KR20090097483A - Composition comprising the mixed extract comprising mylabris sidae fabricius showing reduced toxicity for treating and improving leukoplakia - Google Patents

Abstract

A composition containing complex herbal extracts is provided to ensure the non toxic effect and use for a pharmaceutical composition and cosmetic composition. A skin external pharmaceutical composition for treating leukoplakia comprises a complex herbal extracts containing Cantharis as an active ingredient. A method for producing the complex herbal extracts comprises: a first step of maturing Cantharis, leaves and barks of Morus alba L. with water or C1-C4 low alcohol such as ethanol or methanol; a second step of maturing leaves of bamboo with water or C1-C4 low alcohol such as ethanol or methanol; a third step of powder of mung bean in water, C1-C4 low alcohol; a fourth step of maturing powder of mung bean and talc; and a fifth step of extracting by stirring, heating, freezing, or ultra sonic wave, filtering mixture extract and concentrating or drying.

Description

Composition for the mixed extract comprising Mylabris sidae Fabricius showing reduced toxicity for treating and improving leukoplakia}

 The present invention relates to a composition for the treatment and amelioration of vitiligo, containing as an active ingredient, a half-myocardium-containing complex herbal extract with reduced toxicity.

Document 1 Kubo M. et al., Fragrance Journal , 8 , p.48, 1995

2 Seiji M et al., Ann . NY Acad . Sci ., 100 , p . 497, 1963

Marmol V. et al., FEBS Letter , 327 , p. 307, 1993

      [Reference 4] Herbology, Younglimsa, pp 646-647, 1991

Skin is a very important tissue that has direct contact with the external environment, protects the human body and has biochemical and physical functions. This tissue is largely divided into three parts: the epidermis, the dermis, and the hypodermis. Human skin color is determined by the amount of melanin, keratin and hemoglobin, of which melanin is the most decisive factor. Melanin is synthesized in melanocytes, pigment cells in the basal layer of the skin, and is transferred to the surrounding keratinocytes to give a human skin color (Kubo M. et al., Fragrance Journal , 8 , p. 48, 1995).

The abnormal production of melanin causes skin lesions such as vitiligo. On the contrary, it is overproduced by external environmental factors, causing pigmentation such as blemishes and freckles, and closely related to skin cancer. In particular, when skin cells are stimulated by ultraviolet rays, a signal for melanin pigment production is produced by tyrosinase, an important enzyme in melanin synthesis, and these have a direct or indirect effect on melanocytes, causing melanin pigment production (Seiji M). et al., Ann . NY Acad . Sci ., 100 , p. 497, 1963). This step can be inhibited by using tyrosinase substrate analogs. Recently, melanin is produced by tyrosinase as well as tyrosinase-related protein 1 (TRP-1) and tyrosinase-related protein 2 (TRP-2, Dopachrome tautomerase) as an important enzyme involved in the biosynthesis of melanin. New developments that can inhibit these enzymes may be a way to prevent skin pigmentation (Marmol V. et al., FEBS Letter , 327 , p. 307, 1993).

      Raw materials formulated in whitening compositions as inhibitors of conventional tyrosinase include plant extracts such as ascorbic acid (vitamin C) and its derivatives, lettuce extract, green tea extract, aloe extract, golden extract, as well as kojic acid, arbutin, Oil-soluble licorice extract; and the like. However, various plant extracts are unstable over time, the effect is not sustained when formulated into the product, and the inhibitory effect of tyrosinase activity is insignificant. In addition, kojic acid, arbutin, oil-soluble licorice extract is excellent in whitening effect, but the storage stability is not satisfactory over time, there is a limit to give a whitening effect as the amount is limited.

      Banmyo is a herb that uses medicinal materials, such as Epicauta gorhami Marseue, Lytta vesicaforia Latreille, Mylabris cichorii Linne and Mylabris phalerata Pallas, as medicinal parts. ) Is the main ingredient and contains fat and aromatic substances. This herbal medicine has strong toxicity during oral administration and is classified as a herbal medicine that is usually used only for external use. 646-647, 1991).

     Therefore, the present inventors have confirmed that the present invention by confirming the harmless and non-toxic effect to the human body than the existing composition for improving vitiligo through the method of producing a half-mycotic compound herbal extract reduced toxicity to eliminate the side effects due to toxicity, It was completed.

