce extract, lemon extract, rosemary (mannen wax) ) An extract, a Reishi extract, etc. are mentioned.

Of these cell activating agents, particularly preferable ones are vitamin A and its derivatives, and vitamin C.
And its derivatives, vitamin B and its derivatives, citric acid, malic acid, pyrrolidonecarboxylic acid, and Reishi extract.

(Ultraviolet protection agent) As an ultraviolet protection agent,
2-Ethylhexyl paramethoxycinnamate, 4-te
rt-Butyl-4′-methoxydibenzoylmethane, oxybenzone and its derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, 2-hydroxy-4
-Methoxybenzophenone-5-sulfonate, etc.), titanium oxide, particulate titanium oxide, zinc oxide, particulate zinc oxide and the like. If the inorganic powder such as titanium oxide or zinc oxide is a fine particle, a higher effect is exhibited.

Among these ultraviolet ray inhibitors, particularly preferable are 2-ethylhexyl paramethoxycinnamate, titanium oxide, fine titanium oxide particles, zinc oxide and fine zinc oxide particles.

The compounding amount of the above-mentioned component (B) in the external preparation for skin of the present invention varies depending on the kind of the drug, but is preferably in the following range. Within this range, when combined with the yeast extract of component (A), there is no effect on the stability over time of the formulation and the yeast extract of component (A) in the formulation, and higher whitening and / or It can bring out the beautiful skin effect.

That is, the content of the whitening agent in the external preparation for skin of the present invention is preferably 0.00001 to 10%, more preferably 0.0001 to 5%. When the extract is used as it is, the dry solid content may be within this range. Within this range, a skin external preparation that exhibits a more excellent whitening and / or skin beautifying effect can be obtained.

The content of the antioxidant in the external preparation for skin of the present invention is preferably 0.00001 to 5%, more preferably 0.0001 to 3%. When the extract is used as it is, the dry solid content may be within this range. Within this range, a more excellent antioxidative effect is exhibited, and a skin external preparation having an excellent whitening and / or skin beautifying effect is obtained.

The compounding amount of the anti-inflammatory agent in the external preparation for skin of the present invention is preferably 0.00001 to 5%, more preferably 0.0001 to 3%.
When the plant extract is used as it is, the dry solid content may be within this range. Within this range, an external preparation for skin that exhibits an excellent anti-inflammatory effect and exhibits an excellent whitening and / or skin beautifying effect can be obtained.

The compounding amount of the cell activating agent in the external preparation for skin of the present invention is preferably 0.00001 to 5%, more preferably 0.0001 to 3%. When the extract is used as it is, the dry solid content may be within this range. Within this range, a skin external preparation exhibiting a more excellent effect of improving rough skin and exhibiting an excellent whitening and / or skin beautifying effect can be obtained.

The compounding amount of the ultraviolet protective agent in the external preparation for skin of the present invention is preferably 0.001 to 30%, more preferably 0.01 to 25%. Within this range, a skin external preparation that exhibits a more excellent anti-ultraviolet effect and exhibits an excellent whitening and / or skin beautifying effect can be obtained.

These whitening agents, antioxidants, anti-inflammatory agents, cell activators and UV inhibitors can be used alone or in combination of two or more.

The external preparation for skin in which the above-mentioned components (A) and (B) are combined can also be prepared as usual external preparations for skin in various forms according to a conventional method.

The form of the external preparation for skin of the present invention is not particularly limited, and examples thereof include emulsions, creams, lotions, packs, detergents, makeup cosmetics, dispersions, ointments, liquids, aerosols, patches and patches. It may be any form of cosmetics such as an agent, a liniment, etc., or an external medicine.

In addition to the above essential ingredients, the external preparation for skin of the present invention contains various components which are usually used in cosmetics, quasi drugs, external medicines, etc., that is, water, alcohols, oils, Surfactants, thickeners, powders, chelating agents, pH adjusting agents, various medicinal agents, extracts derived from animals and plants / microorganisms, fragrances and the like can be appropriately added within a range that does not impair the effects of the present invention. Of these, specific examples are shown below.

As alcohols, monohydric alcohols such as ethyl alcohol, glycerin, and 1, for the purpose of dissolution, refreshing feeling, antiseptic, moisturizing, etc., as long as they do not overlap with the essential components.
A polyhydric alcohol such as 3-butylene glycol can be used.

