KR100805386B1 - A composition comprising the fruit extract of platycarya strobilacea having anti-aging activity - Google Patents

Abstract

A composition comprises an extract of Platycarya strobilacea fruits is provided to be able to inhibit elastase and the MMP-1 expression, improving the skin elasticity and skin state, thereby being usefully used as a pharmaceutical composition and a cosmetic composition for inhibiting wrinkle formation and alleviating wrinkles. A skin external pharmaceutical composition comprises a crude extract of Platycarya strobilacea fruits which is extracted by using water including purified water or C1-4 lower alcohol. A skin external pharmaceutical composition for improving wrinkles comprises a polar solvent soluble extract of the Platycarya strobilacea fruits which is extracted by a solvent selected from the group consisting of water, methanol, butanol and a mixture solvent thereof. The composition is in the form of cream, gel, patch, sprayer, ointment, adhesive plaster, lotion, liniment, paste, or cataplasma.

Description

 A composition comprising the fruit extract of Platycarya strobilacea having anti-aging activity}

Aging of the skin can take many forms, including increased wrinkles, decreased elasticity and pigmentation. The most representative phenomenon of aging is wrinkles. The causes of aging of the skin to create such wrinkles can be divided into two major. Aging is caused by internal and external factors, which are natural aging with age. External factors include ultraviolet light, pollution, illness, drinking and smoking, and internal factors include free radicals and inflammation, stress, decreased cellular activity and mutation, and metabolic imbalance (Peter T., et al. , Physiology Of The Skin, Allured Publishing Corporation, pp. 61-72).

According to the structure of the skin to explain the cause of wrinkles in more detail, the skin is largely divided into the epidermis and the dermis. The cause of wrinkles in the epidermis is the reduction of the turn-over of keratinocytes, which leads to the accumulation of the stratum corneum, thereby thickening the stratum corneum, reducing the water-containing capacity of the stratum corneum, and causing the stratum corneum to harden and wrinkles. Done. The epidermal and dermal borders are involved in epidermal cell support, adhesion, transport of nutrients, and regulation of epidermal differentiation.As a result of various aging factors, collagen IV and VII are reduced due to UV, thereby reducing epidermal and dermal support functions. As a result, the selective permeation function is weakened and harmful ingredients easily affect the dermis, resulting in wrinkles (Keene, J. Cell Biol ., 104 , pp 611-621, 1987). The outermost layer of skin, the epidermal layer, is produced from the dermal layer, which consists of collagen and elastin. The moisturizing and elasticity of the skin are the two components of the dermis. Collagenase, an enzyme that breaks down collagen, and elastase, an enzyme that breaks down elastin, become active and the skin loses its elasticity and wrinkles (Herrmann). G. et al., Journal of Investigative Dermatology , 107 (3) , pp 92, 1996; Brenneisen P. et.al., Photochemistry & Photobiology , 64 (5) , pp 649, 1996). In addition, Matrix Metallo-Proteinase (MMP) -1 is a metalloproteinase having zinc in its active center and is secreted in the form of latent protease (zymogen) in vivo. In order to have enzymatic activity, structural modifications occur, resulting in cleavage and activation of amino terminal sites, and activated MMPs are regulated by inhibitors such as 2-macroglobulin or TIMPs (tissue inhibitors of metalloproteinase), and keratinocytes in the skin. (keratinocyte), the vast majority of cells, including fibroblasts secrete MMP (Fisher, GJ et al., Photochem . Photobiol . 69 , 154 , 1999). Even in one UV irradiation, MMP activity in the skin is increased, and the collagen in the skin is significantly disrupted, so that MMPs affect collagen breakdown of the dermis and play a very important role in light aging.

Oysterwood ( Platycarya strobilacea ) is distributed in Korea ( Southern Chungcheongnam-do), Japan, Taiwan, and China. It grows in sunny areas at the foot of the mountain or in the coastal Aqueous Rocks. It is 5-20m high and 10-50cm high. The leaf is odd leaf and composed of 7-19 small leaves without petioles. The leaf is elliptical or oval shaped with sharp tip and deep jagged teeth on the edge. White hairs on both sides of the leaf and peduncle but gradually disappear. Yellow-green flowers bloom in May-June, with male flower spikes 5-8cm and female flower spikes 2-4cm. Mature female spikelets are pine cone-shaped, fruit spikes are long oval, blackish brown, hairless. The shells do not fall off and are lanceolate. Fruits are nuts with wings and ripen in September-October. The main ingredient and efficacy is that the skin has eugloons, and the insecticides and bactericidal effects are strong and crushed, which is used for the treatment of acute suppuration of the skin or swelling of the head. Fruit has tannins, and in oriental medicine, the fruit is called a potentaceae, and it has the effects of analgesic, swelling, and gust wind, and it treats muscle pain, abdominal pain, toothache and eczema swelling. Used for Bark is used as a hair dye, and the leaves are toxic, so they are prohibited.