In order to achieve the above object, the present invention, after drying and cutting the half seedlings, upper leaves, baekryepi, about 20 to 80 times, preferably about 65 times the volume of water or C ₁ to C such as ethanol, methanol, etc. A first step of aging in a lower alcohol of ₄ , preferably ethanol at room temperature for 1 to 30 days, preferably about 7 days; Put the composite herbal extract obtained by aging in the first step into a dehydrator and extract only the solvent, preferably ethanol, and after drying and cutting the bamboo leaves, kill, about 20 to 80 times the weight of the herbal medicine, preferably about 65 times In a volume of water or C ₁ to C ₄ lower alcohols, such as ethanol, methanol, etc., preferably in ethanol for 1 to 30 days, preferably 5 to 15 days, 1 to 50 ℃, preferably 5 A second step of aging at 20 ° C .; After about 7 days, the composite herbal extract obtained by aging in the second step is placed in a dehydrator, and extracted only with a solvent, preferably ethanol, and after drying and cutting the crumb and mung bean powder, about 20 to 80 times the weight of the herbal medicine, Preferably it is placed in about 65 times the volume of water or C ₁ to C ₄ lower alcohols such as ethanol, methanol, etc., preferably ethanol, for 1 to 30 days, preferably 5 to 15 days, 1 to A third step of aging at 50 ° C., preferably 5 to 20 ° C .; After 7 days of the composite herbal extract obtained by aging in the third step, the solvent, preferably ethanol, is extracted with a dehydrator, and after drying and cutting off the mung bean powder and talc, about 6 to 60 times the weight of the herbal medicine, preferably Is placed in about 30 times the volume of water or C ₁ to C ₄ lower alcohols such as ethanol, methanol, etc., preferably ethanol, for 1 to 30 days, preferably 5 to 15 days, 1 to 50 ° C. , Preferably, the fourth step of ripening at 5 to 20 ° C .; 7 days after the mixed herbal extract obtained by aging in the fourth step using a dehydrator for about 1 to 6 hours, preferably 2 to 4 hours, stirring extraction, boiling water extraction, cold extraction, reflux cooling extraction or ultrasonic extraction, etc. Using the extraction method of the extract, preferably, after extracting by cold extract, the resulting herbal extract containing the half seedling which reduced the toxicity obtained by the manufacturing process including the fifth step of filtration, concentration under reduced pressure or drying was carried out. Provided is an external skin pharmaceutical composition for treating and improving vitiligo containing as an active ingredient.

      In addition, the present invention provides a cosmetic composition for improving vitiligo containing the half seedling-containing complex herbal extract obtained by the production method as an active ingredient.

      Halftones as defined herein include Epicauta gorhami Marseue, Lytta vesicaforia Latreille, Mylabris cichorii Linne or Mylabris phalerata Pallas, preferably Epicauta gorhami Marseue It characterized in that).

       As the weight compounding ratio of the extract of the compound containing the half seedlings, half seedlings (2-7): upper leaf (2-7): lettuce (2-7): bamboo leaf (2-7): kill (2-7): rhubarb (2 ~ 7): mung bean powder (55 to 90): talc (1 to 3) weight ratio (w / w), preferably half seedlings (3 to 6): upper lobe (3 to 6): lettuce (3 to 6): bamboo leaf (3 ~ 6): Kill (3 ~ 6): Rhubarb (3 ~ 6): Mung bean powder (65 ~ 85): Talc (1 ~ 2) Weight ratio (w / w) is included.

      The external skin pharmaceutical composition includes a cream, gel, patch, spray, ointment, warning, lotion, linen, pasta or cataplasma formulation.

In addition, the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, pack, soap And a formulation selected from the group consisting of cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

      The composition of the present invention comprises 0.1 to 50% by weight of the extract relative to the total weight of the composition.

     However, the composition as described above is not necessarily limited thereto, and may vary according to the condition and the degree of progression of the patient.

     The skin external pharmaceutical composition containing the half myocardium-containing complex herbal extract of the present invention as an active ingredient is a cream, gel, patch, spray, ointment, warning agent, lotion agent, linen agent, pasta agent as a skin external agent for treatment and improvement of vitiligo. Or it can be prepared and used as a pharmaceutical composition in the form of an external preparation for skin of cataplasmase, but is not limited thereto.