The oil agent can be used irrespective of its origin and properties in order to improve usability and usability.
For example, liquid paraffin, squalane, triglyceride oil, ester oil, waxes, fatty acids, higher alcohols, silicone oil, fluorinated oil, various waxes and the like.

The surface active agent is used for emulsifying or solubilizing an oil agent, and anionic, cationic, nonionic and amphoteric surface active agents can be used.

As the thickener, it is possible to use carboxyvinyl polymers, carrageenan, agar, xanthan gum, dextrin fatty acid esters, organically modified clay minerals, etc. regardless of whether they are chemically synthesized products or natural products. or,
These components can be used not only for adjusting the viscosity of the system, but also for gelling, moisturizing, film formation and the like.

The powder may be compounded or surface-treated in order to improve usability and usability regardless of shape, particle size, presence or absence of porosity, crystal structure and the like.
Inorganic powders such as talc, mica, sericite, silicic acid anhydride, organic powders such as nylon powder, fish scale foil, pearl pigments such as bismuth oxychloride, iron oxide, carbon black, inorganic pigments such as ultramarine, tar pigments and The lake, natural pigment, etc. are used depending on the application.

In order to prevent the quality deterioration of the components in the system, EDT
A chelating agent such as A and a pH adjusting agent with a buffer such as lactic acid-sodium lactate can also be used.

Examples of the medicinal agents include synthetic products and those derived from animals and plants / microorganisms. For example, as an antibacterial agent or bactericide, for the purpose of preventing and improving acne, benzoic acid, sodium benzoate, paraoxybenzoic acid ester, parachlorometacresol, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol and the like are used. To be By blending these, pigmentation due to bacterial skin inflammation such as acne is suppressed,
Further high whitening and / or beautiful skin effect can be exhibited.

The moisturizers include proteins or their derivatives or hydrolysates and their salts (collagen, elastin, keratin, etc.), mucopolysaccharides and their derivatives (hyaluronic acid), saccharides (sorbitol, erythritol, trehalose, inositol, Glucose, xylitol, sucrose and its derivatives, dextrin and its derivatives, honey, etc.), D-panthenol and its derivatives, glycolipids, ceramides, licorice extracts, avocado extracts, hot spring water, russia alba extracts, laurel extracts, Pleurotus cornucopia extract, Ononis extract, oat extract, quince seed extract, gardenia extract, kumazasa extract, grapefruit extract, burdock extract,
Cactus extract, Sophoraceae extract, Zygo extract, Spirea extract, Ginger extract, Ginger extract, Peppermint (peppermint) extract, Zenia mallow (Pleurotus cornucopiae) extract, Prunus chinensis extract, Prickly pear (thyme) Extract, camellia extract, Astragalus extract, Dokudami extract, peppermint extract, hamamelis extract, rose extract, cypress extract, sunflower extract, coltsfoot extract, butchers bloom extract, prune extract, loofah extract Substance, bodige extract, pine extract, quince extract, horse chestnut extract, mucin, cornflower extract, lime extract, lavender extract, apple extract, soybean and egg-derived phospholipids, urea and the like. Furthermore, as a moisturizing (emollient) agent by sealing the skin surface, jojoba oil, macadamia nut oil, olive oil, apricot kernel oil, persic oil, safflower oil, sunflower oil, avocado oil, medhome oil, camellia oil, almond oil, perilla oil. , Sesame oil, borage (borage) oil, cocoa butter, shea butter and the like. By blending these moisturizers, higher whitening and / or skin beautifying effects can be exhibited, and transparent skin can be realized.

As the blood circulation promoter, capsicum tincture, γ-oryzanol and the like are used for the purpose of promoting melanin excretion by promoting blood flow in the skin, and lipase, papain and the like are used as enzymes. By blending these, higher whitening and / or skin beautifying effect can be exhibited.

[0042]

EXAMPLES The present invention will be described in more detail with reference to Reference Examples, Test Examples and Examples, but the present invention is not limited thereto. Reference Example 1 Production of yeast extract Yeast (Saccharomyces) after completion of winemaking
cerevisiae) was taken out, washed, suspended in water as an extraction solvent and self-digested, and the obtained solution was filtered and freeze-dried. The lyophilized product was dissolved in water to give a yeast extract of 1%.