 In view of the above, the present invention screens a substance effective for preventing wrinkles among various natural substances in nature in order to find a substance that is effective in suppressing and improving skin wrinkles, and thus, the components extracted and fractionated from the oyster tree fruit are very excellent. It was found to exhibit the efficacy of suppressing wrinkle formation. In addition, it was found that even in the experimental results of applying a certain concentration in the cosmetic composition directly applied to the human skin exhibited the effect of improving the skin condition due to a pronounced wrinkle reduction and aging, to complete the present invention.

It is an object of the present invention to provide an external skin pharmaceutical composition and cosmetic composition containing the oyster bark fruit extract, the polar solvent soluble extract and the non-polar solvent soluble extract, which have an anti-aging and anti-wrinkle effect.

       In order to achieve the above object, the present invention provides a skin external pharmaceutical composition exhibiting the skin aging prevention and wrinkle relief effect containing crude oyster extract, polar solvent soluble extract and non-polar solvent soluble extract as an active ingredient.

       In another aspect, the present invention provides a cosmetic composition showing the skin aging prevention and anti-wrinkle effect containing the crude extract of oyster tree fruit, polar solvent soluble extract and non-polar solvent soluble extract as an active ingredient.

       Crude extracts as defined herein include water, including purified water, lower alcohols having 1 to 4 carbon atoms or extracts soluble in one or more solvents selected from the group consisting of propylene glycol, ethyl acetate, butylene glycol, ether, chloroform.

      In addition, the polar solvent soluble extract as defined herein is a solvent selected from water, methanol, butanol or a mixed solvent thereof, preferably an soluble extract in ethanol, and the non-polar solvent soluble extract is hexane, chloroform, methylene chloride, ethyl acetate, It means an extract available in a nonpolar solvent such as glycerin, butylene glycol.

      Hereinafter, a method of obtaining the crude extract, the polar solvent soluble extract and the nonpolar solvent soluble extract of the present invention will be described in detail.

The crude extract of the present invention is dried 1 to 30 times the volume of one or more solvents selected from the group consisting of water, ethanol, methanol, hexane, butylene glycol, propylene glycol and glycerin, including purified water as dried solvents The amount is preferably 10 to 20 times the volume of 1 to 30 hours, preferably at an extraction temperature of 30 to 100 ° C, preferably 50 to 95 ° C, with a cooling condenser installed to prevent the solvent from evaporating. Extraction method such as cold sediment extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, heating extraction for 3 to 20 hours, preferably 1 to 7 times, preferably 1 to 3 times extraction by hot water extraction method, filtered and concentrated under reduced pressure Freeze drying or spray drying to obtain a dry extract.

       In addition, the polar solvent and the non-polar solvent soluble extract of the present invention is obtained by the crude extract of the oyster bark fruit, preferably 2 to 7 times the weight of the ethanol soluble crude extract of the oyster tree fruit, preferably 4 to 6 times the volume of water After dispersing, hexane, chloroform, methylene chloride, ethyl acetate and butanol were sequentially added in the same volume with water, and fractionated 1 to 5 times, preferably 2 to 4 times, and the polar solvent and the nonpolar solvent of the present invention. Soluble extracts can be obtained.

      The composition of the present invention comprises the extract as a weight of solids, preferably in a weight ratio of 0.1 to 50%, more preferably in a weight ratio of 0.1 to 40%, most preferably 0.1 to 30% by weight, based on the total weight. .

      The crude extract, polar solvent soluble extract, and non-polar solvent soluble extract showed excellent effects as a result of confirming the elastase inhibitory effect, MMP-1 gene expression inhibitory effect, skin wrinkle improvement and skin elasticity enhancing effect, and also when applied to the skin. It was confirmed that there is no problem in safety.