     Preferred dosages of the extracts of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the preferred effect, the extract of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg per day. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

      In addition, it can be used in a variety of cosmetics having the effect of improving vitiligo containing the half seedling-containing complex herbal extract of the present invention as an active ingredient.

      Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. , Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantotenylethyl Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

     The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid , Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecate oleate, octylate decyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2 -Cetostearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol diethylene glycol, dipropylene propylene glycol, di (capryl, capric acid) propylene glycol, Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate, tricitric acid Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

Examples of the silicone-based fats and oils include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, Alkyl modified silicone oil, amino modified silicone oil, etc. are mentioned.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be mentioned.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

Nonionic surfactants are self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphate, alkyloylalkyltaurin salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkyl sulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. Can be.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, wuro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitrohydrol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5% weight with respect to a total weight, More preferably 0.01-3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The half seedling-containing complex herbal extract of the present invention has been prepared by a method of reducing toxicity, which is a cause of side effects seen in previously known compositions for improving vitiligo, and is known to be harmless to humans and to show a non-toxic effect, thereby treating vitiligo and It can be used in external skin pharmaceutical compositions and cosmetic compositions useful for improvement.

        Hereinafter, the present invention will be described in detail by Examples and Experimental Examples.

       However, the following Examples and Experimental Examples are only illustrative of the present invention, and the content of the present invention is not limited to the following Examples and Experimental Examples.

Reference Example  1. Sample Preparation

     Banmyo, upper lobe, baekbaekpi, bamboo leaf, kill, yellow lotus, mung bean powder, talc were purchased from Ashok Korea Co., Ltd. Hanil Electric Shopping Mall (W-100T mini salamyyi) was used.

Comparative Example  One. Half grave  Preparation of Sole Extract

      After drying and cutting the seedling 100g prepared in Reference Example 1 was put into 6500cc of ethanol and aged at room temperature for 7 days to obtain a seedlings alone extract, which was used as a sample of the following experimental example (hereinafter, "BM-C" Named).

Example  One. Half grave  Preparation of Containing Complex Herbal Extracts

Prepared in Reference Example 1 above Half seedlings, upper leaves, and baekpipi, after drying and cutting, 100g, 250g, 250g each was ground in 6500 ml of ethanol and aged for 7 days at room temperature. The mixed herbal extracts obtained here were put in a dehydrator and only ethanol was extracted. Then, 150 g of bamboo leaves and kills were added to 6496 ml of ethanol, and aged at 15 ° C. for 7 days. After 7 days, the mixed composition obtained therein was put in a dehydrator, and only ethanol was extracted. Then, 100 g of rhubarb and 1000 g of mung bean powder were put into 6492 ml of ethanol, and aged at 15 ° C. for 7 days. After 7 days of the mixed composition obtained here, ethanol was extracted by a dehydrator, and then 1000g of mung bean powder and 50 g of talc were added to 6488 ml of ethanol and aged at 15 ° C for 7 days. After 7 days, the mixed composition obtained by using a dehydrator was put into the extractor 100g to 200g each three minutes to extract three times, and then the obtained extract is filtered, concentrated under reduced pressure or dried to extract the half-containing mixed herbal medicine of the present invention Was obtained and used as a sample of the following experimental example (hereinafter, referred to as "BM-T").

Experimental Example  One. Half grave  Determination of Virulence of Vitiligo Containing Complex Herbal Extracts

      In order to measure the improvement effect of vitiligo of BM-T prepared in Example 1 and BM-C prepared in Comparative Example 1, the experiment was performed as follows.

     Application | coating was applied once a day using the cosmetic brush before bedtime, 0.5-0.8 ml of each BM-T from December 1, 2007 to December 28, 2007.

      As a result of the measurement, as shown in Figures 1 to 4, it was confirmed that the blisters in the form of spots appeared on the scalp coated with BM-C of Comparative Example 1 (see Figs. 1 to 4).

      5 to 8, it was confirmed that the scalp coated with the BM-T of Example 1 showed an improvement effect of vitiligo, in which the blisters in the form of spots disappeared over time (FIG. 5 to FIG. 8).