Test Example 1 Melanocyte dendrite formation inhibitory test Using the yeast extract produced in Reference Example 1 above, the melanocyte dendrite formation inhibitory action was investigated. That is, an appropriate amount of Dulbecco's modified MEM medium containing 10% FBS was added to a 6-well plate, and B16 melanoma cells derived from mouse were 4 ×.
10 ⁴ seeds, 37 ℃, carbon dioxide concentration 5vol%
I left it inside. After 2 days of culture, serum-free Dulbecco's modified M
The medium was replaced with EM medium, and α-MSH was added at a final concentration of 10 ^−7.
In addition, the yeast extract produced in Reference Example 1 had a content of 0 (control), 0.1, 0.
3 and 1.0 vol% were added and mixed. The next day, take a picture of the cell morphology with a phase contrast microscope (Olympus), count the number of dendrite-bearing cells (cells with dendrites longer than the diameter or short diameter of the cell), and retain dendrites. The cell number / total cell number was determined. The result is the measured value of the control is 1
The ratio is set to 00 and shown in Table 1.

(Result)

[Table 1]

As shown in Table 1, since the yeast extract of the present invention reduces the number of dendrite-bearing cells with respect to the total number of cells, it is clear that it is excellent in the effect of suppressing the formation of melanocyte dendrites. Became.

Example 1 Cream A cream was prepared by the composition shown in Table 2 and the following production method, and the yeast extract of the present invention alone or the yeast extract and a whitening agent, an antioxidant, an anti-inflammatory agent, a cell activator, and an ultraviolet ray preventer were used. The whitening / skinning effect of the combined use of the agents was examined. The results are also shown in Table 2.

(Composition and Results)

[Table 2]

(Production Method) A. Components (1)-(6), (13) and (14) are mixed and heated to maintain 70 ° C. B. Part of component (16) is heated and kept at 70 ° C. C. Add B to A and emulsify. D. The components (10), (12), (15) and (1 in C
After mixing (7) to (9) and (11) dissolved in the rest of 6), the mixture was cooled to obtain a cream.

(Test method) 27 per cream product tested
~ 15 women aged 54 years old will be on the panel every morning and evening.
After washing and washing the face, an appropriate amount of the test cream was applied to the face. The test was run for 12 weeks. The whitening and skin beautifying effects by application were evaluated according to the following criteria, and the evaluation results are shown in Table 2 according to the number of panels corresponding to each criterion.

(Evaluation criteria) <Evaluation> <Contents> Effective The dullness of the skin is no longer noticeable. Somewhat effective The dullness of the skin became less noticeable. Ineffective No change from before use.

Product 1 of the present invention containing the yeast extract of the present invention
It is clear that by applying these to the skin, it is possible to prevent and improve "dullness" of the skin, etc., and to make beautiful skin. Furthermore, yeast extract and whitening agents, antioxidants, By applying to the skin the products 2 to 7 of the present invention formulated by combining an anti-inflammatory agent, a cell activator, and an ultraviolet protective agent,
Prevents and improves skin "dullness", which is superior to the case of applying an external preparation containing yeast extract, a whitening agent, an antioxidant, an anti-inflammatory agent, a cell activator, and an anti-UV agent alone. It was clarified that the effects are synergistically exerted to make the skin beautiful.

[0052] Example 2 Face wash (Component) (%) (1) N-coconut oil fatty acid acyl-L-       Glutamic acid triethanolamine 20.0 (2) Lauryl dimethylamino acetic acid betaine 10.0 (3) Coconut oil fatty acid diethanolamide 3.0 (4) Palm oil fatty acid potassium 5.0 (5) Stearic acid 2.0 (6) Glycerin 20.0 (7) Polyethylene glycol 400 5.0 (8) Erythritol 2.0 (9) Propylene glycol 10.0 (10) Preservative suitable amount (11) Perfume proper amount (12) Yeast extract * 1 1.0 (13) Wheat germ extract * 2 0.02 (14) Dipotassium glycyrrhizinate * 3 0.1 (15) Yukinoshita extract * 4 0.02 (16) Purified water Remaining amount * 1 Manufactured in Reference Example 1 * 2 Made by Seiwa Kasei Co., Ltd. * 3 Made by Maruzen Pharmaceutical Co., Ltd. * 4 Made by Maruzen Pharmaceutical Co., Ltd.