      The present invention provides an external skin pharmaceutical composition for preventing skin aging and anti-wrinkle, containing the crude extract of oyster bark fruit, the polar solvent soluble extract and the non-polar solvent soluble extract obtained by the above method as an active ingredient.

       The external skin pharmaceutical composition containing the extract of the present invention as an active ingredient may further include appropriate carriers, excipients and diluents commonly used in the preparation of pharmaceutical compositions.

       The external skin pharmaceutical composition containing the extract according to the present invention as an active ingredient is formulated in the form of external preparations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, and sterile injectable solutions, respectively, according to a conventional method. It can be used in combination, preferably provides an external skin pharmaceutical composition of creams, gels, patches, sprays, ointments, warnings, lotions, linings, pasta or cataplasma.

       Carriers, excipients, and diluents that may be included in the composition containing the extract of the present invention as an active ingredient include lactose, dextrose, sucrose, oligosaccharide, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate , Gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose in the extract. ) Or lactose, gelatin and the like are mixed. In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Oral liquid preparations include suspending agents, liquid solutions, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

     Composition having an anti-aging and anti-wrinkle effect containing the extract of the present invention as an active ingredient is a skin external preparation formulation that can be applied to the skin cream, gel, patch, spray, ointment, warning agent, lotion agent, lining agent It may be used as a pharmaceutical composition in the form of external preparations for skin, pasta or cataplasma, but is not limited thereto.

      The preferred dosage of the composition containing the extract of the present invention as an active ingredient depends on the condition and weight of the patient, the extent of the disease, the drug form, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the preferred effect, the composition of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg per day. Topical administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

      In addition, the present invention provides a cosmetic composition having an anti-aging and anti-wrinkle effect of the oyster extract, polar solvent soluble extract and non-polar solvent soluble extract as an active ingredient.

      Extract of the present invention can be used in a variety of cosmetics and cleansing agents and the like having an anti-aging and wrinkle relief effect. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, skins, and the like, cleansing agents, face washes, soaps, treatments, and essences.

     Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

      The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl) Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

     The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

     The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

     The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

     In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

     Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

    Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoate cetostearyl, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate, tricitric acid Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

     Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

      Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

     Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

      Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

      Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

      As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

       As the nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

        Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

     As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, wuro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

     As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

    Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

    Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

     Examples of the alcohol include higher alcohols such as cetyl alcohol.

    Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5% weight percentage with respect to a total weight, More preferably 0.01-3% by weight.

    The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

   Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the compound as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

    The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

    Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

    When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

    When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

    When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

     When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

    When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

        Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples.

 However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Example  One. Oyster tree  Lychee Extract ( BIL -1) Preparation

200 g of completely dried oyster tree fruit (Gyeongdong Market, Seoul) was put in 1 kg of 70% ethanol, extracted by boiling in an extractor equipped with a cooling condenser for 3 hours, filtered through a 400 mesh filter cloth, cooled to room temperature, and filtered with a filter of Whatman 2 . The extract was filtered with a 0.45 μm filter and concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 50 g of extract (BIL-1).

Example  2. Oyster tree  Lychee Extract ( BIL -2) Preparation

Except for changing 70% ethanol to purified water, the same reaction process as in Example 1 was carried out to obtain 27.8 g of extract (BIL-2).

Example  3. Oyster tree  Lychee Extract ( BIL -3) Preparation

Except for changing 70% ethanol to 30% ethanol, the same reaction process as in Example 1 was carried out to obtain 29.5 g of extract (BIL-3).

Example  4. Oyster tree  Lychee Extract ( BIL -4) Preparation

Except for changing 70% ethanol to 30% glycerin, the same reaction process as in Example 1 was carried out to obtain 25.5 g of extract (BIL-4).

Example  5. Oyster tree  Lychee Extract ( BIL -5) Preparation

Except for changing 70% ethanol to 30% butylene glycol was carried out the same reaction process as in Example 1 to obtain 26.4g of extract (BIL-5).

Example  6. Oyster tree  Lychee Extract ( BIL -6) Preparation

Except for changing 70% ethanol to 80% methanol, the same reaction process as in Example 1 was performed, to obtain 32.7 g of extract (BIL-6).