 Through the above experimental results, it was confirmed that the anti-myocardium-containing complex herbal extract of the present invention to reduce the toxicity, reduce the occurrence of blisters, excellent effect of vitiligo.

       Hereinafter, the preparation examples of the composition including the extract of the present invention, but the present invention is not intended to limit it, but is intended to explain in detail only.

Formulation Example 1 Flexible Lotion

Number Raw material Unit (wt%) One BM-T 4.0 2 glycerin 3.0 3 Butylene glycol 2.0 4 Polyoxyethylene (60) Cured Castor Oil 0.5 5 ethanol 6.0 6 Flavoring, preservative, coloring Quantity 7 Purified water Remaining amount Sum 100.0

Formulation Example 2 Nutritional Lotion

number Raw material Unit (wt%) One BM-T 10.0 2 Squalane 4.0 3 Liquid paraffin 3.0 4 Caprylic / Capric Glyceride 4.0 5 Stearic acid 1.0 6 Cetyl alcohol 1.0 7 Glyceryl Monostearate 0.5 8 Polysorbate 60 1.5 9 Sorbitan sesquioleate 0.5 10 Glyceryl Stearate / Pig-100 Stearate 1.0 11 glycerin 3.0 12 Butylene glycol 2.0 13 Carbomer 0.1 14 Triethanolamine 0.1 15 Preservative, coloring, flavoring Quantity 16 Purified water Remaining amount Sum 100.0

Formulation example  3. Nutrition Cream

number Raw material Unit (wt%) One BM-T 10.0 2 Squalane 5.0 3 Liquid paraffin 3.5 4 Caprylic / Capric Glyceride 4.5 5 Stearic acid 1.5 6 Cetyl alcohol 2.0 7 Beeswax 1.0 8 vaseline 2.0 9 Glyceryl Monostearate 1.0 10 Polysorbate 60 1.5 11 Sorbitan sesquioleate 0.5 12 Glyceryl Stearate / Pig-100 Stearate 1.5 13 glycerin 5.0 14 Butylene glycol 5.0 15 Carbomer 0.2 16 Triethanolamine 0.2 17 Preservative, coloring, flavoring Quantity 18 Purified water Remaining amount Sum 100.0

Formulation example  4. Essence

number Raw material Content (% by weight) One  BM-T 0.5 2 Cytostolol 1.70 3 Polyglyceryl 2-oleate 1.50 4 Ceramide 0.7 5 Ceteares-4 1.2 6 cholesterol 1.5 7 Dicetylphosphate 0.4 8 Concentrated glycerin 5.0 9 Sunflower oil 15.0 10 Carboxy Vinyl Polymer 0.2 11 Xanthan Gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Remaining amount system 100.0

Formulation example  5. Hydrophilic Ointment

number Raw material Content (% by weight) Content (% by weight) One  BM-T 0.5 - 2  BM-T - 0.5 3   White vaseline 250 250 4   Stearyl alcohol 220 220 5   Ethyl (or methyl) p-oxybenzoate 0.25 0.25 6   Propylene glycol 120 120 7   Sodium lauryl sulfate 15 15 8   Propyl p-oxybenzoate 0.15 0.15

        1 is a view showing a state on the seventh day of applying the BM-C of Comparative Example 1,

        2 is a view showing a state on the 14th day of applying the BM-C of Comparative Example 1,

        3 is a view showing a state on the 21st day of applying the BM-C of Comparative Example 1,

        4 is a view showing a state on the 38th day of applying the BM-C of Comparative Example 1,

        5 is a view showing a state on the seventh day of applying the BM-T of Example 1,

        6 is a view showing a state on the 14th day of applying the BM-T of Example 1,

        7 is a view showing a state on the 21st day of applying the BM-T of Example 1,

        8 is a view showing a state on the seventh day of applying the BM-T of Example 1,

Claims (7)