(Manufacturing Method) A. The components (1) to (10) and (16) are heated and mixed,
Keep at 70 ° C. B. A was cooled to room temperature and components (11) to (15) were added and mixed to obtain a face wash.

[0054] Example 3 Gel cosmetic (Component) (%) (1) Methylcellulose 2.0 (2) Xanthan gum 1.0 (3) Sodium alginate 0.2 (4) Alkyl-modified carboxyvinyl polymer 0.2 (5) Sodium hyaluronate 1% aqueous solution 2.0 (6) Glycerin 10.0 (7) Polyethylene glycol 20000 1.0 (8) Methyl glucose 2.0 (9) Hydrogenated egg yolk phospholipid 0.2 (10) Phytosterol 0.1 (11) Sodium hydroxide 0.1 (12) Preservative appropriate amount (13) Perfume proper amount (14) Yeast extract * 1 0.001 (15) Green tea extract * 2 0.3 (16) Seaweed extract * 3 0.3 (17) Chamomile extract * 4 0.3 (18) Rosemary extract * 5 0.3 (19) Remaining amount of purified water * 1 Manufactured in Reference Example 1 * 2 Made by Maruzen Pharmaceutical Co., Ltd. * 3 Made by Maruzen Pharmaceutical Co., Ltd. * 4 Made by Maruzen Pharmaceutical Co., Ltd. * 5 Made by Maruzen Pharmaceutical Co., Ltd.

(Manufacturing Method) A. The components (1) to (4) and (19) are mixed by heating and cooled to room temperature. B. Ingredients (6) to (12) obtained by heating and mixing A at 70 ° C.
And mix. C. Ingredients (5) and (13) to (18) were added to B and mixed to obtain a gel cosmetic.

[0056] Example 4 Emulsion (Component) (%) (1) Hydrogenated soybean phospholipid 3.0 (2) Cholesterol 0.2 (3) Polyoxyethylene (5) cetyl ether 0.2 (4) Polyoxyethylene (10) hydrogenated castor oil 1.0 (5) cetostearyl alcohol 2.0 (6) Squalane 5.0 (7) Dipropylene glycol 7.0 (8) 1,3-butylene glycol 5.0 (9) L-serine * 1 0.2 (10) Sodium pyrrolidonecarboxylate * 2 0.1 (11) Trimethylglycine 2.0 (12) Methylcellulose 0.1 (13) Carboxyvinyl polymer 0.2 (14) Potassium hydroxide 0.1 (15) Preservative Suitable amount (16) Perfume proper amount (17) Yeast extract * 3 0.1 (18) Quinceseed extract * 4 5.0 (19) Neurose extract * 5 0.2 (20) Yokuinin extract * 6 0.2 (21) Altea extract * 7 0.2 (22) Purified water Remaining amount * 1 Made by Ajinomoto Co. * 2 Made by Ajinomoto * 3 Manufactured in Reference Example 1 * 4 Made by Koei Kogyo Co., Ltd. * 5 Made by Maruzen Pharmaceutical Co., Ltd. * 6 Made by Maruzen Pharmaceutical Co., Ltd. * 7 Made by Maruzen Pharmaceutical Co., Ltd.

(Manufacturing Method) A. The components (1) to (8) are heated and mixed to 70 ° C. B. A was heated and mixed at 70 ° C. (9) to (13),
(22) is added and mixed and cooled. C. Components (14) and (15) to (21) were added and mixed to B to obtain an emulsion.

[0058] Example 5 Lotion (Component) (%) (1) Macadamian nut oil 0.01 (2) Borage oil 0.01 (3) Cetyl octanoate 0.01 (4) Glyceryl tri-2-ethylhexanoate 0.01 (5) Acetic acid dl-α-tocopherol * 1 0.05 (6) Sorbitan sesquioleate 0.1 (7) Polyoxyethylene monooleate (20) sorbitan 0.1 (8) Polyoxyethylene (8) alkyl ether phosphoric acid 0.2 (9) Ethanol 10.0 (10) Sorbitol (70% aqueous solution) 5.0 (11) Glycerin 1.0 (12) 2-hydroxy-4-methoxybenzophenone         -5 Sodium sulfonate * 2 0.2 (13) Lactic acid (50% aqueous solution) 0.1 (14) Sodium lactate (50% aqueous solution) 0.3 (15) Preservative Suitable amount (16) Perfume proper amount (17) Yeast extract * 3 0.01 (18) Licorice extract * 4 0.1 (19) White lily extract * 5 0.05 (20) Perilla extract * 6 0.05 (21) Mikaika extract * 7 0.05 (22) Carrot extract * 8 0.05 (23) Purified water Remaining amount * 1 Made by Eisai * 2 Made by Shonan Chemical Industry Co., Ltd. * 3 Manufactured in Reference Example 1 * 4 Made by Maruzen Pharmaceutical Co., Ltd. * 5 Made by Maruzen Pharmaceutical Co., Ltd. * 6 Made by Maruzen Pharmaceutical Co., Ltd. * 7 Made by Maruzen Pharmaceutical Co., Ltd. * 8 Made by Maruzen Pharmaceutical Co., Ltd.