Example  7. Oyster tree  Lychee Extract ( BIL -7) Preparation

Except for changing 70% ethanol to 80% acetone, the same reaction process as in Example 1 was carried out to obtain 28.8 g of extract (BIL-7).

Example  8. Oyster tree  Lychee Extract ( BIL -8) Preparation

Except for changing 70% ethanol to ethyl acetate, the same reaction process as in Example 1 was performed, to obtain 30.3 g of extract (BIL-8).

Example  9. Oyster tree  Lychee Extract ( BIL -9) Preparation

50 g of the reduced pressure concentrate of Example 1 was suspended in 1 kg of distilled water, shaken and extracted using 1 kg of hexane, and then fractionated and concentrated under reduced pressure to obtain 3.66 g of an extract (BIL-9).

Example  10. Oyster tree  Lychee Extract ( BIL -10) Preparation

The residual water layer of Example 9 was extracted with shaking with 1 kg of ethyl acetate, fractionated and concentrated under reduced pressure to obtain 8.3 g of the extract (BIL-10).

Example  11. Oyster tree  Lychee Extract ( BIL -11) Preparation

The residual water layer of Example 10 was shaken and extracted with 1 kg of n-butanol, fractionated, and concentrated under reduced pressure to obtain 12 g of an extract (BIL-11).

Example  12. Oyster tree  Lychee Extract ( BIL -12) Preparation

The residual water layer of Example 11 was concentrated under reduced pressure to obtain 21.3 g of extract (BIL-12).

Experimental Example  One. Elastase  Inhibitory effect measurement

In order to measure the elastase inhibitory effect of the oyster bark fruit extract of Examples 1 to 8 was tested as follows. (James AEK, Timothy DW and Gordon L. Biochemistry ., 35 , pp.9090-9096, 1996)

     The buffer solution was prepared by adjusting 0.267M Tris solution with 0.267M hydrochloric acid (HCl) solution to pH 8.0, and the elastase substrate was prepared with the Succ-Ala-Ala-Ala-p-nitroanilide standard at a concentration of 8.8 mM. The enzyme solution was prepared by using a Porcine Pancreatic Elastase standard product at a concentration of 10 µg / ml.

      Absorbance A was measured using 100 μl of purified water instead of the sample in the same manner as the test solution, and the absorbance A was measured. 120 μl of purified water was used instead of the enzyme solution and the sample solution. The absorbance D was measured.

     20 μl of the substrate solution and 100 μl of the sample in Example 1 were mixed with 60 μl of the buffer prepared in Experimental Example 1, and 20 μl of the enzyme solution was mixed and shaken to react at 25 ° C. for 15 minutes. The amount of p-nitroaniline was absorbed at 410 nm. B was measured. 20 μl of the substrate solution, 20 μl of purified water, and 100 μl of the sample were added to 60 μl of the buffer, and the absorbance C was measured using the solution obtained as the control solution.

    Elastase inhibition rate was calculated by the following equation 1, the results are shown in Table 1.

     % Elastase inhibition = [1-{(B-C) / (A-D)}] × 100

extract  % Elastase inhibition at 100 μg / ml BIL-1 69.7 BIL-2 40.8 BIL-3 47.9 BIL-4 45.5 BIL-5 40.6 BIL-6 38.9 BIL-7 41.2 BIL-8 37.7

As a result, it was confirmed that the elastase inhibition rate of the BIL-1 extract is excellent as shown in Table 1.

Experimental Example  2. Cytotoxicity Measurement

In order to measure the cytotoxicity of the oyster fruit extracts of Examples 1 to 12, fibroblasts (Human Skin fibroblast) were cultured to describe MTT [3- (4,5-dimethylthiazol-2-yl) -2,5 described in the literature. using -diphenyltetrazolium bromide reduction test] method (AA Van de Loosdrecht, E. Nennie , GJ Ossenkoppele, and RH Beelen, Langenhuijsen MM. J. Immunol. methods, 141 (1), pp.15, 1991) as follows Experiment.

      MTT assay is an assay used to measure cell proliferation and toxicity.The purple formazan derivative is insoluble in yellow water-soluble salt MTT by succinate dehydrogenase (called succinate dehydrogenase or mitochondrial dehydrogenase) in mitochondria of living cells. It is to use the principle of reducing to. The resulting formazan derivative was dissolved by adding a dissolving agent (usually using DMSO) and then measuring absorbance.