After half-drying and cutting the half seedlings, the upper leaves and the baekpipi, the mixture is placed in a volume of water of about 2 to 100 times the weight of the herbal medicine or lower alcohols of C ₁ to C ₄ such as ethanol and methanol and aged at room temperature for 1 to 30 days. First step; The mixed herbal extract obtained by aging in the first step is put in a dehydrator and extracted with only solvent, and dried and shredded bamboo leaves, killed, and then dried in a volume of about 20 to 80 times the weight of the herbal medicine or C ₁ such as ethanol or methanol. To a lower alcohol of C ₄ to a second step of aging at 1 to 50 ℃ for 1 to 30 days; After about 7 days, the mixed herbal extract obtained by aging in the second step is put into a dehydrator, and only the solvent is extracted, and after drying and cutting the crumb and mung bean powder, water or ethanol with a volume of about 20 to 80 times the weight of the crude herb. A third step of aging the C ₁ to C ₄ lower alcohol such as methanol for ₁ day at 1 to 50 ° C .; After 7 days of the mixed herbal extract obtained by aging in the third step, only the solvent is extracted with a dehydrator, and mung bean powder and talc are shaded and shredded, and water of about 20 to 80 times the weight of the herbal medicine or ethanol, methanol, etc. A fourth step of aging at 1 to 50 ° C. for 7 days in the same C ₁ to C ₄ lower alcohol; After 7 days, the mixed herbal extract obtained by aging in the fourth step was obtained using an extraction method such as stirring extraction, boiling water extraction, cold needle extraction, reflux cooling extraction or ultrasonic extraction for about 1 to 6 hours using a dehydrator. External skin pharmacology for the treatment and improvement of vitiligo containing a semimyocardium-containing complex herbal extract with reduced toxicity obtained by a manufacturing process comprising the step of filtration, concentration under reduced pressure or drying a mixed herbal extract as an active ingredient Composition. The method according to claim 1, wherein the half seedlings include Epicauta gorhami Marseue, Lytta vesicaforia Latreille, Mylabris cichorii Linne, or Mylabris phalerata Pallas. Composition. The weight compounding ratio of the seedling-containing complex herbal extract according to claim 1 is half seedlings (2-7): upper leaves (2-7): lettuce leaves (2-7): bamboo leaves (2-7): kill (2-7) : Sulfur (2 ~ 7): Mung bean powder (55 ~ 90): Skin external pharmaceutical composition in weight ratio (w / w) of talc (1 ~ 3). The composition of claim 1, wherein the composition is a cream, gel, patch, spray, ointment, warning, lotion, linen, pasta or cataplasma formulation.      According to claim 1, wherein the external composition for skin, characterized in that the extract comprises 0.1 to 50% by weight based on the total weight of the composition. After half-drying and cutting the half seedlings, the upper leaves and the baekpipi, the mixture is placed in a volume of water of about 2 to 100 times the weight of the herbal medicine or lower alcohols of C ₁ to C ₄ such as ethanol and methanol and aged at room temperature for 1 to 30 days. First step; The mixed herbal extract obtained by aging in the first step is put in a dehydrator and extracted with only solvent, and dried and shredded bamboo leaves, killed, and then dried in a volume of about 20 to 80 times the weight of the herbal medicine or C ₁ such as ethanol or methanol. To a lower alcohol of C ₄ to a second step of aging at 1 to 50 ℃ for 1 to 30 days; After about 7 days, the mixed herbal extract obtained by aging in the second step is put into a dehydrator, and only the solvent is extracted, and after drying and cutting the crumb and mung bean powder, water or ethanol with a volume of about 20 to 80 times the weight of the crude herb. A third step of aging the C ₁ to C ₄ lower alcohol such as methanol for ₁ day at 1 to 50 ° C .; After 7 days of the mixed herbal extract obtained by aging in the third step, only the solvent is extracted with a dehydrator, and mung bean powder and talc are shaded and shredded, and water of about 20 to 80 times the weight of the herbal medicine or ethanol, methanol, etc. A fourth step of aging at 1 to 50 ° C. for 7 days in the same C ₁ to C ₄ lower alcohol; After 7 days, the mixed herbal extract obtained by aging in the fourth step was obtained using an extraction method such as stirring extraction, boiling water extraction, cold needle extraction, reflux cooling extraction or ultrasonic extraction for about 1 to 6 hours using a dehydrator. A cosmetic composition for improving vitiligo, comprising as an active ingredient a semi-myocardium-containing complex herbal extract obtained by the manufacturing process comprising the step of filtration, concentrated under reduced pressure or drying a mixed herbal extract as an active ingredient. According to claim 6, wherein the composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Cosmetic composition, characterized in that the formulation selected from the group consisting of pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

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