(Manufacturing Method) A. Components (1) to (9) are mixed and dissolved. B. A was added to and mixed with the dissolved components (10) to (23) to obtain a lotion.

Example 6 Sunscreen Emulsion (Component) (%) (1) Neopentyl glycol dicaprate 10.0 (2) 2-Ethylhexyl paramethoxycinnamate * 1 5.0 (3) Octamethylcyclotetra Siloxane 10.0 (4) Decamethylcyclopentasiloxane 10.0 (5) Dimethylpolysiloxane (20 mm ² / s) 5.0 (6) Fine particle titanium oxide 10.0 (7) Fine particle zinc oxide 5.0 (8 ) Polyoxyalkylene-modified organopolysiloxane 5.0 (9) Nylon powder 2.0 (10) Polyethylene powder 1.0 (11) Glycerin 5.0 (12) Ethanol 5.0 (13) Preservative Suitable amount (14) Fragrance Suitable amount (15) Yeast extract * 2 0.2 (16) Beech sprout extract * 3 0.1 (17) Peony extract * 4 0. (18) Waremoko extract * 5 0.01 (19) Green tea extract * 6 0.01 (20) Melissa extract * 7 0.01 (21) Gentian extract * 8 0.01 (22) Purified water residue Amount * 1 Made by BASF * 2 Made in Reference Example 1 * 3 Made by Gate Jose * 4 Made by Inabata Koryo * 5 Made by Maruzen Pharmaceutical * 6 Made by Maruzen Pharmaceutical * 7 Made by Maruzen Pharmaceutical * 8 Made by Maruzen Pharmaceutical

(Manufacturing Method) A. Components (1) to (13) are mixed at 70 ° C. and dispersed in a slurry. B. The component (22) heated to 70 ° C. was added to A to emulsify, and after cooling, the components (14) to (21) were added and mixed to obtain a sunscreen emulsion.

[0062] Example 7 Suncut (registered trademark) cream (water-in-oil type sunscreen) (Component) (%) (1) Polyoxyalkylene-modified organopolysiloxane 2.0 (2) Octyl palmitate 15.0 (3) Decamethylcyclopentasiloxane 20.0 (4) Glyceryl tribehenate 1.0 (5) Fine particle zinc oxide 12.0 (6) Fine particle titanium oxide 3.0 (7) Octyl methoxycinnamate * 1 7.0 (8) 4-tert-butyl-4'-       Methoxydibenzoylmethane * 2 1.0 (9) Safflower oil * 3 0.5 (10) Dipropylene glycol 5.0 (11) Ethanol 5.0 (12) Polyethylene powder 3.0 (13) Preservative suitable amount (14) Perfume proper amount (15) Yeast extract * 4 0.2 (16) Birch extract * 5 0.2 (17) Artemisia extract * 6 0.2 (18) Mannitol * 7 0.05 (19) Lime extract * 8 0.2 (20) Purified water Remaining amount * 1 Made by BASF * 2 Made by Givaudan * 3 Made by Ajinomoto * 4 Manufactured in Reference Example 1 * 5 Made by Maruzen Pharmaceutical Co., Ltd. * 6 Made by Maruzen Pharmaceutical Co., Ltd. * 7 Wako Pure Chemical Industries * 8 Made by Maruzen Pharmaceutical Co., Ltd.

(Manufacturing Method) A. The components (1) to (9) are mixed by heating. B. Ingredients (10) to (2
0) was added and mixed to obtain a sun-cut (registered trademark) cream (water-in-oil type sunscreen).