Human fibroblasts (ATCC, CRL-2706) were inoculated in 24-well culture plates at a concentration of 5 × 10 ⁴ cells / ml. The medium used was IMDM (Iscove's Modified Dulbecco's Medium; GIBCO, USA) containing 10% bovine serum. After 24 hours of inoculation, the cells were replaced with IMDM without the addition of bovine serum, and the samples of Examples 1 and 9 to 12 were added at different concentrations , and then cultured in a 37 ° C., 5% CO ₂ incubator for 3 days.

     The supernatants of the cells of Examples 9 to 12, which were fractionated from Experimental Example 1 and Example 1, were removed and washed again by adding 200 µl of 5% PBS (phosphate buffered saline). After adding MTT solution to 1 ml per well, MTT was removed after 4 hours, DMSO was added to 1 ml per well to dissolve the formazan derivative, and the absorbance was measured at 570 nm. Cell viability was calculated using Equation 2 below, and the results are shown in Table 3.

extract Cell survival rate (%) BIL-1 (5 μg / ml) 101.12 BIL-1 (10 μg / ml) 98.74 BIL-9 (5 μg / ml) 99.36 BIL-9 (10 μg / ml) 97.65 BIL-10 (1 μg / ml) 99.49 BIL-10 (5 μg / ml) 95.84 BIL-11 (1 μg / ml) 112.36 BIL-11 (5 μg / ml) 115.89 BIL-12 (1 μg / ml) 110.13 BIL-12 (5 μg / ml) 114.24

As a result, as shown in Table 3, the BIL-1, BIL-9, BIL-10, BIL-11 and BIL-12 extracts were found to be non-toxic to fibroblasts.

Experimental Example  3. MMP -One mRNA  Expression inhibition effect measurement

      Matrix Metallo-Protease-1, which promotes collagen breakdown in the skin, is promoted by UVA and RT-PCR was performed to see the effect of inhibiting gene expression of MMP-1.

Fibroblasts were aliquoted at a density of 1 × 10 ⁶ in 60 mm dishes and cultured at 37 ° C. for 24 hours. After irradiating 6J of UVA, Example 1 and Examples 9-12 were added according to concentration, and it was incubated at 37 degreeC for 24 hours.

      Cell medium was removed, and 1 ml of Trizol (Trizol, invitrogen, USA) was added and separated according to RNA separation method of Invitrogen. RNA was quantified at 260 nm using an ultraviolet detector, followed by reverse transcription-polymerase chain reaction. Reverse transcription polymerization reaction was used all-in-one reverse transcription polymerization kit (All-in-one RT-PCR kit, SuperBio, Korea), primers and reaction conditions are shown in Tables 4 and 5 below, in the manual of the all-in-one reverse transcription polymerization kit According to the experiment.

MMP-1 Primer Sense (SEQ ID NO: 1)  5'-TGGGAGCAAACACATCTGA-3 ' Antisense (SEQ ID NO: 2)  5'-ATCACTTCTCCCCGAATCGT-3 ' Reaction conditions Reverse transcription at 50 ° C for 30 minutes, then inactivate reverse transcription enzyme at 96 ° C for 3 minutes, polymerization at 25 cycles of 94 ° C for 1 minute, 48 ° C for 1 minute, and 72 ° C for 1 minute

Actin primer Sense (SEQ ID NO: 3)  5'-GAGACCTTCAACACCCCAGCC-3 ' Antisense (SEQ ID NO: 4)  5'-GGCCATCTCTTGCTCGAAGTC-3 ' Reaction conditions Reverse transcription at 50 ° C for 30 minutes, then inactivate reverse transcription enzyme at 96 ° C for 3 minutes, polymerization at 94 cycles of 1 minute, 62 ° C 1 minute, 72 ° C 1 minute and 25 cycles

 The results of measuring the inhibitory effect of MMP-1 expression were shown by correcting for actin, and as shown in Table 6, Examples 1, 11, and 12 show the activity of MMP-1 induced by UVA below the control level. It was confirmed to inhibit.

sample MMP-1 expression level (%, corrected for actin) Control 62.96 UVA 6J 100 UVA 6J + BIL-1 (5 μg / ml) 68.60 UVA 6J + BIL-1 (10 μg / ml) 52.39 UVA 6J + BIL-9 (5 μg / ml) 72.66 UVA 6J + BIL-9 (10 μg / ml) 81.37 UVA 6J + BIL-10 (1 μg / ml) 105.99 UVA 6J + BIL-10 (5 μg / ml) 85.96 UVA 6J + BIL-11 (1 μg / ml) 51.77 UVA 6J + BIL-11 (5 μg / ml) 60.83 UVA 6J + BIL-12 (1 μg / ml) 107.69 UVA 6J + BIL-12 (5 μg / ml) 66.19 UVA 6J + EGCG (25 μg / ml) 64.76

Examples of the preparation of the cosmetic composition comprising the oyster bark fruit extract, the polar solvent soluble extract, the non-polar solvent soluble extract of the present invention, but the present invention is not intended to limit, but is intended to explain in detail only.

Production Example  1. flexible lotion ( skin Manufacturing

Table 7 shows an example of the preparation of the flexible lotion (skin) in the cosmetic containing the crude extract of oyster tree.

number Raw material Formulation Example 1 Comparative Formulation Example 1 Control One   BIL-1 3.0 - - 2   BIL-12 - 3.0 - 3 glycerin 3.0 3.0 3.0 4 Butylene Glycol 2.0 2.0 2.0 5 Propylene glycol 2.0 2.0 2.0 6 Polyoxyethylene (60) Curing  Castor Oil 1.00 1.00 1.0 7 ethanol 10.0 10.0 10.0 8 Triethanolamine 0.1 0.1 0.1 9 antiseptic a very small amount a very small amount a very small amount 10 Pigment a very small amount a very small amount a very small amount 11 Spices a very small amount a very small amount a very small amount 12 Purified water Remaining amount Remaining amount Remaining amount

<Manufacturing method>

Formulation example  One

         3, 4, 5, and 9 were sequentially added to 12 and stirred to dissolve. Then, 6 was heated to 60 ° C for dissolution, followed by 11 times of stirring. Finally, 1 or 2, 7, 8, and 10 were added thereto, sufficiently stirred, and aged after passing through a microfluidizer.

Comparative formulation example  One

Except for raw material 1 was prepared in the same manner as in Formulation Example 1.

Production Example  2. Preparation of nutrient lotion (lotion)

Table 8 shows an example of preparation of nutrient cosmetic lotion (lotion) in cosmetics containing crude extract of oyster tree.

number Raw material Formulation Example 2 Comparative Formulation Example 2 Control One   BIL-1 3.0 - - 2   BIL-12 - 3.0 - 3 Cytostolol 1.70 1.70 1.70 4 Polyglyceryl 2-oleate 1.50 1.50 1.50 5 Ceteares-4 1.2 1.2 1.2 6 cholesterol 1.5 1.5 1.5 7 Dicetylphosphate 0.4 0.4 0.4 8 Concentrated glycerin 5.0 5.0 5.0 9 Sunflower oil 10.0 10.0 10.0 10 Carboxy Vinyl Polymer 0.2 0.2 0.2 11 Xanthan Gum 0.3 0.3 0.3 12 antiseptic a very small amount a very small amount a very small amount 13 Spices a very small amount a very small amount a very small amount 14 Purified water Remaining amount Remaining amount Remaining amount

<Manufacturing method>

Formulation example  2

   3, 4, 5, and 6 are homogenized at a constant temperature to refer to nonionic amphiphilic lipids. The nonionic amphiphilic lipids and raw materials 1 or 2 and 7, 8 and 14 are mixed and homogenized at a constant temperature and passed through a microfluidizer, followed by the slow addition of 9 at a constant temperature to homogenize again. Passed it through again. 10, 11, 12, and 13 were added and dispersed to stabilize and mature.

Comparative formulation example  2

Except for raw material 1 was prepared in the same manner as in Formulation Example 1.