[0064] Example 8 Serum (Component) (%) (1) Polyoxyethylene isostearate (50) hydrogenated castor oil 0.2 (2) Hydrogenated soybean phospholipid 0.5 (3) Glycerin 7.0 (4) dl-α-tocopherol * 1 0.3 (5) Licorice extract * 2 0.2 (6) Cholesterol 0.1 (7) Ethanol 6.0 (8) 2-hydroxy-4-methoxybenzophenone       Sulfonic acid-2-sodium * 3 0.2 (9) L-ascorbic acid magnesium phosphate 0.5 (10) Citric acid 0.01 (11) Sodium citrate 0.1 (12) Xanthan gum 0.1 (13) Methylcellulose 0.1 (14) Seaweed extract * 4 0.1 (15) Gokahi extract * 5 0.1 (16) Soybean extract * 6 0.1 (17) Scutellaria extract * 7 0.1 (18) Nicotinic acid amide * 8 0.05 (19) Oat extract * 9 0.1 (20) Tomato extract * 10 0.1 (21) Sohakuhi extract * 11 0.1 (22) Preservative appropriate amount (23) Perfume proper amount (24) Yeast extract * 12 1.0 (25) Purified water Remaining amount * 1 Made by Eisai * 2 Made by Maruzen Pharmaceutical Co., Ltd. * 3 Made by Shonan Chemical Industry Co., Ltd. * 4 Made by Maruzen Pharmaceutical Co., Ltd. * 5 Made by Maruzen Pharmaceutical Co., Ltd. * 6 Made by Maruzen Pharmaceutical Co., Ltd. * 7 Made by Ichimaru Falcos * 8 Wako Pure Chemical Industries * 9 Made by Maruzen Pharmaceutical Co., Ltd. * 10 Made by Esperis * 11 Maruzen Pharmaceutical Co., Ltd. * 12 Manufactured in Reference Example 1

(Manufacturing Method) A. Components (1) to (7) are mixed and dissolved. B. The components (8) to (13) and (25) are heated and mixed, and cooled to room temperature. C. A and the components (14) to (24) were added and mixed to B to obtain a serum.

Example 9 Cream (ingredient) (%) (1) cetostearyl alcohol 3.0 (2) glycerin fatty acid ester 2.0 (3) polyoxyethylene monooleate (20) sorbitan 1.0 (4) Sorbitan monostearate 1.0 (5) N-stearoyl-N-methyltaurine sodium 0.5 (6) Vaseline 5.0 (7) Methylpolysiloxane (100 mm ² / s) 3.0 (8) Tri-2 -Glyceryl ethylhexanoate 20.0 (9) dl-α-tocopherol * 1 1.0 (10) Stearyl glycyrrhetinate * 2 0.1 (11) Titanium oxide 1.0 (12) Dipropylene glycol 10.0 ( 13) L-ascorbate magnesium phosphate 3.0 (14) sodium citrate 0.5 (15) lactic acid (50% water soluble Liquid) 0.1 (16) Yeast extract * 3 0.1 (17) Eleuthero extract * 4 0.3 (18) Hawthorn extract * 5 0.02 (19) King ginkgo extract * 6 0.02 ( 20) Hop extract * 7 0.02 (21) Raspberry extract * 8 0.02 (22) Touki extract * 9 0.02 (23) Disodium edetate 0.03 (24) Preservative Suitable amount (25 ) Fragrance Suitable amount (26) Yeast extract * 10 0.5 (27) Purified water Remainder * 1 Eisai * 2 Maruzen Pharmaceutical * 3 Maruzen Pharmaceutical * 4 Maruzen Pharmaceutical * 5 Maruzen Pharmaceutical * 6 Made by Inabata Fragrance Company * 7 Made by Maruzen Pharmaceutical Company * 8 Made by Esperis Company * 9 Made by Maruzen Pharmaceutical Company * 10 Manufactured in Reference Example 1

(Manufacturing Method) A. The components (1) to (10) are heated and mixed at 70 ° C. B. A heated and mixed with A at 70 ° C. (11) to (13),
Add (27) and mix. C. (14) to (26) were added to B and mixed, and cooled to room temperature to obtain a cream.