Production Example  3. Preparation of nutrition cream

Table 9 shows an example of the preparation of the nourishing cream in the cosmetic containing the oyster extract.

number Raw material Formulation Example 3 Comparative Formulation Example 3 Control One   BIL-1 5.0 - - 2   BIL-12 - 5.0 - 3 Cytostolol 4.0 4.0 4.0 4 Polyglyceryl 2-oleate 3.0 3.0 3.0 5 Ceteares-4 2.0 2.0 2.0 6 cholesterol 3.0 3.0 3.0 7 Dicetylphosphate 0.4 0.4 0.4 8 Concentrated glycerin 5.0 5.0 5.0 9 Sunflower oil 22.0 22.0 22.0 10 Carboxy Vinyl Polymer 0.5 0.5 0.5 11 Triethanolamine 0.5 0.5 0.5 12 antiseptic a very small amount a very small amount a very small amount 13 Spices a very small amount a very small amount a very small amount 14 Purified water Remaining amount Remaining amount Remaining amount

<Manufacturing method>

Formulation example  3

  Raw materials 3, 4, 5, and 6 are homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipids and raw materials 1 or 2 and 7, 8 and 14 are mixed and homogenized at a constant temperature and passed through a microfluidizer, followed by the slow addition of 9 at a constant temperature to homogenize again. Passed it through again. 10, 11, 12, and 13 were added and dispersed to stabilize and mature.

Comparative formulation example  3

Except for raw material 1 was prepared in the same manner as in Formulation Example 1.

Production Example  4. Preparation of Essence

  Table 10 shows an example of the preparation of the essence in the cosmetic containing the crude extract of oyster tree.

number Raw material Formulation Example 4 Comparative Formulation Example 4 Control One   BIL-1 5.0 - - 2   BIL-12 - 5.0 - 3 Cytostolol 1.70 1.70 1.70 4 Polyglyceryl 2-oleate 1.50 1.50 1.50 5 Ceteares-4 2.0 2.0 2.0 6 cholesterol 3.0 3.0 3.0 7 Dicetylphosphate 0.4 0.4 0.4 8 Concentrated glycerin 5.0 5.0 5.0 9 Sunflower oil 22.0 22.0 22.0 10 Carboxy Vinyl Polymer 0.5 0.5 0.5 11 Triethanolamine 0.5 0.5 0.5 12 antiseptic a very small amount a very small amount a very small amount 13 Spices a very small amount a very small amount a very small amount 14 Purified water Remaining amount Remaining amount Remaining amount

<Manufacturing method>

Formulation example  4

    Raw materials 3, 4, 5, and 6 are homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid and raw materials 1 or 2 and 7, 8 and 14 are mixed and homogenized at a constant temperature and passed through a microfluidizer, and then the raw material 9 is gradually added at a constant temperature to homogenize and then again micro Passed through the fluidizer again. 10, 11, 12, and 13 were added and dispersed to stabilize and mature.

Comparative formulation example  4

Except for raw material 1 was prepared in the same manner as in Formulation Example 1.

Production Example  5. Manufacture of Foundation

Table 11 shows an example of the preparation of the foundation in the cosmetic containing the oyster extract.

number Raw material Formulation Example 5 Comparative Formulation Example 5 Control One   BIL-1 3.0 - - 2   BIL-12 - 3.0 - 3 Beeswax 2.0 2.0 2.0 4 Pyromethicone 2.0 2.0 2.0 5 Liquid paraffin 5.0 5.0 5.0 6 Squalane 5.0 5.0 5.0 7 Stearic acid 2.0 2.0 2.0 8 Lipophilic monostearic acid glycerin 3.0 3.0 3.0 9 Caprylic / Capric Triglycerides 4.0 4.0 4.0 10 glycerin 4.0 4.0 4.0 11 Propylene glycol 3.0 3.0 3.0 12 Butylene glycol 3.0 3.0 3.0 13 Triethanolamine 1.0 1.0 1.0 14 Aluminum Magnesium Silicate 0.5 0.5 0.5 15 Pigment 12 12 12 16 antiseptic a very small amount a very small amount a very small amount 17 Spices a very small amount a very small amount a very small amount 18 Purified water Remaining amount Remaining amount Remaining amount

<Manufacturing method>

Formulation example  5

   10, 11, 12, 13, 14, 15, 16 and 18 were mixed and stirred and heated to 80-85 ° C. and put into the manufacturing unit, followed by actuating emulsifier and 3, 4, 5, 6, 7, 8 and 9 The mixture was heated to 80-85 ° C. and emulsified. After emulsification, the mixture was cooled to 45 ° C. while stirring using a stirrer, and 17 times were added thereto. After cooling to 35 ° C., 1 or 2 times were added thereto, and cooled to 25 ° C. and aged.

Comparative formulation example  5

Except for raw material 1 was prepared in the same manner as in Formulation Example 1.

Production Example  6. Preparation of Hydrophilic Ointment

number Raw material Content (% by weight) Content (% by weight) One   BIL-1 0.5 - 2   BIL-12 - 0.5 3   White vaseline 250 250 4   Stearyl alcohol 220 220 5   Ethyl (or methyl) p-oxybenzoate 0.25 0.25 6   Propylene glycol 120 120 7   Sodium lauryl sulfate 15 15 8   Propyl p-oxybenzoate 0.15 0.15

clinical Test Example  1. Measurement of skin elasticity improvement effect by reducing wrinkle depth

Formulation Example 3 and Comparative Formulation Example 3 of Preparation Example 3 were divided into two groups of 20 women, and then applied twice daily in the morning and evening on the left eye edge to test the effect of the oyster tree fruit extract on the skin wrinkles. Nourishing cream of the control group was treated at the right eye edge. The skin wrinkle depth of the skin surface after 15 and 30 days of application was measured by Cutometer SEM 474. Skin elasticity measurement by Cutometer SEM 474 is a measure of the depth of wrinkles, the smaller the value is the better elasticity. The elasticity value was shown as a percentage decrease compared to the control, and the average value of 20 subjects was shown in the results. The results are shown in Table 12 below.

sample Skin elasticity (wrinkle depth reduction) effect (%)  0 days  15th  30 days Formulation Example 3  0.51  0.42  0.39 Comparative Formulation Example 3  0.53  0.43  0.40 Control  0.52  0.49  0.48

As a result, as shown in Table 12, it was confirmed that the skin elasticity effect in Formulation Example 3 and Comparative Formulation Example 3 is superior to the control.

clinical Test Example  2. Experiment to confirm skin condition improvement effect

     In order to confirm the effect of improving the skin-labeling skin condition on the essence of Formulation Example 4 and Comparative Formulation Example 4 of Preparation Example 4, the following clinical confirmation experiment was carried out. Healthy female aged 36-69 years old, divided into two groups of 30 people. The essence of Formulation 4 and Comparative Formulation 4 of Preparation Example 4 was found on the left side of the face and the essence of the control group on the right side of the face. After applying twice a day for 4 weeks, the degree of improvement of skin condition was measured to confirm the improvement of skin condition. Table 13 shows the results of (A) improving the elasticity and sagging of the skin and (B) improving the wrinkles and fine lines with the following scores. In the group using Formulation Example 4, a much better skin improvement effect was observed in comparison with the group using Comparative Example 4 in both the improvement effect on the elasticity and sagging of the skin and the improvement effect on the wrinkles and fine lines (see Table 14).

sample Skin elasticity and sagging Wrinkles and fine lines Formulation Example 4 65 61 Comparative Formulation Example 4 35 40

 As described above, the composition containing the crude oyster extract, polar solvent soluble extract and non-polar solvent soluble extract of the present invention as an active ingredient has the effect of inhibiting elastase, inhibiting MMP-1 expression, improving skin elasticity and It can be used as an external skin pharmaceutical composition and cosmetic composition for inhibiting wrinkles and alleviating wrinkles by exhibiting an improvement in skin condition.

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Claims (12)

     A skin external pharmaceutical composition for improving wrinkles, which comprises crude extract of oyster tree fruit extracted with water containing purified water or a lower alcohol solvent having 1 to 4 carbon atoms as an active ingredient. delete A skin external pharmaceutical composition for improving wrinkles, which comprises oyster bark fruit polar solvent soluble extract extracted with water, methanol, ethanol, butanol or a mixed solvent thereof as an active ingredient. delete A skin external pharmaceutical composition for improving wrinkles containing ethyl acetate soluble extract of oyster fruit as an active ingredient. delete The skin external pharmaceutical composition according to claim 1, 3 or 5, wherein the composition is a cream, gel, patch, spray, ointment, warning, lotion, linen, pasta or cataplasma formulation. A cosmetic composition comprising a polar solvent soluble extract or ethyl acetate soluble extract selected from water, methanol, ethanol, butanol, or a mixed solvent thereof, including purified water of an oyster tree fruit having an anti wrinkle effect as an active ingredient. delete delete delete According to claim 8, wherein the composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Cosmetic composition, characterized in that the formulation selected from the group consisting of pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.