[0068] Example 10 Pack (Component) (%) (1) Polyvinyl alcohol 15.0 (2) Glycerin 10.0 (3) Polyoxyethylene (10) methyl glucose 3.0 (4) Glyceryl trioctanoate 5.0 (5) Polyoxyethylene alkyl ether sodium phosphate 1.0 (6) Ethanol 20.0 (7) Kaolin 2.0 (8) Titanium oxide 2.0 (9) Arnica extract * 1 0.1 (10) Aloe extract * 2 0.1 (11) Inchinko extract * 3 0.1 (12) Mokka extract * 4 0.1 (13) Rice bran extract * 5 0.01 (14) Hypericum extract * 6 0.01 (15) Rubus extract * 7 0.01 (16) Shobu extract * 8 0.01 (17) Grape extract * 9 0.01 (18) Lactic acid (50% aqueous solution) 0.5 (19) Sodium lactate (50% aqueous solution) 0.5 (20) Preservative suitable amount (21) Perfume proper amount (22) Yeast extract * 10 0.02 (23) Purified water Remaining amount * 1 Made by Maruzen Pharmaceutical Co., Ltd. * 2 Made by Maruzen Pharmaceutical Co., Ltd. * 3 Made by Maruzen Pharmaceutical Co., Ltd. * 4 Made by Maruzen Pharmaceutical Co., Ltd. * 5 Made by Koei Kogyo Co., Ltd. * 6 Made by Maruzen Pharmaceutical Co., Ltd. * 7 Made by Esperis * 8 Made by Maruzen Pharmaceutical Co., Ltd. * 9 Made by Ichimaru Falcos * 10 Manufactured in Reference Example 1

(Manufacturing Method) A. The components (1) to (5) and (23) are heated and mixed at 70 ° C. and cooled to room temperature. B. Components (6) to (22) were added to A and mixed to obtain a pack.

Each of the various types of external preparations for skin of Examples 3 to 10 had excellent stability over time, and when applied to the skin, exhibited excellent whitening and / or skin beautifying effects.

[0071]

INDUSTRIAL APPLICABILITY As described above, the yeast extract of the present invention is excellent in the melanocyte dendrite formation inhibitory action, and the skin external preparation containing it exhibits a high inhibitory effect on pigmentation, Blackening of the skin due to dullness, sunburn, etc., stains,
It is effective for prevention and improvement of freckles. In addition, the yeast extract of the present invention, a skin whitening agent, an antioxidant, an anti-inflammatory agent, a cell activating agent, an external skin preparation of the present invention in which the other pharmaceutical ingredients such as an anti-ultraviolet agent are used in combination are It has a superior whitening and / or skin beautifying effect as compared with the case where the extract or another medicinal component is blended alone. Therefore, the melanocyte dendrite formation inhibitor of the present invention and the skin external preparation containing the same can be advantageously used as cosmetics and external medicines for whitening and / or skin beautification.

Continued front page    F-term (reference) 4C083 AA031 AA032 AA082 AA112                       AA122 AB032 AB212 AB242                       AB432 AC012 AC022 AC072                       AC102 AC122 AC132 AC182                       AC212 AC242 AC302 AC312                       AC342 AC352 AC392 AC432                       AC442 AC532 AC582 AC642                       AC662 AC712 AC792 AC852                       AC902 AD022 AD042 AD072                       AD092 AD112 AD152 AD162                       AD172 AD202 AD262 AD302                       AD332 AD352 AD492 AD532                       AD572 AD642 AD662 BB46                       BB47 BB51 CC02 CC04 CC07                       DD31 DD41 EE10 EE12 EE16                       EE17                 4C087 AA01 AA02 BC90 CA11 MA17                       MA28 MA63 NA14 ZA89

Claims (4)

[Claims] 1. A melanocyte dendrite formation inhibitor comprising a yeast extract as an active ingredient. 2. The melanocyte dendrite formation inhibitor according to claim 1, wherein the yeast extract is an extract of wine yeast obtained during winemaking. 3. A skin external preparation for inhibiting melanocyte dendrite formation, which comprises the melanocyte dendrite formation inhibitor according to claim 1 or 2. 4. The following components (A) and (B) (A) The melanocyte dendrite formation inhibitor (B) whitening agent, antioxidant, anti-inflammatory agent, cell activating agent according to claim 1 or 2. And an external preparation for skin, which contains one or more kinds of medicinal agents selected from an ultraviolet protective agent.